license: cc-by-nc-sa-4.0
widget:
- text: ACCTGA<mask>TTCTGAGTC
tags:
- DNA
- biology
- genomics
- segmentation
segment-nt-multi-species
Segment-NT-multi-species is a segmentation model leveraging the Nucleotide Transformer (NT) DNA foundation model to predict the location of several types of genomics elements in a sequence at a single nucleotide resolution. It is the result of finetuning the Segment-NT model on a dataset encompassing the human genome but also the genomes of 5 selected species: mouse, chicken, fly, zebrafish and worm.
For the finetuning on the multi-species genomes, we curated a dataset of a subset of the annotations used to train Segment-NT, mainly because only this subset of annotations is available for these species. The annotations therefore concern the 7 main gene elements available from Ensembl, namely protein-coding gene, 5’UTR, 3’UTR, intron, exon, splice acceptor and donor sites.
Developed by: InstaDeep
Model Sources
- Repository: Nucleotide Transformer
- Paper: Segmenting the genome at single-nucleotide resolution with DNA foundation models TODO: Add link to preprint
How to use
Until its next release, the transformers library needs to be installed from source with the following command in order to use the models:
pip install --upgrade git+https://github.com/huggingface/transformers.git
A small snippet of code is given here in order to retrieve both logits and embeddings from a dummy DNA sequence.
⚠️ The maximum sequence length is set by default at the training length of 30,000 nucleotides, or 5001 tokens (accounting for the CLS token). However, Segment-NT has
been shown to generalize up to sequences of 50,000 bp. In case you need to infer on sequences between 30kbp and 50kbp, make sure to change the `rescaling_factor`
argument in the config to `num_dna_tokens_inference / max_num_tokens_nt` where `num_dna_tokens_inference` is the number of tokens at inference
(i.e 6669 for a sequence of 40008 base pairs) and `max_num_tokens_nt` is the max number of tokens on which the backbone nucleotide-transformer was trained on, i.e `2048`.
# Load model and tokenizer
from transformers import AutoTokenizer, AutoModel
import torch
tokenizer = AutoTokenizer.from_pretrained("InstaDeepAI/segment_nt_multi_species", trust_remote_code=True)
model = AutoModel.from_pretrained("InstaDeepAI/segment_nt_multi_species", trust_remote_code=True)
# Choose the length to which the input sequences are padded. By default, the
# model max length is chosen, but feel free to decrease it as the time taken to
# obtain the embeddings increases significantly with it.
# The number of DNA tokens (excluding the CLS token prepended) needs to be dividible by
# 2 to the power of the number of downsampling block, i.e 4.
max_length = 12 + 1
assert (max_length - 1) % 4 == 0, (
"The number of DNA tokens (excluding the CLS token prepended) needs to be dividible by"
"2 to the power of the number of downsampling block, i.e 4.")
# Create a dummy dna sequence and tokenize it
sequences = ["ATTCCGATTCCGATTCCG", "ATTTCTCTCTCTCTCTGAGATCGATCGATCGAT"]
tokens = tokenizer.batch_encode_plus(sequences, return_tensors="pt", padding="max_length", max_length = max_length)["input_ids"]
# Infer
attention_mask = tokens != tokenizer.pad_token_id
outs = model(
tokens,
attention_mask=attention_mask,
output_hidden_states=True
)
# Obtain the logits over the genomic features
logits = outs.logits.detach()
# Transform them in probabilities
probabilities = torch.nn.functional.softmax(logits, dim=-1)
print(f"Probabilities shape: {probabilities.shape}")
# Get probabilities associated with intron
idx_intron = model.config.features.index("intron")
probabilities_intron = probabilities[:,:,idx_intron]
print(f"Intron probabilities shape: {probabilities_intron.shape}")
Training data
The segment-nt-multi-species model was finetuned on human, mouse, chicken, fly, zebrafish and worm genomes. For each specie, a subset of chromosomes is kept as validation for training monitoring and test for final evaluation.
Training procedure
Preprocessing
The DNA sequences are tokenized using the Nucleotide Transformer Tokenizer, which tokenizes sequences as 6-mers tokens as described in the Tokenization section of the associated repository. This tokenizer has a vocabulary size of 4105. The inputs of the model are then of the form:
<CLS> <ACGTGT> <ACGTGC> <ACGGAC> <GACTAG> <TCAGCA>
Training
The model was finetuned on a DGXH100 node with 8 GPUs on a total of 8B tokens for 3 days.
Architecture
The model is composed of the nucleotide-transformer-v2-500m-multi-species encoder, from which we removed the language model head and replaced it by a 1-dimensional U-Net segmentation head [4] made of 2 downsampling convolutional blocks and 2 upsampling convolutional blocks. Each of these blocks is made of 2 convolutional layers with 1, 024 and 2, 048 kernels respectively. This additional segmentation head accounts for 53 million parameters, bringing the total number of parameters to 562M.
BibTeX entry and citation info
#TODO: Add bibtex citation here