Christina Theodoris
commited on
Commit
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77eb432
1
Parent(s):
4f6115b
Add instructions to convert gene annotations w/ Ensembl Biomart
Browse files
examples/tokenizing_scRNAseq_data.ipynb
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@@ -17,7 +17,7 @@
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"source": [
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"#### Input data is a directory with .loom files containing raw counts from single cell RNAseq data, including all genes detected in the transcriptome without feature selection. \n",
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"\n",
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"#### Genes should be labeled with Ensembl IDs (row attribute \"ensembl_id\"), which provide a unique identifer for conversion to tokens. Cells should be labeled with the total read count in the cell (column attribute \"n_counts\") to be used for normalization.\n",
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"\n",
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"#### No cell metadata is required, but custom cell attributes may be passed onto the tokenized dataset by providing a dictionary of custom attributes to be added, which is formatted as loom_col_attr_name : desired_dataset_col_attr_name. For example, if the original .loom dataset has column attributes \"cell_type\" and \"organ_major\" and one would like to retain these attributes as labels in the tokenized dataset with the new names \"cell_type\" and \"organ\", respectively, the following custom attribute dictionary should be provided: {\"cell_type\": \"cell_type\", \"organ_major\": \"organ\"}. \n",
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"\n",
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"source": [
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"#### Input data is a directory with .loom files containing raw counts from single cell RNAseq data, including all genes detected in the transcriptome without feature selection. \n",
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"\n",
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"#### Genes should be labeled with Ensembl IDs (row attribute \"ensembl_id\"), which provide a unique identifer for conversion to tokens. Other forms of gene annotations (e.g. gene names) can be converted to Ensembl IDs via Ensembl Biomart. Cells should be labeled with the total read count in the cell (column attribute \"n_counts\") to be used for normalization.\n",
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"\n",
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"#### No cell metadata is required, but custom cell attributes may be passed onto the tokenized dataset by providing a dictionary of custom attributes to be added, which is formatted as loom_col_attr_name : desired_dataset_col_attr_name. For example, if the original .loom dataset has column attributes \"cell_type\" and \"organ_major\" and one would like to retain these attributes as labels in the tokenized dataset with the new names \"cell_type\" and \"organ\", respectively, the following custom attribute dictionary should be provided: {\"cell_type\": \"cell_type\", \"organ_major\": \"organ\"}. \n",
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"\n",
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