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{"start": 751.69, "end": 752.09, "word": " عندي", "probability": 0.793212890625}, {"start": 752.09, "end": 753.29, "word": " Vegas", "probability": 0.270751953125}, {"start": 753.29, "end": 753.63, "word": " nerve", "probability": 0.87255859375}], "temperature": 1.0}, {"id": 30, "seek": 77524, "start": 754.88, "end": 775.24, "text": "هذا هو ال Vegas nerve وهذا هو left Vegas nerve وهنا عندي اللي هو right Vegas nerve مقلوبة صورة بصير view الاختلاف فيهم انه في عصب اسمه recurrent laryngeal nerve ال right recurrent laryngeal nerve", "tokens": [3224, 15730, 31439, 2423, 15841, 16355, 37037, 15730, 31439, 1411, 15841, 16355, 4032, 3224, 8315, 18871, 16254, 13672, 1829, 31439, 558, 15841, 16355, 3714, 4587, 1211, 37746, 3660, 20328, 13063, 3660, 4724, 9381, 13546, 1910, 2423, 47283, 2655, 15040, 5172, 8978, 16095, 16472, 3224, 8978, 6225, 9381, 3555, 24525, 2304, 3224, 18680, 1753, 287, 39008, 432, 304, 16355, 2423, 558, 18680, 1753, 287, 39008, 432, 304, 16355], "avg_logprob": 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79730, "start": 775.52, "end": 797.3, "text": "بيعمل hooking بيلف و بيرجع من الخلف بتاع ال posterior part of the subclavian artery يبقى نقل هو right recurrent laryngeal nerve بيعمل hooking من وين من اللي هو right subclavian artery أما ال ..", "tokens": [21292, 25957, 1211, 6328, 278, 4724, 1829, 46538, 4032, 4724, 13546, 7435, 3615, 9154, 33962, 46538, 39894, 45761, 2423, 33529, 644, 295, 264, 1422, 3474, 43108, 38520, 7251, 3555, 4587, 7578, 8717, 4587, 1211, 31439, 558, 18680, 1753, 287, 39008, 432, 304, 16355, 4724, 1829, 25957, 1211, 6328, 278, 9154, 4032, 9957, 9154, 13672, 1829, 31439, 558, 1422, 3474, 43108, 38520, 5551, 15042, 2423, 4386], "avg_logprob": -0.22194601821176935, "compression_ratio": 1.4795321637426901, "no_speech_prob": 5.960464477539063e-08, "words": [{"start": 775.52, "end": 776.28, "word": "بيعمل", "probability": 0.72412109375}, {"start": 776.28, "end": 776.72, "word": " hooking", "probability": 0.542724609375}, {"start": 776.72, "end": 777.46, "word": " بيلف", 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عليناك بيغدي two muscles Sternocleidomastoid muscle و Trapezius muscle okay", "tokens": [3555, 2655, 24401, 4032, 44945, 5172, 36520, 18871, 2423, 30617, 4032, 4724, 32887, 5016, 45164, 7649, 20449, 2423, 15841, 36536, 69, 20449, 2423, 15841, 4032, 1738, 2071, 3101, 310, 7251, 3215, 1211, 4587, 3714, 33599, 1829, 8315, 25894, 8315, 4117, 4724, 1829, 17082, 16254, 732, 9530, 745, 1248, 905, 306, 327, 298, 525, 17079, 8679, 4032, 5403, 494, 3992, 301, 8679, 1392], "avg_logprob": -0.3477182596448868, "compression_ratio": 1.3235294117647058, "no_speech_prob": 0.0, "words": [{"start": 1233.75, "end": 1234.33, "word": "بتحد", "probability": 0.6886393229166666}, {"start": 1234.33, "end": 1234.69, "word": " و", "probability": 0.473388671875}, {"start": 1234.69, "end": 1235.19, "word": " بنفصل", "probability": 0.8880208333333334}, {"start": 1235.19, "end": 1235.41, "word": " عن", "probability": 0.97412109375}, {"start": 1235.41, "end": 1235.49, "word": " ال", "probability": 0.91650390625}, 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0.5166015625}, {"start": 1431.13, "end": 1431.35, "word": " right", "probability": 0.9375}, {"start": 1431.35, "end": 1431.45, "word": " أو", "probability": 0.6171875}, {"start": 1431.45, "end": 1431.59, "word": " ال", "probability": 0.8623046875}, {"start": 1431.59, "end": 1431.83, "word": " left", "probability": 0.97216796875}], "temperature": 1.0}, {"id": 58, "seek": 145527, "start": 1433.45, "end": 1455.27, "text": "branches of hypoglossal nerve itself هلأ ال hypoglossal nerve بتحد مع C1 و C2 و بيكونون حاجة اسمها answer cervicals أنا مارح أتطرق للموضوع هذا لإنه أنا بشرح للطب أكتر أنه بتحد مع cervical one nerve و cervical two أنا راح أشرح بس إيش العضلات اللي بيغديهم ال hypoglossal nerve itself", "tokens": [1443, 282, 3781, 295, 7420, 664, 75, 772, 304, 16355, 2564, 8032, 1211, 10721, 2423, 7420, 664, 75, 772, 304, 16355, 39894, 24401, 20449, 383, 16, 4032, 383, 17, 4032, 4724, 1829, 30544, 11536, 11331, 26108, 3660, 24525, 2304, 11296, 1867, 49883, 82, 41850, 3714, 9640, 5016, 5551, 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"probability": 0.6982421875}, {"start": 1441.91, "end": 1442.13, "word": " answer", "probability": 0.277587890625}, {"start": 1442.13, "end": 1442.83, "word": " cervicals", "probability": 0.53594970703125}, {"start": 1442.83, "end": 1442.97, "word": " أنا", "probability": 0.451904296875}, {"start": 1442.97, "end": 1443.33, "word": " مارح", "probability": 0.8098958333333334}, {"start": 1443.33, "end": 1444.05, "word": " أتطرق", "probability": 0.85849609375}, {"start": 1444.05, "end": 1444.63, "word": " للموضوع", "probability": 0.9646484375}, {"start": 1444.63, "end": 1444.89, "word": " هذا", "probability": 0.875}, {"start": 1444.89, "end": 1445.29, "word": " لإنه", "probability": 0.8509521484375}, {"start": 1445.29, "end": 1445.67, "word": " أنا", "probability": 0.60009765625}, {"start": 1445.67, "end": 1445.97, "word": " بشرح", "probability": 0.94287109375}, {"start": 1445.97, "end": 1446.35, "word": " للطب", "probability": 0.9148763020833334}, {"start": 1446.35, "end": 1446.73, "word": " أكتر", "probability": 0.9486083984375}, {"start": 1446.73, "end": 1447.41, "word": " أنه", "probability": 0.5826416015625}, {"start": 1447.41, "end": 1447.75, "word": " بتحد", "probability": 0.923095703125}, {"start": 1447.75, "end": 1447.95, "word": " مع", "probability": 0.9189453125}, {"start": 1447.95, "end": 1448.55, "word": " cervical", "probability": 0.78955078125}, {"start": 1448.55, "end": 1449.11, "word": " one", "probability": 0.41650390625}, {"start": 1449.11, "end": 1449.53, "word": " nerve", "probability": 0.83154296875}, {"start": 1449.53, "end": 1449.65, "word": " و", "probability": 0.9755859375}, {"start": 1449.65, "end": 1449.97, "word": " cervical", "probability": 0.908203125}, {"start": 1449.97, "end": 1450.41, "word": " two", "probability": 0.8994140625}, {"start": 1450.41, "end": 1451.05, "word": " أنا", "probability": 0.57421875}, {"start": 1451.05, "end": 1451.25, "word": " راح", "probability": 0.700439453125}, {"start": 1451.25, "end": 1451.69, "word": " أشرح", "probability": 0.9695638020833334}, {"start": 1451.69, "end": 1452.09, "word": " بس", "probability": 0.97119140625}, {"start": 1452.09, "end": 1452.57, "word": " إيش", "probability": 0.861328125}, {"start": 1452.57, "end": 1452.95, "word": " العضلات", "probability": 0.9935302734375}, {"start": 1452.95, "end": 1453.05, "word": " اللي", "probability": 0.94287109375}, {"start": 1453.05, "end": 1453.53, "word": " بيغديهم", "probability": 0.8429361979166666}, {"start": 1453.53, "end": 1453.63, "word": " ال", "probability": 0.9326171875}, {"start": 1453.63, "end": 1454.29, "word": " hypoglossal", "probability": 0.9611328125}, {"start": 1454.29, "end": 1454.53, "word": " nerve", "probability": 0.9189453125}, {"start": 1454.53, "end": 1455.27, "word": " itself", "probability": 0.8037109375}], "temperature": 1.0}, {"id": 59, "seek": 148395, "start": 1455.75, "end": 1483.95, "text": "اللي أصله جاي من الـ hypoglossal nucleus الأصله جاي من وين؟ من ميدالة أبلانجادا الـ supply is styloglossus muscle, a hyoglossus muscle وgenioglossus muscle and the internal muscle of the tongue يبقى من ال fibers جاية من ال hypoglossal nucleus اللي أعطتنا hypoglossal nerve itself بدون ما يشارك مع ال spinal nerves بيغدينا styloglossus muscle", "tokens": [6027, 20292, 5551, 36520, 3224, 10874, 47302, 9154, 2423, 39184, 7420, 664, 75, 772, 304, 28055, 16247, 36520, 3224, 10874, 47302, 9154, 4032, 9957, 22807, 9154, 3714, 25708, 6027, 3660, 5551, 36150, 7649, 7435, 18513, 995, 2423, 39184, 5847, 307, 7952, 752, 7191, 772, 301, 8679, 11, 257, 2477, 664, 75, 772, 301, 8679, 4032, 1766, 72, 664, 75, 772, 301, 8679, 293, 264, 6920, 8679, 295, 264, 10601, 7251, 3555, 4587, 7578, 9154, 2423, 25252, 10874, 995, 10632, 9154, 2423, 7420, 664, 75, 772, 304, 28055, 13672, 1829, 5551, 3615, 9566, 2655, 8315, 7420, 664, 75, 772, 304, 16355, 2564, 47525, 11536, 19446, 7251, 8592, 9640, 4117, 20449, 2423, 28022, 23078, 4724, 1829, 17082, 3215, 9957, 995, 7952, 752, 7191, 772, 301, 8679], 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1479.59, "end": 1479.75, "word": " ما", "probability": 0.95849609375}, {"start": 1479.75, "end": 1480.21, "word": " يشارك", "probability": 0.9930419921875}, {"start": 1480.21, "end": 1480.41, "word": " مع", "probability": 0.9775390625}, {"start": 1480.41, "end": 1480.53, "word": " ال", "probability": 0.91943359375}, {"start": 1480.53, "end": 1480.87, "word": " spinal", "probability": 0.96533203125}, {"start": 1480.87, "end": 1481.39, "word": " nerves", "probability": 0.82275390625}, {"start": 1481.39, "end": 1482.67, "word": " بيغدينا", "probability": 0.6890869140625}, {"start": 1482.67, "end": 1483.63, "word": " styloglossus", "probability": 0.86025390625}, {"start": 1483.63, "end": 1483.95, "word": " muscle", "probability": 0.97314453125}], "temperature": 1.0}, {"id": 60, "seek": 149673, "start": 1485.07, "end": 1496.73, "text": "we hyoglossus muscle و we genioglossus muscle and internal muscle of the tongue هاي عندي واضحة hyoglossal إيه نكلياس", "tokens": [826, 2477, 664, 75, 772, 301, 8679, 4032, 321, 1049, 72, 664, 75, 772, 301, 8679, 293, 6920, 8679, 295, 264, 10601, 8032, 47302, 18871, 16254, 4032, 46958, 5016, 3660, 2477, 664, 75, 772, 304, 11933, 1829, 3224, 8717, 4117, 20292, 32277], "avg_logprob": -0.35428777822228363, "compression_ratio": 1.1896551724137931, "no_speech_prob": 0.0, "words": [{"start": 1485.07, "end": 1485.37, "word": "we", "probability": 0.13330078125}, {"start": 1485.37, "end": 1486.21, "word": " hyoglossus", "probability": 0.770751953125}, {"start": 1486.21, "end": 1486.61, "word": " muscle", "probability": 0.81982421875}, {"start": 1486.61, "end": 1487.15, "word": " و", "probability": 0.6826171875}, {"start": 1487.15, "end": 1487.25, "word": " we", "probability": 0.28515625}, {"start": 1487.25, "end": 1488.29, "word": " genioglossus", "probability": 0.8380126953125}, {"start": 1488.29, "end": 1488.81, "word": " muscle", "probability": 0.9580078125}, {"start": 1488.81, "end": 1489.57, "word": " and", "probability": 0.7490234375}, {"start": 1489.57, "end": 1490.43, "word": " internal", "probability": 0.87548828125}, {"start": 1490.43, "end": 1491.19, "word": " muscle", "probability": 0.8701171875}, {"start": 1491.19, "end": 1491.61, "word": " of", "probability": 0.9765625}, {"start": 1491.61, "end": 1491.79, "word": " the", "probability": 0.8623046875}, {"start": 1491.79, "end": 1492.17, "word": " tongue", "probability": 0.92724609375}, {"start": 1492.17, "end": 1492.95, "word": " هاي", "probability": 0.5589599609375}, {"start": 1492.95, "end": 1493.29, "word": " عندي", "probability": 0.80029296875}, {"start": 1493.29, "end": 1494.59, "word": " واضحة", "probability": 0.9736328125}, {"start": 1494.59, "end": 1495.35, "word": " hyoglossal", "probability": 0.745263671875}, {"start": 1495.35, "end": 1495.77, "word": " إيه", "probability": 0.7489420572916666}, {"start": 1495.77, "end": 1496.73, "word": " نكلياس", "probability": 0.7103271484375}], "temperature": 1.0}, {"id": 61, "seek": 152195, "start": 1497.45, "end": 1521.95, "text": "عطتنا hypoglossal nerve بيفوت في ال hypoglossal canal زى ما قلنا بالتحد مع c1 و c2 و c3 ما راح اتدخل و اتكلم فى الموضوع حدا و اتطرق إله لأنه الموضوع شوية صعب راح يكون عليكوا لكن ال hypoglossal nerve itself بيغدىنا external and internal muscle of the tongue اللى اتكلمنا عنهم تلاتة اللى هو styroglossus muscle, hyoglossus muscle و geneoglossus muscle", "tokens": [3615, 9566, 2655, 8315, 7420, 664, 75, 772, 304, 16355, 4724, 33911, 35473, 8978, 2423, 7420, 664, 75, 772, 304, 9911, 30767, 7578, 19446, 12174, 1211, 8315, 20666, 2655, 5016, 3215, 20449, 269, 16, 4032, 269, 17, 4032, 269, 18, 19446, 12602, 39319, 1975, 2655, 3215, 9778, 1211, 4032, 1975, 2655, 4117, 19528, 6156, 7578, 9673, 2407, 11242, 45367, 11331, 28259, 4032, 1975, 2655, 9566, 2288, 4587, 11933, 1211, 3224, 5296, 33456, 3224, 9673, 2407, 11242, 45367, 13412, 2407, 10632, 20328, 3615, 3555, 12602, 39319, 7251, 30544, 25894, 4117, 14407, 44381, 2423, 7420, 664, 75, 772, 304, 16355, 2564, 4724, 1829, 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"end": 1501.15, "word": " canal", "probability": 0.6318359375}, {"start": 1501.15, "end": 1502.05, "word": " زى", "probability": 0.5997314453125}, {"start": 1502.05, "end": 1502.09, "word": " ما", "probability": 0.95849609375}, {"start": 1502.09, "end": 1502.29, "word": " قلنا", "probability": 0.7926432291666666}, {"start": 1502.29, "end": 1502.63, "word": " بالتحد", "probability": 0.615966796875}, {"start": 1502.63, "end": 1502.79, "word": " مع", "probability": 0.9501953125}, {"start": 1502.79, "end": 1503.25, "word": " c1", "probability": 0.6026611328125}, {"start": 1503.25, "end": 1503.69, "word": " و", "probability": 0.939453125}, {"start": 1503.69, "end": 1504.09, "word": " c2", "probability": 0.9189453125}, {"start": 1504.09, "end": 1504.19, "word": " و", "probability": 0.962890625}, {"start": 1504.19, "end": 1504.59, "word": " c3", "probability": 0.937255859375}, {"start": 1504.59, "end": 1504.85, "word": " ما", "probability": 0.73046875}, {"start": 1504.85, "end": 1505.03, "word": " راح", "probability": 0.4725341796875}, {"start": 1505.03, "end": 1505.57, "word": " اتدخل", "probability": 0.865966796875}, {"start": 1505.57, "end": 1505.87, "word": " و", "probability": 0.90869140625}, {"start": 1505.87, "end": 1506.17, "word": " اتكلم", "probability": 0.9326171875}, {"start": 1506.17, "end": 1506.27, "word": " فى", "probability": 0.7054443359375}, {"start": 1506.27, "end": 1506.61, "word": " الموضوع", "probability": 0.99853515625}, {"start": 1506.61, "end": 1506.91, "word": " حدا", "probability": 0.60235595703125}, {"start": 1506.91, "end": 1507.03, "word": " و", "probability": 0.9072265625}, {"start": 1507.03, "end": 1507.41, "word": " اتطرق", "probability": 0.92587890625}, {"start": 1507.41, "end": 1507.63, "word": " إله", "probability": 0.4962158203125}, {"start": 1507.63, "end": 1508.07, "word": " لأنه", "probability": 0.678955078125}, {"start": 1508.07, "end": 1508.57, "word": " الموضوع", "probability": 0.96533203125}, {"start": 1508.57, "end": 1508.95, "word": " شوية", "probability": 0.794921875}, {"start": 1508.95, "end": 1509.55, "word": " صعب", "probability": 0.8662109375}, {"start": 1509.55, "end": 1509.67, "word": " راح", "probability": 0.861083984375}, {"start": 1509.67, "end": 1509.83, "word": " يكون", "probability": 0.980224609375}, {"start": 1509.83, "end": 1510.33, "word": " عليكوا", "probability": 0.7737630208333334}, {"start": 1510.33, "end": 1510.79, "word": " لكن", "probability": 0.7568359375}, {"start": 1510.79, "end": 1510.99, "word": " ال", "probability": 0.9404296875}, {"start": 1510.99, "end": 1511.65, "word": " hypoglossal", "probability": 0.95322265625}, {"start": 1511.65, "end": 1512.57, "word": " nerve", "probability": 0.4990234375}, {"start": 1512.57, "end": 1513.03, "word": " itself", "probability": 0.2479248046875}, {"start": 1513.03, "end": 1513.65, "word": " بيغدىنا", "probability": 0.7831217447916666}, {"start": 1513.65, "end": 1514.13, "word": " external", "probability": 0.841796875}, {"start": 1514.13, "end": 1514.51, "word": " and", "probability": 0.81396484375}, {"start": 1514.51, "end": 1514.89, "word": " internal", "probability": 0.890625}, {"start": 1514.89, "end": 1515.27, "word": " muscle", "probability": 0.75341796875}, {"start": 1515.27, "end": 1515.39, "word": " of", "probability": 0.9599609375}, {"start": 1515.39, "end": 1515.51, "word": " the", "probability": 0.9130859375}, {"start": 1515.51, "end": 1515.83, "word": " tongue", "probability": 0.9384765625}, {"start": 1515.83, "end": 1516.23, "word": " اللى", "probability": 0.951904296875}, {"start": 1516.23, "end": 1516.69, "word": " اتكلمنا", "probability": 0.9244140625}, {"start": 1516.69, "end": 1517.11, "word": " عنهم", "probability": 0.90771484375}, {"start": 1517.11, "end": 1517.93, "word": " تلاتة", "probability": 0.8343505859375}, {"start": 1517.93, "end": 1518.35, "word": " اللى", "probability": 0.925048828125}, {"start": 1518.35, "end": 1518.59, "word": " هو", "probability": 0.7099609375}, {"start": 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30544, 1975, 8315, 1975, 2407, 445, 5296, 24793, 4032, 13412, 2288, 33753, 5296, 24793, 2423, 39685, 831, 23078, 28242, 16095, 4032, 11778, 20498, 39237, 4724, 9778, 13546, 4032, 21984, 37495, 9566, 1829, 24793], "avg_logprob": -0.3292214870452881, "compression_ratio": 1.349112426035503, "no_speech_prob": 1.0967254638671875e-05, "words": [{"start": 1522.36, "end": 1522.8, "word": "and", "probability": 0.525390625}, {"start": 1522.8, "end": 1523.18, "word": " supply", "probability": 0.93359375}, {"start": 1523.18, "end": 1527.96, "word": " all", "probability": 0.9248046875}, {"start": 1527.96, "end": 1528.36, "word": " internal", "probability": 0.60693359375}, {"start": 1528.36, "end": 1528.78, "word": " muscles", "probability": 0.49755859375}, {"start": 1528.78, "end": 1529.08, "word": " of", "probability": 0.96533203125}, {"start": 1529.08, "end": 1529.24, "word": " the", "probability": 0.87255859375}, {"start": 1529.24, "end": 1529.6, "word": " tongue", "probability": 0.88671875}, {"start": 1529.6, "end": 1530.96, "word": " بطريقة", "probability": 0.78814697265625}, {"start": 1530.96, "end": 1531.34, "word": " هادية", "probability": 0.7960611979166666}, {"start": 1531.34, "end": 1531.6, "word": " او", "probability": 0.58575439453125}, {"start": 1531.6, "end": 1532.5, "word": " لحد", "probability": 0.7861328125}, {"start": 1532.5, "end": 1532.7, "word": " هذا", "probability": 0.91357421875}, {"start": 1532.7, "end": 1532.78, "word": " ال", "probability": 0.8271484375}, {"start": 1532.78, "end": 1533.02, "word": " slide", "probability": 0.68505859375}, {"start": 1533.02, "end": 1533.44, "word": " بكون", "probability": 0.6680908203125}, {"start": 1533.44, "end": 1534.22, "word": " انا", "probability": 0.92626953125}, {"start": 1534.22, "end": 1534.7, "word": " او", "probability": 0.4649658203125}, {"start": 1534.7, "end": 1534.92, "word": " just", "probability": 0.350830078125}, {"start": 1534.92, "end": 1535.26, "word": " لكم", "probability": 0.751220703125}, {"start": 1535.26, "end": 1535.36, "word": " و", "probability": 0.98046875}, {"start": 1535.36, "end": 1535.62, "word": " شرحت", "probability": 0.7351888020833334}, {"start": 1535.62, "end": 1536.3, "word": " لكم", "probability": 0.702880859375}, {"start": 1536.3, "end": 1536.66, "word": " ال", "probability": 0.43115234375}, {"start": 1536.66, "end": 1537.08, "word": " cranial", "probability": 0.848876953125}, {"start": 1537.08, "end": 1537.44, "word": " nerves", "probability": 0.92822265625}, {"start": 1537.44, "end": 1537.96, "word": " كلهم", "probability": 0.97216796875}, {"start": 1537.96, "end": 1539.06, "word": " و", "probability": 0.861328125}, {"start": 1539.06, "end": 1539.54, "word": " دومتم", "probability": 0.921875}, {"start": 1539.54, "end": 1540.06, "word": " بخير", "probability": 0.9977213541666666}, {"start": 1540.06, "end": 1540.58, "word": " و", "probability": 0.59326171875}, {"start": 1540.58, "end": 1540.72, "word": " الله", "probability": 0.93212890625}, {"start": 1540.72, "end": 1541.04, "word": " يعطيكم", "probability": 0.93115234375}], "temperature": 1.0}], "language": "ar", "language_probability": 1.0, "duration": 1541.526375, "duration_after_vad": 1457.4193749999988}
\ No newline at end of file
diff --git a/PL9fwy3NUQKwYBFfMQ7WkgiguUA_8aXeXd/UOCjmLy2b44_raw.srt b/PL9fwy3NUQKwYBFfMQ7WkgiguUA_8aXeXd/UOCjmLy2b44_raw.srt
new file mode 100644
index 0000000000000000000000000000000000000000..95bfa8938425822e38f419dc0cc8b0c7944b8c0f
--- /dev/null
+++ b/PL9fwy3NUQKwYBFfMQ7WkgiguUA_8aXeXd/UOCjmLy2b44_raw.srt
@@ -0,0 +1,876 @@
+1
+00:00:04,980 --> 00:00:08,240
+بسم الله الرحمن الرحيم الجزء الثاني من محاضرة
+
+2
+00:00:08,240 --> 00:00:13,440
+لسبوع الثامن نتكلم على Anatomy of Trigeminal nerve
+
+3
+00:00:13,440 --> 00:00:18,000
+Trigeminal nerve is mixed nerve mixed nerve
+
+4
+00:00:18,000 --> 00:00:22,200
+contains motor fibers and sensory fibers
+
+5
+00:00:22,200 --> 00:00:30,040
+Trigeminal nerve is more important than sensory هو
+
+6
+00:00:30,040 --> 00:00:36,540
+أهم شيء له sensory functionو بتكون من two roots
+
+7
+00:00:36,540 --> 00:00:41,880
+one root sensory و another root motor ال sensory
+
+8
+00:00:41,880 --> 00:00:47,720
+root هو ال large root طيب it carry من ناحية
+
+9
+00:00:47,720 --> 00:00:53,680
+sensation pain touch and temperature sensation
+
+10
+00:00:53,680 --> 00:00:58,040
+يبقى أنا بتكلم على superficial sensationand
+
+11
+00:00:58,040 --> 00:01:05,300
+proprioception او deep sensation deep sensation it
+
+12
+00:01:05,300 --> 00:01:10,400
+is fixed cranial nerve يبقى ان هو اكبر في ناحية ال
+
+13
+00:01:10,400 --> 00:01:15,380
+cranial nerve it is fixed cranial nerve and arise
+
+14
+00:01:15,380 --> 00:01:24,900
+بخرج من ventral lateral surface of the pons بخرج
+
+15
+00:01:24,900 --> 00:01:31,460
+مين قلنا من ال ponsas two roots إله two roots
+
+16
+00:01:31,460 --> 00:01:37,840
+large root sensory root و small root motor root
+
+17
+00:01:37,840 --> 00:01:43,020
+يبقى إله two roots one sensory large و one motor
+
+18
+00:01:43,020 --> 00:01:49,400
+small يبقى ال A تراجيب من ال nerve it is the fifth
+
+19
+00:01:49,400 --> 00:01:52,840
+cranial nerve العصب الخامس it is the sixth cranial
+
+20
+00:01:52,840 --> 00:01:56,870
+nerveالو sensory function and motor function the
+
+21
+00:01:56,870 --> 00:02:00,690
+main function is sensory الو two root one root
+
+22
+00:02:00,690 --> 00:02:06,210
+large sensory و second root يعتبر small root motor
+
+23
+00:02:06,210 --> 00:02:10,830
+immerse from the ventral lateral surface of the
+
+24
+00:02:10,830 --> 00:02:20,550
+palms ايوة عندي هاي ال root الكبير اللي هو large
+
+25
+00:02:20,550 --> 00:02:28,970
+root يعتبر sensoryوها اسمها ال route motor
+
+26
+00:02:28,970 --> 00:02:33,770
+نفس الشيء على ال right side بخرج مين؟ من ال
+
+27
+00:02:33,770 --> 00:02:39,990
+ventral lateral service of the pons then pass بيمر
+
+28
+00:02:39,990 --> 00:02:46,090
+في شيء اسمه cerebellopontine angle بيمر وين؟ في
+
+29
+00:02:46,090 --> 00:02:51,250
+angle زاوية اسمها cerebellopontine angle مين بيمر
+
+30
+00:02:51,250 --> 00:02:53,330
+في ال cerebellopontine angle؟
+
+31
+00:02:57,860 --> 00:03:06,040
+العصب الخامس والعصب السابع والعصب الثامن يبقى
+
+32
+00:03:06,040 --> 00:03:11,010
+العصب السابعأو الخامس والسبع والثامن عضوية الثلاثة
+
+33
+00:03:11,010 --> 00:03:16,910
+بيمروا اين في cerebellopontal angle هي عبارة عن
+
+34
+00:03:16,910 --> 00:03:22,090
+زاوية تقع بين ال pons و ال cerebellum بين ال pons
+
+35
+00:03:22,090 --> 00:03:30,150
+و ال cerebellum هاي عندي trigeminal
+
+36
+00:03:30,150 --> 00:03:35,110
+nerve تمام ارسمه بلون أحمر
+
+37
+00:03:38,610 --> 00:03:43,990
+هذه Trigeminal nerve بعدين بيسلم شئ اسمه
+
+38
+00:03:43,990 --> 00:03:48,570
+Trigeminal Ganglion Trigeminal Ganglion او بيسموها
+
+39
+00:03:48,570 --> 00:03:52,910
+Semilunar Ganglion هذه بتقسم ل Trigeminal nerve
+
+40
+00:03:52,910 --> 00:03:57,830
+العصب الخامس لثلاثة
+
+41
+00:03:57,830 --> 00:04:05,210
+Ophthalmic nerve Maxillary nerve و
+
+42
+00:04:07,440 --> 00:04:13,060
+مانديبولار نيرف تفاجنا الوفثال ميكنيرف بيروح لل
+
+43
+00:04:13,060 --> 00:04:17,660
+superior orbital facial ماجزيلري نيرف بيروح ل
+
+44
+00:04:19,410 --> 00:04:22,790
+Rotundum Foramen، Mandibular nerve لـ Oval Foramen
+
+45
+00:04:22,790 --> 00:04:26,050
+أنا مارح أتكلم معكم في العلاج الطبيعي بالتفصيل على
+
+46
+00:04:26,050 --> 00:04:29,490
+الـ Trigeminal nerve أنا بدرسه لطب الأسنان تشريح
+
+47
+00:04:29,490 --> 00:04:32,750
+العصاب لطب الأسنان بياخد معنى في شرح هذا العصاب
+
+48
+00:04:32,750 --> 00:04:37,910
+لوحده ساعتين و نص ساعتين و نص حوالي تقريبا مية و
+
+49
+00:04:37,910 --> 00:04:41,190
+أربعين دقيقة يعني مية و عشرين دقيقة و بيكفيش و
+
+50
+00:04:41,190 --> 00:04:44,690
+أخلي جزء منه للمحاضرة اللي بعدها بشرح كل ال
+
+51
+00:04:44,690 --> 00:04:47,820
+branches لحد ما أصلى كل سن بسنهلكن هنا أنا راح
+
+52
+00:04:47,820 --> 00:04:51,320
+أتكلم in general على ال large nerve و ال function
+
+53
+00:04:51,320 --> 00:04:58,900
+بتاعتهم يعتبر mixed nerve هذا ال nerve فنتاقل
+
+54
+00:04:58,900 --> 00:05:04,020
+لرسمة ثانية أو صورة ثانية واضح هنا ال origin of
+
+55
+00:05:04,020 --> 00:05:07,440
+the trigeminal nerve emerged from the ventral
+
+56
+00:05:07,440 --> 00:05:11,860
+lateral surface of the pons الو نوكلياي هذا ال
+
+57
+00:05:11,860 --> 00:05:15,740
+trigeminal nerve الو نوكليايقلنا هو موجود في الـ
+
+58
+00:05:15,740 --> 00:05:18,860
+Pons لكن في exception لقيله في exception أول
+
+59
+00:05:18,860 --> 00:05:23,840
+nucleus اسمها main sensory nucleus موجودة في الـ
+
+60
+00:05:23,840 --> 00:05:29,960
+Pons ودفتها touch sensation حاسس اللمس اتنين
+
+61
+00:05:29,960 --> 00:05:34,840
+spinal descending nucleus هذه طويلة جزء منها بيمور
+
+62
+00:05:34,840 --> 00:05:39,270
+في الـ Ponsو جزء في الميدالة أبلانجاتا و جزء في ال
+
+63
+00:05:39,270 --> 00:05:44,250
+cervical spinal cord من C1 ل C4 هذي بيسموها spinal
+
+64
+00:05:44,250 --> 00:05:47,170
+descending nucleus موجودة في الميدالة أبلانجاتا
+
+65
+00:05:47,170 --> 00:05:50,530
+جزء منها بيسعد لل Pons وجزء منها بينزل ل cervical
+
+66
+00:05:50,530 --> 00:05:57,050
+spinal cord upper part مسئول عن pain and
+
+67
+00:05:57,050 --> 00:06:01,430
+temperature sensation pain and temperature
+
+68
+00:06:01,430 --> 00:06:07,480
+sensationثلاثة ميزن سيفليك نوكلياس موجودة في الـ
+
+69
+00:06:07,480 --> 00:06:10,320
+mesencephalon يعني في الـ midbrain وظفتها
+
+70
+00:06:10,320 --> 00:06:15,620
+proprioception and deep sensation واخر واحدة أربعة
+
+71
+00:06:15,620 --> 00:06:20,640
+motor nucleus of trigeminal nerve موجودة في الـ
+
+72
+00:06:20,640 --> 00:06:24,480
+pons بتعطينا motor fibers لمندبلر nerve اللي هو
+
+73
+00:06:24,480 --> 00:06:27,840
+branch من ال trigeminal nerve اللي علاقة بال
+
+74
+00:06:27,840 --> 00:06:31,040
+mastication muscle mastication muscle functions
+
+75
+00:06:31,040 --> 00:06:34,770
+and other functions راح نتكلم عنهايبقى عندي كام
+
+76
+00:06:34,770 --> 00:06:39,350
+nucleus؟ عندي أربعة طب لاحظوا أنتم من الأربعة
+
+77
+00:06:39,350 --> 00:06:43,050
+ثلاثة sensory واحدة اتنين تلاتة هدول sensory
+
+78
+00:06:43,050 --> 00:06:46,690
+نوكلياي وواحدة motor عشان أيه قلنا ال main
+
+79
+00:06:46,690 --> 00:06:49,770
+function اللي تراجع من ال nerve is sensory
+
+80
+00:06:49,770 --> 00:06:54,530
+function ويله two root large root sensory and
+
+81
+00:06:54,530 --> 00:07:02,430
+small root motor هي عندي mesencephalic nucleusهذا
+
+82
+00:07:02,430 --> 00:07:11,810
+البرانش الاساسي وهذا البرانش الاساسي وهذا البرانش
+
+83
+00:07:11,810 --> 00:07:19,150
+الاساسي وهذا البرانش الاساسي وهذا البرانش الاساسي
+
+84
+00:07:19,150 --> 00:07:24,850
+وهذا البرانش الاساسي وهذا البرانش الاساسي وهذا
+
+85
+00:07:24,850 --> 00:07:27,390
+البرانش الاساسي وهذا البرانش الاساسي وهذا البرانش
+
+86
+00:07:27,390 --> 00:07:27,630
+الاساسي وهذا البرانش الاساسي وهذا البرانش الاساسي
+
+87
+00:07:27,630 --> 00:07:27,630
+وهذا البرانش الاساسي وهذا البرانش الاساسي وهذا
+
+88
+00:07:27,630 --> 00:07:27,630
+البرانش الاساسي وهذا البرانش الاساسي وهذا البرانش
+
+89
+00:07:27,630 --> 00:07:27,670
+الاساسي وهذا البرانش الاساسي وهذا البرانش الاساسي
+
+90
+00:07:27,670 --> 00:07:29,770
+وهذا البرانش الاساسي وهذا البرانش الاساسي وهذا
+
+91
+00:07:29,770 --> 00:07:31,370
+البرانش الاساسي وهذا البر
+
+92
+00:07:42,410 --> 00:07:45,810
+الـ branches of trigeminal nerve تقولنا ثلاثة،
+
+93
+00:07:45,810 --> 00:07:49,790
+ophthalmic nerve، pure sensory nerve، بيروح لـ
+
+94
+00:07:49,790 --> 00:07:53,650
+superior orbital fissure، maxillary nerve، pure
+
+95
+00:07:53,650 --> 00:07:57,170
+sensory nerve، بيروح لتروروتندمفورامن، mandibular
+
+96
+00:07:57,170 --> 00:08:03,190
+nerve، is mixed sensory and motor، لووالفورامن،
+
+97
+00:08:03,190 --> 00:08:09,060
+هنا عندي واضحأفثالميك نيرف من أفثالميك لأن ال
+
+98
+00:08:09,060 --> 00:08:14,460
+orbit أكتر راية مانديبولر نيرف مانديبولر نيرف
+
+99
+00:08:14,460 --> 00:08:17,160
+mixed مانديبولر نيرف مزيلاري و لا أفثالميك مزيلاري
+
+100
+00:08:17,160 --> 00:08:21,440
+و لا أفثالميك pure sensory nerve أفثالميك نيرف
+
+101
+00:08:21,440 --> 00:08:26,320
+العصب الأول بتاع من التراجيمنال أو الفرع الأول من
+
+102
+00:08:26,320 --> 00:08:30,460
+التراجيمنال نيرف it is the first branch of the
+
+103
+00:08:30,460 --> 00:08:35,440
+trigeminal nervepurely sensory nerve وظيفته فقط
+
+104
+00:08:35,440 --> 00:08:41,360
+لعلاقة بالإحساس supply skin over forehead في
+
+105
+00:08:41,360 --> 00:08:44,300
+الجبين من الجبهة من الأمام لحد البرايتو اكسيبتال
+
+106
+00:08:44,300 --> 00:08:49,460
+سالكس skin over upper eyelid الجلد بيغضي الجلد في
+
+107
+00:08:49,460 --> 00:08:57,300
+ال upper eyelid ال eyeball conjunctiva والعين
+
+108
+00:08:57,300 --> 00:09:03,090
+والغلاف العين والغدة اللي بتفرز الدمعةnasal mucosa
+
+109
+00:09:03,090 --> 00:09:11,250
+frontal sinus external ear جزء خارج من الأنف with
+
+110
+00:09:11,250 --> 00:09:16,110
+dura mater in the anterior cranial fosse كمان مرة
+
+111
+00:09:16,110 --> 00:09:20,110
+واضيفته في thalamic nerve supply skin over
+
+112
+00:09:20,110 --> 00:09:27,530
+forehead skin over upper eyelid eye ball
+
+113
+00:09:27,530 --> 00:09:33,980
+conjunctiva lacrimal glandNasal Mucosa Frontal
+
+114
+00:09:33,980 --> 00:09:40,100
+Sinus External Ear and Durometer in the anterior
+
+115
+00:09:40,100 --> 00:09:47,440
+cranial fossa هذه المنطقة اللي بيغضيها أوفثالمك
+
+116
+00:09:47,440 --> 00:09:55,220
+البرانش اللي هي باللون الأخضر هذه المنطقة بيغضيها
+
+117
+00:09:55,220 --> 00:10:06,200
+مين أوفثالمك نيرفناخدها بلون تاني بيغضيها
+
+118
+00:10:06,200 --> 00:10:11,740
+maxillary nerve وهنا هذه ال area اللي بيغضيها
+
+119
+00:10:11,740 --> 00:10:21,300
+mandibular nerve هي
+
+120
+00:10:21,300 --> 00:10:25,220
+كتقسيمة ال sensation على ال face من ناحية ال
+
+121
+00:10:25,220 --> 00:10:27,680
+branches of الترائجيمنال nerve
+
+122
+00:10:41,430 --> 00:10:46,800
+ما بديش بسملكوا كل branches بتاعينهمهنا عندى ال
+
+123
+00:10:46,800 --> 00:10:49,980
+branches بتاعين ال ophthalmic nerve هنا maxillary
+
+124
+00:10:49,980 --> 00:10:54,020
+nerve هنا mandibular nerve مش لازم ألكوا بالتفصيل
+
+125
+00:10:54,020 --> 00:10:57,480
+لكن قولتلكوا لأنه لما أشرحوا لطب أو لطب الأسنان
+
+126
+00:10:57,480 --> 00:11:01,020
+خاصة في طب الأسنان بتوسع أكتر شي عندهم في ال
+
+127
+00:11:01,020 --> 00:11:04,160
+trigeminal nerve لإنه اللي علاقة بالأسنان و الفك
+
+128
+00:11:04,160 --> 00:11:09,740
+العلوي و الفك السفلي بنتج ل maxillary nerve it is
+
+129
+00:11:09,740 --> 00:11:17,850
+purely sensory nerveit is the second branch of a
+
+130
+00:11:17,850 --> 00:11:24,030
+trigeminal nerve supply بغدى، maxillary area of
+
+131
+00:11:24,030 --> 00:11:28,530
+skin of the face المنطقة هذه، maxillary teeth
+
+132
+00:11:28,530 --> 00:11:33,210
+الأسنان في الفق العلوي في الأبرج هو ميوكوزا
+
+133
+00:11:33,210 --> 00:11:38,940
+membrane related للمقزلةمقزلري صينس مقزلري صينس
+
+134
+00:11:38,940 --> 00:11:42,980
+اللي في ال bone نفسه ال air sinuses و ديورامتر in
+
+135
+00:11:42,980 --> 00:11:46,740
+the middle cranial fosse يبقى مقزلري nerve يعتبر
+
+136
+00:11:46,740 --> 00:11:51,960
+second branch from the trigeminal nerve يعتبر pure
+
+137
+00:11:51,960 --> 00:11:58,450
+sensory nerve supplyالـ cheek أو الـ anterior part
+
+138
+00:11:58,450 --> 00:12:04,530
+of the face تمام supply الـ maxillary teeth في
+
+139
+00:12:04,530 --> 00:12:08,310
+الأبرجو في الفك العلوي supply mucosa membrane
+
+140
+00:12:08,310 --> 00:12:13,090
+related to maxilla وبيغدينا maxillary air sinus
+
+141
+00:12:13,090 --> 00:12:19,550
+and durameter in the middle cranial fossa وبعدها
+
+142
+00:12:19,550 --> 00:12:24,090
+بيفوت لهنا من الترجمة النهائية بيفوت عن طريق
+
+143
+00:12:24,090 --> 00:12:32,010
+Rotundum Formaeهي عندي اللي هو maxillary nerve و
+
+144
+00:12:32,010 --> 00:12:35,390
+ال branch السبعينه اللي علاقة بالفك ايه بالفك
+
+145
+00:12:35,390 --> 00:12:44,490
+العلوي و maxillary sinus فنتجى
+
+146
+00:12:44,490 --> 00:12:50,050
+لبعدها لموضوع انتشوف
+
+147
+00:12:50,050 --> 00:12:55,630
+على الصورة هذه هى عندي maxillary nerve راح ل
+
+148
+00:12:55,630 --> 00:13:02,880
+Rotundum foramenهنا عندي أوفثالميك نيرف وهذا
+
+149
+00:13:02,880 --> 00:13:06,940
+الأخير التالف اللي هو مانديبولار نيرف مانديبولار
+
+150
+00:13:06,940 --> 00:13:11,000
+نيرف اللي هو يعتبر mix nerve contain motor and
+
+151
+00:13:11,000 --> 00:13:16,360
+sensory fibers هذه
+
+152
+00:13:16,360 --> 00:13:23,440
+ال area بتاخدنا من وين؟ من مقسلري نيرف مقسلري نيرف
+
+153
+00:13:24,480 --> 00:13:30,640
+بنتاج لـ mandibular nerve يعتبر third branch from
+
+154
+00:13:30,640 --> 00:13:34,280
+the trigeminal nerve ال function تبعته mix
+
+155
+00:13:34,280 --> 00:13:40,040
+function motor and sensory function له two root
+
+156
+00:13:40,040 --> 00:13:45,520
+large root sensory and small root motor ال sensory
+
+157
+00:13:45,520 --> 00:13:50,530
+بيخرج من ال trigeminal ganglionوبيفوت بعد هيك ل
+
+158
+00:13:50,530 --> 00:13:55,330
+foramen oval ال motor root هو أصله جاي من وين؟ من
+
+159
+00:13:55,330 --> 00:14:00,530
+the trigeminal motor nucleus in the pons بيتحد مع
+
+160
+00:14:00,530 --> 00:14:06,170
+ال sensory root وين في foramen oval بعد ما يخرج ال
+
+161
+00:14:06,170 --> 00:14:10,250
+mandibular nerve من the trigeminal ganglionال
+
+162
+00:14:10,250 --> 00:14:13,970
+motor fibers تتحد مع ال sensory fibers بتاعة ال
+
+163
+00:14:13,970 --> 00:14:17,550
+mandibular nerve وين بتتحدوا؟ في oval foramen
+
+164
+00:14:17,550 --> 00:14:23,050
+وبيصبح انها mandibular nerve mixed nerve contains
+
+165
+00:14:23,050 --> 00:14:30,350
+motor and sensory fibers هي عندي اي sensory fibers
+
+166
+00:14:30,350 --> 00:14:36,040
+تبعت ايه؟من دي بولر نيرف خرجت من وين من ال
+
+167
+00:14:36,040 --> 00:14:40,220
+trigeminal ganglion وفي الخلف بيكون عندى هاي ال
+
+168
+00:14:40,220 --> 00:14:46,470
+pawns في عندىMotor nucleus of trigeminal nerve
+
+169
+00:14:46,470 --> 00:14:50,470
+الفيبر سبعتها بتمر مع ال trigeminal nerve لما
+
+170
+00:14:50,470 --> 00:14:56,870
+يصلوا لforamen oval يتحدوا مع مين؟ مع العصب الفرع
+
+171
+00:14:56,870 --> 00:15:00,050
+الثالث من العصب الخامس مع ال mandibular nerve وفي
+
+172
+00:15:00,050 --> 00:15:03,930
+الحالة هذه عندي ال mandibular nerve يكون mixed
+
+173
+00:15:03,930 --> 00:15:05,430
+nerve
+
+174
+00:15:08,500 --> 00:15:21,360
+contain motor and sensory fibers okay ننتقل
+
+175
+00:15:21,360 --> 00:15:25,220
+ل sensory function of the mandibular nerve ال
+
+176
+00:15:25,220 --> 00:15:28,720
+sensory function of the mandibular nerve اللي
+
+177
+00:15:28,720 --> 00:15:34,560
+علاقة بال mucosa membrane of the mouth and cheek
+
+178
+00:15:35,500 --> 00:15:39,800
+العلاقة بالـ anterior to third of the tongue بيغضي
+
+179
+00:15:39,800 --> 00:15:43,200
+الـ anterior to third of the tongue مش لل test
+
+180
+00:15:43,200 --> 00:15:46,760
+sensation مش لل تدوّق لا ل general sensation إيش
+
+181
+00:15:46,760 --> 00:15:50,680
+يعني general sensation pain temperature touch
+
+182
+00:15:50,680 --> 00:15:55,460
+sensation deep sensation بيغضي ال lower teeth
+
+183
+00:15:55,460 --> 00:15:59,900
+بيغضي الأسنان في الفقه السفلي بيغضي skin of the
+
+184
+00:15:59,900 --> 00:16:05,880
+lower jawSide of head and skull lateral part بيغدى
+
+185
+00:16:05,880 --> 00:16:12,220
+و MNG with uremeter in the middle او middle
+
+186
+00:16:12,220 --> 00:16:17,640
+cranial fossa هذه ال area الحمراء هذه تُخنون من
+
+187
+00:16:17,640 --> 00:16:24,520
+mandibular maxillary ophthalmic ال lateral part
+
+188
+00:16:24,520 --> 00:16:27,880
+هذا تُخنون من mandibular nerve
+
+189
+00:16:32,160 --> 00:16:34,740
+طب اتكلمنا على sensory function of mandibular
+
+190
+00:16:34,740 --> 00:16:40,020
+nerve الان اتطرق ل motor function of mandibular
+
+191
+00:16:40,020 --> 00:16:43,040
+nerve ال motor function of mandibular nerve
+
+192
+00:16:43,040 --> 00:16:49,780
+بيغدينا four mastication muscle اربع عضلات علاقة
+
+193
+00:16:49,780 --> 00:16:55,550
+بعملية المضغ مين هما؟ temporalis musclemasseter
+
+194
+00:16:55,550 --> 00:16:59,530
+muscle, medial pterygoid muscle, lateral pterygoid
+
+195
+00:16:59,530 --> 00:17:03,310
+muscle يعني أول وظيفة من ناحية الموتور انه supply
+
+196
+00:17:03,310 --> 00:17:07,990
+for mastication muscle temporalis muscle,
+
+197
+00:17:08,850 --> 00:17:14,490
+mastication muscles, lateral and medial pterygoid
+
+198
+00:17:14,490 --> 00:17:18,210
+muscles وبيغدين أربع عضلات ثانية يعني بيغدي ثمان
+
+199
+00:17:18,210 --> 00:17:23,520
+عضلات المنديبولار نيرو mylohyoid muscleAnterior
+
+200
+00:17:23,520 --> 00:17:27,940
+Pile of Digastric Muscle Tensor Pelatoid Muscle
+
+201
+00:17:27,940 --> 00:17:33,500
+وTensor Tempani Muscle هذه العضلات اللي راح
+
+202
+00:17:33,500 --> 00:17:37,440
+شايفينها هنا عندي اللي هي temporalis muscle في
+
+203
+00:17:37,440 --> 00:17:41,760
+عندي deep pterygoid muscle أو deep part of
+
+204
+00:17:41,760 --> 00:17:45,140
+masseter muscle و superficial part of masseter
+
+205
+00:17:45,140 --> 00:17:47,800
+هدول ال masseter muscle اللي لها جزئين اللي لها
+
+206
+00:17:47,800 --> 00:17:49,900
+deep part و superficial part
+
+207
+00:17:53,150 --> 00:17:57,370
+في عندنا هنا اللي هي medial pterygoid muscle و
+
+208
+00:17:57,370 --> 00:18:02,990
+lateral pterygoid muscle في عندي ال واضحة ان انا
+
+209
+00:18:02,990 --> 00:18:08,350
+لمسيت ال muscle تمام وفي ال anterobiliary of
+
+210
+00:18:08,350 --> 00:18:12,130
+digastric muscle وmyelohyoid muscle كل ما دول
+
+211
+00:18:12,130 --> 00:18:17,070
+بياخدوا من motor fibers from mandibular nerve هذه
+
+212
+00:18:17,070 --> 00:18:24,510
+هي الmylohyoid muscle وهنا عندي ال antirepoly of
+
+213
+00:18:24,510 --> 00:18:30,970
+digastric muscle okay في عضلة صغيرة هنا اسمها
+
+214
+00:18:30,970 --> 00:18:35,910
+tensor timbany muscle وفي عضلة هنا tensor palatial
+
+215
+00:18:35,910 --> 00:18:40,670
+muscleكلهم بياخدوا من ال mandibular nerve of the
+
+216
+00:18:40,670 --> 00:18:44,390
+trigeminal nerve من ال motor function و هيك بيكون
+
+217
+00:18:44,390 --> 00:18:49,150
+انا شرحتلكوا ال trigeminal nerve شرحنا النقلياعي
+
+218
+00:18:49,150 --> 00:18:52,550
+بتبعته و ال function تبعتهم و ال branches بتاعتهم
+
+219
+00:18:52,550 --> 00:18:54,970
+و يعطيكوا الف عافية
+
diff --git a/PL9fwy3NUQKwYBFfMQ7WkgiguUA_8aXeXd/aMTvg16Fhkk_raw.srt b/PL9fwy3NUQKwYBFfMQ7WkgiguUA_8aXeXd/aMTvg16Fhkk_raw.srt
new file mode 100644
index 0000000000000000000000000000000000000000..513cae163fb9e9f418b53464740f7ccd69d942f1
--- /dev/null
+++ b/PL9fwy3NUQKwYBFfMQ7WkgiguUA_8aXeXd/aMTvg16Fhkk_raw.srt
@@ -0,0 +1,1416 @@
+1
+00:00:04,980 --> 00:00:08,320
+بسم الله الرحمن الرحيم السلام عليكم ورحمة الله
+
+2
+00:00:08,320 --> 00:00:16,140
+وبركاته محاضرة السبوع الثالث Anatomy and
+
+3
+00:00:16,140 --> 00:00:22,020
+Physiology of Basal Nuclei, Diencephalon and
+
+4
+00:00:22,020 --> 00:00:27,120
+Cerebellum جزء الأول من المحاضرة راح ناخد Basal
+
+5
+00:00:27,120 --> 00:00:32,030
+Nucleiو جزء من ال diencephalon في جزء ثاني من
+
+6
+00:00:32,030 --> 00:00:35,850
+المحاضرة عن كامل ال diencephalon و anatomy of
+
+7
+00:00:35,850 --> 00:00:42,730
+physiology of cerebellum ال basal نوكلياي في الكتب
+
+8
+00:00:42,730 --> 00:00:47,610
+بتلاقوها basal ganglia basal ganglia كلمة ganglia
+
+9
+00:00:47,610 --> 00:00:52,350
+wrong خطأ خطأ مصطلح ال basal ganglia ليه؟ لأن ال
+
+10
+00:00:52,350 --> 00:00:56,560
+gangliaWhat's the definition of ganglia؟ هي عبارة
+
+11
+00:00:56,560 --> 00:01:01,640
+عن composed of nerve cells which is located
+
+12
+00:01:01,640 --> 00:01:07,850
+outside CNS، خارج الجهاز العصبي المركزيمدام هي
+
+13
+00:01:07,850 --> 00:01:11,130
+داخل جهاز عصب المركزي inside of the brain يمكن
+
+14
+00:01:11,130 --> 00:01:15,210
+بسميها Basal Nuclei Basal إيه؟ نوكليا لكن لليوم في
+
+15
+00:01:15,210 --> 00:01:18,690
+الكتب والمراجع بتلاقوا اسمها موجود Basal Ganglia و
+
+16
+00:01:18,690 --> 00:01:22,710
+هذا خطأ كلمة Ganglia تطلق على ال nerve cells
+
+17
+00:01:22,710 --> 00:01:28,630
+الموجودة outside of CNS عبارة عن أيش ال Basal
+
+18
+00:01:28,630 --> 00:01:34,730
+Nuclei؟ عبارة عن collections of grey matter
+
+19
+00:01:36,440 --> 00:01:41,740
+Situated within each cerebral hemisphere deep يبقى
+
+20
+00:01:41,740 --> 00:01:47,460
+ان هم عبارة عن gray matter موجودين داخل ال
+
+21
+00:01:47,460 --> 00:01:52,440
+cerebral hemisphere مع ال white matter ديب بيكونه
+
+22
+00:01:52,440 --> 00:01:57,220
+What's the components of basal nuclei؟ من ايش
+
+23
+00:01:57,220 --> 00:02:00,840
+بتكونوا ال basal nuclei؟ ال basal اللي انا موجودة
+
+24
+00:02:00,840 --> 00:02:05,960
+في ال base of cerebral hemisphereبيتكونوا من كودية
+
+25
+00:02:05,960 --> 00:02:14,360
+نوكلياس هاي واحد ثاني لينتي فورم ال نوكلياس كلمة
+
+26
+00:02:14,360 --> 00:02:20,640
+لينتي فورم سموها عشان تشبه شكل الوتد shape شكل
+
+27
+00:02:20,640 --> 00:02:27,540
+الوتد اللينتي فورم تتكوّل من بيوتامين and جلوباس
+
+28
+00:02:27,540 --> 00:02:31,660
+باليداس يبقى كودية نوكلياس لينتي فورم نوكلياس
+
+29
+00:02:33,270 --> 00:02:36,150
+classroom طلاب بيموتوا فيها هذه ال classroom
+
+30
+00:02:36,150 --> 00:02:39,770
+بحبوها كتير لإن ال function تبعتها unknown مش
+
+31
+00:02:39,770 --> 00:02:42,230
+معروف وظيفتها عشان هيك الطلاب بيحبوا في الحاجات
+
+32
+00:02:42,230 --> 00:02:45,630
+الزيادة بيحبوش يذكروا وظائف و لا إشي لما تقوله ال
+
+33
+00:02:45,630 --> 00:02:47,950
+function تبعتها unknown بيقولك الحمد لله unknown
+
+34
+00:02:47,950 --> 00:02:52,950
+يبقى نظرنا مسكين فيها و أربعة amygdaloid nucleus
+
+35
+00:02:52,950 --> 00:02:58,170
+في الكورونا
+
+36
+00:02:58,170 --> 00:03:03,010
+اللي كت لو طلعنا واضح أن عندي كودية nucleusهنا
+
+37
+00:03:03,010 --> 00:03:09,330
+بيكون عندى ايه اللى هو الانتفار في بيوتامين و
+
+38
+00:03:09,330 --> 00:03:16,790
+globus pallidus نجيب صورة ثانية نشرع عليها هذا
+
+39
+00:03:16,790 --> 00:03:22,970
+axial cut axial cut فى عندى الكودية نوكلياس هذه
+
+40
+00:03:22,970 --> 00:03:28,410
+الكودية لها head و لها body مجصوص مش واضح ال body
+
+41
+00:03:28,410 --> 00:03:35,820
+و في النهاية لها tail كل هذا تابع لمينلكوديات
+
+42
+00:03:35,820 --> 00:03:44,400
+نوكلياس اثنين فى عندى الانتفار من نوكلياس تشبه شكل
+
+43
+00:03:44,400 --> 00:03:55,380
+الوطن دى اللى جزءين جزء اسمه putamen وجزء
+
+44
+00:03:55,380 --> 00:04:01,400
+اسمه globus balidas تمام؟ و
+
+45
+00:04:03,420 --> 00:04:09,880
+في classroom بيحبوها الطلاب كثير و وظيفتها unknown
+
+46
+00:04:09,880 --> 00:04:12,920
+و
+
+47
+00:04:12,920 --> 00:04:17,620
+في نهاية ال tail of كودية nucleus تحت في ال tail
+
+48
+00:04:17,620 --> 00:04:21,840
+of كودية nucleus في ال temporal loop بيكون موجودة
+
+49
+00:04:21,840 --> 00:04:25,300
+amygdaloid nucleus
+
+50
+00:04:33,320 --> 00:04:38,240
+What's the functions of basal nuclei؟ أول function
+
+51
+00:04:38,240 --> 00:04:43,100
+Receive afferent information from the cerebral
+
+52
+00:04:43,100 --> 00:04:47,140
+hemisphere, thalamus, subthalamus and brainstem
+
+53
+00:04:47,140 --> 00:04:52,680
+ثمين Control muscular movement and regulation of
+
+54
+00:04:52,680 --> 00:04:56,260
+voluntary movement العلاقة بالحركات الإيرادية
+
+55
+00:04:56,260 --> 00:05:01,970
+ثلاثةlearning of the motor skills المهارات الحركية
+
+56
+00:05:01,970 --> 00:05:06,710
+مسئولة عن المهارات الحركية اربع لو كنا قاعدين انا
+
+57
+00:05:06,710 --> 00:05:09,930
+كنت في المحاضرة دايما اجي فجأه اخبط هيك الطاولة
+
+58
+00:05:09,930 --> 00:05:13,470
+ايش بيصير معاكوا بتخافوا بترتعبوا بتنقزوا لو كنتوا
+
+59
+00:05:13,470 --> 00:05:17,710
+ماشيين في شارع حدث انفجار بتجري ما بتدري وين بتروح
+
+60
+00:05:17,710 --> 00:05:22,980
+ايش بحدث معاك؟تاكي كارديا ال heart rate بيزيد ال
+
+61
+00:05:22,980 --> 00:05:27,180
+skin بيكون شاحب من ال sympathic innervation بيحصل
+
+62
+00:05:27,180 --> 00:05:30,840
+vazoconstriction بيرتفع الضغط بيزيد ال respiratory
+
+63
+00:05:30,840 --> 00:05:36,080
+rate يبقى ان وظيفتها influence the body response
+
+64
+00:05:36,080 --> 00:05:41,540
+to environmental changes زي اي sense of fire for
+
+65
+00:05:41,540 --> 00:05:46,510
+exampleإيش بيحدث؟ change في ال heart rate، في ال
+
+66
+00:05:46,510 --> 00:05:49,050
+blood pressure، في skin color، في respiratory
+
+67
+00:05:49,050 --> 00:05:52,670
+rate، يبقى حدث انفجار، طب ولا شيء، أنت ماشي في
+
+68
+00:05:52,670 --> 00:05:57,510
+الليل و بتفكر في هذا الدنيا و فجأةضلام مش كهرب نبح
+
+69
+00:05:57,510 --> 00:06:01,190
+عليك كلب فجأة وانت ايه مش منتبه ايش بيصير فيك تجري
+
+70
+00:06:01,190 --> 00:06:05,830
+ما بتدري بيصير عندك ال heart rate زيد بينفجر من
+
+71
+00:06:05,830 --> 00:06:10,450
+كتر بيضخ دم بدك تجري ال respiratory rate بيزيد
+
+72
+00:06:10,450 --> 00:06:14,590
+ضغطك بيرتفع وجهك بيكون شاحب لون أبيضتمام؟ و الشعر
+
+73
+00:06:14,590 --> 00:06:18,970
+بيقف لإنه sympathetic nervous system بتقدي عضلة
+
+74
+00:06:18,970 --> 00:06:22,770
+erector pili muscle اللي بتعمل إيه؟ بتوقف الشعر
+
+75
+00:06:22,770 --> 00:06:24,770
+عشان هيك الحاجة لما بيقولوا شفتوا هذا المنظر و
+
+76
+00:06:24,770 --> 00:06:27,170
+الشعر بيقف صح كلامها من sympathetic
+
+77
+00:06:27,170 --> 00:06:32,510
+hyperinherbation راح نشرحهم في محاضرة ال autonomic
+
+78
+00:06:32,510 --> 00:06:37,670
+nervous systemسمال فانكشنسمال فانكشنسمال
+
+79
+00:06:37,670 --> 00:06:37,790
+فانكشنسمال فانكشنسمال فانكشنسمال فانكشنسمال
+
+80
+00:06:37,790 --> 00:06:40,090
+فانكشنسمال فانكشنسمال فانكشنسمال فانكشنسمال
+
+81
+00:06:40,090 --> 00:06:49,650
+فانكشنسمال فانكشنسمال
+
+82
+00:06:49,650 --> 00:06:50,870
+فانكشنسمال فانكشنسمال فانكشنسمال فانكشنسمال
+
+83
+00:06:50,870 --> 00:06:52,030
+فانكشنسمال فانكشنسمال فانكشنسمال فانكشنسمال
+
+84
+00:06:52,030 --> 00:06:52,030
+فانكشنسمال فانكشنسمال فانكشنسمال فانكشنسمال
+
+85
+00:06:52,030 --> 00:06:52,030
+فانكشنسمال فانكشنسمال فانكشنسمال فانكشنسمال
+
+86
+00:06:52,030 --> 00:06:55,710
+فانكشنسمال فانكشنسمال
+
+87
+00:06:55,710 --> 00:06:59,070
+فانكشنسمال
+
+88
+00:06:59,070 --> 00:07:03,010
+فانكشن
+
+89
+00:07:04,270 --> 00:07:09,690
+وبين موجود related lateral to lateral ventricle
+
+90
+00:07:09,690 --> 00:07:14,210
+and thalamus بيكون موجود lateral من ال lateral
+
+91
+00:07:14,210 --> 00:07:20,250
+ventricle و ال thalamus بتكون من ثلاث أجزاء head
+
+92
+00:07:20,250 --> 00:07:28,690
+of كودية نوكلياbody of كودية نوكلياس tail of كودية
+
+93
+00:07:28,690 --> 00:07:33,870
+نوكلياس يعني بتكون من head رأس و body جسم و tail
+
+94
+00:07:33,870 --> 00:07:39,350
+ذالك و في نهاية ال tail بيكون موجود amygdaloid
+
+95
+00:07:39,350 --> 00:07:47,990
+نوكلياس amygdaloid نوكلياس okay نشوف هنا هذا هو ال
+
+96
+00:07:47,990 --> 00:07:54,660
+head ع الأكزي القط head of كودية نوكلياسهنا بيكون
+
+97
+00:07:54,660 --> 00:08:06,660
+tail of جودة نوكلياس على ال axial cut تشوف
+
+98
+00:08:06,660 --> 00:08:10,920
+صورة من السجتال هي السجتال الأخضر هنا بيكون ال
+
+99
+00:08:10,920 --> 00:08:23,860
+head هنا بيكون ايه ال body هنا بيكون ايشTail وفي
+
+100
+00:08:23,860 --> 00:08:28,360
+نهاية الـ Tail بتكون موجودة ايش اميجدالويد نوكلياس
+
+101
+00:08:28,360 --> 00:08:32,640
+او اميجدالويد body هي جزء من الـ Basal نوكلياية
+
+102
+00:08:32,640 --> 00:08:38,160
+وكان من ايش بتكون كودية نوكلياس بتكون من head body
+
+103
+00:08:38,160 --> 00:08:43,300
+tail وفي ال terminal part of the tail located ايش
+
+104
+00:08:43,300 --> 00:08:47,840
+اميجدالويد نوكلياس بتاعة ال Basal نوكلياية
+
+105
+00:08:50,720 --> 00:08:55,500
+هكذا انا شرحت لكم كودة
+
+106
+00:08:55,500 --> 00:08:59,000
+نوكلياس طب وين موجودة كودة نوكلياس قلنا كودة
+
+107
+00:08:59,000 --> 00:09:02,620
+نوكلياس بتكون موجودة لاترال
+
+108
+00:09:05,850 --> 00:09:10,110
+الثالث او lateral ل lateral ventricle هاي الثلمس و
+
+109
+00:09:10,110 --> 00:09:12,770
+ال body تبعها بيكون هيك بالصورة هذه ال body و كمان
+
+110
+00:09:12,770 --> 00:09:15,690
+بتكون lateral ل ثلمس يبقى ان هي located lateral ل
+
+111
+00:09:15,690 --> 00:09:20,510
+ثلمس و lateral ل ال lateral ventricle يبقى ان ال
+
+112
+00:09:20,510 --> 00:09:24,790
+lateral ventricle و الثلمس بيكونوا medial ل مين؟ ل
+
+113
+00:09:24,790 --> 00:09:31,030
+الكودة النوكلياس ننتقل
+
+114
+00:09:31,030 --> 00:09:37,890
+لجزء ثانيمن Basal nuclei اسمه lentiform nucleus
+
+115
+00:09:37,890 --> 00:09:43,570
+ايش اسمه؟ lentiform nucleus it is a wedge shape
+
+116
+00:09:43,570 --> 00:09:48,770
+mass وانه عبارة عن ايه؟ wedge shape mass of grey
+
+117
+00:09:48,770 --> 00:09:54,650
+matter شكل الوتد، وتدي الشكل موجود وين؟ between
+
+118
+00:09:54,650 --> 00:10:00,960
+the internal capsule mediallyand external capsule
+
+119
+00:10:00,960 --> 00:10:04,640
+laterally وعشان هيك سموا ال external capsule
+
+120
+00:10:04,640 --> 00:10:07,780
+external capsule لأنها located external
+
+121
+00:10:07,780 --> 00:10:11,740
+لـLentiform nucleus ولذلك سموا internal capsule
+
+122
+00:10:11,740 --> 00:10:17,240
+internal لأنها located internal لمن؟ لLentiform
+
+123
+00:10:17,240 --> 00:10:21,100
+nucleus يبقى Lentiform nucleus located between
+
+124
+00:10:22,260 --> 00:10:26,440
+external capsule externally and internal capsule
+
+125
+00:10:26,440 --> 00:10:32,440
+internally or medial okay the lentiform nucleus
+
+126
+00:10:32,440 --> 00:10:41,200
+contains two parts من جزئين
+
+127
+00:10:41,200 --> 00:10:50,410
+واحد بيوتامين اثنينglobus pallidus تتكون من جزءين
+
+128
+00:10:50,410 --> 00:10:57,830
+بيوتامين و globus pallidus البيوتامين بيكون dark
+
+129
+00:10:58,970 --> 00:11:04,690
+Large Lateral Dark Large Lateral طب Globus
+
+130
+00:11:04,690 --> 00:11:09,950
+Ballidas بيكون Smaller Light Medial يعني
+
+131
+00:11:09,950 --> 00:11:15,730
+البيوتامين بيكون لونه غامق و أكبر في الحجم و موجود
+
+132
+00:11:15,730 --> 00:11:20,210
+Lateral و Globus Ballidas بيكون أصغر في الحجم و
+
+133
+00:11:20,210 --> 00:11:25,210
+Light أفتح في اللون و بيكون More Medial نرسم مع
+
+134
+00:11:25,210 --> 00:11:27,490
+بعض الانتفار
+
+135
+00:11:28,770 --> 00:11:35,270
+هذه الـ lentiform nucleus الجزء اللاترال والدارك
+
+136
+00:11:35,270 --> 00:11:41,250
+والكبير اسمه بيوتامين والجزء
+
+137
+00:11:41,250 --> 00:11:45,850
+ال medial و ال light و ال small اسمه globus
+
+138
+00:11:45,850 --> 00:11:50,210
+ballidas يبقى ان هذه هي lentiform nucleus وين
+
+139
+00:11:50,210 --> 00:11:55,550
+موجودة ال lentiform nucleus موجودة بين ال internal
+
+140
+00:11:55,550 --> 00:12:04,600
+capsuleبتكون media اللي لها و external capsule
+
+141
+00:12:06,280 --> 00:12:11,800
+بتكون lateral ليلة يعني هي located between the
+
+142
+00:12:11,800 --> 00:12:16,920
+internal capsule and external capsule ولذلك سميت
+
+143
+00:12:16,920 --> 00:12:21,280
+internal capsule located internal to the lentiform
+
+144
+00:12:21,280 --> 00:12:26,320
+nucleus and external capsule located external to
+
+145
+00:12:26,320 --> 00:12:32,700
+lentiform nucleus okay بدي
+
+146
+00:12:32,700 --> 00:12:37,660
+منكوا الأول حاجة صحتكم من النومتسمعوا لهذه
+
+147
+00:12:37,660 --> 00:12:44,420
+المعادلات هذه التلاتة اول معادلة لينتي فور نوكلياس
+
+148
+00:12:44,420 --> 00:12:49,220
+لينتي فور نوكلياس لينتي فور نوكلياس لينتي فور
+
+149
+00:12:49,220 --> 00:12:53,100
+نوكلياس لينتي
+
+150
+00:12:53,100 --> 00:12:54,780
+فور نوكلياس لينتي فور نوكلياس لينتي فور نوكلياس
+
+151
+00:12:54,780 --> 00:12:55,180
+لينتي فور نوكلياس لينتي فور نوكلياس لينتي فور
+
+152
+00:12:55,180 --> 00:12:55,220
+نوكلياس لينتي فور نوكلياس لينتي فور نوكلياس لينتي
+
+153
+00:12:55,220 --> 00:12:55,300
+فور نوكلياس لينتي فور نوكلياس لينتي فور نوكلياس
+
+154
+00:12:55,300 --> 00:12:55,300
+لينتي فور نوكلياس لينتي فور نوكلياس لينتي فور
+
+155
+00:12:55,300 --> 00:12:55,700
+نوكلياس لينتي فور نوكلياس لينتي فور نوكلياس لينتي
+
+156
+00:12:56,370 --> 00:13:03,250
+Corpus striatum تساوي كودية نوكلياس زائد لينتيفورم
+
+157
+00:13:03,250 --> 00:13:07,090
+طب طالب بدي هتقول بديش أسمي زي هيك بدي أسمي جكر في
+
+158
+00:13:07,090 --> 00:13:15,110
+الدكتوريات أسميها كودية نوكلياس زائد بيوتامين زائد
+
+159
+00:13:15,110 --> 00:13:18,570
+غلوبوس باليداس راح أعتمدلها الإجابة و أضربلها سلام
+
+160
+00:13:19,330 --> 00:13:23,510
+صح ليه؟ لأن ال انتفار من ال نكلياس هي نفسها
+
+161
+00:13:23,510 --> 00:13:28,470
+ابيوتامين زائد غلوباس باليداس بمشي الحال تلاتة
+
+162
+00:13:29,460 --> 00:13:36,260
+نيوسترياتم او استرياتم اللي هي كودات and بيوتامين
+
+163
+00:13:36,260 --> 00:13:40,820
+يعني استرياتم او نيوسترياتم بيكونوا اصغر من كوربوس
+
+164
+00:13:40,820 --> 00:13:45,800
+استرياتم لاحظوا كوربوس استرياتم فيه كودات بيوتامين
+
+165
+00:13:45,800 --> 00:13:49,160
+غلوباس باليداس نيوسترياتم او استرياتم كودات
+
+166
+00:13:49,160 --> 00:13:56,600
+وبيوتامين only without غلوباس باليداسهكذا بيكون
+
+167
+00:13:56,600 --> 00:14:00,400
+انا شرحت لكم هذول اجزاء بتاعة البيزل نوكلياي
+
+168
+00:14:00,400 --> 00:14:09,100
+نشوفهم مع بعض lentiform nucleus هاي ها هاد اسمها
+
+169
+00:14:09,100 --> 00:14:15,040
+lentiform nucleus تتكون من بيوتامين هاي واحد
+
+170
+00:14:15,040 --> 00:14:24,480
+بيوتامين ومن ايش بالازرق من aglobus
+
+171
+00:14:24,480 --> 00:14:33,340
+balidasهذه جلوباس و باليداس وهنا بيوتامن
+
+172
+00:14:33,340 --> 00:14:39,300
+مع بعض بنسميهم لنتي فورم نوكياس لو جمعنا أيه لهم
+
+173
+00:14:40,670 --> 00:14:44,350
+الكودات بالـ tail و بالـ head و بالكله مع بعض
+
+174
+00:14:44,350 --> 00:14:49,010
+بيحدث اسم او بنغير اسم بيصير Corpus striatum
+
+175
+00:14:49,010 --> 00:14:52,810
+Corpus striatum عبارة عن بيوتة من مع الانتفارم او
+
+176
+00:14:52,810 --> 00:14:58,790
+بيوتة من مع عبارة عن كودات مع الانتفارم او بنسمي
+
+177
+00:14:58,790 --> 00:15:03,170
+الكوربوس استرياتم عبارة عن كودات مع الانتفارم او
+
+178
+00:15:03,170 --> 00:15:08,670
+بنسمي كوداتبيوتامين و globus pallidus كله مع بعض
+
+179
+00:15:08,670 --> 00:15:14,710
+هذا يعتبر كوربوس استرياتم طب لو اجينا حذفنا منهم
+
+180
+00:15:14,710 --> 00:15:19,110
+globus
+
+181
+00:15:19,110 --> 00:15:23,970
+pallidus حذفنا منهم ل globus pallidus بسمي ايش
+
+182
+00:15:23,970 --> 00:15:29,070
+بسمي نيوسترياتم نيوسترياتم او استرياتم يعني هو
+
+183
+00:15:29,070 --> 00:15:33,570
+عبارة عن كودة نوكليوس مع بيوتامين only مع بيوتامين
+
+184
+00:15:33,570 --> 00:15:34,370
+okay
+
+185
+00:15:39,800 --> 00:15:45,720
+بنتكلم على classroom classroom it is a thin layer
+
+186
+00:15:45,720 --> 00:15:52,960
+of grey matter وين موجود between the external
+
+187
+00:15:52,960 --> 00:15:58,840
+capsule medially and the white matter of the
+
+188
+00:15:58,840 --> 00:16:02,200
+insula laterally ال function تبعته unknown
+
+189
+00:16:02,200 --> 00:16:06,720
+function بحبوا الطلاب عشان unknown function هى هى
+
+190
+00:16:06,720 --> 00:16:07,140
+classroom
+
+191
+00:16:10,700 --> 00:16:17,620
+هذه هي ال classroom classroom هذه عبارة
+
+192
+00:16:17,620 --> 00:16:22,480
+عن ايه عن part of the basal nuclei وين موجودة؟
+
+193
+00:16:22,480 --> 00:16:28,920
+موجودة بين internal capsule بتكون medial لها ال
+
+194
+00:16:28,920 --> 00:16:31,700
+internal capsule هي white matter بتكون ايش؟ medial
+
+195
+00:16:31,700 --> 00:16:39,020
+و ال white matter of the insulaبتكون lateral هي
+
+196
+00:16:39,020 --> 00:16:43,920
+محصورة بين ال white matter بتاع ال insula و ال
+
+197
+00:16:43,920 --> 00:16:46,480
+external capsule ال external capsule بتكون medial
+
+198
+00:16:46,480 --> 00:16:49,460
+و ال white matter بتاع ال insula بتكون lateral و
+
+199
+00:16:49,460 --> 00:16:52,620
+ال claustrum بتكون موجودة ايه بينهم بتكون ال
+
+200
+00:16:52,620 --> 00:16:59,320
+claustrum موجودة ايه بينهم اخر
+
+201
+00:16:59,320 --> 00:17:03,340
+جزئية في ال basal nuclei اللي هي amygdaloid
+
+202
+00:17:03,340 --> 00:17:07,380
+nucleusاميجدالويد نوكلياس ايش الاميجدالويد نوكلياس
+
+203
+00:17:07,380 --> 00:17:13,520
+it is part of basal nuclei situated in the
+
+204
+00:17:13,520 --> 00:17:17,000
+temporal loop when عاملة connection بتكون
+
+205
+00:17:17,000 --> 00:17:22,020
+connected مع ايه مع ال tail of كودية نوكلياس بتكون
+
+206
+00:17:22,020 --> 00:17:26,790
+موجودة في نهاية ذيلة بتاع الكودية نوكلياسوالها
+
+207
+00:17:26,790 --> 00:17:29,890
+علاقة او جزء من ال limbic system تعتبر جزء من ايه
+
+208
+00:17:29,890 --> 00:17:33,570
+ال limbic system بتاع ال brain what's the function
+
+209
+00:17:33,570 --> 00:17:37,630
+of amygdaloid nucleus زي ما ضربتلكوا على الطاولة
+
+210
+00:17:37,630 --> 00:17:43,620
+ايش صار معاكم بنخاف بيصير معناه ننقذالـ can
+
+211
+00:17:43,620 --> 00:17:46,600
+influence the body response to environmental
+
+212
+00:17:46,600 --> 00:17:50,320
+changes بحالة هذه، أيش بيحدث مع الناس؟ بيزيد ال
+
+213
+00:17:50,320 --> 00:17:52,740
+heart rate، ال blood pressure بيرتفع، ال skin
+
+214
+00:17:52,740 --> 00:17:56,560
+color بيصير شاحب لونه أبيض، بيقلق من ال
+
+215
+00:17:56,560 --> 00:18:00,100
+vasoconstriction و بيرتفع الضغط و بيزيد ال
+
+216
+00:18:00,100 --> 00:18:03,980
+respiratory rate ال amygdaloid نوكلياس، اللي علاقة
+
+217
+00:18:03,980 --> 00:18:07,600
+بالـ ال function of smell
+
+218
+00:18:11,190 --> 00:18:18,590
+هي عندي واضح ال head of كودات هذا ال body بتاع
+
+219
+00:18:18,590 --> 00:18:23,050
+الكودات وهنا ايه ال tail of كودات وفي نهاية ال
+
+220
+00:18:23,050 --> 00:18:27,570
+tail of كودات فى amygdaloid body او اللى هى
+
+221
+00:18:27,570 --> 00:18:29,050
+amygdaloid nucleus
+
+222
+00:18:39,330 --> 00:18:48,190
+لما اجمع هذه الكودات مع
+
+223
+00:18:48,190 --> 00:18:53,610
+ال انتفار مع بعض اسمه كوربوسسترياتم لو جمعنا بس
+
+224
+00:18:53,610 --> 00:18:58,270
+الكودات مع البيوتامين اسمها استرياتم او نيو
+
+225
+00:18:58,270 --> 00:19:01,670
+استرياتم استرياتم او نيو استرياتم
+
+226
+00:19:06,710 --> 00:19:11,770
+أنا شرحت لكم موضوع بيزل نوكلياي بنتاج لجزء الثاني
+
+227
+00:19:11,770 --> 00:19:17,990
+اللي هو الـDiencephalon الـDiencephalon is part of
+
+228
+00:19:17,990 --> 00:19:23,510
+the brain located above the middle brain and
+
+229
+00:19:23,510 --> 00:19:27,330
+between the lower part of two cerebral hemispheres
+
+230
+00:19:27,330 --> 00:19:32,470
+نرسم مع بعض اللي هو الـDiencephalon
+
+231
+00:20:24,040 --> 00:20:36,820
+Okay اللون الأسود هو Diencephalon هذا
+
+232
+00:20:36,820 --> 00:20:43,160
+Interthalamic adhesion هذا هو thalamus هذا
+
+233
+00:20:43,160 --> 00:20:49,870
+subthalamus تحت ال thalamus هذا hypothalamusهذا
+
+234
+00:20:49,870 --> 00:20:55,530
+Metathalamus وهذا Epithalamus هذا الأزرق رسمت أنا
+
+235
+00:20:55,530 --> 00:21:01,430
+الجزء الأزرق هذا أمامي ال brain stem mid brain
+
+236
+00:21:01,430 --> 00:21:07,350
+pons medulla oblongata تحت نكمل اللي هو spinal
+
+237
+00:21:07,350 --> 00:21:13,610
+cord في الأمام هذه هي pituitary gland
+
+238
+00:21:17,470 --> 00:21:23,170
+Gland تتكون من posterior lobe و anterior lobe okay
+
+239
+00:21:23,170 --> 00:21:35,170
+هذا الأسود اللي هو Diencephalon عشان
+
+240
+00:21:35,170 --> 00:21:40,890
+رسمة واضحية نقرب لكم وجهة الموضوع ال Diencephalon
+
+241
+00:21:40,890 --> 00:21:46,830
+ونبسطه في الشرحالـ diencephalon it is part of the
+
+242
+00:21:46,830 --> 00:21:51,890
+brain located above midbrain هي فوق ال midbrain
+
+243
+00:21:51,890 --> 00:21:57,490
+and between the lower part of two cerebral
+
+244
+00:21:57,490 --> 00:22:00,490
+hemisphere وفي الجزء السفلي من ال two cerebral
+
+245
+00:22:00,490 --> 00:22:04,770
+hemisphere embryological origin من ناحية ال
+
+246
+00:22:04,770 --> 00:22:09,470
+embryological origin بيجينا من ال four brainfor
+
+247
+00:22:09,470 --> 00:22:14,970
+brain بيعطينا بروزن سيفالون و بروزن سيفالون بنقسم
+
+248
+00:22:14,970 --> 00:22:20,230
+لقسمين تيلين سيفالون و داين سيفالون تيلين سيفالون
+
+249
+00:22:20,230 --> 00:22:22,690
+بتعطينا الcerebral hemisphere و ال lateral
+
+250
+00:22:22,690 --> 00:22:26,970
+ventricle و الداين سيفالون بيعطينا اللي هو الداين
+
+251
+00:22:26,970 --> 00:22:29,010
+سيفالون نفسه الthalamus, hypothalamus,
+
+252
+00:22:29,070 --> 00:22:31,630
+hypothalamus, subthalamus, metathalamus بيعطينا
+
+253
+00:22:31,630 --> 00:22:35,850
+mammillary bodyش اسمه و فيه بيعطينا كمان ال eyes و
+
+254
+00:22:35,850 --> 00:22:41,880
+بيعطينا a third ventriclecavity of diencephalon is
+
+255
+00:22:41,880 --> 00:22:47,220
+third
+
+256
+00:22:47,220 --> 00:22:51,380
+ventricleهنا بيكون عندى ايه اللي هو ايه ال dying
+
+257
+00:22:51,380 --> 00:22:54,060
+cephalon فى المكان هذا هذا ال dying cephalon
+
+258
+00:22:54,060 --> 00:22:58,280
+بيجينا فى المكان هذا thalamus تحتى subthalamus و
+
+259
+00:22:58,280 --> 00:23:02,740
+فى الأمام هنا بيكون ايه اللى هو ال hypothalamus و
+
+260
+00:23:02,740 --> 00:23:06,900
+أمامه بيكون بتوتر اجلان و اللى فى الخلف بيكون
+
+261
+00:23:06,900 --> 00:23:12,860
+metathalamus و فوقهم بيكون epithalamus what's the
+
+262
+00:23:12,860 --> 00:23:17,520
+subdivisions of dying cephalon؟ من ايش هتكون؟أنا
+
+263
+00:23:17,520 --> 00:23:22,540
+رسمت لكوا يخططوا هاي واضح بتكون من الأب الروحي
+
+264
+00:23:22,540 --> 00:23:26,380
+اللي هو الثلمس أهم واحد فيهم ثلمس بعده
+
+265
+00:23:26,380 --> 00:23:32,700
+hypothalamus subthalamus metathalamus ابثلامس
+
+266
+00:23:32,700 --> 00:23:42,260
+ابثلامس نبدأ بثلمس ايش هو الثلمس؟thalamus عبارة عن
+
+267
+00:23:42,260 --> 00:23:50,600
+large egg shape mass of a grey matter وين موجود؟
+
+268
+00:23:50,600 --> 00:23:55,060
+موجود in the middle of the lower part of two
+
+269
+00:23:55,060 --> 00:23:58,540
+cerebral hemispheres عندنا في right thalamus وفي
+
+270
+00:23:58,540 --> 00:24:03,700
+left thalamus وفي بينهم adhesions هذه ال adhesions
+
+271
+00:24:04,330 --> 00:24:07,230
+بين ال right و ال left بتمر في ال third ventricle
+
+272
+00:24:07,230 --> 00:24:12,370
+اسمها Interthalamic Adhesion Interthalamic ايه؟
+
+273
+00:24:12,370 --> 00:24:19,330
+Adhesion ال site it is located on each side of the
+
+274
+00:24:19,330 --> 00:24:25,670
+third ventricle immediately above subthalamus and
+
+275
+00:24:25,670 --> 00:24:30,010
+medial to posterior limb of internal capsule يعني
+
+276
+00:24:30,010 --> 00:24:36,860
+ال thalamus موجودبيحدوا ال third ventricle من كل
+
+277
+00:24:36,860 --> 00:24:41,780
+جهة يعني عندي right thalamus و left thalamus و في
+
+278
+00:24:41,780 --> 00:24:45,480
+cavity بينهم هذا ال cavity اللي بينهم اسمه ايه؟
+
+279
+00:24:45,480 --> 00:24:49,780
+third ventricle اسمه ايه؟ third ventricle غير هيك
+
+280
+00:24:49,780 --> 00:24:55,160
+ال thalamus بيجي اعلى مباشرة من ال subthalamus فوق
+
+281
+00:24:55,160 --> 00:25:01,190
+ال subthalamus مباشرةو بيكون medial ل .. ل .. ال
+
+282
+00:25:01,190 --> 00:25:06,490
+posterior limb of internal capsule هاي عندي تلمس
+
+283
+00:25:06,490 --> 00:25:11,830
+left
+
+284
+00:25:11,830 --> 00:25:21,030
+تلمس و right تلمس بيحد ال .. third ventricle من
+
+285
+00:25:21,030 --> 00:25:24,290
+الجناب من الجهة ده هانبينهم interthalamic adhesion
+
+286
+00:25:25,160 --> 00:25:32,720
+هذا عبارة عن إيه؟ Interthalamic adhesion وبيكون
+
+287
+00:25:32,720 --> 00:25:37,460
+media
+
+288
+00:25:37,460 --> 00:25:39,580
+للـ posterior limb
+
+289
+00:25:42,040 --> 00:25:46,640
+of internal capsule مي دي للمين للبستيرو ليمب of
+
+290
+00:25:46,640 --> 00:25:50,640
+internal capsule وبيكون ايه above دي subthalamus
+
+291
+00:25:50,640 --> 00:25:55,540
+immediately above لإيه subthalamus هذا هو ال
+
+292
+00:25:55,540 --> 00:25:59,240
+thalamus هذا هو ال thalamus و بينهم في adhesions
+
+293
+00:25:59,240 --> 00:26:03,680
+ال adhesion اسمها interthalamic adhesion واضح عندي
+
+294
+00:26:03,680 --> 00:26:11,040
+على ال axial cut نشرحهم هي عندي thalamus
+
+295
+00:26:13,830 --> 00:26:18,350
+بينهم فيه adhesion اسمها interthalamic adhesions
+
+296
+00:26:18,350 --> 00:26:30,970
+في النصف بيكون موجود cavity of third ventricle و
+
+297
+00:26:30,970 --> 00:26:36,370
+التلمس بيكون موجود هاي ال internal capsule
+
+298
+00:26:36,370 --> 00:26:39,350
+anterior limb posterior limb
+
+299
+00:26:44,640 --> 00:26:52,540
+بيكون وين موجود بيكون Medial لل posterior limb of
+
+300
+00:26:52,540 --> 00:26:57,920
+internal capsule Medial لل posterior limb of
+
+301
+00:26:57,920 --> 00:27:06,180
+internal capsule what's
+
+302
+00:27:06,180 --> 00:27:10,180
+the function of thalamus ايش وظيفة ال thalamus اول
+
+303
+00:27:10,180 --> 00:27:15,690
+شي sensory functionThalamus هو المحطة السنسية
+
+304
+00:27:15,690 --> 00:27:22,110
+العظيمة للبوابة لكل السنسيةto the cerebral cortex
+
+305
+00:27:22,110 --> 00:27:24,970
+except smell, vision, hearing السمل والفيجين
+
+306
+00:27:24,970 --> 00:27:28,890
+والهيرين مابيروحوش على الثلمس السمل مباشرة لل
+
+307
+00:27:28,890 --> 00:27:33,350
+timbral loop والفيجين والهيرين بيروحوا لجزء اسمه
+
+308
+00:27:33,350 --> 00:27:38,290
+metathalamus اسمه ايه؟ metathalamus الفيجين بتروح
+
+309
+00:27:38,290 --> 00:27:41,910
+لشيء اسمه lateral geniculate body والهيرين لشيء
+
+310
+00:27:41,910 --> 00:27:45,110
+اسمه medial geniculate body من ال metathalamus راح
+
+311
+00:27:45,110 --> 00:27:49,590
+نشرحهمأثنين ريجوليشن of consciousness اللي علاقة
+
+312
+00:27:49,590 --> 00:27:53,230
+بالريجوليشن of consciousness ثلاثة emotional
+
+313
+00:27:53,230 --> 00:27:57,510
+regulation اللي علاقة بالريجوليشن والتحكم في
+
+314
+00:27:57,510 --> 00:28:02,810
+العاطفة لو حدث damage لthalamus
+
+315
+00:28:06,640 --> 00:28:11,180
+loss of superficial sensation from the opposite
+
+316
+00:28:11,180 --> 00:28:14,800
+side of the body الإنسان بيفقد الإحساس على ال
+
+317
+00:28:14,800 --> 00:28:20,180
+opposite side ثمين Contralateral sensory attacks
+
+318
+00:28:20,180 --> 00:28:25,680
+يعني بيصير فقدان الإحساس العميق و بيحدث عندهم ترنح
+
+319
+00:28:25,680 --> 00:28:31,540
+حسثلاثة Contralateral Thalamic Pain بيحدث معهم ألم
+
+320
+00:28:31,540 --> 00:28:36,280
+في الجهة المعاكسة Hemipain أربع Emotional
+
+321
+00:28:36,280 --> 00:28:40,580
+Liability مشكلة في العاطفة ممكن يحدث معهم يبكوا
+
+322
+00:28:40,580 --> 00:28:44,840
+بدون سبب يضحكوا بدون سببInvoluntary movement،
+
+323
+00:28:44,840 --> 00:28:50,020
+حركات لا إرادية، حركات لا إرادية، impaired
+
+324
+00:28:50,020 --> 00:28:54,820
+consciousness level، يحدث معهم تدني في درجة الوعي
+
+325
+00:28:57,550 --> 00:29:01,490
+أشرح ال ابي ثلامث ان شاء الله جزئية هذه و أكتفي من
+
+326
+00:29:01,490 --> 00:29:04,690
+الجزء الأول في المحاضرة و بعدها ننتقل للجزء الثاني
+
+327
+00:29:04,690 --> 00:29:09,370
+من المحاضرة ال ابي ثلامث أخر جزء راح يكون هو عبارة
+
+328
+00:29:09,370 --> 00:29:13,390
+عن part of the diencephalon هو جزء من ال
+
+329
+00:29:13,390 --> 00:29:17,270
+diencephalon when attached, attached على ال
+
+330
+00:29:17,270 --> 00:29:22,250
+posterior end of upper surface of ثلامث زي ما احنا
+
+331
+00:29:22,250 --> 00:29:26,740
+شايفينه هذا posterior end هذا الخلفيهذا الـ
+
+332
+00:29:26,740 --> 00:29:30,620
+posterior وهذا
+
+333
+00:29:30,620 --> 00:29:40,240
+الـ anterior إيه بقى ال .. ال apthalamus موجود
+
+334
+00:29:40,240 --> 00:29:44,860
+وين؟ على ال posterior end of the upper surface of
+
+335
+00:29:44,860 --> 00:29:50,560
+thalamus بتكون من right and left habanular nuclei
+
+336
+00:29:50,560 --> 00:29:54,960
+habanular commissionerPosterior commissure و
+
+337
+00:29:54,960 --> 00:29:58,900
+Penial body أو Penial gland اللي بتفرز مادة
+
+338
+00:29:58,900 --> 00:30:02,480
+الميلاتانين اللي بيحمي الجسم من ال cancer و
+
+339
+00:30:02,480 --> 00:30:06,540
+السرطانات عشان هيك الإنسان لما ينام حاول يفصل
+
+340
+00:30:06,540 --> 00:30:10,760
+الإضاءة لأنه بتشتغل في الظلام هذه الغداء و بتفرز
+
+341
+00:30:10,760 --> 00:30:13,620
+مادة الميلاتانين بيحمينا الجسم من الخلايا
+
+342
+00:30:13,620 --> 00:30:19,660
+السرطانية هنا واضح اللي هو ال Penial
+
+343
+00:30:22,480 --> 00:30:27,060
+body أو بين ال gland فوقه من و فوق شوية بتكون اللي
+
+344
+00:30:27,060 --> 00:30:30,120
+هو الhabinolar commissar من و تحت شوية بتكون عندنا
+
+345
+00:30:30,120 --> 00:30:34,680
+اللي هي ال posterior commissar و الhabinolar
+
+346
+00:30:34,680 --> 00:30:39,720
+nuclei بيكونوا موجودين داخل ال habinolar commissar
+
+347
+00:30:39,720 --> 00:30:45,620
+مش واضحين هنقلهم علاقة ب other functions و بيعملوا
+
+348
+00:30:45,620 --> 00:30:49,430
+connection between ال bothCerebral hemispheres
+
+349
+00:30:49,430 --> 00:30:52,350
+بيعملوا رابط ما بين ال right و ال left cerebral
+
+350
+00:30:52,350 --> 00:30:57,590
+hemisphere الجزئية الأولى من المحاضرة نكتفي فيها و
+
+351
+00:30:57,590 --> 00:31:01,130
+ان شاء الله لنا لقاء في الجزء الآخر فرح نكمل فيه
+
+352
+00:31:01,130 --> 00:31:05,350
+اللي هو ال dying cephalon و نتكلم بالتفصيل على ال
+
+353
+00:31:05,350 --> 00:31:09,730
+anatomy and physiology of cerebellar دومتم بخير
+
+354
+00:31:09,730 --> 00:31:10,630
+يعطيكم ألف عام
+
diff --git a/PL9fwy3NUQKwYBFfMQ7WkgiguUA_8aXeXd/neIW9sKmQGo.srt b/PL9fwy3NUQKwYBFfMQ7WkgiguUA_8aXeXd/neIW9sKmQGo.srt
new file mode 100644
index 0000000000000000000000000000000000000000..cf8cb692711777033496078d07d7692d5e26066b
--- /dev/null
+++ b/PL9fwy3NUQKwYBFfMQ7WkgiguUA_8aXeXd/neIW9sKmQGo.srt
@@ -0,0 +1,1010 @@
+1
+00:00:05,030 --> 00:00:09,290
+بسم الله الرحمن الرحيم نكمل الجزء الثاني من محاضرة
+
+2
+00:00:09,290 --> 00:00:13,050
+autonomic nervous system احنا في الجزء الأول شرحنا 
+
+3
+00:00:13,050 --> 00:00:16,910
+لكم الـ autonomic nervous system شرحنا الـ first
+
+4
+00:00:16,910 --> 00:00:20,270
+order neuron second order neuron شرحنا الـ origin
+
+5
+00:00:20,270 --> 00:00:23,070
+of the autonomic nervous system origin of
+
+6
+00:00:23,070 --> 00:00:26,990
+sympathetic and parasympathetic واطرقنا إلى الجزء
+
+7
+00:00:26,990 --> 00:00:30,580
+الأول من الـ parasympathetic nervous system شرحنا
+
+8
+00:00:30,580 --> 00:00:35,060
+لكم الـ part of the parasympathetic nervous system
+
+9
+00:00:35,060 --> 00:00:40,500
+اللي هو الجزء الثاني من الـ parasympathetic اللي هو
+
+10
+00:00:40,500 --> 00:00:46,200
+الـ part of the parasympathetic nervous system
+
+11
+00:00:46,940 --> 00:00:50,840
+تفاجأنا أن هذا موجود في S2, S3, S4 segment of
+
+12
+00:00:50,840 --> 00:00:54,200
+spinal cord يبقى الـ first order neuron بتاعة الـ
+
+13
+00:00:54,200 --> 00:00:58,340
+sacral part of parasympathetic nervous system
+
+14
+00:00:58,340 --> 00:01:02,120
+موجودين في الـ anterior horn cells
+
+15
+00:01:08,600 --> 00:01:14,200
+وظيفتهم 
+
+16
+00:01:14,200 --> 00:01:17,960
+وظيفتهم 
+
+17
+00:01:17,960 --> 00:01:20,400
+وظيفتهم 
+
+18
+00:01:20,400 --> 00:01:22,880
+وظيفتهم
+
+19
+00:01:22,880 --> 00:01:28,610
+وظيفتهم genital organs يبقى أن الـ sacral أو pelvic
+
+20
+00:01:28,610 --> 00:01:32,850
+parasympathetic nervous system مسئول عن الـ
+
+21
+00:01:32,850 --> 00:01:37,010
+emptying of urinary bladder and rectum مسئول عن
+
+22
+00:01:37,010 --> 00:01:42,430
+عملية الـ defecation و الـ urination أنتم شايفين
+
+23
+00:01:42,430 --> 00:01:50,990
+هنا الـ origin تبعهم من S2 S3 S4 part of a sacral
+
+24
+00:01:50,990 --> 00:01:55,140
+nervous system اللي هو تابِع الـ Parasympathetic و
+
+25
+00:01:55,140 --> 00:01:59,960
+supply genital organs و بياخد الـ left one third of
+
+26
+00:01:59,960 --> 00:02:05,260
+a transverse colon, descending colon و الـ rectum
+
+27
+00:02:05,260 --> 00:02:11,340
+كل هذا بياخد من وين؟ من الـ ciliary part أن راجع الـ
+
+28
+00:02:11,340 --> 00:02:14,840
+Parasympathetic ganglia in the head and neck
+
+29
+00:02:14,840 --> 00:02:19,840
+لاحظوا الـ ciliary ganglion شرحناها في البداية
+
+30
+00:02:21,710 --> 00:02:26,590
+النيرون الأول هو Edinger Westphal nucleus النيرون
+
+31
+00:02:26,590 --> 00:02:29,110
+الثاني هو ciliary ganglion preganglionic fibers
+
+32
+00:02:29,110 --> 00:02:34,090
+الـ parasympathetic root اللي هو يعتبر
+
+33
+00:02:34,090 --> 00:02:39,150
+oculomotor nerve الـ target organ هو الـ sphincter pupillae
+
+34
+00:02:39,150 --> 00:02:43,070
+و مصدر الـ ciliary أنا براجع cranial part of parasympathetic nervous
+
+35
+00:02:43,070 --> 00:02:47,380
+system ينتقل لـ Superior Salivary
+
+36
+00:02:47,380 --> 00:02:51,560
+Nucleus superior salivary nucleus اللي هي first
+
+37
+00:02:51,560 --> 00:02:57,820
+order neuron للـ facial nerve لها 2 جانجليا sphenopalatine أو
+
+38
+00:02:57,820 --> 00:03:05,210
+pterygoid palatine ganglion بتعطينا fibers للنيرف  greater superficial petrosal nerve
+
+39
+00:03:05,210 --> 00:03:09,910
+اللي جاي من الـ facial nerve supplies
+
+40
+00:03:09,910 --> 00:03:14,830
+اللي هي lacrimal gland, glands of nose, mouth and
+
+41
+00:03:14,830 --> 00:03:21,160
+pharynx والـ pterygopalatine ganglion بتعطينا
+
+42
+00:03:21,160 --> 00:03:26,100
+fibers لـ .. بتاخد fibers اللي هي parasympathetic root
+
+43
+00:03:26,100 --> 00:03:30,300
+بتاخد من وين؟ من الـ facial nerve اللي بيغذي الـ
+
+44
+00:03:30,300 --> 00:03:35,250
+submandibular و sublingual salivary gland Inferior
+
+45
+00:03:35,250 --> 00:03:40,670
+Salivary nucleus مسئولة عن Glossopharyngeal nerve
+
+46
+00:03:40,670 --> 00:03:45,290
+تعطينا preganglionic fibers للأوتيك جانجليون من
+
+47
+00:03:45,290 --> 00:03:50,790
+بياخد منها الـ fibers بياخد الـ lesser superficial
+
+48
+00:03:50,790 --> 00:03:53,970
+petrosal nerve بيسلم للأوتيك جانجليون و الأوتيك
+
+49
+00:03:53,970 --> 00:03:58,670
+ganglion اللي هو بتسلم للـ Parotid gland من وين هو
+
+50
+00:03:58,670 --> 00:04:00,610
+ليه أصبح superficial petrosal nerve؟ أول مرة
+
+51
+00:04:00,610 --> 00:04:03,470
+تسمعوه هو جاي من الـ Glossopharyngeal as a branch
+
+52
+00:04:03,470 --> 00:04:08,950
+of Glossopharyngeal nerve الـ dorsal motor nucleus
+
+53
+00:04:08,950 --> 00:04:14,550
+of vagus nerve بتعطينا لماني جانجليا قلنا عشرات in
+
+54
+00:04:14,550 --> 00:04:19,350
+different organs للـ preganglionic fibers بتاعها اللي هو
+
+55
+00:04:19,350 --> 00:04:23,970
+vagus nerve and its branches supply heart
+
+56
+00:04:23,970 --> 00:04:29,680
+respiratory system and abdominal viscera هيلاحظوا
+
+57
+00:04:29,680 --> 00:04:33,640
+الـ oculomotor nerve ايش اسم الـ first order neuron
+
+58
+00:04:33,640 --> 00:04:36,660
+تبعته Edinger Westphal nucleus عن طريق الـ
+
+59
+00:04:36,660 --> 00:04:41,380
+oculomotor nerve أدنى inferior division أدنى nerve to
+
+60
+00:04:41,380 --> 00:04:45,200
+inferior oblique muscle بتغذي الـ ciliary muscle آسف
+
+61
+00:04:45,200 --> 00:04:48,260
+بتغذي الـ ciliary ganglion الـ ciliary ganglion اللي
+
+62
+00:04:48,260 --> 00:04:51,920
+هي second order neuron بتغذي الـ ciliary muscle و الـ
+
+63
+00:04:51,920 --> 00:04:54,020
+sphincter pupillae muscle أنا قاعد براجع المراجعة
+
+64
+00:04:54,020 --> 00:04:58,090
+اللي شرحتها نجي للـ facial nerve الـ nucleus first
+
+65
+00:04:58,090 --> 00:05:02,210
+order neuron اسمه superior salivary nucleus هذي
+
+66
+00:05:02,210 --> 00:05:05,450
+بتعطينا two branches Parasympathetic greater
+
+67
+00:05:05,450 --> 00:05:09,130
+superficial petrosal nerve بيروح لـ sphenopalatine و pterygoid palatine
+
+68
+00:05:09,130 --> 00:05:14,230
+ganglion و بيغذي لنا الـ glands و glands of nose
+
+69
+00:05:14,230 --> 00:05:20,400
+pharynx palate و في جزء ثاني منها بيروح عن طريق
+
+70
+00:05:20,400 --> 00:05:24,920
+chordatympani nerve submandibular ganglion و الـ
+
+71
+00:05:24,920 --> 00:05:28,020
+submandibular ganglion بتعطينا postganglionic
+
+72
+00:05:28,020 --> 00:05:32,400
+fibers للـ target organs اللي هم sublingual and
+
+73
+00:05:32,400 --> 00:05:36,860
+submandibular glands نجي لـ glossopharyngeal nerve الـ
+
+74
+00:05:36,860 --> 00:05:40,020
+first order neuron بتاعها inferior salivary nucleus
+
+75
+00:05:40,020 --> 00:05:44,210
+الـ fibers ساعتها عن طريق glossopharyngeal nerve عن طريق
+
+76
+00:05:44,210 --> 00:05:47,570
+الـ lesser superficial petrosal nerve بتروح للـ otic
+
+77
+00:05:47,570 --> 00:05:50,270
+ganglion يبقى هذه الـ fibers كلها تعتبر pre
+
+78
+00:05:50,270 --> 00:05:53,690
+ganglionic fibers تروح للـ otic ganglion أو
+
+79
+00:05:53,690 --> 00:05:56,650
+الـ otic ganglion اللي هو second order neuron الـ
+
+80
+00:05:56,650 --> 00:06:01,430
+postganglionic fibers بيسلمها لمين؟ لـ parotid gland
+
+81
+00:06:02,320 --> 00:06:06,080
+هيك بكون خلصت الموضوع بقرا sympathetic nervous
+
+82
+00:06:06,080 --> 00:06:10,400
+system الآن بنتقل للـ sympathetic nervous system الـ
+
+83
+00:06:10,400 --> 00:06:12,700
+origin تبعها sympathetic nervous system
+
+84
+00:06:12,700 --> 00:06:18,020
+thoracolumbar من T1 لـ L2 يبقى sympathetic nervous
+
+85
+00:06:18,020 --> 00:06:21,900
+system اللي علاقة بالـ intermedio lateral nuclei
+
+86
+00:06:21,900 --> 00:06:27,340
+اللي موجودة في الـ lateral horn cells من T1 لحد L2
+
+87
+00:06:27,340 --> 00:06:34,240
+في بعض المراجع بيقولوا من C8 من C8 لحد ايش الاثنين
+
+88
+00:06:34,240 --> 00:06:41,760
+ما علينا لكن أكثر شيء نعتمد T1 L2 part لذلك سميت
+
+89
+00:06:41,760 --> 00:06:46,960
+thoracolumbar nervous system هذه هي الـ sympathetic
+
+90
+00:06:46,960 --> 00:06:50,020
+nervous system sympathetic nervous system بتبدأ من
+
+91
+00:06:50,020 --> 00:06:52,120
+T1 لحد L2
+
+92
+00:06:54,750 --> 00:06:57,770
+Sympathetic Ganglia، فيه نوعين من الـ Sympathetic
+
+93
+00:06:57,770 --> 00:07:01,970
+Ganglia، فيه عبارة Vertebral Ganglia، بتكونها
+
+94
+00:07:01,970 --> 00:07:06,930
+sympathetic chain حوالين الـ vertebral column وعددهم
+
+95
+00:07:06,930 --> 00:07:10,370
+من كل جهة 22 لـ 23 جانجيليا
+
+96
+00:07:10,370 --> 00:07:14,870
+بيكونوا على الـ right و على left side من الـ spine
+
+97
+00:07:14,870 --> 00:07:18,330
+وفي collateral جانجيليا بعيدة شوية اسمها
+
+98
+00:07:18,330 --> 00:07:21,570
+peripheral جانجيليا بتكون في الـ cavity around
+
+99
+00:07:21,570 --> 00:07:27,790
+large arteries زي celiac superior و inferior
+
+100
+00:07:27,790 --> 00:07:33,510
+mesenteric ganglia How many sympathetic ganglia
+
+101
+00:07:33,510 --> 00:07:37,750
+are found in the sympathetic chain؟ قلنا من كل جهة
+
+102
+00:07:37,750 --> 00:07:42,230
+فيه 22 إلى 23 ganglia، cervical بيكون فيه ganglia
+
+103
+00:07:42,230 --> 00:07:44,190
+ثلاثة، هذول موجودين في الـ peripheral nervous
+
+104
+00:07:44,190 --> 00:07:48,610
+system، thoracic عشر إلى اثنا عشر، lumbar أربع إلى
+
+105
+00:07:48,610 --> 00:07:52,270
+خمسة، sacral أربع إلى خمسة، أنا بتكلم على pairs،
+
+106
+00:07:52,270 --> 00:07:55,960
+زوج، على الـ right و على الـ left right و left زوج الـ
+
+107
+00:07:55,960 --> 00:08:00,500
+ganglia بتكون واحدة اسمها stellate ganglion stellate
+
+108
+00:08:00,500 --> 00:08:06,520
+ganglion لو جمعناهم بنجد أن من كل جهة فيه 22 لـ 23
+
+109
+00:08:06,520 --> 00:08:12,320
+جانجليون هذا هو sympathetic chain زي العقد بيكون
+
+110
+00:08:12,320 --> 00:08:15,080
+حوالين الـ vertebral column من الـ right و من الـ
+
+111
+00:08:15,080 --> 00:08:19,540
+left هذا اسمه sympathetic chain وهذه هي الـ ganglia
+
+112
+00:08:19,540 --> 00:08:23,220
+هذه thoracic ganglia على سبيل المثال فوق بنطلع على
+
+113
+00:08:23,220 --> 00:08:27,700
+الـ cervical ganglia هاي واضح عندي cervical ganglia
+
+114
+00:08:27,700 --> 00:08:33,500
+superior هاي middle وهاي inferior نفس
+
+115
+00:08:33,500 --> 00:08:37,440
+الشيء هذه تعتبر sympathetic chain sympathetic ايه؟
+
+116
+00:08:37,440 --> 00:08:42,660
+chain الـ cervical sympathetic ganglia قلنا ثلاثة
+
+117
+00:08:42,660 --> 00:08:46,060
+في superior cervical sympathetic ganglion وفي
+
+118
+00:08:46,060 --> 00:08:49,380
+middle وفي inferior أنا بكتفي فيهم لأن في
+
+119
+00:08:49,380 --> 00:08:52,420
+الطب أنا بشرحهم بشرح الـ branches بتاعتهم كلهم كل
+
+120
+00:08:52,420 --> 00:08:56,480
+ganglion ايش بتخرج أعصاب منها وايه بتغذي هاي عندي
+
+121
+00:08:56,480 --> 00:09:01,100
+اللي هو superior cervical ganglion وهادي middle
+
+122
+00:09:01,100 --> 00:09:05,400
+cervical ganglion وهادي inferior cervical ganglion
+
+123
+00:09:05,400 --> 00:09:11,000
+الـ inferior cervical ganglion هذه بتتحد مع الـ first
+
+124
+00:09:11,000 --> 00:09:14,820
+thoracic و بيسموها cervicothoracic ganglion أو
+
+125
+00:09:14,820 --> 00:09:18,840
+stellate 
+
+126
+00:09:18,840 --> 00:09:20,400
+ganglion
+
+127
+00:09:27,170 --> 00:09:31,390
+Middle Cervical Sympathetic Ganglion و Inferior
+
+128
+00:09:31,390 --> 00:09:35,110
+Cervical Sympathetic Ganglion الـ inferior بتسميها
+
+129
+00:09:35,110 --> 00:09:38,850
+cervicothoracic لأنها بتتحد مع الـ first thoracic
+
+130
+00:09:38,850 --> 00:09:42,650
+ganglion وبيسميها stellate ganglion اللي هي اسم
+
+131
+00:09:42,650 --> 00:09:51,150
+ثاني ايه؟ stellate ganglion okay
+
+132
+00:09:51,150 --> 00:09:57,500
+لأن نرجع الـ effect بنتكلم الآن على physiology pure
+
+133
+00:09:57,500 --> 00:10:01,460
+physiology ايش دور الـ sympathetic nervous system و
+
+134
+00:10:01,460 --> 00:10:06,220
+ايش دور الـ parasympathetic nervous system على الـ
+
+135
+00:10:06,220 --> 00:10:13,920
+organs لو أخدنا الـ eye الـ pupils sympathetic
+
+136
+00:10:13,920 --> 00:10:19,840
+nervous system بتعمل dilation بتغذي عضلة اسمها
+
+137
+00:10:19,840 --> 00:10:24,140
+dilator pupillae muscle بتوسع حدقة العين الـ
+
+138
+00:10:24,140 --> 00:10:28,520
+parasympathetic لأ بتغذي constrictor pupillae muscle
+
+139
+00:10:28,520 --> 00:10:31,600
+عن طريق العصب الثالث الـ oculomotor nerve و غضبتها
+
+140
+00:10:31,600 --> 00:10:35,500
+بتعمل constriction بتعمل miosis، even هذي بتعمل
+
+141
+00:10:35,500 --> 00:10:41,380
+mydriasis يعني توسيع حدقة العين و الـ
+
+142
+00:10:41,380 --> 00:10:47,060
+parasympathetic بتعمل miosis نجي على الـ ciliary
+
+143
+00:10:47,060 --> 00:10:51,880
+muscle اللي علاقة بالـ accommodation أنا راح أشرحها
+
+144
+00:10:51,880 --> 00:10:58,480
+في محاضرة الـ optic nerve في special sensation الـ
+
+145
+00:10:58,480 --> 00:11:02,220
+sympathetic بتعمل لنا relaxation و الـ
+
+146
+00:11:02,220 --> 00:11:06,800
+parasympathetic contraction الآن نتكلم على glands
+
+147
+00:11:06,800 --> 00:11:13,540
+اللي علاقة بالـ glands و nasopharyngeal glands تتذكروا
+
+148
+00:11:13,540 --> 00:11:17,400
+لما اتقطعت بصل ايش بيصير معكم sympathetic بتعمل
+
+149
+00:11:17,400 --> 00:11:20,580
+reduction بتقلل الـ secretion لأن هي بتعمل
+
+150
+00:11:20,580 --> 00:11:24,100
+vasoconstriction الـ parasympathetic بيزود الـ
+
+151
+00:11:24,100 --> 00:11:28,700
+secretion increase لأن مين بيغذي الـ nasopharyngeal
+
+152
+00:11:28,700 --> 00:11:31,960
+glands و الـ lacrimal gland بيغذيها اللي هو
+
+153
+00:11:31,960 --> 00:11:35,360
+sphenopalatine ganglion أو عن طريق الـ greater
+
+154
+00:11:35,360 --> 00:11:38,900
+superficial petrosal nerve اللي أصله جاي من facial
+
+155
+00:11:38,900 --> 00:11:45,060
+nerve salivary gland مثل submandibular sublingual
+
+156
+00:11:45,060 --> 00:11:49,980
+كمان نفس الشيء الـ parasympathetic هنا راح تعمل
+
+157
+00:11:49,980 --> 00:11:53,740
+increase للـ secretion الـ sympathetic بتعمل
+
+158
+00:11:53,740 --> 00:11:58,240
+vasoconstriction و reduce secretion نيجي لقى الـ
+
+159
+00:11:58,240 --> 00:12:03,700
+heart على القلب sympathetic nervous system بتزود الـ
+
+160
+00:12:03,700 --> 00:12:09,240
+heart rate و بتزود الـ muscle contraction لو كنا
+
+161
+00:12:09,240 --> 00:12:15,480
+ماشيين في الظلام فجأة نبح عليك كلب أو حدث انفجار
+
+162
+00:12:15,480 --> 00:12:20,160
+بتجري ما بتدري وين تروح و الـ heart rate تبعك ايه
+
+163
+00:12:20,160 --> 00:12:23,260
+بيشتغل بسرعة لأن اشتغل الجهاز السمبثاوي الـ
+
+164
+00:12:23,260 --> 00:12:25,880
+sympathetic nervous system اشتغلت لكن على الـ
+
+165
+00:12:25,880 --> 00:12:29,140
+parasympathetic nervous system الـ parasympathetic
+
+166
+00:12:29,140 --> 00:12:35,400
+بتقلل الـ heart rate و بتقلل الـ muscle contraction lung
+
+167
+00:12:37,330 --> 00:12:42,510
+على الـ muscles of bronchi bronchial muscles الـ
+
+168
+00:12:42,510 --> 00:12:45,530
+sympathetic nervous system تعمل dilation للقصبة
+
+169
+00:12:45,530 --> 00:12:49,090
+والـ parasympathetic تعمل constriction للقصبة
+
+170
+00:12:49,090 --> 00:12:52,650
+عشان هيك الناس اللي عندهم أزمة بنعطيهم علاج اللي
+
+171
+00:12:52,650 --> 00:12:55,310
+علاقة بالـ sympathetic nervous system بيزود الـ
+
+172
+00:12:55,310 --> 00:12:59,070
+sympathetic nervous system عشان يعمل توسيع وارتخاء
+
+173
+00:12:59,070 --> 00:13:03,030
+للعضلات للـ smooth muscles بتاعة القصبة
+
+174
+00:13:03,030 --> 00:13:09,430
+الهوائية وشعب الهوائية لكن على الـ blood vessels على
+
+175
+00:13:09,430 --> 00:13:12,150
+الـ arteries الموجودين الـ sympathetic بتعمل
+
+176
+00:13:12,150 --> 00:13:15,410
+constriction و الـ parasympathetic بتعمل dilatation
+
+177
+00:13:15,410 --> 00:13:22,630
+ننتقل لـ G I system على الـ G I tract أو G I system
+
+178
+00:13:22,630 --> 00:13:28,630
+الـ muscles of wall الـ sympathetic بتقلل الـ peristaltic،
+
+179
+00:13:28,630 --> 00:13:32,230
+بتقلل حركة الأمعاء الـ parasympathetic بتزود حركة
+
+180
+00:13:32,230 --> 00:13:35,930
+الأمعاء، بتعمل contractions للـ sphincters
+
+181
+00:13:35,930 --> 00:13:41,510
+الـ sympathetic بتعمل spasm، constriction الـ para
+
+182
+00:13:41,510 --> 00:13:45,720
+sympathetic بتعمل relaxation للـ glands الـ
+
+183
+00:13:45,720 --> 00:13:48,980
+sympathetic بتقلل الإفراز الغدد، عصارة المعدة أو
+
+184
+00:13:48,980 --> 00:13:52,620
+عصارة الأمعاء لكن الـ parasympathetic بتزود، بتعمل
+
+185
+00:13:52,620 --> 00:13:57,560
+increase للـ secretion الـ kidney للـ kidney الـ
+
+186
+00:13:57,560 --> 00:14:00,940
+sympathetic nervous system بتعمل decrease للـ
+
+187
+00:14:00,940 --> 00:14:04,68
+
+201
+00:14:54,930 --> 00:15:03,470
+Bladder الـ
+
+202
+00:15:03,470 --> 00:15:08,850
+Sympathetic بتعمل Relaxation و Spasm للسفنغتر ال
+
+203
+00:15:08,850 --> 00:15:11,290
+Parasympathetic بتعملها Contraction و Relaxation
+
+204
+00:15:11,290 --> 00:15:17,130
+للسفنغتر عشان تخرج الـ Bile للددنم و يساعد في عملية
+
+205
+00:15:17,130 --> 00:15:22,350
+الهضم على الـ Liver الـ Sympathetic بتعمل Increase
+
+206
+00:15:22,350 --> 00:15:25,990
+للـ Conversion of Glycogen لجليكوز يبقى في ال
+
+207
+00:15:25,990 --> 00:15:30,280
+Sympathetic بيقولك والله زعل و سكر و ارتفع السكر و
+
+208
+00:15:30,280 --> 00:15:32,900
+السكر ارتفع ليش زعل و السكر ارتفع؟ لأن الـ
+
+209
+00:15:32,900 --> 00:15:34,960
+Sympathetic Nervous System بزيد مع الـ Stress و
+
+210
+00:15:34,960 --> 00:15:40,200
+التوتر وبتحول جليكوجين لجلوكوز وبترفع السكر في
+
+211
+00:15:40,200 --> 00:15:46,180
+الدم البارا Sympathetic بـ Stimulation للإنسولين و
+
+212
+00:15:46,180 --> 00:15:50,580
+Glycogen Secretion في الحالة هذه هي البارا 
+
+213
+00:15:50,580 --> 00:15:54,980
+Sympathetic راح تزيد الإنسولين و تدخل الجلوكوز في
+
+214
+00:15:54,980 --> 00:16:00,510
+الخلايا Intracellular على الـ Pancreas Sympathetic
+
+215
+00:16:00,510 --> 00:16:06,490
+تعمل Inhibition للإنسولين و بتقلل الإنسولين و بتزود ال
+
+216
+00:16:06,490 --> 00:16:12,010
+Glycogen Secretion لكن بارا سيمباتاتيك تعمل
+
+217
+00:16:12,010 --> 00:16:16,850
+Stimulation للإنسولين و لجليكوجين Secretion هذا من
+
+218
+00:16:16,850 --> 00:16:21,270
+ناحية البانكرياس الـ Liver هنا أنا قلتلكوا فاطية
+
+219
+00:16:21,270 --> 00:16:25,610
+الليفر على الـ Liver الـ Sympathetic بتزود قولنا ال
+
+220
+00:16:25,610 --> 00:16:30,970
+Conversion بتاع الـ Glycogen لجلوكوز لكن ال
+
+221
+00:16:30,970 --> 00:16:34,970
+Parasympathetic راح تزود الـ Release of Bile راح
+
+222
+00:16:34,970 --> 00:16:39,810
+تزود إفراز الغدة تبع المرارة و العصارة بتاعة
+
+223
+00:16:39,810 --> 00:16:44,890
+المرارة لعملية الهضم على الـ Male Sex الـ Sympathetic
+
+224
+00:16:44,890 --> 00:16:50,450
+بتزود عملية الـ Ejaculation و الـ Parasympathetic
+
+225
+00:16:50,450 --> 00:16:53,390
+بتزود عملية الـ Erection الحدث اللي علاقة بال
+
+226
+00:16:53,390 --> 00:16:55,070
+Sympathetic و الانتصاب اللي علاقة بال
+
+227
+00:16:55,070 --> 00:16:58,830
+Parasympathetic على الـ Pineal Gland موجودة في الدماغ
+
+228
+00:16:58,830 --> 00:17:03,450
+الـ Sympathetic اللي علاقة بالـ Increase ميلاتونين و
+
+229
+00:17:03,450 --> 00:17:05,950
+الـ Synthesis و الـ Secretion بتاعه ال
+
+230
+00:17:05,950 --> 00:17:10,530
+Parasympathetic مالهاش أي دور على الـ Skin، Sweaty
+
+231
+00:17:10,530 --> 00:17:13,350
+Glands، لما أنا بعصب، بتوتر، بتلاقي إيش بيصير
+
+232
+00:17:13,350 --> 00:17:17,150
+معانا، بنعرق، Sympathetic بتزود الـ Secretion بتاع
+
+233
+00:17:17,150 --> 00:17:21,250
+الـ Sweat، و بقية Sympathetic مالهاش دور، في عضلة
+
+234
+00:17:21,250 --> 00:17:25,710
+صغيرة اسمها Pilomotor Muscle، هاد إلها علاقة
+
+235
+00:17:25,710 --> 00:17:29,550
+بانتصاب الشعر، الحاجة جسمته حاجة كويسة بتقول،
+
+236
+00:17:29,550 --> 00:17:33,720
+شفتلكوا هالمنظر، شعري ويقف والله الحاجة بتعرف في
+
+237
+00:17:33,720 --> 00:17:36,980
+Sympathetic Nervous System شعارها ويقف ليه؟ لأن
+
+238
+00:17:36,980 --> 00:17:40,080
+Sympathetic Nervous System بيعمل Contraction
+
+239
+00:17:40,080 --> 00:17:45,820
+للـ Pilum Motor Muscle هذي بتوقف الشعر هو لو شاف 
+
+240
+00:17:45,820 --> 00:17:49,140
+المنظر و شعريه ويقف والله كلام هو مظبوط الحاجة ال
+
+241
+00:17:49,140 --> 00:17:53,680
+Platelet صفائح الدموية Sympathetic بتعمل Increase
+
+242
+00:17:53,680 --> 00:17:57,260
+للـ Aggregation الصفائح الدموية بتزود إيش التصاق
+
+243
+00:17:57,260 --> 00:18:00,700
+الصفائح الدموية Sympathetic Nervous System بتزود
+
+244
+00:18:00,700 --> 00:18:04,600
+التصاق الصفائح الدموية و بيزود عملية التجلط عشان
+
+245
+00:18:04,600 --> 00:18:07,820
+هيك بيقولك والله زعل و كان عنده Stress و توتر و
+
+246
+00:18:07,820 --> 00:18:10,960
+حدث معاه جلطة دماغية أو جلطة في القلب ليه؟ لأن
+
+247
+00:18:10,960 --> 00:18:13,860
+Sympathetic Nervous System بيزود التصاق السفائح
+
+248
+00:18:13,860 --> 00:18:17,360
+الدموية بارا Sympathetic مالهاش دور الـ Lipolysis
+
+249
+00:18:17,360 --> 00:18:22,940
+Sympathetic بيزود الـ Lipolysis الحرق الدهون ال
+
+250
+00:18:22,940 --> 00:18:27,880
+Renin Secretion Sympathetic بيزود إفراز الرنين و زي
+
+251
+00:18:27,880 --> 00:18:32,560
+ما أنتم عارفين الرنين بتحول لـ أنجوتنزين و بيزود
+
+252
+00:18:32,560 --> 00:18:36,320
+الضغط و بيرتفع عندهم ضغط الدم عشان هيك بيقولك
+
+253
+00:18:36,320 --> 00:18:39,500
+والله مع الذعل اتعرض لصدمة نفسية و ذعل و الضغط
+
+254
+00:18:39,500 --> 00:18:44,250
+ارتفع و أصلاً عنده ضغط Okay وآخر شيء اللي هو Muscles
+
+255
+00:18:44,250 --> 00:18:50,710
+اللي بتفرز الـ Histamine الـ Sympathetic Nervous System
+
+256
+00:18:50,710 --> 00:18:53,590
+بتعمل Decrease للـ Effect تبعها و للـ Degradation و
+
+257
+00:18:53,590 --> 00:18:56,810
+بتقلل إفراز الـ Histamine لذلك بنستخدم في ال
+
+258
+00:18:56,810 --> 00:18:58,830
+Anaphylactic Shock و في الـ Allergy .. Severe
+
+259
+00:18:58,830 --> 00:19:01,170
+Allergy و مع Anaphylactic Shock و بيستخدموا
+
+260
+00:19:01,170 --> 00:19:04,010
+Adrenaline تابع للـ Sympathetic Nervous System
+
+261
+00:19:04,010 --> 00:19:08,660
+بيقلل الـ Degradation of Muscle Cells وبقلل إفراز
+
+262
+00:19:08,660 --> 00:19:11,980
+الـ Histamine اللي علاقة بالحساسية الـ بارا سيمباتاتيك
+
+263
+00:19:11,980 --> 00:19:17,460
+مالهاش أي دور في الموضوع هذا وهنا واضح الصورة كله
+
+264
+00:19:17,460 --> 00:19:21,980
+بتتبعهم الـ بارا سيمباتاتيك إيش الدور بتاعها و بارا
+
+265
+00:19:21,980 --> 00:19:25,380
+سيمباتاتيك نفس الشيء إيش الدور بتاعها هيك وصلنا
+
+266
+00:19:25,380 --> 00:19:30,800
+لنهاية المحاضرة يعطيكم ألف عافية ودومتم بخير
diff --git a/PL9fwy3NUQKwYBFfMQ7WkgiguUA_8aXeXd/vPEj4uiLQb4_postprocess.srt b/PL9fwy3NUQKwYBFfMQ7WkgiguUA_8aXeXd/vPEj4uiLQb4_postprocess.srt
new file mode 100644
index 0000000000000000000000000000000000000000..d4df1ae90b9a1eba2c32afe067231bdad6147097
--- /dev/null
+++ b/PL9fwy3NUQKwYBFfMQ7WkgiguUA_8aXeXd/vPEj4uiLQb4_postprocess.srt
@@ -0,0 +1,2120 @@
+1
+00:00:05,040 --> 00:00:08,620
+بسم الله الرحمن الرحيم السلام عليكم ورحمة الله
+
+2
+00:00:08,620 --> 00:00:15,120
+وبركاته نكمل محاضرة الأسبوع الحادي عشر على ال
+
+3
+00:00:15,120 --> 00:00:18,420
+somatic sensation أنا شرحتلكم اللي هو a
+
+4
+00:00:18,420 --> 00:00:23,220
+classification of somatic sensationشرحنا ان
+
+5
+00:00:23,220 --> 00:00:25,760
+somatic sensation مجسمهم according to site of
+
+6
+00:00:25,760 --> 00:00:29,720
+sensation ل superficial deep and combined
+
+7
+00:00:29,720 --> 00:00:33,640
+sensation وشرحت لكم classification of somatic
+
+8
+00:00:33,640 --> 00:00:39,440
+sensation according to the type of sensation it is
+
+9
+00:00:39,440 --> 00:00:43,640
+a more common classification divided into three
+
+10
+00:00:43,640 --> 00:00:48,660
+groups Meccano receptive sensationthermoreceptive
+
+11
+00:00:48,660 --> 00:00:52,800
+sensation and pain sensation واتكلمنا عنهم
+
+12
+00:00:52,800 --> 00:00:58,260
+بالتفصيل في الجزء الأول من المحاضرة الآن أنتجل إلى
+
+13
+00:00:58,260 --> 00:01:03,180
+موضوع pain sensation مهم جدا موضوع pain sensation
+
+14
+00:01:03,180 --> 00:01:10,460
+أن ال pain receptors بنقسمه إلى thermopain
+
+15
+00:01:10,460 --> 00:01:17,920
+receptorsكيمو pain receptors، ميكانو pain أو
+
+16
+00:01:17,920 --> 00:01:22,020
+ميكانو pain receptors أو بنسميهم ميكانو sensitive
+
+17
+00:01:22,020 --> 00:01:26,760
+pain receptor، التانين صح كيف ترمو pain receptor؟
+
+18
+00:01:26,760 --> 00:01:32,680
+أنا شربت شاي سخنشعرت أنه سخن و ألمني ألم يبقى هذا
+
+19
+00:01:32,680 --> 00:01:38,880
+يعتبر thermopain receptor شربت مياه باردة أو برد
+
+20
+00:01:38,880 --> 00:01:43,880
+أو الفكوية و ألمني هو بارد لكن بارد مع ألم يبقى
+
+21
+00:01:43,880 --> 00:01:49,420
+هذا يعتبر thermopain receptors chemoreceptors أو
+
+22
+00:01:49,420 --> 00:01:52,200
+chemopain receptors أكلت لمون و كان عندي جرح في
+
+23
+00:01:52,200 --> 00:01:58,270
+الشفةحمض مع قلم يبقى هذا يعتبر chemopain receptors
+
+24
+00:01:58,270 --> 00:02:01,610
+او جبت مواد كيموية حاططهم عيدي او حرجوا ايدي او
+
+25
+00:02:01,610 --> 00:02:05,750
+قلم يبقى هذي يعتبروا chemopain receptors و
+
+26
+00:02:05,750 --> 00:02:09,650
+mecanosensitive pain receptors ماشي واحد ماعم
+
+27
+00:02:09,650 --> 00:02:12,430
+يجلبه خبط رجله في الطاولة يبقى انتقلم هذا
+
+28
+00:02:12,430 --> 00:02:16,890
+mecanopain receptors طالبة اتناقضت مع أخوها ضربها
+
+29
+00:02:16,890 --> 00:02:23,540
+خمساوي هيك يعتبر هذه mecanopain receptorsAccording
+
+30
+00:02:23,540 --> 00:02:33,560
+to its site القلم حسب المكان بنقسم إلى يعني حسب
+
+31
+00:02:33,560 --> 00:02:41,760
+مكان ال stimulus Cotaneous سطحي على الجلد Deep
+
+32
+00:02:41,760 --> 00:02:48,820
+pain receptors يعني من joint من muscle من boneو
+
+33
+00:02:48,820 --> 00:02:52,540
+visceral pain يعني ألم من ال internal organs واحد
+
+34
+00:02:52,540 --> 00:02:55,520
+عنده appendicitis التهيأب في ال appendix و واحد
+
+35
+00:02:55,520 --> 00:03:00,080
+عنده حصوة في ال killer رينالكوليك و واحد عنده حصوة
+
+36
+00:03:00,080 --> 00:03:04,600
+في ال gallbladder كل هذا يعتبر visceral pain
+
+37
+00:03:04,600 --> 00:03:10,560
+receptors it is more frequently divided into و
+
+38
+00:03:10,560 --> 00:03:16,270
+الألم بنجسم إلى جسمينfast pain and slow pain مثال
+
+39
+00:03:16,270 --> 00:03:20,450
+على ال fast pain أنا بدي أخد injection حقنة أخدت
+
+40
+00:03:20,450 --> 00:03:25,470
+حقنة glial injection ألم شديد نخزع سريع هذا fast
+
+41
+00:03:25,470 --> 00:03:29,930
+pain روحت أدب طول أسنان أعطاني قبرة بنج ألم شعرت
+
+42
+00:03:29,930 --> 00:03:33,630
+فيه بسرعة this is fast pain ماشي على السجادة في
+
+43
+00:03:33,630 --> 00:03:38,230
+دبوس ضرب رجلي من تحت fast pain ألم سريع لكن slow
+
+44
+00:03:38,230 --> 00:03:42,970
+painواحد عنده التهيأب، deep venous thrombosis، ألم
+
+45
+00:03:42,970 --> 00:03:45,550
+في التجاربات في الأوردة، واحد عنده ألم في ال
+
+46
+00:03:45,550 --> 00:03:48,970
+muscles، وواحد عنده مشكلة في ال joints، خدنا يعتبر
+
+47
+00:03:48,970 --> 00:03:54,230
+ايش؟ slow pain، slow pain ال pathway ل ال pain
+
+48
+00:03:54,230 --> 00:04:00,490
+sensation it is lateral spinothalamic tract، راح
+
+49
+00:04:00,490 --> 00:04:04,610
+ناخده ان شاء الله اليوم central
+
+50
+00:04:04,610 --> 00:04:11,800
+perception of pain sensationالـ pen بيصلى الدماغ
+
+51
+00:04:11,800 --> 00:04:16,280
+عن طريق tract اسمه lateral بيمر في ال lateral
+
+52
+00:04:16,280 --> 00:04:20,720
+column و ال spinal cord، spino بيبدأ من ال spinal
+
+53
+00:04:20,720 --> 00:04:24,840
+cord، theramic tract و بينتهي في ال thalamus يبقى
+
+54
+00:04:24,840 --> 00:04:27,360
+ال tract موجود في ال lateral column اسمه lateral
+
+55
+00:04:28,130 --> 00:04:31,690
+ببدأ من Spinal cord Spinal و بنتهي في الثلامة
+
+56
+00:04:31,690 --> 00:04:35,250
+Thalamic يبقى من اللاترال Spinal Thalamic tract
+
+57
+00:04:35,250 --> 00:04:42,010
+هذا ال tract إله جزئين جزء اسمه Paleo Spinal
+
+58
+00:04:42,010 --> 00:04:49,570
+Thalamic tract و جزء ثاني اسمه NuSpinothalamic
+
+59
+00:04:49,570 --> 00:04:54,250
+tract هذول tract حسب نوع ال pain إذا كان ال pain
+
+60
+00:04:54,250 --> 00:05:00,150
+slow pain راح يمر في Paleo spinothalamic tract إذا
+
+61
+00:05:00,150 --> 00:05:05,790
+fast pain راح يمر في ال new spinothalamic tract
+
+62
+00:05:05,790 --> 00:05:12,370
+يبقى ال new spinothalamic tract مسئول عن ايه عن
+
+63
+00:05:12,370 --> 00:05:17,080
+fast painطب paleospinal nephrolipathy tract مسئول
+
+64
+00:05:17,080 --> 00:05:24,340
+عن ايه؟ عن slow pain تمام اخدنا في ال experiment
+
+65
+00:05:24,340 --> 00:05:28,640
+اعملنا experiment و احنا عارفين ال pain sensation
+
+66
+00:05:28,640 --> 00:05:32,720
+او ال sensation بتنتهي في ال boss central gyrus
+
+67
+00:05:32,720 --> 00:05:40,640
+area 123 اجالك ال pain sensationبصل الى area 123
+
+68
+00:05:40,640 --> 00:05:46,400
+يبقى post central gyrus تعتبر central area او
+
+69
+00:05:46,400 --> 00:05:52,220
+central cortical area for pain sensation اي pain
+
+70
+00:05:52,220 --> 00:05:57,240
+sensation؟ for fast pain sensation for fast pain
+
+71
+00:05:57,240 --> 00:06:02,540
+sensation طبعا slow pain وين بصل؟ ال slow pain
+
+72
+00:06:02,540 --> 00:06:08,470
+مابصلش ل area 123 بينتهي في الthalamusو الـ
+
+73
+00:06:08,470 --> 00:06:10,670
+reticular formation في البرنامج السنبلي فلو في
+
+74
+00:06:10,670 --> 00:06:15,810
+experiment أخدنا و عملنا rejection جسينا ال post
+
+75
+00:06:15,810 --> 00:06:19,050
+central gyrus و راميناها على الجط عاميناها post
+
+76
+00:06:19,050 --> 00:06:24,030
+central gyrus جسناها المريض بيبقى ليه شعور في ال
+
+77
+00:06:24,030 --> 00:06:29,210
+fast painبقل يشعرش في ال fast pain لكن ال slow
+
+78
+00:06:29,210 --> 00:06:33,850
+pain بشعر فيه، ليه؟ لأن ال slow pain بنتهي في ال
+
+79
+00:06:33,850 --> 00:06:38,170
+thalamus و في ال reticular formation و ما بيصل إلى
+
+80
+00:06:38,170 --> 00:06:43,870
+ال area 123 اللي موجودة في ال postcentral gyrus of
+
+81
+00:06:43,870 --> 00:06:49,110
+parietal lobe نتكلم على visceral pain الألم اللي
+
+82
+00:06:49,110 --> 00:06:50,590
+جاي من الأعضاء الداخلية
+
+83
+00:06:53,280 --> 00:06:57,320
+pain is almost the only sensation produced from
+
+84
+00:06:57,320 --> 00:07:02,600
+viscera ايش ال characteristics بتاعة visceral pain
+
+85
+00:07:02,600 --> 00:07:07,260
+ممكن يكون slow pain dull pain slow بنسميه dull
+
+86
+00:07:07,260 --> 00:07:11,580
+pain او spasmodic pain يعني ايش spasmodic يعني
+
+87
+00:07:11,580 --> 00:07:16,640
+colic pain مغز كلوي واحد عنده حصف مررة عملتله مغز
+
+88
+00:07:16,640 --> 00:07:22,270
+تمام يبقى هذا يعتبر colic pain اوfrequently
+
+89
+00:07:22,270 --> 00:07:27,130
+referred to a specific area in the skin والألم هذا
+
+90
+00:07:27,130 --> 00:07:32,110
+بيسمع في مكان على الجلد يعني كيف؟ يعني واحد عنده
+
+91
+00:07:32,110 --> 00:07:37,730
+حصوة في الكلاب، الألم ينزل
+
+92
+00:07:37,730 --> 00:07:42,730
+و referred لـinguinal area الميل بنزل لـscrotum،
+
+93
+00:07:42,730 --> 00:07:48,590
+لـtesticular area طيب مريض ثاني عنده gall bladder
+
+94
+00:07:48,590 --> 00:07:53,890
+stone عنده حصوة في المرارةالقلم ممكن يسمع و يشعر
+
+95
+00:07:53,890 --> 00:07:56,990
+.. و القلم radiating وين؟ لـ ال tip of shoulder
+
+96
+00:07:56,990 --> 00:08:00,390
+يطلع القلم فوق لل shoulder عن طريق frank's nerve
+
+97
+00:08:00,390 --> 00:08:05,180
+او the pragmatic nerveواضح؟ مريض عنده myocardial
+
+98
+00:08:05,180 --> 00:08:10,700
+infarction MI الألم ممكن radiating ل ال media ال
+
+99
+00:08:10,700 --> 00:08:13,480
+aspect of lower limb عشان هيك لما بيجيون مرضى و
+
+100
+00:08:13,480 --> 00:08:16,320
+خاصة كبار في السن عنده ألم في ال left shoulder
+
+101
+00:08:16,320 --> 00:08:19,020
+بنخاف يكون عنده جلطة في القلب بنعمل تختيط للقلب
+
+102
+00:08:19,020 --> 00:08:23,680
+ضروري لهم ليه؟ عشان الموضوع اللي هو ال myocardial
+
+103
+00:08:23,680 --> 00:08:29,420
+infarction هذا بنسميه referred painو usually
+
+104
+00:08:29,420 --> 00:08:34,200
+visceral pain associated with nausea,
+
+105
+00:08:34,440 --> 00:08:38,360
+parasympathetic effect يعني بيحدث معاهم غفيان،
+
+106
+00:08:38,360 --> 00:08:43,620
+تقيق و هكذا في بعض الأحيان associated with somatic
+
+107
+00:08:43,620 --> 00:08:47,060
+effect يعني واحد عنده appendicitis لو يجينا نعملنا
+
+108
+00:08:47,060 --> 00:08:51,200
+palpation لل abdomen بنلاقي tenderness في ال wall
+
+109
+00:08:51,200 --> 00:08:54,340
+of the abdomen بيصير tenderness في ال muscle
+
+110
+00:08:54,340 --> 00:08:59,300
+بنفسهم اللي هي skeletal muscleطب إيش هي irrevered
+
+111
+00:08:59,300 --> 00:09:02,520
+pain أو radiating pain اللي أنا شرحتها قبل شوية؟
+
+112
+00:09:02,520 --> 00:09:08,260
+this pain is felt away from the original site يعني
+
+113
+00:09:08,260 --> 00:09:13,400
+هو ألم المريض بيشعر فيه في مكان أخر مش في مكان
+
+114
+00:09:13,400 --> 00:09:17,880
+العضو المصاب it is commonly associated with
+
+115
+00:09:17,880 --> 00:09:22,030
+visceral pain أكتر شيء بنشوفه في visceral painPain
+
+116
+00:09:22,030 --> 00:09:24,910
+is usually referred to a somatic structure or
+
+117
+00:09:24,910 --> 00:09:29,070
+dermatome that developed from the same embryonic
+
+118
+00:09:29,070 --> 00:09:34,690
+segment which pain originated يعني القلم بيسمع في
+
+119
+00:09:34,690 --> 00:09:39,930
+المكان اللي على سبيل المثال ال heart بيسمع لل
+
+120
+00:09:39,930 --> 00:09:43,090
+upper limb في المكان اللي موجود المركز تبع ال
+
+121
+00:09:43,090 --> 00:09:46,870
+somatic area و ال visceral area نفس المركز يكون
+
+122
+00:09:46,870 --> 00:09:53,820
+قريب على بعض في نفس المكانهي عند مريض عنده
+
+123
+00:09:53,820 --> 00:09:57,220
+myocardial infarction جلطة في القلب الألم راح لل
+
+124
+00:09:57,220 --> 00:10:03,840
+segment أبارثوسك ومنه كمان شعر المريض أنه في ألم
+
+125
+00:10:03,840 --> 00:10:12,000
+في left shoulder and R example
+
+126
+00:10:13,220 --> 00:10:18,420
+example of referred pain امثلة على ال refered pain
+
+127
+00:10:18,420 --> 00:10:24,140
+inflammation of the gallbladder الاتهاب المرارة
+
+128
+00:10:24,140 --> 00:10:31,380
+الألم وين بيروح لل tip of right shoulder كتف
+
+129
+00:10:31,380 --> 00:10:35,560
+من أعلى cardiac pain الألم الناتج عن مشاكل في
+
+130
+00:10:35,560 --> 00:10:40,020
+القلببيروح لوين؟ لـ left arm لـ الـ immediate
+
+131
+00:10:40,020 --> 00:10:45,220
+aspect أو inner aspect of left arm وبعض الأحيان
+
+132
+00:10:45,220 --> 00:10:50,820
+لما بيكون inferior MI جلطة في القلب في الجلد
+
+133
+00:10:50,820 --> 00:10:54,780
+السفلي الألم بنزل للمعدة وكثير حالات بيجيوا على
+
+134
+00:10:54,780 --> 00:10:58,460
+استقبال مشاكل في المعدةوالدكتور ده كان صاحي يخطط
+
+135
+00:10:58,460 --> 00:11:00,720
+للقلب ممكن يكون عنده جلطة في القلب المريض جاي
+
+136
+00:11:00,720 --> 00:11:06,200
+الجلطة في الجزء الصوف باليمين من القلب والألم يضرب
+
+137
+00:11:06,200 --> 00:11:11,240
+وين؟ في الابيجاستروم في مكان المعدة ال kidney pain
+
+138
+00:11:11,240 --> 00:11:15,160
+أو ال gastric pain الألم ممكن ينزل للأمبولايكاس
+
+139
+00:11:15,160 --> 00:11:21,660
+حوالين سرعة ال kidney painالألم ممكن ينزل إلى أين؟
+
+140
+00:11:21,660 --> 00:11:23,940
+إلى الـ inguinal area زي ما اتكلمنا و إلى ال
+
+141
+00:11:23,940 --> 00:11:27,540
+testicular region ال pain اللي ناتج عن ال
+
+142
+00:11:27,540 --> 00:11:30,380
+appendicitis اللي في ال appendix ممكن الألم ينتج
+
+143
+00:11:30,380 --> 00:11:34,320
+إلى أين؟ إلى ال paraombolicus من ال right iliac
+
+144
+00:11:34,320 --> 00:11:41,300
+fossa إلى ال paraombolicus region الآن انا اتكلمت
+
+145
+00:11:41,300 --> 00:11:48,580
+على pain sensationاليوم انا بدي افهمكوا pathway of
+
+146
+00:11:48,580 --> 00:11:53,920
+superficial and pain sensation نشرح ال pathway
+
+147
+00:11:53,920 --> 00:11:56,900
+بتاع ال sensation على أساس انه في سنة رابعة ان
+
+148
+00:11:56,900 --> 00:11:59,980
+ضلينا طيبين لسنة رابعة لما أشرحلكوا ال spinal cord
+
+149
+00:11:59,980 --> 00:12:03,220
+lesions و الأمراض اللي بتصيب ال spinal cord انكون
+
+150
+00:12:03,220 --> 00:12:08,040
+عارفين أيش ال pathway و يكون أسهل لكم موضوع فهم
+
+151
+00:12:08,040 --> 00:12:16,120
+أيش الأمراض ال sensory pathway or axisالـ sensory
+
+152
+00:12:16,120 --> 00:12:20,880
+pathway include أي pathway لـ sensation، مهما كان
+
+153
+00:12:20,880 --> 00:12:24,140
+الـ sensation، لازم الإشارة تبدأ من الـ receptors،
+
+154
+00:12:24,140 --> 00:12:29,260
+مستقبل، بعد هيك، بتمر في sensory nerve، في عصب
+
+155
+00:12:29,260 --> 00:12:35,140
+حسي، بعد هيك، بمر في tract، في tract، وبيكون
+
+156
+00:12:35,140 --> 00:12:39,800
+ascending tract، afferent tractلحد ما يصل لوين ل
+
+157
+00:12:39,800 --> 00:12:44,760
+higher center و في النهاية بلزملي cortical central
+
+158
+00:12:44,760 --> 00:12:48,500
+area لما انا اتكلم عن ال motor العكس راح ابدأ من
+
+159
+00:12:48,500 --> 00:12:53,920
+cortical motor area بعدين tract بعدين motor nerve
+
+160
+00:12:53,920 --> 00:12:58,780
+بعدين muscle شوفته ال motor العكس descending بيكون
+
+161
+00:12:58,780 --> 00:13:05,300
+او afferent ال sensory عكسه ascending او afferent
+
+162
+00:13:06,960 --> 00:13:11,300
+All somatic sensations pass through three order
+
+163
+00:13:11,300 --> 00:13:15,320
+neurons كل somatic sensations بيمروا بكم نيرون؟
+
+164
+00:13:15,320 --> 00:13:17,480
+three order نيرون
+
+165
+00:13:24,310 --> 00:13:28,930
+بيكون وين موجود في ال peripheral nervous system في
+
+166
+00:13:28,930 --> 00:13:36,150
+ال ganglia اما ganglion of spinal nerve و اما
+
+167
+00:13:36,150 --> 00:13:40,430
+ganglion of cranial nerve ال second order نيرون
+
+168
+00:13:40,430 --> 00:13:43,810
+بيكون في ال central nervous system اما في ال
+
+169
+00:13:43,810 --> 00:13:48,250
+spinal cord و اما في ال brain stem قاعدة هي
+
+170
+00:13:48,250 --> 00:13:53,210
+القواعد نعملهم first
+
+171
+00:13:55,110 --> 00:14:04,530
+order راح يكون وين اما في spinal او cranial nerve
+
+172
+00:14:04,530 --> 00:14:14,290
+ganglion اول قاعدة طب second order راح
+
+173
+00:14:14,290 --> 00:14:20,450
+يكون اما في spinal cord موجود وين في spinal cord
+
+174
+00:14:23,100 --> 00:14:33,680
+in the spinal cord or brain stem
+
+175
+00:14:33,680 --> 00:14:44,620
+third order neuron تمام؟
+
+176
+00:14:44,620 --> 00:14:50,060
+رح يكون وين؟ في ال thalamusهو راح يكون في الـ
+
+177
+00:14:50,060 --> 00:14:53,860
+thalamus واضح؟ فالطريقة هذه انا بعمل اي pathway و
+
+178
+00:14:53,860 --> 00:14:56,900
+انطبقه على القاعدة هذه راح نلاحظ ان ال first order
+
+179
+00:14:56,900 --> 00:15:01,500
+موجود في spinal root جانجليون اما spinal و اما
+
+180
+00:15:01,500 --> 00:15:05,940
+cranial جانجليون second order اما بيكون في spinal
+
+181
+00:15:05,940 --> 00:15:08,840
+cord اما في ال brain stem و ال third order neuron
+
+182
+00:15:08,840 --> 00:15:13,000
+راح يكون في ال thalamus طريقة هذه انا بشرح اي
+
+183
+00:15:13,000 --> 00:15:21,320
+pathway اي pathway نبدأ الآنفي ال pathway of the
+
+184
+00:15:21,320 --> 00:15:25,460
+superficial sensation مابدأ أجيب الكلام أنا هذا
+
+185
+00:15:25,460 --> 00:15:34,160
+رسمة رسمها طلابي في الطب انتبع مع بعض ال first
+
+186
+00:15:34,160 --> 00:15:41,700
+order neuron ال first order neuron هي رح يكون
+
+187
+00:15:42,870 --> 00:15:46,450
+Posterior Root Ganglion Posterior Root Ganglion
+
+188
+00:15:46,450 --> 00:15:47,770
+Posterior Root Ganglion Posterior Root Ganglion
+
+189
+00:15:47,770 --> 00:15:48,990
+Posterior Root Ganglion Posterior Root Ganglion
+
+190
+00:15:48,990 --> 00:15:52,210
+Posterior Root Ganglion Posterior Root Ganglion
+
+191
+00:15:52,210 --> 00:15:57,150
+Posterior Root Ganglion Posterior Root Ganglion
+
+192
+00:15:57,150 --> 00:16:01,690
+Posterior Root Ganglion Posterior Root Ganglion
+
+193
+00:16:01,690 --> 00:16:03,530
+Posterior Root Ganglion Posterior Root Ganglion
+
+194
+00:16:03,530 --> 00:16:03,590
+Posterior Root Ganglion Posterior Root Ganglion
+
+195
+00:16:03,590 --> 00:16:04,930
+Posterior Root Ganglion Posterior Root Ganglion
+
+196
+00:16:04,930 --> 00:16:11,180
+Posterior Root Ganglion Posterior Root Ganglionفي
+
+197
+00:16:11,180 --> 00:16:16,620
+الـ Finger الإشارة اتحركت من الـ Receptors
+
+198
+00:16:16,620 --> 00:16:21,980
+للدندريت بتاع الـ First Order Neuron اللي هو
+
+199
+00:16:21,980 --> 00:16:26,630
+Spinal Sensory Nerve Fibersبعد ذلك راحت وعملت
+
+200
+00:16:26,630 --> 00:16:30,970
+relay في ال first order نيرون في first order نيرون
+
+201
+00:16:30,970 --> 00:16:35,130
+يجي مين هو ال first order نيرون هو posterior root
+
+202
+00:16:35,130 --> 00:16:38,630
+ganglion بعدها الإشارة بتروح بال axon of first
+
+203
+00:16:38,630 --> 00:16:44,970
+order نيرون بتمر في ال posterior root nerve أو في
+
+204
+00:16:44,970 --> 00:16:51,730
+ال posterior root بتروح ل ال posterior horn cells
+
+205
+00:16:51,730 --> 00:16:58,920
+في ال posterior horn cellsفى شىء اسمه Substantia
+
+206
+00:16:58,920 --> 00:17:02,900
+Gelatinosa Overlandii مكتوب عندكوا فى ال slide راح
+
+207
+00:17:02,900 --> 00:17:07,740
+نشوفها Substantia Gelatinosa Overlandii يبقى نتبع
+
+208
+00:17:07,740 --> 00:17:12,580
+ال second order نيرون وين موجود؟ Spinal cord or
+
+209
+00:17:12,580 --> 00:17:16,120
+brainstem يبقى صح هذا فى ال spinal cord يبقى ال
+
+210
+00:17:16,120 --> 00:17:20,640
+second order نيرون موجود وين؟ فى ال posterior horn
+
+211
+00:17:20,640 --> 00:17:27,200
+cells اسمهاsubstantia gelatinosa of Rolandia لحظة
+
+212
+00:17:27,200 --> 00:17:31,020
+هنا أخدنا segment واخدنا segment different segment
+
+213
+00:17:31,020 --> 00:17:35,780
+بعد الإشارة تعمل ال crossing directly تعمل ال
+
+214
+00:17:35,780 --> 00:17:39,840
+crossing directly في ال entry of spinal cord و
+
+215
+00:17:39,840 --> 00:17:42,580
+بتتجه إلى ال opposite side of spinal cord بتنتج
+
+216
+00:17:42,580 --> 00:17:46,480
+إلى وين؟ إلى ال opposite side of spinal cord بتطلع
+
+217
+00:17:46,480 --> 00:17:51,680
+في شيء اسمهLateral spinothalamic tract اللي انا
+
+218
+00:17:51,680 --> 00:17:55,400
+شرحته موجود
+
+219
+00:17:55,400 --> 00:17:59,760
+في lateral ايش column spinal cord ascending tract
+
+220
+00:17:59,760 --> 00:18:03,520
+بفضل spinal cord بعد ذلك الإشارة بتمر في ال brain
+
+221
+00:18:03,520 --> 00:18:09,730
+stemالبرنامج سلم وبتجابل فيه track ثاني اسمه
+
+222
+00:18:09,730 --> 00:18:13,570
+ventral spine metallic tract ماشرحتوش هذا لكروت
+
+223
+00:18:13,570 --> 00:18:18,750
+تتش لللمس مع ال lateral spine metallic tract بيصير
+
+224
+00:18:18,750 --> 00:18:25,600
+اسمهم spinal lemniscus بصعد هذا ال trackهو المنسكس
+
+225
+00:18:25,600 --> 00:18:31,380
+ميضالة أبلانجاتا فونز ميديبرين وبنتهي وين في
+
+226
+00:18:31,380 --> 00:18:35,440
+الثلمس خلصنا ال second order نيرون بيبدأ مين ال
+
+227
+00:18:35,440 --> 00:18:38,040
+third order نيرون قولنا وين ال third order نيرون
+
+228
+00:18:38,040 --> 00:18:42,120
+في الثلمس يبقى نهاية عندي ال third order نيرون
+
+229
+00:18:42,120 --> 00:18:46,620
+بالأخضر وين موجود في الثلمس ال pathway بتاعته بتمر
+
+230
+00:18:46,620 --> 00:18:51,300
+في ال posterior limb of internal capsule لحد ما
+
+231
+00:18:51,300 --> 00:18:58,520
+نصلArea 123 Area 123 بالذكرين الإنسان كيه بيكون
+
+232
+00:18:58,520 --> 00:19:05,420
+قولنا هيك الإنسان بيكون رأسه تحت إيديه
+
+233
+00:19:05,420 --> 00:19:08,640
+في
+
+234
+00:19:08,640 --> 00:19:12,700
+الوسط وال lower limbs فوق يبقى ده إذا الإشارة جاية
+
+235
+00:19:12,700 --> 00:19:19,320
+من ال faceبيروح لل inferior postcentral gyrus إذا
+
+236
+00:19:19,320 --> 00:19:24,380
+الإشارة جاية من ال foot من القدم بيروح لل superior
+
+237
+00:19:24,380 --> 00:19:29,500
+postcentral gyrus يبقى الإنسان في الدماغ العكس و
+
+238
+00:19:29,500 --> 00:19:33,740
+احنا هنا رأسنا فوق رجلينا تحت إذا الإنسان محكم
+
+239
+00:19:33,740 --> 00:19:37,300
+بيكونش بيصير محروم رأسه تحت رجليه فوق و هيك
+
+240
+00:19:37,300 --> 00:19:43,340
+الإنسان موجود في الدماغ رأسه تحت و رجليه فوق يبقى
+
+241
+00:19:43,340 --> 00:19:43,920
+كمان مرة
+
+242
+00:19:47,270 --> 00:19:51,810
+نشرح ال pain temperature sensationالإشارة أجت من
+
+243
+00:19:51,810 --> 00:19:55,770
+الـ Receptors، راحت للـ Peripheral nerve، راحت للـ
+
+244
+00:19:55,770 --> 00:19:59,050
+First Order Neuron، مين هو ال First Order Neuron؟
+
+245
+00:19:59,050 --> 00:20:03,130
+Posterior Root Ganglion، بعد هيك بتمر في ال Axon
+
+246
+00:20:03,130 --> 00:20:06,530
+of the First Order Neuron، اسمه Posterior Root،
+
+247
+00:20:06,530 --> 00:20:10,250
+راح تصل لوين؟ للبوستيريو هور سيلز، في البوستيريو
+
+248
+00:20:10,250 --> 00:20:15,030
+هور سيلز في Second Order Neuron، اسمه Substantia
+
+249
+00:20:15,030 --> 00:20:18,490
+Gelatinosa Overlandii، بعدها الفيبرز،
+
+250
+00:20:20,730 --> 00:20:24,950
+فى ال entry of spinal cord كل segment بعمل
+
+251
+00:20:24,950 --> 00:20:28,770
+crossing فى المدخل بتاعه و ال crossing بتروح لل
+
+252
+00:20:28,770 --> 00:20:32,290
+opposite side و بيصعدوا فى tract اسمه lateral
+
+253
+00:20:32,290 --> 00:20:36,390
+spinothalamic tract for pain and temperature
+
+254
+00:20:36,390 --> 00:20:41,100
+sensationوبالتحت في براين السماعات track ثاني اسمه
+
+255
+00:20:41,100 --> 00:20:45,160
+Ventral Spinal Thalamic Track مسئول عن الكروت تتش
+
+256
+00:20:45,160 --> 00:20:49,680
+وبصبح اسمهم اللي هو Spinal Meniscus بيمروا في
+
+257
+00:20:49,680 --> 00:20:54,200
+الميدالة أبلانجاتا في ال Pons Midbrain and ال End
+
+258
+00:20:54,200 --> 00:20:58,600
+تبعهم للثلامس من الثلامس بيبدأ مين؟ Third Order
+
+259
+00:20:58,600 --> 00:21:02,220
+Neuron النيرون الثالث بيمروا ال fibers بعته عن
+
+260
+00:21:02,220 --> 00:21:06,700
+طريق ثلامهCortical tract flammocortical tract
+
+261
+00:21:06,700 --> 00:21:10,180
+بمروا في البستيرو الليمو و بنتيرنا الكابسول لحد ما
+
+262
+00:21:10,180 --> 00:21:14,840
+نصل لل boss central gyrus إذا الإشارة جاية من ال
+
+263
+00:21:14,840 --> 00:21:18,620
+lower limb بتروح لل upper part إذا الإشارة جاية من
+
+264
+00:21:18,620 --> 00:21:21,940
+ال trunk أو ال upper limbs بتروح لل middle part
+
+265
+00:21:21,940 --> 00:21:25,000
+إذا الإشارة جاية من ال head أو ال face بتروح لل
+
+266
+00:21:25,000 --> 00:21:29,920
+lower part عكسينرجح نشوف ال slide هين شرحناها على
+
+267
+00:21:29,920 --> 00:21:34,480
+الصورة ننتقل لان لال slide بتاعنا اللي انا شرحته
+
+268
+00:21:34,480 --> 00:21:38,700
+pathway
+
+269
+00:21:38,700 --> 00:21:41,780
+of superficial sensation from the upper and lower
+
+270
+00:21:41,780 --> 00:21:42,180
+limbs
+
+271
+00:21:45,410 --> 00:21:49,230
+للـ Pain Temperature Sensation First Order Neuron
+
+272
+00:21:49,230 --> 00:21:53,710
+زي ما اتفجنا بوستيريو رود جانجليون كله شراحته هذا
+
+273
+00:21:53,710 --> 00:21:58,310
+الـ Dendrite بتجيب الإشارة من الـ Receptors عن
+
+274
+00:21:58,310 --> 00:22:02,910
+طريق Sensory Nerve Fibers وبتسلمها لل First Order
+
+275
+00:22:02,910 --> 00:22:08,180
+Neuron اللي هو بوستيريو رود جانجليون الـ Axonاللي
+
+276
+00:22:08,180 --> 00:22:12,040
+هو media ال branch هتكون، بدخل لل spinal cord،
+
+277
+00:22:12,040 --> 00:22:16,920
+بيمر ل ال posterior horn cells، و بيسلم الإشارة ل
+
+278
+00:22:16,920 --> 00:22:20,940
+ال second order neuron، اللي هي اسمها substantia
+
+279
+00:22:20,940 --> 00:22:25,000
+gelatinosa overlandii، يعني فكان بطلع بال slide
+
+280
+00:22:25,000 --> 00:22:28,020
+هذا، مين first order neuron؟ posterior root
+
+281
+00:22:28,020 --> 00:22:32,800
+ganglionمين second order؟ سبستانشيا، جيلاتنوزا أو
+
+282
+00:22:32,800 --> 00:22:36,240
+رولاندي، كل segment له سبستانشيا، جيلاتنوزا أو
+
+283
+00:22:36,240 --> 00:22:41,000
+رولاندي، وكل segment له بوستيريون، رهود، جانجليون
+
+284
+00:22:43,190 --> 00:22:46,230
+ننتقل للـ second order neuron مين هو second order
+
+285
+00:22:46,230 --> 00:22:51,410
+neuron؟ Substantia gelatinosa of رولندي it's axon
+
+286
+00:22:51,410 --> 00:22:54,630
+it cross to the opposite side وقولنا الفايفر
+
+287
+00:22:54,630 --> 00:22:58,030
+سبعتهم من حد ما يفوتوا في ال spinal cord بيعمل
+
+288
+00:22:58,030 --> 00:23:01,350
+dedication أو crossing على ال opposite side و
+
+289
+00:23:01,350 --> 00:23:06,830
+بيمروا لل lateral spinothalamic tract زي أنا جاي
+
+290
+00:23:06,830 --> 00:23:11,440
+من شريع فرعيوبدي امر على الشارع الرئيسي و اه امر
+
+291
+00:23:11,440 --> 00:23:15,180
+بمركب السيارات نفس الشيء الشارع الفرقي المركب
+
+292
+00:23:15,180 --> 00:23:18,080
+السيارات الشارع الرئيسي صلاح الدين هذا ال tract
+
+293
+00:23:18,080 --> 00:23:22,320
+هذا مين هو؟ ال tract وكل خط فرقي بيجي و بيصب مع
+
+294
+00:23:22,320 --> 00:23:26,180
+مين؟ مع الخط الرئيسي يبقى ال tract صلاح الدين شارع
+
+295
+00:23:26,180 --> 00:23:29,940
+صلاح الدين okay يبقى lateral spinal thalamic tract
+
+296
+00:23:29,940 --> 00:23:35,320
+بيمر للميدالة و بلانجاتهاPons Medibrain وبعد ذلك
+
+297
+00:23:35,320 --> 00:23:39,480
+ينتهي في الـThalamus ثالث نيرون يسمى الـThalamus
+
+298
+00:23:39,480 --> 00:23:43,140
+الفيبرز تصعد عن طريق الـPosterior Lymph of
+
+299
+00:23:43,140 --> 00:23:46,860
+Internal Capsule تمر في Tract اسمه Thalamocortical
+
+300
+00:23:46,860 --> 00:23:52,660
+Tract وبتنتهي في الـArea 123 in both central gyrus
+
+301
+00:23:52,660 --> 00:23:57,270
+of Parietal Lobe هكذا انا شرحت لكم ال path wayof
+
+302
+00:23:57,270 --> 00:24:01,210
+pain temperature sensation اليوم ممكن اي طالب و اي
+
+303
+00:24:01,210 --> 00:24:05,070
+طالبة يروح يشخص على الوالدة يغزها دبوس في ايدها و
+
+304
+00:24:05,070 --> 00:24:07,990
+يقولها اريت اشرحلك ال path way ايش صار معاك في
+
+305
+00:24:07,990 --> 00:24:11,230
+احساس الدبوس و كيف انت حسيتي في الدبوس و في الألم
+
+306
+00:24:11,230 --> 00:24:14,110
+او يجيبليها ايش سخن بس ما تكويش ايدها اللي انت
+
+307
+00:24:14,110 --> 00:24:17,910
+حارقها بالمرة و يحطلها ميا سخنة على ايدها و يقولها
+
+308
+00:24:17,910 --> 00:24:22,980
+اريت اشرحلك كيف انت حسيتي بالميا السخنة كلهاهي
+
+309
+00:24:22,980 --> 00:24:26,520
+عندنا لحظة هذا ال pathway انا شرحته في ال slide
+
+310
+00:24:26,520 --> 00:24:30,360
+بتاعي هى ال receptors بتروح لل first order neuron
+
+311
+00:24:30,360 --> 00:24:33,660
+اللى هو posterior root ganglion بيمروا في ال
+
+312
+00:24:33,660 --> 00:24:36,680
+posterior root تمام هان ال receptors هى ال sensory
+
+313
+00:24:36,680 --> 00:24:41,780
+nerve لل second order neuron هذا first هذا second
+
+314
+00:24:42,920 --> 00:24:45,060
+substance gelatinosa of Rolandii الـ fibers
+
+315
+00:24:45,060 --> 00:24:47,860
+بيعملوا crossing و بيطلعوا في ال lateral
+
+316
+00:24:47,860 --> 00:24:50,200
+spinothalamic tract نفس الشيء عضاء ال segment ثاني
+
+317
+00:24:50,200 --> 00:24:53,680
+lateral spinothalamic tract بيمروا في ال brain
+
+318
+00:24:53,680 --> 00:24:57,840
+stem لحد ما نصل لوين ل third order neurothalamus
+
+319
+00:24:57,840 --> 00:25:01,280
+بعدين بيروحوا ل post central gyrus هيك ال pathway
+
+320
+00:25:01,280 --> 00:25:05,080
+of pain temperature sensation أنا ماشي في الطريق
+
+321
+00:25:05,080 --> 00:25:10,820
+قلت ده بقص الرجلين الإشارة
+
+322
+00:25:10,820 --> 00:25:15,730
+طلتمع ال spinal nerve اللي هو ديندرية تبع first
+
+323
+00:25:15,730 --> 00:25:20,830
+order neuron هي ال first order neuron الإشارة راحت
+
+324
+00:25:20,830 --> 00:25:26,070
+ل spinal cord ل second order neuron ال fibers
+
+325
+00:25:26,070 --> 00:25:28,940
+عملوا crossingو راحوا لوين؟ لـ lateral
+
+326
+00:25:28,940 --> 00:25:32,460
+spinothalamic tract عملوا relay وين؟ في الـ third
+
+327
+00:25:32,460 --> 00:25:37,400
+order neurothalamus بعد هيك ل area A123 اللي هي
+
+328
+00:25:37,400 --> 00:25:44,060
+cortical area لحظة لو جينا مريض انقص عنده عصب بطر
+
+329
+00:25:44,060 --> 00:25:48,400
+أو إصابة، إصابة في رجله، انقص العصب، بيبطل يشعر في
+
+330
+00:25:48,400 --> 00:25:54,650
+الدرماتومة تبعته، تماممريض حدث عنده حزام ناري و
+
+331
+00:25:54,650 --> 00:26:01,410
+صاب الجانجليوم herpes zoster virus بشعر بقلم في
+
+332
+00:26:01,410 --> 00:26:03,410
+نفس ال root في نفس النيرب على نفس الجهة و بيكون
+
+333
+00:26:03,410 --> 00:26:07,790
+عنده vesicles زي طفح جلد فيه طيب مريض صار مشكلة
+
+334
+00:26:07,790 --> 00:26:10,670
+أنه في البستيرو horn cells نفس الشيء في نفس ال
+
+335
+00:26:10,670 --> 00:26:14,210
+dermatome ايه بيبطل الحس طب لو واحد أجي عنده
+
+336
+00:26:14,210 --> 00:26:19,190
+الصاصة و قطعت ال spinal cord على ال right sideإيش
+
+337
+00:26:19,190 --> 00:26:22,750
+بيصير في ال pain temperature sensation بيصير loss
+
+338
+00:26:22,750 --> 00:26:29,090
+of pain temperature sensation below the level تحت
+
+339
+00:26:29,090 --> 00:26:33,050
+مستوى الإصابة بس على أي جهة؟ على ال opposite side
+
+340
+00:26:33,050 --> 00:26:36,310
+وين بتكون؟ على إيه؟ opposite side طب ليش على ال
+
+341
+00:26:36,310 --> 00:26:40,310
+opposite side يا دكتور؟ لإنه ال tract right spinal
+
+342
+00:26:40,310 --> 00:26:43,230
+thalamic tract ال fibers ده بعتجبها من ال left في
+
+343
+00:26:43,230 --> 00:26:46,170
+الحلقة ده انضرب ال right و راح يبطل يشعر في ال
+
+344
+00:26:46,170 --> 00:26:51,010
+left مريض صار معاه جلطة دماغيةفي ال area 123 بيصير
+
+345
+00:26:51,010 --> 00:26:53,850
+فقدان الإحساس على ال opposite side مريض حدث مع
+
+346
+00:26:53,850 --> 00:26:56,930
+جلطه دماغية في الطلمس بيصير عنده فقدان الإحساس
+
+347
+00:26:56,930 --> 00:27:00,770
+عوين على ال opposite side واضح؟ يبقى بعد ما يعملوا
+
+348
+00:27:00,770 --> 00:27:03,770
+fibers crossing إذا صار إصابة على ال opposite side
+
+349
+00:27:03,770 --> 00:27:05,990
+قبل ما يعملوا crossing على نفس ال side
+
+350
+00:27:12,150 --> 00:27:18,530
+يعني انا شرحتلكوا بطريقة مملة و بزراحة و بزيادة ما
+
+351
+00:27:18,530 --> 00:27:22,610
+تفهموها اشهر هذه الموضوع مهم جدا لو كنت اتأخر فيه
+
+352
+00:27:22,610 --> 00:27:28,330
+في المحاضرة مش مشكلة بدى تفهموه صح هاي عين دى
+
+353
+00:27:28,330 --> 00:27:31,550
+البني هذا اللون او الأصفر هذا deep sensation مارح
+
+354
+00:27:31,550 --> 00:27:35,170
+اشرحه الآن نشوف ال pain temperature هاي receptors
+
+355
+00:27:35,170 --> 00:27:39,050
+sensorineurبوستيريو روت جانجليون first order
+
+356
+00:27:39,050 --> 00:27:42,710
+neuron الإشارة على ال بوستيريو روت على second
+
+357
+00:27:42,710 --> 00:27:45,070
+order neuron substantia gelatinosa of رولاندي
+
+358
+00:27:45,070 --> 00:27:48,550
+عملوا crossing على ال intro of spinal cord طلوا
+
+359
+00:27:48,550 --> 00:27:51,590
+على اسبينيو تلاميكي 3 اكتاترا لمر في ال brain السم
+
+360
+00:27:51,590 --> 00:27:57,910
+وبعد هيك راح لوين ل third order neuron تلامس ومر
+
+361
+00:27:57,910 --> 00:28:01,190
+في البوستيريو لمبوف انترنال كابسول لحد ما نصل ال
+
+362
+00:28:01,190 --> 00:28:08,430
+area onetwo three هيكا بيكون انا شرحتلكوا pain
+
+363
+00:28:08,430 --> 00:28:19,610
+temperature sensation هيكا
+
+364
+00:28:19,610 --> 00:28:23,890
+الإنسان بيكون في الدماغ الرأس تحت ال upper limbs
+
+365
+00:28:23,890 --> 00:28:27,170
+في النص و ال lower limbs في الأعلى و ال lower
+
+366
+00:28:27,170 --> 00:28:32,740
+limbs في الأعلىال pathway of touch sensation، ال
+
+367
+00:28:32,740 --> 00:28:37,760
+touch sensation اللامس من قسم إلى قسمينلو جيبنا
+
+368
+00:28:37,760 --> 00:28:41,840
+الجلم سن الجلم حطته على رأسها ده او الإبرة شرطها
+
+369
+00:28:41,840 --> 00:28:46,180
+هذي fine touch يعني fine touch good localized
+
+370
+00:28:46,180 --> 00:28:51,100
+touch sensation ال fine touch مارح اتكلم عنها لأن
+
+371
+00:28:51,100 --> 00:28:54,440
+ال pathway بتاعها اسمه gracile and connective
+
+372
+00:28:54,440 --> 00:28:58,540
+tract ال fine touch نفس ال pathway بتاع ال deep
+
+373
+00:28:58,540 --> 00:29:02,200
+sensation يعني ال fine touch رح اشرحوا مع وين مع
+
+374
+00:29:02,200 --> 00:29:05,980
+ال deep sensation مع مين مع ال deep sensation okay
+
+375
+00:29:06,630 --> 00:29:10,810
+طب الكروت تاتش، إيش كروت تاتش؟ هاي جيبنا ورقة،
+
+376
+00:29:10,810 --> 00:29:14,270
+حطيناها على وجهها، على الخد، لمسناها، في حاسة لمس
+
+377
+00:29:14,270 --> 00:29:18,410
+فيه، هذا اسمه كروت تاتش، الستات حطوا الطرحة على
+
+378
+00:29:18,410 --> 00:29:22,970
+راسهم، هذا اسمها كروت تاتش، يعني poor localized
+
+379
+00:29:22,970 --> 00:29:29,510
+touch sensation الكروت تاتش، ال pathway بتاعه،
+
+380
+00:29:29,510 --> 00:29:34,190
+بشبه ال pain temperature sensationبشبه مين؟ pain,
+
+381
+00:29:34,370 --> 00:29:37,590
+temperature, sensation وين الاختلاف؟ الاختلاف في
+
+382
+00:29:37,590 --> 00:29:44,990
+حاجتين اول شيء ال first order نيرون نفس الشيء
+
+383
+00:29:44,990 --> 00:29:50,150
+posterior root ganglion و ال third order نفس الشيء
+
+384
+00:29:50,150 --> 00:29:51,530
+thalamus
+
+385
+00:30:00,110 --> 00:30:04,310
+الاختلاف في اين؟ في ال second order neuron ال
+
+386
+00:30:04,310 --> 00:30:06,710
+second order neuron بدل ما هي اسمها substantia
+
+387
+00:30:06,710 --> 00:30:13,090
+gelatinosa overlandii اسمها main sensory nucleus
+
+388
+00:30:13,090 --> 00:30:20,450
+ايش اسمها؟ main sensory nucleus اسمها main sensory
+
+389
+00:30:23,730 --> 00:30:28,310
+الحاجة الثالثة ال pathway تبعها بدل ما يعمل ال
+
+390
+00:30:28,310 --> 00:30:32,150
+crossing ويروح على ال lateral column بيروح على ال
+
+391
+00:30:32,150 --> 00:30:36,670
+ventral column و بيصعد في ال track اسمه ventral
+
+392
+00:30:36,670 --> 00:30:41,520
+columnSpinothalamic tract يبقى نهايه اختلافين انه
+
+393
+00:30:41,520 --> 00:30:46,320
+ال tract تبعهم اسمه ventral spinothalamic tract و
+
+394
+00:30:46,320 --> 00:30:49,900
+ال second order neuron اسمها main sensory nucleus
+
+395
+00:30:49,900 --> 00:30:53,620
+و الباقي نفس ال pain temperature sensation يبقى
+
+396
+00:30:53,620 --> 00:30:59,300
+نهايه عند الكروتتش has the same pathway as pain
+
+397
+00:30:59,300 --> 00:31:03,720
+and temperature sensation but the second order
+
+398
+00:31:03,720 --> 00:31:08,700
+neuron is main sensory nucleusin the posterior
+
+399
+00:31:08,700 --> 00:31:13,860
+horn cells and ascend in the opposite ventral
+
+400
+00:31:13,860 --> 00:31:20,160
+column of spinal cord as ventral spinothalamic
+
+401
+00:31:20,160 --> 00:31:23,920
+tract وغير هيك كله نفس الشيء كله نفس ال pain
+
+402
+00:31:23,920 --> 00:31:27,360
+temperature sensation يقول هيك أنا خلصتلكم موضوع
+
+403
+00:31:27,360 --> 00:31:32,420
+ال touch sensation ال touch sensation أنتج لأخر
+
+404
+00:31:32,420 --> 00:31:39,360
+موضوع في المحاضرة اللي هو pathwayof deep sensation
+
+405
+00:31:39,360 --> 00:31:43,680
+pathway of deep sensation نشرحه هنا على ال slide
+
+406
+00:31:43,680 --> 00:31:47,900
+اللي أنا لا اسمه ولا كلات بالطب أجتني إشارة من ال
+
+407
+00:31:47,900 --> 00:31:52,620
+receptors من ال joint من أيه؟ من ال joint يبقى أن
+
+408
+00:31:52,620 --> 00:31:58,180
+هذه من upper limb على سبيل المثال وهذه من وين؟ من
+
+409
+00:31:58,180 --> 00:32:05,990
+lower limb من وين؟ من ال lower limb ال ..لكن في ال
+
+410
+00:32:05,990 --> 00:32:09,450
+slide أنا في ال slide المرسوم كله يعتبر upper limb
+
+411
+00:32:09,450 --> 00:32:12,190
+ماعلينا خلّيني اعتبره ايش من ال upper limb الثانية
+
+412
+00:32:12,190 --> 00:32:18,870
+من upper limb الإشارة تيجي من ال receptors تبع ال
+
+413
+00:32:18,870 --> 00:32:22,650
+joint او ال ligament او muscle نفس الشيء نفس ال
+
+414
+00:32:22,650 --> 00:32:26,470
+temperature أوروبيا بتروح على ال sensory nerve نفس
+
+415
+00:32:26,470 --> 00:32:32,210
+الشيء ال first order neuronوالـ third order neuron
+
+416
+00:32:32,210 --> 00:32:35,170
+نفس الشيء نفس الـ pain و ال temperature ال first و
+
+417
+00:32:35,170 --> 00:32:39,190
+ال third نفس الشيء اختلاف ال second ال fibers
+
+418
+00:32:39,190 --> 00:32:45,720
+تبعتهم بتروح في ال posterior ايش؟ rootفي ال
+
+419
+00:32:45,720 --> 00:32:50,720
+posterior root بعدين، لحظة، بتفوتش لل posterior
+
+420
+00:32:50,720 --> 00:32:57,140
+horn cells، تصعد في شيء اسمه connect tract وشيء
+
+421
+00:32:57,140 --> 00:33:01,980
+اسمه gracile tract، ملاحظة، gracile tract بيكون
+
+422
+00:33:01,980 --> 00:33:06,210
+more media، يبقى ان هذا gracileهذا ايه؟ Grassel و
+
+423
+00:33:06,210 --> 00:33:11,110
+هذا ايه؟ Conate ل Grassel بيكون more medial و
+
+424
+00:33:11,110 --> 00:33:14,710
+مسئول عن ال lower half of the body يبقى مين مسئول
+
+425
+00:33:14,710 --> 00:33:18,150
+عن حركة ال knee joint في ال lower limb؟ Grassel
+
+426
+00:33:18,150 --> 00:33:21,830
+attract مين مسئول عن ال elbow joint؟ Conate
+
+427
+00:33:21,830 --> 00:33:25,010
+attract Conate lateral مسئول عن ال upper half of
+
+428
+00:33:25,010 --> 00:33:28,350
+the body Grassel medial مسئول عن ال lower half of
+
+429
+00:33:28,350 --> 00:33:32,660
+the bodyلاحظوا في الـ spinal cord الـ deep
+
+430
+00:33:32,660 --> 00:33:34,920
+sensation ماعملش crossing، بيروحش على الـ opposite
+
+431
+00:33:34,920 --> 00:33:39,820
+side، بصعد على نفس الصعيد، في شيء اسمه connect and
+
+432
+00:33:39,820 --> 00:33:45,060
+gracellic tract، ماعملش decussion، و بمر على
+
+433
+00:33:45,060 --> 00:33:48,420
+المضلة أبل أن جاتها، gracellic tract بيسلم
+
+434
+00:33:48,420 --> 00:33:51,340
+gracellic nucleus، و connectic tract بيسلم
+
+435
+00:33:51,340 --> 00:33:54,760
+connectic nucleus، و لحد الآن ماعملش crossing،
+
+436
+00:33:54,760 --> 00:34:00,590
+يبقى مين ال second order نيرون؟ هايهمSecond Order
+
+437
+00:34:00,590 --> 00:34:06,510
+Neuron Igracel and Conate Nuclei in Medulla
+
+438
+00:34:06,510 --> 00:34:10,270
+Blangata هل هذا بنطبق؟ Second Order Neuron إما في
+
+439
+00:34:10,270 --> 00:34:13,270
+الـSpinal Cord أو في الـBrain Slim صح Medulla
+
+440
+00:34:13,270 --> 00:34:17,480
+Blangata في الـBrain Slimبعدها ال fibers بيعملوا
+
+441
+00:34:17,480 --> 00:34:21,760
+crossing و بيطلعوا في شيء اسمه media lemniscas و
+
+442
+00:34:21,760 --> 00:34:26,020
+بيسلموا منه ال third order neuron thalamus وبعدها
+
+443
+00:34:26,020 --> 00:34:29,420
+نفس الشيء بيطلعوا cortical pathway من بوستيرو لمبو
+
+444
+00:34:29,420 --> 00:34:34,090
+فنتيرون الكابسول ل area 123يبقى الاختلاف هنا في ال
+
+445
+00:34:34,090 --> 00:34:37,650
+second order neuron اول اختلاف ان ال second order
+
+446
+00:34:37,650 --> 00:34:40,170
+neuron موجود في ال brainstem في الميضا اللي قبل ان
+
+447
+00:34:40,170 --> 00:34:44,470
+جاتها واسمه gracile and conate nuclei، gracile
+
+448
+00:34:44,470 --> 00:34:49,790
+tract و gracile nucleus مسئولين عن ال lower half
+
+449
+00:34:49,790 --> 00:34:53,450
+of the body، conate tract و conate nucleus مسئولين
+
+450
+00:34:53,450 --> 00:34:58,070
+عن ال upper half of the body، لمين؟deep sensation
+
+451
+00:34:58,070 --> 00:35:03,430
+and fine touch يبقى لو أصار damage لأ grassy tract
+
+452
+00:35:03,430 --> 00:35:08,890
+او conient tract بيصير loss of deep sensation and
+
+453
+00:35:08,890 --> 00:35:14,550
+fine touch below the level of lesion إبسي لاترالة
+
+454
+00:35:14,550 --> 00:35:17,590
+على نفس الجهةليش على نفس الجهة؟ لأنه ماعملوش
+
+455
+00:35:17,590 --> 00:35:20,950
+crossing ماعملوش crossing لكن لو صار damage على ال
+
+456
+00:35:20,950 --> 00:35:24,850
+media lemniscus او على ال thalamus او area 123
+
+457
+00:35:24,850 --> 00:35:27,230
+بيصير lost بس على ال opposite side يبقى ان ده ال
+
+458
+00:35:27,230 --> 00:35:30,510
+damage قبل ال decassation على نفس ال side بعد ال
+
+459
+00:35:30,510 --> 00:35:32,410
+decassation على ال opposite side
+
+460
+00:35:41,450 --> 00:35:44,530
+هي عندى ال first order neuron هو بوستيرو
+
+461
+00:35:44,530 --> 00:35:49,800
+رودجانجليون نفس الموضوع ال pain temperatureالإشارة
+
+462
+00:35:49,800 --> 00:35:53,400
+بتيجي من الـ receptors بتروح مع اللي هي sensory
+
+463
+00:35:53,400 --> 00:35:57,040
+nerve بعد هي اللي بتسلم لل first order نيرون
+
+464
+00:35:57,040 --> 00:36:01,940
+بوستيرو دجانجليون ال tract او ال axon بتاعها بيمر
+
+465
+00:36:01,940 --> 00:36:05,140
+في ال spinal cord بدخلش على ال posterior horn
+
+466
+00:36:05,140 --> 00:36:10,580
+cells بيروح direct لوين؟ اللي هو gracile and
+
+467
+00:36:10,580 --> 00:36:14,730
+cuneate tractبعد هيك بيمر في الـ posterior limb في
+
+468
+00:36:14,730 --> 00:36:17,530
+الـ posterior column of spinal cord يكون الجرسيل
+
+469
+00:36:17,530 --> 00:36:20,890
+tracked و الكونية tracked و الموجودين في ال
+
+470
+00:36:20,890 --> 00:36:25,810
+posterior column of spinal cord بيصعدوا على نفس ال
+
+471
+00:36:25,810 --> 00:36:29,530
+side without decassation و بيعملوا relay و بيسلموا
+
+472
+00:36:29,530 --> 00:36:32,710
+الإشارة ل ال second order نيرون في الميدالة
+
+473
+00:36:32,710 --> 00:36:38,690
+أبلانجاتة اسمها Gersel and Konet nucleiSecond
+
+474
+00:36:38,690 --> 00:36:42,270
+Order Neurons هما Gracel and Cuneth نوكلياي
+
+475
+00:36:42,270 --> 00:36:45,430
+الموجودة في الميدالة أبلانجاتا بيحدثوا بعدها
+
+476
+00:36:45,430 --> 00:36:48,550
+Decassation على ال Opposite Side في الميدالة
+
+477
+00:36:48,550 --> 00:36:52,030
+أبلانجاتا بيروحوا ل Media Luminiscus وبعد ذلك
+
+478
+00:36:52,030 --> 00:36:55,850
+بيصعدوا في Pons من ال brain و بيعملوا relay win في
+
+479
+00:36:55,850 --> 00:36:59,980
+الثلمس يبقى أن الثلمس يعتبرthird order neuron هو
+
+480
+00:36:59,980 --> 00:37:03,460
+نفس ال pain و ال temperature sensation لاحظوا
+
+481
+00:37:03,460 --> 00:37:06,340
+انتوا ال first و ال third بيشبهوا بعض فيهم
+
+482
+00:37:06,340 --> 00:37:10,480
+الاختلاف دايما راح يكون في second order neuron ال
+
+483
+00:37:10,480 --> 00:37:14,540
+axon ascend to pass through the posterior limb of
+
+484
+00:37:14,540 --> 00:37:20,200
+internal capsule و بيمروا في الthalamocortical
+
+485
+00:37:20,200 --> 00:37:24,330
+tractلحد ما يوصل الإشارة لـ Cortical somatic
+
+486
+00:37:24,330 --> 00:37:29,650
+sensory area in the postcentral gyrus area 123 in
+
+487
+00:37:29,650 --> 00:37:37,850
+parietal loop فهذا شرحنا هاي ال tract بيطلع ال
+
+488
+00:37:37,850 --> 00:37:39,190
+fibers ل
+
+489
+00:37:41,940 --> 00:37:45,700
+مع ال nerve لل first order نيرون بوستيرو دجانجليون
+
+490
+00:37:45,700 --> 00:37:51,180
+بيمروا ل البوستيرو كولوم بيصعدوا في ال tract اما
+
+491
+00:37:51,180 --> 00:37:54,780
+gracele او اما conate tract و بيروحوا ل gracele
+
+492
+00:37:54,780 --> 00:37:58,720
+نوكلياس و conate نوكلياس بعدها ال second order
+
+493
+00:37:58,720 --> 00:38:01,580
+نيرون ال fiber يستعمل ال decassation و بتصعد على
+
+494
+00:38:01,580 --> 00:38:04,380
+ال opposite side في media lemniscus و بيروح ل
+
+495
+00:38:04,380 --> 00:38:08,880
+third order نيرون و منها ل ال area 123أنا بدي أحرك
+
+496
+00:38:08,880 --> 00:38:14,020
+الـ joint بتاعي أو fine touch او حركت ال ankle
+
+497
+00:38:14,020 --> 00:38:18,820
+joint الإشارة بتروح عن ال nerveعال first order
+
+498
+00:38:18,820 --> 00:38:21,400
+نيرون بصيروا الجانجليون بتفوت في ال spinal cord
+
+499
+00:38:21,400 --> 00:38:24,940
+ازي جراسيلي track تصعد عال second order نيرون
+
+500
+00:38:24,940 --> 00:38:27,680
+جراسيلي نوكلياس تعمل decassation عال media
+
+501
+00:38:27,680 --> 00:38:31,500
+lemniscus و بتصعد عال third order نيرون و بتسلم
+
+502
+00:38:31,500 --> 00:38:35,960
+الإشارة ل ال boss central gyrus area 123
+
+503
+00:38:47,620 --> 00:38:51,680
+هي واضح اندي اخر شئ هنا الفرق بينهم هذا اللون
+
+504
+00:38:51,680 --> 00:38:55,360
+الأزرق اللي لكيه هذا اللي هو pinion temperature
+
+505
+00:38:55,360 --> 00:38:58,660
+sensation و ال pinion هذا يعتبر deep sensation
+
+506
+00:38:58,660 --> 00:39:02,220
+لاحظوا انه ال first order نفس الشيء في بوستيروت
+
+507
+00:39:02,220 --> 00:39:05,620
+جانجليونوالـ third order نفس الشيء ثالمس الاختلاف
+
+508
+00:39:05,620 --> 00:39:08,200
+في ال second order أن ال fibers ما دخلت على
+
+509
+00:39:08,200 --> 00:39:11,240
+البوستيرو هورنسرز صعدت في ال white matter as
+
+510
+00:39:11,240 --> 00:39:14,660
+gracile or conate tract ويرحوا لل second order في
+
+511
+00:39:14,660 --> 00:39:18,140
+المنضلة أبلانجاتا إما conate أو gracile نوكلياي
+
+512
+00:39:18,140 --> 00:39:21,100
+وبعدها عملوا crossing على ال opposite side as
+
+513
+00:39:21,100 --> 00:39:26,660
+media luminescus وبيكملوا نفس الطريق ال gracile
+
+514
+00:39:26,660 --> 00:39:32,700
+tractموجود مر ميديا مشراحته موجود مر ميديا مشراحته
+
+515
+00:39:32,700 --> 00:39:32,860
+موجود مر ميديا مشراحته موجود مر ميديا مشراحته
+
+516
+00:39:32,860 --> 00:39:34,040
+موجود مر ميديا مشراحته موجود مر ميديا مشراحته
+
+517
+00:39:34,040 --> 00:39:34,260
+موجود مر ميديا مشراحته موجود مر ميديا مشراحته
+
+518
+00:39:34,260 --> 00:39:36,660
+موجود مر ميديا مشراحته موجود مر ميديا مشراحته
+
+519
+00:39:36,660 --> 00:39:37,940
+موجود مر ميديا مشراحته موجود مر ميديا مشراحته
+
+520
+00:39:37,940 --> 00:39:38,100
+موجود مر ميديا مشراحته موجود مر ميديا مشراحته
+
+521
+00:39:38,100 --> 00:39:44,340
+موجود مر ميديا مشراحته موجود
+
+522
+00:39:44,340 --> 00:39:49,380
+مر ميديا
+
+523
+00:39:49,380 --> 00:39:54,100
+مشراحته موجود
+
+524
+00:39:54,100 --> 00:40:00,190
+مر ميديا مشراحته موجودنفس الشيء grassy ال nucleus
+
+525
+00:40:00,190 --> 00:40:04,770
+و connect nucleus grassy ال nucleus موجودة more
+
+526
+00:40:04,770 --> 00:40:08,750
+medial و connect nucleus موجودة more lateral و
+
+527
+00:40:08,750 --> 00:40:12,710
+لهذا ال slide بكون أنا أنهيت لكم موضوع ال somatic
+
+528
+00:40:12,710 --> 00:40:16,610
+sensation وشرحنا ال pathway of somatic sensation
+
+529
+00:40:16,610 --> 00:40:21,090
+for pain, temperature, touch sensation and deep
+
+530
+00:40:21,090 --> 00:40:24,370
+sensation يعطيكوا الف عافية و دمتم بيه الف عافية
+
diff --git a/PL9fwy3NUQKwZ1Q7zOg1ObgufPMjRI3WY1/106ueYAKuA4.srt b/PL9fwy3NUQKwZ1Q7zOg1ObgufPMjRI3WY1/106ueYAKuA4.srt
new file mode 100644
index 0000000000000000000000000000000000000000..d003d53a8884d8ce77a980cea64f12bffb0817df
--- /dev/null
+++ b/PL9fwy3NUQKwZ1Q7zOg1ObgufPMjRI3WY1/106ueYAKuA4.srt
@@ -0,0 +1,770 @@
+1
+00:00:00,180 --> 00:00:04,740
+الآن شاء الله بدنا نحكي فيه الفحوصات المستخدمة
+
+2
+00:00:04,740 --> 00:00:09,720
+للكشف عن الأحماض الأمينية qualitative test of 
+
+3
+00:00:09,720 --> 00:00:15,040
+amino acid بدنا نحكي عن الفحوصات النوعية المستخدمة
+
+4
+00:00:15,040 --> 00:00:19,640
+للكشف عن الأحماض الأمينية qualitative تعني نوعية
+
+5
+00:00:20,300 --> 00:00:24,480
+يعني هذه الفحوصات أو النتيجة اللي هتظهر عندي هتكون
+
+6
+00:00:24,480 --> 00:00:29,440
+عبارة عن positive أو negative يوجد أو لا يوجد
+
+7
+00:00:29,440 --> 00:00:35,940
+qualitative معناها فحوصات نوعية أو جودة تعطي نتيجة
+
+8
+00:00:35,940 --> 00:00:41,640
+positive أو negative ظهور لون أو عدم ظهور لون، لا 
+
+9
+00:00:41,640 --> 00:00:45,140
+تعطي رقم في عندنا نوعتان من الفحوصات اللي هو
+
+10
+00:00:45,140 --> 00:00:51,030
+quantitative test Quantitative معناها كمية تحليل كم
+
+11
+00:00:51,030 --> 00:00:55,670
+يعني بديطلع عندك كمية أو مقدار يعني هيطلع عندك في
+
+12
+00:00:55,670 --> 00:00:59,850
+الآخر عدد تركيز بس اللي احنا هنتعرف عليه اليوم هو
+
+13
+00:00:59,850 --> 00:01:06,210
+qualitative test for amino acid فحوصات نوعية للكشف
+
+14
+00:01:06,210 --> 00:01:12,950
+عن الأحماض الأمينية في البداية نحكي مقدمة عن الأحماض
+
+15
+00:01:12,950 --> 00:01:17,130
+الأمينية و هذه الأشياء حكيناها في المعمل الماضي
+
+16
+00:01:17,130 --> 00:01:21,850
+حكينا إن الطعام ينقسم إلى ثلاثة إلى carbohydrate و
+
+17
+00:01:21,850 --> 00:01:25,270
+lipid و protein حكينا ال carbohydrate هو مصدر
+
+18
+00:01:25,270 --> 00:01:29,470
+الطاقة ال lipid هو مخزن الطاقة و ال protein هو
+
+19
+00:01:29,470 --> 00:01:34,250
+طاقة الخلايا لبناء الخلايا و للتجديد حكيناها المرة 
+
+20
+00:01:34,250 --> 00:01:39,900
+الماضية برضه حكينا عن الأحماض الأمينية إنه هي عبارة
+
+21
+00:01:39,900 --> 00:01:44,180
+عن الوحدة البنائية للبروتين وحكينا إنها بترتبط مع 
+
+22
+00:01:44,180 --> 00:01:47,680
+بعض بـ peptide bond حكينا الصيغة العامة للأحماض 
+
+23
+00:01:47,680 --> 00:01:51,240
+الأمينية عبارة عن H وإن كل حمض أمين بيختلف عن
+
+24
+00:01:51,240 --> 00:01:57,580
+الآخر بالـ R الـ side الـ chain حكينا كمان المرة
+
+25
+00:01:57,580 --> 00:01:59,780
+الماضية إن الأحماض الأمينية
+
+26
+00:02:03,760 --> 00:02:06,880
+وين بتيجي الرابطة اللي بتبدأها؟ بين مجموعة كربوكسيل
+
+27
+00:02:06,880 --> 00:02:11,700
+من حمض أميني ومجموعة أمين من حمض أميني آخر يتم نزع
+
+28
+00:02:11,700 --> 00:02:15,960
+جزء ماء في هذه العملية فسمينا التفاعل تكون
+
+29
+00:02:15,960 --> 00:02:20,800
+الرابطة اللي بتبدأها بـ Condensation Reaction لأنه
+
+30
+00:02:20,800 --> 00:02:24,500
+بينتج عنها ماء حكينا على الأطراف بيظل عند Free
+
+31
+00:02:24,500 --> 00:02:28,920
+Amino Group بنسميها الـ N Terminus و Free Carboxyl
+
+32
+00:02:28,920 --> 00:02:35,040
+Group سميناها الـ C Terminus هنا كمان إن الأحماض
+
+33
+00:02:35,040 --> 00:02:39,000
+الأمينية تنقسم حسب المصدر إلى essential و non
+
+34
+00:02:39,000 --> 00:02:43,320
+-essential ال essential الجسم غير قادر على تصنيعها
+
+35
+00:02:43,320 --> 00:02:47,040
+يجب توفيرها في الوجبات ال non-essential الجسم
+
+36
+00:02:47,040 --> 00:02:51,420
+قادر على تصنيعها فمش ضروري تكون موجودة في الوجبات
+
+37
+00:02:51,420 --> 00:02:58,610
+اليومية و هنا حكينا أن أبسط حمض أميني هو ال glycine
+
+38
+00:02:58,610 --> 00:03:02,590
+ليش؟ لأن الـ side-chain أو الـ R-group هي عبارة عن
+
+39
+00:03:02,590 --> 00:03:06,730
+ذرة هيدروجين فقط و حكينا كمان أن الحمض الأمين
+
+40
+00:03:06,730 --> 00:03:10,990
+الشاذ أو الفريد باختلف عن غيره من الأحماض الأمينية
+
+41
+00:03:10,990 --> 00:03:16,430
+هو البرولين لأنه ما عندهوش amino group حرة اللي 
+
+42
+00:03:16,430 --> 00:03:19,890
+متصلة في الـ central carbon سميناها primary amino
+
+43
+00:03:19,890 --> 00:03:24,530
+group في كل الأحماض الأمينية تسمى مجموعة الـ amino
+
+44
+00:03:24,530 --> 00:03:30,030
+group هي primary amino group أو free alpha amino
+
+45
+00:03:30,030 --> 00:03:35,030
+group إلا في حالة البرولين تسمى secondary amino
+
+46
+00:03:35,030 --> 00:03:39,270
+group أو amino group لأنها متصلة بالـ central
+
+47
+00:03:39,270 --> 00:03:47,470
+carbon من جهة بالـ side chain من جهة أخرى حكينا عن 
+
+48
+00:03:47,470 --> 00:03:51,670
+الأحماض الأمينية وتقسيمها حسب ال polarity إلى polar
+
+49
+00:03:51,670 --> 00:03:53,050
+و non polar
+
+50
+00:03:56,470 --> 00:04:00,650
+charge و ال charge في منها positive charge و في
+
+51
+00:04:00,650 --> 00:04:04,790
+منها negative charge فهذه التقسيمة كلها سواء polar
+
+52
+00:04:04,790 --> 00:04:09,510
+أو non polar تعتمد على الـ side chain بتطلع على الـ
+
+53
+00:04:09,510 --> 00:04:13,430
+side chain حكينا حكي إن إذا في بس كربون و هيدروجين
+
+54
+00:04:13,430 --> 00:04:18,930
+بتكون non polar إذا فيها مجموعات قطبية بتكون polar
+
+55
+00:04:18,930 --> 00:04:22,670
+بتطلع إذا كانت مشحونة بتكون charge و إذا غير
+
+56
+00:04:22,670 --> 00:04:25,390
+مشحونة بتكون uncharged
+
+57
+00:04:27,110 --> 00:04:31,130
+حكينا عن هذه الصورة اللي بتقسم للأحماض الأمينية
+
+58
+00:04:31,130 --> 00:04:35,610
+إلى non-polar و polar ال polar في منها uncharged و
+
+59
+00:04:35,610 --> 00:04:39,950
+في منها charged و في positive و في negative حكينا
+
+60
+00:04:39,950 --> 00:04:43,510
+في أحماض أمينية بنسميها aromatic amino acid
+
+61
+00:04:43,510 --> 00:04:47,070
+aromatic اللي هي بتحتوي في الـ side chain على حلقة
+
+62
+00:04:47,070 --> 00:04:50,650
+بنزين إذا كان في الـ side chain في حلقة بنسميها
+
+63
+00:04:50,650 --> 00:04:53,290
+aromatic amino acid
+
+64
+00:04:56,910 --> 00:05:00,770
+ويجي نحكي عن حاجة اسمها Ionization Ionization
+
+65
+00:05:00,770 --> 00:05:05,110
+معناها كيف يتصرف الحمض الأميني في الأوساط
+
+66
+00:05:05,110 --> 00:05:10,190
+المختلفة، في وسط قاعدي، في وسط حمضي، في neutral، 
+
+67
+00:05:10,190 --> 00:05:15,330
+في pH، في وسط المتعادل لو جينا و اتفرج على
+
+68
+00:05:15,330 --> 00:05:19,210
+المعادلة هاي اللي فوق في الوسط هذا حمض أميني موجود
+
+69
+00:05:19,210 --> 00:05:24,350
+في وسط متعادل الصيغة العامة لحمض أميني موجود في وسط
+
+70
+00:05:24,350 --> 00:05:29,790
+متعادل بتكون عدد الشحنات الموجبة بتساوي عدد الشحنات
+
+71
+00:05:29,790 --> 00:05:33,690
+السالبة إن هنا مكتوب CH2 هو الصيغة العامة للحمض
+
+72
+00:05:33,690 --> 00:05:38,150
+الأميني Central Carbon و Hydrogen و الـ R group هي
+
+73
+00:05:38,150 --> 00:05:41,250
+عبارة عن ذرة الهيدروجين المعتبرة هنا وإنقدش
+
+74
+00:05:41,250 --> 00:05:45,670
+NH3 plus و COO minus هي الصيغة العامة للحمض
+
+75
+00:05:45,670 --> 00:05:49,090
+الأميني في الوسط المتعادل تكون الشحنات الموجبة
+
+76
+00:05:49,090 --> 00:05:54,830
+بتساوي الشحنات السالبة الـ net charge بتكون
+
+77
+00:05:54,830 --> 00:05:59,330
+زيرو لو جيت أخدت هذا الحمض الأميني حطيته في وسط
+
+78
+00:05:59,330 --> 00:06:07,010
+حمضي وسط حمضي يعني قيمة الـ pH يعني أنه كان فيه
+
+79
+00:06:07,010 --> 00:06:11,130
+زيادة في أيونات الهيدروجين يعني هذا الوسط مليء بـ
+
+80
+00:06:11,130 --> 00:06:16,620
+H plus زيادة فالحمض الأميني دخله من المتعادل وحطيته
+
+81
+00:06:16,620 --> 00:06:20,120
+في وسط الحمضي وسط الحمضي فيه زيادة في أيونات
+
+82
+00:06:20,120 --> 00:06:24,400
+الهيدروجين الـ NH3  بده يحتاج الهيدروجين هياخد من
+
+83
+00:06:24,400 --> 00:06:28,920
+الوسط COO- محتاج الهيدروجين فبتستحب من الوسط
+
+84
+00:06:28,920 --> 00:06:37,990
+هيدروجين و بتتشبع بـ CRCOOH فبتصير CH2 COOH NH3 Plus
+
+85
+00:06:37,990 --> 00:06:44,990
+بصير الشكل العام له CH2 COOH NH3 Plus فأخذ ذرة
+
+86
+00:06:44,990 --> 00:06:49,960
+هيدروجينية صارت الـ net charge عليه positive charge اللي
+
+87
+00:06:49,960 --> 00:06:53,040
+موجودة في المعادلة على اليسار هذا اللي هو الصيغة
+
+88
+00:06:53,040 --> 00:06:59,480
+العامة الحمض الأميني في وسط حمضي CH2COHNH3Plus
+
+89
+00:06:59,480 --> 00:07:03,580
+إن الكربوكسيل أخدت أيون هيدروجين أو بروتون
+
+90
+00:07:03,580 --> 00:07:08,720
+هيدروجين من الوسط الحمضي اللي هي فيه فصارت COH net
+
+91
+00:07:08,720 --> 00:07:13,960
+charge في الوسط الحمضي صارت Positive charge طبعا لو
+
+92
+00:07:13,960 --> 00:07:18,060
+أخدتها الوسط من الوسط المتعادل وحطيته في وسط قاعدي
+
+93
+00:07:18,060 --> 00:07:25,280
+الوسط القاعدي مليء بـ OH- وسط بيكون مليء بـ OH- OH
+
+94
+00:07:25,280 --> 00:07:30,140
+- هي تحتاج إلى ذرة هيدروجين عشان تتكون جزئنا 
+
+95
+00:07:30,140 --> 00:07:35,920
+فبتروح على أي ذرة جزئية بتنزع منه هيدروجين عشان
+
+96
+00:07:35,920 --> 00:07:40,320
+تتكوننا من عندنا بنقدر ننزع منه هيدروجين لأن NH3
+
+97
+00:07:40,320 --> 00:07:46,960
+plus تاخد منه هيدروجين فبصير NH2 COO minus هتتغير؟
+
+98
+00:07:46,960 --> 00:07:52,300
+لا مش هتتغير فالوسط المتعادل CH2 NH3 plus COO minus
+
+99
+00:07:52,300 --> 00:07:57,360
+لما يروح على القاعدة اللي هي على اليمين بيصير NH2
+
+100
+00:07:57,360 --> 00:08:02,860
+و COO minus اتغيرت من الـ NH3 نزعنا منها هيدروجين
+
+101
+00:08:02,860 --> 00:08:07,600
+و صارت NH2 الـ net charge إيه لو بيكون minus ففي
+
+102
+00:08:07,600 --> 00:08:11,920
+حالة الوسط الحمضي .. في حالة وسط متعادل الـ net
+
+103
+00:08:11,920 --> 00:08:16,360
+charge بتكون zero في حالة وسط حمضي بيكون الـ net
+
+104
+00:08:16,360 --> 00:08:20,100
+charge positive في حالة الوسط القاعدي الـ net
+
+105
+00:08:20,100 --> 00:08:24,700
+charge negative هلجد؟ الحمض الأميني اللي بتكون
+
+106
+00:08:24,700 --> 00:08:29,120
+متساوي عليه الشحنات الموجبة و الشحنات السالبة و الـ
+
+107
+00:08:29,120 --> 00:08:32,960
+net charge بتكون بتساوي zero بسميه Zwitterion
+
+108
+00:08:32,960 --> 00:08:37,580
+Zwitterion هو الحمض الأميني اللي تتساوي عليه
+
+109
+00:08:37,580 --> 00:08:42,180
+الشحنة الموجبة و الشحنة السالبة يعني الشحنة الكلية
+
+110
+00:08:42,180 --> 00:08:46,280
+تكون صفر وين موجود الـ Zwitterion؟ في الوسط
+
+111
+00:08:46,280 --> 00:08:50,650
+المتعادل كان موجود بالوسط المتعادل الحمض الأميني
+
+112
+00:08:50,650 --> 00:08:54,790
+بيكون الشحن الموجبة بتساوي الشحن السالبة يعني الشحن
+
+113
+00:08:54,790 --> 00:08:59,950
+الكلية بتساوي صفر وهذا بسميه Zwitterion Zwitterion
+
+114
+00:08:59,950 --> 00:09:03,870
+هو الحمض الأميني الذي تتساوي عليه الشحن الموجبة
+
+115
+00:09:03,870 --> 00:09:10,520
+والشحن السالبة و net charge Zero عند الوسط المتعادل هذا،
+
+116
+00:09:10,520 --> 00:09:13,240
+عند تكون الحمض الأميني اللي بتكون شحنات الموجبة
+
+117
+00:09:13,240 --> 00:09:16,340
+السالبة بتساوي الموجبة، في حاجة بنتسميها
+
+118
+00:09:16,340 --> 00:09:21,100
+isoelectric point أو الـ pH الـ pI أو الـ 
+
+119
+00:09:21,100 --> 00:09:26,540
+isoelectric point هي قيمة pH بتتساوي عندها الشحن
+
+120
+00:09:26,540 --> 00:09:32,320
+الموجبة والسالبة على سطح الحمض الأميني وبالتالي، 
+
+121
+00:09:32,320 --> 00:09:38,620
+شحنة الكلية صفر قيمة pH تتساوى عندها شحنة الموجبة
+
+122
+00:09:38,620 --> 00:09:43,760
+والسالبة على سطح الحمض الأمين وبالتالي، شحنة
+
+123
+00:09:43,760 --> 00:09:47,640
+الكلية بتساوي صفر هاي الـ Isoelectric point، 
+
+124
+00:09:47,640 --> 00:09:53,260
+الاختصار بـ pI تمام، في حاجة بنسميها protonated
+
+125
+00:09:53,260 --> 00:09:57,740
+معناها إنه اكتساب بروتون أو H plus في حاجة بنسميها
+
+126
+00:09:57,740 --> 00:10:02,780
+Deprotonated يعني فقد بروتون اللي بياخد H plus يعني 
+
+127
+00:10:02,780 --> 00:10:07,800
+في الوسط الحمضي زي COO minus اكتسبت H فهذه gain of
+
+128
+00:10:07,800 --> 00:10:12,800
+البروتون يعني صارلها protonated Deprotonated يعني
+
+129
+00:10:12,800 --> 00:10:16,760
+loss of البروتون زي الـ NH3 plus في الوسط القاعدة
+
+130
+00:10:16,760 --> 00:10:22,060
+لما حضيتها صارت NH2 نزعت منها H فبسميها .. بقول
+
+131
+00:10:22,060 --> 00:10:26,600
+عنها نصارلها Deprotonated فقدت البروتون
+
+132
+00:10:30,550 --> 00:10:34,490
+هنا بيحكي لي أن الـ Isoelectric point أو الـ PI هي
+
+133
+00:10:34,490 --> 00:10:39,090
+قيمة pH اللي بيكون فيها الـ anionic بتساوي الـ cationic
+
+134
+00:10:39,090 --> 00:10:45,350
+الـ net charge بتكون Zero كل حمض أميني له PI أو
+
+135
+00:10:45,350 --> 00:10:49,490
+Isoelectric point بتختلف عن غيره حسب شو؟ حسب الـ
+
+136
+00:10:49,490 --> 00:10:52,830
+side chain فهو حاكي أن كل حمض أميني بيميزه عن غيره
+
+137
+00:10:52,830 --> 00:10:54,110
+side chain
+
+138
+00:10:56,690 --> 00:11:01,790
+نحكي في الجزء العملي للمعمل الأول جزء العملي بيحكي
+
+139
+00:11:01,790 --> 00:11:06,930
+عن qualitative test for amino acid فحوصات جودة
+
+140
+00:11:06,930 --> 00:11:10,710
+للكشف عن الأحماض الأمينية النتيجة عندنا هتكون
+
+141
+00:11:10,710 --> 00:11:16,330
+positive أو negative «ظهور لون» أو «عدم ظهور لون»
+
+142
+00:11:16,330 --> 00:11:19,450
+في عندنا number of tests to detect the presence of
+
+143
+00:11:19,450 --> 00:11:23,210
+amino acid هذه الفحوصات الكاشفة عن الأحماض الأمينية
+
+144
+00:11:23,210 --> 00:11:26,830
+largely depend on the nature of side chain علشان
+
+145
+00:11:26,830 --> 00:11:29,770
+بيعتمدوا على الـ side chain لأنه حكي إن اللي بيميز
+
+146
+00:11:29,770 --> 00:11:34,090
+حمض أمين عن الآخر هو الـ side chain الجد إحنا
+
+147
+00:11:34,090 --> 00:11:38,210
+الفحوصات اللي هنشتغلها بيطلع معنا لون يتغير أو
+
+148
+00:11:38,210 --> 00:11:44,060
+ما يتغير هذا اللون له شدة intensity of color شدة
+
+149
+00:11:44,060 --> 00:11:47,940
+اللون هي بتكون تختلف يعني ممكن هو يتلون بس على
+
+150
+00:11:47,940 --> 00:11:52,580
+درجات من حاجة لتانية تكون درجة بتختلف شدة اللون هي
+
+151
+00:11:52,580 --> 00:11:56,860
+علشان بتعتمد بتعتمد على أول شيء number of reacting
+
+152
+00:11:56,860 --> 00:12:00,900
+unit number of reacting unit اللي هي الـ reacting
+
+153
+00:12:00,900 --> 00:12:05,040
+unit هي عبارة عن المادة الفعالة قد إيش عدد المادة 
+
+154
+00:12:05,040 --> 00:12:08,780
+الفعالة الموجودة؟ إيش هي المادة الفعالة؟ دي الـ شيء 
+
+155
+00:12:08,780 --> 00:12:12,940
+اللي بدور عليها الفحص أنا بشتغل فحص مثلا بيدور على
+
+156
+00:12:12,940 --> 00:12:15,980
+peptide bond إذا موجودة الـ peptide bond، يعطيني
+
+157
+00:12:15,980 --> 00:12:19,900
+لون إذا مش موجودة، مش هيتغير اللون طب الـ peptide
+
+158
+00:12:19,900 --> 00:12:23,160
+bond هذه ممكن تكون بس رابطتين، ويعطيني لون ممكن 
+
+159
+00:12:23,160 --> 00:12:26,400
+تكون ثلاث ويعطيني لون بس هل اللون هيكون نفسه إذا
+
+160
+00:12:26,400 --> 00:12:30,080
+كانوا اثنتين وإذا كانوا ثلاث؟ شدة اللون هتختلف
+
+161
+00:12:30,080 --> 00:12:34,810
+بناء على number of reacting unit الحاجة الثانية
+
+162
+00:12:34,810 --> 00:12:37,890
+اللي بتعتمد عليها شدة اللون هي الـ accessibility
+
+163
+00:12:37,890 --> 00:12:42,050
+to reaction هل هذه المادة الفعالة الـ reacting unit
+
+164
+00:12:42,050 --> 00:12:46,350
+قريبة من التفاعل، هل هي حرة، free، بتقدر تيجي
+
+165
+00:12:46,350 --> 00:12:48,990
+ترتبط، تشتغل مع التفاعل، مع الـ agent اللي أنا
+
+166
+00:12:48,990 --> 00:12:52,910
+حطيته، تشتغل و تتغير اللون، ولا إنها مشغولة، مش
+
+167
+00:12:52,910 --> 00:12:56,930
+هتقدر في حواجز، مش هتقدر تيجي ترتبط هي بتسميها
+
+168
+00:12:56,930 --> 00:13:00,810
+accessibility to reaction إذا تشدت اللون تعتمد على
+
+169
+00:13:00,810 --> 00:13:04,450
+حاجتين على الـ number of reacting unit وعلى
+
+170
+00:13:04,450 --> 00:13:10,470
+accessibility to reaction إيش 
+
+171
+00:13:10,470 --> 00:13:13,570
+هي الفحوصات اللي هنستخدمها للكشف عن الأحماض
+
+172
+00:13:13,570 --> 00:13:19,070
+الأمينية؟ هنا فحص اسمه ninhydrin test for 3-alpha
+
+173
+00:13:19,070 --> 00:13:24,160
+amino group على Free Alpha Amino Group إذا موجودة
+
+174
+00:13:24,160 --> 00:13:27,280
+بيتغير اللون وبيعطي positive إذا مش موجودة مش
+
+175
+00:13:27,280 --> 00:13:30,880
+هيتغير اللون فالـ Free Alpha Amino Group هاي
+
+176
+00:13:30,880 --> 00:13:35,000
+بسميها الـ Reacting Unit فحص الثاني اسمه Bio-Red
+
+177
+0
+
+201
+00:15:18,530 --> 00:15:23,350
+هاي سميناها primary amino group أو alpha
+
+202
+00:15:23,350 --> 00:15:27,530
+amino group معادة في حالة البرولين سميناها amino 
+
+203
+00:15:27,530 --> 00:15:32,840
+groupيعني الأحماض الأمينية كلها تحتوي على free
+
+204
+00:15:32,840 --> 00:15:37,700
+alpha amino group ما عدا البرولين عنده amino group
+
+205
+00:15:37,700 --> 00:15:40,760
+يعني مين هيعطيني positive مع هذا الفحص لو أنا
+
+206
+00:15:40,760 --> 00:15:44,440
+اشتغلت مجموعة أحماض أمينية على هذا الفحص؟ كلهم
+
+207
+00:15:44,440 --> 00:15:47,540
+هيعطوني positive لأنهم كلهم بيحتوي على free alpha
+
+208
+00:15:47,540 --> 00:15:53,040
+amino group تمام؟ طيب، إذا ال objective to detect
+
+209
+00:15:53,040 --> 00:15:55,520
+the presence of free alpha amino group هل موجود
+
+210
+00:15:55,520 --> 00:15:58,420
+free alpha amino group أو لا؟ إذا تغير اللون يعني
+
+211
+00:15:58,420 --> 00:16:01,560
+موجود، إذا ما تغيرش يعني مش موجود طيب إيش ال
+
+212
+00:16:01,560 --> 00:16:05,620
+principle؟ مبتدأ العمل للفحص؟ كيف يشتغل الفحص؟ ال
+
+213
+00:16:05,620 --> 00:16:08,520
+principle لو نعملها على نظام معادلات بيسهل عليه
+
+214
+00:16:08,520 --> 00:16:12,380
+كيف هذا الفحص له ال principle؟ إنه عندنا amino
+
+215
+00:16:12,380 --> 00:16:15,940
+acid أنا بضيف حمض أميني، بضيف عليه نين هيدرين ريأشا 
+
+216
+00:16:15,940 --> 00:16:17,240
+إيش بيعمل ال 
diff --git a/PL9fwy3NUQKwZ1Q7zOg1ObgufPMjRI3WY1/106ueYAKuA4_raw.json b/PL9fwy3NUQKwZ1Q7zOg1ObgufPMjRI3WY1/106ueYAKuA4_raw.json
new file mode 100644
index 0000000000000000000000000000000000000000..9fb8cc39a3536c3f32274c2d1b56f9ae611a3331
--- /dev/null
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@@ -0,0 +1 @@
+{"segments": [{"id": 1, "seek": 1964, "start": 0.18, "end": 19.64, "text": "الان شاء الله بدنا نحكي فيه الفحوصات المستخدمة للكشف عن الأحماض الأمينية qualitative test of amino acid بدنا نحكي عن الفحوصات النوعية المستخدمة للكشف عن الأحماض الأمينية qualitative تعني نوعية", "tokens": [6027, 7649, 13412, 16606, 21984, 47525, 8315, 8717, 5016, 4117, 1829, 8978, 3224, 27188, 5016, 2407, 9381, 9307, 9673, 14851, 9778, 40448, 3660, 5296, 23275, 8592, 5172, 18871, 16247, 35571, 46958, 16247, 2304, 9957, 10632, 31312, 1500, 295, 24674, 8258, 47525, 8315, 8717, 5016, 4117, 1829, 18871, 27188, 5016, 2407, 9381, 9307, 28239, 45367, 10632, 9673, 14851, 9778, 40448, 3660, 5296, 23275, 8592, 5172, 18871, 16247, 35571, 46958, 16247, 2304, 9957, 10632, 31312, 37279, 22653, 8717, 45367, 10632], "avg_logprob": -0.10700158095812495, "compression_ratio": 2.0, "no_speech_prob": 0.0003075599670410156, "words": [{"start": 0.18, "end": 0.62, "word": "الان", "probability": 0.465576171875}, {"start": 0.62, "end": 0.88, "word": " شاء", "probability": 0.915283203125}, {"start": 0.88, "end": 1.18, "word": " الله", "probability": 0.9619140625}, {"start": 1.18, "end": 1.56, "word": " بدنا", "probability": 0.49072265625}, {"start": 1.56, "end": 2.06, "word": " نحكي", "probability": 0.9732666015625}, {"start": 2.06, "end": 2.62, "word": " فيه", "probability": 0.977294921875}, {"start": 2.62, "end": 3.78, "word": " الفحوصات", "probability": 0.9671875}, {"start": 3.78, "end": 4.74, "word": " المستخدمة", "probability": 0.975390625}, {"start": 4.74, "end": 5.32, "word": " للكشف", "probability": 0.9449462890625}, {"start": 5.32, "end": 5.58, "word": " عن", "probability": 0.98876953125}, {"start": 5.58, "end": 6.34, "word": " الأحماض", "probability": 0.8421223958333334}, {"start": 6.34, "end": 7.14, "word": " الأمينية", "probability": 0.94873046875}, {"start": 7.14, "end": 8.12, "word": " qualitative", "probability": 0.8759765625}, {"start": 8.12, "end": 9.08, "word": " test", "probability": 0.6748046875}, {"start": 9.08, "end": 9.72, "word": " of", "probability": 0.97021484375}, {"start": 9.72, "end": 10.4, "word": " amino", "probability": 0.87109375}, {"start": 10.4, "end": 11.0, "word": " acid", "probability": 0.9169921875}, {"start": 11.0, "end": 11.54, "word": " بدنا", "probability": 0.737060546875}, {"start": 11.54, "end": 12.04, "word": " نحكي", "probability": 0.99169921875}, {"start": 12.04, "end": 12.2, "word": " عن", "probability": 0.98974609375}, {"start": 12.2, "end": 13.02, "word": " الفحوصات", "probability": 0.99482421875}, {"start": 13.02, "end": 14.06, "word": " النوعية", "probability": 0.93505859375}, {"start": 14.06, "end": 15.04, "word": " المستخدمة", "probability": 0.9578125}, {"start": 15.04, "end": 15.46, "word": " للكشف", "probability": 0.971923828125}, {"start": 15.46, "end": 15.62, "word": " عن", "probability": 0.955078125}, {"start": 15.62, "end": 16.16, "word": " الأحماض", "probability": 0.9716796875}, {"start": 16.16, "end": 16.94, "word": " الأمينية", 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"probability": 0.9733072916666666}, {"start": 92.27, "end": 92.43, "word": " و", "probability": 0.72119140625}, {"start": 92.43, "end": 93.03, "word": " للتجد", "probability": 0.962158203125}, {"start": 93.03, "end": 93.99, "word": " حكيناها", "probability": 0.881103515625}, {"start": 93.99, "end": 94.25, "word": " المرة", "probability": 0.89013671875}, {"start": 94.25, "end": 94.73, "word": " الماضية", "probability": 0.8955078125}], "temperature": 1.0}, {"id": 5, "seek": 11978, "start": 95.58, "end": 119.78, "text": "برضه حكينا عن الأحماض الأمينية إنه هي عبارة عن الوحدة البنائية للبروتين وحكينا إنها بترتبط مع بعض بـprotein bond حكينا الصيغة العامة للأحماض الأمينية عبارة عن H وإن كل حمض أمين بيختلف عن الآخر بال R ال side ال chain حكينا كمان المرة الماضية إن الأحماض الأمينية", "tokens": [3555, 43042, 3224, 11331, 4117, 1829, 8315, 18871, 16247, 35571, 46958, 16247, 2304, 9957, 10632, 36145, 3224, 39896, 6225, 3555, 9640, 3660, 18871, 2423, 2407, 24401, 3660, 29739, 1863, 16373, 10632, 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non-essential ال essential الجسم غير قادر على تصنيعها يجب توفيرها في الواجبات ال non-essential الجسم قادر على تصنيحها فمش ضرور تكون موجودة في الواجبات اليومية", "tokens": [3224, 8315, 9122, 2304, 7649, 36145, 16247, 35571, 46958, 16247, 27842, 10632, 6055, 1863, 4587, 38251, 11331, 35457, 9673, 9381, 3215, 2288, 30731, 7115, 4032, 2107, 12, 48143, 2423, 7115, 25724, 38251, 32771, 13546, 12174, 18513, 2288, 15844, 6055, 9381, 22653, 3615, 11296, 7251, 7435, 3555, 33427, 5172, 13546, 11296, 8978, 2423, 2407, 26108, 3555, 9307, 2423, 2107, 12, 48143, 25724, 38251, 12174, 18513, 2288, 15844, 6055, 9381, 22653, 5016, 11296, 6156, 2304, 8592, 48812, 2288, 13063, 6055, 30544, 3714, 29245, 23328, 3660, 8978, 2423, 2407, 26108, 3555, 9307, 45595, 20498, 10632], "avg_logprob": -0.0758988562450614, "compression_ratio": 1.8894736842105264, "no_speech_prob": 2.2649765014648438e-05, "words": [{"start": 153.04, "end": 153.38, "word": "هنا", "probability": 0.907470703125}, {"start": 153.38, "end": 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"probability": 0.94140625}, {"start": 517.58, "end": 518.1, "word": " الشحنة", "probability": 0.92041015625}, {"start": 518.1, "end": 518.64, "word": " الموجبة", "probability": 0.99609375}, {"start": 518.64, "end": 519.1, "word": " و", "probability": 0.95654296875}, {"start": 519.1, "end": 519.74, "word": " الشحنة", "probability": 0.9720458984375}, {"start": 519.74, "end": 520.54, "word": " السالبة", "probability": 0.9814453125}, {"start": 520.54, "end": 521.04, "word": " يعني", "probability": 0.920166015625}, {"start": 521.04, "end": 521.5, "word": " الشحنة", "probability": 0.9814453125}, {"start": 521.5, "end": 522.18, "word": " الكلية", "probability": 0.6494954427083334}, {"start": 522.18, "end": 523.02, "word": " تكون", "probability": 0.846923828125}, {"start": 523.02, "end": 523.52, "word": " صفر", "probability": 0.7974446614583334}, {"start": 523.52, "end": 524.12, "word": " وين", "probability": 0.780517578125}, {"start": 524.12, "end": 524.48, "word": " موجود", "probability": 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{"start": 556.34, "end": 557.66, "word": " isoelectric", "probability": 0.6396891276041666}, {"start": 557.66, "end": 558.1, "word": " point", "probability": 0.77099609375}, {"start": 558.1, "end": 558.72, "word": " أو", "probability": 0.79345703125}, {"start": 558.72, "end": 558.96, "word": " الـ", "probability": 0.873291015625}, {"start": 558.96, "end": 559.48, "word": " PR", "probability": 0.0787353515625}, {"start": 559.48, "end": 560.14, "word": " الـ", "probability": 0.76953125}, {"start": 560.14, "end": 560.58, "word": " PI", "probability": 0.7958984375}, {"start": 560.58, "end": 560.88, "word": " أو", "probability": 0.7548828125}, {"start": 560.88, "end": 561.1, "word": " الـ", "probability": 0.76025390625}, {"start": 561.1, "end": 561.84, "word": " isoelectric", "probability": 0.8419596354166666}, {"start": 561.84, "end": 562.4, "word": " point", "probability": 0.98046875}, {"start": 562.4, "end": 563.12, "word": " هي", "probability": 0.9013671875}, {"start": 563.12, "end": 564.1, "word": " قيمة", "probability": 0.9962565104166666}, {"start": 564.1, "end": 564.68, "word": " BH", "probability": 0.460205078125}, {"start": 564.68, "end": 565.76, "word": " بتتساوي", "probability": 0.84990234375}, {"start": 565.76, "end": 566.02, "word": " عند", "probability": 0.98046875}, {"start": 566.02, "end": 566.54, "word": " الشحن", "probability": 0.95263671875}, {"start": 566.54, "end": 567.22, "word": " الموجبة", "probability": 0.9845377604166666}, {"start": 567.22, "end": 568.6, "word": " والسالبة", "probability": 0.9437255859375}, {"start": 568.6, "end": 569.1, "word": " على", "probability": 0.84375}, {"start": 569.1, "end": 569.6, "word": " سطح", "probability": 0.9915364583333334}, {"start": 569.6, "end": 570.16, "word": " الحمض", "probability": 0.994140625}, {"start": 570.16, "end": 570.76, "word": " الأميني", "probability": 0.988037109375}], "temperature": 1.0}, {"id": 26, "seek": 60029, "start": 571.1, "end": 600.3, "text": "وبالتالي، شحنة الكلية صفر قيمة BH 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"-peptide", "probability": 0.7255401611328125}, {"start": 817.38, "end": 817.94, "word": " bond", "probability": 0.92138671875}, {"start": 817.94, "end": 818.74, "word": " لإنه", "probability": 0.61199951171875}, {"start": 818.74, "end": 819.1, "word": " فحص", "probability": 0.9563802083333334}, {"start": 819.1, "end": 819.24, "word": " ال", "probability": 0.7412109375}, {"start": 819.24, "end": 820.52, "word": "-xanthoproteic", "probability": 0.73577880859375}, {"start": 820.52, "end": 820.98, "word": " test", "probability": 0.8974609375}], "temperature": 1.0}, {"id": 36, "seek": 83866, "start": 821.42, "end": 838.66, "text": "Zanto Proteic Test for Aromatic Amino Acid لأ الأحماض الأمنية اللي تحتوي على حلقة بنزين نشطة نضيف هنا Activated Aromatic Amino Acid", "tokens": [57, 5857, 43371, 299, 9279, 337, 1587, 13143, 2012, 2982, 5097, 327, 5296, 10721, 16247, 35571, 46958, 16247, 27842, 10632, 13672, 1829, 6055, 33753, 45865, 15844, 11331, 1211, 28671, 44945, 11622, 9957, 8717, 8592, 9566, 3660, 8717, 11242, 33911, 34105, 28550, 770, 1587, 13143, 2012, 2982, 5097, 327], "avg_logprob": -0.21444515792690977, "compression_ratio": 1.2605633802816902, "no_speech_prob": 7.76052474975586e-05, "words": [{"start": 821.42, "end": 822.26, "word": "Zanto", "probability": 0.578369140625}, {"start": 822.26, "end": 823.3, "word": " Proteic", "probability": 0.71142578125}, {"start": 823.3, "end": 823.7, "word": " Test", "probability": 0.880859375}, {"start": 823.7, "end": 824.28, "word": " for", "probability": 0.6279296875}, {"start": 824.28, "end": 825.04, "word": " Aromatic", "probability": 0.742431640625}, {"start": 825.04, "end": 825.66, "word": " Amino", "probability": 0.95166015625}, {"start": 825.66, "end": 826.28, "word": " Acid", "probability": 0.744384765625}, {"start": 826.28, "end": 831.24, "word": " لأ", "probability": 0.6417236328125}, {"start": 831.24, "end": 831.84, "word": " الأحماض", "probability": 0.7781575520833334}, {"start": 831.84, "end": 832.44, "word": " الأمنية", "probability": 0.89404296875}, {"start": 832.44, "end": 832.58, "word": " اللي", "probability": 0.5924072265625}, {"start": 832.58, "end": 833.14, "word": " تحتوي", "probability": 0.8898111979166666}, {"start": 833.14, "end": 833.3, "word": " على", "probability": 0.87646484375}, {"start": 833.3, "end": 833.68, "word": " حلقة", "probability": 0.9576822916666666}, {"start": 833.68, "end": 834.28, "word": " بنزين", "probability": 0.9781901041666666}, {"start": 834.28, "end": 835.3, "word": " نشطة", "probability": 0.856201171875}, {"start": 835.3, "end": 835.94, "word": " نضيف", "probability": 0.83935546875}, {"start": 835.94, "end": 836.28, "word": " هنا", "probability": 0.8291015625}, {"start": 836.28, "end": 837.2, "word": " Activated", "probability": 0.783935546875}, {"start": 837.2, "end": 837.78, "word": " Aromatic", "probability": 0.930908203125}, {"start": 837.78, "end": 838.34, "word": " Amino", "probability": 0.959228515625}, {"start": 838.34, "end": 838.66, "word": " Acid", "probability": 0.961669921875}], "temperature": 1.0}, {"id": 37, "seek": 85572, "start": 839.5, "end": 855.72, "text": "هنا فحص اسمه hopkincol أو glycosilic acid reaction اللي هو اسمه hopkincol، glycosilic acid reaction بيدور على الـ Indole group الـ melons test for amino acid containing hydroxyl", "tokens": [3224, 8315, 6156, 5016, 9381, 24525, 2304, 3224, 3818, 74, 4647, 401, 34051, 22633, 6877, 388, 299, 8258, 5480, 13672, 1829, 31439, 24525, 2304, 3224, 3818, 74, 4647, 401, 12399, 22633, 6877, 388, 299, 8258, 5480, 4724, 25708, 13063, 15844, 2423, 39184, 2333, 4812, 1594, 2423, 39184, 4795, 892, 1500, 337, 24674, 8258, 19273, 15435, 87, 5088], "avg_logprob": -0.3073814644895751, "compression_ratio": 1.4078947368421053, "no_speech_prob": 2.086162567138672e-06, "words": [{"start": 839.5, "end": 839.82, "word": "هنا", "probability": 0.92333984375}, {"start": 839.82, "end": 840.18, "word": " فحص", "probability": 0.9676106770833334}, {"start": 840.18, "end": 840.72, "word": " اسمه", "probability": 0.9734700520833334}, {"start": 840.72, "end": 841.94, "word": " hopkincol", "probability": 0.5911407470703125}, {"start": 841.94, "end": 842.52, "word": " أو", "probability": 0.63623046875}, {"start": 842.52, "end": 843.56, "word": " glycosilic", "probability": 0.8839111328125}, {"start": 843.56, "end": 843.96, "word": " acid", "probability": 0.9501953125}, {"start": 843.96, "end": 844.6, "word": " reaction", "probability": 0.89501953125}, {"start": 844.6, "end": 844.76, "word": " اللي", "probability": 0.6197509765625}, {"start": 844.76, "end": 844.92, "word": " هو", "probability": 0.5263671875}, {"start": 844.92, "end": 845.24, "word": " اسمه", "probability": 0.8193359375}, {"start": 845.24, "end": 846.58, "word": " hopkincol،", "probability": 0.757958984375}, {"start": 846.58, "end": 847.52, "word": " glycosilic", "probability": 0.937744140625}, {"start": 847.52, "end": 847.8, "word": " acid", "probability": 0.966796875}, {"start": 847.8, "end": 848.52, "word": " reaction", "probability": 0.9130859375}, {"start": 848.52, "end": 849.14, "word": " بيدور", "probability": 0.712890625}, {"start": 849.14, "end": 849.4, "word": " على", "probability": 0.90234375}, {"start": 849.4, "end": 849.58, "word": " الـ", "probability": 0.5887451171875}, {"start": 849.58, "end": 850.06, "word": " Indole", "probability": 0.56689453125}, {"start": 850.06, "end": 850.62, "word": " group", "probability": 0.62890625}, {"start": 850.62, "end": 851.42, "word": " الـ", "probability": 0.72802734375}, {"start": 851.42, "end": 851.86, "word": " melons", "probability": 0.517578125}, {"start": 851.86, "end": 852.36, "word": " test", "probability": 0.83544921875}, {"start": 852.36, "end": 852.94, "word": " for", "probability": 0.90380859375}, {"start": 852.94, "end": 853.4, "word": " amino", "probability": 0.90869140625}, {"start": 853.4, "end": 853.92, "word": " acid", "probability": 0.8818359375}, {"start": 853.92, "end": 854.76, "word": " containing", "probability": 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0.9658203125}, {"start": 966.7, "end": 966.84, "word": " على", "probability": 0.94970703125}, {"start": 966.84, "end": 967.26, "word": " نظام", "probability": 0.9931640625}, {"start": 967.26, "end": 967.84, "word": " معادلات", "probability": 0.949951171875}, {"start": 967.84, "end": 968.32, "word": " بيسهل", "probability": 0.84111328125}, {"start": 968.32, "end": 968.52, "word": " عليه", "probability": 0.93115234375}, {"start": 968.52, "end": 969.52, "word": " كيف", "probability": 0.91845703125}, {"start": 969.52, "end": 969.76, "word": " هذا", "probability": 0.93603515625}, {"start": 969.76, "end": 970.14, "word": " الفحص", "probability": 0.8367513020833334}, {"start": 970.14, "end": 970.3, "word": " له", "probability": 0.38671875}, {"start": 970.3, "end": 970.46, "word": " ال", "probability": 0.9697265625}, {"start": 970.46, "end": 971.36, "word": " principle؟", "probability": 0.91162109375}, {"start": 971.36, "end": 971.66, "word": " إنه", "probability": 0.701171875}, {"start": 971.66, "end": 972.02, "word": " عندنا", "probability": 0.951904296875}, {"start": 972.02, "end": 972.38, "word": " amino", "probability": 0.8232421875}, {"start": 972.38, "end": 972.66, "word": " acid", "probability": 0.947265625}, {"start": 972.66, "end": 972.86, "word": " أنا", "probability": 0.80322265625}, {"start": 972.86, "end": 973.14, "word": " بضيف", "probability": 0.9493815104166666}, {"start": 973.14, "end": 973.46, "word": " حمض", "probability": 0.9300130208333334}, {"start": 973.46, "end": 974.22, "word": " أميني،", "probability": 0.75107421875}, {"start": 974.22, "end": 974.54, "word": " بضيف", "probability": 0.9386393229166666}, {"start": 974.54, "end": 974.82, "word": " عليه", "probability": 0.88818359375}, {"start": 974.82, "end": 975.94, "word": " نينهيدرينرياشا", "probability": 0.5678304036458334}, {"start": 975.94, "end": 976.68, "word": " إيش", "probability": 0.7102864583333334}, {"start": 976.68, "end": 977.1, "word": " بيعمل", "probability": 0.876953125}, {"start": 977.1, "end": 977.24, "word": " ال", "probability": 0.55322265625}], "temperature": 1.0}], "language": "ar", "language_probability": 1.0, "duration": 977.351125, "duration_after_vad": 945.0918750000003}
\ No newline at end of file
diff --git a/PL9fwy3NUQKwZ1Q7zOg1ObgufPMjRI3WY1/106ueYAKuA4_raw.srt b/PL9fwy3NUQKwZ1Q7zOg1ObgufPMjRI3WY1/106ueYAKuA4_raw.srt
new file mode 100644
index 0000000000000000000000000000000000000000..a838de44d0dc755cc2f21f96f7add1b1fa5ed0e3
--- /dev/null
+++ b/PL9fwy3NUQKwZ1Q7zOg1ObgufPMjRI3WY1/106ueYAKuA4_raw.srt
@@ -0,0 +1,864 @@
+1
+00:00:00,180 --> 00:00:04,740
+الان شاء الله بدنا نحكي فيه الفحوصات المستخدمة
+
+2
+00:00:04,740 --> 00:00:09,720
+للكشف عن الأحماض الأمينية qualitative test of
+
+3
+00:00:09,720 --> 00:00:15,040
+amino acid بدنا نحكي عن الفحوصات النوعية المستخدمة
+
+4
+00:00:15,040 --> 00:00:19,640
+للكشف عن الأحماض الأمينية qualitative تعني نوعية
+
+5
+00:00:20,300 --> 00:00:24,480
+يعني هذه الفحوصات أو النتيجة اللي هتظهر عندي هتكون
+
+6
+00:00:24,480 --> 00:00:29,440
+عبارة عن positive أو negative يوجد أو لا يوجد
+
+7
+00:00:29,440 --> 00:00:35,940
+qualitative معناها فحوصات نوعية أو جودة تعطي نتيجة
+
+8
+00:00:35,940 --> 00:00:41,640
+positive أو negative ظهور لون أو عدم ظهور لون، لا
+
+9
+00:00:41,640 --> 00:00:45,140
+تعطي رقم في عندنا نوعتان من الفحوصات اللي هو
+
+10
+00:00:45,140 --> 00:00:51,030
+quantitative testQuantitative معناها كمية تحليل كم
+
+11
+00:00:51,030 --> 00:00:55,670
+يعني بديطلع عندك كمية أو مقدار يعني هيطلع عندك في
+
+12
+00:00:55,670 --> 00:00:59,850
+الآخر عدد تركيز بس اللي احنا هنتعرف عليه اليوم هو
+
+13
+00:00:59,850 --> 00:01:06,210
+qualitative test for amino acid فحوصات نوعية للكشف
+
+14
+00:01:06,210 --> 00:01:12,950
+عن الأحمض الأمينيةفي البداية نحكي مقدمة عن الأحماض
+
+15
+00:01:12,950 --> 00:01:17,130
+الأمينية و هذه الأشياء حكيناها في المعمل الماضي
+
+16
+00:01:17,130 --> 00:01:21,850
+حكينا إن الطعام ينقسم إلى تلاتة إلى carbohydrate و
+
+17
+00:01:21,850 --> 00:01:25,270
+lipid و protein حكينا ال carbohydrate هو مصدر
+
+18
+00:01:25,270 --> 00:01:29,470
+الطاقة ال lipid هو مخزن الطاقة و ال protein هو
+
+19
+00:01:29,470 --> 00:01:34,250
+طاقة الخلايا لبناء الخلايا و للتجد حكيناها المرة
+
+20
+00:01:34,250 --> 00:01:39,900
+الماضيةبرضه حكينا عن الأحماض الأمينية إنه هي عبارة
+
+21
+00:01:39,900 --> 00:01:44,180
+عن الوحدة البنائية للبروتين وحكينا إنها بترتبط مع
+
+22
+00:01:44,180 --> 00:01:47,680
+بعض بـprotein bond حكينا الصيغة العامة للأحماض
+
+23
+00:01:47,680 --> 00:01:51,240
+الأمينية عبارة عن H وإن كل حمض أمين بيختلف عن
+
+24
+00:01:51,240 --> 00:01:57,580
+الآخر بال R ال side ال chain حكينا كمان المرة
+
+25
+00:01:57,580 --> 00:01:59,780
+الماضية إن الأحماض الأمينية
+
+26
+00:02:03,760 --> 00:02:06,880
+وين بتيجي الرابط اللي بيبتديها؟ بين مجموع كربوكسيل
+
+27
+00:02:06,880 --> 00:02:11,700
+من حمض أميني ومجموع أمين من حمض أميني آخر يتم نزع
+
+28
+00:02:11,700 --> 00:02:15,960
+جزء ماء في هذه العملية فسمينا التفاعلات تكون
+
+29
+00:02:15,960 --> 00:02:20,800
+الرابط اللي بيبتديها بـ Condensation Reaction لأنه
+
+30
+00:02:20,800 --> 00:02:24,500
+بينتج عنها ماء حكينا على الأطراف بيظل عند Free
+
+31
+00:02:24,500 --> 00:02:28,920
+Amino Group بنسميها الـ N Terminus و Free Carboxyl
+
+32
+00:02:28,920 --> 00:02:35,040
+Group سميناها الـ C Terminusهنا كمان إن الأحماض
+
+33
+00:02:35,040 --> 00:02:39,000
+الأمنية تنقسم حسب المصدر إلى essential و non
+
+34
+00:02:39,000 --> 00:02:43,320
+-essential ال essential الجسم غير قادر على تصنيعها
+
+35
+00:02:43,320 --> 00:02:47,040
+يجب توفيرها في الواجبات ال non-essential الجسم
+
+36
+00:02:47,040 --> 00:02:51,420
+قادر على تصنيحها فمش ضرور تكون موجودة في الواجبات
+
+37
+00:02:51,420 --> 00:02:58,610
+اليوميةو هنا حكينا أن أبسط حمض أميني هو ال glycine
+
+38
+00:02:58,610 --> 00:03:02,590
+ليش؟ لأن ال side-chain أو ال R-group هي عبارة عن
+
+39
+00:03:02,590 --> 00:03:06,730
+طرد هيدروجين فقط و حكينا كمان أن الحمض الأمين
+
+40
+00:03:06,730 --> 00:03:10,990
+الشاز أو الفريد باختلف عن غيره من الأحماض الأمينية
+
+41
+00:03:10,990 --> 00:03:16,430
+هو البرولينلأنه ماعندهوش amino group حرة اللي
+
+42
+00:03:16,430 --> 00:03:19,890
+متصلة في الـ central carbon سمناها primary amino
+
+43
+00:03:19,890 --> 00:03:24,530
+group في كل الأحماض الأمينية تسمى مجموعة الـ amino
+
+44
+00:03:24,530 --> 00:03:30,030
+group هي primary amino group أو free alpha amino
+
+45
+00:03:30,030 --> 00:03:35,030
+group إلا في حالة البرولين تسمى secondary amino
+
+46
+00:03:35,030 --> 00:03:39,270
+group أو amino group لأنها متصلة بالـ central
+
+47
+00:03:39,270 --> 00:03:47,470
+carbon من جهة بالـ sided chainمن جهة أخرى حكينا عن
+
+48
+00:03:47,470 --> 00:03:51,670
+الأحماض الأمنية وتقسيمها حسب ال polarity إلى polar
+
+49
+00:03:51,670 --> 00:03:53,050
+و non polar
+
+50
+00:03:56,470 --> 00:04:00,650
+charge و ال charge في منها positive charge و في
+
+51
+00:04:00,650 --> 00:04:04,790
+منها negative charge فهذه التقسيمة كلها سواء polar
+
+52
+00:04:04,790 --> 00:04:09,510
+او non polar تعتمد على ال side chain بتطلع على ال
+
+53
+00:04:09,510 --> 00:04:13,430
+side chain حكينا حكي ان اذا في بس كربون و هيدروجين
+
+54
+00:04:13,430 --> 00:04:18,930
+بتكون non polar اذا فيها مجموعات قطبية بتكون polar
+
+55
+00:04:18,930 --> 00:04:22,670
+بتطلع اذا كانت مشحونة بتكون charge و اذا غير
+
+56
+00:04:22,670 --> 00:04:25,390
+مشحونة بتكون uncharged
+
+57
+00:04:27,110 --> 00:04:31,130
+حكينا عن هذه الصورة اللي بتقسم للأحماض الأمينية
+
+58
+00:04:31,130 --> 00:04:35,610
+إلى non-polar و polar ال polar في منها uncharged و
+
+59
+00:04:35,610 --> 00:04:39,950
+في منها charged و في positive و في negative حكينا
+
+60
+00:04:39,950 --> 00:04:43,510
+في أحماض أمينية بنسميها aromatic amino acid
+
+61
+00:04:43,510 --> 00:04:47,070
+aromatic اللي هي بتحتوي في ال side chain على حلقة
+
+62
+00:04:47,070 --> 00:04:50,650
+بنزين إذا كان في ال side chain في حلقة بنسميها
+
+63
+00:04:50,650 --> 00:04:53,290
+aromatic amino acid
+
+64
+00:04:56,910 --> 00:05:00,770
+ويجي نحكي عن حاجة اسمها Ionization Ionization
+
+65
+00:05:00,770 --> 00:05:05,110
+معناها كيف يتصرف الحمض الأميني في الأوساط
+
+66
+00:05:05,110 --> 00:05:10,190
+المختلفة، في وسط قاعدي، في وسط حمضي، في neutral،
+
+67
+00:05:10,190 --> 00:05:15,330
+في اتش، في وسط المتعانيهلجت لو جينا و اتفرج على
+
+68
+00:05:15,330 --> 00:05:19,210
+المعادلة هاي اللي فوق في الوسط هذا حمض أميني موجود
+
+69
+00:05:19,210 --> 00:05:24,350
+في وسط متعدد الصيغة العامة لحمض أميني موجود في وسط
+
+70
+00:05:24,350 --> 00:05:29,790
+متعدد بتكون عدد الشحنات الموجبة بتساوي عدد الشحنات
+
+71
+00:05:29,790 --> 00:05:33,690
+السالمة ان هنا مكتوب CH2 هو الصيغة العامة للحمض
+
+72
+00:05:33,690 --> 00:05:38,150
+الأميني Central Carbon و Hydrogen و ال R group هي
+
+73
+00:05:38,150 --> 00:05:41,250
+عبارة عن ذرة الهيدروجين المعتبرينها هناوانقدش
+
+74
+00:05:41,250 --> 00:05:45,670
+three plus و COO minus هي الصيغة العامة للحمد
+
+75
+00:05:45,670 --> 00:05:49,090
+الأميني في الوسط المتعادل تكون الشحنات الموجبة
+
+76
+00:05:49,090 --> 00:05:54,830
+بتساوي الشحنات السالمةالجهاد الـ net charge بتكون
+
+77
+00:05:54,830 --> 00:05:59,330
+زيه لو جيت أخدت هذا الحمض الأميني حاطيته في وسط
+
+78
+00:05:59,330 --> 00:06:07,010
+حمضي وسط حمضي يعني جلالة الـ pH يعني أنه كان فيه
+
+79
+00:06:07,010 --> 00:06:11,130
+زيادة في أيونات الهيدروجين يعني هذا الوسط مليء بـ
+
+80
+00:06:11,130 --> 00:06:16,620
+H plus زيادةفالحمض الأمينى دخلته من المتعدل وحطيته
+
+81
+00:06:16,620 --> 00:06:20,120
+في وسط الحمضي وسط الحمضي فيه زيادة في أيونات
+
+82
+00:06:20,120 --> 00:06:24,400
+الهيدروجين الجاتمين بده يحتاج الهيدروجين هياخد من
+
+83
+00:06:24,400 --> 00:06:28,920
+الوسط COO- محتاج الهيدروجين فبتستحب من الوسط
+
+84
+00:06:28,920 --> 00:06:37,990
+هيدروجين و بتتشبع ب CRCOOHفبتصير CH2 COOH NH3 Plus
+
+85
+00:06:37,990 --> 00:06:44,990
+بصير الشكل العام له CH2 COOH NH3 Plus فأخذ ذرة
+
+86
+00:06:44,990 --> 00:06:49,960
+هيدروجيني صارت النت تشارج عليه positive chargeاللي
+
+87
+00:06:49,960 --> 00:06:53,040
+موجودة في المعادلة على اليسار هذا اللي هو الصيغة
+
+88
+00:06:53,040 --> 00:06:59,480
+العامة الحمض الأميني في وسط حمضي CH2COHNH3Plus
+
+89
+00:06:59,480 --> 00:07:03,580
+إنه الكربوكسيل أخدت أيون هيدروجين أو بروتون
+
+90
+00:07:03,580 --> 00:07:08,720
+هيدروجين من الوسط الحمضي اللي هي فيه فصارت COH نت
+
+91
+00:07:08,720 --> 00:07:13,960
+تشارج في الوسط الحمضي صارتPositive charge طبعا لو
+
+92
+00:07:13,960 --> 00:07:18,060
+أخدتها الوسط من الوسط المتعدد وحطته في وسط قاعدي
+
+93
+00:07:18,060 --> 00:07:25,280
+الوسط القاعدي مليء بـ OH- وسط بيكون مليء بـ OH- OH
+
+94
+00:07:25,280 --> 00:07:30,140
+- هي تحتاج إلى ذرة هيدروجين عشان تتكون جزئنا
+
+95
+00:07:30,140 --> 00:07:35,920
+فبتروح على أي ذر جزية بتنزع منه هيدروجين عشان
+
+96
+00:07:35,920 --> 00:07:40,320
+تتكوننا من عندنا بنقدر ننزع منه هيدروجين لأن اتش 3
+
+97
+00:07:40,320 --> 00:07:46,960
+plusتاخد منه هيدروجين فبصير NH2 COO minus هتتغير؟
+
+98
+00:07:46,960 --> 00:07:52,300
+لأ مش هتتغير فالوسط المتعدل CH2 NH3 plus COO minus
+
+99
+00:07:52,300 --> 00:07:57,360
+لما يروح على القاعدة اللي هي على اليمين بيصير NH2
+
+100
+00:07:57,360 --> 00:08:02,860
+و COO minus اتغيرت من الـ NH3 نزعنا منها هيدروجين
+
+101
+00:08:02,860 --> 00:08:07,600
+و صارت NH2الـ net charge إيه لو بيكون minus ففي
+
+102
+00:08:07,600 --> 00:08:11,920
+حالة الوسط الحمضي .. في حالة وسط متعادل ال net
+
+103
+00:08:11,920 --> 00:08:16,360
+charge بتكون zero فحالة وسط حمضي بيكون ال net
+
+104
+00:08:16,360 --> 00:08:20,100
+charge positive في حالة الوسط القاعدي ال net
+
+105
+00:08:20,100 --> 00:08:24,700
+charge negativeهلجد؟ الحمض الأميني اللي بتكون
+
+106
+00:08:24,700 --> 00:08:29,120
+متساوي عليه الشحنات الموجبة و الشحنات السالبة و ال
+
+107
+00:08:29,120 --> 00:08:32,960
+net charge بتكون بتساوي zero بسميه Zwitterion
+
+108
+00:08:32,960 --> 00:08:37,580
+Zwitterion هو الحمض الأميني اللي تتساوي عليه
+
+109
+00:08:37,580 --> 00:08:42,180
+الشحنة الموجبة و الشحنة السالبة يعني الشحنة الكلية
+
+110
+00:08:42,180 --> 00:08:46,280
+تكون صفر وين موجود ال Zwitterion؟ في الوسط
+
+111
+00:08:46,280 --> 00:08:50,650
+المتعادل كان موجودبالوسط المتعدل الحمض الأميني
+
+112
+00:08:50,650 --> 00:08:54,790
+بيكون الشحن الموجبة بتساوي الشحن السالي يعني الشحن
+
+113
+00:08:54,790 --> 00:08:59,950
+الكلية بتساوي سفر وهذا بسميه Zwitterion Zwitterion
+
+114
+00:08:59,950 --> 00:09:03,870
+هو الحمض الأميني الذي تتساوي عليه الشحن الموجبة
+
+115
+00:09:03,870 --> 00:09:10,520
+والشحن السالي وندت شهر Zeroعند الوسط المتعادل هذا،
+
+116
+00:09:10,520 --> 00:09:13,240
+عند تكون الحمض الأميني اللي بتكون شحنات الموجبة
+
+117
+00:09:13,240 --> 00:09:16,340
+السالبة بتساوي الموجبة، في حاجة بنتسميها
+
+118
+00:09:16,340 --> 00:09:21,100
+isoelectric point أو الـ PR الـ PI أو الـ
+
+119
+00:09:21,100 --> 00:09:26,540
+isoelectric point هي قيمة BH بتتساوي عند الشحن
+
+120
+00:09:26,540 --> 00:09:32,320
+الموجبة والسالبة على سطح الحمض الأمينيوبالتالي،
+
+121
+00:09:32,320 --> 00:09:38,620
+شحنة الكلية صفر قيمة BH تتساوى عندها شحنة الموجبة
+
+122
+00:09:38,620 --> 00:09:43,760
+والسالبة على سطح الحمض الأمين وبالتالي، شحنة
+
+123
+00:09:43,760 --> 00:09:47,640
+الكلية بتساوي صفر هاي الـ Isoelectric point،
+
+124
+00:09:47,640 --> 00:09:53,260
+الإفتصار بـI تمام، في حاجة بنسميها protonated
+
+125
+00:09:53,260 --> 00:09:57,740
+معناها إنه اكتساب بروتون أو H plus في حاجة بنسميها
+
+126
+00:09:57,740 --> 00:10:02,780
+Deprotonated يعني فقد بروتوناللي بياخد H plus يعني
+
+127
+00:10:02,780 --> 00:10:07,800
+في الوسط الحمض زي COO minus اكتسبت H فهذه gain of
+
+128
+00:10:07,800 --> 00:10:12,800
+البروتون يعني صارلها protonated Deprotonated يعني
+
+129
+00:10:12,800 --> 00:10:16,760
+loss of البروتون زي الـ NH3 plus في الوسط القاعدة
+
+130
+00:10:16,760 --> 00:10:22,060
+لما حضيتها صارت NH2 نزعت منها H فبسميها .. بقول
+
+131
+00:10:22,060 --> 00:10:26,600
+عنها نصارلها Deprotonated فقدت البروتون
+
+132
+00:10:30,550 --> 00:10:34,490
+هنا بيحكيلي أن الـ Isoelectric point أو الـ PI هي
+
+133
+00:10:34,490 --> 00:10:39,090
+قيمة BH اللي بيكون فيها الانيونك بتساوي الكاتيونك
+
+134
+00:10:39,090 --> 00:10:45,350
+النت تشارج بتكون Zero كل حمض أميني له PI أو
+
+135
+00:10:45,350 --> 00:10:49,490
+Isoelectric point بتختلف عن غيره حسب شو؟ حسب ال
+
+136
+00:10:49,490 --> 00:10:52,830
+side chain فهو حاكي أن كل حمض أميني بيميزه عن غيره
+
+137
+00:10:52,830 --> 00:10:54,110
+side chain
+
+138
+00:10:56,690 --> 00:11:01,790
+نحكي في الجزء العملي للمعمل الأول جزء العملي بيحكي
+
+139
+00:11:01,790 --> 00:11:06,930
+عن qualitative test for amino acid بحوصات جودة
+
+140
+00:11:06,930 --> 00:11:10,710
+لالكشف عن الأحماض الأمينية النتيجة عندنا هتكون
+
+141
+00:11:10,710 --> 00:11:16,330
+positive أو negative«ظهور لون» أو «عدم ظهور لون»
+
+142
+00:11:16,330 --> 00:11:19,450
+في عندنا number of tests to detect the presence of
+
+143
+00:11:19,450 --> 00:11:23,210
+amino acid هذه الفحوصات الكاشف عن الأحمان الأمينية
+
+144
+00:11:23,210 --> 00:11:26,830
+largely depend on the nature of sided chain علشان
+
+145
+00:11:26,830 --> 00:11:29,770
+بيعتمدوا على الـsided chain لأنه حكي أن البيميز
+
+146
+00:11:29,770 --> 00:11:34,090
+حمض أمين عن الآخر هو الـsided chain الجد احنا
+
+147
+00:11:34,090 --> 00:11:38,210
+الفحوصات اللي هنشتغلها بيطلع معنا لون يتغير أو
+
+148
+00:11:38,210 --> 00:11:44,060
+مايتغيرهذا اللون له شدة intensity of color شدة
+
+149
+00:11:44,060 --> 00:11:47,940
+اللون هي بتكون تختلف يعني ممكن هو يتلون بس على
+
+150
+00:11:47,940 --> 00:11:52,580
+درجات من حاجة لتانية تكون درجة بتختلف شدة اللون هي
+
+151
+00:11:52,580 --> 00:11:56,860
+علشان بتعتمد بتعتمد على أول شي number of reacting
+
+152
+00:11:56,860 --> 00:12:00,900
+unit number of reacting unit اللي هي ال reacting
+
+153
+00:12:00,900 --> 00:12:05,040
+unit هي عبارة عن المادة الفعالةقداش عدد المادة
+
+154
+00:12:05,040 --> 00:12:08,780
+الفعالة الموجودة؟ إيش هي المادة الفعالة؟ دي الاشي
+
+155
+00:12:08,780 --> 00:12:12,940
+اللي بدور عليها الفحص أنا بشتغل فحص مثلا بيدور على
+
+156
+00:12:12,940 --> 00:12:15,980
+peptide bond إذا موجودة ال peptide bond، يعطيني
+
+157
+00:12:15,980 --> 00:12:19,900
+لون إذا مش موجودة، مش هيتغير اللون طب ال peptide
+
+158
+00:12:19,900 --> 00:12:23,160
+bond هذي ممكن تكون بس رابطتين، ويعطيني لون ممكن
+
+159
+00:12:23,160 --> 00:12:26,400
+تكون تلاتة ويعطيني لون بس هل اللون هيكون نفسه إذا
+
+160
+00:12:26,400 --> 00:12:30,080
+كانوا تنتين وإذا كانوا تلاتة؟ شدة اللون هتختلف
+
+161
+00:12:30,080 --> 00:12:34,810
+بناء على number of reacting unitالحاجة التانية
+
+162
+00:12:34,810 --> 00:12:37,890
+اللي بتعتمد عليها شدة اللون هي الـ accessibility
+
+163
+00:12:37,890 --> 00:12:42,050
+to reaction هل هذه المادة الفعالة الـ acting unit
+
+164
+00:12:42,050 --> 00:12:46,350
+قريبة من التفاعل، هل هي حرة، free، بتقدر تيجي
+
+165
+00:12:46,350 --> 00:12:48,990
+ترتبط، تشتغل مع التفاعل، مع الـ agent اللي أنا
+
+166
+00:12:48,990 --> 00:12:52,910
+حطيته، تشتغل و تتغير اللون، ولا إنها مشغولة، مش
+
+167
+00:12:52,910 --> 00:12:56,930
+هتقدر في حواجز، مش هتقدر تيجي ترتبطهي بتسميها
+
+168
+00:12:56,930 --> 00:13:00,810
+accessibility to reaction إذا تشدت اللون تعتمد على
+
+169
+00:13:00,810 --> 00:13:04,450
+حاشتين على ال number of reacting unit وعلى
+
+170
+00:13:04,450 --> 00:13:10,470
+accessibility to reaction إيش
+
+171
+00:13:10,470 --> 00:13:13,570
+هي الفحصات اللي هنستخدمها للكشف عن الأحماض
+
+172
+00:13:13,570 --> 00:13:19,070
+الأمينية؟ هنا فحص اسمه ninhydrin test for 3-alpha
+
+173
+00:13:19,070 --> 00:13:24,160
+amino groupعلى Free Alpha Amino Group إذا موجودة
+
+174
+00:13:24,160 --> 00:13:27,280
+بيتغير اللون وبيعطى positive إذا مش موجودة مش
+
+175
+00:13:27,280 --> 00:13:30,880
+هيتغير اللون فالـ Free Alpha Amino Group هاي
+
+176
+00:13:30,880 --> 00:13:35,000
+بسميها الـ Reacting Unit فحص التاني اسمه Bio-Red
+
+177
+00:13:35,000 --> 00:13:39,240
+Test بتدور على ال-peptide bond لإنه فحص ال
+
+178
+00:13:39,240 --> 00:13:45,040
+-xanthoproteic testZanto Proteic Test for Aromatic
+
+179
+00:13:45,040 --> 00:13:51,240
+Amino Acid لأ
+
+180
+00:13:51,240 --> 00:13:55,300
+الأحماض الأمنية اللي تحتوي على حلقة بنزين نشطة
+
+181
+00:13:55,300 --> 00:14:00,720
+نضيف هنا Activated Aromatic Amino Acidهنا فحص اسمه
+
+182
+00:14:00,720 --> 00:14:05,240
+hopkincol أو glycosilic acid reaction اللي هو اسمه
+
+183
+00:14:05,240 --> 00:14:09,580
+hopkincol، glycosilic acid reaction بيدور على الـ
+
+184
+00:14:09,580 --> 00:14:13,920
+Indole group الـ melons test for amino acid
+
+185
+00:14:13,920 --> 00:14:18,800
+containing hydroxylفينال جروب للفينول جروب الأمينو
+
+186
+00:14:18,800 --> 00:14:23,100
+أسد اللي بيحتوي على فينول جروب ساكيجوشي test for
+
+187
+00:14:23,100 --> 00:14:28,160
+ليجوانيديام جروب لليجوانيديام جروب ليد سالفايت
+
+188
+00:14:28,160 --> 00:14:33,080
+test بيدور على ال self-hydraile group للحمض الأمين
+
+189
+00:14:33,080 --> 00:14:36,240
+اللي يحتوي على self-hydraile group هو اللي هيعطيني
+
+190
+00:14:36,240 --> 00:14:39,360
+positive غير هيك مش هيعطيني positive
+
+191
+00:14:42,170 --> 00:14:46,290
+نجي نحكي عن أول فحص، أول فحص هو الـ Nenhedrine الـ
+
+192
+00:14:46,290 --> 00:14:49,010
+Nenhedrine إيش ال objective للفحص، إيش الهدف من
+
+193
+00:14:49,010 --> 00:14:52,730
+الفحص؟ to detect the presence of free alpha amino
+
+194
+00:14:52,730 --> 00:14:56,950
+group، بيدور على الـ free alpha amino group إذا
+
+195
+00:14:56,950 --> 00:15:00,970
+موجود free alpha amino group، هيتغير اللون،
+
+196
+00:15:00,970 --> 00:15:04,330
+بيعطيني لون بارد، في حالات اللي موجودة في free
+
+197
+00:15:04,330 --> 00:15:07,930
+alpha amino groupمين عنده free alpha amino group؟
+
+198
+00:15:07,930 --> 00:15:11,390
+إذا ذاكرين الحمض الأميني، أيش الصيغة العامة له؟
+
+199
+00:15:11,390 --> 00:15:14,750
+تكون من center carbon ومن الهيدروجينة ومن ال side
+
+200
+00:15:14,750 --> 00:15:18,530
+chain ومن carboxyl group ومن amino group amino
+
+201
+00:15:18,530 --> 00:15:23,350
+group هاي سميناها primary amino group أو alpha
+
+202
+00:15:23,350 --> 00:15:27,530
+amino group معادة في حالة البرولين سميناها amino
+
+203
+00:15:27,530 --> 00:15:32,840
+groupيعني الأحماض الأمينية كلها تحتوي على free
+
+204
+00:15:32,840 --> 00:15:37,700
+alpha amino group ما عدا لبرولين عنده amino group
+
+205
+00:15:37,700 --> 00:15:40,760
+يعني مين هيعطيني positive مع هذا الفحص لو أنا
+
+206
+00:15:40,760 --> 00:15:44,440
+اشتغلت مجموعة أحماض أمينية على هذا الفحص؟ كلهم
+
+207
+00:15:44,440 --> 00:15:47,540
+هيعطوني positive لأنهم كلهم بيحتوي على free alpha
+
+208
+00:15:47,540 --> 00:15:53,040
+amino group تمام؟طيب، إذا ال objective to detect
+
+209
+00:15:53,040 --> 00:15:55,520
+the presence of free alpha amino group هل موجود
+
+210
+00:15:55,520 --> 00:15:58,420
+free alpha amino group أو لا؟ إذا تغير اللغة يعني
+
+211
+00:15:58,420 --> 00:16:01,560
+موجود، إذا ما تغيرش يعني مش موجود طيب إيش ال
+
+212
+00:16:01,560 --> 00:16:05,620
+principle؟ مبتدأ العمل للفحص؟ كيف يشتغل الفحص؟ ال
+
+213
+00:16:05,620 --> 00:16:08,520
+principle لو نعملها على نظام معادلات بيسهل عليه
+
+214
+00:16:08,520 --> 00:16:12,380
+كيف هذا الفحص له ال principle؟ إنه عندنا amino
+
+215
+00:16:12,380 --> 00:16:15,940
+acid أنا بضيف حمض أميني، بضيف عليه نينهيدرينرياشا
+
+216
+00:16:15,940 --> 00:16:17,240
+إيش بيعمل ال
+
diff --git a/PL9fwy3NUQKwZ1Q7zOg1ObgufPMjRI3WY1/42Zoe_ECvac.srt b/PL9fwy3NUQKwZ1Q7zOg1ObgufPMjRI3WY1/42Zoe_ECvac.srt
new file mode 100644
index 0000000000000000000000000000000000000000..ad21a9448882137837f7535c793a386794e339dd
--- /dev/null
+++ b/PL9fwy3NUQKwZ1Q7zOg1ObgufPMjRI3WY1/42Zoe_ECvac.srt
@@ -0,0 +1,682 @@
+1
+00:00:00,000 --> 00:00:03,480
+هنبدأ  الجزء بالجزء العامل الـ Experiment اللي هي
+
+2
+00:00:03,480 --> 00:00:08,610
+التجربة،أولا نحكي عن بعض المصطلحات الـ illusion
+
+3
+00:00:08,610 --> 00:00:12,250
+تحكينا هو عبارة عن الـ mobile phase تحكينا الـ
+
+4
+00:00:12,250 --> 00:00:16,050
+mobile phase ممكن أسميه solvent ممكن أسميه liquid
+
+5
+00:00:16,050 --> 00:00:20,430
+ممكن أسميه illusion، الـ illusion هي motion of
+
+6
+00:00:20,430 --> 00:00:23,730
+mobile phase through the stationary phase هي حركة
+
+7
+00:00:23,730 --> 00:00:27,250
+الـ mobile phase عبر الـ stationary phase لما يمشي
+
+8
+00:00:27,250 --> 00:00:30,470
+السائل اللي هو الـ mobile phase من الورقة اللي هي
+
+9
+00:00:30,470 --> 00:00:34,400
+الـ stationary phase هذه الحركة بسميها illusion، الـ
+
+10
+00:00:34,400 --> 00:00:40,480
+illusion time هو الوقت 
+
+11
+00:00:40,480 --> 00:00:44,700
+اللذي يحتاجه الـ mobile phase للمرور عبر
+
+12
+00:00:44,700 --> 00:00:50,060
+الستشيراري phase اللي بيكون له شهر illusion time
+
+13
+00:00:50,450 --> 00:00:54,270
+فهو بيمر أسرع عبر الـ stationary phase اللي بيكون
+
+14
+00:00:54,270 --> 00:00:59,150
+وقته أقل، أخد واجهة أجال، إذا هو ماشي أسرع، الـ
+
+15
+00:00:59,150 --> 00:01:01,890
+illusion time هو الوقت اللي احتاجه الـ mobile phase
+
+16
+00:01:01,890 --> 00:01:05,710
+عشان يمشي عبر الـ stationary phase، الـ mobile phase
+
+17
+00:01:05,710 --> 00:01:09,390
+هو part of a chromatography system that is mobile
+
+18
+00:01:09,390 --> 00:01:13,290
+commonly a solvent mixture زي اللي في الـ column
+
+19
+00:01:13,290 --> 00:01:16,730
+chromatography أو thin layer chromatography or gas
+
+20
+00:01:16,730 --> 00:01:21,030
+as gas in gas chromatography، الـ mobile phase هو جزء
+
+21
+00:01:21,030 --> 00:01:23,970
+من أجزاء الـ chromatographic system هما جزءين الـ
+
+22
+00:01:23,970 --> 00:01:27,130
+stationary و الـ mobile، الـ mobile هو أحد أجزاءه
+
+23
+00:01:27,130 --> 00:01:30,290
+بيكون عبارة عن solvent عبارة أو ممكن يكون liquid
+
+24
+00:01:30,290 --> 00:01:36,390
+أو gas، في تجربتنا لليوم، إيش الأدوات اللي بدي
+
+25
+00:01:36,390 --> 00:01:39,650
+استخدمها؟ بدي استخدم اللي you want أكيد بدي mobile
+
+26
+00:01:39,650 --> 00:01:43,230
+phase، الـ mobile phase اللي هو اللي you want اللي
+
+27
+00:01:43,230 --> 00:01:47,930
+بنستخدمه اليوم هو عبارة عن n-butanol مع acetic
+
+28
+00:01:47,930 --> 00:01:52,850
+acid مع distilled water نسبة خمسة لواحد لخمسة، بحط
+
+29
+00:01:52,850 --> 00:01:56,110
+خمسة من الـ butanol على واحد من الـ acetic acid على
+
+30
+00:01:56,110 --> 00:02:01,190
+خمسة من الـ distilled water و أحركهم لمدة عشر دقايق
+
+31
+00:02:01,190 --> 00:02:05,430
+و بعدين بخليهم يفصلوا لطبقات، باخد الطبقة اللي فوق
+
+32
+00:02:05,430 --> 00:02:10,250
+و بستخدمها ك اليوان ك mobile phase، بدي developing
+
+33
+00:02:10,250 --> 00:02:14,490
+solution developing solution هي دي عبارة عن مادة
+
+34
+00:02:14,490 --> 00:02:19,510
+كيميائية عشان توضح لون المادة المفصولة، اليوم في
+
+35
+00:02:19,510 --> 00:02:23,570
+تجربتنا بدي اجيب .. بدي نجيب خليط من أحماض أمينية
+
+36
+00:02:23,570 --> 00:02:27,470
+خليط يحتوي على عدة أنواع من الأحماض الأمينية، بدي
+
+37
+00:02:27,470 --> 00:02:31,590
+إياه يفصل لمكوناته، لإن بعد ما التقنية تمشي ويفصل
+
+38
+00:02:31,590 --> 00:02:36,490
+إلى مكوناته بتكون عندي نقاط في كل .. كل مكون بيفصل
+
+39
+00:02:36,490 --> 00:02:41,550
+في مكان، هذه النقاط،بتكون colorless بتكون غير ملونة
+
+40
+00:02:41,550 --> 00:02:45,530
+على الورق اللى أنا استخدمتها للفصل، ما بيظهر اللون
+
+41
+00:02:45,530 --> 00:02:50,350
+فهتكون مش هشوف النقاط وين فصلت، فبدي محلول أو مادة
+
+42
+00:02:50,350 --> 00:02:56,270
+تظهرلي اللون، بتغير اللون اللى عندي يعني تحولها
+
+43
+00:02:56,270 --> 00:03:01,150
+تظهرليها بملون، هتكون بألوان تانية، فبدي استخدم مادة
+
+44
+00:03:01,150 --> 00:03:05,200
+بتحددلي اللون المادة المفصولة، هذه المادة اللي اليوم
+
+45
+00:03:05,200 --> 00:03:08,500
+نستخدمها اسمها Developing Solution هي عبارة عن
+
+46
+00:03:08,500 --> 00:03:12,020
+تلاتة من عشرة جرام من الـ ninhydrin على عشرة مل من
+
+47
+00:03:12,020 --> 00:03:16,420
+الـ butanol على تلاتة مل من الـ acetic acid، المغزاة إنه
+
+48
+00:03:16,420 --> 00:03:20,000
+أنا بدي أستخدم محلول يظهر لي لون المادة المفصولة
+
+49
+00:03:20,000 --> 00:03:23,640
+لأنها كانت عبارة عن colorless مالهاش لون فبعد
+
+50
+00:03:23,640 --> 00:03:27,560
+الفصل مش هعرف وين هي وفصلت وين هي وقفت إلا إذا
+
+51
+00:03:27,560 --> 00:03:33,030
+لونتها،أنا اليوم بضفص الأحماض الأمينية، تمام؟ الأحماض
+
+52
+00:03:33,030 --> 00:03:35,590
+الأمينية، أيش الفحص اللي بيستخدم لكشف عن كل
+
+53
+00:03:35,590 --> 00:03:38,370
+الأحماض الأمينية؟ مع كل الأحماض الأمينية بيعطيني
+
+54
+00:03:38,370 --> 00:03:42,790
+positive، من الفحصات اللي في أول معملة أخدناها كان
+
+55
+00:03:42,790 --> 00:03:46,130
+عبارة عن فحص الـ Ninhydrin، الـ Ninhydrin بيعطيني
+
+56
+00:03:46,130 --> 00:03:49,590
+positive مع الـ Free Alpha Amino Group، حكينا كلهم
+
+57
+00:03:49,590 --> 00:03:54,210
+عندهم هاي وبيتحول اللون لبربه، مع ذالب رولين عنده
+
+58
+00:03:54,210 --> 00:03:57,410
+amino group بس بيعطيني برضه لون يلون، يعني المغزاة
+
+59
+00:03:57,410 --> 00:04:02,220
+هيطلع عندى لون، يعني كلهم هيعطوني لهم فهيتلون و أشوف
+
+60
+00:04:02,220 --> 00:04:06,560
+وين هاي ال spot و أكفه، إيش كمان بتستخدم؟ بتستخدم
+
+61
+00:04:06,560 --> 00:04:09,740
+solution of different amino acid، بد الخليط اللي
+
+62
+00:04:09,740 --> 00:04:15,220
+بيحتوي على عدة مكونات من الأحماض الأمينية، و بدي الـ
+
+63
+00:04:15,220 --> 00:04:18,420
+chromatography paper اللي هي الـ stationary face و
+
+64
+00:04:18,420 --> 00:04:21,460
+بدي الـ lotion chamber، الـ chamber اللي هو عبارة عن
+
+65
+00:04:21,460 --> 00:04:26,800
+زي بيكر، بدي أحط فيه السائل اللي هو عبارة عن الـ الـ
+
+66
+00:04:26,800 --> 00:04:31,680
+mobile face، السائل هدون بدي أحطه بشامبر بيكر glass
+
+67
+00:04:31,680 --> 00:04:36,110
+capillary force booting بدي tube، اللي هي الـ
+
+68
+00:04:36,110 --> 00:04:38,910
+Capillary Tube، القنابيب اللى رفيعة، القنابيب
+
+69
+00:04:38,910 --> 00:04:43,550
+الشهرية، اظن استخدمتها بالمقدمة، هي القنابيب عشان
+
+70
+00:04:43,550 --> 00:04:47,890
+تحطلي ال spot، أخد من العينة من الخليط نقطة صغيرة
+
+71
+00:04:47,890 --> 00:04:53,030
+تحطلي مقدار صغير، الـ drying oven، بدي فرن لتشفيف
+
+72
+00:04:53,030 --> 00:04:57,370
+على درجة حرارة 60 درجة سيليزيا، هذا الفرن بس عشان
+
+73
+00:04:57,370 --> 00:05:02,350
+يجفف ويزيد التفاعل بين المواد و الـ Ninhydrin، بيزود
+
+74
+00:05:02,350 --> 00:05:08,750
+التفاعل المواد مع الـ Ninhydrin، كيف خطوات العمل؟ كيف
+
+75
+00:05:08,750 --> 00:05:12,130
+أنا بدي أشتغل التجربة؟ هلجيت أن أنا أخد أولا الـ
+
+76
+00:05:12,130 --> 00:05:17,270
+stationary fast الورقة، تمام، هجيت أنا بحكي لإنه لما
+
+77
+00:05:17,270 --> 00:05:22,150
+تشتغلي بدك تكوني لابسة gloves و بدك تستخدمي قلم
+
+78
+00:05:22,150 --> 00:05:26,090
+رصاص في الرسم عليها، رسم خط البداية و خط النهاية،
+
+79
+00:05:26,090 --> 00:05:29,880
+بدك تستخدمي قلم رصاص، ليش؟ لأنه جلم الحبر لو
+
+80
+00:05:29,880 --> 00:05:33,260
+استخدمتيه هو عبارة عن أصباغ، عبارة عن ألوان، لما
+
+81
+00:05:33,260 --> 00:05:36,440
+تيجي تفصليه ممكن تفصل الألوان معاك، اللي هو اللي
+
+82
+00:05:36,440 --> 00:05:40,560
+موجودة فيه الجلم الحمر، و بدي بس ألبس gloves عشان
+
+83
+00:05:40,560 --> 00:05:44,600
+أتجنب الـ contamination، ده بعيد عن الـ contamination
+
+84
+00:05:44,600 --> 00:05:48,980
+ليش؟ لأن جلدي الإنسان عبارة عن حماض أمنية في الجلب
+
+85
+00:05:48,980 --> 00:05:52,400
+موجودة، فانت لو استخدمت الورقة بيدك من غير gloves
+
+86
+00:05:53,090 --> 00:05:56,830
+بصمات اسمك اللى بتحتوي على أحماض أمينية بتطبق على
+
+87
+00:05:56,830 --> 00:06:00,190
+الورقة، ممكن تفصل ويصير عندك وانت مانعش ان يصير
+
+88
+00:06:00,190 --> 00:06:04,250
+عندك في أحماض أمينية جاية من الإيد من الجل، مش انها
+
+89
+00:06:04,250 --> 00:06:08,830
+من الخليط تمام؟ الجيتكى بده يشتغل بده يجيب الـ
+
+90
+00:06:08,830 --> 00:06:12,210
+stationary phase اللى عبارة عن ورقة، علنا اليوم
+
+91
+00:06:12,210 --> 00:06:17,560
+stationary phase، الورقة هاي بتكون برس متقبة، تمام،
+
+92
+00:06:17,560 --> 00:06:23,440
+بدي أعمل فيها خط بداية، اللي من عنده بدي أحط الخليط،
+
+93
+00:06:23,440 --> 00:06:28,090
+بدي أحط نقطة من المادة اللي بدي أفصلها، كيف بده أحط
+
+94
+00:06:28,090 --> 00:06:31,710
+خط البداية؟ بيحكيلي بنجيس من تحت من أسفل الورقة
+
+95
+00:06:31,710 --> 00:06:37,430
+لفوق، بنجيس 8 من 10 سانتي، 8 من 10 سانتي من عندها
+
+96
+00:06:37,430 --> 00:06:41,470
+بحط خط البداية، إذا من تحت لفوق بدي أجيس 8 من 10
+
+97
+00:06:41,470 --> 00:06:46,590
+سانتي و أرسم خط البداية، من فوق من الـ end line، من
+
+98
+00:06:46,590 --> 00:06:50,790
+خط النهاية، لوين بده يوصل؟ من فوق بدي أجيس من فوق 5
+
+99
+00:06:50,790 --> 00:06:56,080
+من 10 سانتي، تمام؟ و بعدين إذا عندي أكتر من خليط بدي
+
+100
+00:06:56,080 --> 00:06:59,540
+أفصل و أقوى أكتر من المادة بدي أجيس من الطرف من
+
+101
+00:06:59,540 --> 00:07:03,700
+الخط البداية اللي جيسته من تحت، اللي رسمته من الطرف
+
+102
+00:07:03,700 --> 00:07:08,820
+بدي أجيس واحد سانتي و أحط أول نقطة، و بعدين واحد
+
+103
+00:07:08,820 --> 00:07:12,920
+سانتي و أحط النقطة التاني، و واحد سانتي و أحط
+
+104
+00:07:12,920 --> 00:07:17,140
+النقطة التالتة، فكل نقطة بينها و بين البداية و
+
+105
+00:07:17,140 --> 00:07:20,220
+بينها و بين الجانبها واحد سانتي، بدي أجيس و أحط
+
+106
+00:07:20,220 --> 00:07:25,990
+النقطة، أنا هكذا بكون جهزت الورقة أنه من تحت جسد 8
+
+107
+00:07:25,990 --> 00:07:30,750
+من 10 سانتي و من فوق 5 من 10 سانتي، بعدين على خط
+
+108
+00:07:30,750 --> 00:07:36,030
+البداية اللي أنا رسمته تحت بدي أجيس من الحفة نص
+
+109
+00:07:36,030 --> 00:07:40,880
+سانتي و أحط أول واحد، أول مكوّن، بعدين بدي أجيس بينه
+
+110
+00:07:40,880 --> 00:07:44,260
+وبين التاني واحد سانتي و أحط المكون التاني، و واحد
+
+111
+00:07:44,260 --> 00:07:48,480
+سانتي و أحط المكون التاني، من على الجدار لأول مكون
+
+112
+00:07:48,480 --> 00:07:52,540
+بجيس بس نص سانتي، بس بين المكون و التاني بجيس واحد
+
+113
+00:07:52,540 --> 00:07:56,740
+سانتي، هلجيت الورقة صارت عندي جاهزة زيك و مرسوم
+
+114
+00:07:56,740 --> 00:07:59,640
+الخط البداية و الخط النهائية و أين بدي أحط كل مكون
+
+115
+00:07:59,640 --> 00:08:05,640
+بجيب هذا الخليط و بحط على كل مكان، بكون كتب اسمها
+
+116
+00:08:05,640 --> 00:08:08,860
+مثلا الـ أول مكتشر واحد، مكتشر اتنين، و لو بدي اعمل
+
+117
+00:08:08,860 --> 00:08:13,020
+بس glycine لحال و cysteine لحال و bruline لحال، بحط
+
+118
+00:08:13,020 --> 00:08:17,960
+لكل واحدة في مكان من الـ cup بتاعها، خليت الأول باخد
+
+119
+00:08:17,960 --> 00:08:21,700
+بالـ capillary tube القنبوبة الشعرية و بحط نقطة على
+
+120
+00:08:21,700 --> 00:08:25,220
+مكانها، الأول، التانية بحط بالـ capillary tube و بحط
+
+121
+00:08:25,220 --> 00:08:29,760
+نقطة، هلجيت خلصة الورقة الـ stationary phase جاهزة
+
+122
+00:08:29,760 --> 00:08:34,020
+ومحطوط عليها الخليط، بجيب الـ beaker اللي يحتوي على
+
+123
+00:08:34,020 --> 00:08:40,240
+الـ mobile phase، وبحطها بغمرها يعني الورقة بغمرها في
+
+124
+00:08:40,240 --> 00:08:43,840
+الـ mobile phase، الـ stationary phase اللي هي الورقة
+
+125
+00:08:43,840 --> 00:08:48,660
+بغمرها في الـ mobile phase و بسيبها لحد ما يرتفع
+
+126
+00:08:48,660 --> 00:08:52,820
+السائل في الـ mobile phase، اللي هو الـ mobile phase
+
+127
+00:08:52,820 --> 00:08:57,980
+هذا السائل بدي يرتفع عبر الـ stationary phase، بيرتفع
+
+128
+00:08:57,980 --> 00:09:01,840
+و بيوصل لما يجرب يوصل من خط النهاية بقى أشيل
+
+129
+00:09:01,840 --> 00:09:05,940
+الورقة من الـ mobile face بشيلها من الـ beacon و
+
+130
+00:09:05,940 --> 00:09:11,700
+بطلحها بسيبها تجف في الفرن في الـ oven، بعد ما جف
+
+131
+00:09:11,700 --> 00:09:16,860
+بطلحها، مش هنشوف النقاط مش هنشوف كيف فصل، ليش؟ لأنه
+
+132
+00:09:16,860 --> 00:09:20,440
+أصلا بتكون colorless المواد اللي فصلت أحمد عمانية
+
+133
+00:09:20,440 --> 00:09:24,220
+colorless، ماذا أفعل؟ بجيب الـ developing، بعدما جف
+
+134
+00:09:24,220 --> 00:09:27,260
+بجيب الـ developing solution اللي هو المحلول اللي
+
+135
+00:09:27,260 --> 00:09:30,960
+بيظهر اللون، بقرشه عبارة عن بخاخ، بقرش منه على
+
+136
+00:09:30,960 --> 00:09:36,380
+الورقة و بحطها في الفرن الجاف، بعد ما اتجف ويزود
+
+137
+00:09:36,380 --> 00:09:39,420
+التفاعل، هذا الفرن بين الـ Ninhydrin و المركّب
+
+138
+00:09:39,420 --> 00:09:43,520
+الحمض الأميني، أطلّحها من الفرن بشوف أنه فصل عندي
+
+139
+00:09:43,520 --> 00:09:48,520
+على شكل Spot على شكل نقاط ملونة، كل نقطة في مكان
+
+140
+00:09:48,520 --> 00:09:53,340
+بحسب لكل نقطة الـ retention factor، بدي أجيز المسافة
+
+141
+00:09:53,340 --> 00:09:56,620
+اللي مشيتها النقطة من خط البداية لأين مشيت على
+
+142
+00:09:56,620 --> 00:10:00,920
+النقطة اللي مشي على المسافة اللي مشيها المحلول من
+
+143
+00:10:00,920 --> 00:10:03,480
+البداية للنهاية، اللي مشيها الـ mobile phase من
+
+144
+00:10:03,480 --> 00:10:08,240
+البداية للنهاية، بصير عندي هيك معروف لكل نقطة الـ
+
+145
+00:10:08,240 --> 00:10:11,700
+retention factor، برجع للجدول، هي في عندنا جدول
+
+146
+00:10:11,700 --> 00:10:15,280
+بالاخر بيوضح أنه كل حمض أميني جديش إيه الـ
+
+147
+00:10:15,280 --> 00:10:18,620
+retention factor، أنا بكون حسبت الـ retention factor
+
+148
+00:10:18,620 --> 00:10:24,540
+على فرض طلع معاهي 55%، باجي على هذا الجدول 55% من
+
+149
+00:10:24,540 --> 00:10:27,460
+هو الحمض الأميني الـ tryptophan، إذا هذا هو الـ
+
+150
+00:10:27,460 --> 00:10:32,000
+tryptophan، مثلا طلعت معاهي 5 من 100، من هذا بيطلع الـ
+
+151
+00:10:32,000 --> 00:10:39,370
+arginine، وهكذا، هذه التقنية ككل بيكر انها يحتوي على
+
+152
+00:10:39,370 --> 00:10:42,610
+الـ mobile phase، الـ solvent تحت، وهذا العمود هو
+
+153
+00:10:42,610 --> 00:10:45,910
+عبارة عن الـ stationary phase، بعد ما احط عليها
+
+154
+00:10:45,910 --> 00:10:51,570
+الخليط، بغمرها في داخل الـ mobile phase و بيبدأ يمر
+
+155
+00:10:51,570 --> 00:10:54,770
+الساقل عبرها، لما يصل خط النهائي بطلحها
+
+156
+00:10:55,980 --> 00:10:59,060
+Determination of amino acid using thin layer
+
+157
+00:10:59,060 --> 00:11:03,200
+chromatography، أنا بدي استخدم a thin layer
+
+158
+00:11:03,200 --> 00:11:06,940
+chromatography في هذا المعمل عشان افصل الأحماض
+
+159
+00:11:06,940 --> 00:11:10,480
+الأمنية، خليط من أحماض أمنية، طيب حكينا الأحماض
+
+160
+00:11:10,480 --> 00:11:14,780
+الأمنية colorless، كيف بديك تتعرفي عليها؟ بيحكي اللي
+
+161
+00:11:14,780 --> 00:11:19,160
+بنتعرف عليها باستخدام حاجات عدة مكونات Developing
+
+162
+00:11:19,160 --> 00:11:23,910
+solution، سميناها Developing solution، هاي عبارة عن
+
+163
+00:11:23,910 --> 00:11:27,790
+مادة بتلون الأشياء، فبقدر أعرف بتلونها و بتخليها
+
+164
+00:11:27,790 --> 00:11:31,990
+ملونة و بعرف ما كانت اش، ممكن استخدم اشهر اشي ممكن
+
+165
+00:11:31,990 --> 00:11:35,870
+استخدم مع الـ amino acid هي الـ Ninhydrin Spray، برش
+
+166
+00:11:35,870 --> 00:11:42,190
+من Ninhydrin، بختار بيستخدم كـ visualizing agent، عمل
+
+167
+00:11:42,190 --> 00:11:46,450
+مكشف بقدر اكتشف منه الأحمد الأمينية، انه بلونها
+
+168
+00:11:46,450 --> 00:11:50,350
+حكينا اللي هو مبدأ عمل الـ Ninhydrin، انه بيتكون عند
+
+169
+00:11:50,350 --> 00:11:56,460
+رومانس كمبليكس لونه بيربو، ممكن استخدم iodine vapor
+
+170
+00:11:56,460 --> 00:12:00,600
+as indicator، انه باروش من الـ iodine و هو بيستخدم كـ
+
+171
+00:12:00,600 --> 00
\ No newline at end of file
diff --git a/PL9fwy3NUQKwZ1Q7zOg1ObgufPMjRI3WY1/42Zoe_ECvac_postprocess.srt b/PL9fwy3NUQKwZ1Q7zOg1ObgufPMjRI3WY1/42Zoe_ECvac_postprocess.srt
new file mode 100644
index 0000000000000000000000000000000000000000..76807ad35e68d36524c7cd2e37f386686358e05f
--- /dev/null
+++ b/PL9fwy3NUQKwZ1Q7zOg1ObgufPMjRI3WY1/42Zoe_ECvac_postprocess.srt
@@ -0,0 +1,776 @@
+1
+00:00:00,000 --> 00:00:03,480
+هنبدأ الجهة بالجزء العامل الـ Experiment اللي هي
+
+2
+00:00:03,480 --> 00:00:08,610
+التجربةأولا نحكي عن بعض المصطلحات ال illusion
+
+3
+00:00:08,610 --> 00:00:12,250
+تحكينا هو عبارة عن ال mobile phase تحكينا ال
+
+4
+00:00:12,250 --> 00:00:16,050
+mobile phase ممكن أسميه solvent ممكن أسميه liquid
+
+5
+00:00:16,050 --> 00:00:20,430
+ممكن أسميه illusion ال illusion هي motion of
+
+6
+00:00:20,430 --> 00:00:23,730
+mobile phase through the stationary phase هي حركة
+
+7
+00:00:23,730 --> 00:00:27,250
+ال mobile phase عبر ال stationary phase لما يمشي
+
+8
+00:00:27,250 --> 00:00:30,470
+السائل اللي هو ال mobile phase من الورقة اللي هي
+
+9
+00:00:30,470 --> 00:00:34,400
+ال stationary phase هذه الحركة بسميها illusionالـ
+
+10
+00:00:34,400 --> 00:00:40,480
+illusion time هو الوقت
+
+11
+00:00:40,480 --> 00:00:44,700
+اللذي يحتاجه الـ mobile phase للمعبور عبر
+
+12
+00:00:44,700 --> 00:00:50,060
+الستشريناري phase اللي بيكون له شهر illusion time
+
+13
+00:00:50,450 --> 00:00:54,270
+فهو بيمر أسرع عبر ال stationary phase اللي بيكون
+
+14
+00:00:54,270 --> 00:00:59,150
+وقته أقل أخد واجهة أجال إذا هو ماشي أسرع ال
+
+15
+00:00:59,150 --> 00:01:01,890
+illusion time هو الوقت اللي احتاجه ال mobile phase
+
+16
+00:01:01,890 --> 00:01:05,710
+عشان يمشي عبر ال stationary phase ال mobile phase
+
+17
+00:01:05,710 --> 00:01:09,390
+هو part of a chromatography system that is mobile
+
+18
+00:01:09,390 --> 00:01:13,290
+commonly a solvent mixture زي اللي في ال column
+
+19
+00:01:13,290 --> 00:01:16,730
+chromatography أو thin layer chromatography or gas
+
+20
+00:01:16,730 --> 00:01:21,030
+as gas in gas chromatographyال mobile phase هو جزء
+
+21
+00:01:21,030 --> 00:01:23,970
+من أجزاء ال chromatographic system هما جزءين ال
+
+22
+00:01:23,970 --> 00:01:27,130
+stationary و ال mobile ال mobile هو أحد أجزاءه
+
+23
+00:01:27,130 --> 00:01:30,290
+بيكون عبارة عن solvent عبارة أو ممكن يكون liquid
+
+24
+00:01:30,290 --> 00:01:36,390
+أو gas في تجربتنا لليوم إيش الأدوات اللي بدي
+
+25
+00:01:36,390 --> 00:01:39,650
+استخدمها بدي استخدم اللي you want أكيد بدي mobile
+
+26
+00:01:39,650 --> 00:01:43,230
+phase ال mobile phase اللي هو اللي you wantاللي
+
+27
+00:01:43,230 --> 00:01:47,930
+بنستخدمه اليوم هو عبارة عن unbutanol مع acetic
+
+28
+00:01:47,930 --> 00:01:52,850
+acid مع distilled water نسبة خمسة لواحد لخمسة بحط
+
+29
+00:01:52,850 --> 00:01:56,110
+خمسة من ال butanol على واحد من ال acetic acid على
+
+30
+00:01:56,110 --> 00:02:01,190
+خمسة من ال distilled water و أحركهم لمدة عشر دقايق
+
+31
+00:02:01,190 --> 00:02:05,430
+و بعدين بخليهم يفصلوا لطبقات باخد الطبقة اللي فاج
+
+32
+00:02:05,430 --> 00:02:10,250
+و بستخدمها ك اليوان ك mobile phaseبدي developing
+
+33
+00:02:10,250 --> 00:02:14,490
+solution developing solution هي دي عبارة عن مادة
+
+34
+00:02:14,490 --> 00:02:19,510
+كيميائية عشان توضح لون المادة المفصولة اليوم في
+
+35
+00:02:19,510 --> 00:02:23,570
+تجربتنا بدي اجيب .. بدي نجيب خليط من أحماض أمينية
+
+36
+00:02:23,570 --> 00:02:27,470
+خليط يحتوي على عدة أنواع من الأحماض الأمينية بدي
+
+37
+00:02:27,470 --> 00:02:31,590
+إياه يفصل لمكوناته لإن بعد ما التقنية تمشي ويفصل
+
+38
+00:02:31,590 --> 00:02:36,490
+إلى مكوناته بتكون عندي نقاط في كل .. كل مكون بيفصل
+
+39
+00:02:36,490 --> 00:02:41,550
+في مكان هذه النقاطبتكون colorless بتكون غير ملونة
+
+40
+00:02:41,550 --> 00:02:45,530
+على الورق اللى أنا استخدمتها للفصل ما بيظهر اللون
+
+41
+00:02:45,530 --> 00:02:50,350
+فهتكون مش هشوف النقاط وين فصلت فبدي محلول او مادة
+
+42
+00:02:50,350 --> 00:02:56,270
+تظهرلي اللون بتغير اللون اللى عندي يعني تحولها
+
+43
+00:02:56,270 --> 00:03:01,150
+تظهرليها بملون هتكون بألوان تانية فبدي استخدم مادة
+
+44
+00:03:01,150 --> 00:03:05,200
+بتحددلي اللون المادة المفصولةهذه المادة اللي اليوم
+
+45
+00:03:05,200 --> 00:03:08,500
+نستخدمها اسمها Developing Solution هي عبارة عن
+
+46
+00:03:08,500 --> 00:03:12,020
+تلاتة من عشرة جرام من النهيدرين على عشرة مل من
+
+47
+00:03:12,020 --> 00:03:16,420
+البيوتانول على تلاتة مل من الستيكاسين المغزاة إنه
+
+48
+00:03:16,420 --> 00:03:20,000
+أنا بدي أستخدم محلول يظهر لي لون المادة المفصولة
+
+49
+00:03:20,000 --> 00:03:23,640
+لأنها كانت عبارة عن colorless مالهاش لون فبعد
+
+50
+00:03:23,640 --> 00:03:27,560
+الفصل مش هعرف وين هي وفصلت وين هي وقفت إلا إذا
+
+51
+00:03:27,560 --> 00:03:33,030
+لونتهاأنا اليوم بضفص الأحماض أمينية، تمام؟ الأحماض
+
+52
+00:03:33,030 --> 00:03:35,590
+الأمينية، أيش الفحص اللي بيستخدم لكشف عن كل
+
+53
+00:03:35,590 --> 00:03:38,370
+الأحماض الأمينية؟ مع كل الأحماض الأمينية بيعطيني
+
+54
+00:03:38,370 --> 00:03:42,790
+positive من الفحصات اللي في أول معملة أخدناها كان
+
+55
+00:03:42,790 --> 00:03:46,130
+عبارة عن فحص الـ Nenhydrin الـ Nenhydrin بيعطيني
+
+56
+00:03:46,130 --> 00:03:49,590
+positive مع الـ Free Alpha Amino Group حكينا كلهم
+
+57
+00:03:49,590 --> 00:03:54,210
+عندهم هاي وبيتحول اللون لبربه، مع ذالب رولين عنده
+
+58
+00:03:54,210 --> 00:03:57,410
+amino group بس بيعطيني برضه لون يلون، يعني المغزة
+
+59
+00:03:57,410 --> 00:04:02,220
+هيطلع عندى لونيعني كلهم هيعطوني لهم فهيتلون و أشوف
+
+60
+00:04:02,220 --> 00:04:06,560
+وين هاي ال spot و اكفه إيش كمان بتستخدم؟ بتستخدم
+
+61
+00:04:06,560 --> 00:04:09,740
+solution of different amino acid بد الخليط اللي
+
+62
+00:04:09,740 --> 00:04:15,220
+بيحتوي على عدة مكونات من الأحماض الأمينيةو بدي ال
+
+63
+00:04:15,220 --> 00:04:18,420
+chromatography paper اللي هي ال stationary face و
+
+64
+00:04:18,420 --> 00:04:21,460
+بدي ال lotion chamber ال chamber اللي هو عبارة عن
+
+65
+00:04:21,460 --> 00:04:26,800
+زي بيكر بدي أحط فيه السائل اللي هو عبارة عن ال ال
+
+66
+00:04:26,800 --> 00:04:31,680
+mobile face السائل هدون بدي أحطه بشامبر بيكر glass
+
+67
+00:04:31,680 --> 00:04:36,110
+capillary force booting بدي tubeاللي هى الـ
+
+68
+00:04:36,110 --> 00:04:38,910
+Cabillary Tube القنابيب اللى رفيعة القنابيب
+
+69
+00:04:38,910 --> 00:04:43,550
+الشهرية اظن استخدمتها بالمقدمة هى القنابيب عشان
+
+70
+00:04:43,550 --> 00:04:47,890
+تحطلي ال spot اخد من العينة من الخليط نقطة صغيرة
+
+71
+00:04:47,890 --> 00:04:53,030
+تحطلي مقدار صغير ال drying oven بدي فرن لا تشفيف
+
+72
+00:04:53,030 --> 00:04:57,370
+على درجة حرارة 60 درجة سيليزيا هذا الفرن بس عشان
+
+73
+00:04:57,370 --> 00:05:02,350
+يجفف ويزيد التفاعل بين المواد و ال Nenhydrin بيزود
+
+74
+00:05:02,350 --> 00:05:08,750
+التفاعل المواد مع ال Nenhydrinكيف خطوات العمل؟ كيف
+
+75
+00:05:08,750 --> 00:05:12,130
+أنا بدي أشتغل التجربة؟ هلجيت أن أنا أخد أولا ال
+
+76
+00:05:12,130 --> 00:05:17,270
+stationary fast الورقة تمام هجيت أنا بحكي لإنه لما
+
+77
+00:05:17,270 --> 00:05:22,150
+تشتغلي بدك تكوني لابسة gloves و بدك تستخدمي قلم
+
+78
+00:05:22,150 --> 00:05:26,090
+رصاص في الرسم عليها رسم خط البداية و خط النهاية
+
+79
+00:05:26,090 --> 00:05:29,880
+بدك تستخدمي قلم رصاصليش؟ لأنه جلم الحبر لو
+
+80
+00:05:29,880 --> 00:05:33,260
+استخدمتيه هو عبارة عن أصباغ عبارة عن ألوان لما
+
+81
+00:05:33,260 --> 00:05:36,440
+تيجي تفصليه ممكن تفصل الألوان معاك اللي هو اللي
+
+82
+00:05:36,440 --> 00:05:40,560
+موجودة فيه الجلم الحمر و بدي بس ألبس gloves عشان
+
+83
+00:05:40,560 --> 00:05:44,600
+أتجنب ال contamination ده بعيد عن ال contamination
+
+84
+00:05:44,600 --> 00:05:48,980
+ليش؟ لأن جلدي الإنسان عبارة عن حماض أمنية في الجلب
+
+85
+00:05:48,980 --> 00:05:52,400
+موجودة فانت لو استخدمت الورقة بيدك من غير gloves
+
+86
+00:05:53,090 --> 00:05:56,830
+بصمات اسمك اللى بتحتوي على أحماض أمينية بتطبق على
+
+87
+00:05:56,830 --> 00:06:00,190
+الورقة ممكن تفصل ويصير عندك وانت مانعش ان يصير
+
+88
+00:06:00,190 --> 00:06:04,250
+عندك في أحماض أمينية جاية من الإيد من الجل مش انها
+
+89
+00:06:04,250 --> 00:06:08,830
+من الخليط تمام؟ الجيتكى بده يشتغل بده يجيب ال
+
+90
+00:06:08,830 --> 00:06:12,210
+stationary phase اللى عبارة عن ورقة علنا اليوم
+
+91
+00:06:12,210 --> 00:06:17,560
+stationary phaseالورقة هاي بتكون برس متقبة تمام
+
+92
+00:06:17,560 --> 00:06:23,440
+بدي أعمل فيها خط بداية اللي من عنده بدي أحط الخليط
+
+93
+00:06:23,440 --> 00:06:28,090
+بدي أحط نقطة من المادة اللي بدي أفصلهاكيف بده أحط
+
+94
+00:06:28,090 --> 00:06:31,710
+خط البداية بيحكيلي بنجيس من تحت من أسفل الورقة
+
+95
+00:06:31,710 --> 00:06:37,430
+لفوق بنجيس 8 من 10 سانتي 8 من 10 سانتي من عندها
+
+96
+00:06:37,430 --> 00:06:41,470
+بحط خط البداية إذا من تحت لفوق بدي أجيس 8 من 10
+
+97
+00:06:41,470 --> 00:06:46,590
+سانتي و أرسم خط البداية من فوق من ال end line من
+
+98
+00:06:46,590 --> 00:06:50,790
+خط النهاية لوين بده يوصل من فوق بدي أجيس من فوق 5
+
+99
+00:06:50,790 --> 00:06:56,080
+من 10 سانتيتمام؟ و بعدين إذا عندي أكتر من خليط بدي
+
+100
+00:06:56,080 --> 00:06:59,540
+أفصل و أقوى أكتر من المادة بدي أجيس من الطرف من
+
+101
+00:06:59,540 --> 00:07:03,700
+الخط البداية اللي جيسته من تحت اللي رسمته من الطرف
+
+102
+00:07:03,700 --> 00:07:08,820
+بدي أجيس واحد سانتي و أحط أول نقطة و بعدين واحد
+
+103
+00:07:08,820 --> 00:07:12,920
+سانتي و أحط النقطة التاني و واحد سانتي و أحط
+
+104
+00:07:12,920 --> 00:07:17,140
+النقطة التالتة فكل نقطة بينها و بين البداية و
+
+105
+00:07:17,140 --> 00:07:20,220
+بينها و بين الجانبها واحد سانتي بدي أجيس و أحط
+
+106
+00:07:20,220 --> 00:07:25,990
+النقطةأنا هكذا بكون جهزت الورقة أنه من تحت جسد 8
+
+107
+00:07:25,990 --> 00:07:30,750
+من 10 سانتي و من فوق 5 من 10 سانتي بعدين على خط
+
+108
+00:07:30,750 --> 00:07:36,030
+البداية اللي أنا رسمته تحت بدي أجيس من الحفة نص
+
+109
+00:07:36,030 --> 00:07:40,880
+سانتي و أحط أول واحد أول مكوّنبعدين بدي أجيس بينه
+
+110
+00:07:40,880 --> 00:07:44,260
+وبين التاني واحد سانتي و أحط المكون التاني و واحد
+
+111
+00:07:44,260 --> 00:07:48,480
+سانتي و أحط المكون التاني من على الجدار لأول مكون
+
+112
+00:07:48,480 --> 00:07:52,540
+بجيس بس نص سانتي بس بين المكون و التاني بجيس واحد
+
+113
+00:07:52,540 --> 00:07:56,740
+سانتي هلجيت الورقة صارت عندي جاهزة زيك و مرسوم
+
+114
+00:07:56,740 --> 00:07:59,640
+الخط البداية و الخط النهائية و أين بدي أحط كل مكون
+
+115
+00:07:59,640 --> 00:08:05,640
+بجيب هذا الخليط و بحطعلى كل مكان بكون كتب اسمها
+
+116
+00:08:05,640 --> 00:08:08,860
+مثلا ال اول مكتشر واحد مكتشر اتنين و لو بدي اعمل
+
+117
+00:08:08,860 --> 00:08:13,020
+بس glycine لحال و cysteine لحال و bruline لحال بحط
+
+118
+00:08:13,020 --> 00:08:17,960
+لكل واحدة في مكان من ال cup بتاعها خليت الأول باخد
+
+119
+00:08:17,960 --> 00:08:21,700
+بال capillary tube القنبوبة الشعرية و بحط نقطة على
+
+120
+00:08:21,700 --> 00:08:25,220
+مكانها الأول التانية بحط بال capillary tube و بحط
+
+121
+00:08:25,220 --> 00:08:29,760
+نقطةهلجيت خلصة الورقة ال stationary phase جاهزة
+
+122
+00:08:29,760 --> 00:08:34,020
+ومحطوط عليها الخليط بجيب ال beaker اللي يحتوي على
+
+123
+00:08:34,020 --> 00:08:40,240
+ال mobile phaseوبحطها بغمرها يعني الورقة بغمرها في
+
+124
+00:08:40,240 --> 00:08:43,840
+ال mobile phase ال stationary phase اللي هي الورقة
+
+125
+00:08:43,840 --> 00:08:48,660
+بغمرها في ال mobile phase وبسيبها لحد ما يرتفع
+
+126
+00:08:48,660 --> 00:08:52,820
+السائل في ال mobile phase اللي هو ال mobile phase
+
+127
+00:08:52,820 --> 00:08:57,980
+هذا السائل بدي يرتفع عبر ال stationary phaseبيرتفع
+
+128
+00:08:57,980 --> 00:09:01,840
+و بيوصل لما يجرب يوصل من خط النهاية بقى أشيل
+
+129
+00:09:01,840 --> 00:09:05,940
+الورقة من ال mobile face بشيلها من ال beacon و
+
+130
+00:09:05,940 --> 00:09:11,700
+بطلحها بسيبها جف في الفرن في ال oven بعد ما جف
+
+131
+00:09:11,700 --> 00:09:16,860
+بطلحها مش هنشوف النقاط مش هنشوف كيف فصل ليش لأنه
+
+132
+00:09:16,860 --> 00:09:20,440
+أصلا بتكون colorless المواد اللي فصلت أحمد عمانية
+
+133
+00:09:20,440 --> 00:09:24,220
+colorlessماذا أفعل؟ بجيب الـ developing بعدما جف
+
+134
+00:09:24,220 --> 00:09:27,260
+بجيب الـ developing solution اللي هو المحلول اللي
+
+135
+00:09:27,260 --> 00:09:30,960
+بيظهر اللون بقرشه عبارة عن بخاخ بقرش منه على
+
+136
+00:09:30,960 --> 00:09:36,380
+الورقة و بحطها في الفرن الجافبعد ما اتجف ويزود
+
+137
+00:09:36,380 --> 00:09:39,420
+التفاعل هذا الفرن بين الـ Nenhydrin و المركّب
+
+138
+00:09:39,420 --> 00:09:43,520
+الحمض الأميني أطلّحها من الفرن بشوف أنه فصل عندي
+
+139
+00:09:43,520 --> 00:09:48,520
+على شكل Spot على شكل نقاط ملونة كل نقطة في مكان
+
+140
+00:09:48,520 --> 00:09:53,340
+بحسب لكل نقطة ال retention factor بدي أجيز المسافة
+
+141
+00:09:53,340 --> 00:09:56,620
+اللي مشيتها النقطة من خط البداية لأين مشيت على
+
+142
+00:09:56,620 --> 00:10:00,920
+النقطة اللي مشي على المسافة اللي مشيها المحلول من
+
+143
+00:10:00,920 --> 00:10:03,480
+البداية للنهاية اللي مشيها ال mobile phase من
+
+144
+00:10:03,480 --> 00:10:08,240
+البداية للنهايةبصير عندي هيك معروف لكل نقطة ال
+
+145
+00:10:08,240 --> 00:10:11,700
+retention factor برجع للجدول، هي في عندنا جدول
+
+146
+00:10:11,700 --> 00:10:15,280
+بالاخر بيوضح أنه كل حمض أميني جديش إيه ال
+
+147
+00:10:15,280 --> 00:10:18,620
+retention factor أنا بكون حسبت ال retention factor
+
+148
+00:10:18,620 --> 00:10:24,540
+على فرض طلع معاهي 55% باجي على هذا الجدول 55% من
+
+149
+00:10:24,540 --> 00:10:27,460
+هو الحمض الأميني ال triptofan إذا هذا هو ال
+
+150
+00:10:27,460 --> 00:10:32,000
+triptofan مثلا طلعت معاهي 5 من 100 من هذا بيطلع ال
+
+151
+00:10:32,000 --> 00:10:39,370
+arginine وهكذاهذه التقنية ككل بيكر انها يحتوي على
+
+152
+00:10:39,370 --> 00:10:42,610
+ال mobile phase ال solvent تحت وهذا العمود هو
+
+153
+00:10:42,610 --> 00:10:45,910
+عبارة عن ال stationary phase بعد ما احط عليها
+
+154
+00:10:45,910 --> 00:10:51,570
+الخليط بغمرها في داخل ال mobile phase و بيبدأ يمر
+
+155
+00:10:51,570 --> 00:10:54,770
+الساقل عبرها لما يصل خط النهائي بطلحها
+
+156
+00:10:55,980 --> 00:10:59,060
+Determination of amino acid using thin layer
+
+157
+00:10:59,060 --> 00:11:03,200
+chromatography انا بدي استخدم a thin layer
+
+158
+00:11:03,200 --> 00:11:06,940
+chromatography في هذا المعمل عشان افصل الأحماض
+
+159
+00:11:06,940 --> 00:11:10,480
+الأمنية خليط من أحماض أمنية طيب حكينا الأحماض
+
+160
+00:11:10,480 --> 00:11:14,780
+الأمنية colorless كيف بديك تتعرفي عليها بيحكي اللي
+
+161
+00:11:14,780 --> 00:11:19,160
+بنتعرف عليها باستخدام حاجات عدة مكونات Developing
+
+162
+00:11:19,160 --> 00:11:23,910
+solution سميناها Developing solution هايأبارعاً
+
+163
+00:11:23,910 --> 00:11:27,790
+مادة بتلون الأشياء فبقدر أعرف بتلونها و بتخليها
+
+164
+00:11:27,790 --> 00:11:31,990
+ملونة و بعرف ما كانت اش ممكن استخدم اشهر اشي ممكن
+
+165
+00:11:31,990 --> 00:11:35,870
+استخدم مع ال amino acid هي ال Nihidrin Spray برش
+
+166
+00:11:35,870 --> 00:11:42,190
+من نهيدرين بختار بيستخدم ك visualizing agent عمل
+
+167
+00:11:42,190 --> 00:11:46,450
+مكشف بقدر اكتشف منه الأحمد الأمينية انه بلونها
+
+168
+00:11:46,450 --> 00:11:50,350
+حكينا اللي هو مبدأ عمل ال Nihidrin انه بيتكون عند
+
+169
+00:11:50,350 --> 00:11:56,460
+رومانس كمبليكس لونه بيربو ممكن استخدم iodine vapor
+
+170
+00:11:56,460 --> 00:12:00,600
+as indicator انه باروش من ال iodine و هو بيستخدم ك
+
+171
+00:12:00,600 --> 00:12:04,540
+indicator برضه بيتفاعل مع الحماد الأمنى و ممكن
+
+172
+00:12:04,540 --> 00:12:08,780
+استخدم fluorescence indicator بستخدم صفقة مشعة
+
+173
+00:12:10,950 --> 00:12:15,030
+بترتبط في داخل الأحمد الأمينية وبتعطيني إشعاع
+
+174
+00:12:15,030 --> 00:12:18,990
+باستخدم ultraviolet lamp باللامدة المشعة وين
+
+175
+00:12:18,990 --> 00:12:21,790
+المكان اللي بيشع فيه اللي بيظهر فيه إشعاع إذا هاد
+
+176
+00:12:21,790 --> 00:12:25,870
+عبارة عن حمد أميني فاصل في هذه المنطقة بحسبله
+
+177
+00:12:25,870 --> 00:12:30,130
+retention factor وين ما يعطيني إشعاع تاني مشعق
+
+178
+00:12:30,130 --> 00:12:34,130
+ظهرت إشعاع تحت ال ultraviolet lamp بيكون برضه نقطة
+
+179
+00:12:34,130 --> 00:12:40,650
+حمد أميني آخرهنا عندنا صورة عندنا مثال حط اعطيني
+
+180
+00:12:40,650 --> 00:12:44,530
+على اليمين عندك نقطة الزرقاء خط البداية من نقطة
+
+181
+00:12:44,530 --> 00:12:50,770
+الزرقاء اللي تحت تمام؟ هي لما انفصلت مشيت مسافة من
+
+182
+00:12:50,770 --> 00:12:53,630
+خط البداية من عند وين كانت لو وين وصلت هي اتنين
+
+183
+00:12:53,630 --> 00:12:58,960
+وواحد صاندرالموبايل فاس جدش مشي من البداية للنهاية
+
+184
+00:12:58,960 --> 00:13:03,140
+مشي بيحكي ل 2.8 صندوق كيف بده أحسب ال retention
+
+185
+00:13:03,140 --> 00:13:09,680
+factor؟ بجسم 2 و 1 على 2 و 8 بيطلع 75 من 100 هي ال
+
+186
+00:13:09,680 --> 00:13:13,760
+retention factor لهذه المادة برجع للجدول بشوف من
+
+187
+00:13:13,760 --> 00:13:20,240
+هذه ال 75% من هي المادة و بطلع اسمهاهنا نفس الشيء
+
+188
+00:13:20,240 --> 00:13:23,940
+كيف بدأ أحسب ال retention factor اللي هنا بمثلها
+
+189
+00:13:23,940 --> 00:13:28,260
+بي على ا برموز بي على ا اللي هي المسافة اللي
+
+190
+00:13:28,260 --> 00:13:31,880
+قطعتها المادة من البداية لون وصلت على المسافة اللي
+
+191
+00:13:31,880 --> 00:13:36,280
+قطعها ال solvent من البداية للنهاية هنا جدول فيه
+
+192
+00:13:36,280 --> 00:13:40,340
+بعض الأحمد الأمينية و retention factor لكل حمض
+
+193
+00:13:40,340 --> 00:13:47,560
+أميني وهذا هو كل المعمل لهذه التجربة أتمنى تكونوا
+
+194
+00:13:47,560 --> 00:13:48,180
+فهمتها
+
diff --git a/PL9fwy3NUQKwZ1Q7zOg1ObgufPMjRI3WY1/42Zoe_ECvac_raw.json b/PL9fwy3NUQKwZ1Q7zOg1ObgufPMjRI3WY1/42Zoe_ECvac_raw.json
new file mode 100644
index 0000000000000000000000000000000000000000..687c069e3e9de1df11310bce64806339676ad99d
--- /dev/null
+++ b/PL9fwy3NUQKwZ1Q7zOg1ObgufPMjRI3WY1/42Zoe_ECvac_raw.json
@@ -0,0 +1 @@
+{"segments": [{"id": 1, "seek": 416, "start": 0.0, "end": 4.16, "text": "هنبدأ الجهة بالجزء العامل الـ Experiment اللي هي التجربة", "tokens": [3224, 1863, 44510, 10721, 25724, 3224, 3660, 20666, 7435, 11622, 38207, 18863, 10943, 1211, 2423, 39184, 37933, 13672, 1829, 39896, 16712, 7435, 25513, 3660], "avg_logprob": -0.3085937404632568, "compression_ratio": 1.146341463414634, "no_speech_prob": 0.0, "words": [{"start": 0.0, "end": 1.04, "word": "هنبدأ", "probability": 0.9041748046875}, {"start": 1.04, "end": 1.36, "word": " الجهة", "probability": 0.55419921875}, {"start": 1.36, "end": 1.84, "word": " بالجزء", "probability": 0.8135986328125}, {"start": 1.84, "end": 2.24, "word": " العامل", "probability": 0.7919921875}, {"start": 2.24, "end": 2.46, "word": " الـ", "probability": 0.642333984375}, {"start": 2.46, "end": 2.96, "word": " Experiment", "probability": 0.7548828125}, {"start": 2.96, "end": 3.4, "word": " اللي", "probability": 0.5760498046875}, {"start": 3.4, "end": 3.48, "word": " هي", "probability": 0.80810546875}, {"start": 3.48, "end": 4.16, "word": " التجربة", "probability": 0.9642333984375}], "temperature": 1.0}, {"id": 2, "seek": 3333, "start": 5.09, "end": 33.33, "text": "أولا نحكي عن بعض المصطلحات ال illusion تحكينا هو عبارة عن ال mobile phase تحكينا ال mobile phase ممكن أسميه solvent ممكن أسميه liquid ممكن أسميه illusion ال illusion هي motion of mobile phase through the stationary phase هي حركة ال mobile phase عبر ال stationary phase لما يمشي السائل اللي هو ال mobile phase من الورقة اللي هي ال stationary phase هذه الحركة بسميها illusion", "tokens": [10721, 12610, 995, 8717, 5016, 4117, 1829, 18871, 45030, 11242, 9673, 9381, 9566, 1211, 5016, 9307, 2423, 18854, 6055, 5016, 4117, 1829, 8315, 31439, 6225, 3555, 9640, 3660, 18871, 2423, 6013, 5574, 6055, 5016, 4117, 1829, 8315, 2423, 6013, 5574, 3714, 43020, 5551, 38251, 1829, 3224, 33575, 3714, 43020, 5551, 38251, 1829, 3224, 6553, 3714, 43020, 5551, 38251, 1829, 3224, 18854, 2423, 18854, 39896, 5394, 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"probability": 0.9444580078125}, {"start": 769.09, "end": 769.41, "word": " مشيت", "probability": 0.80517578125}, {"start": 769.41, "end": 770.23, "word": " مسافة", "probability": 0.97705078125}, {"start": 770.23, "end": 770.77, "word": " من", "probability": 0.99462890625}, {"start": 770.77, "end": 771.03, "word": " خط", "probability": 0.975341796875}, {"start": 771.03, "end": 771.57, "word": " البداية", "probability": 0.9703776041666666}, {"start": 771.57, "end": 771.71, "word": " من", "probability": 0.72509765625}, {"start": 771.71, "end": 771.89, "word": " عند", "probability": 0.6728515625}, {"start": 771.89, "end": 772.09, "word": " وين", "probability": 0.74853515625}, {"start": 772.09, "end": 772.51, "word": " كانت", "probability": 0.99462890625}, {"start": 772.51, "end": 772.63, "word": " لو", "probability": 0.278564453125}, {"start": 772.63, "end": 772.75, "word": " وين", "probability": 0.85498046875}, {"start": 772.75, "end": 773.17, "word": " وصلت", "probability": 0.9856770833333334}, {"start": 773.17, "end": 773.29, "word": " هي", "probability": 0.8984375}, {"start": 773.29, "end": 773.63, "word": " اتنين", "probability": 0.8726806640625}, {"start": 773.63, "end": 774.03, "word": " وواحد", "probability": 0.8466796875}, {"start": 774.03, "end": 774.57, "word": " صاندر", "probability": 0.74383544921875}], "temperature": 1.0}, {"id": 35, "seek": 79726, "start": 775.44, "end": 797.26, "text": "الموبايل فاس جدش مشي من البداية للنهاية مشي بيحكي ل 2.8 صندوق كيف بده أحسب ال retention factor؟ بجسم 2 و 1 على 2 و 8 بيطلع 75 من 100 هي ال retention factor لهذه المادة برجع للجدول بشوف من هذه ال 75% من هي المادة و بطلع اسمها", "tokens": [45340, 37746, 995, 26895, 6156, 32277, 10874, 3215, 8592, 37893, 1829, 9154, 29739, 28259, 10632, 24976, 1863, 11296, 10632, 37893, 1829, 4724, 1829, 5016, 4117, 1829, 5296, 568, 13, 23, 20328, 41260, 30543, 9122, 33911, 47525, 3224, 5551, 5016, 35457, 2423, 22871, 5952, 22807, 4724, 7435, 38251, 568, 4032, 502, 15844, 568, 4032, 1649, 4724, 1829, 9566, 1211, 3615, 9562, 9154, 2319, 39896, 2423, 22871, 5952, 46740, 24192, 9673, 18513, 3660, 4724, 47341, 3615, 24976, 7435, 3215, 12610, 4724, 8592, 38688, 9154, 29538, 2423, 9562, 4, 9154, 39896, 9673, 18513, 3660, 4032, 4724, 9566, 1211, 3615, 24525, 2304, 11296], "avg_logprob": -0.2165625, "compression_ratio": 1.628440366972477, "no_speech_prob": 0.0, "words": [{"start": 775.44, "end": 776.1, "word": "الموبايل", "probability": 0.740020751953125}, {"start": 776.1, "end": 776.38, "word": " فاس", "probability": 0.3856201171875}, {"start": 776.38, "end": 776.74, "word": " جدش", "probability": 0.7360026041666666}, {"start": 776.74, "end": 777.12, "word": " مشي", "probability": 0.654052734375}, {"start": 777.12, "end": 777.3, "word": " من", "probability": 0.9921875}, {"start": 777.3, "end": 777.98, "word": " البداية", "probability": 0.9786783854166666}, {"start": 777.98, "end": 778.96, "word": " للنهاية", "probability": 0.80377197265625}, {"start": 778.96, "end": 779.58, "word": " مشي", "probability": 0.9248046875}, {"start": 779.58, "end": 779.96, "word": " بيحكي", "probability": 0.98935546875}, {"start": 779.96, "end": 780.08, "word": " ل", "probability": 0.5166015625}, {"start": 780.08, "end": 780.42, "word": " 2", "probability": 0.56787109375}, {"start": 780.42, "end": 781.32, "word": ".8", "probability": 0.969970703125}, {"start": 781.32, "end": 781.96, "word": " صندوق", "probability": 0.74560546875}, {"start": 781.96, "end": 782.32, "word": " كيف", "probability": 0.774169921875}, {"start": 782.32, "end": 782.52, "word": " بده", "probability": 0.4969482421875}, {"start": 782.52, "end": 782.74, "word": " أحسب", "probability": 0.9265950520833334}, {"start": 782.74, "end": 782.84, "word": " ال", "probability": 0.9775390625}, {"start": 782.84, "end": 783.14, "word": " retention", "probability": 0.9423828125}, {"start": 783.14, "end": 783.98, "word": " factor؟", "probability": 0.760498046875}, {"start": 783.98, "end": 784.64, "word": " بجسم", "probability": 0.7129720052083334}, {"start": 784.64, "end": 785.02, "word": " 2", "probability": 0.67822265625}, {"start": 785.02, "end": 785.18, "word": " و", "probability": 0.68408203125}, {"start": 785.18, "end": 785.64, "word": " 1", "probability": 0.810546875}, {"start": 785.64, "end": 786.0, "word": " على", "probability": 0.68603515625}, {"start": 786.0, "end": 786.58, "word": " 2", "probability": 0.98291015625}, {"start": 786.58, "end": 786.78, "word": " و", "probability": 0.88623046875}, {"start": 786.78, "end": 787.2, "word": " 8", "probability": 0.953125}, {"start": 787.2, "end": 788.0, "word": " بيطلع", "probability": 0.9533203125}, {"start": 788.0, "end": 788.7, "word": " 75", "probability": 0.9619140625}, {"start": 788.7, "end": 788.94, "word": " من", "probability": 0.685546875}, {"start": 788.94, "end": 789.26, "word": " 100", "probability": 0.80224609375}, {"start": 789.26, "end": 789.58, "word": " هي", "probability": 0.309814453125}, {"start": 789.58, "end": 789.68, "word": " ال", "probability": 0.58203125}, {"start": 789.68, "end": 789.96, "word": " retention", "probability": 0.99609375}, {"start": 789.96, "end": 790.46, "word": " factor", "probability": 0.94287109375}, {"start": 790.46, "end": 790.88, "word": " لهذه", "probability": 0.82470703125}, {"start": 790.88, "end": 791.62, "word": " المادة", "probability": 0.9925130208333334}, {"start": 791.62, "end": 792.44, "word": " برجع", "probability": 0.95068359375}, {"start": 792.44, "end": 793.08, "word": " للجدول", "probability": 0.974365234375}, {"start": 793.08, "end": 793.5, "word": " بشوف", "probability": 0.9508463541666666}, {"start": 793.5, "end": 793.76, "word": " من", "probability": 0.8193359375}, {"start": 793.76, "end": 794.0, "word": " هذه", "probability": 0.67236328125}, {"start": 794.0, "end": 794.1, "word": " ال", "probability": 0.92041015625}, {"start": 794.1, "end": 794.64, "word": " 75", "probability": 0.88818359375}, {"start": 794.64, "end": 795.22, "word": "%", "probability": 0.943359375}, {"start": 795.22, "end": 795.44, "word": " من", "probability": 0.69580078125}, {"start": 795.44, "end": 795.62, "word": " هي", "probability": 0.5576171875}, {"start": 795.62, "end": 796.1, "word": " المادة", "probability": 0.99462890625}, {"start": 796.1, "end": 796.2, "word": " و", "probability": 0.6318359375}, {"start": 796.2, "end": 796.68, "word": " بطلع", "probability": 0.9296875}, {"start": 796.68, "end": 797.26, "word": " اسمها", "probability": 0.833984375}], "temperature": 1.0}, {"id": 36, "seek": 82818, "start": 799.06, "end": 828.18, "text": "هنا نفس الشيء كيف بدأ أحسب ال retention factor اللي هنا بمثلها بي على ا برموز بي على ا اللي هي المسافة اللي قطعتها المادة من البداية لون وصلت على المسافة اللي قطعها ال solvent من البداية للنهاية هنا جدول فيه بعض الأحمد الأمينية و retention factor لكل حمض أميني وهذا هو كل المعمل لهذه التجربة أتمنى تكونوا فهمتها", "tokens": [3224, 8315, 8717, 36178, 25124, 1829, 38207, 9122, 33911, 47525, 10721, 5551, 5016, 35457, 2423, 22871, 5952, 13672, 1829, 34105, 4724, 2304, 12984, 1211, 11296, 4724, 1829, 15844, 1975, 4724, 2288, 2304, 2407, 11622, 4724, 1829, 15844, 1975, 13672, 1829, 39896, 9673, 3794, 31845, 3660, 13672, 1829, 12174, 9566, 34268, 11296, 9673, 18513, 3660, 9154, 29739, 28259, 10632, 5296, 11536, 4032, 36520, 2655, 15844, 9673, 3794, 31845, 3660, 13672, 1829, 12174, 9566, 3615, 11296, 2423, 33575, 9154, 29739, 28259, 10632, 24976, 1863, 11296, 10632, 34105, 10874, 3215, 12610, 8978, 3224, 45030, 11242, 16247, 35571, 3215, 16247, 2304, 9957, 10632, 4032, 22871, 5952, 5296, 28820, 11331, 2304, 11242, 5551, 2304, 9957, 1829, 37037, 15730, 31439, 28242, 9673, 25957, 1211, 46740, 24192, 16712, 7435, 25513, 3660, 5551, 2655, 27842, 7578, 6055, 30544, 14407, 6156, 16095, 2655, 11296], "avg_logprob": -0.1821001843713662, "compression_ratio": 2.026923076923077, "no_speech_prob": 0.0, "words": [{"start": 799.06, "end": 799.4, "word": "هنا", "probability": 0.908203125}, {"start": 799.4, "end": 799.68, "word": " نفس", "probability": 0.975341796875}, {"start": 799.68, "end": 800.24, "word": " الشيء", "probability": 0.7799479166666666}, {"start": 800.24, "end": 800.46, "word": " كيف", "probability": 0.91796875}, {"start": 800.46, "end": 800.7, "word": " بدأ", "probability": 0.6217041015625}, {"start": 800.7, "end": 801.06, "word": " أحسب", "probability": 0.8914388020833334}, {"start": 801.06, "end": 801.28, "word": " ال", "probability": 0.86572265625}, {"start": 801.28, "end": 801.58, "word": " retention", "probability": 0.92626953125}, {"start": 801.58, "end": 802.3, "word": " factor", "probability": 0.9267578125}, {"start": 802.3, "end": 802.9, "word": " اللي", "probability": 0.7275390625}, {"start": 802.9, "end": 803.22, "word": " هنا", "probability": 0.37255859375}, {"start": 803.22, "end": 803.94, "word": " بمثلها", "probability": 0.778466796875}, {"start": 803.94, "end": 804.82, "word": " بي", "probability": 0.5189208984375}, {"start": 804.82, "end": 805.12, "word": " على", "probability": 0.787109375}, {"start": 805.12, "end": 805.36, "word": " ا", "probability": 0.369140625}, {"start": 805.36, "end": 805.98, "word": " برموز", "probability": 0.84765625}, {"start": 805.98, "end": 806.6, "word": " بي", "probability": 0.806396484375}, {"start": 806.6, "end": 807.0, "word": " على", "probability": 0.89453125}, {"start": 807.0, "end": 807.24, "word": " ا", "probability": 0.7763671875}, {"start": 807.24, "end": 807.46, "word": " اللي", "probability": 0.889892578125}, {"start": 807.46, "end": 807.56, "word": " هي", "probability": 0.7646484375}, {"start": 807.56, "end": 808.1, "word": " المسافة", "probability": 0.97314453125}, {"start": 808.1, "end": 808.26, "word": " اللي", "probability": 0.96044921875}, {"start": 808.26, "end": 808.82, "word": " قطعتها", "probability": 0.9818115234375}, {"start": 808.82, "end": 809.24, "word": " المادة", "probability": 0.9788411458333334}, {"start": 809.24, "end": 809.4, "word": " من", "probability": 0.98876953125}, {"start": 809.4, "end": 809.84, "word": " البداية", "probability": 0.9713541666666666}, {"start": 809.84, "end": 810.12, "word": " لون", "probability": 0.4239501953125}, {"start": 810.12, "end": 810.64, "word": " وصلت", "probability": 0.9664713541666666}, {"start": 810.64, "end": 811.18, "word": " على", "probability": 0.6826171875}, {"start": 811.18, "end": 811.7, "word": " المسافة", "probability": 0.9814453125}, {"start": 811.7, "end": 811.88, "word": " اللي", "probability": 0.990966796875}, {"start": 811.88, "end": 812.32, "word": " قطعها", "probability": 0.9261474609375}, {"start": 812.32, "end": 812.52, "word": " ال", "probability": 0.71240234375}, {"start": 812.52, "end": 812.8, "word": " solvent", "probability": 0.859375}, {"start": 812.8, "end": 813.12, "word": " من", "probability": 0.96875}, {"start": 813.12, "end": 813.7, "word": " البداية", "probability": 0.9890950520833334}, {"start": 813.7, "end": 814.52, "word": " للنهاية", "probability": 0.879150390625}, {"start": 814.52, "end": 815.46, "word": " هنا", "probability": 0.947265625}, {"start": 815.46, "end": 815.96, "word": " جدول", "probability": 0.9680989583333334}, {"start": 815.96, "end": 816.28, "word": " فيه", "probability": 0.7958984375}, {"start": 816.28, "end": 816.54, "word": " بعض", "probability": 0.963134765625}, {"start": 816.54, "end": 817.0, "word": " الأحمد", "probability": 0.8264973958333334}, {"start": 817.0, "end": 817.6, "word": " الأمينية", "probability": 0.9097900390625}, {"start": 817.6, "end": 817.8, "word": " و", "probability": 0.80419921875}, {"start": 817.8, "end": 818.14, "word": " retention", "probability": 0.50634765625}, {"start": 818.14, "end": 818.94, "word": " factor", "probability": 0.921875}, {"start": 818.94, "end": 819.82, "word": " لكل", "probability": 0.976318359375}, {"start": 819.82, "end": 820.34, "word": " حمض", "probability": 0.9296875}, {"start": 820.34, "end": 821.18, "word": " أميني", "probability": 0.9246826171875}, {"start": 821.18, "end": 822.54, "word": " وهذا", "probability": 0.60302734375}, {"start": 822.54, "end": 822.82, "word": " هو", "probability": 0.82861328125}, {"start": 822.82, "end": 823.22, "word": " كل", "probability": 0.96044921875}, {"start": 823.22, "end": 824.18, "word": " المعمل", "probability": 0.9407552083333334}, {"start": 824.18, "end": 825.16, "word": " لهذه", "probability": 0.7061767578125}, {"start": 825.16, "end": 826.78, "word": " التجربة", "probability": 0.9439697265625}, {"start": 826.78, "end": 827.08, "word": " أتمنى", "probability": 0.714508056640625}, {"start": 827.08, "end": 827.56, "word": " تكونوا", "probability": 0.85546875}, {"start": 827.56, "end": 828.18, "word": " فهمتها", "probability": 0.7945556640625}], "temperature": 1.0}], "language": "ar", "language_probability": 1.0, "duration": 828.975625, "duration_after_vad": 809.4853437500013}
\ No newline at end of file
diff --git a/PL9fwy3NUQKwZ1Q7zOg1ObgufPMjRI3WY1/42Zoe_ECvac_raw.srt b/PL9fwy3NUQKwZ1Q7zOg1ObgufPMjRI3WY1/42Zoe_ECvac_raw.srt
new file mode 100644
index 0000000000000000000000000000000000000000..76807ad35e68d36524c7cd2e37f386686358e05f
--- /dev/null
+++ b/PL9fwy3NUQKwZ1Q7zOg1ObgufPMjRI3WY1/42Zoe_ECvac_raw.srt
@@ -0,0 +1,776 @@
+1
+00:00:00,000 --> 00:00:03,480
+هنبدأ الجهة بالجزء العامل الـ Experiment اللي هي
+
+2
+00:00:03,480 --> 00:00:08,610
+التجربةأولا نحكي عن بعض المصطلحات ال illusion
+
+3
+00:00:08,610 --> 00:00:12,250
+تحكينا هو عبارة عن ال mobile phase تحكينا ال
+
+4
+00:00:12,250 --> 00:00:16,050
+mobile phase ممكن أسميه solvent ممكن أسميه liquid
+
+5
+00:00:16,050 --> 00:00:20,430
+ممكن أسميه illusion ال illusion هي motion of
+
+6
+00:00:20,430 --> 00:00:23,730
+mobile phase through the stationary phase هي حركة
+
+7
+00:00:23,730 --> 00:00:27,250
+ال mobile phase عبر ال stationary phase لما يمشي
+
+8
+00:00:27,250 --> 00:00:30,470
+السائل اللي هو ال mobile phase من الورقة اللي هي
+
+9
+00:00:30,470 --> 00:00:34,400
+ال stationary phase هذه الحركة بسميها illusionالـ
+
+10
+00:00:34,400 --> 00:00:40,480
+illusion time هو الوقت
+
+11
+00:00:40,480 --> 00:00:44,700
+اللذي يحتاجه الـ mobile phase للمعبور عبر
+
+12
+00:00:44,700 --> 00:00:50,060
+الستشريناري phase اللي بيكون له شهر illusion time
+
+13
+00:00:50,450 --> 00:00:54,270
+فهو بيمر أسرع عبر ال stationary phase اللي بيكون
+
+14
+00:00:54,270 --> 00:00:59,150
+وقته أقل أخد واجهة أجال إذا هو ماشي أسرع ال
+
+15
+00:00:59,150 --> 00:01:01,890
+illusion time هو الوقت اللي احتاجه ال mobile phase
+
+16
+00:01:01,890 --> 00:01:05,710
+عشان يمشي عبر ال stationary phase ال mobile phase
+
+17
+00:01:05,710 --> 00:01:09,390
+هو part of a chromatography system that is mobile
+
+18
+00:01:09,390 --> 00:01:13,290
+commonly a solvent mixture زي اللي في ال column
+
+19
+00:01:13,290 --> 00:01:16,730
+chromatography أو thin layer chromatography or gas
+
+20
+00:01:16,730 --> 00:01:21,030
+as gas in gas chromatographyال mobile phase هو جزء
+
+21
+00:01:21,030 --> 00:01:23,970
+من أجزاء ال chromatographic system هما جزءين ال
+
+22
+00:01:23,970 --> 00:01:27,130
+stationary و ال mobile ال mobile هو أحد أجزاءه
+
+23
+00:01:27,130 --> 00:01:30,290
+بيكون عبارة عن solvent عبارة أو ممكن يكون liquid
+
+24
+00:01:30,290 --> 00:01:36,390
+أو gas في تجربتنا لليوم إيش الأدوات اللي بدي
+
+25
+00:01:36,390 --> 00:01:39,650
+استخدمها بدي استخدم اللي you want أكيد بدي mobile
+
+26
+00:01:39,650 --> 00:01:43,230
+phase ال mobile phase اللي هو اللي you wantاللي
+
+27
+00:01:43,230 --> 00:01:47,930
+بنستخدمه اليوم هو عبارة عن unbutanol مع acetic
+
+28
+00:01:47,930 --> 00:01:52,850
+acid مع distilled water نسبة خمسة لواحد لخمسة بحط
+
+29
+00:01:52,850 --> 00:01:56,110
+خمسة من ال butanol على واحد من ال acetic acid على
+
+30
+00:01:56,110 --> 00:02:01,190
+خمسة من ال distilled water و أحركهم لمدة عشر دقايق
+
+31
+00:02:01,190 --> 00:02:05,430
+و بعدين بخليهم يفصلوا لطبقات باخد الطبقة اللي فاج
+
+32
+00:02:05,430 --> 00:02:10,250
+و بستخدمها ك اليوان ك mobile phaseبدي developing
+
+33
+00:02:10,250 --> 00:02:14,490
+solution developing solution هي دي عبارة عن مادة
+
+34
+00:02:14,490 --> 00:02:19,510
+كيميائية عشان توضح لون المادة المفصولة اليوم في
+
+35
+00:02:19,510 --> 00:02:23,570
+تجربتنا بدي اجيب .. بدي نجيب خليط من أحماض أمينية
+
+36
+00:02:23,570 --> 00:02:27,470
+خليط يحتوي على عدة أنواع من الأحماض الأمينية بدي
+
+37
+00:02:27,470 --> 00:02:31,590
+إياه يفصل لمكوناته لإن بعد ما التقنية تمشي ويفصل
+
+38
+00:02:31,590 --> 00:02:36,490
+إلى مكوناته بتكون عندي نقاط في كل .. كل مكون بيفصل
+
+39
+00:02:36,490 --> 00:02:41,550
+في مكان هذه النقاطبتكون colorless بتكون غير ملونة
+
+40
+00:02:41,550 --> 00:02:45,530
+على الورق اللى أنا استخدمتها للفصل ما بيظهر اللون
+
+41
+00:02:45,530 --> 00:02:50,350
+فهتكون مش هشوف النقاط وين فصلت فبدي محلول او مادة
+
+42
+00:02:50,350 --> 00:02:56,270
+تظهرلي اللون بتغير اللون اللى عندي يعني تحولها
+
+43
+00:02:56,270 --> 00:03:01,150
+تظهرليها بملون هتكون بألوان تانية فبدي استخدم مادة
+
+44
+00:03:01,150 --> 00:03:05,200
+بتحددلي اللون المادة المفصولةهذه المادة اللي اليوم
+
+45
+00:03:05,200 --> 00:03:08,500
+نستخدمها اسمها Developing Solution هي عبارة عن
+
+46
+00:03:08,500 --> 00:03:12,020
+تلاتة من عشرة جرام من النهيدرين على عشرة مل من
+
+47
+00:03:12,020 --> 00:03:16,420
+البيوتانول على تلاتة مل من الستيكاسين المغزاة إنه
+
+48
+00:03:16,420 --> 00:03:20,000
+أنا بدي أستخدم محلول يظهر لي لون المادة المفصولة
+
+49
+00:03:20,000 --> 00:03:23,640
+لأنها كانت عبارة عن colorless مالهاش لون فبعد
+
+50
+00:03:23,640 --> 00:03:27,560
+الفصل مش هعرف وين هي وفصلت وين هي وقفت إلا إذا
+
+51
+00:03:27,560 --> 00:03:33,030
+لونتهاأنا اليوم بضفص الأحماض أمينية، تمام؟ الأحماض
+
+52
+00:03:33,030 --> 00:03:35,590
+الأمينية، أيش الفحص اللي بيستخدم لكشف عن كل
+
+53
+00:03:35,590 --> 00:03:38,370
+الأحماض الأمينية؟ مع كل الأحماض الأمينية بيعطيني
+
+54
+00:03:38,370 --> 00:03:42,790
+positive من الفحصات اللي في أول معملة أخدناها كان
+
+55
+00:03:42,790 --> 00:03:46,130
+عبارة عن فحص الـ Nenhydrin الـ Nenhydrin بيعطيني
+
+56
+00:03:46,130 --> 00:03:49,590
+positive مع الـ Free Alpha Amino Group حكينا كلهم
+
+57
+00:03:49,590 --> 00:03:54,210
+عندهم هاي وبيتحول اللون لبربه، مع ذالب رولين عنده
+
+58
+00:03:54,210 --> 00:03:57,410
+amino group بس بيعطيني برضه لون يلون، يعني المغزة
+
+59
+00:03:57,410 --> 00:04:02,220
+هيطلع عندى لونيعني كلهم هيعطوني لهم فهيتلون و أشوف
+
+60
+00:04:02,220 --> 00:04:06,560
+وين هاي ال spot و اكفه إيش كمان بتستخدم؟ بتستخدم
+
+61
+00:04:06,560 --> 00:04:09,740
+solution of different amino acid بد الخليط اللي
+
+62
+00:04:09,740 --> 00:04:15,220
+بيحتوي على عدة مكونات من الأحماض الأمينيةو بدي ال
+
+63
+00:04:15,220 --> 00:04:18,420
+chromatography paper اللي هي ال stationary face و
+
+64
+00:04:18,420 --> 00:04:21,460
+بدي ال lotion chamber ال chamber اللي هو عبارة عن
+
+65
+00:04:21,460 --> 00:04:26,800
+زي بيكر بدي أحط فيه السائل اللي هو عبارة عن ال ال
+
+66
+00:04:26,800 --> 00:04:31,680
+mobile face السائل هدون بدي أحطه بشامبر بيكر glass
+
+67
+00:04:31,680 --> 00:04:36,110
+capillary force booting بدي tubeاللي هى الـ
+
+68
+00:04:36,110 --> 00:04:38,910
+Cabillary Tube القنابيب اللى رفيعة القنابيب
+
+69
+00:04:38,910 --> 00:04:43,550
+الشهرية اظن استخدمتها بالمقدمة هى القنابيب عشان
+
+70
+00:04:43,550 --> 00:04:47,890
+تحطلي ال spot اخد من العينة من الخليط نقطة صغيرة
+
+71
+00:04:47,890 --> 00:04:53,030
+تحطلي مقدار صغير ال drying oven بدي فرن لا تشفيف
+
+72
+00:04:53,030 --> 00:04:57,370
+على درجة حرارة 60 درجة سيليزيا هذا الفرن بس عشان
+
+73
+00:04:57,370 --> 00:05:02,350
+يجفف ويزيد التفاعل بين المواد و ال Nenhydrin بيزود
+
+74
+00:05:02,350 --> 00:05:08,750
+التفاعل المواد مع ال Nenhydrinكيف خطوات العمل؟ كيف
+
+75
+00:05:08,750 --> 00:05:12,130
+أنا بدي أشتغل التجربة؟ هلجيت أن أنا أخد أولا ال
+
+76
+00:05:12,130 --> 00:05:17,270
+stationary fast الورقة تمام هجيت أنا بحكي لإنه لما
+
+77
+00:05:17,270 --> 00:05:22,150
+تشتغلي بدك تكوني لابسة gloves و بدك تستخدمي قلم
+
+78
+00:05:22,150 --> 00:05:26,090
+رصاص في الرسم عليها رسم خط البداية و خط النهاية
+
+79
+00:05:26,090 --> 00:05:29,880
+بدك تستخدمي قلم رصاصليش؟ لأنه جلم الحبر لو
+
+80
+00:05:29,880 --> 00:05:33,260
+استخدمتيه هو عبارة عن أصباغ عبارة عن ألوان لما
+
+81
+00:05:33,260 --> 00:05:36,440
+تيجي تفصليه ممكن تفصل الألوان معاك اللي هو اللي
+
+82
+00:05:36,440 --> 00:05:40,560
+موجودة فيه الجلم الحمر و بدي بس ألبس gloves عشان
+
+83
+00:05:40,560 --> 00:05:44,600
+أتجنب ال contamination ده بعيد عن ال contamination
+
+84
+00:05:44,600 --> 00:05:48,980
+ليش؟ لأن جلدي الإنسان عبارة عن حماض أمنية في الجلب
+
+85
+00:05:48,980 --> 00:05:52,400
+موجودة فانت لو استخدمت الورقة بيدك من غير gloves
+
+86
+00:05:53,090 --> 00:05:56,830
+بصمات اسمك اللى بتحتوي على أحماض أمينية بتطبق على
+
+87
+00:05:56,830 --> 00:06:00,190
+الورقة ممكن تفصل ويصير عندك وانت مانعش ان يصير
+
+88
+00:06:00,190 --> 00:06:04,250
+عندك في أحماض أمينية جاية من الإيد من الجل مش انها
+
+89
+00:06:04,250 --> 00:06:08,830
+من الخليط تمام؟ الجيتكى بده يشتغل بده يجيب ال
+
+90
+00:06:08,830 --> 00:06:12,210
+stationary phase اللى عبارة عن ورقة علنا اليوم
+
+91
+00:06:12,210 --> 00:06:17,560
+stationary phaseالورقة هاي بتكون برس متقبة تمام
+
+92
+00:06:17,560 --> 00:06:23,440
+بدي أعمل فيها خط بداية اللي من عنده بدي أحط الخليط
+
+93
+00:06:23,440 --> 00:06:28,090
+بدي أحط نقطة من المادة اللي بدي أفصلهاكيف بده أحط
+
+94
+00:06:28,090 --> 00:06:31,710
+خط البداية بيحكيلي بنجيس من تحت من أسفل الورقة
+
+95
+00:06:31,710 --> 00:06:37,430
+لفوق بنجيس 8 من 10 سانتي 8 من 10 سانتي من عندها
+
+96
+00:06:37,430 --> 00:06:41,470
+بحط خط البداية إذا من تحت لفوق بدي أجيس 8 من 10
+
+97
+00:06:41,470 --> 00:06:46,590
+سانتي و أرسم خط البداية من فوق من ال end line من
+
+98
+00:06:46,590 --> 00:06:50,790
+خط النهاية لوين بده يوصل من فوق بدي أجيس من فوق 5
+
+99
+00:06:50,790 --> 00:06:56,080
+من 10 سانتيتمام؟ و بعدين إذا عندي أكتر من خليط بدي
+
+100
+00:06:56,080 --> 00:06:59,540
+أفصل و أقوى أكتر من المادة بدي أجيس من الطرف من
+
+101
+00:06:59,540 --> 00:07:03,700
+الخط البداية اللي جيسته من تحت اللي رسمته من الطرف
+
+102
+00:07:03,700 --> 00:07:08,820
+بدي أجيس واحد سانتي و أحط أول نقطة و بعدين واحد
+
+103
+00:07:08,820 --> 00:07:12,920
+سانتي و أحط النقطة التاني و واحد سانتي و أحط
+
+104
+00:07:12,920 --> 00:07:17,140
+النقطة التالتة فكل نقطة بينها و بين البداية و
+
+105
+00:07:17,140 --> 00:07:20,220
+بينها و بين الجانبها واحد سانتي بدي أجيس و أحط
+
+106
+00:07:20,220 --> 00:07:25,990
+النقطةأنا هكذا بكون جهزت الورقة أنه من تحت جسد 8
+
+107
+00:07:25,990 --> 00:07:30,750
+من 10 سانتي و من فوق 5 من 10 سانتي بعدين على خط
+
+108
+00:07:30,750 --> 00:07:36,030
+البداية اللي أنا رسمته تحت بدي أجيس من الحفة نص
+
+109
+00:07:36,030 --> 00:07:40,880
+سانتي و أحط أول واحد أول مكوّنبعدين بدي أجيس بينه
+
+110
+00:07:40,880 --> 00:07:44,260
+وبين التاني واحد سانتي و أحط المكون التاني و واحد
+
+111
+00:07:44,260 --> 00:07:48,480
+سانتي و أحط المكون التاني من على الجدار لأول مكون
+
+112
+00:07:48,480 --> 00:07:52,540
+بجيس بس نص سانتي بس بين المكون و التاني بجيس واحد
+
+113
+00:07:52,540 --> 00:07:56,740
+سانتي هلجيت الورقة صارت عندي جاهزة زيك و مرسوم
+
+114
+00:07:56,740 --> 00:07:59,640
+الخط البداية و الخط النهائية و أين بدي أحط كل مكون
+
+115
+00:07:59,640 --> 00:08:05,640
+بجيب هذا الخليط و بحطعلى كل مكان بكون كتب اسمها
+
+116
+00:08:05,640 --> 00:08:08,860
+مثلا ال اول مكتشر واحد مكتشر اتنين و لو بدي اعمل
+
+117
+00:08:08,860 --> 00:08:13,020
+بس glycine لحال و cysteine لحال و bruline لحال بحط
+
+118
+00:08:13,020 --> 00:08:17,960
+لكل واحدة في مكان من ال cup بتاعها خليت الأول باخد
+
+119
+00:08:17,960 --> 00:08:21,700
+بال capillary tube القنبوبة الشعرية و بحط نقطة على
+
+120
+00:08:21,700 --> 00:08:25,220
+مكانها الأول التانية بحط بال capillary tube و بحط
+
+121
+00:08:25,220 --> 00:08:29,760
+نقطةهلجيت خلصة الورقة ال stationary phase جاهزة
+
+122
+00:08:29,760 --> 00:08:34,020
+ومحطوط عليها الخليط بجيب ال beaker اللي يحتوي على
+
+123
+00:08:34,020 --> 00:08:40,240
+ال mobile phaseوبحطها بغمرها يعني الورقة بغمرها في
+
+124
+00:08:40,240 --> 00:08:43,840
+ال mobile phase ال stationary phase اللي هي الورقة
+
+125
+00:08:43,840 --> 00:08:48,660
+بغمرها في ال mobile phase وبسيبها لحد ما يرتفع
+
+126
+00:08:48,660 --> 00:08:52,820
+السائل في ال mobile phase اللي هو ال mobile phase
+
+127
+00:08:52,820 --> 00:08:57,980
+هذا السائل بدي يرتفع عبر ال stationary phaseبيرتفع
+
+128
+00:08:57,980 --> 00:09:01,840
+و بيوصل لما يجرب يوصل من خط النهاية بقى أشيل
+
+129
+00:09:01,840 --> 00:09:05,940
+الورقة من ال mobile face بشيلها من ال beacon و
+
+130
+00:09:05,940 --> 00:09:11,700
+بطلحها بسيبها جف في الفرن في ال oven بعد ما جف
+
+131
+00:09:11,700 --> 00:09:16,860
+بطلحها مش هنشوف النقاط مش هنشوف كيف فصل ليش لأنه
+
+132
+00:09:16,860 --> 00:09:20,440
+أصلا بتكون colorless المواد اللي فصلت أحمد عمانية
+
+133
+00:09:20,440 --> 00:09:24,220
+colorlessماذا أفعل؟ بجيب الـ developing بعدما جف
+
+134
+00:09:24,220 --> 00:09:27,260
+بجيب الـ developing solution اللي هو المحلول اللي
+
+135
+00:09:27,260 --> 00:09:30,960
+بيظهر اللون بقرشه عبارة عن بخاخ بقرش منه على
+
+136
+00:09:30,960 --> 00:09:36,380
+الورقة و بحطها في الفرن الجافبعد ما اتجف ويزود
+
+137
+00:09:36,380 --> 00:09:39,420
+التفاعل هذا الفرن بين الـ Nenhydrin و المركّب
+
+138
+00:09:39,420 --> 00:09:43,520
+الحمض الأميني أطلّحها من الفرن بشوف أنه فصل عندي
+
+139
+00:09:43,520 --> 00:09:48,520
+على شكل Spot على شكل نقاط ملونة كل نقطة في مكان
+
+140
+00:09:48,520 --> 00:09:53,340
+بحسب لكل نقطة ال retention factor بدي أجيز المسافة
+
+141
+00:09:53,340 --> 00:09:56,620
+اللي مشيتها النقطة من خط البداية لأين مشيت على
+
+142
+00:09:56,620 --> 00:10:00,920
+النقطة اللي مشي على المسافة اللي مشيها المحلول من
+
+143
+00:10:00,920 --> 00:10:03,480
+البداية للنهاية اللي مشيها ال mobile phase من
+
+144
+00:10:03,480 --> 00:10:08,240
+البداية للنهايةبصير عندي هيك معروف لكل نقطة ال
+
+145
+00:10:08,240 --> 00:10:11,700
+retention factor برجع للجدول، هي في عندنا جدول
+
+146
+00:10:11,700 --> 00:10:15,280
+بالاخر بيوضح أنه كل حمض أميني جديش إيه ال
+
+147
+00:10:15,280 --> 00:10:18,620
+retention factor أنا بكون حسبت ال retention factor
+
+148
+00:10:18,620 --> 00:10:24,540
+على فرض طلع معاهي 55% باجي على هذا الجدول 55% من
+
+149
+00:10:24,540 --> 00:10:27,460
+هو الحمض الأميني ال triptofan إذا هذا هو ال
+
+150
+00:10:27,460 --> 00:10:32,000
+triptofan مثلا طلعت معاهي 5 من 100 من هذا بيطلع ال
+
+151
+00:10:32,000 --> 00:10:39,370
+arginine وهكذاهذه التقنية ككل بيكر انها يحتوي على
+
+152
+00:10:39,370 --> 00:10:42,610
+ال mobile phase ال solvent تحت وهذا العمود هو
+
+153
+00:10:42,610 --> 00:10:45,910
+عبارة عن ال stationary phase بعد ما احط عليها
+
+154
+00:10:45,910 --> 00:10:51,570
+الخليط بغمرها في داخل ال mobile phase و بيبدأ يمر
+
+155
+00:10:51,570 --> 00:10:54,770
+الساقل عبرها لما يصل خط النهائي بطلحها
+
+156
+00:10:55,980 --> 00:10:59,060
+Determination of amino acid using thin layer
+
+157
+00:10:59,060 --> 00:11:03,200
+chromatography انا بدي استخدم a thin layer
+
+158
+00:11:03,200 --> 00:11:06,940
+chromatography في هذا المعمل عشان افصل الأحماض
+
+159
+00:11:06,940 --> 00:11:10,480
+الأمنية خليط من أحماض أمنية طيب حكينا الأحماض
+
+160
+00:11:10,480 --> 00:11:14,780
+الأمنية colorless كيف بديك تتعرفي عليها بيحكي اللي
+
+161
+00:11:14,780 --> 00:11:19,160
+بنتعرف عليها باستخدام حاجات عدة مكونات Developing
+
+162
+00:11:19,160 --> 00:11:23,910
+solution سميناها Developing solution هايأبارعاً
+
+163
+00:11:23,910 --> 00:11:27,790
+مادة بتلون الأشياء فبقدر أعرف بتلونها و بتخليها
+
+164
+00:11:27,790 --> 00:11:31,990
+ملونة و بعرف ما كانت اش ممكن استخدم اشهر اشي ممكن
+
+165
+00:11:31,990 --> 00:11:35,870
+استخدم مع ال amino acid هي ال Nihidrin Spray برش
+
+166
+00:11:35,870 --> 00:11:42,190
+من نهيدرين بختار بيستخدم ك visualizing agent عمل
+
+167
+00:11:42,190 --> 00:11:46,450
+مكشف بقدر اكتشف منه الأحمد الأمينية انه بلونها
+
+168
+00:11:46,450 --> 00:11:50,350
+حكينا اللي هو مبدأ عمل ال Nihidrin انه بيتكون عند
+
+169
+00:11:50,350 --> 00:11:56,460
+رومانس كمبليكس لونه بيربو ممكن استخدم iodine vapor
+
+170
+00:11:56,460 --> 00:12:00,600
+as indicator انه باروش من ال iodine و هو بيستخدم ك
+
+171
+00:12:00,600 --> 00:12:04,540
+indicator برضه بيتفاعل مع الحماد الأمنى و ممكن
+
+172
+00:12:04,540 --> 00:12:08,780
+استخدم fluorescence indicator بستخدم صفقة مشعة
+
+173
+00:12:10,950 --> 00:12:15,030
+بترتبط في داخل الأحمد الأمينية وبتعطيني إشعاع
+
+174
+00:12:15,030 --> 00:12:18,990
+باستخدم ultraviolet lamp باللامدة المشعة وين
+
+175
+00:12:18,990 --> 00:12:21,790
+المكان اللي بيشع فيه اللي بيظهر فيه إشعاع إذا هاد
+
+176
+00:12:21,790 --> 00:12:25,870
+عبارة عن حمد أميني فاصل في هذه المنطقة بحسبله
+
+177
+00:12:25,870 --> 00:12:30,130
+retention factor وين ما يعطيني إشعاع تاني مشعق
+
+178
+00:12:30,130 --> 00:12:34,130
+ظهرت إشعاع تحت ال ultraviolet lamp بيكون برضه نقطة
+
+179
+00:12:34,130 --> 00:12:40,650
+حمد أميني آخرهنا عندنا صورة عندنا مثال حط اعطيني
+
+180
+00:12:40,650 --> 00:12:44,530
+على اليمين عندك نقطة الزرقاء خط البداية من نقطة
+
+181
+00:12:44,530 --> 00:12:50,770
+الزرقاء اللي تحت تمام؟ هي لما انفصلت مشيت مسافة من
+
+182
+00:12:50,770 --> 00:12:53,630
+خط البداية من عند وين كانت لو وين وصلت هي اتنين
+
+183
+00:12:53,630 --> 00:12:58,960
+وواحد صاندرالموبايل فاس جدش مشي من البداية للنهاية
+
+184
+00:12:58,960 --> 00:13:03,140
+مشي بيحكي ل 2.8 صندوق كيف بده أحسب ال retention
+
+185
+00:13:03,140 --> 00:13:09,680
+factor؟ بجسم 2 و 1 على 2 و 8 بيطلع 75 من 100 هي ال
+
+186
+00:13:09,680 --> 00:13:13,760
+retention factor لهذه المادة برجع للجدول بشوف من
+
+187
+00:13:13,760 --> 00:13:20,240
+هذه ال 75% من هي المادة و بطلع اسمهاهنا نفس الشيء
+
+188
+00:13:20,240 --> 00:13:23,940
+كيف بدأ أحسب ال retention factor اللي هنا بمثلها
+
+189
+00:13:23,940 --> 00:13:28,260
+بي على ا برموز بي على ا اللي هي المسافة اللي
+
+190
+00:13:28,260 --> 00:13:31,880
+قطعتها المادة من البداية لون وصلت على المسافة اللي
+
+191
+00:13:31,880 --> 00:13:36,280
+قطعها ال solvent من البداية للنهاية هنا جدول فيه
+
+192
+00:13:36,280 --> 00:13:40,340
+بعض الأحمد الأمينية و retention factor لكل حمض
+
+193
+00:13:40,340 --> 00:13:47,560
+أميني وهذا هو كل المعمل لهذه التجربة أتمنى تكونوا
+
+194
+00:13:47,560 --> 00:13:48,180
+فهمتها
+
diff --git a/PL9fwy3NUQKwZ1Q7zOg1ObgufPMjRI3WY1/6BIvAPIvRuM.srt b/PL9fwy3NUQKwZ1Q7zOg1ObgufPMjRI3WY1/6BIvAPIvRuM.srt
new file mode 100644
index 0000000000000000000000000000000000000000..a881a38fc94da9fb1e6c82ebf297aa6a98d14a87
--- /dev/null
+++ b/PL9fwy3NUQKwZ1Q7zOg1ObgufPMjRI3WY1/6BIvAPIvRuM.srt
@@ -0,0 +1,863 @@
+1
+00:00:00,000 --> 00:00:04,520
+هنا عندنا جدول بيقارن بين أنواع الـOxidizing agent
+
+2
+00:00:04,520 --> 00:00:08,460
+المستخدم نقارن بين الـBendix solution و الـ Fehling solution
+
+3
+00:00:08,460 --> 00:00:12,180
+و الـTollens reagent أولا يجي من ناحية
+
+4
+00:00:12,180 --> 00:00:16,640
+المكونات الـBendix بيتكون من كبريتات في ألكالاين
+
+5
+00:00:16,640 --> 00:00:21,060
+كوبريك دوّب في ألكالاين كوبرتات  الـFehling بيتكون من
+
+6
+00:00:21,060 --> 00:00:26,100
+كبريتات في ألكالاين تارترات الـTolens بيتكون من
+
+7
+00:00:26,100 --> 00:00:30,530
+silver nitrate في أكي واساسمان  طيب color of
+
+8
+00:00:30,530 --> 00:00:34,130
+solution إيش لون الـReagent بيكون؟ لون الـReagent 
+
+9
+00:00:34,130 --> 00:00:37,610
+في حالة الـBendix بيكون ديپ بلو وفي حالة الـ
+
+10
+00:00:37,610 --> 00:00:41,290
+Fehling برضه ديپ بلو بيكون لونهم أزرق حكينا بسبب
+
+11
+00:00:41,290 --> 00:00:44,490
+وجود الكوبر هو اللي بيعطي الـReagent اللون الأزرق
+
+12
+00:00:44,490 --> 00:00:49,390
+الـTulens بيكون colorless مالهوش لون طيب، بعد
+
+13
+00:00:49,390 --> 00:00:52,390
+التفاعل color after reaction with reducing sugar
+
+14
+00:00:52,390 --> 00:00:57,730
+بعد التفاعل الأكسدة والاختزال، إيش هيصير اللون؟
+
+15
+00:00:57,730 --> 00:01:01,610
+الـBendix والـFehling بيتحول لونهم إلى brick-red
+
+16
+00:01:01,610 --> 00:01:05,570
+بريسيبيتيت بتاعه اللي هو راسب لونه أحمر، طوبي،
+
+17
+00:01:05,570 --> 00:01:10,030
+ليتكون الكوبر أكسيد أما الـTolens، بيتحول إلى لون
+
+18
+00:01:10,030 --> 00:01:13,710
+silver mirror form اللي هو بيكون لون فضي وعمل زي
+
+19
+00:01:13,710 --> 00:01:19,690
+المرايا بسبب تكون الـAg الفضه الـ Species being
+
+20
+00:01:19,690 --> 00:01:23,590
+reduced مين هي المكونات اللي هيصير لها اختزال؟
+
+21
+00:01:23,590 --> 00:01:28,550
+السكر هو اللي هيختزل غيره، فالـ Oxidizing agent هو
+
+22
+00:01:28,550 --> 00:01:32,030
+اللي هيصير له اختزال، مين اللي هيصير له اختزال هو
+
+23
+00:01:32,030 --> 00:01:35,310
+الـ Oxidizing agent في حالة الـ Bendect solution
+
+24
+00:01:35,310 --> 00:01:39,570
+الكوبر هو اللي هيصير له اختزال هيتحول من Cupric Ion
+
+25
+00:01:39,570 --> 00:01:43,190
+إلى Cuprous Ion ونفس الشيء بالنسبة للـ Fehling هو
+
+26
+00:01:43,190 --> 00:01:47,170
+الـ Cupric Ion هيتحول إلى Cuprous Ion في حالة الـ
+
+27
+00:01:47,170 --> 00:01:51,710
+Tollens هي الفضة هي اللي هتختزل من Ag+ إلى Ag
+
+28
+00:01:52,660 --> 00:01:56,600
+طيب، species being oxidized مين اللي هيصيرله
+
+29
+00:01:56,600 --> 00:02:00,520
+أكسدة؟ السكر هو اللي هيصيرله أكسدة في كل الحالات
+
+30
+00:02:00,520 --> 00:02:04,300
+لأن هو بيختزل غيره و بيصيرله أكسدة فال reducing
+
+31
+00:02:04,300 --> 00:02:08,420
+sugar oxidized too حسب نوعه، هيتأكسد إما
+
+32
+00:02:08,420 --> 00:02:13,880
+لكربوكسيلات أو هيدروكسيل كربوكسيلات في الثلاث حالات
+
+33
+00:02:13,880 --> 00:02:21,050
+اللي بيصيرله أكسدة هو السكر هنا لصورة يوتيوب نوعتتش
+
+34
+00:02:21,050 --> 00:02:25,950
+باستخدام tool insert agent وبعد التفاعل كيف بيكون
+
+35
+00:02:25,950 --> 00:02:29,190
+اللون حاجة أنها بيكون silver mirror form اللي هو
+
+36
+00:02:29,190 --> 00:02:33,470
+لون فضي بس شكله زي المرايا هيك لامع فهذا هو نوعتتش
+
+37
+00:02:33,470 --> 00:02:38,990
+كيف هيك شكله فحوصاتنا لليوم هي فحوصات qualitative
+
+38
+00:02:38,990 --> 00:02:43,630
+test للكشف عن وجود السكريات تمام، هي فحوصات
+
+39
+00:02:43,630 --> 00:02:47,950
+qualitative للكشف عن وجود الـCarbohydrate أول فحص
+
+40
+00:02:47,950 --> 00:02:51,830
+نتعرف عليه هو الـBindect Test الـBindect، الـ
+
+41
+00:02:51,830 --> 00:02:54,790
+objective منه أو هو ولا إيش هذا الفحص to detect
+
+42
+00:02:54,790 --> 00:02:57,650
+the presence of reducing sugar distinguished
+
+43
+00:02:57,650 --> 00:03:01,470
+between reducing and non-reducing sugar وفحص خاص
+
+44
+00:03:01,470 --> 00:03:06,030
+للكشف عن الـreducing sugar عن السكريات المختزلة
+
+45
+00:03:06,920 --> 00:03:10,640
+فبيميز من الـreducing و الـnon-reducing شغل مثلا
+
+46
+00:03:10,640 --> 00:03:13,360
+أعطيتك سكر وحكيتلك هل هذا reducing او non
+
+47
+00:03:13,360 --> 00:03:16,820
+-reducing كيف بدك تقرر فيه؟ بدك تشتغل فحص الـ
+
+48
+00:03:16,820 --> 00:03:20,480
+Benedict طيب على أي مبدأ بيشتغل الـprinciple
+
+49
+00:03:20,480 --> 00:03:24,260
+بيشتغل على مبدأ أن هذا الـReagent يحتوي على كوبر
+
+50
+00:03:24,260 --> 00:03:29,200
+كبريتات على كوبر كبريتات الكوبر هذا الموجود في كوبر
+
+51
+00:03:29,200 --> 00:03:34,920
+كبريتات يعمل كـOxidizing agent بيروح بيؤكسد السكر
+
+52
+00:03:35,220 --> 00:03:42,620
+والسكر بيشتغل كـReducing agent بيعمل اختزال للكوبر
+
+53
+00:03:42,620 --> 00:03:47,340
+فالـPrinciple كيف هتكون على شكل المعادلات زي ما
+
+54
+00:03:47,340 --> 00:03:50,920
+أخدنا في المعمل الأول لو بدنا نعملها أنه عندي سكر
+
+55
+00:03:50,920 --> 00:03:54,120
+على فرض أنه ألدهيد هو إما هيكون ألدهيد أو
+
+56
+00:03:54,120 --> 00:03:57,720
+كيتون نعمل معادلة للألدهيد لحال ومعادلة
+
+57
+00:03:57,720 --> 00:04:03,440
+للكيتون لحال هو ألدهيد زائد الكوبر كايون اللي موجود
+
+58
+00:04:03,440 --> 00:04:08,420
+في الكوبر كبريتات في وجود alkaline media alkaline pH
+
+59
+00:04:08,420 --> 00:04:12,740
+بيعطينا الناتج الألدهيد هيصير له oxidation
+
+60
+00:04:12,740 --> 00:04:17,720
+تحول إلى كربوكسيلات طب والكوبر كايون هيصير له
+
+61
+00:04:17,720 --> 00:04:22,640
+reduction إلى كوبر اكسيد بيرسب على شكل كوبر
+
+62
+00:04:22,640 --> 00:04:28,680
+أكسيد لونه brick red أحمر طوبي فإذا الناتج أعطاني
+
+63
+00:04:28,680 --> 00:04:32,380
+لون اتحول اللون إلى brick red إذا هذا reducing
+
+64
+00:04:32,380 --> 00:04:37,480
+sugar إذا ما أعطانيش إذا non-reducing sugar لو بدك
+
+65
+00:04:37,480 --> 00:04:42,410
+تعمل معادلة ثانية للكيتون، الكيتون زائد
+
+66
+00:04:42,410 --> 00:04:46,810
+كوبر كبريتات اللي هو الـCupric Ion في وجود alkaline
+
+67
+00:04:46,810 --> 00:04:50,510
+media الكيتون بدي يصير له oxidation بس بدي يتحول
+
+68
+00:04:50,510 --> 00:04:54,910
+إلى هيدروكسيل كربوكسيلات طيب، والـCupric Ion
+
+69
+00:04:54,910 --> 00:04:59,070
+بيرسب، بيصير له reduction، بيرسب على شكل Cuprous
+
+70
+00:04:59,070 --> 00:05:05,830
+Oxide لونه بريك ريد بريسيبيتيت بيحكيلي the color of
+
+71
+00:05:05,830 --> 00:05:10,130
+بريسيبيتيت بتعتمد شدة اللون بتعتمد على تركيز
+
+72
+00:05:10,130 --> 00:05:16,610
+reducing sugar كده ش في كمية من هذا السكر خطوات
+
+73
+00:05:16,610 --> 00:05:20,650
+إجراء التجربة هاي أني بحط واحد من من السكر اللي
+
+74
+00:05:20,650 --> 00:05:23,750
+موجود عندي مثلا عندي عدة سكريات سكر جلوكوز،
+
+75
+00:05:23,750 --> 00:05:27,810
+فركتوز، سكروز، لاكتوز، ملتوز، ودول بدكشف عنهم،
+
+76
+00:05:27,810 --> 00:05:31,310
+كل واحد بشتغلوا بتيوم بحط واحد من من هذا السكر
+
+77
+00:05:31,310 --> 00:05:34,930
+واضيف عليه واحد من من الـBindex Reagent من
+
+78
+00:05:34,930 --> 00:05:39,310
+الـReagent وبحطهم في الـwater bath لمدة خمس دقائق
+
+79
+00:05:39,880 --> 00:05:43,040
+بعدين بطلع إذا اتحول اللون إلى brick red إلى لون
+
+80
+00:05:43,040 --> 00:05:47,240
+أحمر إذا هذا reducing sugar إذا ما تغيرش اللون
+
+81
+00:05:47,240 --> 00:05:52,020
+إذا هو non-reducing sugar أو إنه طلع لون ثاني غير
+
+82
+00:05:52,020 --> 00:05:57,840
+الـbrick red هو non-reducing sugar الفحص الثاني
+
+83
+00:05:57,840 --> 00:06:02,760
+اسمه Barfoed Test Barfoed Test هذا إيش الـ
+
+84
+00:06:02,760 --> 00:06:06,080
+objective منه؟ to distinguish between mono and
+
+85
+00:06:06,080 --> 00:06:09,960
+disaccharide بيفرق من السكريات الأحادية والثنائية
+
+86
+00:06:09,960 --> 00:06:12,800
+يعني لو عندي سكر مش عارف هل هو mono ولا
+
+87
+00:06:12,800 --> 00:06:16,380
+disaccharide بستخدم فحص الـBarfoed Test عشان
+
+88
+00:06:16,380 --> 00:06:20,060
+أفرقه هل هو mono ولا disaccharide كيف الـprinciple
+
+89
+00:06:20,060 --> 00:06:25,320
+طريقة شغله الـprinciple إنه الـParfoed reagent
+
+90
+00:06:25,320 --> 00:06:30,750
+اللي أنا بستخدمه بيتكون main coverage إيه طبعا عند
+
+91
+00:06:30,750 --> 00:06:34,850
+الـReagent بيحتوي على Cupric Ion فأنا بحط السكر،
+
+92
+00:06:34,850 --> 00:06:38,370
+السكر هذا نوعيا هيكون ألدهيد يا كيتو مثلا
+
+93
+00:06:38,370 --> 00:06:41,890
+ألدهيد، نيجي نكتب معادلة ألدهيد الـAldehyde
+
+94
+00:06:41,890 --> 00:06:44,730
+زائد الـCupric Ion اللي موجود في الـReagent
+
+95
+00:06:44,730 --> 00:06:48,470
+بيتحول، بيطلع عندي الناتج الـAldehyde بتحول
+
+96
+00:06:48,470 --> 00:06:54,940
+لكربوكسيلات وCuprous Oxide أما إذا كان كيتون كيتون
+
+97
+00:06:54,940 --> 00:06:57,400
+باضيف عليه الـReagent اللي هو الـCupric Ion
+
+98
+00:06:57,400 --> 00:07:02,040
+بتحول الكيتون إلى Hydroxyl Carboxylate والـCupric
+
+99
+00:07:02,040 --> 00:07:06,900
+Ion بتحول إلى Cuprous Oxide اللون في الآخر في
+
+100
+00:07:06,900 --> 00:07:11,280
+المعادلتين اللون بيكون brick red إذا اتحول إلى لون
+
+101
+00:07:11,280 --> 00:07:16,440
+brick red بيكون هذا monosaccharide بيحكي في الـ
+
+102
+00:07:16,440 --> 00:07:18,900
+Parfoed reagent react with monosaccharide to
+
+103
+00:07:18,900 --> 00:07:21,940
+produce cuprous oxide faster rate than
+
+104
+00:07:21,940 --> 00:07:25,120
+disaccharide الـParfoed reagent بيتفاعل مع
+
+105
+00:07:25,120 --> 00:07:27,160
+الإتنين، مع الـmonosaccharide ومع الـ
+
+106
+00:07:27,160 --> 00:07:30,830
+disaccharide بيعطيني لون ولكن تفاعله مع الـ
+
+107
+00:07:30,830 --> 00:07:34,150
+monosaccharide أسرع من تفاعله مع الـdisaccharide
+
+108
+00:07:34,150 --> 00:07:38,590
+فاحنا لما نشتغل نحطه وقت معين لواجهة هذا بيسمح
+
+109
+00:07:38,590 --> 00:07:42,650
+بتفاعل فقط الـmonosaccharide لو تأخرنا عليه ممكن
+
+110
+00:07:42,650 --> 00:07:46,010
+الـdisaccharide يتفاعل كمان فاحنا بدنا نحطه بس
+
+111
+00:07:46,010 --> 00:07:50,270
+لواجهة تفاعل الـmonosaccharide وبعد هيك بطلع إذا
+
+112
+00:07:50,270 --> 00:07:55,360
+تغير اللون يعني هذا monosaccharide الـPrinciple
+
+113
+00:07:55,360 --> 00:08:00,580
+للبارفويدز زي الـبين ديكتس ولكن بدون وجود وسط
+
+114
+00:08:00,580 --> 00:08:07,300
+قاعد، لا تحتاج إلى وجود وسط قاعدي كيف طريقة العمل؟
+
+115
+00:08:07,300 --> 00:08:10,880
+أحط واحد مل من السكر اللي موجود عندي على واحد مل
+
+116
+00:08:10,880 --> 00:08:15,040
+من الـReagent ثم بحطه في الـwater bath لمدة عشر دقائق
+
+117
+00:08:15,040 --> 00:08:18,060
+بطلع من الـwater bath اللي تكون عندي red
+
+118
+00:08:18,060 --> 00:08:21,540
+precipitate اللون الأحمر، الراسب اللون الأحمر، إذا
+
+119
+00:08:21,540 --> 00:08:25,600
+ده mono-saccharide إذا ما تكونش، إذا هو di
+
+120
+00:08:25,600 --> 00:08:31,990
+-saccharide أو سكر آخر فحص الثالث هو فحص الـBial's أو
+
+121
+00:08:31,990 --> 00:08:37,030
+اسمه ثاني orcinol test Bial's or orcinol هذا خاص
+
+122
+00:08:37,030 --> 00:08:40,550
+للكشف عن الـPentose objective for the detection of
+
+123
+00:08:40,550 --> 00:08:43,710
+pentose it distinguishes between pentose and
+
+124
+00:08:43,710 --> 00:08:48,750
+hexose بيفرق بين الخماسي والسداسي هو للكشف عن الـ
+
+125
+00:08:48,750 --> 00:08:53,570
+Pentose عن الخماسي فبيفرق بين الخماسي والسداسي كيف
+
+126
+00:08:53,570 --> 00:08:58,010
+الـPrinciple أنا بستخدم Bial's reagent Bial's reagent
+
+127
+00:08:58,340 --> 00:09:01,380
+اللي هو شو مكوناته هذا الـBial's Region واحد من
+
+128
+00:09:01,380 --> 00:09:06,000
+عشرة في المية أورسينول Concentrated HCl واحد من
+
+129
+00:09:06,000 --> 00:09:11,930
+عشرة في المية فرّيك كلوريد FeCl3 H2O إن هذا الـ
+
+130
+00:09:11,930 --> 00:09:16,150
+Concentrated HCl بيعمل الـdehydration للسُكّر
+
+131
+00:09:16,150 --> 00:09:20,230
+قلت الـBial's بيفرق بين الـPentose و الـHexose
+
+132
+00:09:20,230 --> 00:09:24,010
+كيف بدي أتفاعل الـPrinciple بحط Bial's بحط السُكّر
+
+133
+00:09:24,010 --> 00:09:28,310
+أنا مثلا Pentose و بحط عليه الـBial's reagent
+
+134
+00:09:28,310 --> 00:09:33,130
+المكونات الثلاث طبعا كلهم مخلطين بيتحول السُكّر
+
+135
+00:09:33,130 --> 00:09:37,990
+إلى حاجة اسمها Furfural فيرفورال و أورسينول
+
+136
+00:09:37,990 --> 00:09:42,590
+اللي ضال عندنا بتحول السكر إلى فيرفورال فيرفورال
+
+137
+00:09:42,590 --> 00:09:47,650
+مع أورسينول في وجود فرّيك ايون من الفرّيك كلوريد جاي
+
+138
+00:09:47,650 --> 00:09:55,030
+بتكون عندي لون blue to green لون أخضر مزرق أو
+
+139
+00:09:55,030 --> 00:09:58,910
+precipitate على شكل هذا اللون اللي هو أخضر مزرق
+
+140
+00:09:58,910 --> 00:10:02,610
+بيطلع عندي إذا طلع هذا اللون إذا الموجود بنتوز
+
+141
+00:10:02,610 --> 00:10:06,490
+بيحكي لي إذا طولت عليه شويه يصير اللون براون بتكون
+
+142
+00:10:06,490 --> 00:10:10,370
+لون براون precipitate لون راسب لونه بني بيكون
+
+143
+00:10:10,370 --> 00:10:16,240
+الموجود hexose فلو بدك تلخص معادلة الـBial's على
+
+144
+00:10:16,240 --> 00:10:19,720
+شكل معادلة، تلخص الـPrinciple على شكل معادلة، أنه
+
+145
+00:10:19,720 --> 00:10:21,740
+الـPentose، هذا الفحص كان للـPentose، الـ
+
+146
+00:10:21,740 --> 00:10:26,240
+Pentose في وجود HCl، أول حاجة بتشتغل من الريجنت
+
+147
+00:10:26,240 --> 00:10:30,200
+اللي هو الـHCl، بيعمل dehydration للسكر، فبينتج
+
+148
+00:10:30,200 --> 00:10:35,620
+عنه، بيتحول إلى Furfural زائد Orcinol زائد Ferric
+
+149
+00:10:35,620 --> 00:10:40,740
+Chloride إذا أنا عند الـPentose في وجود HCl
+
+150
+00:10:40,740 --> 00:10:46,620
+بيعطيني Furfural زائد Orcinol زائد Ferric Chloride هدول
+
+151
+00:10:46,620 --> 00:10:50,100
+الثلاث مع بعض هالـثلاث بيتفعلوا بيعطوني Complex
+
+152
+00:10:50,100 --> 00:10:57,060
+لونه blue to green Complex لونه blue to green كيف
+
+153
+00:10:57,060 --> 00:11:02,320
+خطوات العمل بحط واحد مل من السكر على واحد ونصف مل
+
+154
+00:11:02,320 --> 00:11:07,320
+من الـBial's reagent وبعملهم خلط mix واحد مل من
+
+155
+00:11:07,320 --> 00:11:11,020
+السكر على واحد ونصف مل من الـReagent بعد هيك
+
+156
+00:11:11,020 --> 00:11:14,180
+بسخنها على اللهب مش في الـwater bath هي على اللهب
+
+157
+00:11:14,180 --> 00:11:18,420
+بسخنها لحد ما تغلي بعد ما تغلي بحطها بالراكد تبرد
+
+158
+00:11:18,420 --> 00:11:21,960
+إذا اتحول اللون إلى blue to green is positive إذا
+
+159
+00:11:21,960 --> 00:11:25,540
+السكر اللي موجود عندي بنتوز إذا ما تحولش إذا
+
+160
+00:11:25,540 --> 00:11:31,050
+negative الفحص اللي بعده اسمه Seliwanoff's أو Resorcinol Test Seliwanoff's أو Resorcinol Test إيش
+
+161
+00:11:31,050 --> 00:11:35,790
+الهدف من هذا الفحص for detection of ketoses يكشف
+
+162
+00:11:35,790 --> 00:11:39,110
+عن الـketoses السكر اللي يحتوي على كيتون اجرام
+
+163
+00:11:39,110 --> 00:11:42,930
+الـPrinciple Seliwanoff's Test to distinguish between
+
+164
+00:11:44,450 --> 00:11:48,850
+hexose with a ketone group and hexose with
+
+165
+00:11:48,850 --> 00:11:52,290
+aldehyde group هو الكشف بيفرق بين اللي عنده
+
+166
+00:11:52,290 --> 00:11:55,610
+ألدهيد عن اللي عنده كيتون ويكون hexose يكون
+
+167
+00:11:55,610 --> 00:11:59,810
+سداسي هذا الفحص لو اشتغلتي مع كيتوز، مع كيتوز اللي
+
+168
+00:11:59,810 --> 00:12:06,070
+عنده كيتون جروب، بيعطيكي لون Red، هيعطيكي لون أحمر
+
+169
+00:12:06,070 --> 00:12:10,070
+وبيتكون بسرعة، it's formed rapidly، إذا اتكون لون
+
+170
+00:12:10,070 --> 00:12:13,390
+أحمر بسرعة، إذا اللي موجود كيتون، طيب الألدهوز
+
+171
+00:12:13,390 --> 00:12:17,590
+بتعطيني light pink color و بتاخد واجهة أطول، واجهة
+
+172
+00:12:17,590 --> 00:12:21,410
+أطول، longer time إذا اتكون عندي اللون البنك وبوجهة
+
+173
+00:12:21,410 --> 00:12:26,510
+أطول لما يتكون إذا اللي موجود عندي الـAldose
+
+174
+00:12:26,510 --> 00:12:29,790
+الـKetohexose يعني هو سداسي وعنده ketone group زي
+
+201
+00:14:27,470 --> 00:14:32,610
+بتعملها على شكل معادلة بحكي كيتوهيكسوز في وجود HCl
+
+202
+00:14:32,610 --> 00:14:37,170
+بيعطيني Hydroxyl Methyl Furfural بيتفاعل مع
+
+203
+00:14:37,170 --> 00:14:43,450
+الرسورسينول بيعطيني Colored Complex كيف خطوات إجراء
+
+204
+00:14:43,450 --> 00:14:47,970
+التجربة؟ بحط تلاتة مل من العينة على drop واحدة من
+
+205
+00:14:47,970 --> 00:14:52,010
+السكر بعدين بحطه في الـ water bath لمدة تلاتة مل 
+
+206
+00:14:52,010 --> 00:14:55,670
+بعدين بلاحظ إذا اتكون عندي اللون الأحمر إذا هذا 
+
+207
+00:14:55,670 --> 00:15:00,470
+positive وهذا عبارة عن كيتوز إذا ما اتكونش إذا 
+
+208
+00:15:00,470 --> 00:15:06,030
+negative آخر فحص هو الـ  Bennedict's Test فحص الـ
+
+209
+00:15:06,030 --> 00:15:09,990
+Bennedict's  الـ objective منه يستخدم للكشف عن الـ
+
+210
+00:15:09,990 --> 00:15:13,810
+reducing sugar يعني زيه زي أول فحص اللي هو الـ
+
+211
+00:15:13,810 --> 00:15:18,390
+Benedict's Test للكشف عن الـ reducing sugar زي أول
+
+212
+00:15:18,390 --> 00:15:22,670
+فحص اللي هو الـ Benedict's Test كيف الـ principle أنه
+
+213
+00:15:22,670 --> 00:15:26,470
+عندي Bicric Acid اللي هو اسمه تاني اتنين اربع ستة
+
+214
+00:15:26,470 --> 00:15:30,990
+Trinitrophenol Bicric Acid هو اتنين اربع ستة 
+
+215
+00:15:30,990 --> 00:15:35,680
+Trinitrophenol أو الـ TMP بيجي هذا الـ bicric acid
+
+216
+00:15:35,680 --> 00:15:40,260
+بيتفعل مع الـ Reducing Sugar وبيصير لو واحد فيهم
+
+217
+00:15:40,260 --> 00:15:44,780
+أكسدة وواحد فيهم اختزال وبيتكون عندي الـ Red Color
+
+218
+00:15:44,780 --> 00:15:50,760
+جاي من وين هذا الـ Red Color من عملية اختزال
+
+219
+00:15:50,760 --> 00:15:57,500
+الـ Bicric Acid إلى Sodium Bicaramide الـ Red color زي
+
+220
+00:15:57,500 --> 00:16:00,960
+من حدوث reduction للـ  Bicric Acid لأنه بيصيبله
+
+221
+00:16:00,960 --> 00:16:04,980
+اختزال، بتحول لـ Sodium Bicaramide فلو بدك تكتب
+
+222
+00:16:04,980 --> 00:16:08,400
+معادلة للـ Principle للـ Bicric Acid أن عندي
+
+223
+00:16:08,400 --> 00:16:13,660
+Aldehyde زائد Bicric Acid في وجود أنيونات، في وجود
+
+224
+00:16:13,660 --> 00:16:19,270
+قاعدة الـ Aldehyde بيصير له oxidation بيتأكسد إلى 
+
+225
+00:16:19,270 --> 00:16:22,790
+حاجة اسمها الـ Carboxylate حكينا الـ Bicric Acid
+
+226
+00:16:22,790 --> 00:16:26,390
+بيصير له reduction إلى أيش؟ إلى Sodium Bicaramate
+
+227
+00:16:26,390 --> 00:16:30,010
+Sodium Bicaramate هذا له لون أحمر، طب إذا كيتون مش
+
+228
+00:16:30,010 --> 00:16:34,380
+Aldehyde؟ الكيتون زائد الـ Bicric Acid في وجود
+
+229
+00:16:34,380 --> 00:16:38,280
+أنيونات OH الكيتون إيش هيصير له oxidation إلى
+
+230
+00:16:38,280 --> 00:16:43,020
+هيدروكسلكربوكسيلات والـ Bicric Acid بيصير له
+
+231
+00:16:43,020 --> 00:16:47,800
+reduction إلى صوديوم بكرامات لونه red إذا اتكون
+
+232
+00:16:47,800 --> 00:16:51,820
+اللون الأحمر إذا هو Reducing Sugar إذا ما اتكونش
+
+233
+00:16:51,820 --> 00:16:58,080
+إذا هو Non-reducing Sugar كيف خطوات العمل بحط واحد
+
+234
+00:16:58,080 --> 00:17:03,750
+مل من السكر على 1 مل من الـ Bicric Acid على نص مل
+
+235
+00:17:03,750 --> 00:17:07,230
+من الـ NaOH من القاعدة و بعدين بحطه في الـ
+
+236
+00:17:07,230 --> 00:17:12,030
+Water Bath لمدة 5 دقائق، لمدة 5 دقائق إذا ظهر بعد
+
+237
+00:17:12,030 --> 00:17:15,470
+ما طلع من الـ Water Bath كان اللون Red إذا هو
+
+238
+00:17:15,470 --> 00:17:20,310
+عبارة عن Reducing Sugar إذا اللون ما اتكونش اللون
+
+239
+00:17:20,310 --> 00:17:25,050
+الأحمر إذا هو Non-Reducing Sugar بهيك بنكون خلصنا
+
+240
+00:17:25,050 --> 00:17:29,980
+تجارب الفحص و الكشف عن السكريات مطلوب منكم تكتبولي
+
+241
+00:17:29,980 --> 00:17:34,960
+الـ principle لكل فحص على شكل معادلة زي ما عملنا في 
+
+242
+00:17:34,960 --> 00:17:38,420
+فحص qualitative test of amino acid 
diff --git a/PL9fwy3NUQKwZ1Q7zOg1ObgufPMjRI3WY1/6BIvAPIvRuM_postprocess.srt b/PL9fwy3NUQKwZ1Q7zOg1ObgufPMjRI3WY1/6BIvAPIvRuM_postprocess.srt
new file mode 100644
index 0000000000000000000000000000000000000000..803fb077363dec2c6365320a511f86a1b3f98f0a
--- /dev/null
+++ b/PL9fwy3NUQKwZ1Q7zOg1ObgufPMjRI3WY1/6BIvAPIvRuM_postprocess.srt
@@ -0,0 +1,968 @@
+1
+00:00:00,000 --> 00:00:04,520
+هنا عندنا جدول بيقارن بين أنواع الـOxidizing agent
+
+2
+00:00:04,520 --> 00:00:08,460
+المستخدم نقارن بين الـBendix solution و الـFilling
+
+3
+00:00:08,460 --> 00:00:12,180
+solution و الـTolens reagent أولا يجي من ناحية
+
+4
+00:00:12,180 --> 00:00:16,640
+المكونات الـBendix بيتكون من كبر صلفات في ألكالاين
+
+5
+00:00:16,640 --> 00:00:21,060
+زيتريا دوّب في ألكالاين زيترا الفilling بيتكون من
+
+6
+00:00:21,060 --> 00:00:26,100
+كبر صلفات في ألكالاين ترتريا الـTolens بيتكون من
+
+7
+00:00:26,100 --> 00:00:30,530
+silver nitrate إن أكي و أسامانةطيب color of
+
+8
+00:00:30,530 --> 00:00:34,130
+solution إيش لون ال reagent بيكون؟ لون ال reagent
+
+9
+00:00:34,130 --> 00:00:37,610
+في حالة ال pendex بيكون دي بي بلو و في حالة ال
+
+10
+00:00:37,610 --> 00:00:41,290
+filling برضه دي بي بلو بيكون لونهم أزرق حكينا بسبب
+
+11
+00:00:41,290 --> 00:00:44,490
+وجود الكبر هو اللي بيعطي ال reagent اللون الأزرق
+
+12
+00:00:44,490 --> 00:00:49,390
+ال tulens بيكون colorless مالهوش لونطيب، بعد
+
+13
+00:00:49,390 --> 00:00:52,390
+التفاعل color after reaction with reducing sugar
+
+14
+00:00:52,390 --> 00:00:57,730
+بعد التفاعل الأكسد والاختزال، إيش هيصير اللون؟
+
+15
+00:00:57,730 --> 00:01:01,610
+الـBendix والـFilling بيتحول لونهم إلى break-red
+
+16
+00:01:01,610 --> 00:01:05,570
+depressive بتاعه اللي هو راسب لونه أحمر، طوبي،
+
+17
+00:01:05,570 --> 00:01:10,030
+ليتكون الكبار أكسان أما الـTolens، بيتحول إلى لون
+
+18
+00:01:10,030 --> 00:01:13,710
+silver mirror form اللي هو بيكون لون فضي وعمل زي
+
+19
+00:01:13,710 --> 00:01:19,690
+المرايا بسبب تكون الـAG الفندقالـ Species being
+
+20
+00:01:19,690 --> 00:01:23,590
+reduced مين هي المكونات اللي هيصير لها اختزال؟
+
+21
+00:01:23,590 --> 00:01:28,550
+السكر هو اللي هيختزل غيره، فالـ Oxidizing agent هو
+
+22
+00:01:28,550 --> 00:01:32,030
+اللي هيصير له اختزال، مين اللي هيصير له اختزال هو
+
+23
+00:01:32,030 --> 00:01:35,310
+الـ Oxidizing agentفي حالة الـ Bendect solution
+
+24
+00:01:35,310 --> 00:01:39,570
+الكبر هو اللي هيصير له اختزال هيتحول من Capric Ion
+
+25
+00:01:39,570 --> 00:01:43,190
+إلى Capric Ion ونفس الشيء بالنسبة للـ Filling هو
+
+26
+00:01:43,190 --> 00:01:47,170
+الـ Capric Ion هيتحول إلى Capric Ion في حالة الـ
+
+27
+00:01:47,170 --> 00:01:51,710
+Tollens هي الفضة هي اللي هتختزل من AG plus إلى AG
+
+28
+00:01:52,660 --> 00:01:56,600
+طيب، species being oxidized مين اللي هيصيرله
+
+29
+00:01:56,600 --> 00:02:00,520
+أكسدة؟ السكر هو اللي هيصيرله أكسدة في كل الحلقة
+
+30
+00:02:00,520 --> 00:02:04,300
+لأن هو بيختزل غيره و بيصيرله أكسدة فال reducing
+
+31
+00:02:04,300 --> 00:02:08,420
+sugar oxidized too حسب نوعه، هيتأكسد إما
+
+32
+00:02:08,420 --> 00:02:13,880
+لكربوكسيلات أو هيدروكسل كربوكسيلات في التلت حالات
+
+33
+00:02:13,880 --> 00:02:21,050
+اللي بيصيرله أكسدة هو السكرهنا لصورة يوتيوب نوعتش
+
+34
+00:02:21,050 --> 00:02:25,950
+باستخدام tool insert agent وبعد التفاعل كيف بيكون
+
+35
+00:02:25,950 --> 00:02:29,190
+اللون حاجة انها بيكون silver mirror phone اللي هو
+
+36
+00:02:29,190 --> 00:02:33,470
+لون فضي بس شكله زي المرايا هيك لامع فهذا هو نوعتش
+
+37
+00:02:33,470 --> 00:02:38,990
+كيف هيك شكلهفحصاتنا لليوم هي فحصات qualitative
+
+38
+00:02:38,990 --> 00:02:43,630
+test للكشف عن وجود السكريات تمام، هي فحصات
+
+39
+00:02:43,630 --> 00:02:47,950
+qualitative للكشف عن وجود الـ Carbohydrate أول فحص
+
+40
+00:02:47,950 --> 00:02:51,830
+نتعرف عليه هو الـ Bindect Test الـ Bindect، الـ
+
+41
+00:02:51,830 --> 00:02:54,790
+objective منه أو هو ولا إيش هذا الفحص to detect
+
+42
+00:02:54,790 --> 00:02:57,650
+the presence of reducing sugar distinguished
+
+43
+00:02:57,650 --> 00:03:01,470
+between reducing and non-reducing sugar وفحص خاص
+
+44
+00:03:01,470 --> 00:03:06,030
+للكشف عن ال reducing sugar عن السكريات المختزلة
+
+45
+00:03:06,920 --> 00:03:10,640
+فبيميز من الـ reducing و الـ non-reducing شغل مثلا
+
+46
+00:03:10,640 --> 00:03:13,360
+اعطيتك سكر و حكيتلك هل هذا reducing او non
+
+47
+00:03:13,360 --> 00:03:16,820
+-reducing كيف بدك تقرا فيه؟ بدك تشتغل فحص الـ
+
+48
+00:03:16,820 --> 00:03:20,480
+vindictive طيب على اي مبدأ بيشتغل الـ principle
+
+49
+00:03:20,480 --> 00:03:24,260
+بيشتغل على مبدأ ان هذا الـ reagent يحتوي على كبر
+
+50
+00:03:24,260 --> 00:03:29,200
+صلفات على كبر صلفات الكبر هذا الموجود في الكبر
+
+51
+00:03:29,200 --> 00:03:34,920
+صلفات يعمل ك oxidizing agent بيروح بي acid السكر
+
+52
+00:03:35,220 --> 00:03:42,620
+والسكر بيشتغل ك reducing agent بيعمل اختزال للسكر
+
+53
+00:03:42,620 --> 00:03:47,340
+فالـ principle كيف هتكون على شكل المعادلات زي ما
+
+54
+00:03:47,340 --> 00:03:50,920
+أخدنا في المعمل الأول لو بدنا نعملها أنه عندي سكر
+
+55
+00:03:50,920 --> 00:03:54,120
+على فرض أنه aldehyde هو إما هيكون aldehyde أو
+
+56
+00:03:54,120 --> 00:03:57,720
+كيتون نعمل معادلة للـ aldehyde لحال ومعادلة
+
+57
+00:03:57,720 --> 00:04:03,440
+للكيتون لحال هو aldehydeزائد الكبركايون اللى موجود
+
+58
+00:04:03,440 --> 00:04:08,420
+فى الكبرسالفات فى وجود alkaline media alkaline pH
+
+59
+00:04:08,420 --> 00:04:12,740
+بيعطينا النتج الالتهلالدهايت هيصير له oxidation
+
+60
+00:04:12,740 --> 00:04:17,720
+تحول إلى كربوكسيلات طب والكبركايون هيصير له
+
+61
+00:04:17,720 --> 00:04:22,640
+reduction إلى كبرس اوكسايد بترسب على شكل كبرس
+
+62
+00:04:22,640 --> 00:04:28,680
+اوكسايدلونه brick red أحمر طوم فإذا الناتج أعطاني
+
+63
+00:04:28,680 --> 00:04:32,380
+لون اتحول اللون إلى brick red إذا هذا reducing
+
+64
+00:04:32,380 --> 00:04:37,480
+sugar إذا ماعطانيش إذا non-reducing sugar لو بدك
+
+65
+00:04:37,480 --> 00:04:42,410
+تبم عادلة تانية للكيتون، الكيتون ماءفي وزاقد
+
+66
+00:04:42,410 --> 00:04:46,810
+كبرسالفات اللي هو الـ Capric Ion في وجود alkaline
+
+67
+00:04:46,810 --> 00:04:50,510
+media الكيتون بدي يصير له oxidation بس بدي يتحول
+
+68
+00:04:50,510 --> 00:04:54,910
+إلى هيدروكس الكربوكسيلان طيب، والـ Capric Ion
+
+69
+00:04:54,910 --> 00:04:59,070
+بيترسب، بيصير له reduction، بترسب على شكل Caprous
+
+70
+00:04:59,070 --> 00:05:05,830
+Oxideلونه بريك ريب بريسبيتات بيحكيلي the color of
+
+71
+00:05:05,830 --> 00:05:10,130
+بريسبيتات بتعتمد شدة اللون بتعتمد على تركيز
+
+72
+00:05:10,130 --> 00:05:16,610
+reducing sugar كدهش في كمية من هذا السكةخطوات
+
+73
+00:05:16,610 --> 00:05:20,650
+إجراء التجربة هاي أني بحط واحد من من السكر اللي
+
+74
+00:05:20,650 --> 00:05:23,750
+موجود عندى مثلا عندي عدة سكريات سكر جلكوز،
+
+75
+00:05:23,750 --> 00:05:27,810
+فراكتوز، سكروز، لاكتوز، مالتوز، ودول بدكشف عنهم،
+
+76
+00:05:27,810 --> 00:05:31,310
+كل واحد بشتغلوا بتيوم بحط واحد من من هذا السكر
+
+77
+00:05:31,310 --> 00:05:34,930
+واضيف عليه واحد من من الـBindex Reagent من
+
+78
+00:05:34,930 --> 00:05:39,310
+الـReagent وبحطهم في الـwater bath لمدة خمس ديالي
+
+79
+00:05:39,880 --> 00:05:43,040
+بعدين بطلع إذا اتحول اللون إلى brick red إلى لون
+
+80
+00:05:43,040 --> 00:05:47,240
+أحمر إذا هذا reducing sugar إذا ما اتغيرش اللون
+
+81
+00:05:47,240 --> 00:05:52,020
+إذا هو non-reducing sugar أو إنه طلع لون تاني غير
+
+82
+00:05:52,020 --> 00:05:57,840
+ال brick red هو non-reducing sugarالفحص التاني
+
+83
+00:05:57,840 --> 00:06:02,760
+اسمه Barfeuil Test Barfeuil Test هذا إيش ال
+
+84
+00:06:02,760 --> 00:06:06,080
+objective منه؟ to distinguish between mono and
+
+85
+00:06:06,080 --> 00:06:09,960
+disaccharide بيفرق من السكريات الأحدية و الثنائية
+
+86
+00:06:09,960 --> 00:06:12,800
+يعني لو عندي سكر مش عارف هل هو mono ولا
+
+87
+00:06:12,800 --> 00:06:16,380
+disaccharide بستخدم فحص ال Barfeuil Test عشان
+
+88
+00:06:16,380 --> 00:06:20,060
+أفرقه هل هو mono ولا disaccharideكيف الـ principle
+
+89
+00:06:20,060 --> 00:06:25,320
+طريقة شغله الـ principle إنه الـ Parfaits reagent
+
+90
+00:06:25,320 --> 00:06:30,750
+اللي أنا بستخدمه بيتكون main coverage أيهطبعا عند
+
+91
+00:06:30,750 --> 00:06:34,850
+الـ Reagent بيحتوي على Capric Ion فأنا بحط السكر،
+
+92
+00:06:34,850 --> 00:06:38,370
+السكر هذا النوعيا هيكون Aldehyde يا كيتو مثلا
+
+93
+00:06:38,370 --> 00:06:41,890
+Aldehyde، نيني نكتب معادلة Aldehyde الـ Aldehyde
+
+94
+00:06:41,890 --> 00:06:44,730
+زائد الـ Capric Ion اللي موجود في الـ Reagent
+
+95
+00:06:44,730 --> 00:06:48,470
+بيتحول، بيطلع عندي النتج الـ Aldehyde بتحول
+
+96
+00:06:48,470 --> 00:06:54,940
+لكربوكسيلات و Capric Oxideأما إذا كان كيتون كيتون
+
+97
+00:06:54,940 --> 00:06:57,400
+باضيف عليه الـ reagent اللي هو الـ Capric Ion
+
+98
+00:06:57,400 --> 00:07:02,040
+بتحول الكيتون إلى Hydroxyl Carboxylate والـ Capric
+
+99
+00:07:02,040 --> 00:07:06,900
+Ion بتحول إلى Caprous Oxideاللون في الأخر في
+
+100
+00:07:06,900 --> 00:07:11,280
+المعادلتين اللون بيكون break red إذا اتحول إلى لون
+
+101
+00:07:11,280 --> 00:07:16,440
+break red بيكون هذا monosaccharide بيحكي في الـ
+
+102
+00:07:16,440 --> 00:07:18,900
+Parvoid reagent react with monosaccharide to
+
+103
+00:07:18,900 --> 00:07:21,940
+produce caprous oxide faster rate than
+
+104
+00:07:21,940 --> 00:07:25,120
+disaccharide ال Parvoid reagent بيتفاعل مع
+
+105
+00:07:25,120 --> 00:07:27,160
+الإتنين، مع الـ monosaccharide و مع الـ
+
+106
+00:07:27,160 --> 00:07:30,830
+disaccharideبيعطيني لون ولكن تفاعله مع الـ
+
+107
+00:07:30,830 --> 00:07:34,150
+monosaccharide أسرع من تفاعله مع الـ disaccharide
+
+108
+00:07:34,150 --> 00:07:38,590
+فاحنا لما نشتغل نحطه وقت معين لواجهة هذا بيسمح
+
+109
+00:07:38,590 --> 00:07:42,650
+بتفاعل فقط الـ monosaccharide لو أتأخرنا عليه ممكن
+
+110
+00:07:42,650 --> 00:07:46,010
+الـ disaccharide يتفاعل كمان فاحنا بدنا نحطه بس
+
+111
+00:07:46,010 --> 00:07:50,270
+لواجهة تفاعل الـ monosaccharide و بعد هيك بطلا إذا
+
+112
+00:07:50,270 --> 00:07:55,360
+تغيري اللون يعني هذا monosaccharideالـ principle
+
+113
+00:07:55,360 --> 00:08:00,580
+للبرفويدز زي الـ بين dicts ولكن بدون وجود وسط
+
+114
+00:08:00,580 --> 00:08:07,300
+قاعد، لا تحتاج إلى وجود وسط قاعدكيف طريقة العمل؟
+
+115
+00:08:07,300 --> 00:08:10,880
+احط واحد مل من السكر اللى موجود عندى على واحد مل
+
+116
+00:08:10,880 --> 00:08:15,040
+من الاجان ثم بحطه في ال water bath لمدة عشر دقائق
+
+117
+00:08:15,040 --> 00:08:18,060
+بطلع من ال water bath اللى اتكون عندى red
+
+118
+00:08:18,060 --> 00:08:21,540
+precipitate اللون الأحمر، الراسب اللون الأحمر، إذا
+
+119
+00:08:21,540 --> 00:08:25,600
+ده mono-saccharide إذا ما اتكونش، إذا هو dye
+
+120
+00:08:25,600 --> 00:08:31,990
+-saccharide أو سكر آخرفحص التالت هو فحص ال bios أو
+
+121
+00:08:31,990 --> 00:08:37,030
+اسمه تاني orsenol test bios or orsenol هذا خاص
+
+122
+00:08:37,030 --> 00:08:40,550
+للكشف عن ال pentose objective for the detection of
+
+123
+00:08:40,550 --> 00:08:43,710
+pentose it distinguishes between pentose and
+
+124
+00:08:43,710 --> 00:08:48,750
+hexose بيفرق بين الخماسي والسداسي هو للكشف عن ال
+
+125
+00:08:48,750 --> 00:08:53,570
+pentose عن الخماسي فبيفرق بين الخماسي والسداسي كيف
+
+126
+00:08:53,570 --> 00:08:58,010
+ال principle أنا بستخدم bios reagent bios reagent
+
+127
+00:08:58,340 --> 00:09:01,380
+اللي هو شو مكوناته هذا الـ Biles Region واحد من
+
+128
+00:09:01,380 --> 00:09:06,000
+عشرة في المية أورسينول Concentrated HCl واحد من
+
+129
+00:09:06,000 --> 00:09:11,930
+عشرة في المية فرق الكلورايسيت H2Oإن هاد الـ
+
+130
+00:09:11,930 --> 00:09:16,150
+Concentrated HCl بيعمل الـ dehydration للسُكّار
+
+131
+00:09:16,150 --> 00:09:20,230
+قلت الـ Biles بيفرق بين الـ Pentose و الـ Hexose
+
+132
+00:09:20,230 --> 00:09:24,010
+كيف بدي اتفعل الـ Principle بحط بيلز بحط السُكّار
+
+133
+00:09:24,010 --> 00:09:28,310
+أنا مثلا Pentose و بحط عليه الـ Biles reagent
+
+134
+00:09:28,310 --> 00:09:33,130
+المكونات التلاتة طبعا كلهم مخلطين بيتحول السُكّار
+
+135
+00:09:33,130 --> 00:09:37,990
+إلى حاجة اسمها Fair For Allفيرفورال و اورسينول
+
+136
+00:09:37,990 --> 00:09:42,590
+اللي ضال عندنا بتحول السكر إلى فيرفورال فيرفورال
+
+137
+00:09:42,590 --> 00:09:47,650
+ماء اورسينول في وجود فرك ايون من الفرك كلورايت جاي
+
+138
+00:09:47,650 --> 00:09:55,030
+بتكون عندى لون blue to green لون أخضر مزرقأو
+
+139
+00:09:55,030 --> 00:09:58,910
+precipitate على شكل هذا اللون اللي هو أخضر مزرق
+
+140
+00:09:58,910 --> 00:10:02,610
+بيطلع عندي إذا طلع هذا اللون إذا الموجود بنتاز
+
+141
+00:10:02,610 --> 00:10:06,490
+بيحكي لي إذا طولت عليه شهو يسار اللون براون بتكون
+
+142
+00:10:06,490 --> 00:10:10,370
+لون براون precipitate لون راسف لونه بني بيكون
+
+143
+00:10:10,370 --> 00:10:16,240
+الموجود hexoseفلو بتدخل دلخص معادلة الـ Biles على
+
+144
+00:10:16,240 --> 00:10:19,720
+شكل معادلة، دلخص الـ principle على شكل معادلة، انه
+
+145
+00:10:19,720 --> 00:10:21,740
+الـ Pentose، هذا الفحص كان للـ Pentose، الـ
+
+146
+00:10:21,740 --> 00:10:26,240
+Pentose في وجود HCl، أول حاجة بتشتغل من الريجط
+
+147
+00:10:26,240 --> 00:10:30,200
+اللي هو الـ HCl، بيعمل dehydration للسجر، فبينتج
+
+148
+00:10:30,200 --> 00:10:35,620
+عنه، بيتحول إلى Ferfolan زاد Orsinol زاد Ferric
+
+149
+00:10:35,620 --> 00:10:40,740
+Chlorideأذا أنا عند الـ Pentose في وجود HCl
+
+150
+00:10:40,740 --> 00:10:46,620
+بيعطيني Fervorol زي اورسينول زي تفرج الجلورال هدول
+
+151
+00:10:46,620 --> 00:10:50,100
+التلاتة مع بعض هاليات بيتفعلوا بيعطوني Complex
+
+152
+00:10:50,100 --> 00:10:57,060
+لونه blue to green Complex لونه blue to greenكيف
+
+153
+00:10:57,060 --> 00:11:02,320
+خطوات العمل بحط واحد مل من السكر على واحد و نص مل
+
+154
+00:11:02,320 --> 00:11:07,320
+من ال bios reagent وبعملهم خلط mix واحد مل من
+
+155
+00:11:07,320 --> 00:11:11,020
+السكر على واحد و نص مل من ال reagent بعد هيك
+
+156
+00:11:11,020 --> 00:11:14,180
+بسخنها على اللهب مش في ال water bath هي على اللهب
+
+157
+00:11:14,180 --> 00:11:18,420
+بسخنها لحد ما تغلي بعد ما تغلي بحطها بالراكد تبرد
+
+158
+00:11:18,420 --> 00:11:21,960
+إذا اتحول اللون إلى blue to green is positive إذا
+
+159
+00:11:21,960 --> 00:11:25,540
+السكر اللي موجود عندى بنتوتز إذا ماتحولش إذا
+
+160
+00:11:25,540 --> 00:11:31,050
+negativeالفحص اللي بعد هي اسمه Silvanov او RIS
+
+161
+00:11:31,050 --> 00:11:35,790
+Arsenal Test Silvanov او RIS Arsenal Test ايش
+
+162
+00:11:35,790 --> 00:11:39,110
+الهدف من هذا الفحص for detection of ketosis يكشف
+
+163
+00:11:39,110 --> 00:11:42,930
+عن الـ ketosis السكر اللي يحتوي على كيتون اجرم
+
+164
+00:11:44,450 --> 00:11:48,850
+البرنسيبل سيلوانوف تيست two distinguish between
+
+165
+00:11:48,850 --> 00:11:52,290
+hexose with a ketone group and hexose with
+
+166
+00:11:52,290 --> 00:11:55,610
+aldehyde group هو الكشف بيفرق بين اللي عنده
+
+167
+00:11:55,610 --> 00:11:59,810
+aldehyde عن اللي عنده كيتون ويكونه hexose يكون
+
+168
+00:11:59,810 --> 00:12:06,070
+سداثيهذا الفحص لو اشتغلتي مع كيتوز، مع كيتوز اللي
+
+169
+00:12:06,070 --> 00:12:10,070
+عنده كيتون جروب، بيعطيكي لون Red، هيعطيكي لون أحمر
+
+170
+00:12:10,070 --> 00:12:13,390
+وبيتكون بسرعة، it's formed rapidly، إذا اتكون لون
+
+171
+00:12:13,390 --> 00:12:17,590
+أحمر بسرعة، إذا اللي موجود كيتون، طيب الالدوز
+
+172
+00:12:17,590 --> 00:12:21,410
+بتعطيني light pink color و بتاخد واجهة أسرع، واجهة
+
+173
+00:12:21,410 --> 00:12:26,510
+أطول، longer timeإذا اتكون عندي اللون البنك وبواجه
+
+174
+00:12:26,510 --> 00:12:29,790
+ا طويل لما اتكون إذا اللي موجود عندي الـdose
+
+175
+00:12:29,790 --> 00:12:36,170
+الـketohexose يعني هو سداسي وعنده ketone group زي
+
+176
+00:12:36,170 --> 00:12:42,370
+الـfructose وعنا فيه حاجة di-saccharide وأحد
+
+177
+00:12:42,370 --> 00:12:45,870
+مكوناته يكون الـketohexose فجيت الـdi-saccharide
+
+178
+00:12:45,870 --> 00:12:49,410
+زي السكروز هو عبارة عن انتحار جليكوز مع
+
+179
+00:12:49,410 --> 00:12:53,860
+الـfructoseالـ fractose يعتبر ketohexose فأحد
+
+180
+00:12:53,860 --> 00:12:59,840
+مكونات السكاروز هو fractose وهو ketohexose يعني
+
+181
+00:12:59,840 --> 00:13:04,160
+هيعطيني positive مع هذا الفحص ولكن بدرجة أخف لللون
+
+182
+00:13:04,160 --> 00:13:09,660
+هيعطيني لون cherry red أحمر كرزي أما السكريات
+
+183
+00:13:09,660 --> 00:13:12,280
+الأخرى اللي هي الـdose اللي عندهاش ketone group
+
+184
+00:13:12,280 --> 00:13:16,500
+عندها aldehyde group تعطيني لون من yellow to pink
+
+185
+00:13:16,500 --> 00:13:22,920
+from yellow to pinkالـ principle مبتدأ العمل أنه
+
+186
+00:13:22,920 --> 00:13:26,080
+في عندي reaction تسمى الـ cellulose reaction اللي
+
+187
+00:13:26,080 --> 00:13:29,620
+هو عبارة عن خمسة من عشرة في المية risk of arsenol
+
+188
+00:13:29,620 --> 00:13:34,140
+في تلاتة normal HCl مجرد ما أضيف هذا ال reaction
+
+189
+00:13:34,140 --> 00:13:39,540
+بتيجي الـ concentrated HCl أولا شىء هذا الفحص للـ
+
+190
+00:13:39,540 --> 00:13:43,520
+ketose فعندي ketohexose الـ ketohexose في وجود الـ
+
+191
+00:13:43,520 --> 00:13:47,280
+HCl اللي موجودة في ال reaction بتعمل dehydration
+
+192
+00:13:47,280 --> 00:13:51,890
+للسكربتحولوا إلى مشتقات الفيرفورال اللي هي
+
+193
+00:13:51,890 --> 00:13:56,570
+Hydroxymethylferforate Hydroxy بتحول الـ Ketose
+
+194
+00:13:56,570 --> 00:14:01,950
+إلى Hydroxymethylferforate في وجود الـ HCl فالجاتب
+
+195
+00:14:01,950 --> 00:14:05,110
+بيجي الـ Hydroxymethylferforate بيتفاعل مع الـ
+
+196
+00:14:05,110 --> 00:14:09,250
+Resorcinol اللي في الرياجان بيعطيني Complex لونه
+
+197
+00:14:09,250 --> 00:14:15,950
+Cherry Red لون أحمر كرزي أو لون Redوهذا التفاعل
+
+198
+00:14:15,950 --> 00:14:19,190
+بيشتغل بسرعة خلال two minutes within two minutes
+
+199
+00:14:19,190 --> 00:14:23,710
+أما الالدوهيكسوز بتتفاعل بشكل بطيء مع هذا وبتكون
+
+200
+00:14:23,710 --> 00:14:27,470
+نفس الناتج ولكن بيكون أبطأ إذا ال principle لو
+
+201
+00:14:27,470 --> 00:14:32,610
+بتعملها على شكل معادلة بحكي كيتوهيكسوز في وجود HCl
+
+202
+00:14:32,610 --> 00:14:37,170
+بيعطيني Hydroxyl Methyl Ferforyl بيتفاعل مع
+
+203
+00:14:37,170 --> 00:14:43,450
+الرسورسينول بيعطيني Complex Lonoredكيف خطوات إجراء
+
+204
+00:14:43,450 --> 00:14:47,970
+التجربة؟ بحط تلاتة مل من الاجن على drop واحدة من
+
+205
+00:14:47,970 --> 00:14:52,010
+السكة بعدين بحطه في ال water bath لمدة تلاتة مل
+
+206
+00:14:52,010 --> 00:14:55,670
+بعدين بقلق إذا اتكون عندى اللون ال red إذا هذا
+
+207
+00:14:55,670 --> 00:15:00,470
+positive وهذا عبارة عن كيتوز إذا ما اتكونش إذا
+
+208
+00:15:00,470 --> 00:15:06,030
+negativeآخر فحص هو الـ Bicric Acid Test فحص الـ
+
+209
+00:15:06,030 --> 00:15:09,990
+Bicric Acid الـ objective منه يستخدم للكشف عن الـ
+
+210
+00:15:09,990 --> 00:15:13,810
+reducing sugar يعني زيه زي أول فحص اللي هو الـ
+
+211
+00:15:13,810 --> 00:15:18,390
+Vindict Test للكشف عن الـ reducing sugar زي أول
+
+212
+00:15:18,390 --> 00:15:22,670
+فحص اللي هو الـ Vindict Test كيف ال principle أنه
+
+213
+00:15:22,670 --> 00:15:26,470
+عندي Bicric Acid اللي هو اسمه تاني اتنين اربع ستة
+
+214
+00:15:26,470 --> 00:15:30,990
+Trinitrophenol Bicric Acid هو اتنين اربع ستة
+
+215
+00:15:30,990 --> 00:15:35,680
+Trinitrophenolأو الـ TMP بيجي هذا البكريك أسد
+
+216
+00:15:35,680 --> 00:15:40,260
+بيتفعل مع الـ Reducing Sugar وبيصير لو واحد فيهم
+
+217
+00:15:40,260 --> 00:15:44,780
+أكسدة وواحد فيهم اختزال وبيتكون عندي الـ Red Color
+
+218
+00:15:44,780 --> 00:15:50,760
+جاي من وين هذا الـ Red Color من عملية اختزال
+
+219
+00:15:50,760 --> 00:15:57,500
+البكريك أسد إلى Sodium Bicaramideالـ Red color زي
+
+220
+00:15:57,500 --> 00:16:00,960
+من حدوث reduction للـ Bacric Acid لأنه بيصيبله
+
+221
+00:16:00,960 --> 00:16:04,980
+اختزان، بتحول لـ Sodium Bicaramide فلو بدك تكتب
+
+222
+00:16:04,980 --> 00:16:08,400
+معادلة للـ Principle للـ Bacric Acid أن عندي
+
+223
+00:16:08,400 --> 00:16:13,660
+Aldehyde زائد Bacric Acid في وجود أنيوات، في وجود
+
+224
+00:16:13,660 --> 00:16:19,270
+قاعدةالـ aldehyde بيصير له oxidation بيتأكسد إلى
+
+225
+00:16:19,270 --> 00:16:22,790
+حاجة اسمها الـ carboxylate حكينا الـ bicaric acid
+
+226
+00:16:22,790 --> 00:16:26,390
+بيصير له reduction إلى أيش؟ إلى Sodium Bicaramate
+
+227
+00:16:26,390 --> 00:16:30,010
+Sodium Bicaramate هذا له نورين، طب إذا كيتون مش
+
+228
+00:16:30,010 --> 00:16:34,380
+aldehyde؟الكيتون زاد الـbicaric acid في وجود
+
+229
+00:16:34,380 --> 00:16:38,280
+انيواتش الكيتون إيش هيصير له oxidation إلى
+
+230
+00:16:38,280 --> 00:16:43,020
+هيدروكسلكربوكسيلات والبيكريك أسن بيصير له
+
+231
+00:16:43,020 --> 00:16:47,800
+reduction إلى صوديوم بكرامات لونه red إذا اتكون
+
+232
+00:16:47,800 --> 00:16:51,820
+اللون ال red إذا هو reducing sugar إذا ما اتكونش
+
+233
+00:16:51,820 --> 00:16:58,080
+إذا هو non-reducing sugar كيف خطوات العمل بحط واحد
+
+234
+00:16:58,080 --> 00:17:03,750
+مل من السكرعلى 1 مل من الـ Bacric Acid على نص مل
+
+235
+00:17:03,750 --> 00:17:07,230
+من الـ Honey Watch من القاعدة و بعدين بحطه في الـ
+
+236
+00:17:07,230 --> 00:17:12,030
+Water Bath لمدة 5 دقائق، لمدة 5 دقائق إذا ظهر بعد
+
+237
+00:17:12,030 --> 00:17:15,470
+ما طلع من الـ Water Bath كان اللون Red إذا هو
+
+238
+00:17:15,470 --> 00:17:20,310
+عبارة عن Reducing Sugar إذا اللون ما اتكونش اللون
+
+239
+00:17:20,310 --> 00:17:25,050
+الـ Red إذا هو Non-Reducing Sugar بهيك بنكون خلصنا
+
+240
+00:17:25,050 --> 00:17:29,980
+تجارب الفحص و الكشف عن السكرياتمطلوب منكم تكتبولي
+
+241
+00:17:29,980 --> 00:17:34,960
+ال principle لكل فحص على شكل معادلة زي ما عملنا في
+
+242
+00:17:34,960 --> 00:17:38,420
+فحص qualitative test of amino acid
+
diff --git a/PL9fwy3NUQKwZ1Q7zOg1ObgufPMjRI3WY1/6BIvAPIvRuM_raw.json b/PL9fwy3NUQKwZ1Q7zOg1ObgufPMjRI3WY1/6BIvAPIvRuM_raw.json
new file mode 100644
index 0000000000000000000000000000000000000000..b022aae0932356de320ec7484599cda7a1e47106
--- /dev/null
+++ b/PL9fwy3NUQKwZ1Q7zOg1ObgufPMjRI3WY1/6BIvAPIvRuM_raw.json
@@ -0,0 +1 @@
+{"segments": [{"id": 1, "seek": 2892, "start": 0.0, "end": 28.92, "text": "هنا عندنا جدول بيقارن بين أنواع الـOxidizing agent المستخدم نقارن بين الـBendix solution و الـFilling solution و الـTolens reagent أولا يجي من ناحية المكونات الـBendix بيتكون من كبر صلفات في ألكالاين زيتريا دوّب في ألكالاين زيترا الفilling بيتكون من كبر صلفات في ألكالاين ترتريا الـTolens بيتكون من silver nitrate إن أكي و أسامانة", "tokens": [3224, 8315, 43242, 8315, 10874, 3215, 12610, 4724, 1829, 4587, 9640, 1863, 49374, 14739, 14407, 3615, 2423, 39184, 46, 87, 327, 3319, 9461, 9673, 14851, 9778, 40448, 8717, 4587, 9640, 1863, 49374, 2423, 39184, 33, 521, 970, 3827, 4032, 2423, 39184, 37, 7345, 3827, 4032, 2423, 39184, 51, 401, 694, 26949, 317, 5551, 12610, 995, 7251, 7435, 1829, 9154, 8717, 39319, 10632, 9673, 4117, 2407, 8315, 2655, 2423, 39184, 33, 521, 970, 4724, 36081, 30544, 9154, 9122, 26890, 20328, 46538, 9307, 8978, 5551, 1211, 4117, 6027, 995, 9957, 30767, 36081, 16572, 995, 11778, 2407, 11703, 3555, 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"probability": 0.87548828125}, {"start": 312.49, "end": 313.13, "word": " من", "probability": 0.982421875}, {"start": 313.13, "end": 313.75, "word": " هذا", "probability": 0.927734375}, {"start": 313.75, "end": 314.57, "word": " السكة", "probability": 0.7281087239583334}], "temperature": 1.0}, {"id": 15, "seek": 33931, "start": 316.03, "end": 339.31, "text": "خطوات إجراء التجربة هاي أني بحط واحد من من السكر اللي موجود عندى مثلا عندي عدة سكريات سكر جلكوز، فراكتوز، سكروز، لاكتوز، مالتوز، ودول بدكشف عنهم، كل واحد بشتغلوا بتيوم بحط واحد من من هذا السكر واضيف عليه واحد من من الـBindex Reagent من الـReagent وبحطهم في الـwater bath لمدة خمس ديالي", "tokens": [9778, 9566, 2407, 9307, 11933, 7435, 2288, 16606, 16712, 7435, 25513, 3660, 8032, 47302, 14739, 1829, 4724, 5016, 9566, 36764, 24401, 9154, 9154, 21136, 37983, 13672, 1829, 3714, 29245, 23328, 43242, 7578, 50113, 15040, 18871, 16254, 6225, 41891, 8608, 4117, 16572, 9307, 8608, 37983, 10874, 23275, 2407, 11622, 12399, 6156, 23557, 4117, 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هذا إيش ال objective منه؟ to distinguish between mono and disaccharide بيفرق من السكريات الأحدية و الثنائية يعني لو عندي سكر مش عارف هل هو mono ولا disaccharide بستخدم فحص ال Barfeuil Test عشان أفرقه هل هو mono ولا disaccharide", "tokens": [6027, 5172, 5016, 9381, 16712, 7649, 1829, 24525, 2304, 3224, 4156, 2106, 84, 388, 9279, 4156, 2106, 84, 388, 9279, 23758, 11933, 1829, 8592, 2423, 10024, 9154, 3224, 22807, 281, 20206, 1296, 35624, 293, 717, 326, 7374, 482, 4724, 33911, 2288, 4587, 9154, 21136, 4117, 16572, 9307, 16247, 24401, 10632, 4032, 6024, 104, 1863, 16373, 10632, 37495, 22653, 45164, 18871, 16254, 8608, 37983, 37893, 6225, 9640, 5172, 8032, 1211, 31439, 35624, 49429, 717, 326, 7374, 482, 4724, 14851, 9778, 40448, 6156, 5016, 9381, 2423, 4156, 2106, 84, 388, 9279, 6225, 8592, 7649, 5551, 5172, 2288, 4587, 3224, 8032, 1211, 31439, 35624, 49429, 717, 326, 7374, 482], "avg_logprob": -0.25000000557052754, "compression_ratio": 1.7219730941704037, "no_speech_prob": 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principle طريقة شغله الـ principle إنه الـ Parfaits reagent اللي أنا بستخدمه بيتكون main coverage أيه", "tokens": [4117, 33911, 2423, 39184, 8665, 23032, 16572, 28671, 13412, 17082, 43761, 2423, 39184, 8665, 36145, 3224, 2423, 39184, 3457, 69, 1001, 82, 26949, 317, 13672, 1829, 41850, 4724, 14851, 9778, 40448, 3224, 4724, 36081, 30544, 2135, 2060, 559, 68, 36632, 3224], "avg_logprob": -0.40104166240919203, "compression_ratio": 1.2580645161290323, "no_speech_prob": 0.0, "words": [{"start": 378.86, "end": 379.28, "word": "كيف", "probability": 0.64697265625}, {"start": 379.28, "end": 379.54, "word": " الـ", "probability": 0.548828125}, {"start": 379.54, "end": 380.06, "word": " principle", "probability": 0.453125}, {"start": 380.06, "end": 380.6, "word": " طريقة", "probability": 0.7657877604166666}, {"start": 380.6, "end": 381.1, "word": " شغله", "probability": 0.9593098958333334}, {"start": 381.1, "end": 381.94, "word": " الـ", "probability": 0.56591796875}, {"start": 381.94, "end": 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{"start": 677.48, "end": 677.98, "word": " بالراكد", "probability": 0.613800048828125}, {"start": 677.98, "end": 678.42, "word": " تبرد", "probability": 0.8961588541666666}, {"start": 678.42, "end": 678.9, "word": " إذا", "probability": 0.8291015625}, {"start": 678.9, "end": 679.36, "word": " اتحول", "probability": 0.86767578125}, {"start": 679.36, "end": 679.72, "word": " اللون", "probability": 0.9677734375}, {"start": 679.72, "end": 679.92, "word": " إلى", "probability": 0.603515625}, {"start": 679.92, "end": 680.16, "word": " blue", "probability": 0.97802734375}, {"start": 680.16, "end": 680.38, "word": " to", "probability": 0.95751953125}, {"start": 680.38, "end": 680.74, "word": " green", "probability": 0.94140625}, {"start": 680.74, "end": 680.98, "word": " is", "probability": 0.73095703125}, {"start": 680.98, "end": 681.5, "word": " positive", "probability": 0.207763671875}, {"start": 681.5, "end": 681.96, "word": " إذا", "probability": 0.7244873046875}, {"start": 681.96, "end": 682.38, "word": " السكر", "probability": 0.976806640625}, {"start": 682.38, "end": 682.5, "word": " اللي", "probability": 0.767333984375}, {"start": 682.5, "end": 682.86, "word": " موجود", "probability": 0.9733072916666666}, {"start": 682.86, "end": 683.18, "word": " عندى", "probability": 0.631591796875}, {"start": 683.18, "end": 684.26, "word": " بنتوتز", "probability": 0.507049560546875}, {"start": 684.26, "end": 684.5, "word": " إذا", "probability": 0.951904296875}, {"start": 684.5, "end": 685.24, "word": " ماتحولش", "probability": 0.800341796875}, {"start": 685.24, "end": 685.54, "word": " إذا", "probability": 0.855712890625}, {"start": 685.54, "end": 686.14, "word": " negative", "probability": 0.78271484375}], "temperature": 1.0}, {"id": 33, "seek": 70293, "start": 688.09, "end": 702.93, "text": "الفحص اللي بعد هي اسمه Silvanov او RIS Arsenal Test Silvanov او RIS Arsenal Test ايش الهدف من هذا الفحص for detection of ketosis يكشف عن الـ ketosis السكر اللي يحتوي على كيتون اجرم", "tokens": [6027, 5172, 5016, 9381, 13672, 1829, 39182, 39896, 24525, 2304, 3224, 6943, 85, 38990, 1975, 2407, 497, 2343, 49156, 9279, 6943, 85, 38990, 1975, 2407, 497, 2343, 49156, 9279, 1975, 1829, 8592, 2423, 3224, 3215, 5172, 9154, 23758, 27188, 5016, 9381, 337, 17784, 295, 14979, 8211, 7251, 4117, 8592, 5172, 18871, 2423, 39184, 14979, 8211, 21136, 37983, 13672, 1829, 7251, 33753, 45865, 15844, 9122, 36081, 11536, 1975, 7435, 2288, 2304], "avg_logprob": -0.275528158100558, "compression_ratio": 1.5, "no_speech_prob": 8.940696716308594e-07, "words": [{"start": 688.09, "end": 688.51, "word": "الفحص", "probability": 0.8070068359375}, {"start": 688.51, "end": 688.71, "word": " اللي", "probability": 0.7490234375}, {"start": 688.71, "end": 688.93, "word": " بعد", "probability": 0.84423828125}, {"start": 688.93, "end": 689.15, "word": " هي", "probability": 0.96337890625}, {"start": 689.15, "end": 689.51, "word": " اسمه", "probability": 0.9685872395833334}, {"start": 689.51, "end": 690.31, 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"probability": 0.95263671875}, {"start": 696.27, "end": 696.43, "word": " هذا", "probability": 0.89599609375}, {"start": 696.43, "end": 696.87, "word": " الفحص", "probability": 0.97119140625}, {"start": 696.87, "end": 697.07, "word": " for", "probability": 0.446044921875}, {"start": 697.07, "end": 697.59, "word": " detection", "probability": 0.93408203125}, {"start": 697.59, "end": 697.91, "word": " of", "probability": 0.98193359375}, {"start": 697.91, "end": 698.49, "word": " ketosis", "probability": 0.68310546875}, {"start": 698.49, "end": 699.11, "word": " يكشف", "probability": 0.8572998046875}, {"start": 699.11, "end": 699.37, "word": " عن", "probability": 0.99560546875}, {"start": 699.37, "end": 699.65, "word": " الـ", "probability": 0.6048583984375}, {"start": 699.65, "end": 700.21, "word": " ketosis", "probability": 0.5863037109375}, {"start": 700.21, "end": 701.07, "word": " السكر", "probability": 0.7919921875}, {"start": 701.07, "end": 701.23, "word": " اللي", "probability": 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إذا اتكون لون أحمر بسرعة، إذا اللي موجود كيتون، طيب الالدوز بتعطيني light pink color و بتاخد واجهة أسرع، واجهة أطول، longer time", "tokens": [3224, 15730, 27188, 5016, 9381, 45164, 1975, 8592, 2655, 17082, 1211, 31371, 20449, 9122, 36081, 2407, 11622, 12399, 20449, 9122, 36081, 2407, 11622, 13672, 1829, 43242, 3224, 9122, 36081, 11536, 10874, 32887, 3555, 12399, 4724, 40228, 9566, 1829, 4117, 1829, 5296, 11536, 4477, 12399, 39896, 3615, 9566, 1829, 4117, 1829, 5296, 11536, 5551, 5016, 29973, 4032, 21292, 2655, 4117, 2407, 1863, 4724, 3794, 2288, 27884, 12399, 309, 311, 8693, 12910, 12399, 11933, 15730, 1975, 2655, 30544, 5296, 11536, 5551, 5016, 29973, 4724, 3794, 2288, 27884, 12399, 11933, 15730, 13672, 1829, 3714, 29245, 23328, 9122, 36081, 11536, 12399, 23032, 1829, 3555, 2423, 6027, 3215, 2407, 11622, 39894, 3615, 9566, 9957, 1829, 1442, 7022, 2017, 4032, 39894, 47283, 3215, 4032, 26108, 3224, 3660, 5551, 3794, 2288, 3615, 12399, 4032, 26108, 3224, 3660, 5551, 9566, 12610, 12399, 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" كيتون", "probability": 0.77392578125}, {"start": 726.65, "end": 727.03, "word": " جروب،", "probability": 0.6968994140625}, {"start": 727.03, "end": 727.63, "word": " بيعطيكي", "probability": 0.8924967447916666}, {"start": 727.63, "end": 727.93, "word": " لون", "probability": 0.972412109375}, {"start": 727.93, "end": 729.01, "word": " Red،", "probability": 0.5732421875}, {"start": 729.01, "end": 729.45, "word": " هيعطيكي", "probability": 0.9058837890625}, {"start": 729.45, "end": 729.61, "word": " لون", "probability": 0.98291015625}, {"start": 729.61, "end": 730.07, "word": " أحمر", "probability": 0.9451497395833334}, {"start": 730.07, "end": 730.71, "word": " وبيتكون", "probability": 0.7710774739583334}, {"start": 730.71, "end": 731.17, "word": " بسرعة،", "probability": 0.91611328125}, {"start": 731.17, "end": 731.35, "word": " it's", "probability": 0.6826171875}, {"start": 731.35, "end": 731.73, "word": " formed", "probability": 0.8662109375}, {"start": 731.73, "end": 732.79, "word": " rapidly،", "probability": 0.820068359375}, {"start": 732.79, "end": 732.93, "word": " إذا", "probability": 0.6282958984375}, {"start": 732.93, "end": 733.23, "word": " اتكون", "probability": 0.7698567708333334}, {"start": 733.23, "end": 733.39, "word": " لون", "probability": 0.981201171875}, {"start": 733.39, "end": 733.77, "word": " أحمر", "probability": 0.9851888020833334}, {"start": 733.77, "end": 734.21, "word": " بسرعة،", "probability": 0.96826171875}, {"start": 734.21, "end": 734.35, "word": " إذا", "probability": 0.706787109375}, {"start": 734.35, "end": 734.53, "word": " اللي", "probability": 0.58734130859375}, {"start": 734.53, "end": 735.01, "word": " موجود", "probability": 0.9847005208333334}, {"start": 735.01, "end": 736.11, "word": " كيتون،", "probability": 0.844482421875}, {"start": 736.11, "end": 736.55, "word": " طيب", "probability": 0.9025065104166666}, {"start": 736.55, "end": 737.59, "word": " الالدوز", "probability": 0.74716796875}, {"start": 737.59, "end": 738.21, "word": " بتعطيني", "probability": 0.9423828125}, {"start": 738.21, "end": 738.57, "word": " light", "probability": 0.72802734375}, {"start": 738.57, "end": 738.95, "word": " pink", "probability": 0.951171875}, {"start": 738.95, "end": 739.35, "word": " color", "probability": 0.92626953125}, {"start": 739.35, "end": 739.51, "word": " و", "probability": 0.52001953125}, {"start": 739.51, "end": 739.93, "word": " بتاخد", "probability": 0.9358723958333334}, {"start": 739.93, "end": 740.33, "word": " واجهة", "probability": 0.803955078125}, {"start": 740.33, "end": 740.93, "word": " أسرع،", "probability": 0.93564453125}, {"start": 740.93, "end": 741.41, "word": " واجهة", "probability": 0.80987548828125}, {"start": 741.41, "end": 741.89, "word": " أطول،", "probability": 0.9658203125}, {"start": 741.89, "end": 742.21, "word": " longer", "probability": 0.93408203125}, {"start": 742.21, "end": 742.63, "word": " time", "probability": 0.79443359375}], "temperature": 1.0}, {"id": 36, 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0.82392578125}], "temperature": 1.0}, {"id": 46, "seek": 101912, "start": 991.0, "end": 1019.12, "text": "الكيتون زاد الـbicaric acid في وجود انيواتش الكيتون إيش هيصير له oxidation إلى هيدروكسلكربوكسيلات والبيكريك أسن بيصير له reduction إلى صوديوم بكرامات لونه red إذا اتكون اللون ال red إذا هو reducing sugar إذا ما اتكونش إذا هو non-reducing sugar كيف خطوات العمل بحط واحد مل من السكر", "tokens": [6027, 4117, 36081, 11536, 30767, 18513, 2423, 39184, 65, 7953, 299, 8258, 8978, 49610, 23328, 16472, 1829, 2407, 9307, 8592, 33251, 36081, 11536, 11933, 1829, 8592, 39896, 9381, 13546, 46740, 36767, 30731, 8032, 25708, 2288, 2407, 4117, 3794, 23275, 25513, 2407, 4117, 3794, 26895, 9307, 16070, 3555, 1829, 4117, 16572, 4117, 5551, 3794, 1863, 4724, 1829, 9381, 13546, 46740, 11004, 30731, 20328, 2407, 16254, 20498, 4724, 4117, 2288, 10943, 9307, 5296, 11536, 3224, 2182, 11933, 15730, 1975, 2655, 30544, 13672, 11536, 2423, 2182, 11933, 15730, 31439, 12245, 5076, 11933, 15730, 19446, 1975, 2655, 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"word": " إيش", "probability": 0.6970621744791666}, {"start": 996.38, "end": 996.82, "word": " هيصير", "probability": 0.8746744791666666}, {"start": 996.82, "end": 996.96, "word": " له", "probability": 0.495849609375}, {"start": 996.96, "end": 997.7, "word": " oxidation", "probability": 0.88916015625}, {"start": 997.7, "end": 998.28, "word": " إلى", "probability": 0.921875}, {"start": 998.28, "end": 1000.18, "word": " هيدروكسلكربوكسيلات", "probability": 0.7044959435096154}, {"start": 1000.18, "end": 1001.5, "word": " والبيكريك", "probability": 0.5887451171875}, {"start": 1001.5, "end": 1002.0, "word": " أسن", "probability": 0.8009440104166666}, {"start": 1002.0, "end": 1002.86, "word": " بيصير", "probability": 0.98046875}, {"start": 1002.86, "end": 1003.02, "word": " له", "probability": 0.85400390625}, {"start": 1003.02, "end": 1003.54, "word": " reduction", "probability": 0.97705078125}, {"start": 1003.54, "end": 1004.2, "word": " إلى", "probability": 0.982421875}, {"start": 1004.2, "end": 1005.12, "word": " صوديوم", "probability": 0.7032470703125}, {"start": 1005.12, "end": 1005.94, "word": " بكرامات", "probability": 0.78115234375}, {"start": 1005.94, "end": 1006.78, "word": " لونه", "probability": 0.9586588541666666}, {"start": 1006.78, "end": 1007.02, "word": " red", "probability": 0.564453125}, {"start": 1007.02, "end": 1007.52, "word": " إذا", "probability": 0.6123046875}, {"start": 1007.52, "end": 1007.8, "word": " اتكون", "probability": 0.6553548177083334}, {"start": 1007.8, "end": 1008.24, "word": " اللون", "probability": 0.9736328125}, {"start": 1008.24, "end": 1008.4, "word": " ال", "probability": 0.94775390625}, {"start": 1008.4, "end": 1008.64, "word": " red", "probability": 0.45849609375}, {"start": 1008.64, "end": 1008.94, "word": " إذا", "probability": 0.6826171875}, {"start": 1008.94, "end": 1009.2, "word": " هو", "probability": 0.8076171875}, {"start": 1009.2, "end": 1009.8, "word": " reducing", "probability": 0.8525390625}, {"start": 1009.8, "end": 1010.34, "word": " sugar", "probability": 0.77880859375}, {"start": 1010.34, "end": 1010.98, "word": " إذا", "probability": 0.979248046875}, {"start": 1010.98, "end": 1011.12, "word": " ما", "probability": 0.78857421875}, {"start": 1011.12, "end": 1011.82, "word": " اتكونش", "probability": 0.8436279296875}, {"start": 1011.82, "end": 1012.12, "word": " إذا", "probability": 0.926025390625}, {"start": 1012.12, "end": 1012.42, "word": " هو", "probability": 0.9560546875}, {"start": 1012.42, "end": 1012.76, "word": " non", "probability": 0.71630859375}, {"start": 1012.76, "end": 1013.8, "word": "-reducing", "probability": 0.8250732421875}, {"start": 1013.8, "end": 1014.64, "word": " sugar", "probability": 0.84130859375}, {"start": 1014.64, "end": 1015.86, "word": " كيف", "probability": 0.968994140625}, {"start": 1015.86, "end": 1016.38, "word": " خطوات", "probability": 0.9620361328125}, {"start": 1016.38, "end": 1016.86, "word": " العمل", "probability": 0.990234375}, {"start": 1016.86, "end": 1017.68, "word": " بحط", "probability": 0.781494140625}, {"start": 1017.68, "end": 1018.08, "word": " واحد", "probability": 0.911865234375}, {"start": 1018.08, "end": 1018.38, "word": " مل", "probability": 0.88671875}, {"start": 1018.38, "end": 1018.58, "word": " من", "probability": 0.984375}, {"start": 1018.58, "end": 1019.12, "word": " السكر", "probability": 0.818115234375}], "temperature": 1.0}, {"id": 47, "seek": 104791, "start": 1019.65, "end": 1047.91, "text": "على 1 مل من الـ Bacric Acid على نص مل من الـ Honey Watch من القاعدة و بعدين بحطه في الـ Water Bath لمدة 5 دقائق، لمدة 5 دقائق إذا ظهر بعد ما طلع من الـ Water Bath كان اللون Red إذا هو عبارة عن Reducing Sugar إذا اللون ما اتكونش اللون الـ Red إذا هو Non-Reducing Sugar بهيك بنكون خلصنا تجارب الفحص و الكشف عن السكريات", "tokens": [3615, 23942, 502, 3714, 1211, 9154, 2423, 39184, 363, 326, 1341, 5097, 327, 15844, 8717, 9381, 3714, 1211, 9154, 2423, 39184, 16187, 7277, 9154, 25062, 995, 22488, 3660, 4032, 39182, 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"word": " مل", "probability": 0.8251953125}, {"start": 1020.83, "end": 1021.05, "word": " من", "probability": 0.9560546875}, {"start": 1021.05, "end": 1021.21, "word": " الـ", "probability": 0.891357421875}, {"start": 1021.21, "end": 1021.57, "word": " Bacric", "probability": 0.40283203125}, {"start": 1021.57, "end": 1022.15, "word": " Acid", "probability": 0.875244140625}, {"start": 1022.15, "end": 1022.87, "word": " على", "probability": 0.6865234375}, {"start": 1022.87, "end": 1023.33, "word": " نص", "probability": 0.83447265625}, {"start": 1023.33, "end": 1023.75, "word": " مل", "probability": 0.919189453125}, {"start": 1023.75, "end": 1023.97, "word": " من", "probability": 0.98779296875}, {"start": 1023.97, "end": 1024.13, "word": " الـ", "probability": 0.8271484375}, {"start": 1024.13, "end": 1024.39, "word": " Honey", "probability": 0.390869140625}, {"start": 1024.39, "end": 1024.83, "word": " Watch", "probability": 0.365966796875}, {"start": 1024.83, "end": 1025.05, "word": " من", "probability": 0.75390625}, {"start": 1025.05, "end": 1025.81, "word": " القاعدة", "probability": 0.99072265625}, {"start": 1025.81, "end": 1026.41, "word": " و", "probability": 0.431396484375}, {"start": 1026.41, "end": 1026.57, "word": " بعدين", "probability": 0.91650390625}, {"start": 1026.57, "end": 1026.95, "word": " بحطه", "probability": 0.9725341796875}, {"start": 1026.95, "end": 1027.05, "word": " في", "probability": 0.7900390625}, {"start": 1027.05, "end": 1027.23, "word": " الـ", "probability": 0.80908203125}, {"start": 1027.23, "end": 1027.45, "word": " Water", "probability": 0.5458984375}, {"start": 1027.45, "end": 1028.03, "word": " Bath", "probability": 0.6904296875}, {"start": 1028.03, "end": 1028.51, "word": " لمدة", "probability": 0.908447265625}, {"start": 1028.51, "end": 1028.91, "word": " 5", "probability": 0.6767578125}, {"start": 1028.91, "end": 1029.39, "word": " دقائق،", "probability": 0.6732666015625}, {"start": 1029.39, "end": 1029.77, "word": " لمدة", "probability": 0.9306640625}, {"start": 1029.77, "end": 1030.07, "word": " 5", "probability": 0.904296875}, {"start": 1030.07, "end": 1030.81, "word": " دقائق", "probability": 0.879638671875}, {"start": 1030.81, "end": 1031.43, "word": " إذا", "probability": 0.784912109375}, {"start": 1031.43, "end": 1031.81, "word": " ظهر", "probability": 0.8900146484375}, {"start": 1031.81, "end": 1032.03, "word": " بعد", "probability": 0.94189453125}, {"start": 1032.03, "end": 1032.19, "word": " ما", "probability": 0.677734375}, {"start": 1032.19, "end": 1032.45, "word": " طلع", "probability": 0.91357421875}, {"start": 1032.45, "end": 1032.59, "word": " من", "probability": 0.7724609375}, {"start": 1032.59, "end": 1032.75, "word": " الـ", "probability": 0.934326171875}, {"start": 1032.75, "end": 1032.95, "word": " Water", "probability": 0.90966796875}, {"start": 1032.95, "end": 1033.23, "word": " Bath", "probability": 0.93017578125}, {"start": 1033.23, "end": 1033.53, "word": " كان", "probability": 0.89453125}, {"start": 1033.53, "end": 1033.77, "word": " اللون", "probability": 0.80810546875}, {"start": 1033.77, "end": 1034.23, "word": " Red", "probability": 0.62744140625}, {"start": 1034.23, "end": 1035.05, "word": " إذا", "probability": 0.73583984375}, {"start": 1035.05, "end": 1035.47, "word": " هو", "probability": 0.60107421875}, {"start": 1035.47, "end": 1036.25, "word": " عبارة", "probability": 0.9945068359375}, {"start": 1036.25, "end": 1036.69, "word": " عن", "probability": 0.99658203125}, {"start": 1036.69, "end": 1037.65, "word": " Reducing", "probability": 0.7959798177083334}, {"start": 1037.65, "end": 1038.01, "word": " Sugar", "probability": 0.86669921875}, {"start": 1038.01, "end": 1038.77, "word": " إذا", "probability": 0.983154296875}, {"start": 1038.77, "end": 1039.43, "word": " اللون", "probability": 0.962646484375}, {"start": 1039.43, "end": 1039.57, "word": " ما", "probability": 0.85302734375}, {"start": 1039.57, "end": 1040.09, "word": " اتكونش", "probability": 0.803466796875}, {"start": 1040.09, "end": 1040.31, "word": " اللون", "probability": 0.96728515625}, {"start": 1040.31, "end": 1040.51, "word": " الـ", "probability": 0.698974609375}, {"start": 1040.51, "end": 1040.69, "word": " Red", "probability": 0.83447265625}, {"start": 1040.69, "end": 1040.93, "word": " إذا", "probability": 0.897216796875}, {"start": 1040.93, "end": 1041.35, "word": " هو", "probability": 0.94970703125}, {"start": 1041.35, "end": 1041.79, "word": " Non", "probability": 0.81689453125}, {"start": 1041.79, "end": 1042.73, "word": "-Reducing", "probability": 0.8126220703125}, {"start": 1042.73, "end": 1043.41, "word": " Sugar", "probability": 0.8408203125}, {"start": 1043.41, "end": 1044.05, "word": " بهيك", "probability": 0.57958984375}, {"start": 1044.05, "end": 1044.37, "word": " بنكون", "probability": 0.851806640625}, {"start": 1044.37, "end": 1045.05, "word": " خلصنا", "probability": 0.980224609375}, {"start": 1045.05, "end": 1045.71, "word": " تجارب", "probability": 0.991943359375}, {"start": 1045.71, "end": 1046.27, "word": " الفحص", "probability": 0.83544921875}, {"start": 1046.27, "end": 1046.41, "word": " و", "probability": 0.7568359375}, {"start": 1046.41, "end": 1046.81, "word": " الكشف", "probability": 0.9697265625}, {"start": 1046.81, "end": 1047.09, "word": " عن", "probability": 0.99658203125}, {"start": 1047.09, "end": 1047.91, "word": " السكريات", "probability": 0.9111328125}], "temperature": 1.0}, {"id": 48, "seek": 105842, "start": 1048.36, "end": 1058.42, "text": "مطلوب منكم تكتبولي ال principle لكل فحص على شكل معادلة زي ما عملنا في فحص qualitative test of amino acid", "tokens": [2304, 9566, 1211, 37746, 9154, 24793, 6055, 4117, 2655, 3555, 12610, 1829, 2423, 8665, 5296, 28820, 6156, 5016, 9381, 15844, 13412, 28820, 20449, 18513, 37977, 30767, 1829, 19446, 6225, 42213, 8315, 8978, 6156, 5016, 9381, 31312, 1500, 295, 24674, 8258], "avg_logprob": -0.15272485046851925, "compression_ratio": 1.203125, "no_speech_prob": 4.172325134277344e-07, "words": [{"start": 1048.36, "end": 1048.94, "word": "مطلوب", "probability": 0.83154296875}, {"start": 1048.94, "end": 1049.34, "word": " منكم", "probability": 0.956298828125}, {"start": 1049.34, "end": 1049.98, "word": " تكتبولي", "probability": 0.9296061197916666}, {"start": 1049.98, "end": 1050.1, "word": " ال", "probability": 0.953125}, {"start": 1050.1, "end": 1050.68, "word": " principle", "probability": 0.23095703125}, {"start": 1050.68, "end": 1051.3, "word": " لكل", "probability": 0.96484375}, {"start": 1051.3, "end": 1051.86, "word": " فحص", "probability": 0.9593098958333334}, {"start": 1051.86, "end": 1052.36, "word": " على", "probability": 0.845703125}, {"start": 1052.36, "end": 1052.8, "word": " شكل", "probability": 0.9833984375}, {"start": 1052.8, "end": 1053.64, "word": " معادلة", "probability": 0.9462890625}, {"start": 1053.64, "end": 1054.2, "word": " زي", "probability": 0.927490234375}, {"start": 1054.2, "end": 1054.34, "word": " ما", "probability": 0.98779296875}, {"start": 1054.34, "end": 1054.76, "word": " عملنا", "probability": 0.9453125}, {"start": 1054.76, "end": 1054.96, "word": " في", "probability": 0.90869140625}, {"start": 1054.96, "end": 1055.46, "word": " فحص", "probability": 0.9796549479166666}, {"start": 1055.46, "end": 1056.04, "word": " qualitative", "probability": 0.2066650390625}, {"start": 1056.04, "end": 1056.78, "word": " test", "probability": 0.82568359375}, {"start": 1056.78, "end": 1057.14, "word": " of", "probability": 0.96240234375}, {"start": 1057.14, "end": 1057.78, "word": " amino", "probability": 0.9013671875}, {"start": 1057.78, "end": 1058.42, "word": " acid", "probability": 0.744140625}], "temperature": 1.0}], "language": "ar", "language_probability": 1.0, "duration": 1060.6875625, "duration_after_vad": 1027.059718750002}
\ No newline at end of file
diff --git a/PL9fwy3NUQKwZ1Q7zOg1ObgufPMjRI3WY1/6BIvAPIvRuM_raw.srt b/PL9fwy3NUQKwZ1Q7zOg1ObgufPMjRI3WY1/6BIvAPIvRuM_raw.srt
new file mode 100644
index 0000000000000000000000000000000000000000..803fb077363dec2c6365320a511f86a1b3f98f0a
--- /dev/null
+++ b/PL9fwy3NUQKwZ1Q7zOg1ObgufPMjRI3WY1/6BIvAPIvRuM_raw.srt
@@ -0,0 +1,968 @@
+1
+00:00:00,000 --> 00:00:04,520
+هنا عندنا جدول بيقارن بين أنواع الـOxidizing agent
+
+2
+00:00:04,520 --> 00:00:08,460
+المستخدم نقارن بين الـBendix solution و الـFilling
+
+3
+00:00:08,460 --> 00:00:12,180
+solution و الـTolens reagent أولا يجي من ناحية
+
+4
+00:00:12,180 --> 00:00:16,640
+المكونات الـBendix بيتكون من كبر صلفات في ألكالاين
+
+5
+00:00:16,640 --> 00:00:21,060
+زيتريا دوّب في ألكالاين زيترا الفilling بيتكون من
+
+6
+00:00:21,060 --> 00:00:26,100
+كبر صلفات في ألكالاين ترتريا الـTolens بيتكون من
+
+7
+00:00:26,100 --> 00:00:30,530
+silver nitrate إن أكي و أسامانةطيب color of
+
+8
+00:00:30,530 --> 00:00:34,130
+solution إيش لون ال reagent بيكون؟ لون ال reagent
+
+9
+00:00:34,130 --> 00:00:37,610
+في حالة ال pendex بيكون دي بي بلو و في حالة ال
+
+10
+00:00:37,610 --> 00:00:41,290
+filling برضه دي بي بلو بيكون لونهم أزرق حكينا بسبب
+
+11
+00:00:41,290 --> 00:00:44,490
+وجود الكبر هو اللي بيعطي ال reagent اللون الأزرق
+
+12
+00:00:44,490 --> 00:00:49,390
+ال tulens بيكون colorless مالهوش لونطيب، بعد
+
+13
+00:00:49,390 --> 00:00:52,390
+التفاعل color after reaction with reducing sugar
+
+14
+00:00:52,390 --> 00:00:57,730
+بعد التفاعل الأكسد والاختزال، إيش هيصير اللون؟
+
+15
+00:00:57,730 --> 00:01:01,610
+الـBendix والـFilling بيتحول لونهم إلى break-red
+
+16
+00:01:01,610 --> 00:01:05,570
+depressive بتاعه اللي هو راسب لونه أحمر، طوبي،
+
+17
+00:01:05,570 --> 00:01:10,030
+ليتكون الكبار أكسان أما الـTolens، بيتحول إلى لون
+
+18
+00:01:10,030 --> 00:01:13,710
+silver mirror form اللي هو بيكون لون فضي وعمل زي
+
+19
+00:01:13,710 --> 00:01:19,690
+المرايا بسبب تكون الـAG الفندقالـ Species being
+
+20
+00:01:19,690 --> 00:01:23,590
+reduced مين هي المكونات اللي هيصير لها اختزال؟
+
+21
+00:01:23,590 --> 00:01:28,550
+السكر هو اللي هيختزل غيره، فالـ Oxidizing agent هو
+
+22
+00:01:28,550 --> 00:01:32,030
+اللي هيصير له اختزال، مين اللي هيصير له اختزال هو
+
+23
+00:01:32,030 --> 00:01:35,310
+الـ Oxidizing agentفي حالة الـ Bendect solution
+
+24
+00:01:35,310 --> 00:01:39,570
+الكبر هو اللي هيصير له اختزال هيتحول من Capric Ion
+
+25
+00:01:39,570 --> 00:01:43,190
+إلى Capric Ion ونفس الشيء بالنسبة للـ Filling هو
+
+26
+00:01:43,190 --> 00:01:47,170
+الـ Capric Ion هيتحول إلى Capric Ion في حالة الـ
+
+27
+00:01:47,170 --> 00:01:51,710
+Tollens هي الفضة هي اللي هتختزل من AG plus إلى AG
+
+28
+00:01:52,660 --> 00:01:56,600
+طيب، species being oxidized مين اللي هيصيرله
+
+29
+00:01:56,600 --> 00:02:00,520
+أكسدة؟ السكر هو اللي هيصيرله أكسدة في كل الحلقة
+
+30
+00:02:00,520 --> 00:02:04,300
+لأن هو بيختزل غيره و بيصيرله أكسدة فال reducing
+
+31
+00:02:04,300 --> 00:02:08,420
+sugar oxidized too حسب نوعه، هيتأكسد إما
+
+32
+00:02:08,420 --> 00:02:13,880
+لكربوكسيلات أو هيدروكسل كربوكسيلات في التلت حالات
+
+33
+00:02:13,880 --> 00:02:21,050
+اللي بيصيرله أكسدة هو السكرهنا لصورة يوتيوب نوعتش
+
+34
+00:02:21,050 --> 00:02:25,950
+باستخدام tool insert agent وبعد التفاعل كيف بيكون
+
+35
+00:02:25,950 --> 00:02:29,190
+اللون حاجة انها بيكون silver mirror phone اللي هو
+
+36
+00:02:29,190 --> 00:02:33,470
+لون فضي بس شكله زي المرايا هيك لامع فهذا هو نوعتش
+
+37
+00:02:33,470 --> 00:02:38,990
+كيف هيك شكلهفحصاتنا لليوم هي فحصات qualitative
+
+38
+00:02:38,990 --> 00:02:43,630
+test للكشف عن وجود السكريات تمام، هي فحصات
+
+39
+00:02:43,630 --> 00:02:47,950
+qualitative للكشف عن وجود الـ Carbohydrate أول فحص
+
+40
+00:02:47,950 --> 00:02:51,830
+نتعرف عليه هو الـ Bindect Test الـ Bindect، الـ
+
+41
+00:02:51,830 --> 00:02:54,790
+objective منه أو هو ولا إيش هذا الفحص to detect
+
+42
+00:02:54,790 --> 00:02:57,650
+the presence of reducing sugar distinguished
+
+43
+00:02:57,650 --> 00:03:01,470
+between reducing and non-reducing sugar وفحص خاص
+
+44
+00:03:01,470 --> 00:03:06,030
+للكشف عن ال reducing sugar عن السكريات المختزلة
+
+45
+00:03:06,920 --> 00:03:10,640
+فبيميز من الـ reducing و الـ non-reducing شغل مثلا
+
+46
+00:03:10,640 --> 00:03:13,360
+اعطيتك سكر و حكيتلك هل هذا reducing او non
+
+47
+00:03:13,360 --> 00:03:16,820
+-reducing كيف بدك تقرا فيه؟ بدك تشتغل فحص الـ
+
+48
+00:03:16,820 --> 00:03:20,480
+vindictive طيب على اي مبدأ بيشتغل الـ principle
+
+49
+00:03:20,480 --> 00:03:24,260
+بيشتغل على مبدأ ان هذا الـ reagent يحتوي على كبر
+
+50
+00:03:24,260 --> 00:03:29,200
+صلفات على كبر صلفات الكبر هذا الموجود في الكبر
+
+51
+00:03:29,200 --> 00:03:34,920
+صلفات يعمل ك oxidizing agent بيروح بي acid السكر
+
+52
+00:03:35,220 --> 00:03:42,620
+والسكر بيشتغل ك reducing agent بيعمل اختزال للسكر
+
+53
+00:03:42,620 --> 00:03:47,340
+فالـ principle كيف هتكون على شكل المعادلات زي ما
+
+54
+00:03:47,340 --> 00:03:50,920
+أخدنا في المعمل الأول لو بدنا نعملها أنه عندي سكر
+
+55
+00:03:50,920 --> 00:03:54,120
+على فرض أنه aldehyde هو إما هيكون aldehyde أو
+
+56
+00:03:54,120 --> 00:03:57,720
+كيتون نعمل معادلة للـ aldehyde لحال ومعادلة
+
+57
+00:03:57,720 --> 00:04:03,440
+للكيتون لحال هو aldehydeزائد الكبركايون اللى موجود
+
+58
+00:04:03,440 --> 00:04:08,420
+فى الكبرسالفات فى وجود alkaline media alkaline pH
+
+59
+00:04:08,420 --> 00:04:12,740
+بيعطينا النتج الالتهلالدهايت هيصير له oxidation
+
+60
+00:04:12,740 --> 00:04:17,720
+تحول إلى كربوكسيلات طب والكبركايون هيصير له
+
+61
+00:04:17,720 --> 00:04:22,640
+reduction إلى كبرس اوكسايد بترسب على شكل كبرس
+
+62
+00:04:22,640 --> 00:04:28,680
+اوكسايدلونه brick red أحمر طوم فإذا الناتج أعطاني
+
+63
+00:04:28,680 --> 00:04:32,380
+لون اتحول اللون إلى brick red إذا هذا reducing
+
+64
+00:04:32,380 --> 00:04:37,480
+sugar إذا ماعطانيش إذا non-reducing sugar لو بدك
+
+65
+00:04:37,480 --> 00:04:42,410
+تبم عادلة تانية للكيتون، الكيتون ماءفي وزاقد
+
+66
+00:04:42,410 --> 00:04:46,810
+كبرسالفات اللي هو الـ Capric Ion في وجود alkaline
+
+67
+00:04:46,810 --> 00:04:50,510
+media الكيتون بدي يصير له oxidation بس بدي يتحول
+
+68
+00:04:50,510 --> 00:04:54,910
+إلى هيدروكس الكربوكسيلان طيب، والـ Capric Ion
+
+69
+00:04:54,910 --> 00:04:59,070
+بيترسب، بيصير له reduction، بترسب على شكل Caprous
+
+70
+00:04:59,070 --> 00:05:05,830
+Oxideلونه بريك ريب بريسبيتات بيحكيلي the color of
+
+71
+00:05:05,830 --> 00:05:10,130
+بريسبيتات بتعتمد شدة اللون بتعتمد على تركيز
+
+72
+00:05:10,130 --> 00:05:16,610
+reducing sugar كدهش في كمية من هذا السكةخطوات
+
+73
+00:05:16,610 --> 00:05:20,650
+إجراء التجربة هاي أني بحط واحد من من السكر اللي
+
+74
+00:05:20,650 --> 00:05:23,750
+موجود عندى مثلا عندي عدة سكريات سكر جلكوز،
+
+75
+00:05:23,750 --> 00:05:27,810
+فراكتوز، سكروز، لاكتوز، مالتوز، ودول بدكشف عنهم،
+
+76
+00:05:27,810 --> 00:05:31,310
+كل واحد بشتغلوا بتيوم بحط واحد من من هذا السكر
+
+77
+00:05:31,310 --> 00:05:34,930
+واضيف عليه واحد من من الـBindex Reagent من
+
+78
+00:05:34,930 --> 00:05:39,310
+الـReagent وبحطهم في الـwater bath لمدة خمس ديالي
+
+79
+00:05:39,880 --> 00:05:43,040
+بعدين بطلع إذا اتحول اللون إلى brick red إلى لون
+
+80
+00:05:43,040 --> 00:05:47,240
+أحمر إذا هذا reducing sugar إذا ما اتغيرش اللون
+
+81
+00:05:47,240 --> 00:05:52,020
+إذا هو non-reducing sugar أو إنه طلع لون تاني غير
+
+82
+00:05:52,020 --> 00:05:57,840
+ال brick red هو non-reducing sugarالفحص التاني
+
+83
+00:05:57,840 --> 00:06:02,760
+اسمه Barfeuil Test Barfeuil Test هذا إيش ال
+
+84
+00:06:02,760 --> 00:06:06,080
+objective منه؟ to distinguish between mono and
+
+85
+00:06:06,080 --> 00:06:09,960
+disaccharide بيفرق من السكريات الأحدية و الثنائية
+
+86
+00:06:09,960 --> 00:06:12,800
+يعني لو عندي سكر مش عارف هل هو mono ولا
+
+87
+00:06:12,800 --> 00:06:16,380
+disaccharide بستخدم فحص ال Barfeuil Test عشان
+
+88
+00:06:16,380 --> 00:06:20,060
+أفرقه هل هو mono ولا disaccharideكيف الـ principle
+
+89
+00:06:20,060 --> 00:06:25,320
+طريقة شغله الـ principle إنه الـ Parfaits reagent
+
+90
+00:06:25,320 --> 00:06:30,750
+اللي أنا بستخدمه بيتكون main coverage أيهطبعا عند
+
+91
+00:06:30,750 --> 00:06:34,850
+الـ Reagent بيحتوي على Capric Ion فأنا بحط السكر،
+
+92
+00:06:34,850 --> 00:06:38,370
+السكر هذا النوعيا هيكون Aldehyde يا كيتو مثلا
+
+93
+00:06:38,370 --> 00:06:41,890
+Aldehyde، نيني نكتب معادلة Aldehyde الـ Aldehyde
+
+94
+00:06:41,890 --> 00:06:44,730
+زائد الـ Capric Ion اللي موجود في الـ Reagent
+
+95
+00:06:44,730 --> 00:06:48,470
+بيتحول، بيطلع عندي النتج الـ Aldehyde بتحول
+
+96
+00:06:48,470 --> 00:06:54,940
+لكربوكسيلات و Capric Oxideأما إذا كان كيتون كيتون
+
+97
+00:06:54,940 --> 00:06:57,400
+باضيف عليه الـ reagent اللي هو الـ Capric Ion
+
+98
+00:06:57,400 --> 00:07:02,040
+بتحول الكيتون إلى Hydroxyl Carboxylate والـ Capric
+
+99
+00:07:02,040 --> 00:07:06,900
+Ion بتحول إلى Caprous Oxideاللون في الأخر في
+
+100
+00:07:06,900 --> 00:07:11,280
+المعادلتين اللون بيكون break red إذا اتحول إلى لون
+
+101
+00:07:11,280 --> 00:07:16,440
+break red بيكون هذا monosaccharide بيحكي في الـ
+
+102
+00:07:16,440 --> 00:07:18,900
+Parvoid reagent react with monosaccharide to
+
+103
+00:07:18,900 --> 00:07:21,940
+produce caprous oxide faster rate than
+
+104
+00:07:21,940 --> 00:07:25,120
+disaccharide ال Parvoid reagent بيتفاعل مع
+
+105
+00:07:25,120 --> 00:07:27,160
+الإتنين، مع الـ monosaccharide و مع الـ
+
+106
+00:07:27,160 --> 00:07:30,830
+disaccharideبيعطيني لون ولكن تفاعله مع الـ
+
+107
+00:07:30,830 --> 00:07:34,150
+monosaccharide أسرع من تفاعله مع الـ disaccharide
+
+108
+00:07:34,150 --> 00:07:38,590
+فاحنا لما نشتغل نحطه وقت معين لواجهة هذا بيسمح
+
+109
+00:07:38,590 --> 00:07:42,650
+بتفاعل فقط الـ monosaccharide لو أتأخرنا عليه ممكن
+
+110
+00:07:42,650 --> 00:07:46,010
+الـ disaccharide يتفاعل كمان فاحنا بدنا نحطه بس
+
+111
+00:07:46,010 --> 00:07:50,270
+لواجهة تفاعل الـ monosaccharide و بعد هيك بطلا إذا
+
+112
+00:07:50,270 --> 00:07:55,360
+تغيري اللون يعني هذا monosaccharideالـ principle
+
+113
+00:07:55,360 --> 00:08:00,580
+للبرفويدز زي الـ بين dicts ولكن بدون وجود وسط
+
+114
+00:08:00,580 --> 00:08:07,300
+قاعد، لا تحتاج إلى وجود وسط قاعدكيف طريقة العمل؟
+
+115
+00:08:07,300 --> 00:08:10,880
+احط واحد مل من السكر اللى موجود عندى على واحد مل
+
+116
+00:08:10,880 --> 00:08:15,040
+من الاجان ثم بحطه في ال water bath لمدة عشر دقائق
+
+117
+00:08:15,040 --> 00:08:18,060
+بطلع من ال water bath اللى اتكون عندى red
+
+118
+00:08:18,060 --> 00:08:21,540
+precipitate اللون الأحمر، الراسب اللون الأحمر، إذا
+
+119
+00:08:21,540 --> 00:08:25,600
+ده mono-saccharide إذا ما اتكونش، إذا هو dye
+
+120
+00:08:25,600 --> 00:08:31,990
+-saccharide أو سكر آخرفحص التالت هو فحص ال bios أو
+
+121
+00:08:31,990 --> 00:08:37,030
+اسمه تاني orsenol test bios or orsenol هذا خاص
+
+122
+00:08:37,030 --> 00:08:40,550
+للكشف عن ال pentose objective for the detection of
+
+123
+00:08:40,550 --> 00:08:43,710
+pentose it distinguishes between pentose and
+
+124
+00:08:43,710 --> 00:08:48,750
+hexose بيفرق بين الخماسي والسداسي هو للكشف عن ال
+
+125
+00:08:48,750 --> 00:08:53,570
+pentose عن الخماسي فبيفرق بين الخماسي والسداسي كيف
+
+126
+00:08:53,570 --> 00:08:58,010
+ال principle أنا بستخدم bios reagent bios reagent
+
+127
+00:08:58,340 --> 00:09:01,380
+اللي هو شو مكوناته هذا الـ Biles Region واحد من
+
+128
+00:09:01,380 --> 00:09:06,000
+عشرة في المية أورسينول Concentrated HCl واحد من
+
+129
+00:09:06,000 --> 00:09:11,930
+عشرة في المية فرق الكلورايسيت H2Oإن هاد الـ
+
+130
+00:09:11,930 --> 00:09:16,150
+Concentrated HCl بيعمل الـ dehydration للسُكّار
+
+131
+00:09:16,150 --> 00:09:20,230
+قلت الـ Biles بيفرق بين الـ Pentose و الـ Hexose
+
+132
+00:09:20,230 --> 00:09:24,010
+كيف بدي اتفعل الـ Principle بحط بيلز بحط السُكّار
+
+133
+00:09:24,010 --> 00:09:28,310
+أنا مثلا Pentose و بحط عليه الـ Biles reagent
+
+134
+00:09:28,310 --> 00:09:33,130
+المكونات التلاتة طبعا كلهم مخلطين بيتحول السُكّار
+
+135
+00:09:33,130 --> 00:09:37,990
+إلى حاجة اسمها Fair For Allفيرفورال و اورسينول
+
+136
+00:09:37,990 --> 00:09:42,590
+اللي ضال عندنا بتحول السكر إلى فيرفورال فيرفورال
+
+137
+00:09:42,590 --> 00:09:47,650
+ماء اورسينول في وجود فرك ايون من الفرك كلورايت جاي
+
+138
+00:09:47,650 --> 00:09:55,030
+بتكون عندى لون blue to green لون أخضر مزرقأو
+
+139
+00:09:55,030 --> 00:09:58,910
+precipitate على شكل هذا اللون اللي هو أخضر مزرق
+
+140
+00:09:58,910 --> 00:10:02,610
+بيطلع عندي إذا طلع هذا اللون إذا الموجود بنتاز
+
+141
+00:10:02,610 --> 00:10:06,490
+بيحكي لي إذا طولت عليه شهو يسار اللون براون بتكون
+
+142
+00:10:06,490 --> 00:10:10,370
+لون براون precipitate لون راسف لونه بني بيكون
+
+143
+00:10:10,370 --> 00:10:16,240
+الموجود hexoseفلو بتدخل دلخص معادلة الـ Biles على
+
+144
+00:10:16,240 --> 00:10:19,720
+شكل معادلة، دلخص الـ principle على شكل معادلة، انه
+
+145
+00:10:19,720 --> 00:10:21,740
+الـ Pentose، هذا الفحص كان للـ Pentose، الـ
+
+146
+00:10:21,740 --> 00:10:26,240
+Pentose في وجود HCl، أول حاجة بتشتغل من الريجط
+
+147
+00:10:26,240 --> 00:10:30,200
+اللي هو الـ HCl، بيعمل dehydration للسجر، فبينتج
+
+148
+00:10:30,200 --> 00:10:35,620
+عنه، بيتحول إلى Ferfolan زاد Orsinol زاد Ferric
+
+149
+00:10:35,620 --> 00:10:40,740
+Chlorideأذا أنا عند الـ Pentose في وجود HCl
+
+150
+00:10:40,740 --> 00:10:46,620
+بيعطيني Fervorol زي اورسينول زي تفرج الجلورال هدول
+
+151
+00:10:46,620 --> 00:10:50,100
+التلاتة مع بعض هاليات بيتفعلوا بيعطوني Complex
+
+152
+00:10:50,100 --> 00:10:57,060
+لونه blue to green Complex لونه blue to greenكيف
+
+153
+00:10:57,060 --> 00:11:02,320
+خطوات العمل بحط واحد مل من السكر على واحد و نص مل
+
+154
+00:11:02,320 --> 00:11:07,320
+من ال bios reagent وبعملهم خلط mix واحد مل من
+
+155
+00:11:07,320 --> 00:11:11,020
+السكر على واحد و نص مل من ال reagent بعد هيك
+
+156
+00:11:11,020 --> 00:11:14,180
+بسخنها على اللهب مش في ال water bath هي على اللهب
+
+157
+00:11:14,180 --> 00:11:18,420
+بسخنها لحد ما تغلي بعد ما تغلي بحطها بالراكد تبرد
+
+158
+00:11:18,420 --> 00:11:21,960
+إذا اتحول اللون إلى blue to green is positive إذا
+
+159
+00:11:21,960 --> 00:11:25,540
+السكر اللي موجود عندى بنتوتز إذا ماتحولش إذا
+
+160
+00:11:25,540 --> 00:11:31,050
+negativeالفحص اللي بعد هي اسمه Silvanov او RIS
+
+161
+00:11:31,050 --> 00:11:35,790
+Arsenal Test Silvanov او RIS Arsenal Test ايش
+
+162
+00:11:35,790 --> 00:11:39,110
+الهدف من هذا الفحص for detection of ketosis يكشف
+
+163
+00:11:39,110 --> 00:11:42,930
+عن الـ ketosis السكر اللي يحتوي على كيتون اجرم
+
+164
+00:11:44,450 --> 00:11:48,850
+البرنسيبل سيلوانوف تيست two distinguish between
+
+165
+00:11:48,850 --> 00:11:52,290
+hexose with a ketone group and hexose with
+
+166
+00:11:52,290 --> 00:11:55,610
+aldehyde group هو الكشف بيفرق بين اللي عنده
+
+167
+00:11:55,610 --> 00:11:59,810
+aldehyde عن اللي عنده كيتون ويكونه hexose يكون
+
+168
+00:11:59,810 --> 00:12:06,070
+سداثيهذا الفحص لو اشتغلتي مع كيتوز، مع كيتوز اللي
+
+169
+00:12:06,070 --> 00:12:10,070
+عنده كيتون جروب، بيعطيكي لون Red، هيعطيكي لون أحمر
+
+170
+00:12:10,070 --> 00:12:13,390
+وبيتكون بسرعة، it's formed rapidly، إذا اتكون لون
+
+171
+00:12:13,390 --> 00:12:17,590
+أحمر بسرعة، إذا اللي موجود كيتون، طيب الالدوز
+
+172
+00:12:17,590 --> 00:12:21,410
+بتعطيني light pink color و بتاخد واجهة أسرع، واجهة
+
+173
+00:12:21,410 --> 00:12:26,510
+أطول، longer timeإذا اتكون عندي اللون البنك وبواجه
+
+174
+00:12:26,510 --> 00:12:29,790
+ا طويل لما اتكون إذا اللي موجود عندي الـdose
+
+175
+00:12:29,790 --> 00:12:36,170
+الـketohexose يعني هو سداسي وعنده ketone group زي
+
+176
+00:12:36,170 --> 00:12:42,370
+الـfructose وعنا فيه حاجة di-saccharide وأحد
+
+177
+00:12:42,370 --> 00:12:45,870
+مكوناته يكون الـketohexose فجيت الـdi-saccharide
+
+178
+00:12:45,870 --> 00:12:49,410
+زي السكروز هو عبارة عن انتحار جليكوز مع
+
+179
+00:12:49,410 --> 00:12:53,860
+الـfructoseالـ fractose يعتبر ketohexose فأحد
+
+180
+00:12:53,860 --> 00:12:59,840
+مكونات السكاروز هو fractose وهو ketohexose يعني
+
+181
+00:12:59,840 --> 00:13:04,160
+هيعطيني positive مع هذا الفحص ولكن بدرجة أخف لللون
+
+182
+00:13:04,160 --> 00:13:09,660
+هيعطيني لون cherry red أحمر كرزي أما السكريات
+
+183
+00:13:09,660 --> 00:13:12,280
+الأخرى اللي هي الـdose اللي عندهاش ketone group
+
+184
+00:13:12,280 --> 00:13:16,500
+عندها aldehyde group تعطيني لون من yellow to pink
+
+185
+00:13:16,500 --> 00:13:22,920
+from yellow to pinkالـ principle مبتدأ العمل أنه
+
+186
+00:13:22,920 --> 00:13:26,080
+في عندي reaction تسمى الـ cellulose reaction اللي
+
+187
+00:13:26,080 --> 00:13:29,620
+هو عبارة عن خمسة من عشرة في المية risk of arsenol
+
+188
+00:13:29,620 --> 00:13:34,140
+في تلاتة normal HCl مجرد ما أضيف هذا ال reaction
+
+189
+00:13:34,140 --> 00:13:39,540
+بتيجي الـ concentrated HCl أولا شىء هذا الفحص للـ
+
+190
+00:13:39,540 --> 00:13:43,520
+ketose فعندي ketohexose الـ ketohexose في وجود الـ
+
+191
+00:13:43,520 --> 00:13:47,280
+HCl اللي موجودة في ال reaction بتعمل dehydration
+
+192
+00:13:47,280 --> 00:13:51,890
+للسكربتحولوا إلى مشتقات الفيرفورال اللي هي
+
+193
+00:13:51,890 --> 00:13:56,570
+Hydroxymethylferforate Hydroxy بتحول الـ Ketose
+
+194
+00:13:56,570 --> 00:14:01,950
+إلى Hydroxymethylferforate في وجود الـ HCl فالجاتب
+
+195
+00:14:01,950 --> 00:14:05,110
+بيجي الـ Hydroxymethylferforate بيتفاعل مع الـ
+
+196
+00:14:05,110 --> 00:14:09,250
+Resorcinol اللي في الرياجان بيعطيني Complex لونه
+
+197
+00:14:09,250 --> 00:14:15,950
+Cherry Red لون أحمر كرزي أو لون Redوهذا التفاعل
+
+198
+00:14:15,950 --> 00:14:19,190
+بيشتغل بسرعة خلال two minutes within two minutes
+
+199
+00:14:19,190 --> 00:14:23,710
+أما الالدوهيكسوز بتتفاعل بشكل بطيء مع هذا وبتكون
+
+200
+00:14:23,710 --> 00:14:27,470
+نفس الناتج ولكن بيكون أبطأ إذا ال principle لو
+
+201
+00:14:27,470 --> 00:14:32,610
+بتعملها على شكل معادلة بحكي كيتوهيكسوز في وجود HCl
+
+202
+00:14:32,610 --> 00:14:37,170
+بيعطيني Hydroxyl Methyl Ferforyl بيتفاعل مع
+
+203
+00:14:37,170 --> 00:14:43,450
+الرسورسينول بيعطيني Complex Lonoredكيف خطوات إجراء
+
+204
+00:14:43,450 --> 00:14:47,970
+التجربة؟ بحط تلاتة مل من الاجن على drop واحدة من
+
+205
+00:14:47,970 --> 00:14:52,010
+السكة بعدين بحطه في ال water bath لمدة تلاتة مل
+
+206
+00:14:52,010 --> 00:14:55,670
+بعدين بقلق إذا اتكون عندى اللون ال red إذا هذا
+
+207
+00:14:55,670 --> 00:15:00,470
+positive وهذا عبارة عن كيتوز إذا ما اتكونش إذا
+
+208
+00:15:00,470 --> 00:15:06,030
+negativeآخر فحص هو الـ Bicric Acid Test فحص الـ
+
+209
+00:15:06,030 --> 00:15:09,990
+Bicric Acid الـ objective منه يستخدم للكشف عن الـ
+
+210
+00:15:09,990 --> 00:15:13,810
+reducing sugar يعني زيه زي أول فحص اللي هو الـ
+
+211
+00:15:13,810 --> 00:15:18,390
+Vindict Test للكشف عن الـ reducing sugar زي أول
+
+212
+00:15:18,390 --> 00:15:22,670
+فحص اللي هو الـ Vindict Test كيف ال principle أنه
+
+213
+00:15:22,670 --> 00:15:26,470
+عندي Bicric Acid اللي هو اسمه تاني اتنين اربع ستة
+
+214
+00:15:26,470 --> 00:15:30,990
+Trinitrophenol Bicric Acid هو اتنين اربع ستة
+
+215
+00:15:30,990 --> 00:15:35,680
+Trinitrophenolأو الـ TMP بيجي هذا البكريك أسد
+
+216
+00:15:35,680 --> 00:15:40,260
+بيتفعل مع الـ Reducing Sugar وبيصير لو واحد فيهم
+
+217
+00:15:40,260 --> 00:15:44,780
+أكسدة وواحد فيهم اختزال وبيتكون عندي الـ Red Color
+
+218
+00:15:44,780 --> 00:15:50,760
+جاي من وين هذا الـ Red Color من عملية اختزال
+
+219
+00:15:50,760 --> 00:15:57,500
+البكريك أسد إلى Sodium Bicaramideالـ Red color زي
+
+220
+00:15:57,500 --> 00:16:00,960
+من حدوث reduction للـ Bacric Acid لأنه بيصيبله
+
+221
+00:16:00,960 --> 00:16:04,980
+اختزان، بتحول لـ Sodium Bicaramide فلو بدك تكتب
+
+222
+00:16:04,980 --> 00:16:08,400
+معادلة للـ Principle للـ Bacric Acid أن عندي
+
+223
+00:16:08,400 --> 00:16:13,660
+Aldehyde زائد Bacric Acid في وجود أنيوات، في وجود
+
+224
+00:16:13,660 --> 00:16:19,270
+قاعدةالـ aldehyde بيصير له oxidation بيتأكسد إلى
+
+225
+00:16:19,270 --> 00:16:22,790
+حاجة اسمها الـ carboxylate حكينا الـ bicaric acid
+
+226
+00:16:22,790 --> 00:16:26,390
+بيصير له reduction إلى أيش؟ إلى Sodium Bicaramate
+
+227
+00:16:26,390 --> 00:16:30,010
+Sodium Bicaramate هذا له نورين، طب إذا كيتون مش
+
+228
+00:16:30,010 --> 00:16:34,380
+aldehyde؟الكيتون زاد الـbicaric acid في وجود
+
+229
+00:16:34,380 --> 00:16:38,280
+انيواتش الكيتون إيش هيصير له oxidation إلى
+
+230
+00:16:38,280 --> 00:16:43,020
+هيدروكسلكربوكسيلات والبيكريك أسن بيصير له
+
+231
+00:16:43,020 --> 00:16:47,800
+reduction إلى صوديوم بكرامات لونه red إذا اتكون
+
+232
+00:16:47,800 --> 00:16:51,820
+اللون ال red إذا هو reducing sugar إذا ما اتكونش
+
+233
+00:16:51,820 --> 00:16:58,080
+إذا هو non-reducing sugar كيف خطوات العمل بحط واحد
+
+234
+00:16:58,080 --> 00:17:03,750
+مل من السكرعلى 1 مل من الـ Bacric Acid على نص مل
+
+235
+00:17:03,750 --> 00:17:07,230
+من الـ Honey Watch من القاعدة و بعدين بحطه في الـ
+
+236
+00:17:07,230 --> 00:17:12,030
+Water Bath لمدة 5 دقائق، لمدة 5 دقائق إذا ظهر بعد
+
+237
+00:17:12,030 --> 00:17:15,470
+ما طلع من الـ Water Bath كان اللون Red إذا هو
+
+238
+00:17:15,470 --> 00:17:20,310
+عبارة عن Reducing Sugar إذا اللون ما اتكونش اللون
+
+239
+00:17:20,310 --> 00:17:25,050
+الـ Red إذا هو Non-Reducing Sugar بهيك بنكون خلصنا
+
+240
+00:17:25,050 --> 00:17:29,980
+تجارب الفحص و الكشف عن السكرياتمطلوب منكم تكتبولي
+
+241
+00:17:29,980 --> 00:17:34,960
+ال principle لكل فحص على شكل معادلة زي ما عملنا في
+
+242
+00:17:34,960 --> 00:17:38,420
+فحص qualitative test of amino acid
+
diff --git a/PL9fwy3NUQKwZ1Q7zOg1ObgufPMjRI3WY1/9CmoB4eQs2I.srt b/PL9fwy3NUQKwZ1Q7zOg1ObgufPMjRI3WY1/9CmoB4eQs2I.srt
new file mode 100644
index 0000000000000000000000000000000000000000..72c1ba49d5da861ae8b1fc2c71d08eb8b08ba5b4
--- /dev/null
+++ b/PL9fwy3NUQKwZ1Q7zOg1ObgufPMjRI3WY1/9CmoB4eQs2I.srt
@@ -0,0 +1,1338 @@
+1
+00:00:00,000 --> 00:00:05,280
+كيف موضوع جديد هو isolation of cholesterol from
+
+2
+00:00:05,280 --> 00:00:11,100
+egg yolk عزل الكوليسترول من صفار البيضة، نجيب صفار
+
+3
+00:00:11,100 --> 00:00:16,450
+بيضة و نعزل منها الكوليسترول، بحكي اليوم الحاجة أن 
+
+4
+00:00:16,450 --> 00:00:19,970
+أتعرف على الكوليسترول، الكوليسترول هو most abundant
+
+5
+00:00:19,970 --> 00:00:25,290
+steroid in the body هو أشهر الكوليسترول هو أشهر
+
+6
+00:00:25,290 --> 00:00:29,870
+steroid، أشهر lipid موجود في الجسم، أشهر lipid موجود
+
+7
+00:00:29,870 --> 00:00:35,110
+في الجسم هو الكوليسترول، is a waxy substance found in
+
+8
+00:00:35,110 --> 00:00:40,690
+all of the body cells هو عبارة عن مادة شمعية قوامها
+
+9
+00:00:40,690 --> 00:00:47,020
+شمعي موجود في كل أجزاء الجسم، كيف تركيبه؟ تركيبه 
+
+10
+00:00:47,020 --> 00:00:53,040
+عبارة عن Methyl group اللي هي CH3 مع Alkyl Chain
+
+11
+00:00:53,040 --> 00:00:59,240
+اللي هي سلسلة مرتبط فيها CH3 و OH و Steroid
+
+12
+00:00:59,240 --> 00:01:02,860
+nucleus، Steroid nucleus هو عبارة عن الأربع حلقات
+
+13
+00:01:02,860 --> 00:01:08,480
+اللي تحت، متصل فيهم OH و سلسلة بنسميها Alkyl Chain و
+
+14
+00:01:08,480 --> 00:01:13,940
+CH3 مرتبطة في هذه السلسلة، هذه عبارة عن تركيبة 
+
+15
+00:01:13,940 --> 00:01:20,050
+الكوليسترول، الـ Structure A، إيش وظائف الكوليسترول، ال
+
+16
+00:01:20,050 --> 00:01:23,310
+function؟ أول وظيفة help and maintain cell
+
+17
+00:01:23,310 --> 00:01:28,230
+membrane، يعمل على الحفاظ و حماية ال cell membrane
+
+18
+00:01:28,230 --> 00:01:33,230
+بيحمي و يحافظ على ال cell membrane، وظيفة ثانية 
+
+19
+00:01:33,230 --> 00:01:37,890
+protect and insulate nerve fiber، بيعمل على حماية و
+
+20
+00:01:37,890 --> 00:01:43,910
+عزل الألياف العصبية، حماية و عزل الألياف العصبية
+
+21
+00:01:45,300 --> 00:01:49,020
+ثلاثة، involved in the formation of sex hormones زي
+
+22
+00:01:49,020 --> 00:01:54,040
+الاستروجين، يدخل في تركيب ال sex hormones، الهرمونات
+
+23
+00:01:54,040 --> 00:01:58,620
+الجنسية زي الاستروجين، بيدخل في تركيبها الكوليسترول
+
+24
+00:01:58,620 --> 00:02:02,800
+أربعة، production of bile salt which help digesting
+
+25
+00:02:02,800 --> 00:02:07,100
+fat، زي ما حكينا المعمل اللي فات، هو يدخل في تصنيع
+
+26
+00:02:07,100 --> 00:02:10,900
+الـ bile salt، اللي هي أملاح العصارة الصفراوية التي 
+
+27
+00:02:10,900 --> 00:02:17,840
+تساعد على هضم الدهون، حكينا عنها المعمل اللي فات، كيف 
+
+28
+00:02:17,840 --> 00:02:21,240
+الكوليسترول موجود في الجسم؟ هلجيب الكوليسترول من 
+
+29
+00:02:21,240 --> 00:02:25,960
+أين يحصل عليه الجسم؟ 80% من الكوليسترول بتصنع في
+
+30
+00:02:25,960 --> 00:02:31,460
+الـ liver، synthesized in the liver بالنسبة 80% و 20% 
+
+31
+00:02:31,460 --> 00:02:36,220
+بناخدها من الواجبات، موجودة في الحليب، في البيض، في
+
+32
+00:02:36,220 --> 00:02:43,280
+اللحمة، 20%، أما 80% بيصنع الجسم في الـ liver، بيحتاج
+
+33
+00:02:43,280 --> 00:02:46,480
+الجسم للكوليسترول، بناءً على وضعه، بيحتاج للـ cell
+
+34
+00:02:46,480 --> 00:02:49,480
+membrane، لحماية ال cell membrane، لعزل ال nerve
+
+35
+00:02:49,480 --> 00:02:53,720
+tissue، عزل الألياف العصبية، في تصنيع ال steroid
+
+36
+00:02:53,720 --> 00:02:57,960
+hormone، في تصنيع vitamin D، هدول كل استخدامات 
+
+37
+00:02:57,960 --> 00:02:59,120
+للكوليسترول
+
+38
+00:03:01,080 --> 00:03:05,520
+الكوليسترول النسبة الطبيعية له في الجسم أن يكون أقل
+
+39
+00:03:05,520 --> 00:03:10,240
+من 200 ميلي جرام per deciliter، كوليسترول النسبة 
+
+40
+00:03:10,240 --> 00:03:14,600
+الطبيعية له في الجسم أن يكون أقل من 200 ميلي جرام 
+
+41
+00:03:14,600 --> 00:03:20,400
+per deciliter، إذا زاد، elevated plasma cholesterol
+
+42
+00:03:20,400 --> 00:03:23,820
+exceeds 200 ميلي جرام per deciliter، زاد عن 200
+
+43
+00:03:23,820 --> 00:03:26,940
+ميلي جرام per deciliter، بنسمي هذه الحالة
+
+44
+00:03:26,940 --> 00:03:31,410
+كوليستيرولِيميا، الـ Cholestrolimia، معناها ارتفاع 
+
+45
+00:03:31,410 --> 00:03:36,350
+الكوليسترول أكتر من 200 ميلي جرام per deciliter
+
+46
+00:03:36,350 --> 00:03:40,170
+وهذا إذا ارتفع، بيصير  Artery clots، arteries، و يهاجم 
+
+47
+00:03:40,170 --> 00:03:44,950
+level 4، بليتليت، بيصير انسداد في الشرايين و الأوردة
+
+48
+00:03:44,950 --> 00:03:46,730
+و ممكن تسبب جلطات
+
+49
+00:03:50,000 --> 00:03:53,240
+أنا عندي عبارة عن جدول بيوضح الأطعمة اللي بتحتوي على
+
+50
+00:03:53,240 --> 00:03:57,280
+الكوليسترول، مش مهمة كتير، هي موجودة في الكبدة،
+
+51
+00:03:57,280 --> 00:04:00,800
+موجودة في البيض، موجودة في سرطان البحر، في الدجاج
+
+52
+00:04:00,800 --> 00:04:04,640
+المقلي، في الهامبورجر، في الحليب، في المارجرينة،
+
+53
+00:04:04,640 --> 00:04:07,600
+موجودة في أشياء كتير، موجودة كوليسترول، لكن نسبة 
+
+54
+00:04:07,600 --> 00:04:14,340
+اللي بنحصل عليها من الجسم هي فقط 20%، في عندنا حاجة
+
+55
+00:04:14,340 --> 00:04:19,130
+اسمها lipoprotein، الـ lipoprotein، إيش هي؟ هلجيب
+
+56
+00:04:19,130 --> 00:04:23,790
+اللبد هو non-polar، بقدرش يدوب في الماء، فكيف بده
+
+57
+00:04:23,790 --> 00:04:27,670
+يمشي في الجسم هذا اللبد؟ هلجيب اللبد لأنه non
+
+58
+00:04:27,670 --> 00:04:32,510
+-polar، بيحتاج إلى نواقل تنقله في الجسم، النواقل هذه 
+
+59
+00:04:32,510 --> 00:04:37,730
+عبارة عن lipoprotein، الـ lipoprotein هي نواقل اللبد
+
+60
+00:04:37,730 --> 00:04:43,150
+هي نواقل لنقل اللبد، لأنه اللبد بقدرش يتحرك لأنه 
+
+61
+00:04:43,150 --> 00:04:48,580
+non-polar، الليبوبروتين هي عبارة عن لبد مع بروتين، و
+
+62
+00:04:48,580 --> 00:04:53,480
+عليها كمان فوسفوليبيد، الليبوبروتين هي عبارة عن لبد 
+
+63
+00:04:53,480 --> 00:04:58,040
+مع بروتين و فوسفوليبيد، اللي جاب اللبد لما يتصل على 
+
+64
+00:04:58,040 --> 00:05:02,580
+سطحه بروتين و فوسفوليبيد، بتعطيه خاصية أنه يصير
+
+65
+00:05:02,580 --> 00:05:06,640
+soluble in water، لأن الـ surface بيكون عبارة عن 
+
+66
+00:05:06,640 --> 00:05:10,560
+polar lipid، بتكون الخارج السطح المحاط في هذا اللبد 
+
+67
+00:05:10,560 --> 00:05:14,250
+هو عبارة عن مواد polar اللي هي ال phospholipid، ال
+
+68
+00:05:14,250 --> 00:05:18,870
+phosphatic group، بتكون على السطح فبيكون كأنه polar
+
+69
+00:05:18,870 --> 00:05:23,550
+بيكون soluble in water، ليش؟ لإتصاله بمجموعة خلتة
+
+70
+00:05:23,550 --> 00:05:26,990
+polar زي ال phospholipid، اللي هو ال lipoprotein
+
+71
+00:05:26,990 --> 00:05:30,710
+متصل معه lipid و protein و phospholipid، بيكون 
+
+72
+00:05:30,710 --> 00:05:35,630
+soluble in water، لأنه سطحه عبارة عن مجموعات polar
+
+73
+00:05:38,830 --> 00:05:42,970
+أنواع الـ lipoprotein الموجودة، في أن أنواع تقسمت
+
+74
+00:05:42,970 --> 00:05:47,310
+حسب الكثافة، حسب المكونات، و حسب الوظيفة، حسب ال
+
+75
+00:05:47,310 --> 00:05:52,490
+density، composition، function، كثافة و مكونات و وظيفة
+
+76
+00:05:52,490 --> 00:05:56,790
+من هذه أنواع الـ .. أنواع الـ lipoprotein في منه
+
+77
+00:05:56,790 --> 00:06:00,790
+كايلو مايكرون، و في أن الـ very low density
+
+78
+00:06:00,790 --> 00:06:05,250
+lipoprotein اللي هو الـ VLDL، و في أن الـ low density
+
+79
+00:06:05,250 --> 00:06:12,070
+lipoprotein اللي هو الـ LDL، و في عندنا الـ HDL اللي هو
+
+80
+00:06:12,070 --> 00:06:17,470
+الـ high density lipoprotein، إذا في عندنا إيش أنواع 
+
+81
+00:06:17,470 --> 00:06:24,810
+الـ lipoprotein، في منها كايلو مايكرون، VLDL، و HDL، ال
+
+82
+00:06:24,810 --> 00:06:29,390
+kylo micron هو مسئول عن الـ external lipid، عن اللبد 
+
+83
+00:06:29,390 --> 00:06:33,750
+اللي جاي للجسم من برا الجسم، مش اللي بيصنع الجسم، ال
+
+84
+00:06:33,750 --> 00:06:38,410
+kylo micron مسئول عن الدهون اللي بتيجي من الخارج
+
+85
+00:06:38,410 --> 00:06:43,570
+من الواجبات، من الواجبات اليومية، الكايلو مايكرون
+
+86
+00:06:43,570 --> 00:06:48,450
+مسئول عن الـ external lipid، الدايتري لبيد، توسع اللي
+
+87
+00:06:48,450 --> 00:06:51,930
+هو الدهون اللي جاية من الخارج، من خارج الجسم، من
+
+88
+00:06:51,930 --> 00:06:55,810
+الواجبات الجسم تناولها، مين المسئول عنها أنه يقوم 
+
+89
+00:06:55,810 --> 00:07:00,650
+بنقلها، هو عبارة عن الـ  كايلو مايكرون، الـ Very Low
+
+90
+00:07:00,650 --> 00:07:03,870
+Density Lipoprotein اللي هو الـ VLDL، و الـ Low
+
+91
+00:07:03,870 --> 00:07:07,030
+Density Lipoprotein الـ LDL، و High Density
+
+92
+00:07:07,030 --> 00:07:10,310
+Lipoprotein اللي هو الـ HDL، هم مسئولين عن الـ
+
+93
+00:07:10,310 --> 00:07:14,970
+Internal Lipid، عن اللبد الداخلي اللي بيصنع في
+
+94
+00:07:14,970 --> 00:07:20,620
+الـ liver، وظائفهم هدول، هذا المخطط بشكل عام، أنا 
+
+95
+00:07:20,620 --> 00:07:25,780
+أُلَخِّصُهُ إنه عندنا الـ Calomicron هي عبارة عن متخصصة
+
+96
+00:07:25,780 --> 00:07:29,600
+عن الـ External Libid اللي جاي من الدايتري، بتيجي
+
+97
+00:07:29,600 --> 00:07:33,300
+دهون اللي الإنسان بيتناول واجب الدهون، بتيجي هذه
+
+98
+00:07:33,300 --> 00:07:36,480
+الدهون بتصل للمعدة و الأمعاء، مين بدي ياخدها و
+
+99
+00:07:36,480 --> 00:07:40,060
+يعملها امتصاص و ينقلها؟ هو عبارة عن الـ Calomicron
+
+100
+00:07:40,060 --> 00:07:43,940
+مسئول عن الـ External Libid اللي جاية من الواجبات،
+
+101
+00:07:43,940 --> 00:07:48,280
+بياخدها، بينقلها، بيوزعها على خلايا الجسم، أما ال
+
+102
+00:07:48,280 --> 00:07:50,900
+internal lipid اللي فيه الـ liver اللي بيصنعه ال
+
+103
+00:07:50,900 --> 00:07:56,440
+liver مسئول عنها الـ LDL و VLDL و HDL، كل واحدة
+
+104
+00:07:56,440 --> 00:08:00,800
+وظيفتها، الـ HDL الـ high density lipoprotein كيف 
+
+105
+00:08:00,800 --> 00:08:06,960
+وظيفته؟ بيعمل على نقل الدهون من الخلايا، الذائد 
+
+106
+00:08:06,960 --> 00:08:10,100
+الدهون اللي بيكون ذائد في الجسم، هلجيب وزعنا دهون
+
+107
+00:08:10,100 --> 00:08:14,330
+على خلايا الجسم، اللي زاد في اتبعثر و في الـ blood
+
+108
+00:08:14,330 --> 00:08:20,190
+دهون زاد بيجي الـ HDL بيجمع هدول الدهون و بيرجعها
+
+109
+00:08:20,190 --> 00:08:25,510
+تاني على الـ liver، لذن الـ HDL carry cholesterol to
+
+110
+00:08:25,510 --> 00:08:31,310
+cells and deposit excess cholesterol in artery، لأ
+
+111
+00:08:31,310 --> 00:08:36,170
+الـ HDL بيعمل
+
+112
+00:08:36,170 --> 00:08:41,170
+على نقل الكوليسترول من خلايا الجسم، برجع تاني على
+
+113
+00:08:41,170 --> 00:08:47,670
+الـ liver، الـ LDL و الـ VLDL تقوم بالنقل، الليبد من 
+
+114
+00:08:47,670 --> 00:08:52,970
+الـ liver إلى خلايا الجسم، توازن، الـ كايلو مايكرو 
+
+115
+00:08:52,970 --> 00:08:56,270
+مسئول عن الدهون الخارجية بيوزعها، بتيجي الواجبة
+
+116
+00:08:56,270 --> 00:09:01,190
+و بيوزعها و بينقلها إلى خلايا الجسم، HDL مسئول على
+
+117
+00:09:01,190 --> 00:09:06,250
+أنه ينقل من خلايا الجسم يرجع تاني على الـ liver، LDL
+
+118
+00:09:06,250 --> 00:09:12,230
+و VLDL مسئول على أنه يوزع الكوليسترول من الـ liver 
+
+119
+00:09:12,230 --> 00:09:15,250
+من الكبد يوزع على خلايا الجسم
+
+120
+00:09:18,310 --> 00:09:22,610
+بحكي ليه أن الـ HDL اللي هو الـ high density of
+
+121
+00:09:22,610 --> 00:09:26,730
+the lipoprotein يترك الجلد يتلقى المزيد من 
+
+122
+00:09:26,730 --> 00:09:31,090
+الكوليسترول على أسطوله و يتركه إلى الجلد و يتعيد
+
+123
+00:09:31,090 --> 00:09:35,530
+إلى الجلد، الـ HDL زي ما حكينا بيغادر من الجلد، هو 
+
+124
+00:09:35,530 --> 00:09:39,130
+بيتصنع في الجلد، بيروح و بيطلع برا الجلد و بيروح
+
+125
+00:09:39,130 --> 00:09:42,670
+على خلايا الجسم، بيجمع الـ excess cholesterol
+
+126
+00:09:42,670 --> 00:09:46,290
+الكوليسترول الزيادة اللي ضايل في الجسم، بيرجعه تاني
+
+127
+00:09:46,290 --> 00:09:49,170
+على الـ liver، لقيت لما نجيب هدع الـ liver إيش بده 
+
+128
+00:09:49,170 --> 00:09:52,170
+يسوي فيه الـ liver؟ الـ liver excrete this
+
+129
+00:09:52,170 --> 00:09:55,650
+cholesterol into the bile then out of the body or
+
+130
+00:09:55,650 --> 00:10:00,250
+used in the bile salt production، لقيت الـ HDL جاب
+
+131
+00:10:00,250 --> 00:10:03,790
+للـ liver، جاب من خلايا الجسم كوليسترول، إيش بده يعمل 
+
+132
+00:10:03,790 --> 00:10:07,910
+فيه الـ liver؟ بحكي للـ liver إما بيعمل على إخراجه
+
+133
+00:10:07,910 --> 00:10:12,380
+إلى خارج الجسم مع الفضلات، إخراجه لخارج الجسم أو 
+
+134
+00:10:12,380 --> 00:10:16,240
+يستخدموا في تصنيع الـ bile salt، لأن الـ bile salt 
+
+135
+00:10:16,240 --> 00:10:20,480
+تصنع من الكوليسترول، فهو بيستخدموا في تصنيع هذا ال
+
+136
+00:10:20,480 --> 00:10:23,620
+liver، الكوليسترول اللي رجع على الـ liver إله طريقين
+
+137
+00:10:23,620 --> 00:10:29,080
+إما إخراجه خارج الجسم أو تصنيع الـ bile salt منه 
+
+138
+00:10:29,080 --> 00:10:33,240
+فالـ HDL لأنه بيعمل على remove excess كوليسترول 
+
+139
+00:10:33,240 --> 00:10:38,950
+from body، بنسميه protective أو good كوليسترول، لذن
+
+140
+00:10:38,950 --> 00:10:44,050
+الـ HDL بيجمع الكوليسترول من خلايا الجسم، بيرجعها
+
+141
+00:10:44,050 --> 00:10:47,630
+تاني على الـ liver، و الـ liver بيتخلص منها بطريقتين
+
+142
+00:10:47,630 --> 00:10:53,790
+إما بيتخلص منها بإخراجها خارج الجسم أو بعمل تصنيع
+
+143
+00:10:53,790 --> 00:10:58,490
+منها الـ bile salt، فلأن الـ HDL جمّع لي الدهون 
+
+144
+00:10:58,490 --> 00:11:02,530
+الزيادة في الجسم و اتخلص منها، بسميه protective
+
+145
+00:11:02,530 --> 00:11:06,290
+وقائي أو good cholesterol، اللي هو الكوليسترول
+
+146
+00:11:06,290 --> 00:11:10,390
+الجيد، لأنه خلصني من كمية الدهون الزيادة اللي في 
+
+147
+00:11:10,390 --> 00:11:14,510
+الجسم، لأن احنا زي ما احنا عارفين لو اتراكمت الدهون 
+
+148
+00:11:14,510 --> 00:11:18,480
+في الجسم، ممكن تعمل انسداد في الأوعية الدموية و في 
+
+149
+00:11:18,480 --> 00:11:22,820
+الشرايين، و تسبب جلطات، ف أنا إذا اتخلصت منها بيكون 
+
+150
+00:11:22,820 --> 00:11:27,780
+أفضل، ف الـ HDL بيخلصني منها، ف بسميه protective أو
+
+151
+00:11:27,780 --> 00:11:33,400
+good كوليسترول، الـ LDL بيعمل على transport 
+
+152
+00:11:33,400 --> 00:11:35,880
+كوليسترول throughout the body، delivering 
+
+153
+00:11:35,880 --> 00:11:38,820
+cholesterol to different tissues and organs، بيعمل 
+
+154
+00:11:38,820 --> 00:11:43,540
+على نقل الكوليسترول من الـ liver، بيوزع على الأعضاء
+
+155
+00:11:43,540 --> 00:11:47,460
+و الأنسجة، على الـ tissues و الـ organs، بيوزع من 
+
+156
+00:11:47,460 --> 00:11:50,760
+الكبد لخلايا الجسم، when the cell have all
+
+157
+00:11:50,760 --> 00:11:53,240
+cholesterol they need they reduce the number of
+
+158
+00:11:53,240 --> 00:11:55,680
+receptors or gates way of cholesterol to enter
+
+159
+00:11:55,680 --> 00:12:00,160
+الجسم، stuck، ف خلاص صارت وصلته كمية كفاية من ال
+
+160
+00:12:00,160 --> 00:12:06,400
+lipid، يفترض أنه يحفظ receptor، مستقبل أو قنوات، أنه
+
+161
+00:12:06,400 --> 00:12:10,720
+خلاص الـ LDL، إننا نعطيه إشارة إنه يوقف، إنه يبطل يجيب
+
+162
+00:12:10,720 --> 00:12:15,280
+كوليسترول للجسم، لأن الجسم أخذ حاجته، يفترض إنه فيه 
+
+163
+00:12:15,280 --> 00:12:20,020
+neuroreceptor شغال، مستقبل، يرسل إشارة إنه خلاص ما توصلش 
+
+164
+00:12:20,020 --> 00:12:24,440
+كوليسترول تاني للجسم يا LDL، وقف ما تشتغلش، الجسم 
+
+165
+00:12:24,440 --> 00:12:27,960
+عنده كوليسترول بكفاية، و ما تجيبلهوش تاني، as a
+
+166
+00:12:27,960 --> 00:12:31,020
+result، cholesterol contained in the LDL particles 
+
+167
+00:12:31,020 --> 00:12:35,230
+start to build up in the bloodstream، إذا هذا الـ
+
+168
+00:12:35,230 --> 00:12:39,550
+receptor ما اشتغلش، و كان فيه مشكلة مثلاً، الـ LDL هيظل
+
+169
+00:12:39,550 --> 00:12:45,150
+مستمر و يعمل على نقل الـ LDL، هيظل مستمر و ينقل
+
+170
+00:12:45,150 --> 00:12:49,530
+الكوليسترول من الـ liver لخلايا الجسم، يظل ينقل،
+
+171
+00:12:49,530 --> 00:12:53,670
+ينقل، طب الجسم استكفى، بدوش كوليسترول، هيظل ينقل،
+
+172
+00:12:53,670 --> 
+
+201
+00:15:04,790 --> 00:15:11,350
+والقلب لو البلاك هذه جلطات أجت فيه ووقفت مشجرة
+
+202
+00:15:11,350 --> 00:15:17,350
+القلب إذا أجت فيه الشريان المغذي للقلب بيصير عندي
+
+203
+00:15:17,350 --> 00:15:24,560
+heart attack اللي هو الذبحة القلبية إذا وقفت المجرة
+
+204
+00:15:24,560 --> 00:15:28,740
+أو الشريان المغذي للـ brain للدماغ بيصير عندي
+
+205
+00:15:28,740 --> 00:15:33,560
+stroke سكتة دماغية إذا تراكمت الدهون في
+
+206
+00:15:33,560 --> 00:15:37,880
+الشريان المغذي للقلب بيصير عندي heart attack وإذا
+
+207
+00:15:37,880 --> 00:15:41,960
+تراكمات في الشريان ووقفت الشريان المغذي للدماغ
+
+208
+00:15:41,960 --> 00:15:46,840
+بيصير عندي stroke سكتة دماغية عشان هيك الـ LDL
+
+209
+00:15:46,840 --> 00:15:52,160
+بنسميه bad cholesterol الكوليسترول السيء نطلق
+
+210
+00:15:52,160 --> 00:15:56,520
+على الـ LDL bad cholesterol لأنه بيعمل على نقل
+
+211
+00:15:56,520 --> 00:16:00,320
+الدهون من الـ liver إلى الجسم وممكن تتراكم هذه
+
+212
+00:16:00,320 --> 00:16:05,160
+الدهون في الجسم وتسبب جلطات تصلب الشرايين وممكن
+
+213
+00:16:05,160 --> 00:16:09,960
+تسبب سكتة دماغية و heart attack اللي هو الذبحة 
+
+214
+00:16:09,960 --> 00:16:14,970
+القلبية أو الصدرية معملنا هو عبارة عن isolation of
+
+215
+00:16:14,970 --> 00:16:18,010
+cholesterol from egg yolk حكينا مقدمة عن
+
+216
+00:16:18,010 --> 00:16:21,530
+الكوليسترول وعن أنه عقل لكن هسا بدنا ندخل في الجزء
+
+217
+00:16:21,530 --> 00:16:25,190
+العمل المعمل بيحكي أنه بدنا نعزل الكوليسترول من
+
+218
+00:16:25,190 --> 00:16:28,870
+صفار البيض الجد الـ egg كلها الـ egg اللي هي الـ
+
+219
+00:16:28,870 --> 00:16:32,370
+egg كاملة بتتكون من egg white بياض البيض ومن الـ
+
+220
+00:16:32,370 --> 00:16:37,030
+egg yolk اللي هو صفار البيض البيض بيحتوي على
+
+221
+00:16:37,030 --> 00:16:40,790
+مكوناته شو عبارة عن إيش؟ عبارة عن قلبه من أولبان
+
+222
+00:16:41,280 --> 00:16:45,900
+البيض عبارة عن قلبه من و لبن أما الصفار الصفار
+
+223
+00:16:45,900 --> 00:16:49,420
+البيض هو عبارة عن glycoprotein
+
+224
+00:16:54,080 --> 00:16:58,920
+Lipid حتى هو بيكون sorry  نايت تاني الـ hen egg الإيج
+
+225
+00:16:58,920 --> 00:17:02,460
+بيضة كاملة عبارة عن بياض البيض والصفار الـ egg
+
+226
+00:17:02,460 --> 00:17:05,800
+white و الـ egg yolk البياض الـ egg white هو عبارة
+
+227
+00:17:05,800 --> 00:17:10,040
+عن ألبومين هو عبارة عن بروتين فقط البياض عبارة عن
+
+228
+00:17:10,040 --> 00:17:13,660
+بروتين الألبومين أما الـ egg yolk اللي هو صفار البيض
+
+229
+00:17:13,660 --> 00:17:17,400
+هو عبارة عن lipid و glycoprotein عبارة عن بروتينات
+
+230
+00:17:17,400 --> 00:17:22,510
+سكرية مع دهون نجي للـ egg yolk اللي هو صفار البيض إيش
+
+231
+00:17:22,510 --> 00:17:28,450
+مكوناته؟ حكينا جلايكو بروتين ولبن عبارة عن 5% من
+
+232
+00:17:28,450 --> 00:17:34,750
+مكوناته هو كوليسترول و 66% جليسرايد و 28% فوسفوليبيد
+
+233
+00:17:34,750 --> 00:17:39,730
+هي توزيع الدهون الموجودة في صفار البيض منها 5%
+
+234
+00:17:39,730 --> 00:17:42,710
+كوليسترول اللي أنا بدي أخده أعزله بدي أسيب باقي
+
+235
+00:17:42,710 --> 00:17:48,610
+المكونات بس بدي كوليسترول الـ egg yolk بيحكي ليه
+
+236
+00:17:48,610 --> 00:17:52,030
+أنه غنية في الدهون صفار البيض هو غني بالدهون
+
+237
+00:17:52,030 --> 00:17:55,170
+بيحتوي على كمية عالية من الدهون من أشهر الدهون
+
+238
+00:17:55,170 --> 00:17:59,450
+اللي موجودة فيه هي الـ glyceride كوليسترول و
+
+239
+00:17:59,450 --> 00:18:04,410
+phosphatidylcholine أو اسمه الثاني ليسيتين إذا
+
+240
+00:18:04,410 --> 00:18:07,690
+صفار البيض يحتوي على الليبيد غني بالليبد أشهر
+
+241
+00:18:07,690 --> 00:18:12,920
+الليبيد اللي موجودة فيه الـ glyceride و الكوليسترول 
+
+242
+00:18:12,920 --> 00:18:16,320
+فوسفاتيديل كولين اللي هو ليسيتين احنا نعزل
+
+243
+00:18:16,320 --> 00:18:20,220
+الكوليسترول الكوليسترول اللي حكينا عنه اللي جينا نحكي
+
+244
+00:18:20,220 --> 00:18:23,120
+عن فوسفاتيديل كولين اشي بسيط عن فوسفاتيديل كولين
+
+245
+00:18:23,120 --> 00:18:27,840
+أو ليسيتين نفس الاسم فوسفاتيديل كولين أو ليسيتين هو
+
+246
+00:18:27,840 --> 00:18:32,240
+أشهر phospholipid موجود في الحيوان والنبات
+
+247
+00:18:32,240 --> 00:18:38,080
+فوسفاتيديل كولين أو ليسيتين هو أشهر phospholipid
+
+248
+00:18:38,080 --> 00:18:45,000
+موجود عند الحيوان والنبات وظائف هو له ثلاث وظائف
+
+249
+00:18:45,000 --> 00:18:48,580
+هو الـ key building block of membrane bilayer هو
+
+250
+00:18:48,580 --> 00:18:52,760
+التركيبة الأساسية للطبقة الثنائية في الـ membrane
+
+251
+00:18:52,760 --> 00:18:57,980
+يدخل في التركيبة الأساسية للـ membrane bilayer هي أول
+
+252
+00:18:57,980 --> 00:19:03,600
+وظيفة وظيفة ثانية هو بشكل نسبة عالية في الـ plasma
+
+253
+00:19:03,600 --> 00:19:08,860
+membrane الغلاف لـ plasma very high proportion
+
+254
+00:19:08,860 --> 00:19:12,900
+of the outer leaflet of the plasma membrane إذا هو
+
+255
+00:19:12,900 --> 00:19:16,300
+في الـ membrane bilayer وفيه الـ plasma membrane
+
+256
+00:19:16,300 --> 00:19:21,020
+ثالث وظيفة أنه هو عبارة عن الـ phospholipid الموجود
+
+257
+00:19:21,020 --> 00:19:25,450
+في الـ lipoprotein إذا فاكرين الـ lipoprotein حكينا
+
+258
+00:19:25,450 --> 00:19:30,530
+أنه هي عبارة عن lipid مع protein مع phospholipid
+
+259
+00:19:30,530 --> 00:19:35,310
+الـ phospholipid هاي هي عبارة عن فوسفاتيديل كولين أو
+
+260
+00:19:35,310 --> 00:19:39,750
+ليسيتين إذا هو ثلاث وظائف له وظيفة الأولى بيدخل في
+
+261
+00:19:39,750 --> 00:19:43,250
+تركيب الـ membrane bilayer وظيفة الثانية بيدخل في
+
+262
+00:19:43,250 --> 00:19:46,830
+تركيب البلازما membrane وظيفة الثالثة هو الـ
+
+263
+00:19:46,830 --> 00:19:52,830
+phospholipid الموجود في الـ lipoprotein أكثر فوسفو
+
+264
+00:19:52,830 --> 00:19:56,410
+أكثر ليبو بروتين يحتوي على فوسفاتيديل كولين هو
+
+265
+00:19:56,410 --> 00:20:01,510
+الـ HDL الليبو بروتين الحكينا فيه من HDL و LDL و
+
+266
+00:20:01,510 --> 00:20:06,530
+VLDL و الكايلو مايكون تركيبتهم تحتوي على فوسفو
+
+267
+00:20:06,530 --> 00:20:10,290
+ليبيد الفوسفو ليبيد هذا عبارة عن فوسفاتيديل كولين مين
+
+268
+00:20:10,290 --> 00:20:13,990
+أكثر واحد يحتوي على فوسفاتيديل كولين في تركيبه هو
+
+269
+00:20:13,990 --> 00:20:15,270
+الـ HDL
+
+270
+00:20:17,640 --> 00:20:21,180
+التجربة إن أنا بدي استخدم صفار البيض hard boiled
+
+271
+00:20:21,180 --> 00:20:25,060
+hen egg بدي أسلق بيضة بدي بيضة مسلوقة وبدي أخد
+
+272
+00:20:25,060 --> 00:20:30,000
+منها الصفار وبدي أستخدم أسيتون water bath flask
+
+273
+00:20:30,000 --> 00:20:38,540
+funnel filter paper و بيكر ديش نشوف كده أنا بدي
+
+274
+00:20:38,540 --> 00:20:41,260
+أعزل الكوليسترول من صفار البيض وأحسب الـ
+
+275
+00:20:41,260 --> 00:20:47,100
+percentage الـ
+
+276
+00:20:47,100 --> 00:20:50,100
+principle بناء على إيش أنا بدي أعزل الكوليسترول و
+
+277
+00:20:50,100 --> 00:20:54,720
+أسيب باقي المكونات بيحكي لي بناء على الـ solubility
+
+278
+00:20:54,720 --> 00:20:58,680
+in different organic solvents لجينا هم طب كل
+
+279
+00:20:58,680 --> 00:21:02,920
+المكونات لبن وكلهم هيدوبوا في نفس الأشياء كيف بدي
+
+280
+00:21:02,920 --> 00:21:05,860
+أخد الكوليسترول وأسيب كل الباقي اللبن بناء على الـ
+
+281
+00:21:05,860 --> 00:21:09,760
+solubility؟ بيحكي لي أنه وجدوا أن الكوليسترول هو
+
+282
+00:21:09,760 --> 00:21:13,780
+الوحيد اللي بيكون soluble in أسيتون لما نستخدم
+
+283
+00:21:13,780 --> 00:21:19,450
+أسيتون أحطه على صفار البيض بس الكوليسترول بيدوب
+
+284
+00:21:19,450 --> 00:21:24,190
+والباقي كله بيترسب كل المكونات بتترسب ما عدا
+
+285
+00:21:24,190 --> 00:21:29,330
+الكوليسترول بيدوب فالمبدأ أني أستخدم أسيتون يعمل
+
+286
+00:21:29,330 --> 00:21:34,610
+على إذابة الكوليسترول فقط وباقي المكونات بتكون
+
+287
+00:21:34,610 --> 00:21:40,790
+insoluble بتكون مترسبة إيش هو الأسيتون؟ الأسيتون
+
+288
+00:21:40,790 --> 00:21:45,470
+اسمه الآخر بروبانون بروبانون اسمه الآخر بروبانون
+
+289
+00:21:45,470 --> 00:21:50,650
+هو عبارة عن organic compound مركب عضوي له صيغة
+
+290
+00:21:50,650 --> 00:21:56,030
+CH3 لكل اثنين CO هو خصائصه colorless لا لون له
+
+291
+00:21:56,030 --> 00:22:02,490
+volatile متطاير flammable قابل للاشتعال وهو أبسط
+
+292
+00:22:02,490 --> 00:22:07,440
+كيتون هو كيتون يعني إيش صيغة الكيتون؟ أنه C double
+
+293
+00:22:07,440 --> 00:22:11,660
+bond O و R من جهة و R من جهة هو أبسط كيتون لأن
+
+294
+00:22:11,660 --> 00:22:14,600
+الـ R من جهة هي عبارة عن CH3 و الـ R من الجهة
+
+295
+00:22:14,600 --> 00:22:19,720
+الثانية هي CH3 برضه تمام، إذا الـ principle بناء
+
+296
+00:22:19,720 --> 00:22:23,460
+على استخدام الأسيتون اللي بيدوب بيدوب
+
+297
+00:22:23,460 --> 00:22:30,280
+الكوليسترول وباقي المكونات بتترسب إيش الخطوات؟ بدي
+
+298
+00:22:30,280 --> 00:22:34,890
+أجيب بيضة مسلوقة بدي أشيل عنها القشرة أجيب بيضة
+
+299
+00:22:34,890 --> 00:22:37,830
+مسلوقة أنا ما بديش القشرة بدي أتخلص منها وبدي أخد
+
+300
+00:22:37,830 --> 00:22:42,970
+الصفار بدي أخد نص الصفار نص الـ egg نص الصفار الـ
+
+301
+00:22:42,970 --> 00:22:48,330
+egg و أوزنها بدي أخد نص الصفار أوزنها بعدين بدي
+
+302
+00:22:48,330 --> 00:22:52,150
+أخد أحطها في بيكر وأطحنها أعمل mashed egg mashed
+
+303
+00:22:52,150 --> 00:22:57,130
+يعني أطحنها أحط عليها الـ acetone خمسة وعشرين مل
+
+304
+00:22:57,130 --> 00:23:01,610
+من الـ acetone وبعمل mix لمدة خمس دقائق إذا أنا
+
+305
+00:23:01,610 --> 00:23:05,810
+أخدت نص الصفار البيضة وزنته عشان هتهمني هذه
+
+306
+00:23:05,810 --> 00:23:11,110
+الوزن في الـ percentage في الحسابات بعدين بحطها في
+
+307
+00:23:11,110 --> 00:23:14,510
+بيكر هذا نص الصفر البيضة بحطها في بيكر وأطحنها
+
+308
+00:23:14,510 --> 00:23:19,320
+أحط عليها خمسة وعشرين مل من الأسيتون وبعدين اضني 
+
+309
+00:23:19,320 --> 00:23:24,060
+أحرك لمدة خمس دقائق بعد خمس دقائق يفترض الـ acetone
+
+310
+00:23:24,060 --> 00:23:29,320
+يخليها بيترسب كل المكونات في الراسب وفوق السائل
+
+311
+00:23:29,320 --> 00:23:33,660
+يحتوي على acetone مع كوليسترول أنا بدي السائل ما بديش
+
+312
+00:23:33,660 --> 00:23:37,480
+الراسب اللي تحت بدي السائل اللي فوق اللي هو عبارة
+
+313
+00:23:37,480 --> 00:23:41,440
+عن acetone وكوليسترول لأن الكوليسترول الوحيد اللي بيدوب
+
+314
+00:23:41,440 --> 00:23:45,060
+في الـ Acetone إيش بدي أعمل؟ بدي أسحب أحاول
+
+315
+00:23:45,060 --> 00:23:48,980
+أسحب السائل هذا اللي فوق أسحب الطبقة اللي فوق بـ
+
+316
+00:23:48,980 --> 00:23:52,740
+dropper أو بأي شيء بدي أسحب الطبقة اللي فوق السائل
+
+317
+00:23:52,740 --> 00:23:57,660
+وأحطه في بيكر ثاني وأسكر عليها عشان ما يطيرش
+
+318
+00:23:57,660 --> 00:24:02,940
+الأسيتون أرجع ثاني على الراسب اللي ظل عندي في
+
+319
+00:24:02,940 --> 00:24:06,920
+البيكر الـ recipe اللي تحت بأضيف عليها كمان 25 مل
+
+320
+00:24:06,920 --> 00:24:11,530
+من الأسيتون وأعمل mix أكرر الخطوة اللي قبلها أنا
+
+321
+00:24:11,530 --> 00:24:16,310
+جبت نص صفر البيض وطحنته وحطيت عليه 25 مل من
+
+322
+00:24:16,310 --> 00:24:20,650
+الأسيتون وعملت mix هيكون فوق في السائل في عندي
+
+323
+00:24:20,650 --> 00:24:24,470
+أسيتون مع كوليسترول وعندي راسب تحت بدي أخد السائل
+
+324
+00:24:24,470 --> 00:24:28,340
+هذا اللي فوق أنقله على بيكر ثاني وبعدين الطبقة
+
+325
+00:24:28,340 --> 00:24:31,520
+اللي تحتها دي عشان أتأكد إن أخد كل الكوليسترول
+
+326
+00:24:31,520 --> 00:24:37,500
+بضيف كمان 25 مل أسيتون وأضلني أحرك وبعدين أخد
+
+327
+00:24:37,500 --> 00:24:39,760
+السائل اللي فوق اللي هو عبارة عن أسيتون و
+
+328
+00:24:39,760 --> 00:24:43,440
+كوليسترول أضيفه على الجزء الأول على الـ beaker اللي
+
+329
+00:24:43,440 --> 00:24:48,360
+نقلته من الجزء الأول بيصير عندي هيك 50 مل 25 مرة
+
+330
+00:24:48,360 --> 00:24:51,700
+الأولى و 25 مرة الثانية عبارة عن أسيتون مع
+
+331
+00:24:51,700 --> 00:24:57,530
+كوليسترول هالجيت بديش أنا مين؟ عشان أتأكد إن أنا
+
+332
+00:24:57,530 --> 00:25:02,410
+ما أخدتش راسب بده أعمل فلترة بعد ما نقلت الأسيتون و
+
+333
+00:25:02,410 --> 00:25:05,670
+الكوليسترول بده أعمل فلترة بده أجيب فلاسك وأجيب
+
+334
+00:25:05,670 --> 00:25:11,100
+فيها funnel محّجن و filter paper وأضيره في هذا الـ
+
+335
+00:25:11,100 --> 00:25:14,260
+filter paper على الـ filter paper خليه يـ filter
+
+336
+00:25:14,260 --> 00:25:18,840
+هينزل عندي السائل ومين هو السائل؟ السائل عبارة عن
+
+337
+00:25:18,840 --> 00:25:22,140
+أسيتون مع كوليسترول أما الراسب اللي بيضل في الـ
+
+338
+00:25:22,140 --> 00:25:26,220
+filter paper هذا ما بديش إياه أنا بدي السائل اللي هو
+
+339
+00:25:26,220 --> 00:25:30,140
+يحتوي على كوليسترول بأخد السائل هذا من الـ flask
+
+340
+00:25:30,140 --> 00:25:33,760
+بعد ما يخلص فلترة بدي أرمي الورقة وأخد السائل
+
+341
+00:25:33,760 --> 00:25:37,940
+السائل هذا عبارة عن أسيتون مع كوليسترول ما بديش
+
+342
+00:25:37,940 --> 00:25:41,760
+الأسيتون بدي بس كوليسترول الأسيتون حكينا من خصائصه
+
+343
+00:25:41,760 --> 00:25:46,170
+أنه volatile أنه متطاير تمام؟ فإيش بدي أسوي؟ بدي أخده
+
+344
+00:25:46,170 --> 00:25:50,470
+في water bath في ماء ساخن بدي أحط الـ beaker المي
+
+345
+00:25:50,470 --> 00:25:54,770
+الساخنة بتساعد على أنه يطير الأسيتون بسرعة بيطير
+
+346
+00:25:54,770 --> 00:25:59,450
+الأسيتون وبيضل عندي بس كوليسترول بأخده وبحطه على
+
+347
+00:25:59,450 --> 00:26:03,630
+ثلج على ice عشان يعمل heat shock صدمة حرارية
+
+348
+00:26:03,630 --> 00:26:08,010
+للكوليسترول أنه يتجمع مع بعض بيتجمع عندي على شكل
+
+349
+00:26:08,010 --> 00:26:14,390
+crystal الكوليسترول بيتجمع على شكل crystal هذه
+
+350
+00:26:14,390 --> 00:26:18,910
+الـ crystal هي عبارة عن الكوليسترول بأخدها وبوزنها
+
+351
+00:26:18,910 --> 00:26:22,990
+يعني البيكر بكون وزنته من الأول وزن وزنته وعارف
+
+352
+00:26:22,990 --> 00:26:27,530
+جدا ش وزنته عشان أنقص بعد هيك الوزن لأنه لما أوزن
+
+353
+00:26:27,530 --> 00:26:30,690
+الكوليسترول في البيكر هيوزنني اثنين مع بعض
+
+354
+00:26:30,690 --> 00:26:35,730
+كوليسترول مع البيكر فأنقص الوزن زي ما عملنا أيام
+
+355
+00:26:35,730 --> 00:26:39,450
+الـ casein لما وزننا الورقة وبعدين وزننا الورقة مع
+
+356
+00:26:39,450 --> 00:26:44,360
+الكازين تمام وبأخد وزن الكوليسترول وبدي أحسبه
+
+357
+00:26:44,360 --> 00:26:48,200
+الـ percentage الـ percentage للكوليسترول إيش بتساوي
+
+358
+00:26:48,200 --> 00:26:51,440
+weight of الكوليسترول وزن الكوليسترول اللي طلعت
+
+359
+00:26:51,440 --> 00:26:55,480
+معاكي في الآخر اللي وزنّتيها على وزن نص البيضة اللي
+
+360
+00:26:55,480 --> 00:27:00,180
+وزنّيناها في الأول لما حكينا أنه نص صفار اللي أخذناه
+
+361
+00:27:00,180 --> 00:27:06,000
+ضرب 100% وبأحسب وزن الكوليسترول اللي موجودة في
+
+362
+00:27:06,000 --> 00:27:14,050
+صفار البيض وهيك بيكون خلصنا هذا المعمل ويعطيكم
+
+363
+00:27:14,050 --> 00:27:14,490
+العافية 
diff --git a/PL9fwy3NUQKwZ1Q7zOg1ObgufPMjRI3WY1/9CmoB4eQs2I_postprocess.srt b/PL9fwy3NUQKwZ1Q7zOg1ObgufPMjRI3WY1/9CmoB4eQs2I_postprocess.srt
new file mode 100644
index 0000000000000000000000000000000000000000..850cda3b1dfe77ebbd2125ed0cfd0e2dded58261
--- /dev/null
+++ b/PL9fwy3NUQKwZ1Q7zOg1ObgufPMjRI3WY1/9CmoB4eQs2I_postprocess.srt
@@ -0,0 +1,1452 @@
+1
+00:00:00,000 --> 00:00:05,280
+كيفي موضوع جديد هو isolation of cholesterol from
+
+2
+00:00:05,280 --> 00:00:11,100
+egg yolk عزل الكوليسترول من صفار البيضة، نجيب صفار
+
+3
+00:00:11,100 --> 00:00:16,450
+بيضة و نعزل منها الكوليسترولبحكي اليوم الحاجة ان
+
+4
+00:00:16,450 --> 00:00:19,970
+اتعرف على الكلسترول الكلسترول هو most abundant
+
+5
+00:00:19,970 --> 00:00:25,290
+steroid in the body هو أشهر الكلسترول هو أشهر
+
+6
+00:00:25,290 --> 00:00:29,870
+steroid أشهر libid موجود في الجسم أشهر libid موجود
+
+7
+00:00:29,870 --> 00:00:35,110
+في الجسم هو الكلسترول is a waxy substance found in
+
+8
+00:00:35,110 --> 00:00:40,690
+all of the body cell هو عبارة عن مادة شمعية قوامه
+
+9
+00:00:40,690 --> 00:00:47,020
+شمعيموجود في كل أجزاء الجسم كيف تركبته؟ تركبته
+
+10
+00:00:47,020 --> 00:00:53,040
+عبارة عن Methyl group اللي هي CH3 مع Alkyl Chain
+
+11
+00:00:53,040 --> 00:00:59,240
+اللي هي سلسلة منطبط فيها CH3 و OH و Steroid
+
+12
+00:00:59,240 --> 00:01:02,860
+nucleus Steroid nucleus هو عبارة عن الأربع حلقات
+
+13
+00:01:02,860 --> 00:01:08,480
+اللي تحت متصل فيهم OH و سلسلة بنسميها Alkyl Chainو
+
+14
+00:01:08,480 --> 00:01:13,940
+CH3 مرتبطة في هذه السلسلة هذه عبارة عن تركيبة
+
+15
+00:01:13,940 --> 00:01:20,050
+الكوليسترول الـ Structure Aإيش وظائف الكلسترول ال
+
+16
+00:01:20,050 --> 00:01:23,310
+function؟ أول وظيفة help and maintain cell
+
+17
+00:01:23,310 --> 00:01:28,230
+membrane يعمل على الحفاظ و حماية ال cell membrane
+
+18
+00:01:28,230 --> 00:01:33,230
+بيحمي و يحافظ على ال cell membrane وظيفة تانية
+
+19
+00:01:33,230 --> 00:01:37,890
+protect and insulate nerve fiber بيعمل على حماية و
+
+20
+00:01:37,890 --> 00:01:43,910
+عزل الألياف العصبية حماية و عزل الألياف العصبية
+
+21
+00:01:45,300 --> 00:01:49,020
+تلاتة involved in the formation of sex hormones زي
+
+22
+00:01:49,020 --> 00:01:54,040
+الاستروجين يدخل في تركيب ال sex hormones الهرمونات
+
+23
+00:01:54,040 --> 00:01:58,620
+الجنسية زي الاستروجين بيدخل في تركيبها الكوليسترون
+
+24
+00:01:58,620 --> 00:02:02,800
+أربع production of bile salt which help digesting
+
+25
+00:02:02,800 --> 00:02:07,100
+fat زي ما حكينا المعمل اللي فات هو يدخل في تصنيع
+
+26
+00:02:07,100 --> 00:02:10,900
+ال bile salt اللي هي أملاح العصارة السفراوية التي
+
+27
+00:02:10,900 --> 00:02:17,840
+تساعد على هضم الدهونحكينا عنها المعمل اللي فات كيف
+
+28
+00:02:17,840 --> 00:02:21,240
+الكوليسترول موجود في الجسم؟ هلجيت الكوليسترول من
+
+29
+00:02:21,240 --> 00:02:25,960
+أين يحصل عليه الجسم؟ 80% من الكوليسترول بتصنع في
+
+30
+00:02:25,960 --> 00:02:31,460
+ال liver sensitized in the liver بالنسبة 80% و 20%
+
+31
+00:02:31,460 --> 00:02:36,220
+بناخدها من الواجبات موجودة في الحليب، في البيض، في
+
+32
+00:02:36,220 --> 00:02:43,280
+اللحمة، 20% أما 80% بيصنع الجسم في الكبتليش بيحتاج
+
+33
+00:02:43,280 --> 00:02:46,480
+الجسم للكوليسترول بناء على وضعفه بيحتاج لل cell
+
+34
+00:02:46,480 --> 00:02:49,480
+membrane لحمية ال cell membrane لعزل ال nerve
+
+35
+00:02:49,480 --> 00:02:53,720
+tissue العزل الألياف العصبية في تصنيع ال steroid
+
+36
+00:02:53,720 --> 00:02:57,960
+hormone في تصنيع vitamin D هدول كل استخدامات
+
+37
+00:02:57,960 --> 00:02:59,120
+للكوليسترول
+
+38
+00:03:01,080 --> 00:03:05,520
+الكلسترول النسبة الطبيعية له في الجسم أن يكون أقل
+
+39
+00:03:05,520 --> 00:03:10,240
+من 200 ميلي جرام per deciliter كلسترول النسبة
+
+40
+00:03:10,240 --> 00:03:14,600
+الطبيعية له في الجسم أن يكون أقل من 200 ميلي جرام
+
+41
+00:03:14,600 --> 00:03:20,400
+per deciliter إذا زاد elevated plasma cholesterol
+
+42
+00:03:20,400 --> 00:03:23,820
+exceeds 200 ميلي جرام per deciliter زاد عن 200
+
+43
+00:03:23,820 --> 00:03:26,940
+ميلي جرام per deciliter بنسمي هذه الحالة
+
+44
+00:03:26,940 --> 00:03:31,410
+كلسترليمياالـ Cholestrolimia معناها ارتفاع
+
+45
+00:03:31,410 --> 00:03:36,350
+الكوليسترول أكتر من 200 ميلي جرام بر دي سيلتان
+
+46
+00:03:36,350 --> 00:03:40,170
+وهذا إذا ارتفع بيصير اندي clots, arteries وانهي
+
+47
+00:03:40,170 --> 00:03:44,950
+level 4 مبلاتين بيصير انسداد في الشرايين والأوردة
+
+48
+00:03:44,950 --> 00:03:46,730
+وممكن تسبب جلطات
+
+49
+00:03:50,000 --> 00:03:53,240
+انا عبارة عن جدول بيوضح الأطعم اللي بتحتوي على
+
+50
+00:03:53,240 --> 00:03:57,280
+الكوليسترون مش مهمة كتير هي موجودة في الكبدة،
+
+51
+00:03:57,280 --> 00:04:00,800
+موجودة في البيض، موجودة في سرطان البحر، في الدجاج
+
+52
+00:04:00,800 --> 00:04:04,640
+المقلي، في الهامبورجر، في الحليب، في المارجارينة،
+
+53
+00:04:04,640 --> 00:04:07,600
+موجودة في أشياء كتير، موجودة كوليسترون، لكن نسبة
+
+54
+00:04:07,600 --> 00:04:14,340
+اللي بنحصل عليها من الجسم هي فقط 20% في عندنا حاجة
+
+55
+00:04:14,340 --> 00:04:19,130
+اسمها lipoproteinالـ lipoprotein إيش هي؟ هلجيت
+
+56
+00:04:19,130 --> 00:04:23,790
+اللبد هو non-polar بقدرش يدوب في الماء فكيف بده
+
+57
+00:04:23,790 --> 00:04:27,670
+يمشي في الجسم هذا اللبد؟ هلجيت اللبد لأنه non
+
+58
+00:04:27,670 --> 00:04:32,510
+-polar بيحتاج إلى نواقل تنقله في الجسم النواقل هذه
+
+59
+00:04:32,510 --> 00:04:37,730
+عبارة عن lipoprotein ال lipoprotein هي نواقل اللبد
+
+60
+00:04:37,730 --> 00:04:43,150
+هي نواقل لنقل اللبد لأنه اللبد بقدرش يتحرك لأنه
+
+61
+00:04:43,150 --> 00:04:48,580
+non-polarالليبوبروتين هي عبارة عن لبد مع بروتين و
+
+62
+00:04:48,580 --> 00:04:53,480
+عليها كمان فوسفوليبن الليبوبروتين هي عبارة عن لبد
+
+63
+00:04:53,480 --> 00:04:58,040
+مع بروتين و فوسفوليبن اللي جات اللبد لما يتصل على
+
+64
+00:04:58,040 --> 00:05:02,580
+سطحه بروتين و فوسفوليبن بتعطيه خاصية أنه يصير
+
+65
+00:05:02,580 --> 00:05:06,640
+soluble in water لأن ال surface بيكون عبارة عن
+
+66
+00:05:06,640 --> 00:05:10,560
+polar lipid بتكون الخارج السطح المحاط في هذا اللبد
+
+67
+00:05:10,560 --> 00:05:14,250
+هو عبارة عنمواد polar اللي هي ال phospholipid ال
+
+68
+00:05:14,250 --> 00:05:18,870
+phosphatic group بتكون على السطح فبيكون كأنه polar
+
+69
+00:05:18,870 --> 00:05:23,550
+بيكون soluble in water ليش؟ لإتصاله بمجموعة خلته
+
+70
+00:05:23,550 --> 00:05:26,990
+polar زي ال phospholipid اللي هو ال lipoprotein
+
+71
+00:05:26,990 --> 00:05:30,710
+متصل معه lipid و protein و phospholipid بيكون
+
+72
+00:05:30,710 --> 00:05:35,630
+soluble in water لأنه سطحه عبارة عن مجموعات polar
+
+73
+00:05:38,830 --> 00:05:42,970
+أنواع الـ lipoprotein الموجودة في أن أنواع تقسمت
+
+74
+00:05:42,970 --> 00:05:47,310
+حسب الكثافة حسب المكونات وحسب الوظيفة حسب ال
+
+75
+00:05:47,310 --> 00:05:52,490
+density composition function كثافة وكونات ووظيفة
+
+76
+00:05:52,490 --> 00:05:56,790
+من هذه أنواع ال .. أنواع ال lipoprotein في منه
+
+77
+00:05:56,790 --> 00:06:00,790
+كايلو مايكرون وفي أن ال very low density
+
+78
+00:06:00,790 --> 00:06:05,250
+lipoprotein اللي هو VLDL وفي أن ال low density
+
+79
+00:06:05,250 --> 00:06:12,070
+lipoprotein اللي هو LDLو في عندنا ال HDL اللي هو
+
+80
+00:06:12,070 --> 00:06:17,470
+ال high density lipoprotein اذا في عندنا ايش انواع
+
+81
+00:06:17,470 --> 00:06:24,810
+ال lipoprotein في منها كايلو مايكرون VLDL و HDLال
+
+82
+00:06:24,810 --> 00:06:29,390
+kylo micron هو مسئول عن ال external لبد عن اللبد
+
+83
+00:06:29,390 --> 00:06:33,750
+اللي جاي للجسم من برا الجسم مش اللي بيصنع الجسم ال
+
+84
+00:06:33,750 --> 00:06:38,410
+kylo micron مسئول عن الدهون اللي بتيجي من الخارج
+
+85
+00:06:38,410 --> 00:06:43,570
+من الواجبات من الواجبات اليوميةالكايلو مايكرون
+
+86
+00:06:43,570 --> 00:06:48,450
+مسئول عن الـexternal lipid الديوتري لبد توسع اللي
+
+87
+00:06:48,450 --> 00:06:51,930
+هو الدهون اللي جاية من الخارج من خارج الجسم من
+
+88
+00:06:51,930 --> 00:06:55,810
+الواجبات الجسم اتناولها مين المسئول عنها انه يقوم
+
+89
+00:06:55,810 --> 00:07:00,650
+بنقلها هو عبارة عن الكايلو مايكرونالـ Very Low
+
+90
+00:07:00,650 --> 00:07:03,870
+Density Lipoprotein اللي هو الـ VLDL والـ Low
+
+91
+00:07:03,870 --> 00:07:07,030
+Density Lipoprotein الـ LDL وHigh Density
+
+92
+00:07:07,030 --> 00:07:10,310
+Lipoprotein اللي هو الـ HDL هم مسئولين عن الـ
+
+93
+00:07:10,310 --> 00:07:14,970
+Internal Lipid عن اللبد الداخلي اللي بيصنع في
+
+94
+00:07:14,970 --> 00:07:20,620
+الليفانكيف وظائفهم هدول، هذا المخطط بشكل عام أنا
+
+95
+00:07:20,620 --> 00:07:25,780
+ألخصه إنه عندنا الـ Calomicron هي عبارة عن متخصصة
+
+96
+00:07:25,780 --> 00:07:29,600
+عن الـ External Libid اللي جاي من الدياتري، بتيجي
+
+97
+00:07:29,600 --> 00:07:33,300
+دهون اللي الانسان بيتناول واجب الدهون، بتيجي هذه
+
+98
+00:07:33,300 --> 00:07:36,480
+الدهون بتصل للمعدة و الأمعاء، مين بدي ياخدها و
+
+99
+00:07:36,480 --> 00:07:40,060
+يعملها امتصاص و ينقلها؟ هو عبارة عن ال Calomicron
+
+100
+00:07:40,060 --> 00:07:43,940
+مسئول عن ال External Libid اللي جاية من الواجبات،
+
+101
+00:07:43,940 --> 00:07:48,280
+بياخدها، بينقلها، بوزحها على خلايا الجسمأما ال
+
+102
+00:07:48,280 --> 00:07:50,900
+internal lipid اللي فيه ال liver اللي بيصنحها ال
+
+103
+00:07:50,900 --> 00:07:56,440
+liver مسئول عنها ال LDL و VLDL و HDL كل واحدة
+
+104
+00:07:56,440 --> 00:08:00,800
+وظيفته ال HDL ال high density lipoprotein كيف
+
+105
+00:08:00,800 --> 00:08:06,960
+موظيفته؟ بيعمل على نقل الدهون من الخلايا الذائد
+
+106
+00:08:06,960 --> 00:08:10,100
+الدهون اللي بيكون ذائد في الجسم هالجت وزعنا دهون
+
+107
+00:08:10,100 --> 00:08:14,330
+على خلايا الجسمالذي زاد في اتشع و في الـ blood
+
+108
+00:08:14,330 --> 00:08:20,190
+دهون زاد بيجي الـ HDL بيجمع هدول الدهون و بيرجحها
+
+109
+00:08:20,190 --> 00:08:25,510
+تاني على الـ liver لذن الـ HDL carry كلسترول to
+
+110
+00:08:25,510 --> 00:08:31,310
+cell and deposit excess cholesterol in arteryلأ
+
+111
+00:08:31,310 --> 00:08:36,170
+الـ HDL يعمل
+
+112
+00:08:36,170 --> 00:08:41,170
+على نقل الكوليسترول من خلايا الجسم برجع تاني على
+
+113
+00:08:41,170 --> 00:08:47,670
+الليفر الـ LDL و الـ VLDL تقوم بالنقل الليبد من
+
+114
+00:08:47,670 --> 00:08:52,970
+الليفر إلى خلايا الجسم التوازنةالقايلو مايكرو
+
+115
+00:08:52,970 --> 00:08:56,270
+مسئول عن الدهون الخارجية بيوزحها بتيجي الواجبة
+
+116
+00:08:56,270 --> 00:09:01,190
+وبيوزحها وبينقلها إلى خلايا الجسم HDL مسئول على
+
+117
+00:09:01,190 --> 00:09:06,250
+أنه ينقل من خلايا الجسم يرجع تاني على ال liver LDL
+
+118
+00:09:06,250 --> 00:09:12,230
+و VLDL مسئول على أنه يوزع الكوليسترول من ال liver
+
+119
+00:09:12,230 --> 00:09:15,250
+من الكبد يوزع على خلايا الجسم
+
+120
+00:09:18,310 --> 00:09:22,610
+بحكي ليه أن الـ HDL اللي هو الـ high density of
+
+121
+00:09:22,610 --> 00:09:26,730
+the lipoprotein يترك الجلد يتلقى المزيد من
+
+122
+00:09:26,730 --> 00:09:31,090
+الكوليسترول على أسطوله و يتركه إلى الجلد و يتعيد
+
+123
+00:09:31,090 --> 00:09:35,530
+إلى الجلد الـ HDL زي ما حكينا بيغادر من الجلد، هو
+
+124
+00:09:35,530 --> 00:09:39,130
+بيتصنع في الجلد، بيروح و بيطلع برا الجلد و بيروح
+
+125
+00:09:39,130 --> 00:09:42,670
+على خلايا الجسمبيجمع الـ excess cholesterol
+
+126
+00:09:42,670 --> 00:09:46,290
+الكوليسترول الزيادة اللي ضايل في الجسم بيرجعه تاني
+
+127
+00:09:46,290 --> 00:09:49,170
+على ال liver لقيت لما نجيب هدع ال liver إيش بده
+
+128
+00:09:49,170 --> 00:09:52,170
+يسوي فيه ال liver؟ ال liver excrete this
+
+129
+00:09:52,170 --> 00:09:55,650
+cholesterol into the bile then out of the body or
+
+130
+00:09:55,650 --> 00:10:00,250
+used in the bile salt production لقيت ال HDL جاب
+
+131
+00:10:00,250 --> 00:10:03,790
+لل liver جاب من خلايا الجسم كوليسترول إيش بده يعمل
+
+132
+00:10:03,790 --> 00:10:07,910
+فيه ال liver؟ بحكي لل liver إما بيعمل على إخراجه
+
+133
+00:10:07,910 --> 00:10:12,380
+إلى خارج الجسممع الفضلات إخراجه لخارج الجسم أو
+
+134
+00:10:12,380 --> 00:10:16,240
+يستخدموا في تصنيع ال bile salt لأن ال bile salt
+
+135
+00:10:16,240 --> 00:10:20,480
+تصنع من الكوليسترول فهو بيستخدموا في تصنيع هذا ال
+
+136
+00:10:20,480 --> 00:10:23,620
+liver الكوليسترول اللي رجع على ال liver إله طريقيا
+
+137
+00:10:23,620 --> 00:10:29,080
+إما إخراجه خارج الجسم أو تصنيع ال bile salt منه
+
+138
+00:10:29,080 --> 00:10:33,240
+فال HDL لأنه بيعمل على remove excess كوليسترول
+
+139
+00:10:33,240 --> 00:10:38,950
+from body بنسمي protective أو good كوليسترونلذن
+
+140
+00:10:38,950 --> 00:10:44,050
+الـ HDL بيجمع الكوليسترول من خلايا الجسم بيرجحها
+
+141
+00:10:44,050 --> 00:10:47,630
+تاني على ال liver وال liver بيتخلص منها بطريقتين
+
+142
+00:10:47,630 --> 00:10:53,790
+إما بيتخلص منها بإخراجها خارج الجسمأو بعمل تصنيع
+
+143
+00:10:53,790 --> 00:10:58,490
+منها الـ bile salt فلأن الـ HDL جمّع لي الدهون
+
+144
+00:10:58,490 --> 00:11:02,530
+الزيادة في الجسم واتخلص منها بسميه protective
+
+145
+00:11:02,530 --> 00:11:06,290
+وقائي أو good cholesterol اللي هو الكوليسترول
+
+146
+00:11:06,290 --> 00:11:10,390
+الجيد لأنه خلصني من كمية الدهون الزيادة اللي في
+
+147
+00:11:10,390 --> 00:11:14,510
+الجسم لأن احنا زي ما احنا عارفين لو اتراكمت الدهون
+
+148
+00:11:14,510 --> 00:11:18,480
+في الجسمممكن تعمل انسداد في الأوعى الدموية وفي
+
+149
+00:11:18,480 --> 00:11:22,820
+الشرايين وتسبب جلطات فانا إذا اتخلصت منها بيكون
+
+150
+00:11:22,820 --> 00:11:27,780
+أفضل ف الـHDL بيخلصني منها فبسميه protective أو
+
+151
+00:11:27,780 --> 00:11:33,400
+good كوليسترولالـ LDL بيعمل على transport
+
+152
+00:11:33,400 --> 00:11:35,880
+كوليسترول throughout the body delivering
+
+153
+00:11:35,880 --> 00:11:38,820
+cholesterol to different tissue and organs بيعمل
+
+154
+00:11:38,820 --> 00:11:43,540
+على نقل الكوليسترول من الـ liver بيوزع على الأعضاء
+
+155
+00:11:43,540 --> 00:11:47,460
+والأنسجة، على ال tissue و ال organs، بيوزع من
+
+156
+00:11:47,460 --> 00:11:50,760
+الكبد لخلايا الجسمwhen the cell have all
+
+157
+00:11:50,760 --> 00:11:53,240
+cholesterol they need they reduce the number of
+
+158
+00:11:53,240 --> 00:11:55,680
+receptors or gates way of cholesterol to enter
+
+159
+00:11:55,680 --> 00:12:00,160
+الجسم stuck فخلاص صارت وصلته كمية كفاية من ال
+
+160
+00:12:00,160 --> 00:12:06,400
+lipid يفترض انه يحفظ receptor مستقبل او قنوات انه
+
+161
+00:12:06,400 --> 00:12:10,720
+خلاص ال LDLإننا نعطيه إشارة إنه يوقف إنه يبطل يجيب
+
+162
+00:12:10,720 --> 00:12:15,280
+كوليسترول للجسم لأن الجسم أخد حاجته يفترض إنه فيه
+
+163
+00:12:15,280 --> 00:12:20,020
+نيروسيبتر شغال مستقبل يرسل إشارة إنه خلاص ما توصلش
+
+164
+00:12:20,020 --> 00:12:24,440
+كوليسترول تاني للجسم يا LDL وقف ما تشتغلش الجسم
+
+165
+00:12:24,440 --> 00:12:27,960
+عنده كوليسترول بكفايت وما تجيبلهوش تاني as a
+
+166
+00:12:27,960 --> 00:12:31,020
+result كوليسترول contained in the LDL particles
+
+167
+00:12:31,020 --> 00:12:35,230
+start to build up in the bloodstreamإذا هذا الـ
+
+168
+00:12:35,230 --> 00:12:39,550
+Receptor ماشتغلش وكان فيه مشكلة مثلا الـ LDL هيظل
+
+169
+00:12:39,550 --> 00:12:45,150
+مستمر ويعمل على نقل الـ LDL هيظل مستمر وينقل
+
+170
+00:12:45,150 --> 00:12:49,530
+الكوليسترول من الـ liver لخلايا الجسم يظل ينقل،
+
+171
+00:12:49,530 --> 00:12:53,670
+ينقل، طب الجسم استكفى، بدوش كوليسترول، هيظل ينقل،
+
+172
+00:12:53,670 --> 00:12:58,690
+ينقل، ينقل، لحد ما هذه الجزيئات لزيادة تبني نفسها
+
+173
+00:12:58,690 --> 00:13:03,210
+وتستقر في الـ blood stream، في الأوعية الدمويةمع
+
+174
+00:13:03,210 --> 00:13:06,310
+الوجد الكوليسترول هذا اللي بيكون في الأوعية
+
+175
+00:13:06,310 --> 00:13:10,410
+الدموية جاعت بيصير عليه oxidize بيصير عليه عمليات
+
+176
+00:13:10,410 --> 00:13:15,310
+أكسدة عمليات أكسدة يعني يتكسر بيتكسر إلى حجم أصغر
+
+177
+00:13:15,310 --> 00:13:20,110
+reduce inside هلجيت؟ الليبد صار متكون في الأوعية
+
+178
+00:13:20,110 --> 00:13:24,490
+الدموية وحجمه صغير تمام؟ هذه اللي حجمها صغير
+
+179
+00:13:24,490 --> 00:13:26,230
+الليبد اللي حجمها صغير
+
+180
+00:13:28,880 --> 00:13:32,240
+صارت متراكمة في الأوعية الدموية small particles
+
+181
+00:13:32,240 --> 00:13:36,420
+more easily enter the blood vessels فسهل عليها
+
+182
+00:13:36,420 --> 00:13:40,060
+أنها تدخل أي وعاء دموي، أي شريان، أي وريد، لأنها
+
+183
+00:13:40,060 --> 00:13:43,760
+صارت صغيرة، حجمها صغير وبتقدر تدخل على الشراين
+
+184
+00:13:43,760 --> 00:13:47,640
+والأوردة، بتبدأ تتراكم في الداخل، في الداخل
+
+185
+00:13:47,640 --> 00:13:54,180
+الشراينوالأورنا إذا تراكمت دي post-LDL كوليسترول
+
+186
+00:13:54,180 --> 00:13:57,320
+particle in visceral wall بسبب بلاتيوم نسميها
+
+187
+00:13:57,320 --> 00:14:02,540
+الجلطات أو التخسرات انه تراكمت ال LDL كوليسترول في
+
+188
+00:14:02,540 --> 00:14:06,140
+داخل الأوعية الدموية نسميها blood هذه بتسبب
+
+189
+00:14:06,140 --> 00:14:11,400
+inflammation التهابات بتسبب bleeding بتسبب انه
+
+190
+00:14:11,400 --> 00:14:15,280
+يصير عندي calcification تراكم لالكالسيوم في
+
+191
+00:14:15,280 --> 00:14:21,840
+الأوعية الدمويةالجد مجرد ما يتراكم هذا الكوليسترول
+
+192
+00:14:21,840 --> 00:14:26,400
+في الداخل بيعمل بلاتيو و بيصير تجمعات من هذه
+
+193
+00:14:26,400 --> 00:14:29,080
+الجزيئات الصغيرة بتبدأ تدخل الكوليسترول يدخل
+
+194
+00:14:29,080 --> 00:14:33,380
+الليبد يدخل الليبد على الأوعي والشرهين لحد ما تسكر
+
+195
+00:14:33,380 --> 00:14:38,980
+مجرة الدم فهيبطل ال blood flow هيبطل تدفق للدم
+
+196
+00:14:38,980 --> 00:14:44,260
+فتراكم هذا في مجرة الدم و وقف تدفق الدم بنسميه
+
+197
+00:14:44,260 --> 00:14:50,250
+atherocyclosisأثيرو سيكليروسين الأثيرو سيكليروسين
+
+198
+00:14:50,250 --> 00:14:54,110
+هذه بتسبب .. ممكن تسبب heart disease coronary
+
+199
+00:14:54,110 --> 00:14:59,650
+arteries ممكن تسبب chest pain ألم في الصدر وممكن
+
+200
+00:14:59,650 --> 00:15:04,790
+تسبب coronary artery disease اللي هو أمراض الشراين
+
+201
+00:15:04,790 --> 00:15:11,350
+والقلب لو البلاتيو هذه جلطات أجت فيه ووقفت مشجرة
+
+202
+00:15:11,350 --> 00:15:17,350
+القلب إذا أجت فيه الشريان المغذي للقلببصير عندي
+
+203
+00:15:17,350 --> 00:15:24,560
+heart attack اللي هو الذبح القلبيةإذا وقفت المجرة
+
+204
+00:15:24,560 --> 00:15:28,740
+أو الشريان المغضي لل brain للدماغ بيصير عندي
+
+205
+00:15:28,740 --> 00:15:33,560
+stroke سكتة دماغية إذا إذا اتراكمت الدهون في
+
+206
+00:15:33,560 --> 00:15:37,880
+الشريان المغضي للقلب بيصير عندي heart attack وإذا
+
+207
+00:15:37,880 --> 00:15:41,960
+اتراكمت فيه الشريان ووقفت الشريان المغضي للدماغ
+
+208
+00:15:41,960 --> 00:15:46,840
+بيصير عندي stroke سكتة دماغية عشان هيك ال LDL
+
+209
+00:15:46,840 --> 00:15:52,160
+بنسميه bad cholesterol ال cholesterol السيءنطلق
+
+210
+00:15:52,160 --> 00:15:56,520
+على ال LDL bad cholesterol لأنه بيعمل على نقل
+
+211
+00:15:56,520 --> 00:16:00,320
+الدهون من ال liver إلى الجسم وممكن تتراكم هذه
+
+212
+00:16:00,320 --> 00:16:05,160
+الدهون في الجسم و تسبب جلطات أثيرو سيكلروسيز وممكن
+
+213
+00:16:05,160 --> 00:16:09,960
+تسبب سكتة دماغية و heart attack اللي هو الذبحة
+
+214
+00:16:09,960 --> 00:16:14,970
+القلبية أو الصدريةمعملنا هو عبارة عن isolation of
+
+215
+00:16:14,970 --> 00:16:18,010
+cholesterol from egg yolk حكينا مقدمة عن
+
+216
+00:16:18,010 --> 00:16:21,530
+الكوليسترول وعن انه عقل لكن ده بدنا ندخل في الجزء
+
+217
+00:16:21,530 --> 00:16:25,190
+العامل المعمل بيحكي انه بدنا نعزل الكوليسترول من
+
+218
+00:16:25,190 --> 00:16:28,870
+صفار البيض الجد ال egg كلها الهن egg اللي هي ال
+
+219
+00:16:28,870 --> 00:16:32,370
+egg كاملة بتتكون من egg white بياض البيض ومن ال
+
+220
+00:16:32,370 --> 00:16:37,030
+egg yolk اللي هو صفار البيض البيض بيحتوي على
+
+221
+00:16:37,030 --> 00:16:40,790
+مكوناته شوه عبارة عن إيش عبارة عن قلبه من أولبان
+
+222
+00:16:41,280 --> 00:16:45,900
+البيض عبارة عن قلبه من و لبن أما الصفار الصفار
+
+223
+00:16:45,900 --> 00:16:49,420
+البيض هو عبارة عن glycoprotein
+
+224
+00:16:54,080 --> 00:16:58,920
+Lipid حتى هو بيكون sorry نايت تاني الهين إيج الإيج
+
+225
+00:16:58,920 --> 00:17:02,460
+بيضة كاملة عبارة عن بياض البيض والصفار ال egg
+
+226
+00:17:02,460 --> 00:17:05,800
+white و ال egg yolk البياض ال egg white هو عبارة
+
+227
+00:17:05,800 --> 00:17:10,040
+عن ألبوم ان هو عبارة عن بروتين فقط البياض عبارة عن
+
+228
+00:17:10,040 --> 00:17:13,660
+بروتين الألبوم أما ال egg yolk اللي هو صفار البيض
+
+229
+00:17:13,660 --> 00:17:17,400
+هو عبارة عن lipid و glycoprotein عبارة عن بروتينات
+
+230
+00:17:17,400 --> 00:17:22,510
+سكرية مع دهوننجي لل إيجيوك اللي هو صفار البيت إيش
+
+231
+00:17:22,510 --> 00:17:28,450
+مكوناته؟ حكينا جلايكو بروتين و لبن عبارة عن 5% من
+
+232
+00:17:28,450 --> 00:17:34,750
+مكوناته هو كلسترول و 66% جليسرايد و 28% فوسفولبن
+
+233
+00:17:34,750 --> 00:17:39,730
+هي توزيع الدهون الموجودة في صفار البيت منها 5%
+
+234
+00:17:39,730 --> 00:17:42,710
+كلسترول اللي أنا بدي أخده أعزله بدي أسيب باقي
+
+235
+00:17:42,710 --> 00:17:48,610
+المكوناته بس بدي كلسترولالجيتل ايجيوك بيحكي ليه
+
+236
+00:17:48,610 --> 00:17:52,030
+انها غنية في الدهون صفار البيض هو غني بالدهون
+
+237
+00:17:52,030 --> 00:17:55,170
+بيحتوي على كمية عالية من الدهون من أشهر الدهون
+
+238
+00:17:55,170 --> 00:17:59,450
+اللي موجودة فيها هي ال glyceride كوليسترول و
+
+239
+00:17:59,450 --> 00:18:04,410
+phosphatidylcholine او اسمه التاني إليستين اذا
+
+240
+00:18:04,410 --> 00:18:07,690
+صفار البيض يحتوي على الليبد غني بالليبد أشهر
+
+241
+00:18:07,690 --> 00:18:12,920
+الليبد اللي موجودة فيه ال glycerideو الكلسترول
+
+242
+00:18:12,920 --> 00:18:16,320
+فوسفاتيدي الكولين اللي هو لستين احنا نعزل
+
+243
+00:18:16,320 --> 00:18:20,220
+الكلسترول الكلسترول اللي حكينا عنه اللي جتنا نحكي
+
+244
+00:18:20,220 --> 00:18:23,120
+عن فوسفاتيدي الكولين اشي بسيط عن فوسفاتيدي الكولين
+
+245
+00:18:23,120 --> 00:18:27,840
+او لستين نفس الاسم فوسفاتيدي الكولين او لستين هو
+
+246
+00:18:27,840 --> 00:18:32,240
+اشهر phospholipin موجود في الحيوان و النبات
+
+247
+00:18:32,240 --> 00:18:38,080
+فوسفاتيدي الكولين او لستينهو أشهر phospholipid
+
+248
+00:18:38,080 --> 00:18:45,000
+موجود عند الحيوان و النبات وظائف هو له تلت وظائف
+
+249
+00:18:45,000 --> 00:18:48,580
+هو ال key building block of membrane by layer هو
+
+250
+00:18:48,580 --> 00:18:52,760
+التركيبة الأساسية للطبقة الصناعية في ال membrane
+
+251
+00:18:52,760 --> 00:18:57,980
+يدخل في التركيبة الأساسية للممبرين by layer هي أول
+
+252
+00:18:57,980 --> 00:19:03,600
+وظيفة وظيفة تانية هو بشكل نسبة عالية في ال plasma
+
+253
+00:19:03,600 --> 00:19:08,860
+membraneالغلاف لـ Plasma high very high proportion
+
+254
+00:19:08,860 --> 00:19:12,900
+of the outer leaflet of the plasma membrane إذا هو
+
+255
+00:19:12,900 --> 00:19:16,300
+في ال membrane by layer وفيه ال plasma membrane
+
+256
+00:19:16,300 --> 00:19:21,020
+تالت وظيفة أن هو عبارة عن ال phospholipid الموجود
+
+257
+00:19:21,020 --> 00:19:25,450
+في ال lipoproteinإذا فاكرين الـ lipoprotein حكينا
+
+258
+00:19:25,450 --> 00:19:30,530
+إنه هي عبارة عن lipid مع protein مع phospholipid
+
+259
+00:19:30,530 --> 00:19:35,310
+ال phospholipid هاي هي عبارة عن فسفة تديلكولين أو
+
+260
+00:19:35,310 --> 00:19:39,750
+لستين إذا هو تلت وظائف له وظيفة الأولى بيدخل في
+
+261
+00:19:39,750 --> 00:19:43,250
+تركيب ال membrane by layer وظيفة التانية بيدخل في
+
+262
+00:19:43,250 --> 00:19:46,830
+تركيب البلازمة membrane وظيفة التالتة هو ال
+
+263
+00:19:46,830 --> 00:19:52,830
+phospholipid الموجود في ال lipoproteinأكتر فوسفو
+
+264
+00:19:52,830 --> 00:19:56,410
+لأكتر ليبو بروتين يحتوي على فوسفة D الكليين هو
+
+265
+00:19:56,410 --> 00:20:01,510
+الـHDL الليبو بروتين الحكينا فيه منها HDL و LDL و
+
+266
+00:20:01,510 --> 00:20:06,530
+VLDL و الكايلو مايكون تركيبتهم تحتوي على فوسفو
+
+267
+00:20:06,530 --> 00:20:10,290
+ليبد الفوسفو ليبد هذا عبارة عن فوسفة D الكليين مين
+
+268
+00:20:10,290 --> 00:20:13,990
+أكتر واحد يحتوي على فوسفة D الكليين في تركيبه هو
+
+269
+00:20:13,990 --> 00:20:15,270
+الـHDL
+
+270
+00:20:17,640 --> 00:20:21,180
+التجربة إن أنا بدي استخدم صفار البيض hard boiled
+
+271
+00:20:21,180 --> 00:20:25,060
+hen egg بدي أسلك بيضة بدي بيضة مسلوقة و بدي أخد
+
+272
+00:20:25,060 --> 00:20:30,000
+منها الصفار و بدي أستخدم أسيتون water bath flask
+
+273
+00:20:30,000 --> 00:20:38,540
+funnel filter paper و بيكهة ديش نشوف كدهأنا بدي
+
+274
+00:20:38,540 --> 00:20:41,260
+أعزل الكوليسترول من صفار البيض و أحسب ال
+
+275
+00:20:41,260 --> 00:20:47,100
+percentage ال
+
+276
+00:20:47,100 --> 00:20:50,100
+principle بناء على إيش أنا بدي أعزل الكوليسترول و
+
+277
+00:20:50,100 --> 00:20:54,720
+أسيب باقي المكوناتبيحكيلي بناء على ال solubility
+
+278
+00:20:54,720 --> 00:20:58,680
+in different organic solvents لجيت هم طب كل
+
+279
+00:20:58,680 --> 00:21:02,920
+المكونات لبن و كلهم هيدوبوا في نفس الأشياء كيف بدي
+
+280
+00:21:02,920 --> 00:21:05,860
+أخد الكوليسترول و أسيب كل الباقي اللبن بناء على ال
+
+281
+00:21:05,860 --> 00:21:09,760
+solubility؟ بيحكيلي إنه وجدوا أن الكوليسترول هو
+
+282
+00:21:09,760 --> 00:21:13,780
+الوحيد اللي بيكون soluble in أسيتون لما نستخدم
+
+283
+00:21:13,780 --> 00:21:19,450
+أسيتونأحطه على صفار البيض بس الكوليسترول بيدوب
+
+284
+00:21:19,450 --> 00:21:24,190
+والباقي كله بيترسب كل المكونات بتترسب معادا
+
+285
+00:21:24,190 --> 00:21:29,330
+الكوليسترول بيدوب فالمبدأ أني أستخدم أسيتون يعمل
+
+286
+00:21:29,330 --> 00:21:34,610
+على إذابة الكوليسترول فقط وباقي المكونات بتكون
+
+287
+00:21:34,610 --> 00:21:40,790
+insoluble بتكون مترسمة إيش هو الأسيتون؟الاسيتون
+
+288
+00:21:40,790 --> 00:21:45,470
+اسمه الاخر بروبانون بروبانون اسمه الاخر بروبانون
+
+289
+00:21:45,470 --> 00:21:50,650
+هو عبارة عن organ ال compound مركب عضوي له صيغة
+
+290
+00:21:50,650 --> 00:21:56,030
+CH3 لكل اتنين CO هو خصائصه colorless لا لون له
+
+291
+00:21:56,030 --> 00:22:02,490
+volatile متطاير flammable قابل الاشتعال وهو أبسط
+
+292
+00:22:02,490 --> 00:22:07,440
+كيتون هو كيتون يعني إيش صيغة الكيتون؟انه C double
+
+293
+00:22:07,440 --> 00:22:11,660
+bond O وR من جهها وR من جهها هو أبسط كيتون لأن
+
+294
+00:22:11,660 --> 00:22:14,600
+الـR من جهها هي عبارة عن CH3 والـR من الجهة
+
+295
+00:22:14,600 --> 00:22:19,720
+التانية هي CH3 برضه تمام، إذا ال principle بناء
+
+296
+00:22:19,720 --> 00:22:23,460
+على استخدام الاسيتون اللي بيدوب، بيدوّب
+
+297
+00:22:23,460 --> 00:22:30,280
+الكوليسترول وباقى المكونات بتترسم إيش الخطوات؟ بدي
+
+298
+00:22:30,280 --> 00:22:34,890
+أجيب بيضة مسلوبة بدي أشيل عنها البيعهجيب بيضة
+
+299
+00:22:34,890 --> 00:22:37,830
+مسلوجة انا مابديش البيض بدي اتخلص منه و بدي اخد
+
+300
+00:22:37,830 --> 00:22:42,970
+السفارة بدي اخد نص السفارة نص ال egg نص السفارة ال
+
+301
+00:22:42,970 --> 00:22:48,330
+egg و اوزنها بدي اخد نص السفارة اوزنها بعدين بدي
+
+302
+00:22:48,330 --> 00:22:52,150
+اخد احطها ب بيكر و اطحنها اعمل mashed egg mashed
+
+303
+00:22:52,150 --> 00:22:57,130
+يعني اطحنها احط عليها ال acetone خمسة و عشرين مل
+
+304
+00:22:57,130 --> 00:23:01,610
+من ال acetone و بعمل mix لمدة خمس دقايق اذا انا
+
+305
+00:23:01,610 --> 00:23:05,810
+اخدت نص السفارة البيضةوزنتها عشان هتهمني هذه
+
+306
+00:23:05,810 --> 00:23:11,110
+الوزنة في ال percentage في الحسابات بعدين بحطها ب
+
+307
+00:23:11,110 --> 00:23:14,510
+بيكر هذا النص الصفر البيضا بحطها ب بيكر و بطحنها
+
+308
+00:23:14,510 --> 00:23:19,320
+هحط عليها خمسة و عشرين مل من الاسيتونو بعدين اضنني
+
+309
+00:23:19,320 --> 00:23:24,060
+احرك لمدة خمس دقايق بعد خمس دقايق يفترض ال acetone
+
+310
+00:23:24,060 --> 00:23:29,320
+الشمال بيترسب كل المكونات في الراسب و فوق السائل
+
+311
+00:23:29,320 --> 00:23:33,660
+يحتوي على acetone مع كلسترول انا بدي السائل بديش
+
+312
+00:23:33,660 --> 00:23:37,480
+الطبق اللي تحت بدي السائل اللي فوق اللي هو عبارة
+
+313
+00:23:37,480 --> 00:23:41,440
+عن acetone وكلسترول لان كلسترولالوحيد اللي بيدور
+
+314
+00:23:41,440 --> 00:23:45,060
+في الـ Acetone إيش بدي أعمل؟ بدي أسحب .. أحاول
+
+315
+00:23:45,060 --> 00:23:48,980
+أسحب السائل هذا اللي فوق أسحب الطبقة اللي فوق بـ
+
+316
+00:23:48,980 --> 00:23:52,740
+dropper أو بأي شيء بدي أسحب الطبقة اللي فوق السائل
+
+317
+00:23:52,740 --> 00:23:57,660
+و أحطه في بيكر تانيوأسكر عليها عشان مايتطيرش
+
+318
+00:23:57,660 --> 00:24:02,940
+الاسيتون أرجع تاني على الترسب اللي دال عندي في
+
+319
+00:24:02,940 --> 00:24:06,920
+البيكر ال recipe اللي تحت باضيف عليها كمان 25 مل
+
+320
+00:24:06,920 --> 00:24:11,530
+من الاسيتون وأعمل mix أكرر الخطوة اللي جابانأنا
+
+321
+00:24:11,530 --> 00:24:16,310
+جبت نص صفر البيض و طحنته و حطيت عليه 25 مل من
+
+322
+00:24:16,310 --> 00:24:20,650
+الأسيتون و عملت mix هيكون فوق في السائل في عندي
+
+323
+00:24:20,650 --> 00:24:24,470
+أسيتون مع كوليسترول و عندي راسب تحت بدي أخد السائل
+
+324
+00:24:24,470 --> 00:24:28,340
+هذا اللي فوق أنجله على بيكر تانيو بعدين الطبقة
+
+325
+00:24:28,340 --> 00:24:31,520
+اللى تحتها دى عشان اتأكد ان اخد كل الكوليسترول
+
+326
+00:24:31,520 --> 00:24:37,500
+بضيف كمان 25 مل أسيتون و اضلنى أحرق و بعدين اخد
+
+327
+00:24:37,500 --> 00:24:39,760
+السائل اللى فوق اللى هو عبارة عن أسيتون و
+
+328
+00:24:39,760 --> 00:24:43,440
+كوليسترول اضيفه على الجزء الأول على ال beaker اللى
+
+329
+00:24:43,440 --> 00:24:48,360
+نجلته من الجزء الأول بيصير عندى هيك 50 مل 25 مرة
+
+330
+00:24:48,360 --> 00:24:51,700
+الأولى و 25 مرة التانية عبارة عن أسيتون مع
+
+331
+00:24:51,700 --> 00:24:57,530
+كوليسترولهلجيت بديش انا مين؟ عشان اتأكد ان انا
+
+332
+00:24:57,530 --> 00:25:02,410
+مااخدتش راسب بده اعمل فلترة بعد ما نجلت الاسيتون و
+
+333
+00:25:02,410 --> 00:25:05,670
+الكوليسترول بده اعمل فلترة بده اجيب فلوسك و اجيب
+
+334
+00:25:05,670 --> 00:25:11,100
+فيها funnel محجن و filter بيماوأديره في هذا ال
+
+335
+00:25:11,100 --> 00:25:14,260
+filter paper على ال filter paper خلية ي filter
+
+336
+00:25:14,260 --> 00:25:18,840
+هينزل عند السائل ومين هو السائل؟ السائل عبارة عن
+
+337
+00:25:18,840 --> 00:25:22,140
+أسيتون مع كوليسترون أما الراسب اللي بيضال في ال
+
+338
+00:25:22,140 --> 00:25:26,220
+filter paper هذا بدّيش هيانا بدي السائل اللي هو
+
+339
+00:25:26,220 --> 00:25:30,140
+يحتوي على كوليسترول باخد السائل هاد من ال flask
+
+340
+00:25:30,140 --> 00:25:33,760
+بعد ما يخلص فلترة بدي أرمي الورقة و أخد السائل
+
+341
+00:25:33,760 --> 00:25:37,940
+السائل هاد عبارة عن أسيتون مع كوليسترول بديش
+
+342
+00:25:37,940 --> 00:25:41,760
+الأسيتون بدي بس كوليسترول الأسيتون حكينا من خصائصه
+
+343
+00:25:41,760 --> 00:25:46,170
+أنه volatile إنه متطاير تمام؟فاش بدي أسوي بدي أخده
+
+344
+00:25:46,170 --> 00:25:50,470
+في water bath في ميه سخنه بدي أحط ال baker الميه
+
+345
+00:25:50,470 --> 00:25:54,770
+السخنه بتساعد على أنه يطير ال acetone بسرعة بيطير
+
+346
+00:25:54,770 --> 00:25:59,450
+ال acetone و بيضل عندي بس كلسترول باخده و بحطه على
+
+347
+00:25:59,450 --> 00:26:03,630
+تلج على ice عشان يعمل heat shock صدمة حرارية
+
+348
+00:26:03,630 --> 00:26:08,010
+للكلسترول أنه يتجمع مع بعض بيتجمع عندي على شكل
+
+349
+00:26:08,010 --> 00:26:14,390
+crystal الكوليسترول بيتجمع على شكل crystalهذه
+
+350
+00:26:14,390 --> 00:26:18,910
+الكريستال هي عبارة عن الكوليسترول باخدها و بوزنها
+
+351
+00:26:18,910 --> 00:26:22,990
+يعني البيكر بكون وزنها من الأول وزن وزنته وعارف
+
+352
+00:26:22,990 --> 00:26:27,530
+جداش وزنته عشان أنقص بعد هيك الوزنة لإن لما اوزن
+
+353
+00:26:27,530 --> 00:26:30,690
+الكوليسترول في البيكر هيوزنني اتنين مع بعض
+
+354
+00:26:30,690 --> 00:26:35,730
+كوليسترول مع البيكر فانقص الوزنة زي ما عملنا أيام
+
+355
+00:26:35,730 --> 00:26:39,450
+ال كازين لما وزننا الورجة و بعدين وزننا الورجة مع
+
+356
+00:26:39,450 --> 00:26:44,360
+الكازينتمام و باخد وزنة الكوليسترول و بدي أحسبله
+
+357
+00:26:44,360 --> 00:26:48,200
+ال percentage ال percentage للكوليسترول إيش بتساوي
+
+358
+00:26:48,200 --> 00:26:51,440
+weight of الكوليسترول وزنة الكوليسترول اللي طلعت
+
+359
+00:26:51,440 --> 00:26:55,480
+معاكي في الآخر اللي وزنتيها على وزن نص البيضة اللي
+
+360
+00:26:55,480 --> 00:27:00,180
+وزنناها في الأول لما حكينا أنه نص صفار اللي أخدناه
+
+361
+00:27:00,180 --> 00:27:06,000
+ضرب 100% و بحسب وزنة الكوليسترول اللي موجودة في
+
+362
+00:27:06,000 --> 00:27:14,050
+الصفار البيضو هيك بيكون خلصنا هذا المعمل و يعطيكم
+
+363
+00:27:14,050 --> 00:27:14,490
+العافية
+
diff --git a/PL9fwy3NUQKwZ1Q7zOg1ObgufPMjRI3WY1/9CmoB4eQs2I_raw.json b/PL9fwy3NUQKwZ1Q7zOg1ObgufPMjRI3WY1/9CmoB4eQs2I_raw.json
new file mode 100644
index 0000000000000000000000000000000000000000..8ad377255ae1d286f6865c7fbf16750c479267e4
--- /dev/null
+++ b/PL9fwy3NUQKwZ1Q7zOg1ObgufPMjRI3WY1/9CmoB4eQs2I_raw.json
@@ -0,0 +1 @@
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البحر، في الدجاج المقلي، في الهامبورجر، في الحليب، في المارجارينة، موجودة في أشياء كتير، موجودة كوليسترون، لكن نسبة اللي بنحصل عليها من الجسم هي فقط 20% في عندنا حاجة اسمها lipoprotein", "tokens": [7649, 995, 6225, 3555, 9640, 3660, 18871, 10874, 3215, 12610, 4724, 1829, 2407, 11242, 5016, 16247, 9566, 25957, 13672, 1829, 39894, 33753, 45865, 15844, 33251, 12610, 1829, 14851, 2288, 11536, 37893, 3714, 16095, 3660, 9122, 2655, 13546, 39896, 3714, 29245, 23328, 3660, 8978, 33251, 3555, 41891, 12399, 3714, 29245, 23328, 3660, 8978, 2423, 21292, 11242, 12399, 3714, 29245, 23328, 3660, 8978, 8608, 2288, 9566, 7649, 29739, 5016, 2288, 12399, 8978, 32748, 7435, 26108, 9673, 4587, 20292, 12399, 8978, 2423, 3224, 10943, 3555, 13063, 7435, 2288, 12399, 8978, 21542, 20292, 3555, 12399, 8978, 9673, 9640, 7435, 9640, 9957, 3660, 12399, 3714, 29245, 23328, 3660, 8978, 5551, 8592, 1829, 16606, 9122, 2655, 13546, 12399, 3714, 29245, 23328, 3660, 9122, 12610, 1829, 14851, 2288, 11536, 12399, 44381, 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"word": " موجودة", "probability": 0.9775390625}, {"start": 238.98, "end": 239.08, "word": " في", "probability": 0.982421875}, {"start": 239.08, "end": 239.54, "word": " سرطان", "probability": 0.9617919921875}, {"start": 239.54, "end": 240.16, "word": " البحر،", "probability": 0.9725341796875}, {"start": 240.16, "end": 240.3, "word": " في", "probability": 0.97705078125}, {"start": 240.3, "end": 240.8, "word": " الدجاج", "probability": 0.9622395833333334}, {"start": 240.8, "end": 241.4, "word": " المقلي،", "probability": 0.932861328125}, {"start": 241.4, "end": 241.46, "word": " في", "probability": 0.970703125}, {"start": 241.46, "end": 242.26, "word": " الهامبورجر،", "probability": 0.79840087890625}, {"start": 242.26, "end": 242.34, "word": " في", "probability": 0.94873046875}, {"start": 242.34, "end": 243.3, "word": " الحليب،", "probability": 0.9864501953125}, {"start": 243.3, "end": 243.38, "word": " في", "probability": 0.9267578125}, {"start": 243.38, "end": 244.64, "word": " المارجارينة،", "probability": 0.6994280133928571}, {"start": 244.64, "end": 245.36, "word": " موجودة", "probability": 0.9586181640625}, {"start": 245.36, "end": 245.42, "word": " في", "probability": 0.98388671875}, {"start": 245.42, "end": 245.76, "word": " أشياء", "probability": 0.981201171875}, {"start": 245.76, "end": 246.12, "word": " كتير،", "probability": 0.8795166015625}, {"start": 246.12, "end": 246.42, "word": " موجودة", "probability": 0.972412109375}, {"start": 246.42, "end": 246.96, "word": " كوليسترون،", "probability": 0.8527483258928571}, {"start": 246.96, "end": 247.14, "word": " لكن", "probability": 0.9814453125}, {"start": 247.14, "end": 247.6, "word": " نسبة", "probability": 0.817626953125}, {"start": 247.6, "end": 247.72, "word": " اللي", "probability": 0.9072265625}, {"start": 247.72, "end": 248.04, "word": " بنحصل", "probability": 0.9630533854166666}, {"start": 248.04, "end": 248.24, "word": " عليها", "probability": 0.99267578125}, {"start": 248.24, "end": 248.36, "word": " من", "probability": 0.92724609375}, {"start": 248.36, "end": 248.74, "word": " الجسم", "probability": 0.989990234375}, {"start": 248.74, "end": 248.94, "word": " هي", "probability": 0.9560546875}, {"start": 248.94, "end": 249.5, "word": " فقط", "probability": 0.990966796875}, {"start": 249.5, "end": 250.56, "word": " 20", "probability": 0.5322265625}, {"start": 250.56, "end": 251.7, "word": "%", "probability": 0.9423828125}, {"start": 251.7, "end": 253.68, "word": " في", "probability": 0.5322265625}, {"start": 253.68, "end": 254.04, "word": " عندنا", "probability": 0.57647705078125}, {"start": 254.04, "end": 254.34, "word": " حاجة", "probability": 0.9957682291666666}, {"start": 254.34, "end": 254.82, "word": " اسمها", "probability": 0.92626953125}, {"start": 254.82, "end": 256.04, "word": " lipoprotein", "probability": 0.7660888671875}], "temperature": 1.0}, {"id": 12, "seek": 28387, "start": 256.67, "end": 283.87, "text": "الـ lipoprotein إيش هي؟ هلجيت اللبد هو non-polar 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اللي", "probability": 0.9921875}, {"start": 326.15, "end": 326.29, "word": " هو", "probability": 0.96435546875}, {"start": 326.29, "end": 326.33, "word": " ال", "probability": 0.7822265625}, {"start": 326.33, "end": 326.99, "word": " lipoprotein", "probability": 0.78203125}, {"start": 326.99, "end": 327.39, "word": " متصل", "probability": 0.962646484375}, {"start": 327.39, "end": 327.71, "word": " معه", "probability": 0.7098388671875}, {"start": 327.71, "end": 328.07, "word": " lipid", "probability": 0.802490234375}, {"start": 328.07, "end": 328.21, "word": " و", "probability": 0.9248046875}, {"start": 328.21, "end": 328.73, "word": " protein", "probability": 0.892578125}, {"start": 328.73, "end": 329.03, "word": " و", "probability": 0.9560546875}, {"start": 329.03, "end": 329.87, "word": " phospholipid", "probability": 0.9345703125}, {"start": 329.87, "end": 330.71, "word": " بيكون", "probability": 0.9690755208333334}, {"start": 330.71, "end": 331.31, "word": " soluble", 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density lipoprotein اللي هو VLDL وفي أن ال low density lipoprotein اللي هو LDL", "tokens": [33456, 14407, 3615, 2423, 39184, 8280, 404, 81, 1370, 259, 9673, 29245, 23328, 3660, 8978, 14739, 14739, 14407, 3615, 6055, 4587, 38251, 2655, 11331, 35457, 33251, 12984, 31845, 3660, 11331, 35457, 9673, 4117, 2407, 8315, 2655, 4032, 5016, 35457, 2423, 2407, 19913, 33911, 3660, 11331, 35457, 2423, 10305, 12686, 2445, 9122, 12984, 31845, 3660, 4032, 4117, 2407, 8315, 2655, 4032, 2407, 19913, 33911, 3660, 9154, 29538, 14739, 14407, 3615, 2423, 4386, 14739, 14407, 3615, 2423, 8280, 404, 81, 1370, 259, 8978, 9154, 3224, 9122, 995, 26895, 2407, 19446, 1829, 37983, 11536, 4032, 41185, 14739, 2423, 588, 2295, 10305, 8280, 404, 81, 1370, 259, 13672, 1829, 31439, 691, 23704, 43, 4032, 41185, 14739, 2423, 2295, 10305, 8280, 404, 81, 1370, 259, 13672, 1829, 31439, 33936, 43], "avg_logprob": -0.16493056087739885, "compression_ratio": 2.088785046728972, "no_speech_prob": 7.152557373046875e-07, "words": 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lipoprotein كيف موظيفته؟ بيعمل على نقل الدهون من الخلايا الذائد الدهون اللي بيكون ذائد في الجسم هالجت وزعنا دهون على خلايا الجسم", "tokens": [10721, 15042, 2423, 6920, 8280, 327, 13672, 1829, 8978, 3224, 2423, 15019, 13672, 1829, 4724, 1829, 9381, 1863, 5016, 11296, 2423, 15019, 47524, 19986, 12610, 18871, 11296, 2423, 33936, 43, 4032, 691, 23704, 43, 4032, 12149, 43, 28242, 36764, 24401, 3660, 4032, 19913, 33911, 47395, 2423, 12149, 43, 2423, 1090, 10305, 8280, 404, 81, 1370, 259, 9122, 33911, 3714, 2407, 19913, 33911, 47395, 22807, 4724, 1829, 25957, 1211, 15844, 8717, 4587, 1211, 32748, 3224, 11536, 9154, 33962, 15040, 25528, 32545, 16373, 3215, 32748, 3224, 11536, 13672, 1829, 4724, 1829, 30544, 29910, 16373, 3215, 8978, 25724, 38251, 8032, 6027, 7435, 2655, 4032, 11622, 3615, 8315, 11778, 3224, 11536, 15844, 16490, 15040, 25528, 25724, 38251], "avg_logprob": -0.19133771420047993, "compression_ratio": 1.8114035087719298, "no_speech_prob": 2.384185791015625e-07, "words": [{"start": 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الجسم التوازنة", "tokens": [1211, 10721, 2423, 39184, 12149, 43, 7251, 25957, 1211, 15844, 8717, 4587, 1211, 33251, 12610, 1829, 14851, 2288, 12610, 9154, 16490, 15040, 25528, 25724, 38251, 4724, 47341, 3615, 6055, 7649, 1829, 15844, 13672, 33911, 2288, 2423, 39184, 33936, 43, 4032, 2423, 39184, 691, 23704, 43, 6055, 4587, 20498, 20666, 1863, 4587, 1211, 13672, 1829, 44510, 9154, 13672, 33911, 2288, 30731, 16490, 15040, 25528, 25724, 38251, 16712, 2407, 31377, 1863, 3660], "avg_logprob": -0.18320863305682866, "compression_ratio": 1.7430555555555556, "no_speech_prob": 1.3113021850585938e-06, "words": [{"start": 510.99, "end": 511.31, "word": "لأ", "probability": 0.98095703125}, {"start": 511.31, "end": 511.51, "word": " الـ", "probability": 0.8857421875}, {"start": 511.51, "end": 511.99, "word": " HDL", "probability": 0.783447265625}, {"start": 511.99, "end": 516.17, "word": " يعمل", "probability": 0.6533203125}, {"start": 516.17, "end": 516.45, "word": " على", "probability": 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{"start": 1268.68, "end": 1269.52, "word": " الكوليسترول", "probability": 0.9715169270833334}, {"start": 1269.52, "end": 1269.76, "word": " هو", "probability": 0.994140625}, {"start": 1269.76, "end": 1270.6, "word": " الوحيد", "probability": 0.97216796875}, {"start": 1270.6, "end": 1270.78, "word": " اللي", "probability": 0.96875}, {"start": 1270.78, "end": 1271.12, "word": " بيكون", "probability": 0.9607747395833334}, {"start": 1271.12, "end": 1271.58, "word": " soluble", "probability": 0.901123046875}, {"start": 1271.58, "end": 1271.78, "word": " in", "probability": 0.9267578125}, {"start": 1271.78, "end": 1272.34, "word": " أسيتون", "probability": 0.789794921875}, {"start": 1272.34, "end": 1273.2, "word": " لما", "probability": 0.865234375}, {"start": 1273.2, "end": 1273.78, "word": " نستخدم", "probability": 0.99267578125}, {"start": 1273.78, "end": 1274.52, "word": " أسيتون", "probability": 0.9808349609375}], "temperature": 1.0}, {"id": 56, "seek": 130007, "start": 1274.85, "end": 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recipe اللي تحت باضيف عليها كمان 25 مل من الاسيتون وأعمل mix أكرر الخطوة اللي جابان", "tokens": [2407, 10721, 3794, 37983, 25894, 11296, 6225, 8592, 7649, 19446, 36081, 9566, 13546, 8592, 2423, 32277, 36081, 11536, 5551, 47341, 3615, 6055, 7649, 1829, 15844, 16712, 2288, 3794, 3555, 13672, 1829, 11778, 6027, 18871, 16254, 8978, 2423, 21292, 37983, 2423, 6782, 13672, 1829, 6055, 33753, 4724, 46958, 33911, 25894, 11296, 9122, 2304, 7649, 3552, 3714, 1211, 9154, 2423, 32277, 36081, 11536, 36725, 25957, 1211, 2890, 5551, 37983, 2288, 33962, 9566, 2407, 3660, 13672, 1829, 10874, 16758, 7649], "avg_logprob": -0.3086939121668155, "compression_ratio": 1.6724137931034482, "no_speech_prob": 5.960464477539062e-07, "words": [{"start": 1435.64, "end": 1436.3, "word": "وأسكر", "probability": 0.3853759765625}, {"start": 1436.3, "end": 1436.72, "word": " عليها", "probability": 0.61572265625}, {"start": 1436.72, "end": 1437.02, "word": " عشان", "probability": 0.9332682291666666}, {"start": 1437.02, 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"probability": 0.99072265625}, {"start": 1590.41, "end": 1590.69, "word": " بعض", "probability": 0.99267578125}, {"start": 1590.69, "end": 1591.59, "word": " كوليسترول", "probability": 0.9231770833333334}, {"start": 1591.59, "end": 1592.31, "word": " مع", "probability": 0.9599609375}, {"start": 1592.31, "end": 1593.33, "word": " البيكر", "probability": 0.9332682291666666}, {"start": 1593.33, "end": 1594.03, "word": " فانقص", "probability": 0.8184814453125}, {"start": 1594.03, "end": 1594.73, "word": " الوزنة", "probability": 0.981640625}, {"start": 1594.73, "end": 1594.93, "word": " زي", "probability": 0.943359375}, {"start": 1594.93, "end": 1595.05, "word": " ما", "probability": 0.9775390625}, {"start": 1595.05, "end": 1595.43, "word": " عملنا", "probability": 0.9601236979166666}, {"start": 1595.43, "end": 1595.73, "word": " أيام", "probability": 0.834228515625}, {"start": 1595.73, "end": 1595.91, "word": " ال", "probability": 0.71533203125}, {"start": 1595.91, "end": 1596.37, "word": " كازين", "probability": 0.6437174479166666}, {"start": 1596.37, "end": 1596.93, "word": " لما", "probability": 0.974609375}, {"start": 1596.93, "end": 1597.43, "word": " وزننا", "probability": 0.9459228515625}, {"start": 1597.43, "end": 1597.89, "word": " الورجة", "probability": 0.8870849609375}, {"start": 1597.89, "end": 1598.05, "word": " و", "probability": 0.8115234375}, {"start": 1598.05, "end": 1598.35, "word": " بعدين", "probability": 0.9091796875}, {"start": 1598.35, "end": 1598.81, "word": " وزننا", "probability": 0.970703125}, {"start": 1598.81, "end": 1599.29, "word": " الورجة", "probability": 0.9921875}, {"start": 1599.29, "end": 1599.45, "word": " مع", "probability": 0.9931640625}, {"start": 1599.45, "end": 1600.05, "word": " الكازين", "probability": 0.8846028645833334}], "temperature": 1.0}, {"id": 71, "seek": 162820, "start": 1601.0, "end": 1628.2, "text": "تمام و باخد وزنة الكوليسترول و بدي أحسبله ال percentage ال percentage للكوليسترول إيش بتساوي weight of الكوليسترول وزنة الكوليسترول اللي طلعت معاكي في الآخر اللي وزنتيها على وزن نص البيضة اللي وزنناها في الأول لما حكينا أنه نص صفار اللي أخدناه ضرب 100% و بحسب وزنة الكوليسترول اللي موجودة في الصفار البيض", "tokens": [39237, 10943, 4032, 4724, 47283, 3215, 4032, 11622, 1863, 3660, 33251, 12610, 1829, 14851, 2288, 12610, 4032, 4724, 16254, 5551, 5016, 35457, 43761, 2423, 9668, 2423, 9668, 5296, 23275, 12610, 1829, 14851, 2288, 12610, 11933, 1829, 8592, 39894, 3794, 995, 45865, 3364, 295, 33251, 12610, 1829, 14851, 2288, 12610, 4032, 11622, 1863, 3660, 33251, 12610, 1829, 14851, 2288, 12610, 13672, 1829, 23032, 1211, 34268, 20449, 995, 4117, 1829, 8978, 6024, 95, 34740, 13672, 1829, 4032, 11622, 29399, 1829, 11296, 15844, 4032, 11622, 1863, 8717, 9381, 2423, 21292, 11242, 3660, 13672, 1829, 4032, 11622, 1863, 8315, 11296, 8978, 16247, 12610, 5296, 15042, 11331, 4117, 1829, 8315, 14739, 3224, 8717, 9381, 20328, 5172, 9640, 13672, 1829, 5551, 9778, 3215, 8315, 3224, 48812, 25513, 2319, 4, 4032, 4724, 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"word": " ال", "probability": 0.92578125}, {"start": 1604.68, "end": 1605.24, "word": " percentage", "probability": 0.80517578125}, {"start": 1605.24, "end": 1606.08, "word": " ال", "probability": 0.953125}, {"start": 1606.08, "end": 1606.58, "word": " percentage", "probability": 0.90869140625}, {"start": 1606.58, "end": 1607.38, "word": " للكوليسترول", "probability": 0.8755580357142857}, {"start": 1607.38, "end": 1607.58, "word": " إيش", "probability": 0.8362630208333334}, {"start": 1607.58, "end": 1608.2, "word": " بتساوي", "probability": 0.96875}, {"start": 1608.2, "end": 1608.96, "word": " weight", "probability": 0.91552734375}, {"start": 1608.96, "end": 1609.2, "word": " of", "probability": 0.97509765625}, {"start": 1609.2, "end": 1609.9, "word": " الكوليسترول", "probability": 0.8938802083333334}, {"start": 1609.9, "end": 1610.36, "word": " وزنة", "probability": 0.8438720703125}, {"start": 1610.36, "end": 1610.98, "word": " الكوليسترول", "probability": 0.9701334635416666}, {"start": 1610.98, "end": 1611.14, "word": " اللي", "probability": 0.780517578125}, {"start": 1611.14, "end": 1611.44, "word": " طلعت", "probability": 0.9881184895833334}, {"start": 1611.44, "end": 1611.78, "word": " معاكي", "probability": 0.945556640625}, {"start": 1611.78, "end": 1611.86, "word": " في", "probability": 0.955078125}, {"start": 1611.86, "end": 1612.22, "word": " الآخر", "probability": 0.9697265625}, {"start": 1612.22, "end": 1612.38, "word": " اللي", "probability": 0.96630859375}, {"start": 1612.38, "end": 1613.08, "word": " وزنتيها", "probability": 0.9748046875}, {"start": 1613.08, "end": 1613.96, "word": " على", "probability": 0.79248046875}, {"start": 1613.96, "end": 1614.42, "word": " وزن", "probability": 0.9734700520833334}, {"start": 1614.42, "end": 1614.82, "word": " نص", "probability": 0.8046875}, {"start": 1614.82, "end": 1615.36, "word": " البيضة", "probability": 0.86669921875}, {"start": 1615.36, "end": 1615.48, "word": " اللي", "probability": 0.978515625}, {"start": 1615.48, "end": 1615.98, "word": " وزنناها", "probability": 0.91533203125}, {"start": 1615.98, "end": 1616.08, "word": " في", "probability": 0.96630859375}, {"start": 1616.08, "end": 1616.44, "word": " الأول", "probability": 0.959716796875}, {"start": 1616.44, "end": 1616.68, "word": " لما", "probability": 0.953369140625}, {"start": 1616.68, "end": 1617.1, "word": " حكينا", "probability": 0.9625244140625}, {"start": 1617.1, "end": 1617.3, "word": " أنه", "probability": 0.6978759765625}, {"start": 1617.3, "end": 1617.6, "word": " نص", "probability": 0.988525390625}, {"start": 1617.6, "end": 1618.8, "word": " صفار", "probability": 0.7178141276041666}, {"start": 1618.8, "end": 1618.98, "word": " اللي", "probability": 0.986572265625}, {"start": 1618.98, "end": 1620.18, "word": " أخدناه", "probability": 0.88115234375}, {"start": 1620.18, "end": 1620.52, "word": " ضرب", "probability": 0.837890625}, {"start": 1620.52, "end": 1620.96, "word": " 100", "probability": 0.80224609375}, {"start": 1620.96, "end": 1621.68, "word": "%", "probability": 0.95654296875}, {"start": 1621.68, "end": 1622.24, "word": " و", "probability": 0.794921875}, {"start": 1622.24, "end": 1622.78, "word": " بحسب", "probability": 0.8473307291666666}, {"start": 1622.78, "end": 1623.34, "word": " وزنة", "probability": 0.9254150390625}, {"start": 1623.34, "end": 1624.16, "word": " الكوليسترول", "probability": 0.981689453125}, {"start": 1624.16, "end": 1624.38, "word": " اللي", "probability": 0.7283935546875}, {"start": 1624.38, "end": 1625.68, "word": " موجودة", "probability": 0.98583984375}, {"start": 1625.68, "end": 1626.0, "word": " في", "probability": 0.9921875}, {"start": 1626.0, "end": 1627.66, "word": " الصفار", "probability": 0.9220377604166666}, {"start": 1627.66, "end": 1628.2, "word": " البيض", "probability": 0.8147786458333334}], "temperature": 1.0}, {"id": 72, "seek": 163449, "start": 1629.89, "end": 1634.49, "text": "و هيك بيكون خلصنا هذا المعمل و يعطيكم العافية", "tokens": [2407, 39896, 4117, 4724, 1829, 30544, 16490, 1211, 9381, 8315, 23758, 9673, 25957, 1211, 4032, 37495, 9566, 1829, 24793, 18863, 31845, 10632], "avg_logprob": -0.12559442805207294, "compression_ratio": 1.1549295774647887, "no_speech_prob": 0.0, "words": [{"start": 1629.89, "end": 1630.09, "word": "و", "probability": 0.873046875}, {"start": 1630.09, "end": 1630.41, "word": " هيك", "probability": 0.84814453125}, {"start": 1630.41, "end": 1630.95, "word": " بيكون", "probability": 0.9755859375}, {"start": 1630.95, "end": 1631.73, "word": " خلصنا", "probability": 0.9742431640625}, {"start": 1631.73, "end": 1632.17, "word": " هذا", "probability": 0.4833984375}, {"start": 1632.17, "end": 1632.97, "word": " المعمل", "probability": 0.9812825520833334}, {"start": 1632.97, "end": 1633.61, "word": " و", "probability": 0.412353515625}, {"start": 1633.61, "end": 1634.05, "word": " يعطيكم", "probability": 0.9090576171875}, {"start": 1634.05, "end": 1634.49, "word": " العافية", "probability": 0.9597981770833334}], "temperature": 1.0}], "language": "ar", "language_probability": 1.0, "duration": 1635.9386875, "duration_after_vad": 1587.0472187499968}
\ No newline at end of file
diff --git a/PL9fwy3NUQKwZ1Q7zOg1ObgufPMjRI3WY1/9CmoB4eQs2I_raw.srt b/PL9fwy3NUQKwZ1Q7zOg1ObgufPMjRI3WY1/9CmoB4eQs2I_raw.srt
new file mode 100644
index 0000000000000000000000000000000000000000..850cda3b1dfe77ebbd2125ed0cfd0e2dded58261
--- /dev/null
+++ b/PL9fwy3NUQKwZ1Q7zOg1ObgufPMjRI3WY1/9CmoB4eQs2I_raw.srt
@@ -0,0 +1,1452 @@
+1
+00:00:00,000 --> 00:00:05,280
+كيفي موضوع جديد هو isolation of cholesterol from
+
+2
+00:00:05,280 --> 00:00:11,100
+egg yolk عزل الكوليسترول من صفار البيضة، نجيب صفار
+
+3
+00:00:11,100 --> 00:00:16,450
+بيضة و نعزل منها الكوليسترولبحكي اليوم الحاجة ان
+
+4
+00:00:16,450 --> 00:00:19,970
+اتعرف على الكلسترول الكلسترول هو most abundant
+
+5
+00:00:19,970 --> 00:00:25,290
+steroid in the body هو أشهر الكلسترول هو أشهر
+
+6
+00:00:25,290 --> 00:00:29,870
+steroid أشهر libid موجود في الجسم أشهر libid موجود
+
+7
+00:00:29,870 --> 00:00:35,110
+في الجسم هو الكلسترول is a waxy substance found in
+
+8
+00:00:35,110 --> 00:00:40,690
+all of the body cell هو عبارة عن مادة شمعية قوامه
+
+9
+00:00:40,690 --> 00:00:47,020
+شمعيموجود في كل أجزاء الجسم كيف تركبته؟ تركبته
+
+10
+00:00:47,020 --> 00:00:53,040
+عبارة عن Methyl group اللي هي CH3 مع Alkyl Chain
+
+11
+00:00:53,040 --> 00:00:59,240
+اللي هي سلسلة منطبط فيها CH3 و OH و Steroid
+
+12
+00:00:59,240 --> 00:01:02,860
+nucleus Steroid nucleus هو عبارة عن الأربع حلقات
+
+13
+00:01:02,860 --> 00:01:08,480
+اللي تحت متصل فيهم OH و سلسلة بنسميها Alkyl Chainو
+
+14
+00:01:08,480 --> 00:01:13,940
+CH3 مرتبطة في هذه السلسلة هذه عبارة عن تركيبة
+
+15
+00:01:13,940 --> 00:01:20,050
+الكوليسترول الـ Structure Aإيش وظائف الكلسترول ال
+
+16
+00:01:20,050 --> 00:01:23,310
+function؟ أول وظيفة help and maintain cell
+
+17
+00:01:23,310 --> 00:01:28,230
+membrane يعمل على الحفاظ و حماية ال cell membrane
+
+18
+00:01:28,230 --> 00:01:33,230
+بيحمي و يحافظ على ال cell membrane وظيفة تانية
+
+19
+00:01:33,230 --> 00:01:37,890
+protect and insulate nerve fiber بيعمل على حماية و
+
+20
+00:01:37,890 --> 00:01:43,910
+عزل الألياف العصبية حماية و عزل الألياف العصبية
+
+21
+00:01:45,300 --> 00:01:49,020
+تلاتة involved in the formation of sex hormones زي
+
+22
+00:01:49,020 --> 00:01:54,040
+الاستروجين يدخل في تركيب ال sex hormones الهرمونات
+
+23
+00:01:54,040 --> 00:01:58,620
+الجنسية زي الاستروجين بيدخل في تركيبها الكوليسترون
+
+24
+00:01:58,620 --> 00:02:02,800
+أربع production of bile salt which help digesting
+
+25
+00:02:02,800 --> 00:02:07,100
+fat زي ما حكينا المعمل اللي فات هو يدخل في تصنيع
+
+26
+00:02:07,100 --> 00:02:10,900
+ال bile salt اللي هي أملاح العصارة السفراوية التي
+
+27
+00:02:10,900 --> 00:02:17,840
+تساعد على هضم الدهونحكينا عنها المعمل اللي فات كيف
+
+28
+00:02:17,840 --> 00:02:21,240
+الكوليسترول موجود في الجسم؟ هلجيت الكوليسترول من
+
+29
+00:02:21,240 --> 00:02:25,960
+أين يحصل عليه الجسم؟ 80% من الكوليسترول بتصنع في
+
+30
+00:02:25,960 --> 00:02:31,460
+ال liver sensitized in the liver بالنسبة 80% و 20%
+
+31
+00:02:31,460 --> 00:02:36,220
+بناخدها من الواجبات موجودة في الحليب، في البيض، في
+
+32
+00:02:36,220 --> 00:02:43,280
+اللحمة، 20% أما 80% بيصنع الجسم في الكبتليش بيحتاج
+
+33
+00:02:43,280 --> 00:02:46,480
+الجسم للكوليسترول بناء على وضعفه بيحتاج لل cell
+
+34
+00:02:46,480 --> 00:02:49,480
+membrane لحمية ال cell membrane لعزل ال nerve
+
+35
+00:02:49,480 --> 00:02:53,720
+tissue العزل الألياف العصبية في تصنيع ال steroid
+
+36
+00:02:53,720 --> 00:02:57,960
+hormone في تصنيع vitamin D هدول كل استخدامات
+
+37
+00:02:57,960 --> 00:02:59,120
+للكوليسترول
+
+38
+00:03:01,080 --> 00:03:05,520
+الكلسترول النسبة الطبيعية له في الجسم أن يكون أقل
+
+39
+00:03:05,520 --> 00:03:10,240
+من 200 ميلي جرام per deciliter كلسترول النسبة
+
+40
+00:03:10,240 --> 00:03:14,600
+الطبيعية له في الجسم أن يكون أقل من 200 ميلي جرام
+
+41
+00:03:14,600 --> 00:03:20,400
+per deciliter إذا زاد elevated plasma cholesterol
+
+42
+00:03:20,400 --> 00:03:23,820
+exceeds 200 ميلي جرام per deciliter زاد عن 200
+
+43
+00:03:23,820 --> 00:03:26,940
+ميلي جرام per deciliter بنسمي هذه الحالة
+
+44
+00:03:26,940 --> 00:03:31,410
+كلسترليمياالـ Cholestrolimia معناها ارتفاع
+
+45
+00:03:31,410 --> 00:03:36,350
+الكوليسترول أكتر من 200 ميلي جرام بر دي سيلتان
+
+46
+00:03:36,350 --> 00:03:40,170
+وهذا إذا ارتفع بيصير اندي clots, arteries وانهي
+
+47
+00:03:40,170 --> 00:03:44,950
+level 4 مبلاتين بيصير انسداد في الشرايين والأوردة
+
+48
+00:03:44,950 --> 00:03:46,730
+وممكن تسبب جلطات
+
+49
+00:03:50,000 --> 00:03:53,240
+انا عبارة عن جدول بيوضح الأطعم اللي بتحتوي على
+
+50
+00:03:53,240 --> 00:03:57,280
+الكوليسترون مش مهمة كتير هي موجودة في الكبدة،
+
+51
+00:03:57,280 --> 00:04:00,800
+موجودة في البيض، موجودة في سرطان البحر، في الدجاج
+
+52
+00:04:00,800 --> 00:04:04,640
+المقلي، في الهامبورجر، في الحليب، في المارجارينة،
+
+53
+00:04:04,640 --> 00:04:07,600
+موجودة في أشياء كتير، موجودة كوليسترون، لكن نسبة
+
+54
+00:04:07,600 --> 00:04:14,340
+اللي بنحصل عليها من الجسم هي فقط 20% في عندنا حاجة
+
+55
+00:04:14,340 --> 00:04:19,130
+اسمها lipoproteinالـ lipoprotein إيش هي؟ هلجيت
+
+56
+00:04:19,130 --> 00:04:23,790
+اللبد هو non-polar بقدرش يدوب في الماء فكيف بده
+
+57
+00:04:23,790 --> 00:04:27,670
+يمشي في الجسم هذا اللبد؟ هلجيت اللبد لأنه non
+
+58
+00:04:27,670 --> 00:04:32,510
+-polar بيحتاج إلى نواقل تنقله في الجسم النواقل هذه
+
+59
+00:04:32,510 --> 00:04:37,730
+عبارة عن lipoprotein ال lipoprotein هي نواقل اللبد
+
+60
+00:04:37,730 --> 00:04:43,150
+هي نواقل لنقل اللبد لأنه اللبد بقدرش يتحرك لأنه
+
+61
+00:04:43,150 --> 00:04:48,580
+non-polarالليبوبروتين هي عبارة عن لبد مع بروتين و
+
+62
+00:04:48,580 --> 00:04:53,480
+عليها كمان فوسفوليبن الليبوبروتين هي عبارة عن لبد
+
+63
+00:04:53,480 --> 00:04:58,040
+مع بروتين و فوسفوليبن اللي جات اللبد لما يتصل على
+
+64
+00:04:58,040 --> 00:05:02,580
+سطحه بروتين و فوسفوليبن بتعطيه خاصية أنه يصير
+
+65
+00:05:02,580 --> 00:05:06,640
+soluble in water لأن ال surface بيكون عبارة عن
+
+66
+00:05:06,640 --> 00:05:10,560
+polar lipid بتكون الخارج السطح المحاط في هذا اللبد
+
+67
+00:05:10,560 --> 00:05:14,250
+هو عبارة عنمواد polar اللي هي ال phospholipid ال
+
+68
+00:05:14,250 --> 00:05:18,870
+phosphatic group بتكون على السطح فبيكون كأنه polar
+
+69
+00:05:18,870 --> 00:05:23,550
+بيكون soluble in water ليش؟ لإتصاله بمجموعة خلته
+
+70
+00:05:23,550 --> 00:05:26,990
+polar زي ال phospholipid اللي هو ال lipoprotein
+
+71
+00:05:26,990 --> 00:05:30,710
+متصل معه lipid و protein و phospholipid بيكون
+
+72
+00:05:30,710 --> 00:05:35,630
+soluble in water لأنه سطحه عبارة عن مجموعات polar
+
+73
+00:05:38,830 --> 00:05:42,970
+أنواع الـ lipoprotein الموجودة في أن أنواع تقسمت
+
+74
+00:05:42,970 --> 00:05:47,310
+حسب الكثافة حسب المكونات وحسب الوظيفة حسب ال
+
+75
+00:05:47,310 --> 00:05:52,490
+density composition function كثافة وكونات ووظيفة
+
+76
+00:05:52,490 --> 00:05:56,790
+من هذه أنواع ال .. أنواع ال lipoprotein في منه
+
+77
+00:05:56,790 --> 00:06:00,790
+كايلو مايكرون وفي أن ال very low density
+
+78
+00:06:00,790 --> 00:06:05,250
+lipoprotein اللي هو VLDL وفي أن ال low density
+
+79
+00:06:05,250 --> 00:06:12,070
+lipoprotein اللي هو LDLو في عندنا ال HDL اللي هو
+
+80
+00:06:12,070 --> 00:06:17,470
+ال high density lipoprotein اذا في عندنا ايش انواع
+
+81
+00:06:17,470 --> 00:06:24,810
+ال lipoprotein في منها كايلو مايكرون VLDL و HDLال
+
+82
+00:06:24,810 --> 00:06:29,390
+kylo micron هو مسئول عن ال external لبد عن اللبد
+
+83
+00:06:29,390 --> 00:06:33,750
+اللي جاي للجسم من برا الجسم مش اللي بيصنع الجسم ال
+
+84
+00:06:33,750 --> 00:06:38,410
+kylo micron مسئول عن الدهون اللي بتيجي من الخارج
+
+85
+00:06:38,410 --> 00:06:43,570
+من الواجبات من الواجبات اليوميةالكايلو مايكرون
+
+86
+00:06:43,570 --> 00:06:48,450
+مسئول عن الـexternal lipid الديوتري لبد توسع اللي
+
+87
+00:06:48,450 --> 00:06:51,930
+هو الدهون اللي جاية من الخارج من خارج الجسم من
+
+88
+00:06:51,930 --> 00:06:55,810
+الواجبات الجسم اتناولها مين المسئول عنها انه يقوم
+
+89
+00:06:55,810 --> 00:07:00,650
+بنقلها هو عبارة عن الكايلو مايكرونالـ Very Low
+
+90
+00:07:00,650 --> 00:07:03,870
+Density Lipoprotein اللي هو الـ VLDL والـ Low
+
+91
+00:07:03,870 --> 00:07:07,030
+Density Lipoprotein الـ LDL وHigh Density
+
+92
+00:07:07,030 --> 00:07:10,310
+Lipoprotein اللي هو الـ HDL هم مسئولين عن الـ
+
+93
+00:07:10,310 --> 00:07:14,970
+Internal Lipid عن اللبد الداخلي اللي بيصنع في
+
+94
+00:07:14,970 --> 00:07:20,620
+الليفانكيف وظائفهم هدول، هذا المخطط بشكل عام أنا
+
+95
+00:07:20,620 --> 00:07:25,780
+ألخصه إنه عندنا الـ Calomicron هي عبارة عن متخصصة
+
+96
+00:07:25,780 --> 00:07:29,600
+عن الـ External Libid اللي جاي من الدياتري، بتيجي
+
+97
+00:07:29,600 --> 00:07:33,300
+دهون اللي الانسان بيتناول واجب الدهون، بتيجي هذه
+
+98
+00:07:33,300 --> 00:07:36,480
+الدهون بتصل للمعدة و الأمعاء، مين بدي ياخدها و
+
+99
+00:07:36,480 --> 00:07:40,060
+يعملها امتصاص و ينقلها؟ هو عبارة عن ال Calomicron
+
+100
+00:07:40,060 --> 00:07:43,940
+مسئول عن ال External Libid اللي جاية من الواجبات،
+
+101
+00:07:43,940 --> 00:07:48,280
+بياخدها، بينقلها، بوزحها على خلايا الجسمأما ال
+
+102
+00:07:48,280 --> 00:07:50,900
+internal lipid اللي فيه ال liver اللي بيصنحها ال
+
+103
+00:07:50,900 --> 00:07:56,440
+liver مسئول عنها ال LDL و VLDL و HDL كل واحدة
+
+104
+00:07:56,440 --> 00:08:00,800
+وظيفته ال HDL ال high density lipoprotein كيف
+
+105
+00:08:00,800 --> 00:08:06,960
+موظيفته؟ بيعمل على نقل الدهون من الخلايا الذائد
+
+106
+00:08:06,960 --> 00:08:10,100
+الدهون اللي بيكون ذائد في الجسم هالجت وزعنا دهون
+
+107
+00:08:10,100 --> 00:08:14,330
+على خلايا الجسمالذي زاد في اتشع و في الـ blood
+
+108
+00:08:14,330 --> 00:08:20,190
+دهون زاد بيجي الـ HDL بيجمع هدول الدهون و بيرجحها
+
+109
+00:08:20,190 --> 00:08:25,510
+تاني على الـ liver لذن الـ HDL carry كلسترول to
+
+110
+00:08:25,510 --> 00:08:31,310
+cell and deposit excess cholesterol in arteryلأ
+
+111
+00:08:31,310 --> 00:08:36,170
+الـ HDL يعمل
+
+112
+00:08:36,170 --> 00:08:41,170
+على نقل الكوليسترول من خلايا الجسم برجع تاني على
+
+113
+00:08:41,170 --> 00:08:47,670
+الليفر الـ LDL و الـ VLDL تقوم بالنقل الليبد من
+
+114
+00:08:47,670 --> 00:08:52,970
+الليفر إلى خلايا الجسم التوازنةالقايلو مايكرو
+
+115
+00:08:52,970 --> 00:08:56,270
+مسئول عن الدهون الخارجية بيوزحها بتيجي الواجبة
+
+116
+00:08:56,270 --> 00:09:01,190
+وبيوزحها وبينقلها إلى خلايا الجسم HDL مسئول على
+
+117
+00:09:01,190 --> 00:09:06,250
+أنه ينقل من خلايا الجسم يرجع تاني على ال liver LDL
+
+118
+00:09:06,250 --> 00:09:12,230
+و VLDL مسئول على أنه يوزع الكوليسترول من ال liver
+
+119
+00:09:12,230 --> 00:09:15,250
+من الكبد يوزع على خلايا الجسم
+
+120
+00:09:18,310 --> 00:09:22,610
+بحكي ليه أن الـ HDL اللي هو الـ high density of
+
+121
+00:09:22,610 --> 00:09:26,730
+the lipoprotein يترك الجلد يتلقى المزيد من
+
+122
+00:09:26,730 --> 00:09:31,090
+الكوليسترول على أسطوله و يتركه إلى الجلد و يتعيد
+
+123
+00:09:31,090 --> 00:09:35,530
+إلى الجلد الـ HDL زي ما حكينا بيغادر من الجلد، هو
+
+124
+00:09:35,530 --> 00:09:39,130
+بيتصنع في الجلد، بيروح و بيطلع برا الجلد و بيروح
+
+125
+00:09:39,130 --> 00:09:42,670
+على خلايا الجسمبيجمع الـ excess cholesterol
+
+126
+00:09:42,670 --> 00:09:46,290
+الكوليسترول الزيادة اللي ضايل في الجسم بيرجعه تاني
+
+127
+00:09:46,290 --> 00:09:49,170
+على ال liver لقيت لما نجيب هدع ال liver إيش بده
+
+128
+00:09:49,170 --> 00:09:52,170
+يسوي فيه ال liver؟ ال liver excrete this
+
+129
+00:09:52,170 --> 00:09:55,650
+cholesterol into the bile then out of the body or
+
+130
+00:09:55,650 --> 00:10:00,250
+used in the bile salt production لقيت ال HDL جاب
+
+131
+00:10:00,250 --> 00:10:03,790
+لل liver جاب من خلايا الجسم كوليسترول إيش بده يعمل
+
+132
+00:10:03,790 --> 00:10:07,910
+فيه ال liver؟ بحكي لل liver إما بيعمل على إخراجه
+
+133
+00:10:07,910 --> 00:10:12,380
+إلى خارج الجسممع الفضلات إخراجه لخارج الجسم أو
+
+134
+00:10:12,380 --> 00:10:16,240
+يستخدموا في تصنيع ال bile salt لأن ال bile salt
+
+135
+00:10:16,240 --> 00:10:20,480
+تصنع من الكوليسترول فهو بيستخدموا في تصنيع هذا ال
+
+136
+00:10:20,480 --> 00:10:23,620
+liver الكوليسترول اللي رجع على ال liver إله طريقيا
+
+137
+00:10:23,620 --> 00:10:29,080
+إما إخراجه خارج الجسم أو تصنيع ال bile salt منه
+
+138
+00:10:29,080 --> 00:10:33,240
+فال HDL لأنه بيعمل على remove excess كوليسترول
+
+139
+00:10:33,240 --> 00:10:38,950
+from body بنسمي protective أو good كوليسترونلذن
+
+140
+00:10:38,950 --> 00:10:44,050
+الـ HDL بيجمع الكوليسترول من خلايا الجسم بيرجحها
+
+141
+00:10:44,050 --> 00:10:47,630
+تاني على ال liver وال liver بيتخلص منها بطريقتين
+
+142
+00:10:47,630 --> 00:10:53,790
+إما بيتخلص منها بإخراجها خارج الجسمأو بعمل تصنيع
+
+143
+00:10:53,790 --> 00:10:58,490
+منها الـ bile salt فلأن الـ HDL جمّع لي الدهون
+
+144
+00:10:58,490 --> 00:11:02,530
+الزيادة في الجسم واتخلص منها بسميه protective
+
+145
+00:11:02,530 --> 00:11:06,290
+وقائي أو good cholesterol اللي هو الكوليسترول
+
+146
+00:11:06,290 --> 00:11:10,390
+الجيد لأنه خلصني من كمية الدهون الزيادة اللي في
+
+147
+00:11:10,390 --> 00:11:14,510
+الجسم لأن احنا زي ما احنا عارفين لو اتراكمت الدهون
+
+148
+00:11:14,510 --> 00:11:18,480
+في الجسمممكن تعمل انسداد في الأوعى الدموية وفي
+
+149
+00:11:18,480 --> 00:11:22,820
+الشرايين وتسبب جلطات فانا إذا اتخلصت منها بيكون
+
+150
+00:11:22,820 --> 00:11:27,780
+أفضل ف الـHDL بيخلصني منها فبسميه protective أو
+
+151
+00:11:27,780 --> 00:11:33,400
+good كوليسترولالـ LDL بيعمل على transport
+
+152
+00:11:33,400 --> 00:11:35,880
+كوليسترول throughout the body delivering
+
+153
+00:11:35,880 --> 00:11:38,820
+cholesterol to different tissue and organs بيعمل
+
+154
+00:11:38,820 --> 00:11:43,540
+على نقل الكوليسترول من الـ liver بيوزع على الأعضاء
+
+155
+00:11:43,540 --> 00:11:47,460
+والأنسجة، على ال tissue و ال organs، بيوزع من
+
+156
+00:11:47,460 --> 00:11:50,760
+الكبد لخلايا الجسمwhen the cell have all
+
+157
+00:11:50,760 --> 00:11:53,240
+cholesterol they need they reduce the number of
+
+158
+00:11:53,240 --> 00:11:55,680
+receptors or gates way of cholesterol to enter
+
+159
+00:11:55,680 --> 00:12:00,160
+الجسم stuck فخلاص صارت وصلته كمية كفاية من ال
+
+160
+00:12:00,160 --> 00:12:06,400
+lipid يفترض انه يحفظ receptor مستقبل او قنوات انه
+
+161
+00:12:06,400 --> 00:12:10,720
+خلاص ال LDLإننا نعطيه إشارة إنه يوقف إنه يبطل يجيب
+
+162
+00:12:10,720 --> 00:12:15,280
+كوليسترول للجسم لأن الجسم أخد حاجته يفترض إنه فيه
+
+163
+00:12:15,280 --> 00:12:20,020
+نيروسيبتر شغال مستقبل يرسل إشارة إنه خلاص ما توصلش
+
+164
+00:12:20,020 --> 00:12:24,440
+كوليسترول تاني للجسم يا LDL وقف ما تشتغلش الجسم
+
+165
+00:12:24,440 --> 00:12:27,960
+عنده كوليسترول بكفايت وما تجيبلهوش تاني as a
+
+166
+00:12:27,960 --> 00:12:31,020
+result كوليسترول contained in the LDL particles
+
+167
+00:12:31,020 --> 00:12:35,230
+start to build up in the bloodstreamإذا هذا الـ
+
+168
+00:12:35,230 --> 00:12:39,550
+Receptor ماشتغلش وكان فيه مشكلة مثلا الـ LDL هيظل
+
+169
+00:12:39,550 --> 00:12:45,150
+مستمر ويعمل على نقل الـ LDL هيظل مستمر وينقل
+
+170
+00:12:45,150 --> 00:12:49,530
+الكوليسترول من الـ liver لخلايا الجسم يظل ينقل،
+
+171
+00:12:49,530 --> 00:12:53,670
+ينقل، طب الجسم استكفى، بدوش كوليسترول، هيظل ينقل،
+
+172
+00:12:53,670 --> 00:12:58,690
+ينقل، ينقل، لحد ما هذه الجزيئات لزيادة تبني نفسها
+
+173
+00:12:58,690 --> 00:13:03,210
+وتستقر في الـ blood stream، في الأوعية الدمويةمع
+
+174
+00:13:03,210 --> 00:13:06,310
+الوجد الكوليسترول هذا اللي بيكون في الأوعية
+
+175
+00:13:06,310 --> 00:13:10,410
+الدموية جاعت بيصير عليه oxidize بيصير عليه عمليات
+
+176
+00:13:10,410 --> 00:13:15,310
+أكسدة عمليات أكسدة يعني يتكسر بيتكسر إلى حجم أصغر
+
+177
+00:13:15,310 --> 00:13:20,110
+reduce inside هلجيت؟ الليبد صار متكون في الأوعية
+
+178
+00:13:20,110 --> 00:13:24,490
+الدموية وحجمه صغير تمام؟ هذه اللي حجمها صغير
+
+179
+00:13:24,490 --> 00:13:26,230
+الليبد اللي حجمها صغير
+
+180
+00:13:28,880 --> 00:13:32,240
+صارت متراكمة في الأوعية الدموية small particles
+
+181
+00:13:32,240 --> 00:13:36,420
+more easily enter the blood vessels فسهل عليها
+
+182
+00:13:36,420 --> 00:13:40,060
+أنها تدخل أي وعاء دموي، أي شريان، أي وريد، لأنها
+
+183
+00:13:40,060 --> 00:13:43,760
+صارت صغيرة، حجمها صغير وبتقدر تدخل على الشراين
+
+184
+00:13:43,760 --> 00:13:47,640
+والأوردة، بتبدأ تتراكم في الداخل، في الداخل
+
+185
+00:13:47,640 --> 00:13:54,180
+الشراينوالأورنا إذا تراكمت دي post-LDL كوليسترول
+
+186
+00:13:54,180 --> 00:13:57,320
+particle in visceral wall بسبب بلاتيوم نسميها
+
+187
+00:13:57,320 --> 00:14:02,540
+الجلطات أو التخسرات انه تراكمت ال LDL كوليسترول في
+
+188
+00:14:02,540 --> 00:14:06,140
+داخل الأوعية الدموية نسميها blood هذه بتسبب
+
+189
+00:14:06,140 --> 00:14:11,400
+inflammation التهابات بتسبب bleeding بتسبب انه
+
+190
+00:14:11,400 --> 00:14:15,280
+يصير عندي calcification تراكم لالكالسيوم في
+
+191
+00:14:15,280 --> 00:14:21,840
+الأوعية الدمويةالجد مجرد ما يتراكم هذا الكوليسترول
+
+192
+00:14:21,840 --> 00:14:26,400
+في الداخل بيعمل بلاتيو و بيصير تجمعات من هذه
+
+193
+00:14:26,400 --> 00:14:29,080
+الجزيئات الصغيرة بتبدأ تدخل الكوليسترول يدخل
+
+194
+00:14:29,080 --> 00:14:33,380
+الليبد يدخل الليبد على الأوعي والشرهين لحد ما تسكر
+
+195
+00:14:33,380 --> 00:14:38,980
+مجرة الدم فهيبطل ال blood flow هيبطل تدفق للدم
+
+196
+00:14:38,980 --> 00:14:44,260
+فتراكم هذا في مجرة الدم و وقف تدفق الدم بنسميه
+
+197
+00:14:44,260 --> 00:14:50,250
+atherocyclosisأثيرو سيكليروسين الأثيرو سيكليروسين
+
+198
+00:14:50,250 --> 00:14:54,110
+هذه بتسبب .. ممكن تسبب heart disease coronary
+
+199
+00:14:54,110 --> 00:14:59,650
+arteries ممكن تسبب chest pain ألم في الصدر وممكن
+
+200
+00:14:59,650 --> 00:15:04,790
+تسبب coronary artery disease اللي هو أمراض الشراين
+
+201
+00:15:04,790 --> 00:15:11,350
+والقلب لو البلاتيو هذه جلطات أجت فيه ووقفت مشجرة
+
+202
+00:15:11,350 --> 00:15:17,350
+القلب إذا أجت فيه الشريان المغذي للقلببصير عندي
+
+203
+00:15:17,350 --> 00:15:24,560
+heart attack اللي هو الذبح القلبيةإذا وقفت المجرة
+
+204
+00:15:24,560 --> 00:15:28,740
+أو الشريان المغضي لل brain للدماغ بيصير عندي
+
+205
+00:15:28,740 --> 00:15:33,560
+stroke سكتة دماغية إذا إذا اتراكمت الدهون في
+
+206
+00:15:33,560 --> 00:15:37,880
+الشريان المغضي للقلب بيصير عندي heart attack وإذا
+
+207
+00:15:37,880 --> 00:15:41,960
+اتراكمت فيه الشريان ووقفت الشريان المغضي للدماغ
+
+208
+00:15:41,960 --> 00:15:46,840
+بيصير عندي stroke سكتة دماغية عشان هيك ال LDL
+
+209
+00:15:46,840 --> 00:15:52,160
+بنسميه bad cholesterol ال cholesterol السيءنطلق
+
+210
+00:15:52,160 --> 00:15:56,520
+على ال LDL bad cholesterol لأنه بيعمل على نقل
+
+211
+00:15:56,520 --> 00:16:00,320
+الدهون من ال liver إلى الجسم وممكن تتراكم هذه
+
+212
+00:16:00,320 --> 00:16:05,160
+الدهون في الجسم و تسبب جلطات أثيرو سيكلروسيز وممكن
+
+213
+00:16:05,160 --> 00:16:09,960
+تسبب سكتة دماغية و heart attack اللي هو الذبحة
+
+214
+00:16:09,960 --> 00:16:14,970
+القلبية أو الصدريةمعملنا هو عبارة عن isolation of
+
+215
+00:16:14,970 --> 00:16:18,010
+cholesterol from egg yolk حكينا مقدمة عن
+
+216
+00:16:18,010 --> 00:16:21,530
+الكوليسترول وعن انه عقل لكن ده بدنا ندخل في الجزء
+
+217
+00:16:21,530 --> 00:16:25,190
+العامل المعمل بيحكي انه بدنا نعزل الكوليسترول من
+
+218
+00:16:25,190 --> 00:16:28,870
+صفار البيض الجد ال egg كلها الهن egg اللي هي ال
+
+219
+00:16:28,870 --> 00:16:32,370
+egg كاملة بتتكون من egg white بياض البيض ومن ال
+
+220
+00:16:32,370 --> 00:16:37,030
+egg yolk اللي هو صفار البيض البيض بيحتوي على
+
+221
+00:16:37,030 --> 00:16:40,790
+مكوناته شوه عبارة عن إيش عبارة عن قلبه من أولبان
+
+222
+00:16:41,280 --> 00:16:45,900
+البيض عبارة عن قلبه من و لبن أما الصفار الصفار
+
+223
+00:16:45,900 --> 00:16:49,420
+البيض هو عبارة عن glycoprotein
+
+224
+00:16:54,080 --> 00:16:58,920
+Lipid حتى هو بيكون sorry نايت تاني الهين إيج الإيج
+
+225
+00:16:58,920 --> 00:17:02,460
+بيضة كاملة عبارة عن بياض البيض والصفار ال egg
+
+226
+00:17:02,460 --> 00:17:05,800
+white و ال egg yolk البياض ال egg white هو عبارة
+
+227
+00:17:05,800 --> 00:17:10,040
+عن ألبوم ان هو عبارة عن بروتين فقط البياض عبارة عن
+
+228
+00:17:10,040 --> 00:17:13,660
+بروتين الألبوم أما ال egg yolk اللي هو صفار البيض
+
+229
+00:17:13,660 --> 00:17:17,400
+هو عبارة عن lipid و glycoprotein عبارة عن بروتينات
+
+230
+00:17:17,400 --> 00:17:22,510
+سكرية مع دهوننجي لل إيجيوك اللي هو صفار البيت إيش
+
+231
+00:17:22,510 --> 00:17:28,450
+مكوناته؟ حكينا جلايكو بروتين و لبن عبارة عن 5% من
+
+232
+00:17:28,450 --> 00:17:34,750
+مكوناته هو كلسترول و 66% جليسرايد و 28% فوسفولبن
+
+233
+00:17:34,750 --> 00:17:39,730
+هي توزيع الدهون الموجودة في صفار البيت منها 5%
+
+234
+00:17:39,730 --> 00:17:42,710
+كلسترول اللي أنا بدي أخده أعزله بدي أسيب باقي
+
+235
+00:17:42,710 --> 00:17:48,610
+المكوناته بس بدي كلسترولالجيتل ايجيوك بيحكي ليه
+
+236
+00:17:48,610 --> 00:17:52,030
+انها غنية في الدهون صفار البيض هو غني بالدهون
+
+237
+00:17:52,030 --> 00:17:55,170
+بيحتوي على كمية عالية من الدهون من أشهر الدهون
+
+238
+00:17:55,170 --> 00:17:59,450
+اللي موجودة فيها هي ال glyceride كوليسترول و
+
+239
+00:17:59,450 --> 00:18:04,410
+phosphatidylcholine او اسمه التاني إليستين اذا
+
+240
+00:18:04,410 --> 00:18:07,690
+صفار البيض يحتوي على الليبد غني بالليبد أشهر
+
+241
+00:18:07,690 --> 00:18:12,920
+الليبد اللي موجودة فيه ال glycerideو الكلسترول
+
+242
+00:18:12,920 --> 00:18:16,320
+فوسفاتيدي الكولين اللي هو لستين احنا نعزل
+
+243
+00:18:16,320 --> 00:18:20,220
+الكلسترول الكلسترول اللي حكينا عنه اللي جتنا نحكي
+
+244
+00:18:20,220 --> 00:18:23,120
+عن فوسفاتيدي الكولين اشي بسيط عن فوسفاتيدي الكولين
+
+245
+00:18:23,120 --> 00:18:27,840
+او لستين نفس الاسم فوسفاتيدي الكولين او لستين هو
+
+246
+00:18:27,840 --> 00:18:32,240
+اشهر phospholipin موجود في الحيوان و النبات
+
+247
+00:18:32,240 --> 00:18:38,080
+فوسفاتيدي الكولين او لستينهو أشهر phospholipid
+
+248
+00:18:38,080 --> 00:18:45,000
+موجود عند الحيوان و النبات وظائف هو له تلت وظائف
+
+249
+00:18:45,000 --> 00:18:48,580
+هو ال key building block of membrane by layer هو
+
+250
+00:18:48,580 --> 00:18:52,760
+التركيبة الأساسية للطبقة الصناعية في ال membrane
+
+251
+00:18:52,760 --> 00:18:57,980
+يدخل في التركيبة الأساسية للممبرين by layer هي أول
+
+252
+00:18:57,980 --> 00:19:03,600
+وظيفة وظيفة تانية هو بشكل نسبة عالية في ال plasma
+
+253
+00:19:03,600 --> 00:19:08,860
+membraneالغلاف لـ Plasma high very high proportion
+
+254
+00:19:08,860 --> 00:19:12,900
+of the outer leaflet of the plasma membrane إذا هو
+
+255
+00:19:12,900 --> 00:19:16,300
+في ال membrane by layer وفيه ال plasma membrane
+
+256
+00:19:16,300 --> 00:19:21,020
+تالت وظيفة أن هو عبارة عن ال phospholipid الموجود
+
+257
+00:19:21,020 --> 00:19:25,450
+في ال lipoproteinإذا فاكرين الـ lipoprotein حكينا
+
+258
+00:19:25,450 --> 00:19:30,530
+إنه هي عبارة عن lipid مع protein مع phospholipid
+
+259
+00:19:30,530 --> 00:19:35,310
+ال phospholipid هاي هي عبارة عن فسفة تديلكولين أو
+
+260
+00:19:35,310 --> 00:19:39,750
+لستين إذا هو تلت وظائف له وظيفة الأولى بيدخل في
+
+261
+00:19:39,750 --> 00:19:43,250
+تركيب ال membrane by layer وظيفة التانية بيدخل في
+
+262
+00:19:43,250 --> 00:19:46,830
+تركيب البلازمة membrane وظيفة التالتة هو ال
+
+263
+00:19:46,830 --> 00:19:52,830
+phospholipid الموجود في ال lipoproteinأكتر فوسفو
+
+264
+00:19:52,830 --> 00:19:56,410
+لأكتر ليبو بروتين يحتوي على فوسفة D الكليين هو
+
+265
+00:19:56,410 --> 00:20:01,510
+الـHDL الليبو بروتين الحكينا فيه منها HDL و LDL و
+
+266
+00:20:01,510 --> 00:20:06,530
+VLDL و الكايلو مايكون تركيبتهم تحتوي على فوسفو
+
+267
+00:20:06,530 --> 00:20:10,290
+ليبد الفوسفو ليبد هذا عبارة عن فوسفة D الكليين مين
+
+268
+00:20:10,290 --> 00:20:13,990
+أكتر واحد يحتوي على فوسفة D الكليين في تركيبه هو
+
+269
+00:20:13,990 --> 00:20:15,270
+الـHDL
+
+270
+00:20:17,640 --> 00:20:21,180
+التجربة إن أنا بدي استخدم صفار البيض hard boiled
+
+271
+00:20:21,180 --> 00:20:25,060
+hen egg بدي أسلك بيضة بدي بيضة مسلوقة و بدي أخد
+
+272
+00:20:25,060 --> 00:20:30,000
+منها الصفار و بدي أستخدم أسيتون water bath flask
+
+273
+00:20:30,000 --> 00:20:38,540
+funnel filter paper و بيكهة ديش نشوف كدهأنا بدي
+
+274
+00:20:38,540 --> 00:20:41,260
+أعزل الكوليسترول من صفار البيض و أحسب ال
+
+275
+00:20:41,260 --> 00:20:47,100
+percentage ال
+
+276
+00:20:47,100 --> 00:20:50,100
+principle بناء على إيش أنا بدي أعزل الكوليسترول و
+
+277
+00:20:50,100 --> 00:20:54,720
+أسيب باقي المكوناتبيحكيلي بناء على ال solubility
+
+278
+00:20:54,720 --> 00:20:58,680
+in different organic solvents لجيت هم طب كل
+
+279
+00:20:58,680 --> 00:21:02,920
+المكونات لبن و كلهم هيدوبوا في نفس الأشياء كيف بدي
+
+280
+00:21:02,920 --> 00:21:05,860
+أخد الكوليسترول و أسيب كل الباقي اللبن بناء على ال
+
+281
+00:21:05,860 --> 00:21:09,760
+solubility؟ بيحكيلي إنه وجدوا أن الكوليسترول هو
+
+282
+00:21:09,760 --> 00:21:13,780
+الوحيد اللي بيكون soluble in أسيتون لما نستخدم
+
+283
+00:21:13,780 --> 00:21:19,450
+أسيتونأحطه على صفار البيض بس الكوليسترول بيدوب
+
+284
+00:21:19,450 --> 00:21:24,190
+والباقي كله بيترسب كل المكونات بتترسب معادا
+
+285
+00:21:24,190 --> 00:21:29,330
+الكوليسترول بيدوب فالمبدأ أني أستخدم أسيتون يعمل
+
+286
+00:21:29,330 --> 00:21:34,610
+على إذابة الكوليسترول فقط وباقي المكونات بتكون
+
+287
+00:21:34,610 --> 00:21:40,790
+insoluble بتكون مترسمة إيش هو الأسيتون؟الاسيتون
+
+288
+00:21:40,790 --> 00:21:45,470
+اسمه الاخر بروبانون بروبانون اسمه الاخر بروبانون
+
+289
+00:21:45,470 --> 00:21:50,650
+هو عبارة عن organ ال compound مركب عضوي له صيغة
+
+290
+00:21:50,650 --> 00:21:56,030
+CH3 لكل اتنين CO هو خصائصه colorless لا لون له
+
+291
+00:21:56,030 --> 00:22:02,490
+volatile متطاير flammable قابل الاشتعال وهو أبسط
+
+292
+00:22:02,490 --> 00:22:07,440
+كيتون هو كيتون يعني إيش صيغة الكيتون؟انه C double
+
+293
+00:22:07,440 --> 00:22:11,660
+bond O وR من جهها وR من جهها هو أبسط كيتون لأن
+
+294
+00:22:11,660 --> 00:22:14,600
+الـR من جهها هي عبارة عن CH3 والـR من الجهة
+
+295
+00:22:14,600 --> 00:22:19,720
+التانية هي CH3 برضه تمام، إذا ال principle بناء
+
+296
+00:22:19,720 --> 00:22:23,460
+على استخدام الاسيتون اللي بيدوب، بيدوّب
+
+297
+00:22:23,460 --> 00:22:30,280
+الكوليسترول وباقى المكونات بتترسم إيش الخطوات؟ بدي
+
+298
+00:22:30,280 --> 00:22:34,890
+أجيب بيضة مسلوبة بدي أشيل عنها البيعهجيب بيضة
+
+299
+00:22:34,890 --> 00:22:37,830
+مسلوجة انا مابديش البيض بدي اتخلص منه و بدي اخد
+
+300
+00:22:37,830 --> 00:22:42,970
+السفارة بدي اخد نص السفارة نص ال egg نص السفارة ال
+
+301
+00:22:42,970 --> 00:22:48,330
+egg و اوزنها بدي اخد نص السفارة اوزنها بعدين بدي
+
+302
+00:22:48,330 --> 00:22:52,150
+اخد احطها ب بيكر و اطحنها اعمل mashed egg mashed
+
+303
+00:22:52,150 --> 00:22:57,130
+يعني اطحنها احط عليها ال acetone خمسة و عشرين مل
+
+304
+00:22:57,130 --> 00:23:01,610
+من ال acetone و بعمل mix لمدة خمس دقايق اذا انا
+
+305
+00:23:01,610 --> 00:23:05,810
+اخدت نص السفارة البيضةوزنتها عشان هتهمني هذه
+
+306
+00:23:05,810 --> 00:23:11,110
+الوزنة في ال percentage في الحسابات بعدين بحطها ب
+
+307
+00:23:11,110 --> 00:23:14,510
+بيكر هذا النص الصفر البيضا بحطها ب بيكر و بطحنها
+
+308
+00:23:14,510 --> 00:23:19,320
+هحط عليها خمسة و عشرين مل من الاسيتونو بعدين اضنني
+
+309
+00:23:19,320 --> 00:23:24,060
+احرك لمدة خمس دقايق بعد خمس دقايق يفترض ال acetone
+
+310
+00:23:24,060 --> 00:23:29,320
+الشمال بيترسب كل المكونات في الراسب و فوق السائل
+
+311
+00:23:29,320 --> 00:23:33,660
+يحتوي على acetone مع كلسترول انا بدي السائل بديش
+
+312
+00:23:33,660 --> 00:23:37,480
+الطبق اللي تحت بدي السائل اللي فوق اللي هو عبارة
+
+313
+00:23:37,480 --> 00:23:41,440
+عن acetone وكلسترول لان كلسترولالوحيد اللي بيدور
+
+314
+00:23:41,440 --> 00:23:45,060
+في الـ Acetone إيش بدي أعمل؟ بدي أسحب .. أحاول
+
+315
+00:23:45,060 --> 00:23:48,980
+أسحب السائل هذا اللي فوق أسحب الطبقة اللي فوق بـ
+
+316
+00:23:48,980 --> 00:23:52,740
+dropper أو بأي شيء بدي أسحب الطبقة اللي فوق السائل
+
+317
+00:23:52,740 --> 00:23:57,660
+و أحطه في بيكر تانيوأسكر عليها عشان مايتطيرش
+
+318
+00:23:57,660 --> 00:24:02,940
+الاسيتون أرجع تاني على الترسب اللي دال عندي في
+
+319
+00:24:02,940 --> 00:24:06,920
+البيكر ال recipe اللي تحت باضيف عليها كمان 25 مل
+
+320
+00:24:06,920 --> 00:24:11,530
+من الاسيتون وأعمل mix أكرر الخطوة اللي جابانأنا
+
+321
+00:24:11,530 --> 00:24:16,310
+جبت نص صفر البيض و طحنته و حطيت عليه 25 مل من
+
+322
+00:24:16,310 --> 00:24:20,650
+الأسيتون و عملت mix هيكون فوق في السائل في عندي
+
+323
+00:24:20,650 --> 00:24:24,470
+أسيتون مع كوليسترول و عندي راسب تحت بدي أخد السائل
+
+324
+00:24:24,470 --> 00:24:28,340
+هذا اللي فوق أنجله على بيكر تانيو بعدين الطبقة
+
+325
+00:24:28,340 --> 00:24:31,520
+اللى تحتها دى عشان اتأكد ان اخد كل الكوليسترول
+
+326
+00:24:31,520 --> 00:24:37,500
+بضيف كمان 25 مل أسيتون و اضلنى أحرق و بعدين اخد
+
+327
+00:24:37,500 --> 00:24:39,760
+السائل اللى فوق اللى هو عبارة عن أسيتون و
+
+328
+00:24:39,760 --> 00:24:43,440
+كوليسترول اضيفه على الجزء الأول على ال beaker اللى
+
+329
+00:24:43,440 --> 00:24:48,360
+نجلته من الجزء الأول بيصير عندى هيك 50 مل 25 مرة
+
+330
+00:24:48,360 --> 00:24:51,700
+الأولى و 25 مرة التانية عبارة عن أسيتون مع
+
+331
+00:24:51,700 --> 00:24:57,530
+كوليسترولهلجيت بديش انا مين؟ عشان اتأكد ان انا
+
+332
+00:24:57,530 --> 00:25:02,410
+مااخدتش راسب بده اعمل فلترة بعد ما نجلت الاسيتون و
+
+333
+00:25:02,410 --> 00:25:05,670
+الكوليسترول بده اعمل فلترة بده اجيب فلوسك و اجيب
+
+334
+00:25:05,670 --> 00:25:11,100
+فيها funnel محجن و filter بيماوأديره في هذا ال
+
+335
+00:25:11,100 --> 00:25:14,260
+filter paper على ال filter paper خلية ي filter
+
+336
+00:25:14,260 --> 00:25:18,840
+هينزل عند السائل ومين هو السائل؟ السائل عبارة عن
+
+337
+00:25:18,840 --> 00:25:22,140
+أسيتون مع كوليسترون أما الراسب اللي بيضال في ال
+
+338
+00:25:22,140 --> 00:25:26,220
+filter paper هذا بدّيش هيانا بدي السائل اللي هو
+
+339
+00:25:26,220 --> 00:25:30,140
+يحتوي على كوليسترول باخد السائل هاد من ال flask
+
+340
+00:25:30,140 --> 00:25:33,760
+بعد ما يخلص فلترة بدي أرمي الورقة و أخد السائل
+
+341
+00:25:33,760 --> 00:25:37,940
+السائل هاد عبارة عن أسيتون مع كوليسترول بديش
+
+342
+00:25:37,940 --> 00:25:41,760
+الأسيتون بدي بس كوليسترول الأسيتون حكينا من خصائصه
+
+343
+00:25:41,760 --> 00:25:46,170
+أنه volatile إنه متطاير تمام؟فاش بدي أسوي بدي أخده
+
+344
+00:25:46,170 --> 00:25:50,470
+في water bath في ميه سخنه بدي أحط ال baker الميه
+
+345
+00:25:50,470 --> 00:25:54,770
+السخنه بتساعد على أنه يطير ال acetone بسرعة بيطير
+
+346
+00:25:54,770 --> 00:25:59,450
+ال acetone و بيضل عندي بس كلسترول باخده و بحطه على
+
+347
+00:25:59,450 --> 00:26:03,630
+تلج على ice عشان يعمل heat shock صدمة حرارية
+
+348
+00:26:03,630 --> 00:26:08,010
+للكلسترول أنه يتجمع مع بعض بيتجمع عندي على شكل
+
+349
+00:26:08,010 --> 00:26:14,390
+crystal الكوليسترول بيتجمع على شكل crystalهذه
+
+350
+00:26:14,390 --> 00:26:18,910
+الكريستال هي عبارة عن الكوليسترول باخدها و بوزنها
+
+351
+00:26:18,910 --> 00:26:22,990
+يعني البيكر بكون وزنها من الأول وزن وزنته وعارف
+
+352
+00:26:22,990 --> 00:26:27,530
+جداش وزنته عشان أنقص بعد هيك الوزنة لإن لما اوزن
+
+353
+00:26:27,530 --> 00:26:30,690
+الكوليسترول في البيكر هيوزنني اتنين مع بعض
+
+354
+00:26:30,690 --> 00:26:35,730
+كوليسترول مع البيكر فانقص الوزنة زي ما عملنا أيام
+
+355
+00:26:35,730 --> 00:26:39,450
+ال كازين لما وزننا الورجة و بعدين وزننا الورجة مع
+
+356
+00:26:39,450 --> 00:26:44,360
+الكازينتمام و باخد وزنة الكوليسترول و بدي أحسبله
+
+357
+00:26:44,360 --> 00:26:48,200
+ال percentage ال percentage للكوليسترول إيش بتساوي
+
+358
+00:26:48,200 --> 00:26:51,440
+weight of الكوليسترول وزنة الكوليسترول اللي طلعت
+
+359
+00:26:51,440 --> 00:26:55,480
+معاكي في الآخر اللي وزنتيها على وزن نص البيضة اللي
+
+360
+00:26:55,480 --> 00:27:00,180
+وزنناها في الأول لما حكينا أنه نص صفار اللي أخدناه
+
+361
+00:27:00,180 --> 00:27:06,000
+ضرب 100% و بحسب وزنة الكوليسترول اللي موجودة في
+
+362
+00:27:06,000 --> 00:27:14,050
+الصفار البيضو هيك بيكون خلصنا هذا المعمل و يعطيكم
+
+363
+00:27:14,050 --> 00:27:14,490
+العافية
+
diff --git a/PL9fwy3NUQKwZ1Q7zOg1ObgufPMjRI3WY1/EDL86sMHiEI.srt b/PL9fwy3NUQKwZ1Q7zOg1ObgufPMjRI3WY1/EDL86sMHiEI.srt
new file mode 100644
index 0000000000000000000000000000000000000000..7764f919c64a3e7ba67d250809491107ff54572a
--- /dev/null
+++ b/PL9fwy3NUQKwZ1Q7zOg1ObgufPMjRI3WY1/EDL86sMHiEI.srt
@@ -0,0 +1,2329 @@
+1
+00:00:00,490 --> 00:00:04,790
+اليوم إن شاء الله بنحكي في معمل جديد اسمه ال
+
+2
+00:00:04,790 --> 00:00:10,170
+chromatography الـ chromatography هي تقنية لفصل
+
+3
+00:00:10,170 --> 00:00:15,630
+الخليط من المواد إلى مكوناتها عندي خليط من المواد 
+
+4
+00:00:15,630 --> 00:00:22,170
+من المواد لأفصل كل مكون على حدة في البداية
+
+5
+00:00:22,170 --> 00:00:27,150
+نحكي مقدمة عن هذه الطريقة و كيف تم اكتشافها في
+
+6
+00:00:27,150 --> 00:00:32,530
+عندنا عالم اسمه Michael Tswett هاد عالم روسي استخدم
+
+7
+00:00:32,530 --> 00:00:37,070
+هذه التقنية لفصل أصباغ النباتات وورقة النباتات
+
+8
+00:00:37,070 --> 00:00:41,670
+تحتوي على عدة أنواع من الأصباغ قام في فصل هذه
+
+9
+00:00:41,670 --> 00:00:47,130
+الأصباغ بطريقة أطلق عليها اسم chromatography حكى
+
+10
+00:00:47,130 --> 00:00:50,030
+أن chromatography تعني written color
+
+11
+00:00:50,030 --> 00:00:54,730
+chromatography تعني written color معناها الكتابة
+
+12
+00:00:54,730 --> 00:01:01,040
+بالألوان لأن هو استخدم هذه الطريقة لفصل أصباغ
+
+13
+00:01:01,040 --> 00:01:06,320
+النباتات فأطلق عليها إن هي الكتابة بالألوان إنها
+
+14
+00:01:06,320 --> 00:01:11,520
+تستخدم للمواد الملونة لأن هو فصل مواد ملونة فصل 
+
+15
+00:01:11,520 --> 00:01:17,730
+أصباغ يحكي لإن معظم المواد المحيطة فينا هي حوالينا
+
+16
+00:01:17,730 --> 00:01:22,030
+هي عبارة عن خليط من مادتين أو أكثر most materials
+
+17
+00:01:22,030 --> 00:01:24,350
+in our surroundings are a mixture of two or more
+
+18
+00:01:24,350 --> 00:01:28,150
+compounds إن معظم المكونات في الهواء الجوي أو
+
+19
+00:01:28,150 --> 00:01:33,450
+حوالينا في أي مكان هي عبارة عن خليط هذا الخليط إما
+
+20
+00:01:33,450 --> 00:01:38,870
+بيكون homogeneous أو heterogeneous الخليط إما بيكون
+
+21
+00:01:38,870 --> 00:01:43,570
+خليط متجانس homogenous أو خليط غير متجانس
+
+22
+00:01:43,570 --> 00:01:47,990
+heterogeneous إيش يعني homogenous are uniform in
+
+23
+00:01:47,990 --> 00:01:52,110
+composition؟ homogenous معناه خليط متجانس إنه
+
+24
+00:01:52,110 --> 00:01:58,470
+عبارة عن عدة مكونات ولكن يظهر كمادة واحدة زي مثلا
+
+25
+00:01:58,470 --> 00:02:01,630
+الهواء الجوي اللي حوالنا يحتوي على عدة أنواع من
+
+26
+00:02:01,630 --> 00:02:06,270
+الغازات لكن هل مبين كل غاز؟ لأ مبين إن هو مكون
+
+27
+00:02:06,270 --> 00:02:11,150
+واحد heterogeneous إن الـ mixture are not uniform
+
+28
+00:02:11,150 --> 00:02:15,590
+إنّه عندنا خليط من عدة مكونات لكن مبين إن هذا يحتوي
+
+29
+00:02:15,590 --> 00:02:20,900
+على أكثر من مادة زي مين؟ زي الزيت مع الميه تخلط
+
+30
+00:02:20,900 --> 00:02:24,680
+الزيت مع الميه مبين وبيفصلوا لطبقتين فمبين ان هو
+
+31
+00:02:24,680 --> 00:02:28,400
+عبارة عن أكثر من مادة عبارة عن مادتين واضحين مش 
+
+32
+00:02:28,400 --> 00:02:32,460
+متجانسين هي مثال مكتوب أن الـ air هو عبارة عن
+
+33
+00:02:32,460 --> 00:02:35,580
+homogeneous mixture أما الـ oil في الـ water لما
+
+34
+00:02:35,580 --> 00:02:39,580
+يكون زيت مع مي فهو heterogeneous غير متجانس بيحكي
+
+35
+00:02:39,580 --> 00:02:42,920
+لي سواء الخليط كان homogeneous أو heterogeneous
+
+36
+00:02:42,920 --> 00:02:47,560
+نقدر نفصله نعمل separated by physical method بطرق
+
+37
+00:02:47,560 --> 00:02:51,760
+فيزيائية عدة، إذا سواء الخليط كان عندي متجانس أو
+
+38
+00:02:51,760 --> 00:02:57,000
+غير متجانس بقدر أفصل مكوناته باستخدام عدة طرق في
+
+39
+00:02:57,000 --> 00:03:01,920
+طرق فيزيائية كيف بدأ يختار الطريقة؟ احنا عندنا عدة
+
+40
+00:03:01,920 --> 00:03:05,120
+طرق للـ separation كيف بدأ يختار الطريقة المناسبة
+
+41
+00:03:05,120 --> 00:03:08,580
+لفصل الخليط بناء على الـ type of mixture على نوع
+
+42
+00:03:08,580 --> 00:03:11,160
+الخليط على الـ difference in the chemical
+
+43
+00:03:11,160 --> 00:03:15,760
+properties على الخصائص الكيميائية للمكونات مثلا أنا
+
+44
+00:03:15,760 --> 00:03:21,100
+هختار طريقة الفصل بناء على نوع الخليط أو بناء على
+
+45
+00:03:21,100 --> 00:03:25,340
+الخصائص الكيميائية يعني مثلا من طرق الفصل مثلا
+
+46
+00:03:25,340 --> 00:03:28,280
+عندنا الـ centrifugation الـ centrifugation يمكن
+
+47
+00:03:28,280 --> 00:03:33,110
+أخذناه بالمقدمة أن استخدام جهاز centrifuge بيفصل
+
+48
+00:03:33,110 --> 00:03:38,170
+بناءً على الكثافة الدم مثلا نفصله باستخدام هذا
+
+49
+00:03:38,170 --> 00:03:42,310
+الجهاز باستخدام الـ centrifuge مثلا زي ما استخدمنا
+
+50
+00:03:42,310 --> 00:03:46,090
+isolation زي ما عزلنا الكازين من الحليب هي هذه
+
+51
+00:03:46,090 --> 00:03:49,310
+طريقة فصل أن أنا بس أخدت الكازين من كل مكونات
+
+52
+00:03:49,310 --> 00:03:54,150
+الحليب كانت الطريقة اسمها isolation مثلا عملية
+
+53
+00:03:54,150 --> 00:03:58,230
+precipitation أني أرسب مكون وأسيب الباقي وفي عندنا
+
+54
+00:03:58,230 --> 00:04:02,110
+تقنية chromatography وفي عندنا مثلا الـ evaporation
+
+55
+00:04:02,110 --> 00:04:06,530
+التبخر أعمل تبخر زي مثلا مي مع ملح ببخر المي وبخلي
+
+56
+00:04:06,530 --> 00:04:11,470
+الملح مثلا الـ extraction استخلاص أعمل استخلاص
+
+57
+00:04:11,470 --> 00:04:15,570
+للمادة كل هذول طرق للفصل اليوم احنا الطريقة اللي
+
+58
+00:04:15,570 --> 00:04:20,370
+بدنا ناخدها هي الـ chromatography بحكي للـ
+
+59
+00:04:20,370 --> 00:04:23,930
+chromatography مستخدمة بشكل واسع لفصل الـ mixture
+
+60
+00:04:23,930 --> 00:04:29,050
+of compounds into individual components لفصل الخليط
+
+61
+00:04:29,050 --> 00:04:33,890
+إلى مكوناته إنّه بيخلي كل مادة لحال هذه التقنية
+
+62
+00:04:33,890 --> 00:04:38,630
+مستخدمة بشكل واسع لفصل الخليط إيش معنى 
+
+63
+00:04:38,630 --> 00:04:41,850
+chromatography؟ chromatography لأن في البداية
+
+64
+00:04:41,850 --> 00:04:45,750
+استخدموها لفصل الألوان، لأصباغ النباتات، فأطلقوا
+
+65
+00:04:45,750 --> 00:04:50,350
+عليها أنها مستخدمة لـ separation of color، لفصل
+
+66
+00:04:50,350 --> 00:04:54,130
+الألوان، ولكن في الوقت الحالي today الـ
+
+67
+00:04:54,130 --> 00:04:57,680
+chromatography تعني فصل الـ color والـ colorless
+
+68
+00:04:57,680 --> 00:05:00,940
+substance إنّها تستخدم للمواد الملونة وغير
+
+69
+00:05:00,940 --> 00:05:05,180
+الملونة، فإنّ هي قديما أول ما اكتشفت التقنية كانت
+
+70
+00:05:05,180 --> 00:05:09,240
+فقط بيعتقدوا إنّها للمواد الملونة، ولكن في اليوم
+
+71
+00:05:09,240 --> 00:05:14,420
+الحاضر هي المواد الملونة وغير الملونة الـ
+
+72
+00:05:14,420 --> 00:05:17,360
+chromatography هي طريقة فيزيائية physical method
+
+73
+00:05:17,360 --> 00:05:21,040
+for separation of mixture to its components
+
+74
+00:05:21,040 --> 00:05:26,820
+لمكوناته بناء على two phases باستخدام two phases طوران
+
+75
+00:05:26,820 --> 00:05:31,920
+حاجة بنسميها stationary phase وحاجة بنسميها الـ
+
+76
+00:05:31,920 --> 00:05:37,620
+mobile phase إذن هي تقنية فيزيائية أو طريقة
+
+77
+00:05:37,620 --> 00:05:43,220
+فيزيائية لفصل الخليط إلى مكوناته باستخدام two phases
+
+78
+00:05:43,220 --> 00:05:48,260
+الـ mobile phase والـ stationary phase إيش هو الـ
+
+79
+00:05:48,260 --> 00:05:51,380
+stationary phase الـ stationary phase هو عبارة عن
+
+80
+00:05:51,380 --> 00:05:57,760
+الجزء الصلب بيكون solid مدعم بـ glass بجزاز أو
+
+81
+00:05:57,760 --> 00:06:02,400
+بتكون عبارة عن paper عن ورقة إذا الـ stationary
+
+82
+00:06:02,400 --> 00:06:08,000
+phase هو الطور الساكن هو الطور الساكن بيكون عبارة
+
+83
+00:06:08,000 --> 00:06:12,960
+عن طبعا ورقة ممكن تكون صلبة هي ورقة صلبة تكون مدعمة
+
+84
+00:06:12,960 --> 00:06:17,540
+من الخلف بزجاج أو ألمنيوم عشان تحميها من التكسر
+
+85
+00:06:17,540 --> 00:06:22,320
+وممكن تكون عبارة عن porous material هي مادة مثقبة الـ stationary phase هي مادة
+
+86
+00:06:22,320 --> 00:06:27,200
+مثقبة porous material ليش؟ عشان تسمح بدخول السائل The
+
+87
+00:06:27,200 --> 00:06:33,220
+sample is carried by a particular solvent called
+
+88
+00:06:33,220 --> 00:06:36,400
+mobile phase كنت أنا كيف تقنيتها دي بيكون في عندي
+
+89
+00:06:36,400 --> 00:06:40,080
+ورقة اسمها stationary phase هذا الطور الساكن هاي
+
+90
+00:06:40,080 --> 00:06:44,380
+بحط عليها العينات الخليط اللي أنا بدي أفصله بحط
+
+91
+00:06:44,380 --> 00:06:48,240
+نقطة من الخليط اللي أنا بأفصله على الـ stationary
+
+92
+00:06:48,240 --> 00:06:50,860
+phase على الطور الساكن في عندي حاجة اسمها الـ
+
+93
+00:06:50,860 --> 00:06:54,060
+mobile phase الـ mobile phase هو الطور المتحرك
+
+94
+00:06:54,060 --> 00:06:57,680
+بيكون عبارة عن غاز أو liquid أو سائل وجدت الورقة
+
+95
+00:07:03,070 --> 00:07:05,750
+الـ stationary phase اللي أنا حطيت عليها الخليط
+
+96
+00:07:05,750 --> 00:07:11,900
+بقى حطها بغمرها في الـ mobile phase بغمر الورقة في
+
+97
+00:07:11,900 --> 00:07:14,840
+الـ mobile phase بيبدأ الـ mobile phase اللي هو
+
+98
+00:07:14,840 --> 00:07:18,940
+عبارة عن سائل أنّه يمر عبر هذه الورقة زي مثلا الـ
+
+99
+00:07:18,940 --> 00:07:22,460
+tissue paper لما تحطيها بمي بتتبلل بالمي بيمر المي
+
+100
+00:07:22,460 --> 00:07:25,860
+عبر الـ tissue paper وهان هيك الـ stationary phase
+
+101
+00:07:25,860 --> 00:07:28,420
+هي الورقة بيكون عليها الخليط أنا حاطة نقطة من
+
+102
+00:07:28,420 --> 00:07:31,420
+الخليط عليها وبغمرها في الـ mobile phase اللي هو
+
+103
+00:07:31,420 --> 00:07:35,870
+عبارة عن سائل بيمر السائل في هذه الورقة طول ما هو
+
+104
+00:07:35,870 --> 00:07:40,490
+بيمشي السائل بيفصل معاه الخليط لمكوناته على هيئة
+
+105
+00:07:40,490 --> 00:07:44,870
+نقاط كل .. كل مكون من هذه المكونات بيعطينا نقطة
+
+106
+00:07:44,870 --> 00:07:48,910
+في مكان معين فالـ Mobile phase هو عبارة عن الـ
+
+107
+00:07:48,910 --> 00:07:53,030
+solvent اسمه ممكن نحكي عنه الـ solvent أو الـ eluent
+
+108
+00:07:53,030 --> 00:07:58,170
+هو بيكون liquid أو gas الـ mobile phase بنبقى
+
+109
+00:07:58,170 --> 00:08:02,770
+الستاشنري stationary phase الستاشنري فيس هو جزء
+
+110
+00:08:02,770 --> 00:08:07,450
+الصلب بيكون عبارة عن ورقة ممكن وهي صلب بتكون
+
+111
+00:08:07,450 --> 00:08:11,830
+مدعمة بجلاس أو ألمنيوم الـ mobile phase عبارة عن
+
+112
+00:08:11,830 --> 00:08:16,030
+solvent اللي you want liquid أو غاز بيكون سائل أو
+
+113
+00:08:16,030 --> 00:08:21,730
+غاز بغمر الـ stationary phase في الـ mobile phase
+
+114
+00:08:21,730 --> 00:08:24,850
+بيحكي لي بعدين الـ mobile phase بيمر عبر الـ
+
+115
+00:08:24,850 --> 00:08:27,810
+stationary phase هيبدأ يمر داخل الـ stationary
+
+116
+00:08:27,810 --> 00:08:31,910
+phase الورقة أنا لما أغمرها في السائل بيمشي السائل
+
+117
+00:08:31,910 --> 00:08:35,570
+فيها للأعلى فبيمر الـ mobile phase عبر الـ
+
+118
+00:08:35,570 --> 00:08:39,630
+stationary phase طب في عندنا عدة مصطلحات، في عندنا
+
+119
+00:08:39,630 --> 00:08:43,830
+حاجة بنسميها affinity affinity هي المحبة معناها أو
+
+120
+00:08:43,830 --> 00:08:48,090
+التجاذب affinity تعني المحبة أو التجاذب هي
+
+121
+00:08:48,090 --> 00:08:51,030
+attraction or force between .. natural attraction
+
+122
+00:08:51,030 --> 00:08:54,330
+or force between two مواد هو التجاذب الطبيعي
+
+123
+00:08:54,330 --> 00:08:58,950
+للمواد مع بعضها البعض تجاذب طبيعي للمواد مع
+
+124
+00:08:58,950 --> 00:09:04,030
+بعضها البعض مثلًا المي والسكر هاد بولر وهاد بولر
+
+125
+00:09:04,030 --> 00:09:08,270
+بيدوبوا ببعض يعني هما محببين لبعض فهما نفس الخصائص
+
+126
+00:09:08,270 --> 00:09:12,410
+فبيدوبوا ببعض لذن الـ affinity هي التجاذب الطبيعي
+
+127
+00:09:12,410 --> 00:09:17,590
+للمواد مع بعضها البعض adsorption في إن adsorption
+
+128
+00:09:17,590 --> 00:09:22,090
+و absorption adsorption هي adhesion or stickness
+
+129
+00:09:22,090 --> 00:09:24,910
+of substance to the surface of another substance
+
+130
+00:09:24,910 --> 00:09:32,270
+والتصاق مادة على سطح مادة أخرى التصاق مادة على سطح
+
+131
+00:09:32,270 --> 00:09:36,430
+مادة أخرى التصاق المادة بس على السطح بدون اختراق
+
+132
+00:09:36,430 --> 00:09:40,640
+التركيب الداخلي للمادة هاي الـ absorption absorption
+
+133
+00:09:40,640 --> 00:09:47,000
+التصاق مادة على سطح مادة أخرى absorption معناها أن
+
+134
+00:09:47,000 --> 00:09:51,580
+المادة تخترق التركيب الداخلي للمادة الأخرى مش
+
+135
+00:09:51,580 --> 00:09:56,880
+التصاق فقط على السطح وإنما اختراق التركيب الداخلي
+
+136
+00:09:56,880 --> 00:10:02,020
+للمادة فيها نوعين رابط يوتيوب هاي بوضح الفرق بين
+
+137
+00:10:02,020 --> 00:10:06,660
+الـ absorption والـ absorption إذا حبين تطلعوا عليه
+
+138
+00:10:08,390 --> 00:10:12,550
+هنا توضيح لتقنية الـ chromatography نيجي أول شي على
+
+139
+00:10:12,550 --> 00:10:17,150
+اليسار في عندنا هنا الورقة البيضاء هذه الورقة هي الـ
+
+140
+00:10:17,150 --> 00:10:21,310
+stationary phase لذن الورقة هي الطور الساكن اللي
+
+141
+00:10:21,310 --> 00:10:24,590
+حكينا عنها إنّها stationary phase بتكون solid بتكون
+
+142
+00:10:24,590 --> 00:10:29,650
+مدعمة هلقيت؟ الخليط أنا وإنه بدي أحطه .. المكونات ..
+
+143
+00:10:29,650 --> 00:10:32,690
+عندي خليط عندي كيتوب مليانة فيها خليط من المكونات
+
+144
+00:10:32,690 --> 00:10:36,210
+وبدي أفصلها بأخد منها نقطة بحطها على هذه الورقة
+
+145
+00:10:36,210 --> 00:10:40,540
+على الـ stationary phase في موقع معين تمام؟ وبعدين
+
+146
+00:10:40,540 --> 00:10:44,460
+بأخد هذه الورقة اللي صار عليها الخليط بغمرها في
+
+147
+00:10:44,460 --> 00:10:48,560
+داخل الـ mobile phase الـ mobile phase هو الجزء الأزرق
+
+148
+00:10:48,560 --> 00:10:51,160
+اللي تحت احنا هلقيت بالصورة اللي على الشمال
+
+149
+00:10:51,160 --> 00:10:54,440
+الورقة البيضاء هي الـ stationary phase الطور الساكن
+
+150
+00:10:54,440 --> 00:10:58,100
+حطيت عليها نقطة من هذا الخليط والخليط كان عبارة
+
+151
+00:10:58,100 --> 00:11:02,820
+عن عدة مكونات هي تحت ملونة في منها أصفر وأحمر و
+
+152
+00:11:02,820 --> 00:11:05,440
+أزرق إنّها تحتوي على عدة مكونات
+
+153
+00:11:13,230 --> 00:11:17,560
+الجزء الأزرق اللي تحت هو عبارة عن liquid عن سائل لا
+
+154
+00:11:17,560 --> 00:11:21,500
+جاتش بدي يسير بدي أُمر هذا السائل عبر الـ stationary
+
+155
+00:11:21,500 --> 00:11:25,100
+phase بيسير يمشي فيه عبر خاصية الـ capillary
+
+156
+00:11:25,100 --> 00:11:28,600
+action الخاصية الشعرية لأن الـ stationary phase
+
+157
+00:11:28,600 --> 00:11:33,120
+حكينا إنّها مادة مثقبة porous material فبيمر السائل
+
+158
+00:11:33,120 --> 00:11:37,180
+الـ mobile phase عبرها وبيصعد للأعلى وبيظل يمشي طيب
+
+159
+00:11:37,180 --> 00:11:40,100
+كيف ده تفصل المواد طول ما هو بيمشي بيفصل الخليط
+
+160
+00:11:40,100 --> 00:11:44,200
+ لعدد مكوناته كل واحدة بتوقف في مكان بناء على إيش؟
+
+161
+00:11:44,200 --> 00:11:50,100
+بناء على الـ polarity وبناء على الـ solubility كيف؟
+
+162
+00:11:50,100 --> 00:11:52,440
+إن الورقة اللي أنا بستخدمها الـ stationary phase
+
+163
+00:11:52,440 --> 00:11:56,420
+بيكون إلها خصائص يعني ممكن تكون polar أو ممكن تكون
+
+164
+00:11:56,420 --> 00:11:59,800
+non-polar على اعتبار مثلا الـ stationary phase هذه
+
+165
+00:11:59,800 --> 00:12:03,120
+اللي استخدمتها هي polar والـ mobile phase برضه
+
+166
+00:12:03,120 --> 00:12:06,460
+بيكون إله خصائص إذا الـ stationary phase كان polar
+
+167
+00:12:06,460 --> 00:12:10,560
+إذا الـ mobile phase non-polar تمام أنا بدي أعتبر الـ
+
+168
+00:12:10,560 --> 00:12:13,840
+stationary phase هنا إن هو polar والـ mobile phase
+
+169
+00:12:13,840 --> 00:12:17,420
+non-polar اجت من اللي بيمشي الـ mobile phase هو
+
+170
+00:12:17,420 --> 00:12:21,260
+اللي بيصعد الـ stationary phase سابتة مدام الـ
+
+171
+00:12:21,260 --> 00:12:25,500
+mobile phase اللي بيصعد هو non-polar فالمواد اللي
+
+172
+00:12:25,500 --> 00:12:29,320
+في الخليط إذا كانت non-polar هتمشي مع الـ mobile
+
+173
+00:12:29,320 --> 00:12:34,740
+phase لفوق تمشي معاه إذا كانت polar هتقف لتحت عند
+
+174
+00:12:34,740 --> 00:12:37,700
+الـ stationary phase هتظلها سابتة تحت ومش هتمشي
+
+175
+00:12:38,360 --> 00:12:41,680
+لأنّها بتحب الـ stationary phase هي خصائصها polar زي
+
+176
+00:12:41,680 --> 00:12:45,300
+الـ stationary phase إذا هتوجه فتحت وتضل ملزقة
+
+177
+00:12:45,300 --> 00:12:48,460
+فيه في الـ stationary phase مش هتمشي مع الـ mobile اللي
+
+178
+00:12:48,460 --> 00:12:52,500
+تكون خصائصها زي الـ mobile phase مثلا non-polar زيه
+
+179
+00:12:52,50
+
+201
+00:14:18,260 --> 00:14:23,930
+عدة مكونات صار كل مكون لحاله عملت له quantify كمي
+
+202
+00:14:23,930 --> 00:14:28,010
+أنه أنا عرفت هذا الخليط مكون من كم من مادة يحتوي
+
+203
+00:14:28,010 --> 00:14:32,130
+على كم من مادة يعني هو فصل لعدة مواد كل واحد في 
+
+204
+00:14:32,130 --> 00:14:36,910
+منطقة مثلا الأصفر هي مادة أحمر مادتين ودوائر
+
+205
+00:14:36,910 --> 00:14:40,930
+السودة هي ثلاث مواد والمستطيلات هي أربع مواد إذا
+
+206
+00:14:40,930 --> 00:14:46,820
+هذا الخليط يتكون من أربع مواد هنا التفصيل للي
+
+207
+00:14:46,820 --> 00:14:51,060
+توحكناه كله تقنية الـ separation is based on the fact
+
+208
+00:14:51,060 --> 00:14:54,280
+that different solid absorbed different substances to
+
+209
+00:14:54,280 --> 00:14:57,720
+different extreme depending on polarity أنه عندنا
+
+210
+00:14:57,720 --> 00:15:01,620
+مادة بتكون بورص اللي هي عبارة عن stationary phase
+
+211
+00:15:02,220 --> 00:15:07,360
+بتكون بورص material مادة مثقبة أنا هحط عليها القليل
+
+212
+00:15:07,360 --> 00:15:11,360
+اللي أنا بدي أفصله وهيتم الفصل أو أنها تلتصق في
+
+213
+00:15:11,360 --> 00:15:14,280
+هذه الـ stationary phase المكون إذا لتصق في ال
+
+214
+00:15:14,280 --> 00:15:17,320
+stationary phase أو أنه يمشي مع الـ mobile phase
+
+215
+00:15:17,320 --> 00:15:21,440
+وما يلزجش بناء على إيش؟ بناء على الـ polarity على 
+
+216
+00:15:21,440 --> 00:15:25,200
+القطبية إذا الـ stationary polar إذا المواد ال
+
+217
+00:15:25,200 --> 00:15:28,260
+polar هتضلها لزجة فيه والمواد الـ non-polar هتطلع 
+
+218
+00:15:28,260 --> 00:15:32,630
+لفوق إذا الـ stationary non-polar إذا المواد الـ
+
+219
+00:15:32,630 --> 00:15:38,060
+non-polar بتضلها لزجة فيه و الـ polar بيطلع لفوق
+
+220
+00:15:38,060 --> 00:15:40,980
+mixture to be separated is first applied to an
+
+221
+00:15:40,980 --> 00:15:44,440
+immovable porous solid إذا المكتشر الخليط اللي أنا
+
+222
+00:15:44,440 --> 00:15:47,540
+بدي أفصله وين بحطه أول شيء بالبداية بحطه على ال
+
+223
+00:15:47,540 --> 00:15:51,080
+stationary ولا على الـ mobile لأ بأسقطه في ال
+
+224
+00:15:51,080 --> 00:15:54,200
+stationary phase في البرة ال solid material اللي
+
+225
+00:15:54,200 --> 00:15:57,720
+هي المادة المثقبة اللي بتكون عبارة عن ورقة حكاية
+
+226
+00:15:57,720 --> 00:16:02,880
+أو ألمنيوم أو سليكا اللي هي اسمها stationary phase
+
+227
+00:16:03,510 --> 00:16:06,430
+the component of the mixture then get washed
+
+228
+00:16:06,430 --> 00:16:10,610
+وبعدين بغمرها حكاية البر الستاشنريال اللي هي ال
+
+229
+00:16:10,610 --> 00:16:14,350
+stationary phase بغمرها في الـ solvent اللي بنسميه
+
+230
+00:16:14,350 --> 00:16:18,990
+mobile phase بعدين الـ mixture بيصير له separated by
+
+231
+00:16:18,990 --> 00:16:22,830
+different بناء على إيش؟ بناء على الـ attraction،
+
+232
+00:16:22,830 --> 00:16:26,850
+بناء على الـ polarity مع الـ stationary phase و ال
+
+233
+00:16:26,850 --> 00:16:30,970
+mobile phase، بناء على أي مبدأ، بناء على مبدأ
+
+234
+00:16:30,970 --> 00:16:34,960
+like dissolves like لذن بعد ما أحط المواد على ال
+
+235
+00:16:34,960 --> 00:16:38,620
+stationary phase بغمرها في الـ mobile phase بيبدأ
+
+236
+00:16:38,620 --> 00:16:41,420
+يصعد الـ mobile phase عبر الـ stationary phase
+
+237
+00:16:41,420 --> 00:16:46,340
+وهيمشي معاه مواد هتمشي معاه مكونات الخليط بناء على
+
+238
+00:16:46,340 --> 00:16:50,220
+إيش، بناء على الـ polarity، بناء على الـ affinity،
+
+239
+00:16:50,220 --> 00:16:54,830
+بناء على الـ attraction التجاذب بينها وبين الـ Face
+
+240
+00:16:54,830 --> 00:16:58,930
+إذا هي والله متحبة و Affinity وقوة تجاذب عالية
+
+241
+00:16:58,930 --> 00:17:02,590
+وخصائصها الـ Polarity نفس خصائص الـ Stationary إذا
+
+242
+00:17:02,590 --> 00:17:05,970
+هتظلها ملزجة في الـ Stationary مش هتصعد للأعلى إذا
+
+243
+00:17:05,970 --> 00:17:09,690
+خصائصها و الـ Affinity و التجاذب قوي مع الـ Mobile
+
+244
+00:17:09,690 --> 00:17:16,270
+Face إذا هي هتصعد مع الـ Mobile Face للأعلى if the
+
+245
+00:17:16,270 --> 00:17:19,330
+stationary phase is polar إذا الـ stationary كانت
+
+246
+00:17:19,330 --> 00:17:22,030
+polar إذا أن الـ polar component المكونات اللي
+
+247
+00:17:22,030 --> 00:17:27,740
+بتكون موجودة في الخليط كانت polar هي هتلزج في ال
+
+248
+00:17:27,740 --> 00:17:32,060
+stationary phase مش هتطلع لفوق و الـ non-polar هي
+
+249
+00:17:32,060 --> 00:17:36,260
+اللي هتطلع أسرع للأعلى لكن لو الـ stationary phase
+
+250
+00:17:36,260 --> 00:17:40,180
+is non-polar إذا الـ non-polar هي اللي هتتجاذب مع
+
+251
+00:17:40,180 --> 00:17:43,840
+الـ stationary و تضلها تحت و الـ polar هو اللي هيصعد
+
+252
+00:17:43,840 --> 00:17:50,860
+للأعلى بقوة أعلى أو بواجهة أقل الـ separation
+
+253
+00:17:50,860 --> 00:17:54,680
+depends on several factors عملية الفصل بتعتمد على
+
+254
+00:17:54,680 --> 00:17:59,640
+عدة عوامل عملية الفصل بتعتمد على عدة عوامل في
+
+255
+00:17:59,640 --> 00:18:03,620
+عندنا عاملين رئيسيين وفي عندنا الثالث أول عامل هي
+
+256
+00:18:03,620 --> 00:18:10,060
+solubility solubility with mobile phase أول عامل الـ
+
+257
+00:18:10,060 --> 00:18:13,400
+solubility with mobile phase اللي مكتوب هنا الـ
+
+258
+00:18:13,400 --> 00:18:16,460
+solvent الـ solvent معناه الـ mobile phase إذا كان
+
+259
+00:18:16,460 --> 00:18:19,780
+بيدوب في الـ mobile phase له نفس خصائص الـ mobile
+
+260
+00:18:19,780 --> 00:18:24,520
+phase إذا هو هيصعد للأعلى العامل الثاني ال
+
+261
+00:18:24,520 --> 00:18:29,350
+attraction between compound and silica اللي هي قوة
+
+262
+00:18:29,350 --> 00:18:32,570
+التجاذب بين المادة اللي موجودة في الخليط والسيليكا
+
+263
+00:18:32,570 --> 00:18:35,450
+اللي هي الـ stationary phase الـ attraction يعني
+
+264
+00:18:35,450 --> 00:18:39,070
+التجاذب مع الـ stationary phase إذا هي متجاذبة أو
+
+265
+00:18:39,070 --> 00:18:44,410
+حابة للـ stationary إذا هتظلها في الأسفل إذا أول
+
+266
+00:18:44,410 --> 00:18:47,550
+عاملين رئيسيين يعتمد عليهم تقنية الفصل هي
+
+267
+00:18:47,550 --> 00:18:51,920
+solubility with mobile phase والـ Attraction with
+
+268
+00:18:51,920 --> 00:18:56,120
+stationary phase وهذا هو نفسه الـ principle لما
+
+269
+00:18:56,120 --> 00:19:00,100
+أعمل اليوم أنه أنا بدي أعمل فصل لخريطة من مكونات
+
+270
+00:19:00,100 --> 00:19:03,920
+من عدة مكونات بناء على إيش بده يفصل، بناء على أي
+
+271
+00:19:03,920 --> 00:19:07,900
+مبدأ، بناء على مبدأ الـ solubility with mobile phase
+
+272
+00:19:07,900 --> 00:19:12,760
+أنه مدى الذائبية مع الـ mobile phase و الـ attraction
+
+273
+00:19:12,760 --> 00:19:16,820
+مع الـ stationary phase ومدى التجاذب مع الـ
+
+274
+00:19:16,820 --> 00:19:19,380
+stationary phase والله إذا هي متجاذبة مع ال
+
+275
+00:19:19,380 --> 00:19:22,780
+stationary phase هتضلها لتحت ومش هتطلع إذا هي
+
+276
+00:19:22,780 --> 00:19:25,100
+solubility مع الـ mobile phase اللي هي عالية
+
+277
+00:19:25,100 --> 00:19:29,280
+وذائبة في الـ mobile phase إذا هتصعد للأعلى ثالث
+
+278
+00:19:29,280 --> 00:19:34,310
+عامل هو الـ structure of compound ليه الحجم؟ الثالث
+
+279
+00:19:34,310 --> 00:19:38,570
+عامل يقصد فيه الـ size حجم المادة اللي موجودة في
+
+280
+00:19:38,570 --> 00:19:42,930
+الخليط اللي بدي أفصلها إذا حجمها كبير هتمشي ببطء
+
+281
+00:19:42,930 --> 00:19:47,830
+هتكون بطيئة في المشي وبالتالي مش هتمشي كثير هتكون
+
+282
+00:19:47,830 --> 00:19:53,330
+ملزقة لتحت أكثر لكن إذا كان حجمها صغير هتمشي أسرع
+
+283
+00:19:55,830 --> 00:20:00,770
+إيش مميزات الـ chromatography الـ advantage of
+
+284
+00:20:00,770 --> 00:20:04,650
+chromatography أول حاجة كان separate complex
+
+285
+00:20:04,650 --> 00:20:08,570
+mixture with greater precision أنها بتفصل خليط من
+
+286
+00:20:08,570 --> 00:20:14,490
+عدة مكونات بدقة عالية بتفصل خليط بدقة عالية يعني
+
+287
+00:20:14,490 --> 00:20:20,600
+لو في مادتين متشابهات ولكن بيختلفوا زي البروتين
+
+288
+00:20:20,600 --> 00:20:23,540
+مثلا هو عبارة عن سلسلة من أحماض أمينية لو في عندي
+
+289
+00:20:23,540 --> 00:20:27,320
+نوعين من البروتين هما نفس سلسلة أحماض الأمينية بس
+
+290
+00:20:27,320 --> 00:20:31,700
+بيختلفوا بحمض أميني واحد بتقدر تفصلوا هذه التقنية
+
+291
+00:20:31,700 --> 00:20:36,350
+تفصلهم عن بعض Can purify any soluble or volatile
+
+292
+00:20:36,350 --> 00:20:39,270
+substance if the right adsorbent material, carrier
+
+293
+00:20:39,270 --> 00:20:42,930
+fluid and operating condition are employed بتقدر
+
+294
+00:20:42,930 --> 00:20:49,930
+تعمل تنقية لأي خليط من أي مواد، من عدة مواد تقدر
+
+295
+00:20:49,930 --> 00:20:53,930
+تنقي الخليط، أنه تخلي كل مكون لحاله، بشرط أن
+
+296
+00:20:53,930 --> 00:20:57,630
+أستخدم الـ stationary phase المناسب، الـ mobile
+
+297
+00:20:57,630 --> 00:21:01,930
+phase المناسب والتقنيات والخطوات إجراء التجربة
+
+298
+00:21:01,930 --> 00:21:07,280
+الصحيحة exact quantitative analysis is done even
+
+299
+00:21:07,280 --> 00:21:11,240
+from a trace compound بقدر أعمل منها quantitative
+
+300
+00:21:11,240 --> 00:21:16,560
+analysis اللي هو تحليل كمي أعرف كمية or small
+
+301
+00:21:16,560 --> 00:21:19,780
+material consumption اللي هو هذه التقنية بتستهلكش
+
+302
+00:21:19,780 --> 00:21:24,920
+مواد كثيرة، مواد بسيطة the quantitation has a broad
+
+303
+00:21:24,920 --> 00:21:30,200
+linearity range لها مدى واسع، لها مدى واسع من
+
+304
+00:21:30,200 --> 00:21:35,680
+المواد اللي تقدر تفصلها sixth analyze of several
+
+305
+00:21:35,680 --> 00:21:39,720
+compounds can be done during one run بقدر أفصل
+
+306
+00:21:39,720 --> 00:21:43,280
+أكثر من خليط مرة واحدة يعني بجيب الـ stationary
+
+307
+00:21:43,280 --> 00:21:47,100
+phase اللي بده أحط عليه الخليط بقى بحط خليط في
+
+308
+00:21:47,100 --> 00:21:50,580
+مكان بحط نقطة من هذا الخليط في مكان ثاني نقطة من
+
+309
+00:21:50,580 --> 00:21:55,420
+الخليط الثاني في نفس الورقة بقدر أفصل عدة خليط عدة
+
+310
+00:21:55,420 --> 00:21:59,840
+أنواع من الخليط ممكن خليط اثنين ثلاثة في نفس ال
+
+311
+00:21:59,840 --> 00:22:05,130
+stationary phase في نفس التقنية في نفس المرة ال
+
+312
+00:22:05,130 --> 00:22:07,930
+chromatography is a fast analysis method هي طريقة
+
+313
+00:22:07,930 --> 00:22:12,650
+سريعة وآخر شيء أنه حاليا صارت instrumentation
+
+314
+00:22:12,650 --> 00:22:15,910
+صارت عبارة عن أجهزة صارت automation بدل ما نعملها
+
+315
+00:22:15,910 --> 00:22:20,190
+بشكل يدوي أتمت تقنية الفصل باستخدام أجهزة
+
+316
+00:22:22,690 --> 00:22:26,430
+في عندنا سؤال is this a pure substance or mixture؟
+
+317
+00:22:26,430 --> 00:22:31,270
+أنه هل المادة اللي عندي هي مادة عبارة عن مادة نقية
+
+318
+00:22:31,270 --> 00:22:34,890
+بتتكون من مكون واحد ولا هي عبارة عن خليط كيف
+
+319
+00:22:34,890 --> 00:22:39,170
+بتعرف؟ أنا أعطيتك مادة وحكيت لك عرفيها هل هي pure
+
+320
+00:22:39,170 --> 00:22:43,090
+substance ولا mixture؟ كيف بدك تعرفي؟ بيحكي لي
+
+321
+00:22:43,090 --> 00:22:46,830
+بنشتغل تقنية الـ chromatography بشكل عام، هذا ملخص
+
+322
+00:22:46,830 --> 00:22:49,670
+للـ slide هاي أنه أنا بدي أشتغل تقنية ال
+
+323
+00:22:49,670 --> 00:22:54,030
+chromatography هنكتب للـ TLC TLC هو نوع من أنواع
+
+324
+00:22:54,030 --> 00:22:58,790
+الكروماتوجرافي هنتعرف عليه لقدام هي اختصارها thin
+
+325
+00:22:58,790 --> 00:23:04,560
+layer chromatography TLC Thin Layer Chromatography
+
+326
+00:23:04,560 --> 00:23:07,800
+بشكل عام أنا كيف بده يعرف بده يجاوب عليها ده
+
+327
+00:23:07,800 --> 00:23:12,140
+السؤال هل هي pure مادة نقية ولا خليط بدي أشتغل
+
+328
+00:23:12,140 --> 00:23:16,820
+تقنية الـ chromatography وأشتغلها بعد ما يفصل الخليط
+
+329
+00:23:16,820 --> 00:23:19,520
+أو يمشي الـ mobile phase عبر الـ stationary phase
+
+330
+00:23:19,520 --> 00:23:24,300
+إذا أعطاني نقطة واحدة أنه في مكان واحد نقطة واحدة
+
+331
+00:23:24,300 --> 00:23:28,520
+إذا هذه pure substance مادة نقية لأنه ما أعطانيش
+
+332
+00:23:28,520 --> 00:23:33,380
+غير مكون واحد إذا أعطاني أكثر من نقطة في أكثر من
+
+333
+00:23:33,380 --> 00:23:38,610
+مكان فهذا معناه أنه كان mixture كان عبارة عن خليط
+
+334
+00:23:38,610 --> 00:23:43,370
+وفصل إلى عدة مكونات وبالتالي أنا بقدر أعرف ال
+
+335
+00:23:43,370 --> 00:23:47,030
+number of components in a mixture بقدر أعرف عدد
+
+336
+00:23:47,030 --> 00:23:50,850
+مكونات الخليط كيف بده يعرف عدد مكونات الخليط؟ لأنه
+
+337
+00:23:50,850 --> 00:23:55,290
+بعد ما يفصل كم نقطة أعطاني مثلا إذا أعطاني ثلاثة
+
+338
+00:23:55,290 --> 00:23:58,970
+إذا هو عبارة عن ثلاثة مكونات إذا أعطاني بس نقطتين
+
+339
+00:23:58,970 --> 00:24:04,420
+إذا هو عبارة عن مكونين طيب are these compounds
+
+340
+00:24:04,420 --> 00:24:07,940
+identical؟ السؤال الثاني أنه بيحكي لي أن أنا أعرف
+
+341
+00:24:07,940 --> 00:24:15,300
+هل هذول المواد identical شبه بعض أنه عندك مادتين و
+
+342
+00:24:15,300 --> 00:24:19,840
+مش عارفين هل هم نفس المادة محطوطين في مكانين ولا
+
+343
+00:24:19,840 --> 00:24:23,700
+هم مادتين مختلفتين كيف بدك تعرفي؟ برضه بيحكي لي
+
+344
+00:24:23,700 --> 00:24:26,900
+الإجابة أني بدي أشتغل الـ chromatographic technique
+
+345
+00:24:26,900 --> 00:24:32,930
+بدي أشتغل تقنية الـ chromatography بأخذ من المادة
+
+346
+00:24:32,930 --> 00:24:35,750
+الأولى و بحطها على الـ stationery phase والمادة
+
+347
+00:24:35,750 --> 00:24:38,470
+الثانية و بحطها برضه على الجانب الثاني في نفس
+
+348
+00:24:38,470 --> 00:24:41,810
+الورقة كل واحدة في مكان و بحطها في الـ mobile phase
+
+349
+00:24:41,810 --> 00:24:45,730
+بغمرها و بيبدأ يفصل بيحكي لي إذا فصلوا النقطتين
+
+350
+00:24:45,730 --> 00:24:50,130
+مقابل بعض في نفس المكان مقابل بعض إذا هم نفس
+
+351
+00:24:50,130 --> 00:24:55,230
+المادة إذا فصلوا كل واحدة في مكان إذا هم مادتين
+
+352
+00:24:55,230 --> 00:25:01,190
+مختلفتين إذا أنا هشتغل الاثنين و أعملهم فصل إذا تم
+
+353
+00:25:01,190 --> 00:25:08,390
+الفصل في نفس المكان إذا هو عبارة عن identical
+
+354
+00:25:08,390 --> 00:25:12,710
+substance أما إذا فصلوا كل واحدة في مكان بيكونوا
+
+355
+00:25:12,710 --> 00:25:17,990
+مواد مختلفة وكيف كمان بدي أعرف الزيادة؟ نحسب حاجة
+
+356
+00:25:18,110 --> 00:25:22,510
+الـ retention factor هنتعرف عليه لقدام لكن أنا 
+
+357
+00:25:22,510 --> 00:25:25,970
+بشكل عام كيف بدي أعرف هل هي identical ولا لأ نفس
+
+358
+00:25:25,970 --> 00:25:30,770
+المادة أشتغل تقنية الـ chromatography إذا فصل
+
+359
+00:25:30,770 --> 00:25:36,050
+المكونين جنب بعض في نفس المكان على الورقة مقابل
+
+360
+00:25:36,050 --> 00:25:40,170
+بعض إذا هم نفس المادة إذا كل واحدة في مكان إذا هم
+
+361
+00:25:40,170 --> 00:25:47,310
+مادتين مختلفتين types of chromatography أقسام ال
+
+362
+00:25:47,310 --> 00:25:50,230
+chromatography لجهة الـ chromatography مقسم إلى
+
+363
+00:25:50,230 --> 00:25:54,690
+قسمين بناء على الـ stationary و بناء على الـ mobile
+
+364
+00:25:54,690 --> 00:25:59,110
+phase الـ chromatography مقسم إلى جزئين بناء على ال
+
+365
+00:25:59,110 --> 00:26:02,190
+stationary إليه تقسيم و بناء على الـ mobile إليه
+
+366
+00:26:02,190 --> 00:26:05,670
+تقسيم بناء على الـ mobile phase فيه نوعين إما
+
+367
+00:26:05,670 --> 00:26:09,250
+بستخدم الـ mobile phase يكون gas، غاز أو يكون
+
+368
+00:26:09,250 --> 00:26:13,230
+liquid بناء على الـ stationary phase في منه column
+
+369
+00:26:13,230 --> 00:26:17,510
+على شكل عمود وفي منه planar على شكل سطح مستوي
+
+370
+00:26:17,510 --> 00:26:21,130
+إذا بناء على الـ stationary phase في منه اثنين
+
+371
+00:26:21,130 --> 00:26:25,570
+column عمود أو planar مستوي و الـ planar في منه
+
+372
+00:26:25,570 --> 00:26:30,150
+thin layer chromatography وفي منه paper
+
+373
+00:26:30,150 --> 00:26:36,110
+chromatography هنا جدول بيوضح أنه أو أنه ملخص هاي
+
+374
+00:26:36,110 --> 00:26:38,790
+الـ slide المطلوب منها أصلا منها أنه ال
+
+375
+00:26:38,790 --> 00:26:41,890
+chromatography تقسيم الـ chromatography بناء على ال
+
+376
+00:26:41,890 --> 00:26:45,510
+mobile phase أن في عندنا liquid chromatography و
+
+377
+00:26:45,510 --> 00:26:49,610
+gas chromatography بس لذا تقسيم الـ chromatography
+
+378
+00:26:49,610 --> 00:26:53,010
+بناء على الـ mobile phase في منها liquid وفي منها
+
+379
+00:26:53,010 --> 00:26:57,780
+gas الـ classification أما بناء على الـ stationary
+
+380
+00:
+
+401
+00:28:29,330 --> 00:28:34,650
+فهي مدعمة بسطح من جلاس plastic أو aluminium 
+
+402
+00:28:34,650 --> 00:28:38,690
+أو plastic أو aluminium ال paper chromatography
+
+403
+00:28:38,690 --> 00:28:41,930
+paper chromatography ال stationary phase بيكون
+
+404
+00:28:41,930 --> 00:28:45,570
+عبارة عن paper عبارة عن ورقة أما الجزء الثاني هو
+
+405
+00:28:45,570 --> 00:28:49,310
+ال column chromatography اللي بيكون عبارة عن عمود
+
+406
+00:28:49,310 --> 00:28:54,860
+فال stationary phase بيكون column طيب ليش بنستخدم
+
+407
+00:28:54,860 --> 00:28:57,480
+تقنية ال chromatography؟ بيحكينا ال
+
+408
+00:28:57,480 --> 00:29:00,580
+chromatographic technique ممكن نستخدمها ك
+
+409
+00:29:00,580 --> 00:29:06,820
+qualitative و ك quantitative ممكن أستخدمها ك
+
+410
+00:29:06,820 --> 00:29:11,480
+qualitative و quantitative ايش يعني qualitative؟
+
+411
+00:29:11,480 --> 00:29:14,740
+يعني أعرف نوع المادة، أعرف اسم المادة، مين هذه
+
+412
+00:29:14,740 --> 00:29:20,370
+المادة Qualitative معناها جودة أو نوع المادة تحديد
+
+413
+00:29:20,370 --> 00:29:24,610
+نوع المادة كيف بدي أُحدد نوع المادة بناء على حاجة
+
+414
+00:29:24,610 --> 00:29:29,450
+اسمها ال retention factor أنه أنا بحط خليط من
+
+415
+00:29:29,450 --> 00:29:32,990
+المواد على ال stationary phase و بحطها بغمرها بال
+
+416
+00:29:32,990 --> 00:29:37,650
+mobile phase و بيبدأ يفصل بيفصل كل مكون إلى نقاط
+
+417
+00:29:37,650 --> 00:29:43,170
+كل نقطة في مكان أجيّت وين وصلت هذه النقطة؟ من عندها
+
+418
+00:29:43,170 --> 00:29:47,670
+بحسب حاجة لكل نقطة بحسب ايش اسمه retention factor
+
+419
+00:29:48,850 --> 00:29:53,310
+اللي هو عامل المحافظة كيف بيُحسب هاد؟ هو موجود في
+
+420
+00:29:53,310 --> 00:29:56,410
+آخر slide بس أنه بشكل عام ال retention factor هو
+
+421
+00:29:56,410 --> 00:30:00,290
+عبارة عن ال distance اللي هي المسافة اللي قطعتها
+
+422
+00:30:00,290 --> 00:30:04,470
+المادة، بكون أنا حاطة خط بداية اللي هو حاطيت من
+
+423
+00:30:04,470 --> 00:30:07,190
+عنده الخليط، النقطة وين أنت حاطيت الخليط، من وين
+
+424
+00:30:07,190 --> 00:30:10,970
+بدأتِ، من هنا أنا حاطيت النقطة، بحسب المسافة اللي 
+
+425
+00:30:10,970 --> 00:30:13,930
+قطعتها المادة من هذا الخط لوين وصلت بعد الفصل
+
+426
+00:30:14,870 --> 00:30:19,730
+المادة وين وصلت بعد الفصل يعني لو رجعنا لهذه
+
+427
+00:30:19,730 --> 00:30:25,790
+الصورة هل جت المواد بعد الفصل مثلًا الحمرة أنتِ حطيتِ
+
+428
+00:30:25,790 --> 00:30:29,350
+الخليط وين؟ من تحت من خط البداية من تحت صح؟ بدي 
+
+429
+00:30:29,350 --> 00:30:32,330
+أحسب المسافة من خط البداية اللي عند الحمرة بدي
+
+430
+00:30:32,330 --> 00:30:37,580
+أُقيس بالمسطرة جدّيش قطعت الحمرة مسافة تمام؟ على
+
+431
+00:30:37,580 --> 00:30:41,640
+المسافة اللي قطعها الـ mobile phase من البداية
+
+432
+00:30:41,640 --> 00:30:45,300
+لوين وصل يعني الجزء الأزرق السائل الأزرق هاد من
+
+433
+00:30:45,300 --> 00:30:50,160
+وين كان من البداية لفوق لجزء الأزرق لآخر وين وصل
+
+434
+00:30:50,160 --> 00:30:53,220
+هذه المسافة كلها برضه بدّه أقيسها في المسطرة ال
+
+435
+00:30:53,220 --> 00:30:56,340
+retention factor هو عبارة عن المسافة اللي قطعتها
+
+436
+00:30:56,340 --> 00:31:00,760
+المادة من خط البداية على المسافة اللي قطعها ال
+
+437
+00:31:00,760 --> 00:31:05,370
+solvent كامل من خط البداية لعند ما وصل هذه اسمها
+
+438
+00:31:05,370 --> 00:31:09,010
+retention factor إذًا ال retention factor هي
+
+439
+00:31:09,010 --> 00:31:15,850
+المسافة اللي قطعتها المادة أعلى المسافة اللي قطعها
+
+440
+00:31:15,850 --> 00:31:19,190
+ال solvent من خط البداية إلى وين وصل كده بيطلع
+
+441
+00:31:19,190 --> 00:31:23,270
+عندي رقم كل مادة إلها retention factor معروف
+
+442
+00:31:23,270 --> 00:31:27,350
+ومحفوظ في جدول وجدول علماء ومكتوبة في جدول برجع
+
+443
+00:31:27,350 --> 00:31:31,250
+لهذا الجدول بدور على الرقم اللي طلع معاه retention
+
+444
+00:31:31,250 --> 00:31:34,970
+factor اللي طلعت معاه بطلع بروح بدور في الجدول
+
+445
+00:31:34,970 --> 00:31:39,050
+مثلًا ستة بروح أشوف قبل الستة مين هي المادة بس بطلع
+
+446
+00:31:39,050 --> 00:31:43,730
+اسمها و بكون عارفيتها، بعرفها مثلًا نص، بروح للجدول
+
+447
+00:31:43,730 --> 00:31:47,810
+بطلع مين النص؟ مثلًا لل glycine طلعت النص، إذا
+
+448
+00:31:47,810 --> 00:31:51,390
+المادة هي عبارة عن glycine ف qualitative analysis
+
+449
+00:31:51,390 --> 00:31:54,950
+كيف؟ من خلال أني أحسب ال retention factor
+
+450
+00:31:56,250 --> 00:31:59,370
+Quantitative determination برضه بيستخدم ك
+
+451
+00:31:59,370 --> 00:32:04,050
+quantitative quantitative يعني كمية يعني بتعرف
+
+452
+00:32:04,050 --> 00:32:08,390
+كمية المادة الموجودة جدّيش كميتها بيحكي لي هذا بيتم
+
+453
+00:32:08,390 --> 00:32:12,590
+معرفته بناء على ال intensity of a spot وبناء على
+
+454
+00:32:12,590 --> 00:32:17,110
+ال size of the spot بناء على شدة ال only spot هي
+
+455
+00:32:17,110 --> 00:32:20,110
+اللي جيت بعد ما يفصل المواد وبيعطينيها على شكل
+
+456
+00:32:20,110 --> 00:32:25,010
+نقاط spot بنسميها نقاط هذه النقطة جدّيش حجمها
+
+457
+00:32:25,010 --> 00:32:29,050
+الدائرة اللي طلعت معي جدّيش حجمها اللي هي صغيرة
+
+458
+00:32:29,050 --> 00:32:33,750
+ولا كبيرة طب الشدة قديش هي واضحة، نغامجة ولا إنها 
+
+459
+00:32:33,750 --> 00:32:38,710
+خفيفة؟ هذه بتحدد لكمية المادة إذا كان مثلًا حجمها
+
+460
+00:32:38,710 --> 00:32:43,110
+كبير إذا هي مادة موجودة بكمية كبيرة إذا حجمها صغير
+
+461
+00:32:43,110 --> 00:32:47,570
+موجودة بكمية أقل تمام، بدي أعرف بزيادة ال
+
+462
+00:32:47,570 --> 00:32:51,010
+quantitative analysis بيستخدموا جهاز اسمه الـ
+
+463
+00:32:51,010 --> 00:32:57,770
+densitometer جهاز اسمه densitometer يستخدم في تقنية
+
+464
+00:32:57,770 --> 00:33:02,090
+ال chromatography for quantitative determination
+
+465
+00:33:02,090 --> 00:33:07,490
+يعني هو جهاز instrument to detect the color .. the
+
+466
+00:33:07,490 --> 00:33:12,330
+intensity of color هو جهاز يستخدم لمقياس شدة لون
+
+467
+00:33:12,330 --> 00:33:15,810
+للبقعة اللي ظهرت عندي في تقنية ال chromatography
+
+468
+00:33:15,810 --> 00:33:21,750
+عشان يعطيني quantitative determination للمادة إذا
+
+469
+00:33:21,750 --> 00:33:25,490
+بيستخدم التقنية الـ chromatography for qualitative
+
+470
+00:33:25,490 --> 00:33:33,150
+و quantitative للكمي و الجودة paper chromatography
+
+471
+00:33:33,150 --> 00:33:36,450
+ال paper احنا اليوم هنستخدم paper chromatography
+
+472
+00:33:36,450 --> 00:33:39,430
+ال paper chromatography حكينا إن ال stationary
+
+473
+00:33:39,430 --> 00:33:43,230
+phase عبارة عن paper و ال solvent ال mobile phase
+
+474
+00:33:43,230 --> 00:33:47,410
+بيكون liquid ال paper chromatography بحط عليها
+
+475
+00:33:47,410 --> 00:33:50,670
+العينات بحمل عليها العينات إن أنا بدي أحط الخليط
+
+476
+00:33:50,670 --> 00:33:53,930
+من المواد على ال paper على ال stationary phase على
+
+477
+00:33:53,930 --> 00:33:57,430
+هيئة ال spot بحط نقطة من هذا الخليط على هذه الورقة
+
+478
+00:33:57,970 --> 00:34:01,410
+بعدين الورقة بغمرها في ال solvent بغمرها dipped
+
+479
+00:34:01,410 --> 00:34:06,130
+into a solvent بغمرها في ال mobile phase بيبدأ
+
+480
+00:34:06,130 --> 00:34:09,410
+يطلع يمشي ال mobile phase عبر ال stationary phase
+
+481
+00:34:09,410 --> 00:34:13,570
+بيمشي السائل للأعلى بناء على خاصية ال capillary
+
+482
+00:34:13,570 --> 00:34:19,050
+action الخاصية الشعرية و طول ما هو ماشي السائل
+
+483
+00:34:19,050 --> 00:34:22,770
+بتنفصل الخليط إلى مكوناته بناء على ايش حكينا
+
+484
+00:34:22,770 --> 00:34:26,950
+الفاصل؟ بناء على ال solubility و ال attraction مدى
+
+485
+00:34:26,950 --> 00:34:31,050
+ذائبيتها في الـ Mobile Phase ومدى تجاذبها مع الـ
+
+486
+00:34:31,050 --> 00:34:36,450
+Stationary Phase إذا أنا ده تكرار الكلام كله الـ
+
+487
+00:34:36,450 --> 00:34:39,790
+principle of paper chromatography الـ capillary
+
+488
+00:34:39,790 --> 00:34:42,770
+action هي شيء هي movement of liquid within the
+
+489
+00:34:42,770 --> 00:34:47,490
+space of a porous material هي حركة ال mobile phase
+
+490
+00:34:47,490 --> 00:34:50,710
+عبر ال stationary phase ال capillary action هي
+
+491
+00:34:50,710 --> 00:34:53,410
+عبارة عن movement of liquid اللي هو ال mobile
+
+492
+00:34:53,410 --> 00:34:56,570
+phase within a porous material اللي هي ال
+
+493
+00:34:56,570 --> 00:35:00,430
+stationary phase بس بتعبير آخر هي حركة ال mobile
+
+494
+00:35:00,430 --> 00:35:04,890
+phase عبر ال stationary phase الـ Liquid اللي هو
+
+495
+00:35:04,890 --> 00:35:08,230
+الـ mobile phase بيتحرك عبر ال paper بسبب أن
+
+496
+00:35:08,230 --> 00:35:12,850
+الجاذبية ال attraction التجاذب أقوى من قوة
+
+497
+00:35:12,850 --> 00:35:18,860
+الجاذبية بناء على أي مبدأ هيتم الفصل وبناء على ايش
+
+498
+00:35:18,860 --> 00:35:22,760
+حكينا بناء على like dissolve like وبناء على ال
+
+499
+00:35:22,760 --> 00:35:26,900
+solubility مع ال mobile phase و ال affinity أو ال
+
+500
+00:35:26,900 --> 00:35:30,940
+attraction with stationary phase إذا ال principle
+
+501
+00:35:30,940 --> 00:35:36,080
+لو بدي أُلخصه هو حاجتين ال affinity with mobile ال
+
+502
+00:35:36,080 --> 00:35:39,740
+affinity أو attraction with stationary phase و
+
+503
+00:35:39,740 --> 00:35:45,480
+solubility with mobile phase الـ Thin Layer
+
+504
+00:35:45,480 --> 00:35:51,280
+Chromatography هي تقنية لفصل المواد بيكون فيها الـ
+
+505
+00:35:51,280 --> 00:35:55,260
+stationary phase عبارة عن Thin Layer عبارة عن طبقة
+
+506
+00:35:55,260 --> 00:35:59,940
+خفيفة رفيعة of silica بتكون عبارة عن هذه الطبقة
+
+507
+00:35:59,940 --> 00:36:05,300
+سيليكا أو ألمنيوم سيليكا مدعمة بسطح من جلاس أو ألمنيوم
+
+508
+00:36:05,300 --> 00:36:09,950
+برضه لتدعيم إذا الـ thin layer chromatography هي
+
+509
+00:36:09,950 --> 00:36:13,350
+عبارة عن سيليكا بيكون ال stationary phase ورقة
+
+510
+00:36:13,350 --> 00:36:18,870
+رفيعة من السيليكا مدعمة بسطح من جلاس أو ألمنيوم السيليكا
+
+511
+00:36:18,870 --> 00:36:22,350
+جيل هتكون هي ال stationary phase أما ال solvent هو
+
+512
+00:36:22,350 --> 00:36:25,650
+ال mobile phase إذا ال thin layer chromatography
+
+513
+00:36:25,650 --> 00:36:29,770
+بدك تكون عارفة إن هي عبارة عن سيليكا ال stationary
+
+514
+00:36:29,770 --> 00:36:33,770
+phase عبارة عن سيليكا و ال solvent هو ال mobile
+
+515
+00:36:33,770 --> 00:36:40,250
+phase ايش مبدأ الفصل ال principle ملخص ال principle
+
+516
+00:36:40,250 --> 00:36:44,670
+كله حكيناه إنه عبارة عن affinity with stationary
+
+517
+00:36:44,670 --> 00:36:50,570
+phase و attraction with mobile with stationary
+
+518
+00:36:50,570 --> 00:36:53,650
+phase و solubility with mobile phase أجيّت هذا
+
+519
+00:36:53,650 --> 00:36:57,030
+الكلام اللي أجيّت مكتوب في ال principle كله تكرار
+
+520
+00:36:57,030 --> 00:37:00,530
+للي قلناها different compound in sample mixture
+
+521
+00:37:00,530 --> 00:37:03,030
+travel different distance according to how
+
+522
+00:37:03,030 --> 00:37:06,470
+strongly they interact with the stationary phase
+
+523
+00:37:06,470 --> 00:37:10,370
+and mobile phase بيحكي لي المواد بتمشي اللي موجودة
+
+524
+00:37:10,370 --> 00:37:14,570
+في الخلط بتنفصلها و بتمشي مسافات مختلفة كل واحدة
+
+525
+00:37:14,570 --> 00:37:18,750
+بتقف في مكان في مسافة مختلفة في مكان مختلف بناء
+
+526
+00:37:18,750 --> 00:37:21,910
+على ال attraction بناء على ال interact بناء على
+
+527
+00:37:21,910 --> 00:37:25,770
+التجادب مع ال stationary phase و ال mobile phase
+
+528
+00:37:31,430 --> 00:37:37,310
+المواد اللي موجودة في الخليط بتختلف في قوة ذائبية
+
+529
+00:37:37,310 --> 00:37:41,470
+والالتصاق مع ال stationary أو مع ال mobile phase
+
+530
+00:37:41,470 --> 00:37:44,850
+ال mobile phase اللي هو ال liquid أو ال eluent
+
+531
+00:37:44,850 --> 00:37:46,110
+نفس المسمى
+
+532
+00:37:49,820 --> 00:37:53,080
+وهو يرتفع ببطء بيقول إن السائل اللي هو الـ mobile
+
+533
+00:37:53,080 --> 00:37:57,280
+phase بيمشي عبر الـ stationary phase اللي موجود في
+
+534
+00:37:57,280 --> 00:38:00,580
+الـ thin layer كروماتوجرافي بالخاصية الشعرية بالـ
+
+535
+00:38:00,580 --> 00:38:04,680
+capillary action separation of compound is based
+
+536
+00:38:04,680 --> 00:38:07,940
+on the competition of the solute and the mobile
+
+537
+00:38:07,940 --> 00:38:11,740
+phase for binding substances بيقول لي إن فصل
+
+538
+00:38:11,740 --> 00:38:15,860
+المواد بناء على التنافس بين ال mobile phase و ال
+
+539
+00:38:15,860 --> 00:38:19,680
+stationary phase على هذه المواد مين ياخذ مواد أكثر
+
+540
+00:38:19,680 --> 00:38:24,180
+وهذا بناء على solubility أنا جيت اللي احنا
+
+541
+00:38:24,180 --> 00:38:27,840
+بنستخدمها هي thin layer chromatography و الـ thin
+
+542
+00:38:27,840 --> 00:38:33,040
+layer حكينا إنه عبارة عن silica gel و السيليكا هي
+
+543
+00:38:33,040 --> 00:38:36,260
+polar فبيحكي لي if normal phase إذا احنا استخدمنا
+
+544
+00:38:36,260 --> 00:38:40,040
+silica gel as stationary phase ال stationary phase
+
+545
+00:38:40,040 --> 00:38:43,880
+يكون عندنا silica gel فهو polar ف أنا لو حطيت
+
+546
+00:38:43,880 --> 00:38:48,160
+مادتين بيختلفوا في ال polarity تمام؟ المادة اللي
+
+547
+00:38:48,160 --> 00:38:51,620
+هتكون Polar، More Polar، Stronger Attraction
+
+548
+00:38:51,620 --> 00:38:55,740
+والسيلكا، مدام خصائصها Polar، المادة هي Polar، إذا 
+
+549
+00:38:55,740 --> 00:38:59,080
+إن بتشبه خصائص السيلكا، هي Polar زيها، إذا إنّها
+
+550
+00:38:59,080 --> 00:39:02,800
+تلزق في الـstationary phase في السيلكا ومش هتمشي
+
+551
+00:39:02,800 --> 00:39:07,160
+للأعلى أما المواد الـNon Polar هي اللي هتمشي
+
+552
+00:39:07,160 --> 00:39:11,890
+لأعلى، move higher up to the plate فهتمشي أعلى
+
+553
+00:39:11,890 --> 00:39:16,850
+الأسرع للأعلى مع ال mobile phase لأن خصائصها تشبه
+
+554
+00:39:16,850 --> 00:39:20,270
+خصائص ال mobile هي non polar زي ال mobile لأن ال
+
+555
+00:39:20,270 --> 00:39:25,850
+stationary silica وهي polar فهذا
+
+556
+00:39:25,850 --> 00:39:29,530
+ال principle كله كلام زي اللي حكيناه ملخص ال
+
+557
+00:39:29,530 --> 00:39:34,490
+principle كله بكلمتين هي عبارة عن attraction with
+
+558
+00:39:34,490 --> 00:39:38,610
+stationary phase و solubility with mobile phase
+
+559
+00:39:40,250 --> 00:39:43,730
+retention factor حكينا عنه هو العامل المحافظة
+
+560
+00:39:43,730 --> 00:39:48,390
+بيحكي إنه كل مادة كيميائية لها retention factor
+
+561
+00:39:48,390 --> 00:39:54,830
+خاص فيها من خلالها بعرف هذه المادة كل مادة لها
+
+562
+00:39:54,830 --> 00:39:58,570
+retention factor خاص فيها أنا بحسب retention
+
+563
+00:39:58,570 --> 00:40:02,330
+factor بعد ما تفصل المواد بحسب لكل مادة retention
+
+564
+00:40:02,330 --> 00:40:06,520
+factor حكينا قانون ال retention factor ايش هو هو
+
+565
+00:40:06,520 --> 00:40:10,940
+المسافة اللي قطعتها المادة من البداية لوين وصلت على
+
+566
+00:40:10,940 --> 00:40:14,280
+المسافة اللي قطعها ال solvent ال mobile phase من
+
+567
+00:40:14,280 --> 00:40:19,160
+البداية لوين وصلت زي ما أنتم شايفين هنا مثلًا أجد
+
+568
+00:40:19,160 --> 00:40:21,980
+خط البداية اللي هو تحت أنا حاطيت الخليط من الخط
+
+569
+00:40:21,980 --> 00:40:26,200
+اللي تحت النقطة الحمراء مثلًا بُقيس المسافة بالمسطرة
+
+570
+00:40:26,200 --> 00:40:30,320
+من البداية لوين هي وصلت على المسافة اللي مشيها
+
+571
+00:40:30,320 --> 00:40:35,520
+المحلول من خط البداية للنهاية وبشوفها بُقيسها على بعض
+
+572
+00:40:35,520 --> 00:40:39,000
+هذا الناتج وبيطلع عندي هذا ال retention factor
+
+573
+00:40:39,000 --> 00:40:47,020
+برجع للجدول المعروف و بشوف جدّيش لكل مادة the
+
+574
+00:40:47,020 --> 00:40:49,680
+stationary phase in thin layer chromatography نيجي
+
+575
+00:40:49,680 --> 00:40:54,080
+نحكي عن ال thin layer chromatography الـ Thin Layer
+
+576
+00:40:54,080 --> 00:40:56,600
+Chromatography حكينا إن الـ Stationary Phase هي
+
+577
+00:40:56,600 --> 00:41:00,220
+عبارة عن Silica Gel و Silica Gel عبارة عن Polar
+
+578
+00:41:00,220 --> 00:41:03,860
+نيجي نتعرف على Silica Gel بيحكي إن Silica Gel is a
+
+579
+00:41:03,860 --> 00:41:06,000
+form of Silicon Dioxide هي عبارة عن Silicon
+
+580
+00:41:06,000 --> 00:41:10,900
+Dioxide بتكون من Silicon أتم من ذرات السيليكون
+
+581
+00:41:10,900 --> 00:41:15,560
+مرتبطة بجزيئات أكسجين ال structure الموجود فوق في
+
+582
+00:41:15,560 --> 00:41:19,220
+أقصى اليمين في
+
+601
+00:42:38,400 --> 00:42:41,380
+stationary phase عندك 10 layer chromatography و
+
+602
+00:42:41,380 --> 00:42:45,700
+عندك مادة A و مادة B A كانت polar و B non polar
+
+603
+00:42:45,700 --> 00:42:50,420
+مين اللي هتمشي إلى الأعلى أكتر؟ إذا أنا بدي
+
+604
+00:42:50,420 --> 00:42:53,080
+أفهم لحالي 10 layer chromatography إذا ال
+
+605
+00:42:53,080 --> 00:42:57,420
+stationary phase بدي يكون polar فالمادة A إذا كانت
+
+606
+00:42:57,420 --> 00:43:03,300
+هي ال polar هي اللي هتمشي إذا المادة A هي ال polar
+
+607
+00:43:03,300 --> 00:43:07,640
+إذا أن هي مش هتمشي هي اللي هتضلها لتحت بس إذا 
+
+608
+00:43:07,640 --> 00:43:12,600
+المادة B ال non polar هي اللي هتمشي لفوق لأنه 
+
+609
+00:43:12,600 --> 00:43:15,060
+thin layer chromatography بتتفاعل مع طول ال
+
+610
+00:43:15,060 --> 00:43:19,020
+stationary phase بيكون polar فالمادة ال polar
+
+611
+00:43:19,020 --> 00:43:22,020
+هتلزق و مش هتمشي للأعلى و المادة ال non polar هي
+
+612
+00:43:22,020 --> 00:43:27,720
+اللي هتمشي للأعلى طب في عندنا تقنية ثانية اسمها
+
+613
+00:43:27,720 --> 00:43:31,480
+Electrophoresis في عندنا احنا اليوم حكينا عن تقنية
+
+614
+00:43:31,480 --> 00:43:34,760
+ال chromatography في عندنا تقنية ثانية اسمها
+
+615
+00:43:34,760 --> 00:43:40,720
+Electrophoresis لنقارن بين التقنيتين بحكيلي بناء
+
+616
+00:43:40,720 --> 00:43:43,360
+على ال definition بناء على التعريف ال
+
+617
+00:43:43,360 --> 00:43:47,540
+chromatography معناها فصل خليط من عدة مكونات
+
+618
+00:43:47,540 --> 00:43:50,260
+بناء على two phases اللي هي ال stationary phase و ال
+
+619
+00:43:50,260 --> 00:43:54,650
+mobile phase إذن تقنية الـ chromatography هي فصل
+
+620
+00:43:54,650 --> 00:43:58,010
+خليط من المواد بناء على ال stationary phase و ال
+
+621
+00:43:58,010 --> 00:44:04,050
+mobile phase أما ال electrophoresis هي فصل خليط من
+
+622
+00:44:04,050 --> 00:44:10,550
+عدة مكونات بناء على الخصائص الكهربائية على ال
+
+623
+00:44:10,550 --> 00:44:14,190
+electrical field هو فصل بناء على الخصائص
+
+624
+00:44:14,190 --> 00:44:16,890
+الكهربائية ال electrophoresis أما ال
+
+625
+00:44:16,890 --> 00:44:20,730
+chromatography هو فصل بناء على stationary phase و
+
+626
+00:44:20,730 --> 00:44:26,720
+mobile phaseالعينة اللي ممكن استخدمها في حالة ال
+
+627
+00:44:26,720 --> 00:44:31,020
+chromatography ايش العينات اللي بقدر أفصلها ممكن
+
+628
+00:44:31,020 --> 00:44:35,120
+أفصل عينات تكون liquid ممكن تكون solid ممكن gas
+
+629
+00:44:35,120 --> 00:44:41,100
+بيقدر يفصل عينات تكون سائلة ممكن صلبة ممكن غازية
+
+630
+00:44:41,100 --> 00:44:47,700
+ال electrophoresis بيقدر يفصل عينات تكون سائلة و
+
+631
+00:44:47,700 --> 00:44:52,710
+صلبة فقط بيقدرش يفصل الغاز الـ Principle المبدأ
+
+632
+00:44:52,710 --> 00:44:55,990
+للكروماتوغرافيا حكى هنا إن المبدأ الكروماتوغرافي الـ
+
+633
+00:44:55,990 --> 00:45:00,170
+Principle هو attraction with stationary phase و
+
+634
+00:45:00,170 --> 00:45:04,150
+solubility with mobile phase أما الـ
+
+635
+00:45:04,150 --> 00:45:07,910
+Electrophoresis هي فصل بناء على الـ electrical
+
+636
+00:45:07,910 --> 00:45:12,550
+field بناء على الخصائص الكهربائية بناء على ال
+
+637
+00:45:12,550 --> 00:45:17,530
+electrical properties إذن هو فصل بناء على الخصائص
+
+638
+00:45:17,530 --> 00:45:21,460
+الكهربائية الـ Principle لأ للـ Electrophoresis
+
+639
+00:45:21,460 --> 00:45:24,840
+بناء على الخصائص الكهربائية بناء على الـ
+
+640
+00:45:24,840 --> 00:45:28,200
+Chromatography بناء على ال attraction with mobile
+
+641
+00:45:28,200 --> 00:45:32,180
+phase with stationary phase with solubility with
+
+642
+00:45:32,180 --> 00:45:33,600
+mobile phase 
diff --git a/PL9fwy3NUQKwZ1Q7zOg1ObgufPMjRI3WY1/EDL86sMHiEI_postprocess.srt b/PL9fwy3NUQKwZ1Q7zOg1ObgufPMjRI3WY1/EDL86sMHiEI_postprocess.srt
new file mode 100644
index 0000000000000000000000000000000000000000..0b993f7cae94f9a405b1b5d9e0be05e82dbf245e
--- /dev/null
+++ b/PL9fwy3NUQKwZ1Q7zOg1ObgufPMjRI3WY1/EDL86sMHiEI_postprocess.srt
@@ -0,0 +1,2568 @@
+1
+00:00:00,490 --> 00:00:04,790
+اليوم ان شاء الله بنحكي في معمل جديد اسمه ال
+
+2
+00:00:04,790 --> 00:00:10,170
+chromatography ال chromatography هي تقنية لفصل
+
+3
+00:00:10,170 --> 00:00:15,630
+الخليط من المواد إلى مكوناتها عندي خليط من المواد
+
+4
+00:00:15,630 --> 00:00:22,170
+من عند المواد لأفصل كل مكون على حدة في البداية
+
+5
+00:00:22,170 --> 00:00:27,150
+نحكي مقدمة عن هذه الطريقة و كيف تم اكتشافهافي
+
+6
+00:00:27,150 --> 00:00:32,530
+عندنا عالم اسمه michael twist هاد عالم روسي استخدم
+
+7
+00:00:32,530 --> 00:00:37,070
+هذه التقنية لفصل أصباغ النباتات وورقة النباتات
+
+8
+00:00:37,070 --> 00:00:41,670
+تحتوي على عدة أنواع من الأصباغ قام في فصل هذه
+
+9
+00:00:41,670 --> 00:00:47,130
+الأصباغ بطريقة أطلق عليها اسم chromatography حكى
+
+10
+00:00:47,130 --> 00:00:50,030
+أن chromatography تعني written color
+
+11
+00:00:50,030 --> 00:00:54,730
+chromatography تعني written color معناها الكتابة
+
+12
+00:00:54,730 --> 00:01:01,040
+بالألوانلأن هو استخدم هذه الطريقة لفصل أصباق
+
+13
+00:01:01,040 --> 00:01:06,320
+النباتات فأطلق عليها إن هي الكتابة بالألوان إنها
+
+14
+00:01:06,320 --> 00:01:11,520
+تستخدم للمواد الملونة لأن هو فصل مواد ملونة فصل
+
+15
+00:01:11,520 --> 00:01:17,730
+أصباقيحكي لإن معظم المواد المحيطة فينا هي حوالينا
+
+16
+00:01:17,730 --> 00:01:22,030
+هي عبارة عن خليط من مادتين أو أكتر most material
+
+17
+00:01:22,030 --> 00:01:24,350
+in our surroundings are a mixture of two or more
+
+18
+00:01:24,350 --> 00:01:28,150
+compounds إن معظم المكونات في الهواء الجوي أو
+
+19
+00:01:28,150 --> 00:01:33,450
+حوالينا في أي مكان هي عبارة عن خليط هذا الخليط إما
+
+20
+00:01:33,450 --> 00:01:38,870
+بيكون homogeneous أو heterogeneousالخليط إما بيكون
+
+21
+00:01:38,870 --> 00:01:43,570
+خليط متجانس homogenous أو خليط غير متجانس
+
+22
+00:01:43,570 --> 00:01:47,990
+heterogeneous إيش يعني homogenous are uniform in
+
+23
+00:01:47,990 --> 00:01:52,110
+composition؟ homogenous معناه خليط متجانس إنه
+
+24
+00:01:52,110 --> 00:01:58,470
+عبارة عن عدة مكونات ولكن يظهر كمادة واحدةزي مثلا
+
+25
+00:01:58,470 --> 00:02:01,630
+الهواء الجو اللي حوالنا يحتوي على عدة أنواع من
+
+26
+00:02:01,630 --> 00:02:06,270
+الغازات لكن هل مبين كل غاز؟ لأ مبين إن هو مكوّن
+
+27
+00:02:06,270 --> 00:02:11,150
+واحد heterogeneous إن ال mixture are not uniform
+
+28
+00:02:11,150 --> 00:02:15,590
+إنه عندنا خليط من عدة مكونات لكن مبين إن هذا يحتوي
+
+29
+00:02:15,590 --> 00:02:20,900
+على أكثر من مادة زي مين؟زي الزيت مع الميه تخلط
+
+30
+00:02:20,900 --> 00:02:24,680
+الزيت مع الميه مبين وبيفصلوا لطبقتين فمبين ان هو
+
+31
+00:02:24,680 --> 00:02:28,400
+عبارة عن اكثر من مادة عبارة عن مادتين واضحين مش
+
+32
+00:02:28,400 --> 00:02:32,460
+متجانسينهي مثال مكتوب أن الـ air هو عبارة عن
+
+33
+00:02:32,460 --> 00:02:35,580
+homogeneous mixture أما ال oil في ال water لما
+
+34
+00:02:35,580 --> 00:02:39,580
+يكون زيت مع مي فهو heterogeneous غير متجانس بيحكي
+
+35
+00:02:39,580 --> 00:02:42,920
+لي سواء الخليط كان homogeneous أو heterogeneous
+
+36
+00:02:42,920 --> 00:02:47,560
+نقدر نفصله نعمل separated by physical method بطرق
+
+37
+00:02:47,560 --> 00:02:51,760
+فيزيائية عدة، إذا سواء الخليط كان عندى متجانس أو
+
+38
+00:02:51,760 --> 00:02:57,000
+غير متجانس بقدر أفصل مكوناته باستخدام عدة طرق في
+
+39
+00:02:57,000 --> 00:03:01,920
+طرق فيزيائيةكيف بدأ اختار الطريقة؟ احنا عندنا عدة
+
+40
+00:03:01,920 --> 00:03:05,120
+طرق للـ separation كيف بدأ اختار الطريقة المناسبة
+
+41
+00:03:05,120 --> 00:03:08,580
+لفصل الخليط بناء على الـtype of mixture على نوع
+
+42
+00:03:08,580 --> 00:03:11,160
+الخليط على الـdifference in the chemical
+
+43
+00:03:11,160 --> 00:03:15,760
+properties على الخصائص الكيميائية لالمكتشرمثلا أنا
+
+44
+00:03:15,760 --> 00:03:21,100
+هختار طريقة الفصل بناء على نوع الخليط أو بناء على
+
+45
+00:03:21,100 --> 00:03:25,340
+الخصائص الكيميائية يعني مثلا من طرق الفصل مثلا
+
+46
+00:03:25,340 --> 00:03:28,280
+عندنا الـ centrifugation الـ centrifugation يمكن
+
+47
+00:03:28,280 --> 00:03:33,110
+أخدته بالمقدمة أن استخدام جهاز centrifugeبيفصل
+
+48
+00:03:33,110 --> 00:03:38,170
+بناءً على الكثافة الدم مثلا نفصله باستخدام هذا
+
+49
+00:03:38,170 --> 00:03:42,310
+الجهاز باستخدام الـ centrifuge مثلا زي ما استخدمنا
+
+50
+00:03:42,310 --> 00:03:46,090
+isolation زي ما عزلنا الكازين من الملك هي هذه
+
+51
+00:03:46,090 --> 00:03:49,310
+طريقة فصل أنه أنا بس أخدت الكازين من كل مكونات
+
+52
+00:03:49,310 --> 00:03:54,150
+الحليب كانت الطريقة اسمها isolationمثلا عملية
+
+53
+00:03:54,150 --> 00:03:58,230
+precipitation أني أرسم مكون وأسيب الباقي وفي عندنا
+
+54
+00:03:58,230 --> 00:04:02,110
+تقنية chromatography وفي عندنا مثلا ال evaporation
+
+55
+00:04:02,110 --> 00:04:06,530
+التبخر أعمل تبخر زي مثلا مي مع ملح ببخر المي و بخل
+
+56
+00:04:06,530 --> 00:04:11,470
+الملح مثلا ال extraction استخلاص أعمل استخلاص
+
+57
+00:04:11,470 --> 00:04:15,570
+للمادة كل هدول طرق لالفصل اليوم احنا الطريقة اللي
+
+58
+00:04:15,570 --> 00:04:20,370
+بدنا ناخدها هي ال chromatographyبحكي للـ
+
+59
+00:04:20,370 --> 00:04:23,930
+chromatography مستخدمة بشكل واسع لفصل ال mixture
+
+60
+00:04:23,930 --> 00:04:29,050
+of compound into individual component لفصل الخليط
+
+61
+00:04:29,050 --> 00:04:33,890
+إلى مكوناته إنه بيخلي كل المادة لحال هذه التقنية
+
+62
+00:04:33,890 --> 00:04:38,630
+مستخدمة بشكل واسع لفصل الخليطاش معنى
+
+63
+00:04:38,630 --> 00:04:41,850
+chromatography؟ chromatography لأنه في البداية
+
+64
+00:04:41,850 --> 00:04:45,750
+استخدموها لفصل الألوان، لأصباغ النباتات، فأطلقوا
+
+65
+00:04:45,750 --> 00:04:50,350
+عليها أنها مستخدمة ل separation of color، لفصل
+
+66
+00:04:50,350 --> 00:04:54,130
+الألوان، ولكن في الوقت الحالي today ال
+
+67
+00:04:54,130 --> 00:04:57,680
+chromatography تعنيفصل ال color و ال colorless
+
+68
+00:04:57,680 --> 00:05:00,940
+substance إنها تستخدم للمواد الملونة و غير
+
+69
+00:05:00,940 --> 00:05:05,180
+الملونة، فإن هي قديما أول ما اكتشفت التقنية كانت
+
+70
+00:05:05,180 --> 00:05:09,240
+فقط بيعتقدوا إنها المواد الملونة، ولكن في اليوم
+
+71
+00:05:09,240 --> 00:05:14,420
+الحاضر هي المواد الملونة و غير الملونةالـ
+
+72
+00:05:14,420 --> 00:05:17,360
+chromatography هي طريقة فيزيائية physical method
+
+73
+00:05:17,360 --> 00:05:21,040
+for separation of mixture to its component
+
+74
+00:05:21,040 --> 00:05:26,820
+لمكوناته بناء على two face باستخدام two face طوران
+
+75
+00:05:26,820 --> 00:05:31,920
+حاجة بنسميها stationary face و حاجة بنسميها ال
+
+76
+00:05:31,920 --> 00:05:37,620
+mobile face إذن هي تقنية فيزيائية أو طريقة
+
+77
+00:05:37,620 --> 00:05:43,220
+فيزيائيةلفصل الخليط إلى مكوناته باستخدام two phase
+
+78
+00:05:43,220 --> 00:05:48,260
+ال mobile phase و ال stationary phase ايش هو ال
+
+79
+00:05:48,260 --> 00:05:51,380
+stationary phase ال stationary phase هو عبارة عن
+
+80
+00:05:51,380 --> 00:05:57,760
+الجزء الصلب بيكون solid مدعم ب glass بجزاز او
+
+81
+00:05:57,760 --> 00:06:02,400
+بتكون عبارة عن paper عن ورقة اذا ال stationary
+
+82
+00:06:02,400 --> 00:06:08,000
+phase هو الطور الساكن هو الطور الساكن بيكون عبارة
+
+83
+00:06:08,000 --> 00:06:12,960
+عنطبعا ورقة ممكن تكون صلبة هي ورقة صلبة تكون مدعمة
+
+84
+00:06:12,960 --> 00:06:17,540
+من الخلف بزجاج أو ألمانيون عشان تحميها من التكسر
+
+85
+00:06:17,540 --> 00:06:22,320
+وممكن تكون عبارة عن بابا خصائصها بتكون برس
+
+86
+00:06:22,320 --> 00:06:27,200
+material هي مادة مثقبة ال stationary phase هي مادة
+
+87
+00:06:27,200 --> 00:06:33,220
+مثقبة برس material ليش؟ عشان تسمح بدخول السائلThe
+
+88
+00:06:33,220 --> 00:06:36,400
+sample is carried by a particular solvent called
+
+89
+00:06:36,400 --> 00:06:40,080
+mobile phase كنت انا كيف تقنيتها دي بيكون في عندي
+
+90
+00:06:40,080 --> 00:06:44,380
+ورقة اسمها stationary phase هذا الطور الساكن هاي
+
+91
+00:06:44,380 --> 00:06:48,240
+بحط عليها العينات الخليط اللي انا بده افصله بحط
+
+92
+00:06:48,240 --> 00:06:50,860
+نقطة من الخليط اللي انا بفصله على ال stationary
+
+93
+00:06:50,860 --> 00:06:54,060
+phase على الطور الساكن في عندي حاجة اسمها ال
+
+94
+00:06:54,060 --> 00:06:57,680
+mobile phase ال mobile phase هو الطور المتحرك
+
+95
+00:06:57,680 --> 00:07:03,070
+بيكون عبارة عن غاز او liquid او ساعلوجدت الورقة
+
+96
+00:07:03,070 --> 00:07:05,750
+الـ stationary phase اللي أنا حطيت عليها الخليط
+
+97
+00:07:05,750 --> 00:07:11,900
+بقى حطها بغمرها في ال mobile phaseبغمر الورقة في
+
+98
+00:07:11,900 --> 00:07:14,840
+ال mobile phase بيبدأ ال mobile phase اللي هو
+
+99
+00:07:14,840 --> 00:07:18,940
+عبارة عن سائل أنه يمر عبر هذه الورقة زي مثلا ال
+
+100
+00:07:18,940 --> 00:07:22,460
+tissue paper لما تحطيها بمي بتتبلل بالمي بيمر المي
+
+101
+00:07:22,460 --> 00:07:25,860
+عبر ال tissue paper و هان هيك ال stationary phase
+
+102
+00:07:25,860 --> 00:07:28,420
+هي الورقة بيكون عليها الخليط أنا حطة نقطة من
+
+103
+00:07:28,420 --> 00:07:31,420
+الخليط عليها و بغمرها في ال mobile phase اللي هو
+
+104
+00:07:31,420 --> 00:07:35,870
+عبارة عن سائل بيمر السائل في هذه الورقةطول ما هو
+
+105
+00:07:35,870 --> 00:07:40,490
+بيمشي السائل بيفصل معاه الخليط لمكوناته على هيئة
+
+106
+00:07:40,490 --> 00:07:44,870
+نقاط كل .. كل مكوّن من هذه المكونات بيعطينا نقطة
+
+107
+00:07:44,870 --> 00:07:48,910
+في مكان معين فالـ Mobile phase هو عبارة عن الـ
+
+108
+00:07:48,910 --> 00:07:53,030
+solvent اسمه ممكن نحكي عنه الـ solvent أو الـluent
+
+109
+00:07:53,030 --> 00:07:58,170
+هو بيكون liquid أو gas الـ mobile phase نبقى
+
+110
+00:07:58,170 --> 00:08:02,770
+الستروزا stationary phaseالستشينري فيس هو جزء
+
+111
+00:08:02,770 --> 00:08:07,450
+الصلب بيكون عبارة عن ورقة ممكن و هي صلب بتكون
+
+112
+00:08:07,450 --> 00:08:11,830
+مدعمة بجلاس أو ألمنيوم ال mobile face عبارة عن
+
+113
+00:08:11,830 --> 00:08:16,030
+solvent اللي you want liquid أو غاز بيكون سائل أو
+
+114
+00:08:16,030 --> 00:08:21,730
+غازبغمر ال stationary phase في ال mobile phase
+
+115
+00:08:21,730 --> 00:08:24,850
+بحكي لي بعدين ال mobile phase بيمر عبر ال
+
+116
+00:08:24,850 --> 00:08:27,810
+stationary phase هيبدأ يمر داخل ال stationary
+
+117
+00:08:27,810 --> 00:08:31,910
+phase الورقة أنا لما أغمرها في السائل بيمشي السائل
+
+118
+00:08:31,910 --> 00:08:35,570
+فيها للأعلى فبيمر ال mobile phase عبر ال
+
+119
+00:08:35,570 --> 00:08:39,630
+stationary phaseطب في عندنا عدة مصطلحات، في عندنا
+
+120
+00:08:39,630 --> 00:08:43,830
+حاجة بنسميها affinity affinity هي المحبة معناها أو
+
+121
+00:08:43,830 --> 00:08:48,090
+التجاذب affinity تعني المحبة أو التجاذب هي
+
+122
+00:08:48,090 --> 00:08:51,030
+attraction or force between .. natural attraction
+
+123
+00:08:51,030 --> 00:08:54,330
+or force between two انسان هو التجاذب الطبيعي
+
+124
+00:08:54,330 --> 00:08:58,950
+للمواد مع بعضها البعض تجاذب الطبيعي للمواد مع
+
+125
+00:08:58,950 --> 00:09:04,030
+بعضها البعضمثلًا المي والسكة هاد بولر و هاد بولر
+
+126
+00:09:04,030 --> 00:09:08,270
+بيدوبوا ببعض يعني هما محببين لبعض فهما نفس الخصائص
+
+127
+00:09:08,270 --> 00:09:12,410
+فبيدوبوا ببعض لذن ال affinity هي التجاذب الطبيعي
+
+128
+00:09:12,410 --> 00:09:17,590
+للمواد مع بعضها البعض adsorption في أن adsorption
+
+129
+00:09:17,590 --> 00:09:22,090
+و absorption adsorption هي adhesion or stickness
+
+130
+00:09:22,090 --> 00:09:24,910
+of substance to the surface of another substance
+
+131
+00:09:24,910 --> 00:09:32,270
+والتصاق مادة على سطح مادة أخرىالتصاق مادة على سطح
+
+132
+00:09:32,270 --> 00:09:36,430
+مادة أخرى التصاق المادة بس على السطح بدون اختراق
+
+133
+00:09:36,430 --> 00:09:40,640
+التركيب الداخلي للمادة هاي ال absorptionabsorption
+
+134
+00:09:40,640 --> 00:09:47,000
+التصاق مادة على سطح مادة أخرى absorption معناها أن
+
+135
+00:09:47,000 --> 00:09:51,580
+المادة تخترق التركيب الداخلي للمادة الأخرى مش
+
+136
+00:09:51,580 --> 00:09:56,880
+التصاق فقط على السطح وإنما اختراق التركيب الداخلي
+
+137
+00:09:56,880 --> 00:10:02,020
+للمادة فيها نوعين رابط يوتيوب هاي بوضح الفرق بين
+
+138
+00:10:02,020 --> 00:10:06,660
+ال absorption و ال absorption إذا حبين تطلعوا عليه
+
+139
+00:10:08,390 --> 00:10:12,550
+هنا توضيح لتقنية ال chromatography نيجي أول شي على
+
+140
+00:10:12,550 --> 00:10:17,150
+اليسار في عندنا هنا الورقة البيضة هذه الورقة هي ال
+
+141
+00:10:17,150 --> 00:10:21,310
+stationary phase لذن الورقة هي الطور الساكن اللي
+
+142
+00:10:21,310 --> 00:10:24,590
+حكينا عنها انها stationary phase بتكون solid بتكون
+
+143
+00:10:24,590 --> 00:10:29,650
+مدعمهلجت؟ الخليط انا وان بده احطه .. المكونات ..
+
+144
+00:10:29,650 --> 00:10:32,690
+عندي خليط عندي كتيوب مليانة فيها خليط من المكونات
+
+145
+00:10:32,690 --> 00:10:36,210
+و بده افصلها باخد منها نقطة بحطها على هذه الورقة
+
+146
+00:10:36,210 --> 00:10:40,540
+على ال stationary phase في موقع مهينتمام؟ و بعدين
+
+147
+00:10:40,540 --> 00:10:44,460
+باخد هذه الورقة اللي صار عليها الخليط بغمرها في
+
+148
+00:10:44,460 --> 00:10:48,560
+داخل ال mobile face ال mobile face هو الجزء الأزرق
+
+149
+00:10:48,560 --> 00:10:51,160
+اللي تحت احنا هالجيت بالصورة اللي على الشمال
+
+150
+00:10:51,160 --> 00:10:54,440
+الورقة البيضة هي ال stationary face التور الساكن
+
+151
+00:10:54,440 --> 00:10:58,100
+حطيت عليها نقطة من هذا الخليط و الخليط كان عبارة
+
+152
+00:10:58,100 --> 00:11:02,820
+عن عدة مكونات هي تحت ملونة في منها أصفر و أحمر و
+
+153
+00:11:02,820 --> 00:11:05,440
+أزرق انها تحتوي على عدة مكونات
+
+154
+00:11:13,230 --> 00:11:17,560
+الجزء الأزرق اللي تحت هو عبارة عن liquid عن سائللا
+
+155
+00:11:17,560 --> 00:11:21,500
+جاتش بدي يسير بدي مر هذا السائل عبر الـ stationary
+
+156
+00:11:21,500 --> 00:11:25,100
+phase بيسير يمشي فيه عبر خاصية الـ capillary
+
+157
+00:11:25,100 --> 00:11:28,600
+action الخاصية الشعرية لأن ال stationary phase
+
+158
+00:11:28,600 --> 00:11:33,120
+حكينا أنها مادة مثقبة porous material فبيمر السائل
+
+159
+00:11:33,120 --> 00:11:37,180
+ال mobile phase عبرها وبيصعد للأعلى وبيظل يمشيطيب
+
+160
+00:11:37,180 --> 00:11:40,100
+كيف ده تفصل المواد طول ما هو بيمشي بيفصل الخليط
+
+161
+00:11:40,100 --> 00:11:44,200
+لعدد مكونات كل واحدة بتوجف في مكان بناء على إيش؟
+
+162
+00:11:44,200 --> 00:11:50,100
+بناء على الـ polarity وبناء على ال solubilityكيف؟
+
+163
+00:11:50,100 --> 00:11:52,440
+إن الورقة اللي أنا بستخدمها ال stationary face
+
+164
+00:11:52,440 --> 00:11:56,420
+بيكون إلها خصائص يعني ممكن تكون polar أو ممكن تكون
+
+165
+00:11:56,420 --> 00:11:59,800
+non polar على اعتبار مثلا ال stationary face هذه
+
+166
+00:11:59,800 --> 00:12:03,120
+اللي استخدمتها هي polar و ال mobile face برضه
+
+167
+00:12:03,120 --> 00:12:06,460
+بيكون إله خصائص إذا ال stationary face كان polar
+
+168
+00:12:06,460 --> 00:12:10,560
+إذا ال mobile face non polarتمام انا بدي اعتبر ال
+
+169
+00:12:10,560 --> 00:12:13,840
+stationary phase هنا ان هو polar و ال mobile phase
+
+170
+00:12:13,840 --> 00:12:17,420
+non polar اجت من اللي بيمشي ال mobile phase هو
+
+171
+00:12:17,420 --> 00:12:21,260
+اللي بيصعد ال stationary phase سابتة مدام ال
+
+172
+00:12:21,260 --> 00:12:25,500
+mobile phase اللي بيصعد هو non polar فالمواد اللي
+
+173
+00:12:25,500 --> 00:12:29,320
+في الخليط إذا كانت non polar هتمشي مع ال mobile
+
+174
+00:12:29,320 --> 00:12:34,740
+phase لفوق تمشي معاه إذا كانت polar هتقف لتحت عند
+
+175
+00:12:34,740 --> 00:12:37,700
+ال stationary phase هتظلها سابتة تحت ومش هتمشي
+
+176
+00:12:38,360 --> 00:12:41,680
+لأنها بتحب ال stationary fast هي خصائصها polar زي
+
+177
+00:12:41,680 --> 00:12:45,300
+ال stationary fast إذا هتوجه فتحت و تدلها مدزجة
+
+178
+00:12:45,300 --> 00:12:48,460
+فيه في ال stationary مش هتمشي مع ال mobile اللي
+
+179
+00:12:48,460 --> 00:12:52,500
+تكون خصائصها زي ال mobile fast مثلا non-polar زيه
+
+180
+00:12:52,500 --> 00:12:56,900
+بتمشي معاه للأعلىففي بعد الفاص اللي بيحكي لي هي
+
+181
+00:12:56,900 --> 00:12:59,560
+separation سهم و حطليها في الصورة التانية اللي على
+
+182
+00:12:59,560 --> 00:13:02,660
+اليمين ماشي ال mobile face السائل الأزرق و طلع
+
+183
+00:13:02,660 --> 00:13:07,700
+الورق لفوق و هو بيمشي بيفصل المكونات هالجت اللي
+
+184
+00:13:07,700 --> 00:13:11,860
+تحت ضاله المربعات أو المستطيلات الزرق هاي معناها
+
+185
+00:13:11,860 --> 00:13:14,980
+مدامها ضالت لتحت ملزقة إذا ان هي بتحب ال
+
+186
+00:13:14,980 --> 00:13:20,640
+stationary face هي محببة خصائصها نفس خصائص ال
+
+187
+00:13:20,640 --> 00:13:25,060
+stationary faceاللي بتضلها ملزقة تحت خصائصها نفس
+
+188
+00:13:25,060 --> 00:13:27,740
+خصائص ال stationary phase يعني إذا ال stationary
+
+189
+00:13:27,740 --> 00:13:31,680
+polar إذا هي polar زيه مابتحبش ال mobile فماطلعتش
+
+190
+00:13:31,680 --> 00:13:36,100
+معاه اللي بعدها بتكون أقل polarity اللي بعدها أقل
+
+191
+00:13:36,100 --> 00:13:40,480
+اللي بتطلع للآخر إذا هي نفس خصائص ال mobile phase
+
+192
+00:13:40,480 --> 00:13:46,010
+فطلعت معاه للآخريعني ال affinity المحبة للي تحت
+
+193
+00:13:46,010 --> 00:13:49,230
+دلت بنسجة تحت تكون ال affinity عالية مع ال
+
+194
+00:13:49,230 --> 00:13:52,790
+stationary face اللي فوق الأصفر الدوائر الأصفر
+
+195
+00:13:52,790 --> 00:13:56,050
+مدامها طلعت مع ال mobile face وطلعت للآخر إذا إن
+
+196
+00:13:56,050 --> 00:14:01,300
+هي ال affinity عالية مع ال mobile faceالـ
+
+197
+00:14:01,300 --> 00:14:04,340
+chromatography technique ايش عملتلي؟ عملتلي
+
+198
+00:14:04,340 --> 00:14:07,980
+analyze عملتلي تحليل حللتلي ال mixture لها عدة
+
+199
+00:14:07,980 --> 00:14:12,260
+مكونات عملتلي identify انه عرفتني هذا ال mixture
+
+200
+00:14:12,260 --> 00:14:18,260
+شو مكوناته purify عملتلي تنقية بدل ما كان خليط من
+
+201
+00:14:18,260 --> 00:14:23,930
+عدة مكونات صار كل مكون لحالعملت لي quantify كمي
+
+202
+00:14:23,930 --> 00:14:28,010
+أنه أنا عرفت هذا الخليط مكون من أكم من مادة يحتوي
+
+203
+00:14:28,010 --> 00:14:32,130
+على أكم من مادة يعني هو فصل لعدة مواد كل واحد في
+
+204
+00:14:32,130 --> 00:14:36,910
+منطقة مثلا الأصفر هي مادة أحمر مادتين ودوائر
+
+205
+00:14:36,910 --> 00:14:40,930
+السودة هي تلت مواد والمستطيلات هي أربع مواد إذا
+
+206
+00:14:40,930 --> 00:14:46,820
+هذا الخليط يتكون من أربع موادهنا التفصيل للي
+
+207
+00:14:46,820 --> 00:14:51,060
+توحكناه كله تقنية الـ separation is based on fact
+
+208
+00:14:51,060 --> 00:14:54,280
+that برص solid absorbed different substance to
+
+209
+00:14:54,280 --> 00:14:57,720
+different extreme depend on polarity أنه عندنا
+
+210
+00:14:57,720 --> 00:15:01,620
+مادة بتكون برص اللي هي عبارة عن stationary phase
+
+211
+00:15:02,220 --> 00:15:07,360
+تكون بورص material مادة مثقبة أنا هحط عليها القليل
+
+212
+00:15:07,360 --> 00:15:11,360
+اللي انا بده افصله وهيتم الفصل او انها تلتصق في
+
+213
+00:15:11,360 --> 00:15:14,280
+هذه ال stationary phase المكون اذا لتصق في ال
+
+214
+00:15:14,280 --> 00:15:17,320
+stationary phase او انه يمشي مع ال mobile phase
+
+215
+00:15:17,320 --> 00:15:21,440
+ومايلزجش بناء على ايش؟ بناء على ال polarity على
+
+216
+00:15:21,440 --> 00:15:25,200
+القطبية اذا ال stationary polar اذا المواد ال
+
+217
+00:15:25,200 --> 00:15:28,260
+polar هتضلهم لزجة فيه والمواد ال non polar هتطلع
+
+218
+00:15:28,260 --> 00:15:32,630
+لفوقإذا الـ stationary non-polar إذا المواد الـ
+
+219
+00:15:32,630 --> 00:15:38,060
+non-polar بتضالهم لزجة فيه و الـ polar بيطلع لفاهa
+
+220
+00:15:38,060 --> 00:15:40,980
+mixture to be separated is first applied to an
+
+221
+00:15:40,980 --> 00:15:44,440
+immovable برة solid إذا المكتشر الخليط اللي أنا
+
+222
+00:15:44,440 --> 00:15:47,540
+بده أفصله وين بحطه أول إشي بالبداية بحطه على ال
+
+223
+00:15:47,540 --> 00:15:51,080
+stationary ولا على ال mobile لأ بأسقطه في ال
+
+224
+00:15:51,080 --> 00:15:54,200
+stationary phase في البرة ال solid material اللي
+
+225
+00:15:54,200 --> 00:15:57,720
+هي المادة المثقبة اللي بتكون عبارة عن ورقة حكاية
+
+226
+00:15:57,720 --> 00:16:02,880
+أو ألمنيوم أو سليكا اللي هي اسمها stationary phase
+
+227
+00:16:03,510 --> 00:16:06,430
+the component of the mixture then get washed
+
+228
+00:16:06,430 --> 00:16:10,610
+وبعدين بغمره حكاية البر السايماتيريال اللي هي ال
+
+229
+00:16:10,610 --> 00:16:14,350
+stationary phase بغمرها في ال solvent اللي بنسميه
+
+230
+00:16:14,350 --> 00:16:18,990
+mobile phase بعدين ال mixture بيصيرله separated by
+
+231
+00:16:18,990 --> 00:16:22,830
+different بناء على إيش؟ بناء على ال attraction،
+
+232
+00:16:22,830 --> 00:16:26,850
+بناء على ال polarity مع ال stationary phase و ال
+
+233
+00:16:26,850 --> 00:16:30,970
+mobile phase، بناء على أي مبدأ، بناء على مبدأ
+
+234
+00:16:30,970 --> 00:16:34,960
+like، dissolve likeلذن بعد ما أحط المواد على ال
+
+235
+00:16:34,960 --> 00:16:38,620
+stationary phase بغمرها في ال mobile phase بيبدأ
+
+236
+00:16:38,620 --> 00:16:41,420
+يصعد ال mobile phase عبر ال stationary phase
+
+237
+00:16:41,420 --> 00:16:46,340
+وهيمشي معاه مواد هتمشي معاه مكونات الخليط بناء على
+
+238
+00:16:46,340 --> 00:16:50,220
+إيش، بناء على ال polarity، بناء على ال affinity،
+
+239
+00:16:50,220 --> 00:16:54,830
+بناء على ال attractionالتجادب بينها وبين الـ Face
+
+240
+00:16:54,830 --> 00:16:58,930
+إذا هي والله متحبة و Affinity وقوة تجادب عالية
+
+241
+00:16:58,930 --> 00:17:02,590
+وخصائصها الـ Polarity نفس خصائص الـ Stationary إذا
+
+242
+00:17:02,590 --> 00:17:05,970
+هتظلها ملزجة في الـ Stationary مش هتصعد للأعلى إذا
+
+243
+00:17:05,970 --> 00:17:09,690
+خصائصها و الـ Affinity و التجادب قوي مع الـ Mobile
+
+244
+00:17:09,690 --> 00:17:16,270
+Face إذا هي هتصعد مع الـ Mobile Face للأعلىif the
+
+245
+00:17:16,270 --> 00:17:19,330
+stationary phase is polar إذا الـ stationary كانت
+
+246
+00:17:19,330 --> 00:17:22,030
+polar إذا أن الـ polar component المكونات اللي
+
+247
+00:17:22,030 --> 00:17:27,740
+بتكون موجودة في الخليط كانت polarهي هتلزج في ال
+
+248
+00:17:27,740 --> 00:17:32,060
+stationary phase مش هتطلع لفوق و ال non-polar هي
+
+249
+00:17:32,060 --> 00:17:36,260
+اللي هتطلع أسرع للأعلى لكن لو ال stationary phase
+
+250
+00:17:36,260 --> 00:17:40,180
+is non-polar إذا ال non-polar هي اللي هتتجادب مع
+
+251
+00:17:40,180 --> 00:17:43,840
+ال stationary و تضلها تحت و ال polar هو اللي هيصعد
+
+252
+00:17:43,840 --> 00:17:50,860
+للأعلى بقوة أعلىأو بواجهة أقل الـ separation
+
+253
+00:17:50,860 --> 00:17:54,680
+depends on several factors عملية الفصل بتعتمد على
+
+254
+00:17:54,680 --> 00:17:59,640
+عدة عوامل عملية الفصل بتعتمد على عدة عوامل في
+
+255
+00:17:59,640 --> 00:18:03,620
+عندنا عاملين رئيسيين وفي عندنا التالت أول عامل هي
+
+256
+00:18:03,620 --> 00:18:10,060
+solubility solubility with mobile faceأول عامل الـ
+
+257
+00:18:10,060 --> 00:18:13,400
+solubility with mobile face اللي مكتوب هنا الـ
+
+258
+00:18:13,400 --> 00:18:16,460
+solvent الـ solvent معناه ال mobile face إذا كان
+
+259
+00:18:16,460 --> 00:18:19,780
+بيدوب في ال mobile face له نفس خصائص ال mobile
+
+260
+00:18:19,780 --> 00:18:24,520
+face إذا هو هيصعد للأعلى العامل التاني ال
+
+261
+00:18:24,520 --> 00:18:29,350
+attractionbetween compound and silica اللي هي قوة
+
+262
+00:18:29,350 --> 00:18:32,570
+التجاذب بين المادة اللي موجودة في الخليط والسيليكا
+
+263
+00:18:32,570 --> 00:18:35,450
+اللي هي ال stationary phase ال attraction يعني
+
+264
+00:18:35,450 --> 00:18:39,070
+التجاذب مع ال stationary phase إذا هي متجاذبة أو
+
+265
+00:18:39,070 --> 00:18:44,410
+حابة لل stationary إذا هتظلها في الأسفل إذا أول
+
+266
+00:18:44,410 --> 00:18:47,550
+عاملين رئيسيا يعتمد عليهم تقنية الفصل هي
+
+267
+00:18:47,550 --> 00:18:51,920
+solubility with mobile phaseوالـ Attraction with
+
+268
+00:18:51,920 --> 00:18:56,120
+stationary face وهذا هو نفسه الـ principle لما
+
+269
+00:18:56,120 --> 00:19:00,100
+أعمل اليوم أنه أنا بدي أعمل فصل لخريطة من مكونات
+
+270
+00:19:00,100 --> 00:19:03,920
+من عدة مكونات بناء على أيش بده يفصل، بناء على أي
+
+271
+00:19:03,920 --> 00:19:07,900
+مبدأ، بناء على مبدأ ال solubility with mobile face
+
+272
+00:19:07,900 --> 00:19:12,760
+أنه مدى الذائبية مع ال mobile face و ال attraction
+
+273
+00:19:12,760 --> 00:19:16,820
+مع الstationary phase ومدى التجاذب مع الـ
+
+274
+00:19:16,820 --> 00:19:19,380
+stationary phase والله إذا هي متجاذبة مع ال
+
+275
+00:19:19,380 --> 00:19:22,780
+stationary phase هتضلها لتحت ومش هتطلع إذا هي
+
+276
+00:19:22,780 --> 00:19:25,100
+solubility مع ال mobile phase اللي هي عالية
+
+277
+00:19:25,100 --> 00:19:29,280
+ويزائبة في ال mobile phase إذا هتصعد للأعلى تالت
+
+278
+00:19:29,280 --> 00:19:34,310
+عامل هو ال structure of compound ليه الحجم؟التالت
+
+279
+00:19:34,310 --> 00:19:38,570
+عامل يقصد فيه ال size حجم المادة اللى موجودة في
+
+280
+00:19:38,570 --> 00:19:42,930
+الخليط اللى بدي تمفصلها إذا حجمها كبير هتمشي بشواش
+
+281
+00:19:42,930 --> 00:19:47,830
+هتكون بطيقة في المشي وبالتالي مش هتمشي كتير هتكون
+
+282
+00:19:47,830 --> 00:19:53,330
+ملزقة لتحت أكتر لكن إذا كان حجمها صغير هتمشي أسرع
+
+283
+00:19:55,830 --> 00:20:00,770
+إيش مميزات ال chromatography ال advantage of
+
+284
+00:20:00,770 --> 00:20:04,650
+chromatography أول حاجة كان separate complex
+
+285
+00:20:04,650 --> 00:20:08,570
+mixture with greater precision أنها بتفصل خليط من
+
+286
+00:20:08,570 --> 00:20:14,490
+عدة مكونات بدقة عالية بتفصل خليط بدقة عالية يعني
+
+287
+00:20:14,490 --> 00:20:20,600
+لو في مادتين متشابهاتولكن بيختلفوا زي البروتين
+
+288
+00:20:20,600 --> 00:20:23,540
+مثلا هو عبارة عن سلسلة من أحمض أمينية لو في عندي
+
+289
+00:20:23,540 --> 00:20:27,320
+نوعين من البروتين هما نفس سلسلة أحمض الأمينية بس
+
+290
+00:20:27,320 --> 00:20:31,700
+بيختلفوا بحمض أميني واحد بتقدر تفصلوا هذه التقنية
+
+291
+00:20:31,700 --> 00:20:36,350
+تفصلهم عن بعضCan purify any soluble or volatile
+
+292
+00:20:36,350 --> 00:20:39,270
+substance if right adsorbent material, carrier
+
+293
+00:20:39,270 --> 00:20:42,930
+fluid and operating condition are employed بتقدر
+
+294
+00:20:42,930 --> 00:20:49,930
+تعملي تنقية لأي خليط من أي مواد، من عدة مواد تقدر
+
+295
+00:20:49,930 --> 00:20:53,930
+تنقي الخليط، أنه تخلي كل مكوّن لحال، بشرط أن
+
+296
+00:20:53,930 --> 00:20:57,630
+أستخدم ال stationary phase المناسب، ال mobile
+
+297
+00:20:57,630 --> 00:21:01,930
+phase المناسب والتقنيات و الخطوات إجراء التجربة
+
+298
+00:21:01,930 --> 00:21:07,280
+الصحيحةexact quantitative analysis is done even
+
+299
+00:21:07,280 --> 00:21:11,240
+from a trace compound بقدر أعمل منها quantitative
+
+300
+00:21:11,240 --> 00:21:16,560
+analysis اللي هو تحليل كمي أعرف كمية اربعة small
+
+301
+00:21:16,560 --> 00:21:19,780
+material consumption اللي هو هذه التقنية بتستهلكش
+
+302
+00:21:19,780 --> 00:21:24,920
+مواد كتيرة، مواد بسيطة ال quantization has a broad
+
+303
+00:21:24,920 --> 00:21:30,200
+linearity range الها مدى واسع، الها مدى واسع من
+
+304
+00:21:30,200 --> 00:21:35,680
+المواد اللي تقدر تفصلهاستة analyze of several
+
+305
+00:21:35,680 --> 00:21:39,720
+compounds can be done during one run بقدر أفصل
+
+306
+00:21:39,720 --> 00:21:43,280
+أكتر من خليط مرة واحدة يعني بجيب ال stationary
+
+307
+00:21:43,280 --> 00:21:47,100
+phase اللي بده أحط عليه الخليط بقى بحط خليط في
+
+308
+00:21:47,100 --> 00:21:50,580
+مكان بحط نقطة من هذا الخليط في مكان تاني نقطة من
+
+309
+00:21:50,580 --> 00:21:55,420
+الخليط التاني في نفس الورقة بقدر أفصل أدت خليط أدت
+
+310
+00:21:55,420 --> 00:21:59,840
+أنواع من الخليط ممكن خليط اتنين تلاتة في نفس ال
+
+311
+00:21:59,840 --> 00:22:05,130
+stationary phase في نفس التقنية في نفس المرةالـ
+
+312
+00:22:05,130 --> 00:22:07,930
+chromatography is a fast analysis method هي طريقة
+
+313
+00:22:07,930 --> 00:22:12,650
+سريعة و آخر إشي أنه حالياً صارت instrumentation
+
+314
+00:22:12,650 --> 00:22:15,910
+صارت عبارة عن أجهزة صارت automation بدل ما نعملها
+
+315
+00:22:15,910 --> 00:22:20,190
+بشكل يدوي اتم تقنية الفصل باستخدام أجهزة
+
+316
+00:22:22,690 --> 00:22:26,430
+في عندنا سؤال is this a pure substance or mixture؟
+
+317
+00:22:26,430 --> 00:22:31,270
+انه هل المادة اللي عندي هي مادة عبارة عن مادة نقية
+
+318
+00:22:31,270 --> 00:22:34,890
+بتتكون من مكون واحد ولا هي عبارة عن خليط كيف
+
+319
+00:22:34,890 --> 00:22:39,170
+بتعرف؟ انا أعطيتك مادة وحكيتلك اعرفيها هل هي pure
+
+320
+00:22:39,170 --> 00:22:43,090
+substance ولا mixture؟كيف بدك تعرفي؟ بيحكيلي
+
+321
+00:22:43,090 --> 00:22:46,830
+بنشتغل تقنية لـ chromatography بشكل عام، هذا ملخص
+
+322
+00:22:46,830 --> 00:22:49,670
+للـ slide هاي أن أنا بدي أشتغل تقنية لـ
+
+323
+00:22:49,670 --> 00:22:54,030
+chromatography هنكتب للـ TLC TLC هو نوع من أنواع
+
+324
+00:22:54,030 --> 00:22:58,790
+الكروماتوجرافي هنتعرف عليه لقدام هي اختصارها thin
+
+325
+00:22:58,790 --> 00:23:04,560
+layer chromatographyTLC Thin Layer Chromatography
+
+326
+00:23:04,560 --> 00:23:07,800
+بشكل عام انا كيف بده اعرف بده اجاوب عليها ده
+
+327
+00:23:07,800 --> 00:23:12,140
+السؤال هل هي pure مادة نقية ولا خليط بدي اشتغل
+
+328
+00:23:12,140 --> 00:23:16,820
+تقنية ال chromatographyوأشتغلها بعد ما يفصل الخليط
+
+329
+00:23:16,820 --> 00:23:19,520
+أو يمشي ال mobile phase عبر ال stationary phase
+
+330
+00:23:19,520 --> 00:23:24,300
+إذا أعطاني نقطة واحدة أنه في مكان واحد نقطة واحدة
+
+331
+00:23:24,300 --> 00:23:28,520
+إذا هذه pure substance مادة نقية لأنه ماعطانيهش
+
+332
+00:23:28,520 --> 00:23:33,380
+غير مكون واحد إذا أعطاني أكتر من نقطة في أكتر من
+
+333
+00:23:33,380 --> 00:23:38,610
+مكان فهذا معناه أنه كان mixture كان عبارةعن خليط
+
+334
+00:23:38,610 --> 00:23:43,370
+وفصل إلى عدة مكونات وبالتالي أنا بقدر أعرف ال
+
+335
+00:23:43,370 --> 00:23:47,030
+number of components in a mixture بقدر أعرف عدد
+
+336
+00:23:47,030 --> 00:23:50,850
+مكونات الخليط كيف بده يعرف عدد مكونات الخليط؟ لإنه
+
+337
+00:23:50,850 --> 00:23:55,290
+بعد ما يفصل أكم نقطة أعطاني مثلا إذا أعطاني تلاتة
+
+338
+00:23:55,290 --> 00:23:58,970
+إذا هو عبارة عن تلات مكونات إذا أعطاني بس نقطين
+
+339
+00:23:58,970 --> 00:24:04,420
+إذا هو عبارة عن مكوناتطيب are these compounds
+
+340
+00:24:04,420 --> 00:24:07,940
+identical؟ السؤال التاني انه بيحكيلي ان انا اعرف
+
+341
+00:24:07,940 --> 00:24:15,300
+هل هدول المواد identical شبه بعض انه عندك مادتين و
+
+342
+00:24:15,300 --> 00:24:19,840
+مش عارفين هل هم نفس المادة محطوطين في مكانين ولا
+
+343
+00:24:19,840 --> 00:24:23,700
+هم مادتين مختلفات كيف بدك تعرفي؟ برضه بيحكيلي
+
+344
+00:24:23,700 --> 00:24:26,900
+الإجابة اني بدي اشتغل ال chromatographic technique
+
+345
+00:24:26,900 --> 00:24:32,930
+بدي اشتغل تقنية ال chromatographyباخد من المادة
+
+346
+00:24:32,930 --> 00:24:35,750
+الأولى و بحطها على ال stationery face و المادة
+
+347
+00:24:35,750 --> 00:24:38,470
+التانية و بحطها برضه على الجانب التاني في نفس
+
+348
+00:24:38,470 --> 00:24:41,810
+الورقة كل واحدة في مكان و بحطها في ال mobile face
+
+349
+00:24:41,810 --> 00:24:45,730
+بغمرها و بيبدأ يفصل بحكي لي إذا فصلوا النقطتين
+
+350
+00:24:45,730 --> 00:24:50,130
+مقابل بعض في نفس المكان مقابل بعض إذا هم نفس
+
+351
+00:24:50,130 --> 00:24:55,230
+المادة إذا فصلوا كل واحدة في مكان إذا هم مادتين
+
+352
+00:24:55,230 --> 00:25:01,190
+مختلفةإذا أنا هشتغل التنتين و أعملهم فصل إذا تم
+
+353
+00:25:01,190 --> 00:25:08,390
+الفصل في نفس المكان إذا هو عبارة عن identical
+
+354
+00:25:08,390 --> 00:25:12,710
+substance أما إذا فصلوا كل واحدة في مكان بيكونوا
+
+355
+00:25:12,710 --> 00:25:17,990
+مواد مختلفة وكيف كمان بدي أعرف الزيادة؟ نحسب حاجة
+
+356
+00:25:18,110 --> 00:25:22,510
+الـ retention factor هنتعرف عليه لقدام لكن أنا
+
+357
+00:25:22,510 --> 00:25:25,970
+بشكل عام كيف بدأ أعرف هل هي identical ولا لأ نفس
+
+358
+00:25:25,970 --> 00:25:30,770
+المادة أشتغل تقنية ال chromatography إذا فصل
+
+359
+00:25:30,770 --> 00:25:36,050
+المكونين إجبال بعض في نفس المكان على الورقة مقابل
+
+360
+00:25:36,050 --> 00:25:40,170
+بعض إذا هم نفس المادة إذا كل واحدة في مكان إذا هم
+
+361
+00:25:40,170 --> 00:25:47,310
+مادتين مختلفةtype of chromatography أقسام لـ
+
+362
+00:25:47,310 --> 00:25:50,230
+chromatography لجهة ال chromatography مقسم إلى
+
+363
+00:25:50,230 --> 00:25:54,690
+قسمين بناء على ال stationary و بناء على ال mobile
+
+364
+00:25:54,690 --> 00:25:59,110
+phase ال chromatography مقسم إلى جزئين بناء على ال
+
+365
+00:25:59,110 --> 00:26:02,190
+stationary إليه تقسيمة و بناء على ال mobile إليه
+
+366
+00:26:02,190 --> 00:26:05,670
+تقسيمة بناء على ال mobile phase فيه نوعين إما
+
+367
+00:26:05,670 --> 00:26:09,250
+بستخدم ال mobile phase يكون gas، غاز أو بكون
+
+368
+00:26:09,250 --> 00:26:13,230
+liquidبناء على ال stationary face في منه column
+
+369
+00:26:13,230 --> 00:26:17,510
+على شكل عمود و في منه planner على شكل سطح مستوي
+
+370
+00:26:17,510 --> 00:26:21,130
+اذا بناء على ال stationary face في منه اتنين
+
+371
+00:26:21,130 --> 00:26:25,570
+column عمود او planner مستوي و ال planner في منه
+
+372
+00:26:25,570 --> 00:26:30,150
+thin layer chromatography و في منه paper
+
+373
+00:26:30,150 --> 00:26:36,110
+chromatographyهنا جدول بيوضح انه او انه ملخص هاي
+
+374
+00:26:36,110 --> 00:26:38,790
+ال slide المطلوب منها اصلا منها انه ال
+
+375
+00:26:38,790 --> 00:26:41,890
+chromatography تقسيم ال chromatography بناء على ال
+
+376
+00:26:41,890 --> 00:26:45,510
+mobile phase ان في عندنا liquid chromatography و
+
+377
+00:26:45,510 --> 00:26:49,610
+gas chromatography بس لذا تقسيم ال chromatography
+
+378
+00:26:49,610 --> 00:26:53,010
+بناء على ال mobile phase في منها liquid و في منها
+
+379
+00:26:53,010 --> 00:26:57,780
+gasالـ classification أما بناءً عن ال stationary
+
+380
+00:26:57,780 --> 00:27:00,740
+phase بناءً عن ال stationary phase حكينا في منها
+
+381
+00:27:00,740 --> 00:27:05,100
+planner مستوي و في منها column عمودي ال planner ال
+
+382
+00:27:05,100 --> 00:27:08,260
+chromatography بيحكي إنها separation technique
+
+383
+00:27:08,260 --> 00:27:11,920
+which ال stationary phase بيكون عبارة عن إبلان هي
+
+384
+00:27:11,920 --> 00:27:15,420
+تقنية فصلبيكون فيها الستاشينة اللي فيها سطور
+
+385
+00:27:15,420 --> 00:27:19,740
+التابت عبارة عن plan عبارة عن سطح مستول هذا سطح
+
+386
+00:27:19,740 --> 00:27:25,820
+المستول plan إما بيكون بابر ورقة أو بيكون جزيئات
+
+387
+00:27:25,820 --> 00:27:30,340
+صلبة زي السيليكا أو cellulose أو aluminium oxide
+
+388
+00:27:30,340 --> 00:27:35,720
+أو ion exchange resin ومدعمة من الخلف بجزاز أو
+
+389
+00:27:35,720 --> 00:27:41,560
+aluminiumإذا الـ plan chromatography planar
+
+390
+00:27:41,560 --> 00:27:44,960
+chromatography تقنية لفصل المواد بيكون فيها
+
+391
+00:27:44,960 --> 00:27:49,220
+الستشرينا رفاس عبارة عن plan عبارة عن سطح مستوي و
+
+392
+00:27:49,220 --> 00:27:52,720
+ال plan had ممكن يكون على شكلين ممكن يكون بابر و
+
+393
+00:27:52,720 --> 00:27:58,180
+ورقة الستشرينا رفاس عبارة عن بابر أو بيكون عبارة
+
+394
+00:27:58,180 --> 00:28:05,900
+عن جزء صلب مدعم بجزاز أو ألمنيومعشان بيكون الجزء
+
+395
+00:28:05,900 --> 00:28:10,660
+من الأمام بتكسر بسهولة، بيكون لين، فبيكون مدعم من
+
+396
+00:28:10,660 --> 00:28:15,040
+الخلف بجزء من الـ glass أو الالمنيوم فمنها نوعين
+
+397
+00:28:15,040 --> 00:28:18,760
+البلانار إما thin layer chromatography أو بابا الـ
+
+398
+00:28:18,760 --> 00:28:21,480
+thin layer هي اللي حكينا عنها اللي بتكون عبارة عن
+
+399
+00:28:21,480 --> 00:28:25,120
+.. لسه شينري فاس عبارة عن طبقة رفيعة، بتكون thin
+
+400
+00:28:25,120 --> 00:28:29,330
+layer، طبقة رفيعةفعشانها رفيعة بتكون سهلة التكسر
+
+401
+00:28:29,330 --> 00:28:34,650
+فهي مدعمة سبارتت بجلاز plastic أو aluminium بجزاز
+
+402
+00:28:34,650 --> 00:28:38,690
+أو plastic أو aluminium ال paper chromatography
+
+403
+00:28:38,690 --> 00:28:41,930
+paper chromatography ال stationary face بيكون
+
+404
+00:28:41,930 --> 00:28:45,570
+عبارة عن paper عبارة عن ورقة أما الجزء التاني هو
+
+405
+00:28:45,570 --> 00:28:49,310
+ال column chromatography اللي بيكون عبارة عن عمود
+
+406
+00:28:49,310 --> 00:28:54,860
+فال stationary face بيكون columnطيب ليش بنستخدم
+
+407
+00:28:54,860 --> 00:28:57,480
+تقنية ال chromatography؟ بيحكينا ال
+
+408
+00:28:57,480 --> 00:29:00,580
+chromatographic technique ممكن نستخدمها ك
+
+409
+00:29:00,580 --> 00:29:06,820
+qualitative و ك quantitative ممكن استخدمها ك
+
+410
+00:29:06,820 --> 00:29:11,480
+qualitative و quantitative ايش يعني qualitative؟
+
+411
+00:29:11,480 --> 00:29:14,740
+يعني اعرف نوع المادة، اعرف اسم المادة، مين هذه
+
+412
+00:29:14,740 --> 00:29:20,370
+المادةQualitative معناها جودة أو نوع المادة تحديد
+
+413
+00:29:20,370 --> 00:29:24,610
+نوع المادة كيبدا حدد نوع المادة بناء على حاجة
+
+414
+00:29:24,610 --> 00:29:29,450
+اسمها ال retention factor انه انا بحط خليط من
+
+415
+00:29:29,450 --> 00:29:32,990
+المواد على ال stationary face و بحطها بغمرها بال
+
+416
+00:29:32,990 --> 00:29:37,650
+mobile face و بيبدأ يفصل بيفصل في كل مكون إلى نقاط
+
+417
+00:29:37,650 --> 00:29:43,170
+كل نقطة في مكان اجيت وين وصلت هذه النقطة؟من عندها
+
+418
+00:29:43,170 --> 00:29:47,670
+بحسب حاجة لكل نقطة بحسب إيش اسمه retention factor
+
+419
+00:29:48,850 --> 00:29:53,310
+اللي هو عامل المحافظة كيف بيانحسب هاد؟ هو موجود في
+
+420
+00:29:53,310 --> 00:29:56,410
+آخر slide بس أنه بشكل عام ال retention factor هو
+
+421
+00:29:56,410 --> 00:30:00,290
+عبارة عن ال distance اللي هي المسافة اللي قطعتها
+
+422
+00:30:00,290 --> 00:30:04,470
+المادة، بكون أنا حاطة خط بداية اللي هو حاطيت من
+
+423
+00:30:04,470 --> 00:30:07,190
+عنده الخليط، النقطة وين أنت حاطيت الخليط، من وين
+
+424
+00:30:07,190 --> 00:30:10,970
+بدأتي، من هنا أنا حاطيت النقطة، بحسب المسافة اللي
+
+425
+00:30:10,970 --> 00:30:13,930
+قطعتها المادة من هذا الخط لوين وصلت بعد الفصل
+
+426
+00:30:14,870 --> 00:30:19,730
+المادة وين وصلت بعد الفصل يعني لو رجعنا لهذه
+
+427
+00:30:19,730 --> 00:30:25,790
+الصورة هل جت المواد بعد الفصل مثلا الحمرة انت حطيت
+
+428
+00:30:25,790 --> 00:30:29,350
+الخليط وين؟ من تحت من خط البداية من تحت صح؟ بدي
+
+429
+00:30:29,350 --> 00:30:32,330
+أحسب المسافة من خط البداية اللي عند الحمرة بدي
+
+430
+00:30:32,330 --> 00:30:37,580
+أجيس بالمسطرة جدش قطعت الحمرة مسافةتمام؟ على
+
+431
+00:30:37,580 --> 00:30:41,640
+المسافة اللى قطحها الـ mobile phase من البداية
+
+432
+00:30:41,640 --> 00:30:45,300
+لوين وصل يعني الجزء الأزرق السائل الأزرق هاد من
+
+433
+00:30:45,300 --> 00:30:50,160
+وين كان من البداية لفوق لجزء الأزرق لآخر وين وصل
+
+434
+00:30:50,160 --> 00:30:53,220
+هذه المسافة كلها برضه بده جثة في المسطرة ال
+
+435
+00:30:53,220 --> 00:30:56,340
+retention factor هو عبارة عن المسافة اللى قطعتها
+
+436
+00:30:56,340 --> 00:31:00,760
+المادة من خط البداية على المسافة اللى قطحها ال
+
+437
+00:31:00,760 --> 00:31:05,370
+solvent كامل من خط البداية لعند ما وصلهذه اسمها
+
+438
+00:31:05,370 --> 00:31:09,010
+retention factor إذاً ال retention factor هي
+
+439
+00:31:09,010 --> 00:31:15,850
+المسافة اللي قطعتها المادة أعلى المسافة اللي قطعها
+
+440
+00:31:15,850 --> 00:31:19,190
+ال solvent من خط البداية إلى وين وصل كده بيطلع
+
+441
+00:31:19,190 --> 00:31:23,270
+عندي رقم كل مادة إلها retention factor معروف
+
+442
+00:31:23,270 --> 00:31:27,350
+ومحفوظ في جدول وجدول علماء ومكتوبة في جدول برجع
+
+443
+00:31:27,350 --> 00:31:31,250
+لهذا الجدول بدور على الرقم اللي طلع معاهretention
+
+444
+00:31:31,250 --> 00:31:34,970
+factor اللي طلعت معاه بطلع بروح بدور في الجدول
+
+445
+00:31:34,970 --> 00:31:39,050
+مثلا ستة بروح أشوف قبل الستة مين هي المادة بس بطلع
+
+446
+00:31:39,050 --> 00:31:43,730
+أسمها و بكون عارفيتها، بعرفها مثلا نص، بروح للجدول
+
+447
+00:31:43,730 --> 00:31:47,810
+بطلع مين النص؟ مثلا لل glycine طلعت النص، إذا
+
+448
+00:31:47,810 --> 00:31:51,390
+المادة هي عبارة عن glycine ف qualitative analysis
+
+449
+00:31:51,390 --> 00:31:54,950
+كيف؟ من خلال أني أحسب ال retention factor
+
+450
+00:31:56,250 --> 00:31:59,370
+Quantitative determination برضه بيستخدم ك
+
+451
+00:31:59,370 --> 00:32:04,050
+quantitative quantitative يعني كمية يعني بتعرف
+
+452
+00:32:04,050 --> 00:32:08,390
+كمية المادة الموجودة جداش كميتها بيحكيلي هذا بيتم
+
+453
+00:32:08,390 --> 00:32:12,590
+معرفته بناء على ال intensity of a spot وبناء على
+
+454
+00:32:12,590 --> 00:32:17,110
+ال side of the spot بناء على شدة ال only spot هي
+
+455
+00:32:17,110 --> 00:32:20,110
+اللي جيت بعد ما يفصل المواد وبيعطينيها على شكل
+
+456
+00:32:20,110 --> 00:32:25,010
+نقاط spot بنسميها نقاطهذه النقطة جدّيش حجمها
+
+457
+00:32:25,010 --> 00:32:29,050
+الدائرة اللي طلعت معي جدّيش حجمها اللي هي صغيرة
+
+458
+00:32:29,050 --> 00:32:33,750
+ولا كبيرة طب الشد تعدها واضحة، نغامجة ولا إنها
+
+459
+00:32:33,750 --> 00:32:38,710
+خفيفة؟ هذه بتحدد لكمية المادة إذا كان مثلا حجمها
+
+460
+00:32:38,710 --> 00:32:43,110
+كبير إذا هي مادة موجودة بكمية كبيرة إذا حجمها صغير
+
+461
+00:32:43,110 --> 00:32:47,570
+موجودة بكمية أقل تمام، بدي أعرف بزيادة ال
+
+462
+00:32:47,570 --> 00:32:51,010
+quantitative analysis بيستخدموا جهاز اسمه الـ
+
+463
+00:32:51,010 --> 00:32:57,770
+densitometerجهاز اسمه densitometer يستخدم في تقنية
+
+464
+00:32:57,770 --> 00:33:02,090
+ال chromatography for quantitative determination
+
+465
+00:33:02,090 --> 00:33:07,490
+يعني هو جهاز instrument to detect the color .. the
+
+466
+00:33:07,490 --> 00:33:12,330
+intensity of color هو جهاز يستخدم لمقياس شدة لون
+
+467
+00:33:12,330 --> 00:33:15,810
+لثبوت اللي ظهر عندي في تقنية ال chromatography
+
+468
+00:33:15,810 --> 00:33:21,750
+عشان يعطيني quantitative determination لالمادةإذا
+
+469
+00:33:21,750 --> 00:33:25,490
+بيستخدم التقنية الـ chromatography for qualitative
+
+470
+00:33:25,490 --> 00:33:33,150
+و quantitative للكمي و الجودةpaper chromatography
+
+471
+00:33:33,150 --> 00:33:36,450
+ال paper احنا اليوم هنستخدم paper chromatography
+
+472
+00:33:36,450 --> 00:33:39,430
+ال paper chromatography حكينا ان ال stationary
+
+473
+00:33:39,430 --> 00:33:43,230
+phase عبارة عن paper و ال solvent ال mobile phase
+
+474
+00:33:43,230 --> 00:33:47,410
+بيكون ليكوين ال paper chromatography بحط عليها
+
+475
+00:33:47,410 --> 00:33:50,670
+العينات بحمل عليها العينات ان انا بدي احط الخليط
+
+476
+00:33:50,670 --> 00:33:53,930
+من المواد على ال paper على ال stationary phase على
+
+477
+00:33:53,930 --> 00:33:57,430
+هيئة ال spot بحط نقطة من هذا الخليط على هذه الورقة
+
+478
+00:33:57,970 --> 00:34:01,410
+بعدين الورقة بغمرها في ال solvent بغمرها dipped
+
+479
+00:34:01,410 --> 00:34:06,130
+into a solvent بغمرها في ال mobile phase بيبدأ
+
+480
+00:34:06,130 --> 00:34:09,410
+يطلع يمشي ال mobile phase عبر ال stationary phase
+
+481
+00:34:09,410 --> 00:34:13,570
+بيمشي السائل للأعلى بناء على خاصية ال capillary
+
+482
+00:34:13,570 --> 00:34:19,050
+action الخاصية الشعرية و طول ما هو ماشي السائل
+
+483
+00:34:19,050 --> 00:34:22,770
+بتنفصل الخليط إلى مكوناته بناء على إيش حكينا
+
+484
+00:34:22,770 --> 00:34:26,950
+الفصل؟ بناء على ال solubility و ال attractionمدى
+
+485
+00:34:26,950 --> 00:34:31,050
+ذائبيتها في الـ Mobile Face ومدى تجاذبها مع الـ
+
+486
+00:34:31,050 --> 00:34:36,450
+Stationary Face إذا أنا ده تكرار الكلام كلهالـ
+
+487
+00:34:36,450 --> 00:34:39,790
+principle of paper chromatography الـ capillary
+
+488
+00:34:39,790 --> 00:34:42,770
+action هى شىه هى movement of liquid within the
+
+489
+00:34:42,770 --> 00:34:47,490
+space of a porous material هى حركة ال mobile phase
+
+490
+00:34:47,490 --> 00:34:50,710
+عبر ال stationary phase ال capillary action هى
+
+491
+00:34:50,710 --> 00:34:53,410
+عبارة عن movement of liquid اللى هو ال mobile
+
+492
+00:34:53,410 --> 00:34:56,570
+phase within a porous material اللى هى ال
+
+493
+00:34:56,570 --> 00:35:00,430
+stationary phase بس بتعبير آخر هى حركة ال mobile
+
+494
+00:35:00,430 --> 00:35:04,890
+phase عبر ال stationary phaseالـ Liquid اللي هو
+
+495
+00:35:04,890 --> 00:35:08,230
+الـ mobile phase بيتحرك عبر ال paper بسبب أن
+
+496
+00:35:08,230 --> 00:35:12,850
+الجاذبية ال attraction التجاذب أقوى من قوة
+
+497
+00:35:12,850 --> 00:35:18,860
+الجاذبيةبناء على أي مبدأ هيتم الفصل وبناء على إيش
+
+498
+00:35:18,860 --> 00:35:22,760
+حكينا بناء على like dissolve like وبناء على ال
+
+499
+00:35:22,760 --> 00:35:26,900
+solubility مع ال mobile phase و ال affinity أو ال
+
+500
+00:35:26,900 --> 00:35:30,940
+attraction with stationary phase إذا ال principle
+
+501
+00:35:30,940 --> 00:35:36,080
+لو بدي ألخصه هو حاجتين ال affinity with mobile ال
+
+502
+00:35:36,080 --> 00:35:39,740
+affinity أو attraction with stationary phase و
+
+503
+00:35:39,740 --> 00:35:45,480
+solubility with mobile phaseالـ Thin Layer
+
+504
+00:35:45,480 --> 00:35:51,280
+Chromatography هي تقنية لفصل المواد بيكون فيها الـ
+
+505
+00:35:51,280 --> 00:35:55,260
+stationary phase عبارة عن Thin Layer عبارة عن طبقة
+
+506
+00:35:55,260 --> 00:35:59,940
+خفيفة رفيعة of silica بتكون عبارة عن هذا الطبقة
+
+507
+00:35:59,940 --> 00:36:05,300
+سيليكا أو ألمنيوم سيليكا مدعمة بجزاز أو ألمنيوم
+
+508
+00:36:05,300 --> 00:36:09,950
+برضه لتدعيمإذا الـ thin layer chromatography هي
+
+509
+00:36:09,950 --> 00:36:13,350
+عبارة عن سيليكا بيكون ال stationary phase ورقة
+
+510
+00:36:13,350 --> 00:36:18,870
+رفية من السيليكا مدعمة بجزاز أو ألمنيوم السيليكا
+
+511
+00:36:18,870 --> 00:36:22,350
+جيل هتكون هي ال stationary phase أما ال solvent هو
+
+512
+00:36:22,350 --> 00:36:25,650
+ال mobile phase إذا ال thin layer chromatography
+
+513
+00:36:25,650 --> 00:36:29,770
+بدك تكون عارفة إن هي عبارة عن سيليكا ال stationary
+
+514
+00:36:29,770 --> 00:36:33,770
+phase عبارة عن سيليكا و ال solvent هو ال mobile
+
+515
+00:36:33,770 --> 00:36:40,250
+phaseإيش مبدأ الفصل ال principle ملخص ال principle
+
+516
+00:36:40,250 --> 00:36:44,670
+كله حكيناه إنه عبارة عن affinity with stationary
+
+517
+00:36:44,670 --> 00:36:50,570
+phase و attraction with mobile with stationary
+
+518
+00:36:50,570 --> 00:36:53,650
+phase و solubility with mobile phase لجيت هذا
+
+519
+00:36:53,650 --> 00:36:57,030
+الكلام اللي هجيت مكتوب في ال principle كله تكرار
+
+520
+00:36:57,030 --> 00:37:00,530
+للي قلناها different compound in sample mixture
+
+521
+00:37:00,530 --> 00:37:03,030
+travel different distance according to how
+
+522
+00:37:03,030 --> 00:37:06,470
+stronglythey interact with the stationary phase
+
+523
+00:37:06,470 --> 00:37:10,370
+and mobile phase بيحكيلي المواد بتمشي اللي موجودة
+
+524
+00:37:10,370 --> 00:37:14,570
+في الخلط بتنفصلها و بتمشي مسافات مختلفة كل واحدة
+
+525
+00:37:14,570 --> 00:37:18,750
+بتقف في مكان في مسافة مختلفة في مكان مختلف بناء
+
+526
+00:37:18,750 --> 00:37:21,910
+على ال attraction بناء على ال interact بناء على
+
+527
+00:37:21,910 --> 00:37:25,770
+التجادب مع ال stationary phase و ال mobile phase
+
+528
+00:37:31,430 --> 00:37:37,310
+المواد اللي موجودة في الخليط بتختلف في قوة ذائبية
+
+529
+00:37:37,310 --> 00:37:41,470
+والالتصاق مع ال stationary أو مع ال mobile phase
+
+530
+00:37:41,470 --> 00:37:44,850
+ال mobile phase اللي هو ال liquid أو ال eloquent
+
+531
+00:37:44,850 --> 00:37:46,110
+نفس مسمى
+
+532
+00:37:49,820 --> 00:37:53,080
+وهو يرتفع ببطء بيقول إن السائل اللي هو الـ mobile
+
+533
+00:37:53,080 --> 00:37:57,280
+phase بيمشي عبر الـ stationary phase اللي موجود في
+
+534
+00:37:57,280 --> 00:38:00,580
+الـ thin layer كروماتجرافي بالخاصية الشعرية بالـ
+
+535
+00:38:00,580 --> 00:38:04,680
+capillary action separation of compound is based
+
+536
+00:38:04,680 --> 00:38:07,940
+on the competition of the solute and the mobile
+
+537
+00:38:07,940 --> 00:38:11,740
+phase for binding substances بيقول لي إن فصل
+
+538
+00:38:11,740 --> 00:38:15,860
+المواد بناء على التنافس بين ال mobile phase و ال
+
+539
+00:38:15,860 --> 00:38:19,680
+stationary phase على هذه الموادمين ياخد مواد أكتر
+
+540
+00:38:19,680 --> 00:38:24,180
+وهذا بناء على solubility انا جيت اللي احنا
+
+541
+00:38:24,180 --> 00:38:27,840
+بنستخدمها هي thin layer chromatography و الـ thin
+
+542
+00:38:27,840 --> 00:38:33,040
+layer حكينا انه عبارة عن silica gel و السيليكا هي
+
+543
+00:38:33,040 --> 00:38:36,260
+polar فبيحكي لي if normal phase اذا احنا استخدمنا
+
+544
+00:38:36,260 --> 00:38:40,040
+silica gel as stationary phase ال stationary phase
+
+545
+00:38:40,040 --> 00:38:43,880
+يكون عندنا silica gel فهو polar فانا لو حطيت
+
+546
+00:38:43,880 --> 00:38:48,160
+مادتين بيختلفوا في ال polarity تمام؟المادة اللي
+
+547
+00:38:48,160 --> 00:38:51,620
+هتكون Polar، More Polar، Stronger Attraction
+
+548
+00:38:51,620 --> 00:38:55,740
+والسيلكا، مدام خصائصها Polar، المادة هي Polar، إذا
+
+549
+00:38:55,740 --> 00:38:59,080
+ان بتشبه خصائص السيلكا، هي Polar زيها، إذا انها
+
+550
+00:38:59,080 --> 00:39:02,800
+تلزج في الـstationary phase في السيلكا ومش هتمشي
+
+551
+00:39:02,800 --> 00:39:07,160
+للأعلى أما المواد الـNon Polar هي اللي هتمشي
+
+552
+00:39:07,160 --> 00:39:11,890
+لأعلى، move higherup to the plate فهتمشي أعلى
+
+553
+00:39:11,890 --> 00:39:16,850
+الأسرع للأعلى مع ال mobile phase لأن خصائصها تشبه
+
+554
+00:39:16,850 --> 00:39:20,270
+خصائص ال mobile هي non polar زي ال mobile لأن ال
+
+555
+00:39:20,270 --> 00:39:25,850
+stationary silica وهي polar فهذا
+
+556
+00:39:25,850 --> 00:39:29,530
+ال principle كله كلام زي اللي حكيناه ملخص ال
+
+557
+00:39:29,530 --> 00:39:34,490
+principle كله بكلمتين هي عبارة عن attraction with
+
+558
+00:39:34,490 --> 00:39:38,610
+stationary phase و solubility with mobile phase
+
+559
+00:39:40,250 --> 00:39:43,730
+retention factor حكينا عنه هو العامل المحافظة
+
+560
+00:39:43,730 --> 00:39:48,390
+بيحكي إنه كل مادة كيميائية لها retention factor
+
+561
+00:39:48,390 --> 00:39:54,830
+خاص فيها من خلالها بعرف هذه المادة كل مادة لها
+
+562
+00:39:54,830 --> 00:39:58,570
+retention factor خاص فيها أنا بحسب retention
+
+563
+00:39:58,570 --> 00:40:02,330
+factor بعد ما تفصل المواد بحسب لكل مادة retention
+
+564
+00:40:02,330 --> 00:40:06,520
+factorحكينا قانون ال retention factor إيش هو هو
+
+565
+00:40:06,520 --> 00:40:10,940
+المسافة اللي قطعتها المادة من البداية لون وصلت على
+
+566
+00:40:10,940 --> 00:40:14,280
+المسافة اللي قطعها ال solvent ال mobile phase من
+
+567
+00:40:14,280 --> 00:40:19,160
+البداية لون وصلت زي ما أنتوا شايفين هنا مثلا أجد
+
+568
+00:40:19,160 --> 00:40:21,980
+خط البداية اللي هو تحت أنا حاطيت الخليط من الخط
+
+569
+00:40:21,980 --> 00:40:26,200
+اللي تحت النقطة الحمراء مثلا بجيس المسافة بالمسطرة
+
+570
+00:40:26,200 --> 00:40:30,320
+من البداية لون هي وصلت على المسافة اللي مشيها
+
+571
+00:40:30,320 --> 00:40:35,520
+المحلو من خط البداية للنهايةوبشوفها بجسم على بعض
+
+572
+00:40:35,520 --> 00:40:39,000
+هذا الناتج وبيطلع عندي هذا ال retention factor
+
+573
+00:40:39,000 --> 00:40:47,020
+برجع للجدول المعروف و بشوف جداش لكل مادة the
+
+574
+00:40:47,020 --> 00:40:49,680
+stationary phase in thin layer chromatography نيجي
+
+575
+00:40:49,680 --> 00:40:54,080
+نحكي عن ال thin layer chromatographyالـ Thin Layer
+
+576
+00:40:54,080 --> 00:40:56,600
+Chromatography حكينا إن الـ Stachyna Rephase هي
+
+577
+00:40:56,600 --> 00:41:00,220
+عبارة عن Silica Gel و Silica Gel عبارة عن Polar
+
+578
+00:41:00,220 --> 00:41:03,860
+نيجي نتعرف على Silica Gel بيحكي إن Silica Gel is a
+
+579
+00:41:03,860 --> 00:41:06,000
+form of Silicon Dioxide هي عبارة عن Silicon
+
+580
+00:41:06,000 --> 00:41:10,900
+Dioxide بتكون من Silicon أتم من ذرات السيليكون
+
+581
+00:41:10,900 --> 00:41:15,560
+مرتبطة بجزيئات أكسجين ال structure الموجود فوق في
+
+582
+00:41:15,560 --> 00:41:19,220
+أقصى اليمين في الصورة من الأعلى هي عبارة عن ذرات
+
+583
+00:41:19,220 --> 00:41:25,100
+السيليكون الأسئعي مرتبطة مع الأكسجينوعلى السطح في
+
+584
+00:41:25,100 --> 00:41:29,540
+OH group attached to it إذا إن هي سيليكون ذرات من
+
+585
+00:41:29,540 --> 00:41:33,600
+السيليكون وموجود مرتبط معها أكسجين وعلى السطح في
+
+586
+00:41:33,600 --> 00:41:38,260
+OH الـ OH هي اللي خلتها بولا ال surface of silica
+
+587
+00:41:38,260 --> 00:41:43,340
+is بولا السطح عبارة عن OH فهو بولا can form
+
+588
+00:41:43,340 --> 00:41:48,530
+hydrogen bond ويقدر يعمل رابطة هيدروجينيةبما أنه
+
+589
+00:41:48,530 --> 00:41:52,790
+سطحها بولر إذا هو عبارة عن بولر وهي بيحكي اللي
+
+590
+00:41:52,790 --> 00:41:57,550
+بتكون مدعمة بألمنيوم أو جزاز وممكن تكون أصلا مش أي
+
+591
+00:41:57,550 --> 00:42:00,310
+نتس اللي كيكون نوع آخر لل stationary phase ممكن
+
+592
+00:42:00,310 --> 00:42:04,130
+يكون aluminium oxide يكون عبارة عن ألمنيوم مع
+
+593
+00:42:04,130 --> 00:42:09,520
+أكسوجين والسطح برضه قوات فهيكون بولر زيهابشكل عام
+
+594
+00:42:09,520 --> 00:42:11,720
+إن الـ stationary phase في الـ thin layer
+
+595
+00:42:11,720 --> 00:42:16,260
+كروماتجرافي عبارة عن سيليكون عبارة عن سيليكا جيل و
+
+596
+00:42:16,260 --> 00:42:21,000
+السيليكا على سطحها OH فالـ OH يعني polar فالـ thin
+
+597
+00:42:21,000 --> 00:42:25,520
+layer كروماتجرافي لو حكيتلك استخدام التقنية
+
+598
+00:42:25,520 --> 00:42:30,180
+الكروماتجرافي الـ thin layer كروماتجرافي بدك تفهمي
+
+599
+00:42:30,180 --> 00:42:34,060
+لحالك إن الـ stationary phase polar و ال mobile
+
+600
+00:42:34,060 --> 00:42:38,400
+phase non polarلو جبتلك هذا السؤال مثلا انه عندك
+
+601
+00:42:38,400 --> 00:42:41,380
+stationary phase عندك 10 layer chromatography و
+
+602
+00:42:41,380 --> 00:42:45,700
+عندك مادة A و مادة B A كانت polar و B non polar
+
+603
+00:42:45,700 --> 00:42:50,420
+مين اللي هتمشي إلى الفقر الأعلى أكتر؟ اذا انا بدي
+
+604
+00:42:50,420 --> 00:42:53,080
+افهم لحالي 10 layer chromatography إذا ال
+
+605
+00:42:53,080 --> 00:42:57,420
+stationary phase بدي يكون polar فالمادة A إذا كانت
+
+606
+00:42:57,420 --> 00:43:03,300
+هي ال polar هي اللي هتمشيإذا المادة A هي ال bowler
+
+607
+00:43:03,300 --> 00:43:07,640
+إذا ان هي مش هتمشي هي اللي هتضلها لتحت بس إذا
+
+608
+00:43:07,640 --> 00:43:12,600
+المادة B ال non bowler هي اللي هتمشي لفوق لأنه
+
+609
+00:43:12,600 --> 00:43:15,060
+thin layer chromatography بتدافع مع طول ال
+
+610
+00:43:15,060 --> 00:43:19,020
+stationary phase بيكون bowler فالمادة ال bowler
+
+611
+00:43:19,020 --> 00:43:22,020
+هتلزج و مش هتمشي للأعلى و المادة ال non bowler هي
+
+612
+00:43:22,020 --> 00:43:27,720
+اللي هتمشي للأعلىطب في عندنا تقنية fossil اسمها
+
+613
+00:43:27,720 --> 00:43:31,480
+Electrophoresis في عندنا احنا اليوم حكينا عن تقنية
+
+614
+00:43:31,480 --> 00:43:34,760
+ال chromatography في عندنا تقنية تانية اسمها
+
+615
+00:43:34,760 --> 00:43:40,720
+Electrophoresis لنقارن بين التقانيتين بحكيلي بناء
+
+616
+00:43:40,720 --> 00:43:43,360
+على ال definition بناء على التعريف ال
+
+617
+00:43:43,360 --> 00:43:47,540
+chromatography معناها fossil خليط من عدة مكونات
+
+618
+00:43:47,540 --> 00:43:50,260
+بناء على two phases اللي ال stationary phase و ال
+
+619
+00:43:50,260 --> 00:43:54,650
+mobile phaseإذن تقنية الـ chromatography هي فصل
+
+620
+00:43:54,650 --> 00:43:58,010
+خليط من المواد بناء على ال stationary phase و ال
+
+621
+00:43:58,010 --> 00:44:04,050
+mobile phase أما ال electrophoresis هي فصل خليط من
+
+622
+00:44:04,050 --> 00:44:10,550
+عدة مكونات بناء على الخصائص الكهربائية على ال
+
+623
+00:44:10,550 --> 00:44:14,190
+electrical field هو فصل بناء على الخصائص
+
+624
+00:44:14,190 --> 00:44:16,890
+الكهربائية ال electrophoresis أما ال
+
+625
+00:44:16,890 --> 00:44:20,730
+chromatography هو فصل بناء على stationary phase و
+
+626
+00:44:20,730 --> 00:44:26,720
+mobile phaseالعينة اللى ممكن استخدمها في حالة ال
+
+627
+00:44:26,720 --> 00:44:31,020
+chromatography ايش العينات اللى بقدر افصلها ممكن
+
+628
+00:44:31,020 --> 00:44:35,120
+افصل عينات تكون liquid ممكن تكون solid ممكن gas
+
+629
+00:44:35,120 --> 00:44:41,100
+بيقدر يفصل عينات تكون سائلة ممكن صلبة ممكن غازية
+
+630
+00:44:41,100 --> 00:44:47,700
+ال electrophoresis بيقدر يفصل عينات تكون سائلة و
+
+631
+00:44:47,700 --> 00:44:52,710
+صلبة فقط بيقدرش يفصل الغازالـ Principle المبدأ
+
+632
+00:44:52,710 --> 00:44:55,990
+للكرومتографية حكى هنا إن المبدأ الكرومتографي الـ
+
+633
+00:44:55,990 --> 00:45:00,170
+Principle هو attraction with stationary phase و
+
+634
+00:45:00,170 --> 00:45:04,150
+solubility with mobile phase أما الـ
+
+635
+00:45:04,150 --> 00:45:07,910
+Electrophoresis هي فصل بناء على الـ electrical
+
+636
+00:45:07,910 --> 00:45:12,550
+field بناء على الخصائص الكهربائية بناء على ال
+
+637
+00:45:12,550 --> 00:45:17,530
+electrical properties إذن هو فصل بناء على الخصائص
+
+638
+00:45:17,530 --> 00:45:21,460
+الكهربائيةالـ Principle لأ للـ Electrophoresis
+
+639
+00:45:21,460 --> 00:45:24,840
+بناء على الخصائص الكهربائية بناء على الـ
+
+640
+00:45:24,840 --> 00:45:28,200
+Chromatography بناء على ال attraction with mobile
+
+641
+00:45:28,200 --> 00:45:32,180
+phase with stationary phase with solubility with
+
+642
+00:45:32,180 --> 00:45:33,600
+mobile phase
+
diff --git a/PL9fwy3NUQKwZ1Q7zOg1ObgufPMjRI3WY1/EDL86sMHiEI_raw.json b/PL9fwy3NUQKwZ1Q7zOg1ObgufPMjRI3WY1/EDL86sMHiEI_raw.json
new file mode 100644
index 0000000000000000000000000000000000000000..10da04d4fdfc0ff67fc900025d5112c317f92b72
--- /dev/null
+++ b/PL9fwy3NUQKwZ1Q7zOg1ObgufPMjRI3WY1/EDL86sMHiEI_raw.json
@@ -0,0 +1 @@
+{"segments": [{"id": 1, "seek": 2635, "start": 0.49, "end": 26.35, "text": "اليوم ان شاء الله بنحكي في معمل جديد اسمه ال chromatography ال chromatography هي تقنية لفصل الخليط من المواد إلى مكوناتها عندي خليط من المواد من عند المواد لأفصل كل مكون على حدة في البداية نحكي مقدمة عن هذه الطريقة و كيف تم اكتشافها", "tokens": [6027, 1829, 20498, 16472, 13412, 16606, 21984, 44945, 5016, 4117, 1829, 8978, 20449, 42213, 10874, 16254, 3215, 24525, 2304, 3224, 2423, 16209, 267, 5820, 2423, 16209, 267, 5820, 39896, 6055, 4587, 1863, 10632, 5296, 5172, 36520, 33962, 20292, 9566, 9154, 9673, 14407, 3215, 30731, 3714, 4117, 2407, 8315, 2655, 11296, 18871, 16254, 16490, 20292, 9566, 9154, 9673, 14407, 3215, 9154, 43242, 9673, 14407, 3215, 5296, 10721, 5172, 36520, 28242, 3714, 30544, 15844, 11331, 41891, 8978, 29739, 28259, 10632, 8717, 5016, 4117, 1829, 3714, 28543, 46007, 18871, 29538, 41950, 16572, 28671, 4032, 9122, 33911, 46811, 1975, 4117, 2655, 8592, 31845, 11296], "avg_logprob": 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بابا خصائصها بتكون برس material هي مادة مثقبة ال stationary phase هي مادة مثقبة برس material ليش؟ عشان تسمح بدخول السائل", "tokens": [9566, 3555, 3615, 995, 4032, 2288, 28671, 3714, 43020, 6055, 30544, 20328, 46152, 3660, 39896, 4032, 2288, 28671, 20328, 46152, 3660, 6055, 30544, 3714, 3215, 25957, 3660, 9154, 33962, 46538, 4724, 11622, 7435, 26108, 34051, 5551, 19528, 7649, 1829, 11536, 6225, 8592, 7649, 6055, 35571, 1829, 11296, 9154, 16712, 4117, 3794, 2288, 4032, 2304, 43020, 6055, 30544, 6225, 3555, 9640, 3660, 18871, 4724, 16758, 995, 16490, 9381, 16373, 9381, 11296, 39894, 30544, 4724, 2288, 3794, 2527, 39896, 3714, 18513, 3660, 3714, 12984, 4587, 49401, 2423, 30452, 5574, 39896, 3714, 18513, 3660, 3714, 12984, 4587, 49401, 4724, 2288, 3794, 2527, 32239, 8592, 22807, 6225, 8592, 7649, 6055, 38251, 5016, 47525, 9778, 12610, 21136, 16373, 1211], "avg_logprob": -0.14864129864651224, "compression_ratio": 1.8525345622119815, "no_speech_prob": 1.1920928955078125e-07, "words": 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هذه المكونات بيعطينا نقطة في مكان معين فالـ Mobile phase هو عبارة عن الـ solvent اسمه ممكن نحكي عنه الـ solvent أو الـluent هو بيكون liquid أو gas الـ mobile phase نبقى الستروزا stationary phase", "tokens": [9566, 12610, 19446, 31439, 4724, 32640, 8592, 1829, 21136, 16373, 1211, 4724, 33911, 36520, 20449, 40294, 33962, 20292, 9566, 32767, 4117, 2407, 8315, 47395, 15844, 39896, 19986, 3660, 8717, 4587, 41193, 28242, 4386, 28242, 3714, 4117, 2407, 11703, 1863, 9154, 29538, 9673, 4117, 2407, 8315, 2655, 4724, 40228, 9566, 1829, 8315, 8717, 47432, 3660, 8978, 3714, 41361, 20449, 9957, 6156, 6027, 39184, 22625, 5574, 31439, 6225, 3555, 9640, 3660, 18871, 2423, 39184, 33575, 24525, 2304, 3224, 3714, 43020, 8717, 5016, 4117, 1829, 18871, 3224, 2423, 39184, 33575, 34051, 2423, 39184, 43518, 31439, 4724, 1829, 30544, 6553, 34051, 4211, 2423, 39184, 6013, 5574, 8717, 3555, 4587, 7578, 2423, 14851, 32887, 11622, 995, 30452, 5574], "avg_logprob": -0.21271929837632597, "compression_ratio": 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"بغمر ال stationary phase في ال mobile phase بحكي لي بعدين ال mobile phase بيمر عبر ال stationary phase هيبدأ يمر داخل ال stationary phase الورقة أنا لما أغمرها في السائل بيمشي السائل فيها للأعلى فبيمر ال mobile phase عبر ال stationary phase", "tokens": [3555, 17082, 29973, 2423, 30452, 5574, 8978, 2423, 6013, 5574, 4724, 5016, 4117, 1829, 32239, 39182, 9957, 2423, 6013, 5574, 4724, 1829, 29973, 6225, 26890, 2423, 30452, 5574, 39896, 44510, 10721, 7251, 29973, 11778, 47283, 1211, 2423, 30452, 5574, 2423, 13063, 28671, 41850, 5296, 15042, 5551, 17082, 29973, 11296, 8978, 21136, 16373, 1211, 4724, 32640, 8592, 1829, 21136, 16373, 1211, 8978, 11296, 24976, 10721, 3615, 23942, 6156, 21292, 29973, 2423, 6013, 5574, 6225, 26890, 2423, 30452, 5574], "avg_logprob": -0.14663461633981803, "compression_ratio": 2.090909090909091, "no_speech_prob": 5.960464477539062e-07, "words": [{"start": 497.75000000000006, "end": 498.59000000000003, "word": "بغمر", "probability": 0.8421223958333334}, {"start": 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{"start": 546.13, "end": 546.65, "word": " لبعض", "probability": 0.98974609375}, {"start": 546.65, "end": 547.37, "word": " فهما", "probability": 0.77392578125}, {"start": 547.37, "end": 547.65, "word": " نفس", "probability": 0.98779296875}, {"start": 547.65, "end": 548.27, "word": " الخصائص", "probability": 0.983154296875}, {"start": 548.27, "end": 548.79, "word": " فبيدوبوا", "probability": 0.907421875}, {"start": 548.79, "end": 549.23, "word": " ببعض", "probability": 0.9434814453125}, {"start": 549.23, "end": 549.79, "word": " لذن", "probability": 0.480224609375}, {"start": 549.79, "end": 549.93, "word": " ال", "probability": 0.96484375}, {"start": 549.93, "end": 550.47, "word": " affinity", "probability": 0.8720703125}, {"start": 550.47, "end": 551.09, "word": " هي", "probability": 0.92041015625}, {"start": 551.09, "end": 551.77, "word": " التجاذب", "probability": 0.9659423828125}, {"start": 551.77, "end": 552.41, "word": " الطبيعي", "probability": 0.9844970703125}, {"start": 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{"start": 560.47, "end": 561.13, "word": " adhesion", "probability": 0.6778564453125}, {"start": 561.13, "end": 561.45, "word": " or", "probability": 0.409423828125}, {"start": 561.45, "end": 562.09, "word": " stickness", "probability": 0.60986328125}, {"start": 562.09, "end": 562.29, "word": " of", "probability": 0.96337890625}, {"start": 562.29, "end": 562.93, "word": " substance", "probability": 0.76025390625}, {"start": 562.93, "end": 563.17, "word": " to", "probability": 0.955078125}, {"start": 563.17, "end": 563.37, "word": " the", "probability": 0.890625}, {"start": 563.37, "end": 563.79, "word": " surface", "probability": 0.86474609375}, {"start": 563.79, "end": 564.01, "word": " of", "probability": 0.982421875}, {"start": 564.01, "end": 564.35, "word": " another", "probability": 0.93798828125}, {"start": 564.35, "end": 564.91, "word": " substance", "probability": 0.96728515625}, {"start": 564.91, "end": 566.53, "word": " والتصاق", "probability": 0.8396809895833334}, {"start": 566.53, "end": 567.25, "word": " مادة", "probability": 0.9588216145833334}, {"start": 567.25, "end": 567.57, "word": " على", "probability": 0.96630859375}, {"start": 567.57, "end": 568.05, "word": " سطح", "probability": 0.9871419270833334}, {"start": 568.05, "end": 568.83, "word": " مادة", "probability": 0.97900390625}, {"start": 568.83, "end": 569.61, "word": " أخرى", "probability": 0.97705078125}], "temperature": 1.0}, {"id": 27, "seek": 57937, "start": 569.95, "end": 579.37, "text": "التصاق مادة على سطح مادة أخرى التصاق المادة بس على السطح بدون اختراق التركيب الداخلي للمادة هاي ال absorption", "tokens": [6027, 2655, 9381, 995, 4587, 3714, 18513, 3660, 15844, 8608, 9566, 5016, 3714, 18513, 3660, 5551, 34740, 7578, 16712, 9381, 995, 4587, 9673, 18513, 3660, 4724, 3794, 15844, 21136, 9566, 5016, 47525, 11536, 1975, 46456, 23557, 4587, 16712, 31747, 1829, 3555, 32748, 47283, 20292, 5296, 19528, 18513, 3660, 8032, 47302, 2423, 27557], "avg_logprob": -0.09758254660750335, "compression_ratio": 1.5702479338842976, "no_speech_prob": 5.960464477539063e-08, "words": [{"start": 569.95, "end": 570.87, "word": "التصاق", "probability": 0.96796875}, {"start": 570.87, "end": 571.53, "word": " مادة", "probability": 0.9534505208333334}, {"start": 571.53, "end": 571.73, "word": " على", "probability": 0.892578125}, {"start": 571.73, "end": 572.27, "word": " سطح", "probability": 0.9847005208333334}, {"start": 572.27, "end": 572.83, "word": " مادة", "probability": 0.9778645833333334}, {"start": 572.83, "end": 573.39, "word": " أخرى", "probability": 0.9609375}, {"start": 573.39, "end": 574.05, "word": " التصاق", "probability": 0.9215087890625}, {"start": 574.05, "end": 574.51, "word": " المادة", "probability": 0.9659830729166666}, {"start": 574.51, "end": 574.71, "word": " بس", "probability": 0.971923828125}, {"start": 574.71, "end": 574.89, "word": " على", "probability": 0.90966796875}, {"start": 574.89, "end": 575.39, "word": " السطح", "probability": 0.9851888020833334}, {"start": 575.39, "end": 575.91, "word": " بدون", "probability": 0.975341796875}, {"start": 575.91, "end": 576.43, "word": " اختراق", "probability": 0.9276123046875}, {"start": 576.43, "end": 576.93, "word": " التركيب", "probability": 0.9893798828125}, {"start": 576.93, "end": 577.43, "word": " الداخلي", "probability": 0.9453125}, {"start": 577.43, "end": 578.09, "word": " للمادة", "probability": 0.85968017578125}, {"start": 578.09, "end": 578.79, "word": " هاي", "probability": 0.6224365234375}, {"start": 578.79, "end": 578.89, "word": " ال", "probability": 0.9912109375}, {"start": 578.89, "end": 579.37, "word": " absorption", "probability": 0.303955078125}], "temperature": 1.0}, {"id": 28, "seek": 60666, "start": 579.82, "end": 606.66, "text": "absorption التصاق مادة على سطح مادة أخرى absorption معناها أن المادة تخترق التركيب الداخلي للمادة الأخرى مش التصاق فقط على السطح وإنما اختراق التركيب الداخلي للمادة فيها نوعين رابط يوتيوب هاي بوضح الفرق بين ال absorption و ال absorption إذا حبين تطلعوا عليه", "tokens": [17243, 284, 1695, 16712, 9381, 995, 4587, 3714, 18513, 3660, 15844, 8608, 9566, 5016, 3714, 18513, 3660, 5551, 34740, 7578, 27557, 20449, 8315, 11296, 14739, 9673, 18513, 3660, 6055, 46456, 2288, 4587, 16712, 31747, 1829, 3555, 32748, 47283, 20292, 5296, 19528, 18513, 3660, 16247, 34740, 7578, 37893, 16712, 9381, 995, 4587, 6156, 47432, 15844, 21136, 9566, 5016, 4032, 28814, 1863, 15042, 1975, 46456, 23557, 4587, 16712, 31747, 1829, 3555, 32748, 47283, 20292, 5296, 19528, 18513, 3660, 8978, 11296, 8717, 45367, 9957, 12602, 16758, 9566, 7251, 2407, 31371, 37746, 8032, 47302, 4724, 2407, 11242, 5016, 27188, 2288, 4587, 49374, 2423, 27557, 4032, 2423, 27557, 11933, 15730, 11331, 3555, 9957, 6055, 9566, 1211, 3615, 14407, 47356], "avg_logprob": -0.16032608099605727, "compression_ratio": 2.12807881773399, "no_speech_prob": 4.827976226806641e-06, "words": [{"start": 579.82, "end": 580.64, "word": "absorption", "probability": 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0.9807942708333334}, {"start": 596.88, "end": 598.56, "word": " للمادة", "probability": 0.916015625}, {"start": 598.56, "end": 599.42, "word": " فيها", "probability": 0.76123046875}, {"start": 599.42, "end": 599.68, "word": " نوعين", "probability": 0.2781982421875}, {"start": 599.68, "end": 600.1, "word": " رابط", "probability": 0.876953125}, {"start": 600.1, "end": 600.66, "word": " يوتيوب", "probability": 0.8707275390625}, {"start": 600.66, "end": 600.92, "word": " هاي", "probability": 0.4056396484375}, {"start": 600.92, "end": 601.42, "word": " بوضح", "probability": 0.8414306640625}, {"start": 601.42, "end": 601.82, "word": " الفرق", "probability": 0.9890950520833334}, {"start": 601.82, "end": 602.02, "word": " بين", "probability": 0.818359375}, {"start": 602.02, "end": 602.16, "word": " ال", "probability": 0.74853515625}, {"start": 602.16, "end": 602.86, "word": " absorption", "probability": 0.8544921875}, {"start": 602.86, "end": 603.32, "word": " و", "probability": 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0.89990234375}], "temperature": 1.0}, {"id": 51, "seek": 106598, "start": 1045.98, "end": 1065.98, "text": "هي هتلزج في ال stationary phase مش هتطلع لفوق و ال non-polar هي اللي هتطلع أسرع للأعلى لكن لو ال stationary phase is non-polar إذا ال non-polar هي اللي هتتجادب مع ال stationary و تضلها تحت و ال polar هو اللي هيصعد للأعلى بقوة أعلى", "tokens": [3224, 1829, 8032, 2655, 1211, 11622, 7435, 8978, 2423, 30452, 5574, 37893, 8032, 2655, 9566, 1211, 3615, 5296, 5172, 30543, 4032, 2423, 2107, 12, 12892, 289, 39896, 13672, 1829, 8032, 2655, 9566, 1211, 3615, 5551, 3794, 2288, 3615, 24976, 10721, 3615, 23942, 44381, 45164, 2423, 30452, 5574, 307, 2107, 12, 12892, 289, 11933, 15730, 2423, 2107, 12, 12892, 289, 39896, 13672, 1829, 8032, 2655, 2655, 7435, 18513, 3555, 20449, 2423, 30452, 4032, 6055, 11242, 1211, 11296, 6055, 33753, 4032, 2423, 12367, 31439, 13672, 1829, 39896, 9381, 22488, 24976, 10721, 3615, 23942, 4724, 4587, 2407, 3660, 5551, 3615, 23942], "avg_logprob": 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0.88134765625}, {"start": 1056.26, "end": 1056.5, "word": " is", "probability": 0.8740234375}, {"start": 1056.5, "end": 1056.84, "word": " non", "probability": 0.98046875}, {"start": 1056.84, "end": 1057.42, "word": "-polar", "probability": 0.8779296875}, {"start": 1057.42, "end": 1057.88, "word": " إذا", "probability": 0.634033203125}, {"start": 1057.88, "end": 1058.1, "word": " ال", "probability": 0.52587890625}, {"start": 1058.1, "end": 1058.34, "word": " non", "probability": 0.93359375}, {"start": 1058.34, "end": 1058.8, "word": "-polar", "probability": 0.97509765625}, {"start": 1058.8, "end": 1059.02, "word": " هي", "probability": 0.943359375}, {"start": 1059.02, "end": 1059.2, "word": " اللي", "probability": 0.989013671875}, {"start": 1059.2, "end": 1060.02, "word": " هتتجادب", "probability": 0.8951416015625}, {"start": 1060.02, "end": 1060.18, "word": " مع", "probability": 0.97802734375}, {"start": 1060.18, "end": 1060.32, "word": " ال", "probability": 0.978515625}, {"start": 1060.32, "end": 1060.68, "word": " stationary", "probability": 0.9833984375}, {"start": 1060.68, "end": 1060.98, "word": " و", "probability": 0.46826171875}, {"start": 1060.98, "end": 1061.26, "word": " تضلها", "probability": 0.6781005859375}, {"start": 1061.26, "end": 1061.74, "word": " تحت", "probability": 0.99560546875}, {"start": 1061.74, "end": 1062.26, "word": " و", "probability": 0.701171875}, {"start": 1062.26, "end": 1062.52, "word": " ال", "probability": 0.93603515625}, {"start": 1062.52, "end": 1062.9, "word": " polar", "probability": 0.86474609375}, {"start": 1062.9, "end": 1063.16, "word": " هو", "probability": 0.98876953125}, {"start": 1063.16, "end": 1063.32, "word": " اللي", "probability": 0.98046875}, {"start": 1063.32, "end": 1063.84, "word": " هيصعد", "probability": 0.884765625}, {"start": 1063.84, "end": 1064.4, "word": " للأعلى", "probability": 0.9404296875}, {"start": 1064.4, "end": 1065.58, "word": " بقوة", "probability": 0.9859619140625}, {"start": 1065.58, 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1087.56, "end": 1088.34, "word": " face", "probability": 0.53759765625}], "temperature": 1.0}, {"id": 53, "seek": 110528, "start": 1088.86, "end": 1105.28, "text": "أول عامل الـ solubility with mobile face اللي مكتوب هنا الـ solvent الـ solvent معناه ال mobile face إذا كان بيدوب في ال mobile face له نفس خصائص ال mobile face إذا هو هيصعد للأعلى العامل التاني ال attraction", "tokens": [10721, 12610, 6225, 10943, 1211, 2423, 39184, 1404, 836, 1140, 365, 6013, 1851, 13672, 1829, 3714, 4117, 2655, 37746, 34105, 2423, 39184, 33575, 2423, 39184, 33575, 20449, 8315, 3224, 2423, 6013, 1851, 11933, 15730, 25961, 4724, 25708, 37746, 8978, 2423, 6013, 1851, 46740, 8717, 36178, 16490, 9381, 16373, 9381, 2423, 6013, 1851, 11933, 15730, 31439, 39896, 9381, 22488, 24976, 10721, 3615, 23942, 18863, 10943, 1211, 16712, 7649, 1829, 2423, 17672], "avg_logprob": -0.23811619676334758, "compression_ratio": 1.7241379310344827, "no_speech_prob": 2.384185791015625e-07, "words": [{"start": 1088.86, "end": 1089.3, "word": "أول", "probability": 0.91796875}, {"start": 1089.3, "end": 1089.8, "word": " عامل", "probability": 0.9899088541666666}, {"start": 1089.8, "end": 1090.06, "word": " الـ", "probability": 0.314697265625}, {"start": 1090.06, "end": 1090.72, "word": " solubility", "probability": 0.7569986979166666}, {"start": 1090.72, "end": 1090.96, "word": " with", "probability": 0.7734375}, {"start": 1090.96, "end": 1091.42, "word": " mobile", "probability": 0.95263671875}, {"start": 1091.42, "end": 1092.26, "word": " face", "probability": 0.466064453125}, {"start": 1092.26, "end": 1092.62, "word": " اللي", "probability": 0.74951171875}, {"start": 1092.62, "end": 1093.06, "word": " مكتوب", "probability": 0.92138671875}, {"start": 1093.06, "end": 1093.24, "word": " هنا", "probability": 0.779296875}, {"start": 1093.24, "end": 1093.4, "word": " الـ", "probability": 0.4093017578125}, {"start": 1093.4, "end": 1093.76, "word": " solvent", "probability": 0.5712890625}, {"start": 1093.76, "end": 1094.04, "word": " الـ", "probability": 0.4957275390625}, {"start": 1094.04, "end": 1094.28, "word": " solvent", "probability": 0.81201171875}, {"start": 1094.28, "end": 1094.68, "word": " معناه", "probability": 0.8580729166666666}, {"start": 1094.68, "end": 1094.76, "word": " ال", "probability": 0.88525390625}, {"start": 1094.76, "end": 1095.08, "word": " mobile", "probability": 0.414794921875}, {"start": 1095.08, "end": 1095.44, "word": " face", "probability": 0.8798828125}, {"start": 1095.44, "end": 1096.12, "word": " إذا", "probability": 0.88232421875}, {"start": 1096.12, "end": 1096.46, "word": " كان", "probability": 0.99462890625}, {"start": 1096.46, "end": 1097.08, "word": " بيدوب", "probability": 0.7722981770833334}, {"start": 1097.08, "end": 1097.26, "word": " في", "probability": 0.92919921875}, {"start": 1097.26, "end": 1097.36, "word": " ال", "probability": 0.96533203125}, {"start": 1097.36, "end": 1097.68, "word": " mobile", "probability": 0.8828125}, {"start": 1097.68, 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mobile phase ال chromatography مقسم إلى جزئين بناء على ال stationary إليه تقسيمة و بناء على ال mobile إليه تقسيمة بناء على ال mobile phase فيه نوعين إما بستخدم ال mobile phase يكون gas، غاز أو بكون liquid", "tokens": [20467, 295, 16209, 267, 5820, 5551, 4587, 3794, 10943, 5296, 39184, 16209, 267, 5820, 5296, 7435, 3224, 3660, 2423, 16209, 267, 5820, 3714, 4587, 38251, 30731, 12174, 38251, 9957, 44945, 16606, 15844, 2423, 30452, 4032, 44945, 16606, 15844, 2423, 6013, 5574, 2423, 16209, 267, 5820, 3714, 4587, 38251, 30731, 10874, 11622, 19986, 9957, 44945, 16606, 15844, 2423, 30452, 11933, 20292, 3224, 6055, 4587, 3794, 32640, 3660, 4032, 44945, 16606, 15844, 2423, 6013, 11933, 20292, 3224, 6055, 4587, 3794, 32640, 3660, 44945, 16606, 15844, 2423, 6013, 5574, 8978, 3224, 8717, 45367, 9957, 11933, 15042, 4724, 14851, 9778, 40448, 2423, 6013, 5574, 7251, 30544, 4211, 12399, 32771, 31377, 34051, 4724, 30544, 6553], "avg_logprob": -0.2010135137820029, "compression_ratio": 2.336683417085427, 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على ال stationary face في منه column على شكل عمود و في منه planner على شكل سطح مستوي اذا بناء على ال stationary face في منه اتنين column عمود او planner مستوي و ال planner في منه thin layer chromatography و في منه paper chromatography", "tokens": [3555, 1863, 16606, 15844, 2423, 30452, 1851, 8978, 9154, 3224, 7738, 15844, 13412, 28820, 6225, 2304, 23328, 4032, 8978, 9154, 3224, 31268, 15844, 13412, 28820, 8608, 9566, 5016, 3714, 14851, 45865, 1975, 15730, 44945, 16606, 15844, 2423, 30452, 1851, 8978, 9154, 3224, 1975, 2655, 1863, 9957, 7738, 6225, 2304, 23328, 1975, 2407, 31268, 3714, 14851, 45865, 4032, 2423, 31268, 8978, 9154, 3224, 5862, 4583, 16209, 267, 5820, 4032, 8978, 9154, 3224, 3035, 16209, 267, 5820], "avg_logprob": -0.21607730243551104, "compression_ratio": 2.0, "no_speech_prob": 0.0, "words": [{"start": 1570.47, "end": 1570.85, "word": "بناء", "probability": 0.7980143229166666}, {"start": 1570.85, "end": 1571.03, "word": " على", "probability": 0.393310546875}, {"start": 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chromatography بناء على ال mobile phase في منها liquid و في منها gas", "tokens": [3224, 8315, 10874, 3215, 12610, 4724, 1829, 2407, 11242, 5016, 16472, 3224, 1975, 2407, 16472, 3224, 3714, 1211, 9778, 9381, 8032, 47302, 2423, 4137, 9673, 9566, 1211, 37746, 9154, 11296, 1975, 9381, 15040, 9154, 11296, 16472, 3224, 2423, 16209, 267, 5820, 6055, 4587, 3794, 32640, 2423, 16209, 267, 5820, 44945, 16606, 15844, 2423, 6013, 5574, 16472, 8978, 43242, 8315, 6553, 16209, 267, 5820, 4032, 4211, 16209, 267, 5820, 4724, 3794, 5296, 15730, 6055, 4587, 3794, 32640, 2423, 16209, 267, 5820, 44945, 16606, 15844, 2423, 6013, 5574, 8978, 9154, 11296, 6553, 4032, 8978, 9154, 11296, 4211], "avg_logprob": -0.17187499906867743, "compression_ratio": 2.0526315789473686, "no_speech_prob": 0.0, "words": [{"start": 1593.05, "end": 1593.39, "word": "هنا", "probability": 0.93896484375}, {"start": 1593.39, "end": 1593.89, "word": " جدول", "probability": 0.9529622395833334}, {"start": 1593.89, "end": 1594.41, "word": 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" أنتوا", "probability": 0.7176920572916666}, {"start": 2417.12, "end": 2417.46, "word": " شايفين", "probability": 0.9793701171875}, {"start": 2417.46, "end": 2417.68, "word": " هنا", "probability": 0.728515625}, {"start": 2417.68, "end": 2418.12, "word": " مثلا", "probability": 0.98486328125}, {"start": 2418.12, "end": 2419.16, "word": " أجد", "probability": 0.7137044270833334}, {"start": 2419.16, "end": 2419.44, "word": " خط", "probability": 0.989990234375}, {"start": 2419.44, "end": 2419.9, "word": " البداية", "probability": 0.9651692708333334}, {"start": 2419.9, "end": 2420.06, "word": " اللي", "probability": 0.93896484375}, {"start": 2420.06, "end": 2420.2, "word": " هو", "probability": 0.84716796875}, {"start": 2420.2, "end": 2420.56, "word": " تحت", "probability": 0.98779296875}, {"start": 2420.56, "end": 2420.78, "word": " أنا", "probability": 0.71142578125}, {"start": 2420.78, "end": 2421.08, "word": " حاطيت", "probability": 0.774658203125}, {"start": 2421.08, "end": 2421.48, "word": " الخليط", "probability": 0.95751953125}, {"start": 2421.48, "end": 2421.64, "word": " من", "probability": 0.99072265625}, {"start": 2421.64, "end": 2421.98, "word": " الخط", "probability": 0.98828125}, {"start": 2421.98, "end": 2422.16, "word": " اللي", "probability": 0.98583984375}, {"start": 2422.16, "end": 2422.56, "word": " تحت", "probability": 0.97998046875}, {"start": 2422.56, "end": 2423.62, "word": " النقطة", "probability": 0.8972981770833334}, {"start": 2423.62, "end": 2423.98, "word": " الحمراء", "probability": 0.6875}, {"start": 2423.98, "end": 2424.38, "word": " مثلا", "probability": 0.98486328125}, {"start": 2424.38, "end": 2425.1, "word": " بجيس", "probability": 0.9111328125}, {"start": 2425.1, "end": 2425.58, "word": " المسافة", "probability": 0.9810791015625}, {"start": 2425.58, "end": 2426.2, "word": " بالمسطرة", "probability": 0.852490234375}, {"start": 2426.2, "end": 2426.4, "word": " من", "probability": 0.9912109375}, {"start": 2426.4, "end": 2426.86, 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أكسوجين", "probability": 0.86337890625}, {"start": 2524.69, "end": 2525.27, "word": " والسطح", "probability": 0.886962890625}, {"start": 2525.27, "end": 2525.65, "word": " برضه", "probability": 0.9822591145833334}, {"start": 2525.65, "end": 2526.11, "word": " قوات", "probability": 0.7049153645833334}, {"start": 2526.11, "end": 2526.89, "word": " فهيكون", "probability": 0.918212890625}, {"start": 2526.89, "end": 2527.27, "word": " بولر", "probability": 0.9656575520833334}, {"start": 2527.27, "end": 2527.65, "word": " زيها", "probability": 0.9889322916666666}], "temperature": 1.0}, {"id": 114, "seek": 255506, "start": 2528.68, "end": 2555.06, "text": "بشكل عام إن الـ stationary phase في الـ thin layer كروماتجرافي عبارة عن سيليكون عبارة عن سيليكا جيل و السيليكا على سطحها OH فالـ OH يعني polar فالـ thin layer كروماتجرافي لو حكيتلك استخدام التقنية الكروماتجرافي الـ thin layer كروماتجرافي بدك تفهمي لحالك إن الـ stationary phase polar و ال mobile phase non polar", "tokens": [3555, 8592, 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{"start": 2529.1, "end": 2529.52, "word": " عام", "probability": 0.998291015625}, {"start": 2529.52, "end": 2529.68, "word": " إن", "probability": 0.316162109375}, {"start": 2529.68, "end": 2529.96, "word": " الـ", "probability": 0.6903076171875}, {"start": 2529.96, "end": 2530.3, "word": " stationary", "probability": 0.438232421875}, {"start": 2530.3, "end": 2530.84, "word": " phase", "probability": 0.84814453125}, {"start": 2530.84, "end": 2531.06, "word": " في", "probability": 0.88671875}, {"start": 2531.06, "end": 2531.24, "word": " الـ", "probability": 0.83642578125}, {"start": 2531.24, "end": 2531.4, "word": " thin", "probability": 0.599609375}, {"start": 2531.4, "end": 2531.72, "word": " layer", "probability": 0.89208984375}, {"start": 2531.72, "end": 2532.68, "word": " كروماتجرافي", "probability": 0.6799665178571429}, {"start": 2532.68, "end": 2533.62, "word": " عبارة", "probability": 0.84979248046875}, {"start": 2533.62, "end": 2533.88, "word": " عن", "probability": 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"probability": 0.849853515625}, {"start": 2547.5, "end": 2547.72, "word": " thin", "probability": 0.8544921875}, {"start": 2547.72, "end": 2548.18, "word": " layer", "probability": 0.94921875}, {"start": 2548.18, "end": 2549.04, "word": " كروماتجرافي", "probability": 0.9740513392857143}, {"start": 2549.04, "end": 2549.64, "word": " بدك", "probability": 0.6929931640625}, {"start": 2549.64, "end": 2550.18, "word": " تفهمي", "probability": 0.96923828125}, {"start": 2550.18, "end": 2550.94, "word": " لحالك", "probability": 0.9879150390625}, {"start": 2550.94, "end": 2551.34, "word": " إن", "probability": 0.818359375}, {"start": 2551.34, "end": 2551.64, "word": " الـ", "probability": 0.7183837890625}, {"start": 2551.64, "end": 2551.96, "word": " stationary", "probability": 0.9599609375}, {"start": 2551.96, "end": 2552.54, "word": " phase", "probability": 0.96533203125}, {"start": 2552.54, "end": 2553.0, "word": " polar", "probability": 0.57373046875}, {"start": 2553.0, "end": 2553.62, 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"word": " solid", "probability": 0.9453125}, {"start": 2674.1, "end": 2674.6, "word": " ممكن", "probability": 0.935546875}, {"start": 2674.6, "end": 2675.12, "word": " gas", "probability": 0.69140625}, {"start": 2675.12, "end": 2675.82, "word": " بيقدر", "probability": 0.92822265625}, {"start": 2675.82, "end": 2676.22, "word": " يفصل", "probability": 0.9737955729166666}, {"start": 2676.22, "end": 2676.66, "word": " عينات", "probability": 0.9261474609375}, {"start": 2676.66, "end": 2676.98, "word": " تكون", "probability": 0.98486328125}, {"start": 2676.98, "end": 2677.58, "word": " سائلة", "probability": 0.9453125}, {"start": 2677.58, "end": 2678.34, "word": " ممكن", "probability": 0.964111328125}, {"start": 2678.34, "end": 2678.9, "word": " صلبة", "probability": 0.93798828125}, {"start": 2678.9, "end": 2679.5, "word": " ممكن", "probability": 0.936767578125}, {"start": 2679.5, "end": 2681.1, "word": " غازية", "probability": 0.8966471354166666}, {"start": 2681.1, "end": 2681.74, "word": " ال", "probability": 0.958984375}, {"start": 2681.74, "end": 2683.1, "word": " electrophoresis", "probability": 0.780029296875}, {"start": 2683.1, "end": 2683.96, "word": " بيقدر", "probability": 0.980224609375}, {"start": 2683.96, "end": 2684.58, "word": " يفصل", "probability": 0.98095703125}, {"start": 2684.58, "end": 2685.88, "word": " عينات", "probability": 0.9566650390625}, {"start": 2685.88, "end": 2686.48, "word": " تكون", "probability": 0.98974609375}, {"start": 2686.48, "end": 2687.32, "word": " سائلة", "probability": 0.9832356770833334}, {"start": 2687.32, "end": 2687.7, "word": " و", "probability": 0.94970703125}, {"start": 2687.7, "end": 2688.18, "word": " صلبة", "probability": 0.8665364583333334}, {"start": 2688.18, "end": 2688.58, "word": " فقط", "probability": 0.99658203125}, {"start": 2688.58, "end": 2689.22, "word": " بيقدرش", "probability": 0.90595703125}, {"start": 2689.22, "end": 2689.7, "word": " يفصل", "probability": 0.9814453125}, {"start": 2689.7, "end": 2690.38, "word": " الغاز", "probability": 0.9837239583333334}], "temperature": 1.0}, {"id": 120, "seek": 271847, "start": 2691.29, "end": 2718.47, "text": "الـ Principle المبدأ للكرومتографية حكى هنا إن المبدأ الكرومتографي الـ Principle هو attraction with stationary phase و solubility with mobile phase أما الـ Electrophoresis هي فصل بناء على الـ electrical field بناء على الخصائص الكهربائية بناء على ال electrical properties إذن هو فصل بناء على الخصائص الكهربائية", "tokens": [6027, 39184, 38372, 781, 9673, 44510, 10721, 5296, 23275, 2288, 20498, 2655, 29046, 10632, 11331, 4117, 7578, 34105, 36145, 9673, 44510, 10721, 33251, 2288, 20498, 2655, 29046, 1829, 2423, 39184, 38372, 781, 31439, 17672, 365, 30452, 5574, 4032, 1404, 836, 1140, 365, 6013, 5574, 5551, 15042, 2423, 39184, 12575, 11741, 2706, 271, 39896, 6156, 36520, 44945, 16606, 15844, 2423, 39184, 12147, 2519, 44945, 16606, 15844, 33962, 9381, 16373, 9381, 33251, 3224, 25513, 16373, 10632, 44945, 16606, 15844, 2423, 12147, 7221, 11933, 8848, 1863, 31439, 6156, 36520, 44945, 16606, 15844, 33962, 9381, 16373, 9381, 33251, 3224, 25513, 16373, 10632], "avg_logprob": -0.2040719645793992, "compression_ratio": 1.8677685950413223, "no_speech_prob": 0.0, "words": [{"start": 2691.29, "end": 2691.61, "word": "الـ", "probability": 0.510986328125}, {"start": 2691.61, "end": 2692.09, "word": " Principle", "probability": 0.69873046875}, {"start": 2692.09, "end": 2692.71, "word": " المبدأ", "probability": 0.958984375}, {"start": 2692.71, "end": 2693.67, "word": " للكرومتографية", "probability": 0.5889369419642857}, {"start": 2693.67, "end": 2693.99, "word": " حكى", "probability": 0.7447916666666666}, {"start": 2693.99, "end": 2694.21, "word": " هنا", "probability": 0.97265625}, {"start": 2694.21, "end": 2694.47, "word": " إن", "probability": 0.52392578125}, {"start": 2694.47, "end": 2695.01, "word": " المبدأ", "probability": 0.9054361979166666}, {"start": 2695.01, "end": 2695.81, "word": " الكرومتографي", "probability": 0.9146321614583334}, {"start": 2695.81, "end": 2695.99, "word": " الـ", "probability": 0.64306640625}, {"start": 2695.99, "end": 2696.47, "word": " Principle", "probability": 0.771728515625}, {"start": 2696.47, "end": 2696.79, "word": " هو", "probability": 0.9736328125}, {"start": 2696.79, "end": 2697.83, "word": " attraction", "probability": 0.52392578125}, {"start": 2697.83, "end": 2698.39, "word": " with", "probability": 0.9326171875}, {"start": 2698.39, "end": 2699.25, "word": " stationary", "probability": 0.92919921875}, {"start": 2699.25, "end": 2699.71, "word": " phase", "probability": 0.8056640625}, {"start": 2699.71, "end": 2700.17, "word": " و", "probability": 0.85791015625}, {"start": 2700.17, "end": 2701.17, "word": " solubility", "probability": 0.8299153645833334}, {"start": 2701.17, "end": 2701.83, "word": " with", "probability": 0.94091796875}, {"start": 2701.83, "end": 2702.45, "word": " mobile", "probability": 0.97314453125}, {"start": 2702.45, "end": 2702.87, "word": " phase", "probability": 0.94580078125}, {"start": 2702.87, "end": 2703.79, "word": " أما", "probability": 0.964599609375}, {"start": 2703.79, "end": 2704.15, "word": " الـ", "probability": 0.6043701171875}, {"start": 2704.15, "end": 2705.09, "word": " Electrophoresis", "probability": 0.8966064453125}, {"start": 2705.09, "end": 2705.59, "word": " هي", "probability": 0.88232421875}, {"start": 2705.59, "end": 2706.03, "word": " فصل", "probability": 0.9453125}, {"start": 2706.03, "end": 2706.47, "word": " بناء", "probability": 0.97607421875}, {"start": 2706.47, "end": 2706.95, "word": " على", "probability": 0.73876953125}, {"start": 2706.95, "end": 2707.27, "word": " الـ", "probability": 0.80810546875}, {"start": 2707.27, "end": 2707.91, "word": " electrical", "probability": 0.77099609375}, {"start": 2707.91, "end": 2708.55, "word": " field", "probability": 0.95166015625}, {"start": 2708.55, "end": 2709.17, "word": " بناء", "probability": 0.930419921875}, {"start": 2709.17, "end": 2709.51, "word": " على", "probability": 0.94970703125}, {"start": 2709.51, "end": 2710.23, "word": " الخصائص", "probability": 0.988037109375}, {"start": 2710.23, "end": 2711.13, "word": " الكهربائية", "probability": 0.9734375}, {"start": 2711.13, "end": 2711.89, "word": " بناء", "probability": 0.9345703125}, {"start": 2711.89, "end": 2712.27, "word": " على", "probability": 0.947265625}, {"start": 2712.27, "end": 2712.55, "word": " ال", "probability": 0.9775390625}, {"start": 2712.55, "end": 2713.19, "word": " electrical", "probability": 0.52880859375}, {"start": 2713.19, "end": 2714.07, "word": " properties", "probability": 0.18115234375}, {"start": 2714.07, "end": 2715.01, "word": " إذن", "probability": 0.6822916666666666}, {"start": 2715.01, "end": 2715.17, "word": " هو", "probability": 0.8544921875}, {"start": 2715.17, "end": 2715.63, "word": " فصل", "probability": 0.98974609375}, {"start": 2715.63, "end": 2716.19, "word": " بناء", "probability": 0.986328125}, {"start": 2716.19, "end": 2716.59, "word": " على", "probability": 0.951171875}, {"start": 2716.59, "end": 2717.53, "word": " الخصائص", "probability": 0.99609375}, {"start": 2717.53, "end": 2718.47, "word": " الكهربائية", "probability": 0.99306640625}], "temperature": 1.0}, {"id": 121, "seek": 273360, "start": 2719.0, "end": 2733.6, "text": "الـ Principle لأ للـ Electrophoresis بناء على الخصائص الكهربائية بناء على الـ Chromatography بناء على ال attraction with mobile phase with stationary phase with solubility with mobile phase", "tokens": [6027, 39184, 38372, 781, 5296, 10721, 24976, 39184, 12575, 11741, 2706, 271, 44945, 16606, 15844, 33962, 9381, 16373, 9381, 33251, 3224, 25513, 16373, 10632, 44945, 16606, 15844, 2423, 39184, 1721, 34295, 5820, 44945, 16606, 15844, 2423, 17672, 365, 6013, 5574, 365, 30452, 5574, 365, 1404, 836, 1140, 365, 6013, 5574], "avg_logprob": -0.2506127544477874, "compression_ratio": 1.5286624203821657, "no_speech_prob": 0.0, "words": [{"start": 2719.0, "end": 2719.32, "word": "الـ", "probability": 0.718505859375}, {"start": 2719.32, "end": 2720.06, "word": " Principle", "probability": 0.5772705078125}, {"start": 2720.06, "end": 2720.36, "word": " لأ", "probability": 0.733642578125}, {"start": 2720.36, "end": 2720.54, "word": " للـ", "probability": 0.722412109375}, {"start": 2720.54, "end": 2721.46, "word": " Electrophoresis", "probability": 0.927734375}, {"start": 2721.46, "end": 2721.7, "word": " بناء", "probability": 0.826171875}, {"start": 2721.7, "end": 2721.8, "word": " على", "probability": 0.6748046875}, {"start": 2721.8, "end": 2722.3, "word": " الخصائص", "probability": 0.94921875}, {"start": 2722.3, "end": 2723.12, "word": " الكهربائية", "probability": 0.92666015625}, {"start": 2723.12, "end": 2723.78, "word": " بناء", "probability": 0.645263671875}, {"start": 2723.78, "end": 2723.94, "word": " على", "probability": 0.5966796875}, {"start": 2723.94, "end": 2724.84, "word": " الـ", "probability": 0.5970458984375}, {"start": 2724.84, "end": 2725.78, "word": " Chromatography", "probability": 0.8292643229166666}, {"start": 2725.78, "end": 2726.22, "word": " بناء", "probability": 0.954345703125}, {"start": 2726.22, "end": 2726.48, "word": " على", "probability": 0.966796875}, {"start": 2726.48, "end": 2726.62, "word": " ال", "probability": 0.9228515625}, {"start": 2726.62, "end": 2727.36, "word": " attraction", "probability": 0.69677734375}, {"start": 2727.36, "end": 2727.66, "word": " with", "probability": 0.8974609375}, {"start": 2727.66, "end": 2728.2, "word": " mobile", "probability": 0.923828125}, {"start": 2728.2, "end": 2728.66, "word": " phase", "probability": 0.51220703125}, {"start": 2728.66, "end": 2729.14, "word": " with", "probability": 0.54150390625}, {"start": 2729.14, "end": 2729.7, "word": " stationary", "probability": 0.9638671875}, {"start": 2729.7, "end": 2730.28, "word": " phase", "probability": 0.96875}, {"start": 2730.28, "end": 2730.82, "word": " with", "probability": 0.173583984375}, {"start": 2730.82, "end": 2731.72, "word": " solubility", "probability": 0.9637044270833334}, {"start": 2731.72, "end": 2732.18, "word": " with", "probability": 0.91259765625}, {"start": 2732.18, "end": 2732.96, "word": " mobile", "probability": 0.953125}, {"start": 2732.96, "end": 2733.6, "word": " phase", "probability": 0.97021484375}], "temperature": 1.0}], "language": "ar", "language_probability": 1.0, "duration": 2734.7998125, "duration_after_vad": 2659.720624999988}
\ No newline at end of file
diff --git a/PL9fwy3NUQKwZ1Q7zOg1ObgufPMjRI3WY1/EDL86sMHiEI_raw.srt b/PL9fwy3NUQKwZ1Q7zOg1ObgufPMjRI3WY1/EDL86sMHiEI_raw.srt
new file mode 100644
index 0000000000000000000000000000000000000000..0b993f7cae94f9a405b1b5d9e0be05e82dbf245e
--- /dev/null
+++ b/PL9fwy3NUQKwZ1Q7zOg1ObgufPMjRI3WY1/EDL86sMHiEI_raw.srt
@@ -0,0 +1,2568 @@
+1
+00:00:00,490 --> 00:00:04,790
+اليوم ان شاء الله بنحكي في معمل جديد اسمه ال
+
+2
+00:00:04,790 --> 00:00:10,170
+chromatography ال chromatography هي تقنية لفصل
+
+3
+00:00:10,170 --> 00:00:15,630
+الخليط من المواد إلى مكوناتها عندي خليط من المواد
+
+4
+00:00:15,630 --> 00:00:22,170
+من عند المواد لأفصل كل مكون على حدة في البداية
+
+5
+00:00:22,170 --> 00:00:27,150
+نحكي مقدمة عن هذه الطريقة و كيف تم اكتشافهافي
+
+6
+00:00:27,150 --> 00:00:32,530
+عندنا عالم اسمه michael twist هاد عالم روسي استخدم
+
+7
+00:00:32,530 --> 00:00:37,070
+هذه التقنية لفصل أصباغ النباتات وورقة النباتات
+
+8
+00:00:37,070 --> 00:00:41,670
+تحتوي على عدة أنواع من الأصباغ قام في فصل هذه
+
+9
+00:00:41,670 --> 00:00:47,130
+الأصباغ بطريقة أطلق عليها اسم chromatography حكى
+
+10
+00:00:47,130 --> 00:00:50,030
+أن chromatography تعني written color
+
+11
+00:00:50,030 --> 00:00:54,730
+chromatography تعني written color معناها الكتابة
+
+12
+00:00:54,730 --> 00:01:01,040
+بالألوانلأن هو استخدم هذه الطريقة لفصل أصباق
+
+13
+00:01:01,040 --> 00:01:06,320
+النباتات فأطلق عليها إن هي الكتابة بالألوان إنها
+
+14
+00:01:06,320 --> 00:01:11,520
+تستخدم للمواد الملونة لأن هو فصل مواد ملونة فصل
+
+15
+00:01:11,520 --> 00:01:17,730
+أصباقيحكي لإن معظم المواد المحيطة فينا هي حوالينا
+
+16
+00:01:17,730 --> 00:01:22,030
+هي عبارة عن خليط من مادتين أو أكتر most material
+
+17
+00:01:22,030 --> 00:01:24,350
+in our surroundings are a mixture of two or more
+
+18
+00:01:24,350 --> 00:01:28,150
+compounds إن معظم المكونات في الهواء الجوي أو
+
+19
+00:01:28,150 --> 00:01:33,450
+حوالينا في أي مكان هي عبارة عن خليط هذا الخليط إما
+
+20
+00:01:33,450 --> 00:01:38,870
+بيكون homogeneous أو heterogeneousالخليط إما بيكون
+
+21
+00:01:38,870 --> 00:01:43,570
+خليط متجانس homogenous أو خليط غير متجانس
+
+22
+00:01:43,570 --> 00:01:47,990
+heterogeneous إيش يعني homogenous are uniform in
+
+23
+00:01:47,990 --> 00:01:52,110
+composition؟ homogenous معناه خليط متجانس إنه
+
+24
+00:01:52,110 --> 00:01:58,470
+عبارة عن عدة مكونات ولكن يظهر كمادة واحدةزي مثلا
+
+25
+00:01:58,470 --> 00:02:01,630
+الهواء الجو اللي حوالنا يحتوي على عدة أنواع من
+
+26
+00:02:01,630 --> 00:02:06,270
+الغازات لكن هل مبين كل غاز؟ لأ مبين إن هو مكوّن
+
+27
+00:02:06,270 --> 00:02:11,150
+واحد heterogeneous إن ال mixture are not uniform
+
+28
+00:02:11,150 --> 00:02:15,590
+إنه عندنا خليط من عدة مكونات لكن مبين إن هذا يحتوي
+
+29
+00:02:15,590 --> 00:02:20,900
+على أكثر من مادة زي مين؟زي الزيت مع الميه تخلط
+
+30
+00:02:20,900 --> 00:02:24,680
+الزيت مع الميه مبين وبيفصلوا لطبقتين فمبين ان هو
+
+31
+00:02:24,680 --> 00:02:28,400
+عبارة عن اكثر من مادة عبارة عن مادتين واضحين مش
+
+32
+00:02:28,400 --> 00:02:32,460
+متجانسينهي مثال مكتوب أن الـ air هو عبارة عن
+
+33
+00:02:32,460 --> 00:02:35,580
+homogeneous mixture أما ال oil في ال water لما
+
+34
+00:02:35,580 --> 00:02:39,580
+يكون زيت مع مي فهو heterogeneous غير متجانس بيحكي
+
+35
+00:02:39,580 --> 00:02:42,920
+لي سواء الخليط كان homogeneous أو heterogeneous
+
+36
+00:02:42,920 --> 00:02:47,560
+نقدر نفصله نعمل separated by physical method بطرق
+
+37
+00:02:47,560 --> 00:02:51,760
+فيزيائية عدة، إذا سواء الخليط كان عندى متجانس أو
+
+38
+00:02:51,760 --> 00:02:57,000
+غير متجانس بقدر أفصل مكوناته باستخدام عدة طرق في
+
+39
+00:02:57,000 --> 00:03:01,920
+طرق فيزيائيةكيف بدأ اختار الطريقة؟ احنا عندنا عدة
+
+40
+00:03:01,920 --> 00:03:05,120
+طرق للـ separation كيف بدأ اختار الطريقة المناسبة
+
+41
+00:03:05,120 --> 00:03:08,580
+لفصل الخليط بناء على الـtype of mixture على نوع
+
+42
+00:03:08,580 --> 00:03:11,160
+الخليط على الـdifference in the chemical
+
+43
+00:03:11,160 --> 00:03:15,760
+properties على الخصائص الكيميائية لالمكتشرمثلا أنا
+
+44
+00:03:15,760 --> 00:03:21,100
+هختار طريقة الفصل بناء على نوع الخليط أو بناء على
+
+45
+00:03:21,100 --> 00:03:25,340
+الخصائص الكيميائية يعني مثلا من طرق الفصل مثلا
+
+46
+00:03:25,340 --> 00:03:28,280
+عندنا الـ centrifugation الـ centrifugation يمكن
+
+47
+00:03:28,280 --> 00:03:33,110
+أخدته بالمقدمة أن استخدام جهاز centrifugeبيفصل
+
+48
+00:03:33,110 --> 00:03:38,170
+بناءً على الكثافة الدم مثلا نفصله باستخدام هذا
+
+49
+00:03:38,170 --> 00:03:42,310
+الجهاز باستخدام الـ centrifuge مثلا زي ما استخدمنا
+
+50
+00:03:42,310 --> 00:03:46,090
+isolation زي ما عزلنا الكازين من الملك هي هذه
+
+51
+00:03:46,090 --> 00:03:49,310
+طريقة فصل أنه أنا بس أخدت الكازين من كل مكونات
+
+52
+00:03:49,310 --> 00:03:54,150
+الحليب كانت الطريقة اسمها isolationمثلا عملية
+
+53
+00:03:54,150 --> 00:03:58,230
+precipitation أني أرسم مكون وأسيب الباقي وفي عندنا
+
+54
+00:03:58,230 --> 00:04:02,110
+تقنية chromatography وفي عندنا مثلا ال evaporation
+
+55
+00:04:02,110 --> 00:04:06,530
+التبخر أعمل تبخر زي مثلا مي مع ملح ببخر المي و بخل
+
+56
+00:04:06,530 --> 00:04:11,470
+الملح مثلا ال extraction استخلاص أعمل استخلاص
+
+57
+00:04:11,470 --> 00:04:15,570
+للمادة كل هدول طرق لالفصل اليوم احنا الطريقة اللي
+
+58
+00:04:15,570 --> 00:04:20,370
+بدنا ناخدها هي ال chromatographyبحكي للـ
+
+59
+00:04:20,370 --> 00:04:23,930
+chromatography مستخدمة بشكل واسع لفصل ال mixture
+
+60
+00:04:23,930 --> 00:04:29,050
+of compound into individual component لفصل الخليط
+
+61
+00:04:29,050 --> 00:04:33,890
+إلى مكوناته إنه بيخلي كل المادة لحال هذه التقنية
+
+62
+00:04:33,890 --> 00:04:38,630
+مستخدمة بشكل واسع لفصل الخليطاش معنى
+
+63
+00:04:38,630 --> 00:04:41,850
+chromatography؟ chromatography لأنه في البداية
+
+64
+00:04:41,850 --> 00:04:45,750
+استخدموها لفصل الألوان، لأصباغ النباتات، فأطلقوا
+
+65
+00:04:45,750 --> 00:04:50,350
+عليها أنها مستخدمة ل separation of color، لفصل
+
+66
+00:04:50,350 --> 00:04:54,130
+الألوان، ولكن في الوقت الحالي today ال
+
+67
+00:04:54,130 --> 00:04:57,680
+chromatography تعنيفصل ال color و ال colorless
+
+68
+00:04:57,680 --> 00:05:00,940
+substance إنها تستخدم للمواد الملونة و غير
+
+69
+00:05:00,940 --> 00:05:05,180
+الملونة، فإن هي قديما أول ما اكتشفت التقنية كانت
+
+70
+00:05:05,180 --> 00:05:09,240
+فقط بيعتقدوا إنها المواد الملونة، ولكن في اليوم
+
+71
+00:05:09,240 --> 00:05:14,420
+الحاضر هي المواد الملونة و غير الملونةالـ
+
+72
+00:05:14,420 --> 00:05:17,360
+chromatography هي طريقة فيزيائية physical method
+
+73
+00:05:17,360 --> 00:05:21,040
+for separation of mixture to its component
+
+74
+00:05:21,040 --> 00:05:26,820
+لمكوناته بناء على two face باستخدام two face طوران
+
+75
+00:05:26,820 --> 00:05:31,920
+حاجة بنسميها stationary face و حاجة بنسميها ال
+
+76
+00:05:31,920 --> 00:05:37,620
+mobile face إذن هي تقنية فيزيائية أو طريقة
+
+77
+00:05:37,620 --> 00:05:43,220
+فيزيائيةلفصل الخليط إلى مكوناته باستخدام two phase
+
+78
+00:05:43,220 --> 00:05:48,260
+ال mobile phase و ال stationary phase ايش هو ال
+
+79
+00:05:48,260 --> 00:05:51,380
+stationary phase ال stationary phase هو عبارة عن
+
+80
+00:05:51,380 --> 00:05:57,760
+الجزء الصلب بيكون solid مدعم ب glass بجزاز او
+
+81
+00:05:57,760 --> 00:06:02,400
+بتكون عبارة عن paper عن ورقة اذا ال stationary
+
+82
+00:06:02,400 --> 00:06:08,000
+phase هو الطور الساكن هو الطور الساكن بيكون عبارة
+
+83
+00:06:08,000 --> 00:06:12,960
+عنطبعا ورقة ممكن تكون صلبة هي ورقة صلبة تكون مدعمة
+
+84
+00:06:12,960 --> 00:06:17,540
+من الخلف بزجاج أو ألمانيون عشان تحميها من التكسر
+
+85
+00:06:17,540 --> 00:06:22,320
+وممكن تكون عبارة عن بابا خصائصها بتكون برس
+
+86
+00:06:22,320 --> 00:06:27,200
+material هي مادة مثقبة ال stationary phase هي مادة
+
+87
+00:06:27,200 --> 00:06:33,220
+مثقبة برس material ليش؟ عشان تسمح بدخول السائلThe
+
+88
+00:06:33,220 --> 00:06:36,400
+sample is carried by a particular solvent called
+
+89
+00:06:36,400 --> 00:06:40,080
+mobile phase كنت انا كيف تقنيتها دي بيكون في عندي
+
+90
+00:06:40,080 --> 00:06:44,380
+ورقة اسمها stationary phase هذا الطور الساكن هاي
+
+91
+00:06:44,380 --> 00:06:48,240
+بحط عليها العينات الخليط اللي انا بده افصله بحط
+
+92
+00:06:48,240 --> 00:06:50,860
+نقطة من الخليط اللي انا بفصله على ال stationary
+
+93
+00:06:50,860 --> 00:06:54,060
+phase على الطور الساكن في عندي حاجة اسمها ال
+
+94
+00:06:54,060 --> 00:06:57,680
+mobile phase ال mobile phase هو الطور المتحرك
+
+95
+00:06:57,680 --> 00:07:03,070
+بيكون عبارة عن غاز او liquid او ساعلوجدت الورقة
+
+96
+00:07:03,070 --> 00:07:05,750
+الـ stationary phase اللي أنا حطيت عليها الخليط
+
+97
+00:07:05,750 --> 00:07:11,900
+بقى حطها بغمرها في ال mobile phaseبغمر الورقة في
+
+98
+00:07:11,900 --> 00:07:14,840
+ال mobile phase بيبدأ ال mobile phase اللي هو
+
+99
+00:07:14,840 --> 00:07:18,940
+عبارة عن سائل أنه يمر عبر هذه الورقة زي مثلا ال
+
+100
+00:07:18,940 --> 00:07:22,460
+tissue paper لما تحطيها بمي بتتبلل بالمي بيمر المي
+
+101
+00:07:22,460 --> 00:07:25,860
+عبر ال tissue paper و هان هيك ال stationary phase
+
+102
+00:07:25,860 --> 00:07:28,420
+هي الورقة بيكون عليها الخليط أنا حطة نقطة من
+
+103
+00:07:28,420 --> 00:07:31,420
+الخليط عليها و بغمرها في ال mobile phase اللي هو
+
+104
+00:07:31,420 --> 00:07:35,870
+عبارة عن سائل بيمر السائل في هذه الورقةطول ما هو
+
+105
+00:07:35,870 --> 00:07:40,490
+بيمشي السائل بيفصل معاه الخليط لمكوناته على هيئة
+
+106
+00:07:40,490 --> 00:07:44,870
+نقاط كل .. كل مكوّن من هذه المكونات بيعطينا نقطة
+
+107
+00:07:44,870 --> 00:07:48,910
+في مكان معين فالـ Mobile phase هو عبارة عن الـ
+
+108
+00:07:48,910 --> 00:07:53,030
+solvent اسمه ممكن نحكي عنه الـ solvent أو الـluent
+
+109
+00:07:53,030 --> 00:07:58,170
+هو بيكون liquid أو gas الـ mobile phase نبقى
+
+110
+00:07:58,170 --> 00:08:02,770
+الستروزا stationary phaseالستشينري فيس هو جزء
+
+111
+00:08:02,770 --> 00:08:07,450
+الصلب بيكون عبارة عن ورقة ممكن و هي صلب بتكون
+
+112
+00:08:07,450 --> 00:08:11,830
+مدعمة بجلاس أو ألمنيوم ال mobile face عبارة عن
+
+113
+00:08:11,830 --> 00:08:16,030
+solvent اللي you want liquid أو غاز بيكون سائل أو
+
+114
+00:08:16,030 --> 00:08:21,730
+غازبغمر ال stationary phase في ال mobile phase
+
+115
+00:08:21,730 --> 00:08:24,850
+بحكي لي بعدين ال mobile phase بيمر عبر ال
+
+116
+00:08:24,850 --> 00:08:27,810
+stationary phase هيبدأ يمر داخل ال stationary
+
+117
+00:08:27,810 --> 00:08:31,910
+phase الورقة أنا لما أغمرها في السائل بيمشي السائل
+
+118
+00:08:31,910 --> 00:08:35,570
+فيها للأعلى فبيمر ال mobile phase عبر ال
+
+119
+00:08:35,570 --> 00:08:39,630
+stationary phaseطب في عندنا عدة مصطلحات، في عندنا
+
+120
+00:08:39,630 --> 00:08:43,830
+حاجة بنسميها affinity affinity هي المحبة معناها أو
+
+121
+00:08:43,830 --> 00:08:48,090
+التجاذب affinity تعني المحبة أو التجاذب هي
+
+122
+00:08:48,090 --> 00:08:51,030
+attraction or force between .. natural attraction
+
+123
+00:08:51,030 --> 00:08:54,330
+or force between two انسان هو التجاذب الطبيعي
+
+124
+00:08:54,330 --> 00:08:58,950
+للمواد مع بعضها البعض تجاذب الطبيعي للمواد مع
+
+125
+00:08:58,950 --> 00:09:04,030
+بعضها البعضمثلًا المي والسكة هاد بولر و هاد بولر
+
+126
+00:09:04,030 --> 00:09:08,270
+بيدوبوا ببعض يعني هما محببين لبعض فهما نفس الخصائص
+
+127
+00:09:08,270 --> 00:09:12,410
+فبيدوبوا ببعض لذن ال affinity هي التجاذب الطبيعي
+
+128
+00:09:12,410 --> 00:09:17,590
+للمواد مع بعضها البعض adsorption في أن adsorption
+
+129
+00:09:17,590 --> 00:09:22,090
+و absorption adsorption هي adhesion or stickness
+
+130
+00:09:22,090 --> 00:09:24,910
+of substance to the surface of another substance
+
+131
+00:09:24,910 --> 00:09:32,270
+والتصاق مادة على سطح مادة أخرىالتصاق مادة على سطح
+
+132
+00:09:32,270 --> 00:09:36,430
+مادة أخرى التصاق المادة بس على السطح بدون اختراق
+
+133
+00:09:36,430 --> 00:09:40,640
+التركيب الداخلي للمادة هاي ال absorptionabsorption
+
+134
+00:09:40,640 --> 00:09:47,000
+التصاق مادة على سطح مادة أخرى absorption معناها أن
+
+135
+00:09:47,000 --> 00:09:51,580
+المادة تخترق التركيب الداخلي للمادة الأخرى مش
+
+136
+00:09:51,580 --> 00:09:56,880
+التصاق فقط على السطح وإنما اختراق التركيب الداخلي
+
+137
+00:09:56,880 --> 00:10:02,020
+للمادة فيها نوعين رابط يوتيوب هاي بوضح الفرق بين
+
+138
+00:10:02,020 --> 00:10:06,660
+ال absorption و ال absorption إذا حبين تطلعوا عليه
+
+139
+00:10:08,390 --> 00:10:12,550
+هنا توضيح لتقنية ال chromatography نيجي أول شي على
+
+140
+00:10:12,550 --> 00:10:17,150
+اليسار في عندنا هنا الورقة البيضة هذه الورقة هي ال
+
+141
+00:10:17,150 --> 00:10:21,310
+stationary phase لذن الورقة هي الطور الساكن اللي
+
+142
+00:10:21,310 --> 00:10:24,590
+حكينا عنها انها stationary phase بتكون solid بتكون
+
+143
+00:10:24,590 --> 00:10:29,650
+مدعمهلجت؟ الخليط انا وان بده احطه .. المكونات ..
+
+144
+00:10:29,650 --> 00:10:32,690
+عندي خليط عندي كتيوب مليانة فيها خليط من المكونات
+
+145
+00:10:32,690 --> 00:10:36,210
+و بده افصلها باخد منها نقطة بحطها على هذه الورقة
+
+146
+00:10:36,210 --> 00:10:40,540
+على ال stationary phase في موقع مهينتمام؟ و بعدين
+
+147
+00:10:40,540 --> 00:10:44,460
+باخد هذه الورقة اللي صار عليها الخليط بغمرها في
+
+148
+00:10:44,460 --> 00:10:48,560
+داخل ال mobile face ال mobile face هو الجزء الأزرق
+
+149
+00:10:48,560 --> 00:10:51,160
+اللي تحت احنا هالجيت بالصورة اللي على الشمال
+
+150
+00:10:51,160 --> 00:10:54,440
+الورقة البيضة هي ال stationary face التور الساكن
+
+151
+00:10:54,440 --> 00:10:58,100
+حطيت عليها نقطة من هذا الخليط و الخليط كان عبارة
+
+152
+00:10:58,100 --> 00:11:02,820
+عن عدة مكونات هي تحت ملونة في منها أصفر و أحمر و
+
+153
+00:11:02,820 --> 00:11:05,440
+أزرق انها تحتوي على عدة مكونات
+
+154
+00:11:13,230 --> 00:11:17,560
+الجزء الأزرق اللي تحت هو عبارة عن liquid عن سائللا
+
+155
+00:11:17,560 --> 00:11:21,500
+جاتش بدي يسير بدي مر هذا السائل عبر الـ stationary
+
+156
+00:11:21,500 --> 00:11:25,100
+phase بيسير يمشي فيه عبر خاصية الـ capillary
+
+157
+00:11:25,100 --> 00:11:28,600
+action الخاصية الشعرية لأن ال stationary phase
+
+158
+00:11:28,600 --> 00:11:33,120
+حكينا أنها مادة مثقبة porous material فبيمر السائل
+
+159
+00:11:33,120 --> 00:11:37,180
+ال mobile phase عبرها وبيصعد للأعلى وبيظل يمشيطيب
+
+160
+00:11:37,180 --> 00:11:40,100
+كيف ده تفصل المواد طول ما هو بيمشي بيفصل الخليط
+
+161
+00:11:40,100 --> 00:11:44,200
+لعدد مكونات كل واحدة بتوجف في مكان بناء على إيش؟
+
+162
+00:11:44,200 --> 00:11:50,100
+بناء على الـ polarity وبناء على ال solubilityكيف؟
+
+163
+00:11:50,100 --> 00:11:52,440
+إن الورقة اللي أنا بستخدمها ال stationary face
+
+164
+00:11:52,440 --> 00:11:56,420
+بيكون إلها خصائص يعني ممكن تكون polar أو ممكن تكون
+
+165
+00:11:56,420 --> 00:11:59,800
+non polar على اعتبار مثلا ال stationary face هذه
+
+166
+00:11:59,800 --> 00:12:03,120
+اللي استخدمتها هي polar و ال mobile face برضه
+
+167
+00:12:03,120 --> 00:12:06,460
+بيكون إله خصائص إذا ال stationary face كان polar
+
+168
+00:12:06,460 --> 00:12:10,560
+إذا ال mobile face non polarتمام انا بدي اعتبر ال
+
+169
+00:12:10,560 --> 00:12:13,840
+stationary phase هنا ان هو polar و ال mobile phase
+
+170
+00:12:13,840 --> 00:12:17,420
+non polar اجت من اللي بيمشي ال mobile phase هو
+
+171
+00:12:17,420 --> 00:12:21,260
+اللي بيصعد ال stationary phase سابتة مدام ال
+
+172
+00:12:21,260 --> 00:12:25,500
+mobile phase اللي بيصعد هو non polar فالمواد اللي
+
+173
+00:12:25,500 --> 00:12:29,320
+في الخليط إذا كانت non polar هتمشي مع ال mobile
+
+174
+00:12:29,320 --> 00:12:34,740
+phase لفوق تمشي معاه إذا كانت polar هتقف لتحت عند
+
+175
+00:12:34,740 --> 00:12:37,700
+ال stationary phase هتظلها سابتة تحت ومش هتمشي
+
+176
+00:12:38,360 --> 00:12:41,680
+لأنها بتحب ال stationary fast هي خصائصها polar زي
+
+177
+00:12:41,680 --> 00:12:45,300
+ال stationary fast إذا هتوجه فتحت و تدلها مدزجة
+
+178
+00:12:45,300 --> 00:12:48,460
+فيه في ال stationary مش هتمشي مع ال mobile اللي
+
+179
+00:12:48,460 --> 00:12:52,500
+تكون خصائصها زي ال mobile fast مثلا non-polar زيه
+
+180
+00:12:52,500 --> 00:12:56,900
+بتمشي معاه للأعلىففي بعد الفاص اللي بيحكي لي هي
+
+181
+00:12:56,900 --> 00:12:59,560
+separation سهم و حطليها في الصورة التانية اللي على
+
+182
+00:12:59,560 --> 00:13:02,660
+اليمين ماشي ال mobile face السائل الأزرق و طلع
+
+183
+00:13:02,660 --> 00:13:07,700
+الورق لفوق و هو بيمشي بيفصل المكونات هالجت اللي
+
+184
+00:13:07,700 --> 00:13:11,860
+تحت ضاله المربعات أو المستطيلات الزرق هاي معناها
+
+185
+00:13:11,860 --> 00:13:14,980
+مدامها ضالت لتحت ملزقة إذا ان هي بتحب ال
+
+186
+00:13:14,980 --> 00:13:20,640
+stationary face هي محببة خصائصها نفس خصائص ال
+
+187
+00:13:20,640 --> 00:13:25,060
+stationary faceاللي بتضلها ملزقة تحت خصائصها نفس
+
+188
+00:13:25,060 --> 00:13:27,740
+خصائص ال stationary phase يعني إذا ال stationary
+
+189
+00:13:27,740 --> 00:13:31,680
+polar إذا هي polar زيه مابتحبش ال mobile فماطلعتش
+
+190
+00:13:31,680 --> 00:13:36,100
+معاه اللي بعدها بتكون أقل polarity اللي بعدها أقل
+
+191
+00:13:36,100 --> 00:13:40,480
+اللي بتطلع للآخر إذا هي نفس خصائص ال mobile phase
+
+192
+00:13:40,480 --> 00:13:46,010
+فطلعت معاه للآخريعني ال affinity المحبة للي تحت
+
+193
+00:13:46,010 --> 00:13:49,230
+دلت بنسجة تحت تكون ال affinity عالية مع ال
+
+194
+00:13:49,230 --> 00:13:52,790
+stationary face اللي فوق الأصفر الدوائر الأصفر
+
+195
+00:13:52,790 --> 00:13:56,050
+مدامها طلعت مع ال mobile face وطلعت للآخر إذا إن
+
+196
+00:13:56,050 --> 00:14:01,300
+هي ال affinity عالية مع ال mobile faceالـ
+
+197
+00:14:01,300 --> 00:14:04,340
+chromatography technique ايش عملتلي؟ عملتلي
+
+198
+00:14:04,340 --> 00:14:07,980
+analyze عملتلي تحليل حللتلي ال mixture لها عدة
+
+199
+00:14:07,980 --> 00:14:12,260
+مكونات عملتلي identify انه عرفتني هذا ال mixture
+
+200
+00:14:12,260 --> 00:14:18,260
+شو مكوناته purify عملتلي تنقية بدل ما كان خليط من
+
+201
+00:14:18,260 --> 00:14:23,930
+عدة مكونات صار كل مكون لحالعملت لي quantify كمي
+
+202
+00:14:23,930 --> 00:14:28,010
+أنه أنا عرفت هذا الخليط مكون من أكم من مادة يحتوي
+
+203
+00:14:28,010 --> 00:14:32,130
+على أكم من مادة يعني هو فصل لعدة مواد كل واحد في
+
+204
+00:14:32,130 --> 00:14:36,910
+منطقة مثلا الأصفر هي مادة أحمر مادتين ودوائر
+
+205
+00:14:36,910 --> 00:14:40,930
+السودة هي تلت مواد والمستطيلات هي أربع مواد إذا
+
+206
+00:14:40,930 --> 00:14:46,820
+هذا الخليط يتكون من أربع موادهنا التفصيل للي
+
+207
+00:14:46,820 --> 00:14:51,060
+توحكناه كله تقنية الـ separation is based on fact
+
+208
+00:14:51,060 --> 00:14:54,280
+that برص solid absorbed different substance to
+
+209
+00:14:54,280 --> 00:14:57,720
+different extreme depend on polarity أنه عندنا
+
+210
+00:14:57,720 --> 00:15:01,620
+مادة بتكون برص اللي هي عبارة عن stationary phase
+
+211
+00:15:02,220 --> 00:15:07,360
+تكون بورص material مادة مثقبة أنا هحط عليها القليل
+
+212
+00:15:07,360 --> 00:15:11,360
+اللي انا بده افصله وهيتم الفصل او انها تلتصق في
+
+213
+00:15:11,360 --> 00:15:14,280
+هذه ال stationary phase المكون اذا لتصق في ال
+
+214
+00:15:14,280 --> 00:15:17,320
+stationary phase او انه يمشي مع ال mobile phase
+
+215
+00:15:17,320 --> 00:15:21,440
+ومايلزجش بناء على ايش؟ بناء على ال polarity على
+
+216
+00:15:21,440 --> 00:15:25,200
+القطبية اذا ال stationary polar اذا المواد ال
+
+217
+00:15:25,200 --> 00:15:28,260
+polar هتضلهم لزجة فيه والمواد ال non polar هتطلع
+
+218
+00:15:28,260 --> 00:15:32,630
+لفوقإذا الـ stationary non-polar إذا المواد الـ
+
+219
+00:15:32,630 --> 00:15:38,060
+non-polar بتضالهم لزجة فيه و الـ polar بيطلع لفاهa
+
+220
+00:15:38,060 --> 00:15:40,980
+mixture to be separated is first applied to an
+
+221
+00:15:40,980 --> 00:15:44,440
+immovable برة solid إذا المكتشر الخليط اللي أنا
+
+222
+00:15:44,440 --> 00:15:47,540
+بده أفصله وين بحطه أول إشي بالبداية بحطه على ال
+
+223
+00:15:47,540 --> 00:15:51,080
+stationary ولا على ال mobile لأ بأسقطه في ال
+
+224
+00:15:51,080 --> 00:15:54,200
+stationary phase في البرة ال solid material اللي
+
+225
+00:15:54,200 --> 00:15:57,720
+هي المادة المثقبة اللي بتكون عبارة عن ورقة حكاية
+
+226
+00:15:57,720 --> 00:16:02,880
+أو ألمنيوم أو سليكا اللي هي اسمها stationary phase
+
+227
+00:16:03,510 --> 00:16:06,430
+the component of the mixture then get washed
+
+228
+00:16:06,430 --> 00:16:10,610
+وبعدين بغمره حكاية البر السايماتيريال اللي هي ال
+
+229
+00:16:10,610 --> 00:16:14,350
+stationary phase بغمرها في ال solvent اللي بنسميه
+
+230
+00:16:14,350 --> 00:16:18,990
+mobile phase بعدين ال mixture بيصيرله separated by
+
+231
+00:16:18,990 --> 00:16:22,830
+different بناء على إيش؟ بناء على ال attraction،
+
+232
+00:16:22,830 --> 00:16:26,850
+بناء على ال polarity مع ال stationary phase و ال
+
+233
+00:16:26,850 --> 00:16:30,970
+mobile phase، بناء على أي مبدأ، بناء على مبدأ
+
+234
+00:16:30,970 --> 00:16:34,960
+like، dissolve likeلذن بعد ما أحط المواد على ال
+
+235
+00:16:34,960 --> 00:16:38,620
+stationary phase بغمرها في ال mobile phase بيبدأ
+
+236
+00:16:38,620 --> 00:16:41,420
+يصعد ال mobile phase عبر ال stationary phase
+
+237
+00:16:41,420 --> 00:16:46,340
+وهيمشي معاه مواد هتمشي معاه مكونات الخليط بناء على
+
+238
+00:16:46,340 --> 00:16:50,220
+إيش، بناء على ال polarity، بناء على ال affinity،
+
+239
+00:16:50,220 --> 00:16:54,830
+بناء على ال attractionالتجادب بينها وبين الـ Face
+
+240
+00:16:54,830 --> 00:16:58,930
+إذا هي والله متحبة و Affinity وقوة تجادب عالية
+
+241
+00:16:58,930 --> 00:17:02,590
+وخصائصها الـ Polarity نفس خصائص الـ Stationary إذا
+
+242
+00:17:02,590 --> 00:17:05,970
+هتظلها ملزجة في الـ Stationary مش هتصعد للأعلى إذا
+
+243
+00:17:05,970 --> 00:17:09,690
+خصائصها و الـ Affinity و التجادب قوي مع الـ Mobile
+
+244
+00:17:09,690 --> 00:17:16,270
+Face إذا هي هتصعد مع الـ Mobile Face للأعلىif the
+
+245
+00:17:16,270 --> 00:17:19,330
+stationary phase is polar إذا الـ stationary كانت
+
+246
+00:17:19,330 --> 00:17:22,030
+polar إذا أن الـ polar component المكونات اللي
+
+247
+00:17:22,030 --> 00:17:27,740
+بتكون موجودة في الخليط كانت polarهي هتلزج في ال
+
+248
+00:17:27,740 --> 00:17:32,060
+stationary phase مش هتطلع لفوق و ال non-polar هي
+
+249
+00:17:32,060 --> 00:17:36,260
+اللي هتطلع أسرع للأعلى لكن لو ال stationary phase
+
+250
+00:17:36,260 --> 00:17:40,180
+is non-polar إذا ال non-polar هي اللي هتتجادب مع
+
+251
+00:17:40,180 --> 00:17:43,840
+ال stationary و تضلها تحت و ال polar هو اللي هيصعد
+
+252
+00:17:43,840 --> 00:17:50,860
+للأعلى بقوة أعلىأو بواجهة أقل الـ separation
+
+253
+00:17:50,860 --> 00:17:54,680
+depends on several factors عملية الفصل بتعتمد على
+
+254
+00:17:54,680 --> 00:17:59,640
+عدة عوامل عملية الفصل بتعتمد على عدة عوامل في
+
+255
+00:17:59,640 --> 00:18:03,620
+عندنا عاملين رئيسيين وفي عندنا التالت أول عامل هي
+
+256
+00:18:03,620 --> 00:18:10,060
+solubility solubility with mobile faceأول عامل الـ
+
+257
+00:18:10,060 --> 00:18:13,400
+solubility with mobile face اللي مكتوب هنا الـ
+
+258
+00:18:13,400 --> 00:18:16,460
+solvent الـ solvent معناه ال mobile face إذا كان
+
+259
+00:18:16,460 --> 00:18:19,780
+بيدوب في ال mobile face له نفس خصائص ال mobile
+
+260
+00:18:19,780 --> 00:18:24,520
+face إذا هو هيصعد للأعلى العامل التاني ال
+
+261
+00:18:24,520 --> 00:18:29,350
+attractionbetween compound and silica اللي هي قوة
+
+262
+00:18:29,350 --> 00:18:32,570
+التجاذب بين المادة اللي موجودة في الخليط والسيليكا
+
+263
+00:18:32,570 --> 00:18:35,450
+اللي هي ال stationary phase ال attraction يعني
+
+264
+00:18:35,450 --> 00:18:39,070
+التجاذب مع ال stationary phase إذا هي متجاذبة أو
+
+265
+00:18:39,070 --> 00:18:44,410
+حابة لل stationary إذا هتظلها في الأسفل إذا أول
+
+266
+00:18:44,410 --> 00:18:47,550
+عاملين رئيسيا يعتمد عليهم تقنية الفصل هي
+
+267
+00:18:47,550 --> 00:18:51,920
+solubility with mobile phaseوالـ Attraction with
+
+268
+00:18:51,920 --> 00:18:56,120
+stationary face وهذا هو نفسه الـ principle لما
+
+269
+00:18:56,120 --> 00:19:00,100
+أعمل اليوم أنه أنا بدي أعمل فصل لخريطة من مكونات
+
+270
+00:19:00,100 --> 00:19:03,920
+من عدة مكونات بناء على أيش بده يفصل، بناء على أي
+
+271
+00:19:03,920 --> 00:19:07,900
+مبدأ، بناء على مبدأ ال solubility with mobile face
+
+272
+00:19:07,900 --> 00:19:12,760
+أنه مدى الذائبية مع ال mobile face و ال attraction
+
+273
+00:19:12,760 --> 00:19:16,820
+مع الstationary phase ومدى التجاذب مع الـ
+
+274
+00:19:16,820 --> 00:19:19,380
+stationary phase والله إذا هي متجاذبة مع ال
+
+275
+00:19:19,380 --> 00:19:22,780
+stationary phase هتضلها لتحت ومش هتطلع إذا هي
+
+276
+00:19:22,780 --> 00:19:25,100
+solubility مع ال mobile phase اللي هي عالية
+
+277
+00:19:25,100 --> 00:19:29,280
+ويزائبة في ال mobile phase إذا هتصعد للأعلى تالت
+
+278
+00:19:29,280 --> 00:19:34,310
+عامل هو ال structure of compound ليه الحجم؟التالت
+
+279
+00:19:34,310 --> 00:19:38,570
+عامل يقصد فيه ال size حجم المادة اللى موجودة في
+
+280
+00:19:38,570 --> 00:19:42,930
+الخليط اللى بدي تمفصلها إذا حجمها كبير هتمشي بشواش
+
+281
+00:19:42,930 --> 00:19:47,830
+هتكون بطيقة في المشي وبالتالي مش هتمشي كتير هتكون
+
+282
+00:19:47,830 --> 00:19:53,330
+ملزقة لتحت أكتر لكن إذا كان حجمها صغير هتمشي أسرع
+
+283
+00:19:55,830 --> 00:20:00,770
+إيش مميزات ال chromatography ال advantage of
+
+284
+00:20:00,770 --> 00:20:04,650
+chromatography أول حاجة كان separate complex
+
+285
+00:20:04,650 --> 00:20:08,570
+mixture with greater precision أنها بتفصل خليط من
+
+286
+00:20:08,570 --> 00:20:14,490
+عدة مكونات بدقة عالية بتفصل خليط بدقة عالية يعني
+
+287
+00:20:14,490 --> 00:20:20,600
+لو في مادتين متشابهاتولكن بيختلفوا زي البروتين
+
+288
+00:20:20,600 --> 00:20:23,540
+مثلا هو عبارة عن سلسلة من أحمض أمينية لو في عندي
+
+289
+00:20:23,540 --> 00:20:27,320
+نوعين من البروتين هما نفس سلسلة أحمض الأمينية بس
+
+290
+00:20:27,320 --> 00:20:31,700
+بيختلفوا بحمض أميني واحد بتقدر تفصلوا هذه التقنية
+
+291
+00:20:31,700 --> 00:20:36,350
+تفصلهم عن بعضCan purify any soluble or volatile
+
+292
+00:20:36,350 --> 00:20:39,270
+substance if right adsorbent material, carrier
+
+293
+00:20:39,270 --> 00:20:42,930
+fluid and operating condition are employed بتقدر
+
+294
+00:20:42,930 --> 00:20:49,930
+تعملي تنقية لأي خليط من أي مواد، من عدة مواد تقدر
+
+295
+00:20:49,930 --> 00:20:53,930
+تنقي الخليط، أنه تخلي كل مكوّن لحال، بشرط أن
+
+296
+00:20:53,930 --> 00:20:57,630
+أستخدم ال stationary phase المناسب، ال mobile
+
+297
+00:20:57,630 --> 00:21:01,930
+phase المناسب والتقنيات و الخطوات إجراء التجربة
+
+298
+00:21:01,930 --> 00:21:07,280
+الصحيحةexact quantitative analysis is done even
+
+299
+00:21:07,280 --> 00:21:11,240
+from a trace compound بقدر أعمل منها quantitative
+
+300
+00:21:11,240 --> 00:21:16,560
+analysis اللي هو تحليل كمي أعرف كمية اربعة small
+
+301
+00:21:16,560 --> 00:21:19,780
+material consumption اللي هو هذه التقنية بتستهلكش
+
+302
+00:21:19,780 --> 00:21:24,920
+مواد كتيرة، مواد بسيطة ال quantization has a broad
+
+303
+00:21:24,920 --> 00:21:30,200
+linearity range الها مدى واسع، الها مدى واسع من
+
+304
+00:21:30,200 --> 00:21:35,680
+المواد اللي تقدر تفصلهاستة analyze of several
+
+305
+00:21:35,680 --> 00:21:39,720
+compounds can be done during one run بقدر أفصل
+
+306
+00:21:39,720 --> 00:21:43,280
+أكتر من خليط مرة واحدة يعني بجيب ال stationary
+
+307
+00:21:43,280 --> 00:21:47,100
+phase اللي بده أحط عليه الخليط بقى بحط خليط في
+
+308
+00:21:47,100 --> 00:21:50,580
+مكان بحط نقطة من هذا الخليط في مكان تاني نقطة من
+
+309
+00:21:50,580 --> 00:21:55,420
+الخليط التاني في نفس الورقة بقدر أفصل أدت خليط أدت
+
+310
+00:21:55,420 --> 00:21:59,840
+أنواع من الخليط ممكن خليط اتنين تلاتة في نفس ال
+
+311
+00:21:59,840 --> 00:22:05,130
+stationary phase في نفس التقنية في نفس المرةالـ
+
+312
+00:22:05,130 --> 00:22:07,930
+chromatography is a fast analysis method هي طريقة
+
+313
+00:22:07,930 --> 00:22:12,650
+سريعة و آخر إشي أنه حالياً صارت instrumentation
+
+314
+00:22:12,650 --> 00:22:15,910
+صارت عبارة عن أجهزة صارت automation بدل ما نعملها
+
+315
+00:22:15,910 --> 00:22:20,190
+بشكل يدوي اتم تقنية الفصل باستخدام أجهزة
+
+316
+00:22:22,690 --> 00:22:26,430
+في عندنا سؤال is this a pure substance or mixture؟
+
+317
+00:22:26,430 --> 00:22:31,270
+انه هل المادة اللي عندي هي مادة عبارة عن مادة نقية
+
+318
+00:22:31,270 --> 00:22:34,890
+بتتكون من مكون واحد ولا هي عبارة عن خليط كيف
+
+319
+00:22:34,890 --> 00:22:39,170
+بتعرف؟ انا أعطيتك مادة وحكيتلك اعرفيها هل هي pure
+
+320
+00:22:39,170 --> 00:22:43,090
+substance ولا mixture؟كيف بدك تعرفي؟ بيحكيلي
+
+321
+00:22:43,090 --> 00:22:46,830
+بنشتغل تقنية لـ chromatography بشكل عام، هذا ملخص
+
+322
+00:22:46,830 --> 00:22:49,670
+للـ slide هاي أن أنا بدي أشتغل تقنية لـ
+
+323
+00:22:49,670 --> 00:22:54,030
+chromatography هنكتب للـ TLC TLC هو نوع من أنواع
+
+324
+00:22:54,030 --> 00:22:58,790
+الكروماتوجرافي هنتعرف عليه لقدام هي اختصارها thin
+
+325
+00:22:58,790 --> 00:23:04,560
+layer chromatographyTLC Thin Layer Chromatography
+
+326
+00:23:04,560 --> 00:23:07,800
+بشكل عام انا كيف بده اعرف بده اجاوب عليها ده
+
+327
+00:23:07,800 --> 00:23:12,140
+السؤال هل هي pure مادة نقية ولا خليط بدي اشتغل
+
+328
+00:23:12,140 --> 00:23:16,820
+تقنية ال chromatographyوأشتغلها بعد ما يفصل الخليط
+
+329
+00:23:16,820 --> 00:23:19,520
+أو يمشي ال mobile phase عبر ال stationary phase
+
+330
+00:23:19,520 --> 00:23:24,300
+إذا أعطاني نقطة واحدة أنه في مكان واحد نقطة واحدة
+
+331
+00:23:24,300 --> 00:23:28,520
+إذا هذه pure substance مادة نقية لأنه ماعطانيهش
+
+332
+00:23:28,520 --> 00:23:33,380
+غير مكون واحد إذا أعطاني أكتر من نقطة في أكتر من
+
+333
+00:23:33,380 --> 00:23:38,610
+مكان فهذا معناه أنه كان mixture كان عبارةعن خليط
+
+334
+00:23:38,610 --> 00:23:43,370
+وفصل إلى عدة مكونات وبالتالي أنا بقدر أعرف ال
+
+335
+00:23:43,370 --> 00:23:47,030
+number of components in a mixture بقدر أعرف عدد
+
+336
+00:23:47,030 --> 00:23:50,850
+مكونات الخليط كيف بده يعرف عدد مكونات الخليط؟ لإنه
+
+337
+00:23:50,850 --> 00:23:55,290
+بعد ما يفصل أكم نقطة أعطاني مثلا إذا أعطاني تلاتة
+
+338
+00:23:55,290 --> 00:23:58,970
+إذا هو عبارة عن تلات مكونات إذا أعطاني بس نقطين
+
+339
+00:23:58,970 --> 00:24:04,420
+إذا هو عبارة عن مكوناتطيب are these compounds
+
+340
+00:24:04,420 --> 00:24:07,940
+identical؟ السؤال التاني انه بيحكيلي ان انا اعرف
+
+341
+00:24:07,940 --> 00:24:15,300
+هل هدول المواد identical شبه بعض انه عندك مادتين و
+
+342
+00:24:15,300 --> 00:24:19,840
+مش عارفين هل هم نفس المادة محطوطين في مكانين ولا
+
+343
+00:24:19,840 --> 00:24:23,700
+هم مادتين مختلفات كيف بدك تعرفي؟ برضه بيحكيلي
+
+344
+00:24:23,700 --> 00:24:26,900
+الإجابة اني بدي اشتغل ال chromatographic technique
+
+345
+00:24:26,900 --> 00:24:32,930
+بدي اشتغل تقنية ال chromatographyباخد من المادة
+
+346
+00:24:32,930 --> 00:24:35,750
+الأولى و بحطها على ال stationery face و المادة
+
+347
+00:24:35,750 --> 00:24:38,470
+التانية و بحطها برضه على الجانب التاني في نفس
+
+348
+00:24:38,470 --> 00:24:41,810
+الورقة كل واحدة في مكان و بحطها في ال mobile face
+
+349
+00:24:41,810 --> 00:24:45,730
+بغمرها و بيبدأ يفصل بحكي لي إذا فصلوا النقطتين
+
+350
+00:24:45,730 --> 00:24:50,130
+مقابل بعض في نفس المكان مقابل بعض إذا هم نفس
+
+351
+00:24:50,130 --> 00:24:55,230
+المادة إذا فصلوا كل واحدة في مكان إذا هم مادتين
+
+352
+00:24:55,230 --> 00:25:01,190
+مختلفةإذا أنا هشتغل التنتين و أعملهم فصل إذا تم
+
+353
+00:25:01,190 --> 00:25:08,390
+الفصل في نفس المكان إذا هو عبارة عن identical
+
+354
+00:25:08,390 --> 00:25:12,710
+substance أما إذا فصلوا كل واحدة في مكان بيكونوا
+
+355
+00:25:12,710 --> 00:25:17,990
+مواد مختلفة وكيف كمان بدي أعرف الزيادة؟ نحسب حاجة
+
+356
+00:25:18,110 --> 00:25:22,510
+الـ retention factor هنتعرف عليه لقدام لكن أنا
+
+357
+00:25:22,510 --> 00:25:25,970
+بشكل عام كيف بدأ أعرف هل هي identical ولا لأ نفس
+
+358
+00:25:25,970 --> 00:25:30,770
+المادة أشتغل تقنية ال chromatography إذا فصل
+
+359
+00:25:30,770 --> 00:25:36,050
+المكونين إجبال بعض في نفس المكان على الورقة مقابل
+
+360
+00:25:36,050 --> 00:25:40,170
+بعض إذا هم نفس المادة إذا كل واحدة في مكان إذا هم
+
+361
+00:25:40,170 --> 00:25:47,310
+مادتين مختلفةtype of chromatography أقسام لـ
+
+362
+00:25:47,310 --> 00:25:50,230
+chromatography لجهة ال chromatography مقسم إلى
+
+363
+00:25:50,230 --> 00:25:54,690
+قسمين بناء على ال stationary و بناء على ال mobile
+
+364
+00:25:54,690 --> 00:25:59,110
+phase ال chromatography مقسم إلى جزئين بناء على ال
+
+365
+00:25:59,110 --> 00:26:02,190
+stationary إليه تقسيمة و بناء على ال mobile إليه
+
+366
+00:26:02,190 --> 00:26:05,670
+تقسيمة بناء على ال mobile phase فيه نوعين إما
+
+367
+00:26:05,670 --> 00:26:09,250
+بستخدم ال mobile phase يكون gas، غاز أو بكون
+
+368
+00:26:09,250 --> 00:26:13,230
+liquidبناء على ال stationary face في منه column
+
+369
+00:26:13,230 --> 00:26:17,510
+على شكل عمود و في منه planner على شكل سطح مستوي
+
+370
+00:26:17,510 --> 00:26:21,130
+اذا بناء على ال stationary face في منه اتنين
+
+371
+00:26:21,130 --> 00:26:25,570
+column عمود او planner مستوي و ال planner في منه
+
+372
+00:26:25,570 --> 00:26:30,150
+thin layer chromatography و في منه paper
+
+373
+00:26:30,150 --> 00:26:36,110
+chromatographyهنا جدول بيوضح انه او انه ملخص هاي
+
+374
+00:26:36,110 --> 00:26:38,790
+ال slide المطلوب منها اصلا منها انه ال
+
+375
+00:26:38,790 --> 00:26:41,890
+chromatography تقسيم ال chromatography بناء على ال
+
+376
+00:26:41,890 --> 00:26:45,510
+mobile phase ان في عندنا liquid chromatography و
+
+377
+00:26:45,510 --> 00:26:49,610
+gas chromatography بس لذا تقسيم ال chromatography
+
+378
+00:26:49,610 --> 00:26:53,010
+بناء على ال mobile phase في منها liquid و في منها
+
+379
+00:26:53,010 --> 00:26:57,780
+gasالـ classification أما بناءً عن ال stationary
+
+380
+00:26:57,780 --> 00:27:00,740
+phase بناءً عن ال stationary phase حكينا في منها
+
+381
+00:27:00,740 --> 00:27:05,100
+planner مستوي و في منها column عمودي ال planner ال
+
+382
+00:27:05,100 --> 00:27:08,260
+chromatography بيحكي إنها separation technique
+
+383
+00:27:08,260 --> 00:27:11,920
+which ال stationary phase بيكون عبارة عن إبلان هي
+
+384
+00:27:11,920 --> 00:27:15,420
+تقنية فصلبيكون فيها الستاشينة اللي فيها سطور
+
+385
+00:27:15,420 --> 00:27:19,740
+التابت عبارة عن plan عبارة عن سطح مستول هذا سطح
+
+386
+00:27:19,740 --> 00:27:25,820
+المستول plan إما بيكون بابر ورقة أو بيكون جزيئات
+
+387
+00:27:25,820 --> 00:27:30,340
+صلبة زي السيليكا أو cellulose أو aluminium oxide
+
+388
+00:27:30,340 --> 00:27:35,720
+أو ion exchange resin ومدعمة من الخلف بجزاز أو
+
+389
+00:27:35,720 --> 00:27:41,560
+aluminiumإذا الـ plan chromatography planar
+
+390
+00:27:41,560 --> 00:27:44,960
+chromatography تقنية لفصل المواد بيكون فيها
+
+391
+00:27:44,960 --> 00:27:49,220
+الستشرينا رفاس عبارة عن plan عبارة عن سطح مستوي و
+
+392
+00:27:49,220 --> 00:27:52,720
+ال plan had ممكن يكون على شكلين ممكن يكون بابر و
+
+393
+00:27:52,720 --> 00:27:58,180
+ورقة الستشرينا رفاس عبارة عن بابر أو بيكون عبارة
+
+394
+00:27:58,180 --> 00:28:05,900
+عن جزء صلب مدعم بجزاز أو ألمنيومعشان بيكون الجزء
+
+395
+00:28:05,900 --> 00:28:10,660
+من الأمام بتكسر بسهولة، بيكون لين، فبيكون مدعم من
+
+396
+00:28:10,660 --> 00:28:15,040
+الخلف بجزء من الـ glass أو الالمنيوم فمنها نوعين
+
+397
+00:28:15,040 --> 00:28:18,760
+البلانار إما thin layer chromatography أو بابا الـ
+
+398
+00:28:18,760 --> 00:28:21,480
+thin layer هي اللي حكينا عنها اللي بتكون عبارة عن
+
+399
+00:28:21,480 --> 00:28:25,120
+.. لسه شينري فاس عبارة عن طبقة رفيعة، بتكون thin
+
+400
+00:28:25,120 --> 00:28:29,330
+layer، طبقة رفيعةفعشانها رفيعة بتكون سهلة التكسر
+
+401
+00:28:29,330 --> 00:28:34,650
+فهي مدعمة سبارتت بجلاز plastic أو aluminium بجزاز
+
+402
+00:28:34,650 --> 00:28:38,690
+أو plastic أو aluminium ال paper chromatography
+
+403
+00:28:38,690 --> 00:28:41,930
+paper chromatography ال stationary face بيكون
+
+404
+00:28:41,930 --> 00:28:45,570
+عبارة عن paper عبارة عن ورقة أما الجزء التاني هو
+
+405
+00:28:45,570 --> 00:28:49,310
+ال column chromatography اللي بيكون عبارة عن عمود
+
+406
+00:28:49,310 --> 00:28:54,860
+فال stationary face بيكون columnطيب ليش بنستخدم
+
+407
+00:28:54,860 --> 00:28:57,480
+تقنية ال chromatography؟ بيحكينا ال
+
+408
+00:28:57,480 --> 00:29:00,580
+chromatographic technique ممكن نستخدمها ك
+
+409
+00:29:00,580 --> 00:29:06,820
+qualitative و ك quantitative ممكن استخدمها ك
+
+410
+00:29:06,820 --> 00:29:11,480
+qualitative و quantitative ايش يعني qualitative؟
+
+411
+00:29:11,480 --> 00:29:14,740
+يعني اعرف نوع المادة، اعرف اسم المادة، مين هذه
+
+412
+00:29:14,740 --> 00:29:20,370
+المادةQualitative معناها جودة أو نوع المادة تحديد
+
+413
+00:29:20,370 --> 00:29:24,610
+نوع المادة كيبدا حدد نوع المادة بناء على حاجة
+
+414
+00:29:24,610 --> 00:29:29,450
+اسمها ال retention factor انه انا بحط خليط من
+
+415
+00:29:29,450 --> 00:29:32,990
+المواد على ال stationary face و بحطها بغمرها بال
+
+416
+00:29:32,990 --> 00:29:37,650
+mobile face و بيبدأ يفصل بيفصل في كل مكون إلى نقاط
+
+417
+00:29:37,650 --> 00:29:43,170
+كل نقطة في مكان اجيت وين وصلت هذه النقطة؟من عندها
+
+418
+00:29:43,170 --> 00:29:47,670
+بحسب حاجة لكل نقطة بحسب إيش اسمه retention factor
+
+419
+00:29:48,850 --> 00:29:53,310
+اللي هو عامل المحافظة كيف بيانحسب هاد؟ هو موجود في
+
+420
+00:29:53,310 --> 00:29:56,410
+آخر slide بس أنه بشكل عام ال retention factor هو
+
+421
+00:29:56,410 --> 00:30:00,290
+عبارة عن ال distance اللي هي المسافة اللي قطعتها
+
+422
+00:30:00,290 --> 00:30:04,470
+المادة، بكون أنا حاطة خط بداية اللي هو حاطيت من
+
+423
+00:30:04,470 --> 00:30:07,190
+عنده الخليط، النقطة وين أنت حاطيت الخليط، من وين
+
+424
+00:30:07,190 --> 00:30:10,970
+بدأتي، من هنا أنا حاطيت النقطة، بحسب المسافة اللي
+
+425
+00:30:10,970 --> 00:30:13,930
+قطعتها المادة من هذا الخط لوين وصلت بعد الفصل
+
+426
+00:30:14,870 --> 00:30:19,730
+المادة وين وصلت بعد الفصل يعني لو رجعنا لهذه
+
+427
+00:30:19,730 --> 00:30:25,790
+الصورة هل جت المواد بعد الفصل مثلا الحمرة انت حطيت
+
+428
+00:30:25,790 --> 00:30:29,350
+الخليط وين؟ من تحت من خط البداية من تحت صح؟ بدي
+
+429
+00:30:29,350 --> 00:30:32,330
+أحسب المسافة من خط البداية اللي عند الحمرة بدي
+
+430
+00:30:32,330 --> 00:30:37,580
+أجيس بالمسطرة جدش قطعت الحمرة مسافةتمام؟ على
+
+431
+00:30:37,580 --> 00:30:41,640
+المسافة اللى قطحها الـ mobile phase من البداية
+
+432
+00:30:41,640 --> 00:30:45,300
+لوين وصل يعني الجزء الأزرق السائل الأزرق هاد من
+
+433
+00:30:45,300 --> 00:30:50,160
+وين كان من البداية لفوق لجزء الأزرق لآخر وين وصل
+
+434
+00:30:50,160 --> 00:30:53,220
+هذه المسافة كلها برضه بده جثة في المسطرة ال
+
+435
+00:30:53,220 --> 00:30:56,340
+retention factor هو عبارة عن المسافة اللى قطعتها
+
+436
+00:30:56,340 --> 00:31:00,760
+المادة من خط البداية على المسافة اللى قطحها ال
+
+437
+00:31:00,760 --> 00:31:05,370
+solvent كامل من خط البداية لعند ما وصلهذه اسمها
+
+438
+00:31:05,370 --> 00:31:09,010
+retention factor إذاً ال retention factor هي
+
+439
+00:31:09,010 --> 00:31:15,850
+المسافة اللي قطعتها المادة أعلى المسافة اللي قطعها
+
+440
+00:31:15,850 --> 00:31:19,190
+ال solvent من خط البداية إلى وين وصل كده بيطلع
+
+441
+00:31:19,190 --> 00:31:23,270
+عندي رقم كل مادة إلها retention factor معروف
+
+442
+00:31:23,270 --> 00:31:27,350
+ومحفوظ في جدول وجدول علماء ومكتوبة في جدول برجع
+
+443
+00:31:27,350 --> 00:31:31,250
+لهذا الجدول بدور على الرقم اللي طلع معاهretention
+
+444
+00:31:31,250 --> 00:31:34,970
+factor اللي طلعت معاه بطلع بروح بدور في الجدول
+
+445
+00:31:34,970 --> 00:31:39,050
+مثلا ستة بروح أشوف قبل الستة مين هي المادة بس بطلع
+
+446
+00:31:39,050 --> 00:31:43,730
+أسمها و بكون عارفيتها، بعرفها مثلا نص، بروح للجدول
+
+447
+00:31:43,730 --> 00:31:47,810
+بطلع مين النص؟ مثلا لل glycine طلعت النص، إذا
+
+448
+00:31:47,810 --> 00:31:51,390
+المادة هي عبارة عن glycine ف qualitative analysis
+
+449
+00:31:51,390 --> 00:31:54,950
+كيف؟ من خلال أني أحسب ال retention factor
+
+450
+00:31:56,250 --> 00:31:59,370
+Quantitative determination برضه بيستخدم ك
+
+451
+00:31:59,370 --> 00:32:04,050
+quantitative quantitative يعني كمية يعني بتعرف
+
+452
+00:32:04,050 --> 00:32:08,390
+كمية المادة الموجودة جداش كميتها بيحكيلي هذا بيتم
+
+453
+00:32:08,390 --> 00:32:12,590
+معرفته بناء على ال intensity of a spot وبناء على
+
+454
+00:32:12,590 --> 00:32:17,110
+ال side of the spot بناء على شدة ال only spot هي
+
+455
+00:32:17,110 --> 00:32:20,110
+اللي جيت بعد ما يفصل المواد وبيعطينيها على شكل
+
+456
+00:32:20,110 --> 00:32:25,010
+نقاط spot بنسميها نقاطهذه النقطة جدّيش حجمها
+
+457
+00:32:25,010 --> 00:32:29,050
+الدائرة اللي طلعت معي جدّيش حجمها اللي هي صغيرة
+
+458
+00:32:29,050 --> 00:32:33,750
+ولا كبيرة طب الشد تعدها واضحة، نغامجة ولا إنها
+
+459
+00:32:33,750 --> 00:32:38,710
+خفيفة؟ هذه بتحدد لكمية المادة إذا كان مثلا حجمها
+
+460
+00:32:38,710 --> 00:32:43,110
+كبير إذا هي مادة موجودة بكمية كبيرة إذا حجمها صغير
+
+461
+00:32:43,110 --> 00:32:47,570
+موجودة بكمية أقل تمام، بدي أعرف بزيادة ال
+
+462
+00:32:47,570 --> 00:32:51,010
+quantitative analysis بيستخدموا جهاز اسمه الـ
+
+463
+00:32:51,010 --> 00:32:57,770
+densitometerجهاز اسمه densitometer يستخدم في تقنية
+
+464
+00:32:57,770 --> 00:33:02,090
+ال chromatography for quantitative determination
+
+465
+00:33:02,090 --> 00:33:07,490
+يعني هو جهاز instrument to detect the color .. the
+
+466
+00:33:07,490 --> 00:33:12,330
+intensity of color هو جهاز يستخدم لمقياس شدة لون
+
+467
+00:33:12,330 --> 00:33:15,810
+لثبوت اللي ظهر عندي في تقنية ال chromatography
+
+468
+00:33:15,810 --> 00:33:21,750
+عشان يعطيني quantitative determination لالمادةإذا
+
+469
+00:33:21,750 --> 00:33:25,490
+بيستخدم التقنية الـ chromatography for qualitative
+
+470
+00:33:25,490 --> 00:33:33,150
+و quantitative للكمي و الجودةpaper chromatography
+
+471
+00:33:33,150 --> 00:33:36,450
+ال paper احنا اليوم هنستخدم paper chromatography
+
+472
+00:33:36,450 --> 00:33:39,430
+ال paper chromatography حكينا ان ال stationary
+
+473
+00:33:39,430 --> 00:33:43,230
+phase عبارة عن paper و ال solvent ال mobile phase
+
+474
+00:33:43,230 --> 00:33:47,410
+بيكون ليكوين ال paper chromatography بحط عليها
+
+475
+00:33:47,410 --> 00:33:50,670
+العينات بحمل عليها العينات ان انا بدي احط الخليط
+
+476
+00:33:50,670 --> 00:33:53,930
+من المواد على ال paper على ال stationary phase على
+
+477
+00:33:53,930 --> 00:33:57,430
+هيئة ال spot بحط نقطة من هذا الخليط على هذه الورقة
+
+478
+00:33:57,970 --> 00:34:01,410
+بعدين الورقة بغمرها في ال solvent بغمرها dipped
+
+479
+00:34:01,410 --> 00:34:06,130
+into a solvent بغمرها في ال mobile phase بيبدأ
+
+480
+00:34:06,130 --> 00:34:09,410
+يطلع يمشي ال mobile phase عبر ال stationary phase
+
+481
+00:34:09,410 --> 00:34:13,570
+بيمشي السائل للأعلى بناء على خاصية ال capillary
+
+482
+00:34:13,570 --> 00:34:19,050
+action الخاصية الشعرية و طول ما هو ماشي السائل
+
+483
+00:34:19,050 --> 00:34:22,770
+بتنفصل الخليط إلى مكوناته بناء على إيش حكينا
+
+484
+00:34:22,770 --> 00:34:26,950
+الفصل؟ بناء على ال solubility و ال attractionمدى
+
+485
+00:34:26,950 --> 00:34:31,050
+ذائبيتها في الـ Mobile Face ومدى تجاذبها مع الـ
+
+486
+00:34:31,050 --> 00:34:36,450
+Stationary Face إذا أنا ده تكرار الكلام كلهالـ
+
+487
+00:34:36,450 --> 00:34:39,790
+principle of paper chromatography الـ capillary
+
+488
+00:34:39,790 --> 00:34:42,770
+action هى شىه هى movement of liquid within the
+
+489
+00:34:42,770 --> 00:34:47,490
+space of a porous material هى حركة ال mobile phase
+
+490
+00:34:47,490 --> 00:34:50,710
+عبر ال stationary phase ال capillary action هى
+
+491
+00:34:50,710 --> 00:34:53,410
+عبارة عن movement of liquid اللى هو ال mobile
+
+492
+00:34:53,410 --> 00:34:56,570
+phase within a porous material اللى هى ال
+
+493
+00:34:56,570 --> 00:35:00,430
+stationary phase بس بتعبير آخر هى حركة ال mobile
+
+494
+00:35:00,430 --> 00:35:04,890
+phase عبر ال stationary phaseالـ Liquid اللي هو
+
+495
+00:35:04,890 --> 00:35:08,230
+الـ mobile phase بيتحرك عبر ال paper بسبب أن
+
+496
+00:35:08,230 --> 00:35:12,850
+الجاذبية ال attraction التجاذب أقوى من قوة
+
+497
+00:35:12,850 --> 00:35:18,860
+الجاذبيةبناء على أي مبدأ هيتم الفصل وبناء على إيش
+
+498
+00:35:18,860 --> 00:35:22,760
+حكينا بناء على like dissolve like وبناء على ال
+
+499
+00:35:22,760 --> 00:35:26,900
+solubility مع ال mobile phase و ال affinity أو ال
+
+500
+00:35:26,900 --> 00:35:30,940
+attraction with stationary phase إذا ال principle
+
+501
+00:35:30,940 --> 00:35:36,080
+لو بدي ألخصه هو حاجتين ال affinity with mobile ال
+
+502
+00:35:36,080 --> 00:35:39,740
+affinity أو attraction with stationary phase و
+
+503
+00:35:39,740 --> 00:35:45,480
+solubility with mobile phaseالـ Thin Layer
+
+504
+00:35:45,480 --> 00:35:51,280
+Chromatography هي تقنية لفصل المواد بيكون فيها الـ
+
+505
+00:35:51,280 --> 00:35:55,260
+stationary phase عبارة عن Thin Layer عبارة عن طبقة
+
+506
+00:35:55,260 --> 00:35:59,940
+خفيفة رفيعة of silica بتكون عبارة عن هذا الطبقة
+
+507
+00:35:59,940 --> 00:36:05,300
+سيليكا أو ألمنيوم سيليكا مدعمة بجزاز أو ألمنيوم
+
+508
+00:36:05,300 --> 00:36:09,950
+برضه لتدعيمإذا الـ thin layer chromatography هي
+
+509
+00:36:09,950 --> 00:36:13,350
+عبارة عن سيليكا بيكون ال stationary phase ورقة
+
+510
+00:36:13,350 --> 00:36:18,870
+رفية من السيليكا مدعمة بجزاز أو ألمنيوم السيليكا
+
+511
+00:36:18,870 --> 00:36:22,350
+جيل هتكون هي ال stationary phase أما ال solvent هو
+
+512
+00:36:22,350 --> 00:36:25,650
+ال mobile phase إذا ال thin layer chromatography
+
+513
+00:36:25,650 --> 00:36:29,770
+بدك تكون عارفة إن هي عبارة عن سيليكا ال stationary
+
+514
+00:36:29,770 --> 00:36:33,770
+phase عبارة عن سيليكا و ال solvent هو ال mobile
+
+515
+00:36:33,770 --> 00:36:40,250
+phaseإيش مبدأ الفصل ال principle ملخص ال principle
+
+516
+00:36:40,250 --> 00:36:44,670
+كله حكيناه إنه عبارة عن affinity with stationary
+
+517
+00:36:44,670 --> 00:36:50,570
+phase و attraction with mobile with stationary
+
+518
+00:36:50,570 --> 00:36:53,650
+phase و solubility with mobile phase لجيت هذا
+
+519
+00:36:53,650 --> 00:36:57,030
+الكلام اللي هجيت مكتوب في ال principle كله تكرار
+
+520
+00:36:57,030 --> 00:37:00,530
+للي قلناها different compound in sample mixture
+
+521
+00:37:00,530 --> 00:37:03,030
+travel different distance according to how
+
+522
+00:37:03,030 --> 00:37:06,470
+stronglythey interact with the stationary phase
+
+523
+00:37:06,470 --> 00:37:10,370
+and mobile phase بيحكيلي المواد بتمشي اللي موجودة
+
+524
+00:37:10,370 --> 00:37:14,570
+في الخلط بتنفصلها و بتمشي مسافات مختلفة كل واحدة
+
+525
+00:37:14,570 --> 00:37:18,750
+بتقف في مكان في مسافة مختلفة في مكان مختلف بناء
+
+526
+00:37:18,750 --> 00:37:21,910
+على ال attraction بناء على ال interact بناء على
+
+527
+00:37:21,910 --> 00:37:25,770
+التجادب مع ال stationary phase و ال mobile phase
+
+528
+00:37:31,430 --> 00:37:37,310
+المواد اللي موجودة في الخليط بتختلف في قوة ذائبية
+
+529
+00:37:37,310 --> 00:37:41,470
+والالتصاق مع ال stationary أو مع ال mobile phase
+
+530
+00:37:41,470 --> 00:37:44,850
+ال mobile phase اللي هو ال liquid أو ال eloquent
+
+531
+00:37:44,850 --> 00:37:46,110
+نفس مسمى
+
+532
+00:37:49,820 --> 00:37:53,080
+وهو يرتفع ببطء بيقول إن السائل اللي هو الـ mobile
+
+533
+00:37:53,080 --> 00:37:57,280
+phase بيمشي عبر الـ stationary phase اللي موجود في
+
+534
+00:37:57,280 --> 00:38:00,580
+الـ thin layer كروماتجرافي بالخاصية الشعرية بالـ
+
+535
+00:38:00,580 --> 00:38:04,680
+capillary action separation of compound is based
+
+536
+00:38:04,680 --> 00:38:07,940
+on the competition of the solute and the mobile
+
+537
+00:38:07,940 --> 00:38:11,740
+phase for binding substances بيقول لي إن فصل
+
+538
+00:38:11,740 --> 00:38:15,860
+المواد بناء على التنافس بين ال mobile phase و ال
+
+539
+00:38:15,860 --> 00:38:19,680
+stationary phase على هذه الموادمين ياخد مواد أكتر
+
+540
+00:38:19,680 --> 00:38:24,180
+وهذا بناء على solubility انا جيت اللي احنا
+
+541
+00:38:24,180 --> 00:38:27,840
+بنستخدمها هي thin layer chromatography و الـ thin
+
+542
+00:38:27,840 --> 00:38:33,040
+layer حكينا انه عبارة عن silica gel و السيليكا هي
+
+543
+00:38:33,040 --> 00:38:36,260
+polar فبيحكي لي if normal phase اذا احنا استخدمنا
+
+544
+00:38:36,260 --> 00:38:40,040
+silica gel as stationary phase ال stationary phase
+
+545
+00:38:40,040 --> 00:38:43,880
+يكون عندنا silica gel فهو polar فانا لو حطيت
+
+546
+00:38:43,880 --> 00:38:48,160
+مادتين بيختلفوا في ال polarity تمام؟المادة اللي
+
+547
+00:38:48,160 --> 00:38:51,620
+هتكون Polar، More Polar، Stronger Attraction
+
+548
+00:38:51,620 --> 00:38:55,740
+والسيلكا، مدام خصائصها Polar، المادة هي Polar، إذا
+
+549
+00:38:55,740 --> 00:38:59,080
+ان بتشبه خصائص السيلكا، هي Polar زيها، إذا انها
+
+550
+00:38:59,080 --> 00:39:02,800
+تلزج في الـstationary phase في السيلكا ومش هتمشي
+
+551
+00:39:02,800 --> 00:39:07,160
+للأعلى أما المواد الـNon Polar هي اللي هتمشي
+
+552
+00:39:07,160 --> 00:39:11,890
+لأعلى، move higherup to the plate فهتمشي أعلى
+
+553
+00:39:11,890 --> 00:39:16,850
+الأسرع للأعلى مع ال mobile phase لأن خصائصها تشبه
+
+554
+00:39:16,850 --> 00:39:20,270
+خصائص ال mobile هي non polar زي ال mobile لأن ال
+
+555
+00:39:20,270 --> 00:39:25,850
+stationary silica وهي polar فهذا
+
+556
+00:39:25,850 --> 00:39:29,530
+ال principle كله كلام زي اللي حكيناه ملخص ال
+
+557
+00:39:29,530 --> 00:39:34,490
+principle كله بكلمتين هي عبارة عن attraction with
+
+558
+00:39:34,490 --> 00:39:38,610
+stationary phase و solubility with mobile phase
+
+559
+00:39:40,250 --> 00:39:43,730
+retention factor حكينا عنه هو العامل المحافظة
+
+560
+00:39:43,730 --> 00:39:48,390
+بيحكي إنه كل مادة كيميائية لها retention factor
+
+561
+00:39:48,390 --> 00:39:54,830
+خاص فيها من خلالها بعرف هذه المادة كل مادة لها
+
+562
+00:39:54,830 --> 00:39:58,570
+retention factor خاص فيها أنا بحسب retention
+
+563
+00:39:58,570 --> 00:40:02,330
+factor بعد ما تفصل المواد بحسب لكل مادة retention
+
+564
+00:40:02,330 --> 00:40:06,520
+factorحكينا قانون ال retention factor إيش هو هو
+
+565
+00:40:06,520 --> 00:40:10,940
+المسافة اللي قطعتها المادة من البداية لون وصلت على
+
+566
+00:40:10,940 --> 00:40:14,280
+المسافة اللي قطعها ال solvent ال mobile phase من
+
+567
+00:40:14,280 --> 00:40:19,160
+البداية لون وصلت زي ما أنتوا شايفين هنا مثلا أجد
+
+568
+00:40:19,160 --> 00:40:21,980
+خط البداية اللي هو تحت أنا حاطيت الخليط من الخط
+
+569
+00:40:21,980 --> 00:40:26,200
+اللي تحت النقطة الحمراء مثلا بجيس المسافة بالمسطرة
+
+570
+00:40:26,200 --> 00:40:30,320
+من البداية لون هي وصلت على المسافة اللي مشيها
+
+571
+00:40:30,320 --> 00:40:35,520
+المحلو من خط البداية للنهايةوبشوفها بجسم على بعض
+
+572
+00:40:35,520 --> 00:40:39,000
+هذا الناتج وبيطلع عندي هذا ال retention factor
+
+573
+00:40:39,000 --> 00:40:47,020
+برجع للجدول المعروف و بشوف جداش لكل مادة the
+
+574
+00:40:47,020 --> 00:40:49,680
+stationary phase in thin layer chromatography نيجي
+
+575
+00:40:49,680 --> 00:40:54,080
+نحكي عن ال thin layer chromatographyالـ Thin Layer
+
+576
+00:40:54,080 --> 00:40:56,600
+Chromatography حكينا إن الـ Stachyna Rephase هي
+
+577
+00:40:56,600 --> 00:41:00,220
+عبارة عن Silica Gel و Silica Gel عبارة عن Polar
+
+578
+00:41:00,220 --> 00:41:03,860
+نيجي نتعرف على Silica Gel بيحكي إن Silica Gel is a
+
+579
+00:41:03,860 --> 00:41:06,000
+form of Silicon Dioxide هي عبارة عن Silicon
+
+580
+00:41:06,000 --> 00:41:10,900
+Dioxide بتكون من Silicon أتم من ذرات السيليكون
+
+581
+00:41:10,900 --> 00:41:15,560
+مرتبطة بجزيئات أكسجين ال structure الموجود فوق في
+
+582
+00:41:15,560 --> 00:41:19,220
+أقصى اليمين في الصورة من الأعلى هي عبارة عن ذرات
+
+583
+00:41:19,220 --> 00:41:25,100
+السيليكون الأسئعي مرتبطة مع الأكسجينوعلى السطح في
+
+584
+00:41:25,100 --> 00:41:29,540
+OH group attached to it إذا إن هي سيليكون ذرات من
+
+585
+00:41:29,540 --> 00:41:33,600
+السيليكون وموجود مرتبط معها أكسجين وعلى السطح في
+
+586
+00:41:33,600 --> 00:41:38,260
+OH الـ OH هي اللي خلتها بولا ال surface of silica
+
+587
+00:41:38,260 --> 00:41:43,340
+is بولا السطح عبارة عن OH فهو بولا can form
+
+588
+00:41:43,340 --> 00:41:48,530
+hydrogen bond ويقدر يعمل رابطة هيدروجينيةبما أنه
+
+589
+00:41:48,530 --> 00:41:52,790
+سطحها بولر إذا هو عبارة عن بولر وهي بيحكي اللي
+
+590
+00:41:52,790 --> 00:41:57,550
+بتكون مدعمة بألمنيوم أو جزاز وممكن تكون أصلا مش أي
+
+591
+00:41:57,550 --> 00:42:00,310
+نتس اللي كيكون نوع آخر لل stationary phase ممكن
+
+592
+00:42:00,310 --> 00:42:04,130
+يكون aluminium oxide يكون عبارة عن ألمنيوم مع
+
+593
+00:42:04,130 --> 00:42:09,520
+أكسوجين والسطح برضه قوات فهيكون بولر زيهابشكل عام
+
+594
+00:42:09,520 --> 00:42:11,720
+إن الـ stationary phase في الـ thin layer
+
+595
+00:42:11,720 --> 00:42:16,260
+كروماتجرافي عبارة عن سيليكون عبارة عن سيليكا جيل و
+
+596
+00:42:16,260 --> 00:42:21,000
+السيليكا على سطحها OH فالـ OH يعني polar فالـ thin
+
+597
+00:42:21,000 --> 00:42:25,520
+layer كروماتجرافي لو حكيتلك استخدام التقنية
+
+598
+00:42:25,520 --> 00:42:30,180
+الكروماتجرافي الـ thin layer كروماتجرافي بدك تفهمي
+
+599
+00:42:30,180 --> 00:42:34,060
+لحالك إن الـ stationary phase polar و ال mobile
+
+600
+00:42:34,060 --> 00:42:38,400
+phase non polarلو جبتلك هذا السؤال مثلا انه عندك
+
+601
+00:42:38,400 --> 00:42:41,380
+stationary phase عندك 10 layer chromatography و
+
+602
+00:42:41,380 --> 00:42:45,700
+عندك مادة A و مادة B A كانت polar و B non polar
+
+603
+00:42:45,700 --> 00:42:50,420
+مين اللي هتمشي إلى الفقر الأعلى أكتر؟ اذا انا بدي
+
+604
+00:42:50,420 --> 00:42:53,080
+افهم لحالي 10 layer chromatography إذا ال
+
+605
+00:42:53,080 --> 00:42:57,420
+stationary phase بدي يكون polar فالمادة A إذا كانت
+
+606
+00:42:57,420 --> 00:43:03,300
+هي ال polar هي اللي هتمشيإذا المادة A هي ال bowler
+
+607
+00:43:03,300 --> 00:43:07,640
+إذا ان هي مش هتمشي هي اللي هتضلها لتحت بس إذا
+
+608
+00:43:07,640 --> 00:43:12,600
+المادة B ال non bowler هي اللي هتمشي لفوق لأنه
+
+609
+00:43:12,600 --> 00:43:15,060
+thin layer chromatography بتدافع مع طول ال
+
+610
+00:43:15,060 --> 00:43:19,020
+stationary phase بيكون bowler فالمادة ال bowler
+
+611
+00:43:19,020 --> 00:43:22,020
+هتلزج و مش هتمشي للأعلى و المادة ال non bowler هي
+
+612
+00:43:22,020 --> 00:43:27,720
+اللي هتمشي للأعلىطب في عندنا تقنية fossil اسمها
+
+613
+00:43:27,720 --> 00:43:31,480
+Electrophoresis في عندنا احنا اليوم حكينا عن تقنية
+
+614
+00:43:31,480 --> 00:43:34,760
+ال chromatography في عندنا تقنية تانية اسمها
+
+615
+00:43:34,760 --> 00:43:40,720
+Electrophoresis لنقارن بين التقانيتين بحكيلي بناء
+
+616
+00:43:40,720 --> 00:43:43,360
+على ال definition بناء على التعريف ال
+
+617
+00:43:43,360 --> 00:43:47,540
+chromatography معناها fossil خليط من عدة مكونات
+
+618
+00:43:47,540 --> 00:43:50,260
+بناء على two phases اللي ال stationary phase و ال
+
+619
+00:43:50,260 --> 00:43:54,650
+mobile phaseإذن تقنية الـ chromatography هي فصل
+
+620
+00:43:54,650 --> 00:43:58,010
+خليط من المواد بناء على ال stationary phase و ال
+
+621
+00:43:58,010 --> 00:44:04,050
+mobile phase أما ال electrophoresis هي فصل خليط من
+
+622
+00:44:04,050 --> 00:44:10,550
+عدة مكونات بناء على الخصائص الكهربائية على ال
+
+623
+00:44:10,550 --> 00:44:14,190
+electrical field هو فصل بناء على الخصائص
+
+624
+00:44:14,190 --> 00:44:16,890
+الكهربائية ال electrophoresis أما ال
+
+625
+00:44:16,890 --> 00:44:20,730
+chromatography هو فصل بناء على stationary phase و
+
+626
+00:44:20,730 --> 00:44:26,720
+mobile phaseالعينة اللى ممكن استخدمها في حالة ال
+
+627
+00:44:26,720 --> 00:44:31,020
+chromatography ايش العينات اللى بقدر افصلها ممكن
+
+628
+00:44:31,020 --> 00:44:35,120
+افصل عينات تكون liquid ممكن تكون solid ممكن gas
+
+629
+00:44:35,120 --> 00:44:41,100
+بيقدر يفصل عينات تكون سائلة ممكن صلبة ممكن غازية
+
+630
+00:44:41,100 --> 00:44:47,700
+ال electrophoresis بيقدر يفصل عينات تكون سائلة و
+
+631
+00:44:47,700 --> 00:44:52,710
+صلبة فقط بيقدرش يفصل الغازالـ Principle المبدأ
+
+632
+00:44:52,710 --> 00:44:55,990
+للكرومتографية حكى هنا إن المبدأ الكرومتографي الـ
+
+633
+00:44:55,990 --> 00:45:00,170
+Principle هو attraction with stationary phase و
+
+634
+00:45:00,170 --> 00:45:04,150
+solubility with mobile phase أما الـ
+
+635
+00:45:04,150 --> 00:45:07,910
+Electrophoresis هي فصل بناء على الـ electrical
+
+636
+00:45:07,910 --> 00:45:12,550
+field بناء على الخصائص الكهربائية بناء على ال
+
+637
+00:45:12,550 --> 00:45:17,530
+electrical properties إذن هو فصل بناء على الخصائص
+
+638
+00:45:17,530 --> 00:45:21,460
+الكهربائيةالـ Principle لأ للـ Electrophoresis
+
+639
+00:45:21,460 --> 00:45:24,840
+بناء على الخصائص الكهربائية بناء على الـ
+
+640
+00:45:24,840 --> 00:45:28,200
+Chromatography بناء على ال attraction with mobile
+
+641
+00:45:28,200 --> 00:45:32,180
+phase with stationary phase with solubility with
+
+642
+00:45:32,180 --> 00:45:33,600
+mobile phase
+
diff --git a/PL9fwy3NUQKwZ1Q7zOg1ObgufPMjRI3WY1/MUWHefMMBZ4_postprocess.srt b/PL9fwy3NUQKwZ1Q7zOg1ObgufPMjRI3WY1/MUWHefMMBZ4_postprocess.srt
new file mode 100644
index 0000000000000000000000000000000000000000..8dbaf45588d427ab9ae6f7c9d7d24401d0fdc3fc
--- /dev/null
+++ b/PL9fwy3NUQKwZ1Q7zOg1ObgufPMjRI3WY1/MUWHefMMBZ4_postprocess.srt
@@ -0,0 +1,1812 @@
+1
+00:00:00,220 --> 00:00:03,140
+عملنا qualitative analytical test for
+
+2
+00:00:03,140 --> 00:00:08,500
+carbohydrates اللي هي ما عملنا بيحكي عن أنه فحوصات
+
+3
+00:00:08,500 --> 00:00:14,360
+نوعية فحوصات جودة للكشف عن ال carbohydrates عن
+
+4
+00:00:14,360 --> 00:00:18,800
+السكريات اليوم فحوصاتنا للكشف عن ال carbohydrate
+
+5
+00:00:18,800 --> 00:00:23,180
+وهي فحوصات qualitative فحوصات جودة يعني بيطلع
+
+6
+00:00:23,180 --> 00:00:26,940
+معايا في الآخر لون هيكون positive أو negative مش
+
+7
+00:00:26,940 --> 00:00:32,480
+هيطلع معايا كمية أو رقمماشي أهداف معملنا اليوم
+
+8
+00:00:32,480 --> 00:00:35,660
+بشكل عام المعمل كله بدنا نطلع منه general
+
+9
+00:00:35,660 --> 00:00:38,760
+information about carbohydrates بدنا نطلع منه
+
+10
+00:00:38,760 --> 00:00:41,740
+بمعلومات عامة عن الكربوهيدرات نعرف شو هي
+
+11
+00:00:41,740 --> 00:00:44,840
+الكربوهيدرات differentiate between carbohydrates
+
+12
+00:00:44,840 --> 00:00:49,420
+أني بدي أقارن .. بدي أفرد من أنواع الكربوهيدرات
+
+13
+00:00:49,420 --> 00:00:53,320
+أنواع السكريات qualitative analysis of
+
+14
+00:00:53,320 --> 00:00:57,000
+carbohydrates بعدين نشوف الفحوصات أو الجزء العملي
+
+15
+00:00:57,000 --> 00:01:02,240
+اللي هو فحوصات qualitativeللكشف عن السكريات
+
+16
+00:01:03,510 --> 00:01:06,930
+Equalitative Test of Amino Acids Equalitative
+
+17
+00:01:06,930 --> 00:01:10,790
+Analytical Test إيش هي qualitative؟ it is
+
+18
+00:01:10,790 --> 00:01:13,810
+concerned with determining the identity of a
+
+19
+00:01:13,810 --> 00:01:17,930
+substance لمعرفة هوية هذه المادة بس بدي أعرف هل
+
+20
+00:01:17,930 --> 00:01:21,050
+هذه المادة موجودة أو لا مثلا سكر موجود أو لا
+
+21
+00:01:21,050 --> 00:01:25,710
+enable us to detect the presence of things برضه
+
+22
+00:01:25,710 --> 00:01:30,230
+نفس الكلام إنه أعرف هل هذه الموجودة الأشياء أو لا
+
+23
+00:01:30,950 --> 00:01:37,030
+بحوصاتك qualitative يعني موجود أو غير موجود بداية
+
+24
+00:01:37,030 --> 00:01:40,330
+بدنا نتعرف على ال carbohydrates على السكرية
+
+25
+00:01:40,330 --> 00:01:45,070
+السكرية تم قسم إلى قسمين في منها simple saccharide
+
+26
+00:01:45,070 --> 00:01:47,930
+هي السكرية يعني simple سكرية وفي منها
+
+27
+00:01:47,930 --> 00:01:53,060
+polysaccharide سكرية بسيطة وسكرية تعدينةفي البداية
+
+28
+00:01:53,060 --> 00:01:56,020
+نيجي نحكي عن الـ simple saccharide الـ saccharide
+
+29
+00:01:56,020 --> 00:02:00,420
+هي من خصائصها إنه إلها low molecular weight الـ
+
+30
+00:02:00,420 --> 00:02:03,780
+molecular weight الوزن الجزائي إلها قليل وكمان هي
+
+31
+00:02:03,780 --> 00:02:08,600
+soluble in water بتتوق في الميه وكمان هي sweet to
+
+32
+00:02:08,600 --> 00:02:13,840
+طارس طعمها حلو إذا الـ saccharide الوزن الجزائي
+
+33
+00:02:13,840 --> 00:02:19,310
+إلها قليل ذائبة في الماء وطعمها حلوبتنقسم إلى ..
+
+34
+00:02:19,310 --> 00:02:23,170
+أنا مكتبت قسمياً هما تلاتة في منها mono-saccharide
+
+35
+00:02:23,170 --> 00:02:28,750
+سكريات أحادية في di-saccharide سكريات ثنائية في
+
+36
+00:02:28,750 --> 00:02:33,990
+oligosaccharide ألاقيت mono-saccharide سكر واحد،
+
+37
+00:02:33,990 --> 00:02:37,250
+دي بس واحد، سكر واحد، simple sugar di-saccharide
+
+38
+00:02:37,250 --> 00:02:40,970
+هي double sugar، بيكون سكر ثنائي اتنين من الـ mono
+
+39
+00:02:40,970 --> 00:02:45,070
+بيتحدوا مع بعض oligo بتكون من تلاتة إلى تسعة، عدد
+
+40
+00:02:45,070 --> 00:02:49,480
+السكريات فيها من تلاتة إلى تسعةالـ Monosaccharide
+
+41
+00:02:49,480 --> 00:02:54,140
+هي simple sugar هو سكر واحد بيكون الصيغة العامة له
+
+42
+00:02:54,140 --> 00:03:04,170
+CH2O لكل N N هي عدد ذرات الكعكصيغة له CH2O كل N N
+
+43
+00:03:04,170 --> 00:03:07,670
+هي عدد الذرات الكربون مثال عليها الـ
+
+44
+00:03:07,670 --> 00:03:12,330
+glyceraldehyde وهذا الـ glyceraldehyde فيه 3 ذرات
+
+45
+00:03:12,330 --> 00:03:18,390
+كربون فبتكون الصيغة العامة له C3H6O3
+
+46
+00:03:19,600 --> 00:03:24,400
+في عندنا جيليكوز، فراكتوز، جالاكتوز، هدولة mono
+
+47
+00:03:24,400 --> 00:03:29,120
+-saccharide، سكريات إحادية، hexose بنسميها هم
+
+48
+00:03:29,120 --> 00:03:32,860
+التلاتة الجيليكوز و فراكتوز و جالاكتوز، بنسميهم
+
+49
+00:03:32,860 --> 00:03:36,720
+hexose، سكرية simple-saccharide، mono-saccharide،
+
+50
+00:03:36,720 --> 00:03:41,460
+ولكن سوداسي، عدد ذرّات الكربون فيه 6في عندنا الـ
+
+51
+00:03:41,460 --> 00:03:46,080
+ribose و الـ manose و الـ xylose و الـ arabinose
+
+52
+00:03:46,080 --> 00:03:50,860
+هدولة فيهم خمس ذرات كربون بنطلق عليهم باسم Pentose
+
+53
+00:03:50,860 --> 00:03:55,340
+لذالن الـ monosaccharide هي simple sugar فيها سكر
+
+54
+00:03:55,340 --> 00:04:00,880
+واحد الصيغة العامة يلها CH2 all كل M أمثل عليها
+
+55
+00:04:00,880 --> 00:04:04,580
+اللي هو الـ glyceraldehyde بتكون من تلت ذرات كربون
+
+56
+00:04:05,010 --> 00:04:08,970
+ويعنى Gelicose وFructose وGalactose هدولة هكسوز
+
+57
+00:04:08,970 --> 00:04:13,630
+وكريات فيها 6 ذرات كاربوم نسميها هكسوز فيعنى
+
+58
+00:04:13,630 --> 00:04:20,330
+Ribose ومالنوز Ribose ومالنوز زيلوز أرابينوز هدولة
+
+59
+00:04:20,330 --> 00:04:26,280
+خماسي نسميها Pentoseأما الـ Disaccharide هو Double
+
+60
+00:04:26,280 --> 00:04:29,960
+Sugar سكة تنائية بتكون من اتنين Monosaccharide
+
+61
+00:04:29,960 --> 00:04:37,580
+بيتتحدوا مع بعض الصيغة العامة له C12H22O11
+
+62
+00:04:37,580 --> 00:04:42,980
+هي سكرين تنائيين بيجوا بيتتحدوا مع بعض بتكون من ست
+
+63
+00:04:42,980 --> 00:04:46,980
+ذرات كاربون يعني الصيغة أصلاً لكل واحد منهم هي
+
+64
+00:04:46,980 --> 00:04:49,700
+C12H
+
+65
+00:04:53,840 --> 00:04:57,420
+C6H12O6 إذن الـ «تايسا كرايد» هو اتحاد سكريين
+
+66
+00:04:57,420 --> 00:05:01,340
+تنائيين عبارة عن «هيكسوز» عبارة عن «سوداسي» C6 للـ
+
+67
+00:05:01,340 --> 00:05:04,920
+«رات كاربون» يعني الصيغة لكل واحد منهم من هدول
+
+68
+00:05:04,920 --> 00:05:11,200
+الوحيد اللي قاشوا اتحدوا مع بعض هي «C6H12O6»
+
+69
+00:05:11,880 --> 00:05:17,200
+يجي اتنين مع بعض C6 H12 O6 زائد C6 H12 O6 بيتحدوا
+
+70
+00:05:17,200 --> 00:05:22,520
+مع بعض افترض يعطيني Dice Caride سيغته العامة C12
+
+71
+00:05:22,520 --> 00:05:28,880
+H24 O12 طب هنا مكتوب ان الـ Dice Caride السيغة
+
+72
+00:05:28,880 --> 00:05:36,810
+العامة له C12 H22 O11موين راحت؟ ذرتين هيدروجين
+
+73
+00:05:36,810 --> 00:05:41,770
+وذرة أكسوجين مفقودين مفقودين في تكوين الرابط
+
+74
+00:05:41,770 --> 00:05:48,110
+السكرية بين السكريان والوحديان ارتباط سكريان
+
+75
+00:05:48,110 --> 00:05:53,130
+ووحديان مع بعض عشان يكونوا السكر التنائي بيرتبطوا
+
+76
+00:05:53,130 --> 00:05:59,080
+مع بعض برابط سكريةاسمها glycosidic bond هاي الـ
+
+77
+00:05:59,080 --> 00:06:05,840
+glycosidic point عندما تتكون ينتج عنها جزء ماء،
+
+78
+00:06:05,840 --> 00:06:11,380
+نزع جزء ماء فاسم التفاعل اللي بينتج عنه نزع جزء
+
+79
+00:06:11,380 --> 00:06:17,200
+ماء هو Condensation Reaction نفس التفاعل تكون
+
+80
+00:06:17,200 --> 00:06:21,470
+الرابط الـ PPD اللي حكينا عنهفعشان هي صيغة
+
+81
+00:06:21,470 --> 00:06:27,410
+السكريات السونية صارت C12H22O11
+
+82
+00:06:27,410 --> 00:06:32,610
+ونزعنا جزء ماء لتكوين الرابط السكرية اللي اسمها
+
+83
+00:06:32,610 --> 00:06:36,760
+glycosidic bondطيب، إيش الأمثلة على الـ
+
+84
+00:06:36,760 --> 00:06:41,400
+Disaccharide؟ عندنا Maltose، Saccharose و Lactose،
+
+85
+00:06:41,400 --> 00:06:45,680
+هدول سكريات و نايم حكينا إن هما عبارة عن اتحاد
+
+86
+00:06:45,680 --> 00:06:49,900
+سكريين أحدين مع بعض لو جينا حكينا عن الـ Maltose،
+
+87
+00:06:49,900 --> 00:06:53,680
+الـ Maltose هو عبارة عن اتحاد جليكوز مع جليكوز،
+
+88
+00:06:53,680 --> 00:06:57,900
+بيتحدوا مع بعض، بيكونولي Maltose الـ Saccharose هو
+
+89
+00:06:57,900 --> 00:07:03,060
+عبارة عن اتحاد جليكوز مع Efractoseالـ Lactose هو
+
+90
+00:07:03,060 --> 00:07:09,080
+عبارة عن اتحاد جليكوز مع Galactose لذا الملتوز
+
+91
+00:07:09,080 --> 00:07:14,440
+جليكوز و جليكوز السكاروز جليكوز و فراكتوز اللاكتوز
+
+92
+00:07:14,440 --> 00:07:19,300
+هو جليكوز و Galactose والـ Oligo حكينا عنها أنها
+
+93
+00:07:19,300 --> 00:07:24,080
+بتتكون من 3 إلى 9 سكريات مع بعض أما بالنسبة لـ
+
+94
+00:07:24,080 --> 00:07:27,920
+Polysaccharide اللي هي عديدة التسكر هي Complex
+
+95
+00:07:27,920 --> 00:07:34,330
+Sugar هي سكريات معقدةمن خصائصها إنه إلها high
+
+96
+00:07:34,330 --> 00:07:38,790
+molecular weight الوزن الجزائي لها عالي Insoluble
+
+97
+00:07:38,790 --> 00:07:42,350
+in water ما بتدوب في الماء لأنها عبارة عن سنسلة
+
+98
+00:07:42,350 --> 00:07:47,670
+طويلة بتفرعات فبيكون Insoluble كمان هي tasteless
+
+99
+00:07:47,670 --> 00:07:53,240
+لا طعم لها حكينا إنه هي multiple sugarتكون فيها
+
+100
+00:07:53,240 --> 00:07:57,740
+تفارع صيغة العامة ليها غير مطلوبة أمثلة عليها هي
+
+101
+00:07:57,740 --> 00:08:02,460
+الـ starch اللي هو النشا النباتي و الـ glycogen هو
+
+102
+00:08:02,460 --> 00:08:07,020
+النشا الحيواني الـ cellulose اللي بتستخدم النبات
+
+103
+00:08:07,020 --> 00:08:11,660
+لبناء الـcell wallاللينن والكايتن اللي بتستخدم
+
+104
+00:08:11,660 --> 00:08:15,620
+الحشرات لبناء ال cell wall ده من الأمثل على ال
+
+105
+00:08:15,620 --> 00:08:20,320
+polysaccharide star جناش النباتي وجلايكوجن نشا
+
+106
+00:08:20,320 --> 00:08:24,340
+حيواني cellulose اللي بيستخدم في النبات لبناء ال
+
+107
+00:08:24,340 --> 00:08:29,640
+cell wall اللينن والكايتن اللي بيستخدم في الحشرات
+
+108
+00:08:29,640 --> 00:08:34,900
+لبناء ال cell wallبينهاج اسمها الـ functional
+
+109
+00:08:34,900 --> 00:08:39,520
+group functional group هي منطقة في المُركّب تحدد
+
+110
+00:08:39,520 --> 00:08:43,720
+خصائصه هي region of molecules that give it a
+
+111
+00:08:43,720 --> 00:08:48,860
+particular property single molecule can have more
+
+112
+00:08:48,860 --> 00:08:51,620
+than one functional group as parts of its
+
+113
+00:08:51,620 --> 00:08:55,380
+structure ممكن يكون عنده أكتر من functional group
+
+114
+00:08:56,660 --> 00:08:59,560
+الـ active part أو الـ functional group اللي
+
+115
+00:08:59,560 --> 00:09:04,620
+موجودة في السكريات هي الديهيد أو كيتون group بتكون
+
+116
+00:09:04,620 --> 00:09:09,540
+الديهيد أو كيتون الديهيد أو كيتون and هيدروكسيل
+
+117
+00:09:09,540 --> 00:09:13,980
+group لذلك اللي موجود بيكون الديهيد أو كيتون أو
+
+118
+00:09:13,980 --> 00:09:16,700
+هيدروكسيل group هذا اللي بيكونوا موجودين في
+
+119
+00:09:16,700 --> 00:09:21,800
+السكريات هي الـ functional groupطلّع تحت إيش الفرق
+
+120
+00:09:21,800 --> 00:09:25,440
+بين الـ Aldehyde وإيش الفرق بينه وبين الـ Ketone
+
+121
+00:09:25,440 --> 00:09:31,620
+الـ Aldehyde بتكون عندنا C مرتبطة مع الـ double
+
+122
+00:09:31,620 --> 00:09:37,540
+bond A من جهة مرتبطة بذرّة هيدروجين ومن الجهة
+
+123
+00:09:37,540 --> 00:09:42,510
+التانية مرتبطة بـ R group، بمجموعةإذاً في الـ
+
+124
+00:09:42,510 --> 00:09:47,230
+aldehyde بتكون عنا الـ C double bond O مرتبطة من
+
+125
+00:09:47,230 --> 00:09:53,150
+جهة بذرة هيدروجين ومن جهة تانية مرتبطة بـ R group
+
+126
+00:09:53,150 --> 00:09:57,470
+مجموعة مش بذرة هيدروجين بسيطة فقط وإنما بمجموعة
+
+127
+00:09:57,470 --> 00:10:00,970
+إذا لاجينا الـ C double bond O من جهة مرتبطة بذرة
+
+128
+00:10:00,970 --> 00:10:04,950
+هيدروجين ومن جهة تانية بمجموعة بـ R group بسميها
+
+129
+00:10:04,950 --> 00:10:10,330
+aldehyde أما إذا شوفت الـ C double bond Oمرتبطة من
+
+130
+00:10:10,330 --> 00:10:14,690
+جهة بـ R-group مجموعة ومن جهة التانية كمان R-group
+
+131
+00:10:14,690 --> 00:10:19,930
+مجموعة بسميها كيتون لذن الـ aldehyde بتكون الـCWO
+
+132
+00:10:19,930 --> 00:10:24,970
+مرتبطة من جهة بـH ومن جهة بـR أما الكيتون بتكون
+
+133
+00:10:24,970 --> 00:10:31,990
+الـCWO مرتبطة من جهة بـR ومن جهة بـR تاني زي تحت
+
+134
+00:10:31,990 --> 00:10:35,410
+مثل عن الـGlucose و الـFructoseلو جينا تلالنا على
+
+135
+00:10:35,410 --> 00:10:38,190
+الـ Structure للـ Glycose نيجي نطلع الـ C double
+
+136
+00:10:38,190 --> 00:10:42,290
+bond O وينها، هي على الشمال، C double bond O محوطة
+
+137
+00:10:42,290 --> 00:10:47,450
+عليها، إيش مرتبطة من جهة مرتبطة بضارة هيدروجين؟ من
+
+138
+00:10:47,450 --> 00:10:51,610
+الجهة التانية مرتبطة بمجموعة، ب R group، اللي هي C
+
+139
+00:10:51,610 --> 00:10:57,640
+-O-H-Hإذا من جهة مرتبطة بـ H ومن جهة بـ R مين يبقى
+
+140
+00:10:57,640 --> 00:11:01,500
+هيكون Aldehyde ولا كيتون الجليكوز؟ أكيد هيكون
+
+141
+00:11:01,500 --> 00:11:05,360
+Aldehyde لأنه مرتبطة C double bond O من جهة بـ R
+
+142
+00:11:05,360 --> 00:11:10,060
+ومن جهة بـ H لو جينا للفركتوز مكتوب بالناس بال
+
+143
+00:11:10,060 --> 00:11:13,260
+structure C double bond O اللي هي محوط عليها من
+
+144
+00:11:13,260 --> 00:11:19,130
+جهة مرتبطةبـ R group اللي هي CHHOH من الجهة
+
+145
+00:11:19,130 --> 00:11:23,650
+التانية مرتبطة بـ CHOH برضه R تانية إذا الـ C
+
+146
+00:11:23,650 --> 00:11:27,630
+double bond O في الفركتوز متصلة من الجهتين بـ R من
+
+147
+00:11:27,630 --> 00:11:33,250
+جهة و R من جهة يبقى هتكون كيتونتمام؟ إذا الجليكوز
+
+148
+00:11:33,250 --> 00:11:37,850
+عبارة عن الديهايد والفركتوز عبارة عن كيتون أصبحنا
+
+149
+00:11:37,850 --> 00:11:42,230
+عارفين إيش الفرق بين الالديهايد وكيتون بحكينا إن
+
+150
+00:11:42,230 --> 00:11:47,970
+الالديهايد بتكون على كربونا رقم 1 والكيتون بيكون
+
+151
+00:11:47,970 --> 00:11:52,170
+على كربونا رقم 2 إنه بدي أرقم تقول لو جينا على
+
+152
+00:11:52,170 --> 00:11:55,330
+الجليكوز نيجي الرقم من الكربون نبدأ الترقيم من
+
+153
+00:11:55,330 --> 00:12:00,850
+الجهة الأقرب للـCW1الترقيم يبدأ من الجهة الأقرب
+
+154
+00:12:00,850 --> 00:12:04,730
+للـ CW من ضهر هذه الجهة من عند الـ functional
+
+155
+00:12:04,730 --> 00:12:07,390
+group من الجهة الأقرب للـ functional group من عند
+
+156
+00:12:07,390 --> 00:12:10,770
+الـ CW من ضهر هتطلع كربونا رقم واحد ومتصلة من جهة
+
+157
+00:12:10,770 --> 00:12:15,110
+الـ H ومن جهة المجموعة فالالدهيد في الجلوكوز الـ
+
+158
+00:12:15,110 --> 00:12:19,540
+CW من ضهر موجود على رقم كربونا رقم واحدلو جينا على
+
+159
+00:12:19,540 --> 00:12:22,760
+الـ Fructose جينا الرقم من اليسارها، الرقم هي في
+
+160
+00:12:22,760 --> 00:12:26,580
+عندي كربونا رقم 1، وبعدين 2 كربونا اللي هي C
+
+161
+00:12:26,580 --> 00:12:31,440
+double bond 1 في الـ Ketone، في الـ Fructose اللي
+
+162
+00:12:31,440 --> 00:12:37,860
+هي موجودة على كربونا رقم 2هنا حاجة اسمها Sugar
+
+163
+00:12:37,860 --> 00:12:43,560
+Nomenclature اللي هي السكر وصورته كأنه انعكسه في
+
+164
+00:12:43,560 --> 00:12:47,700
+المرايا كأنه mirror image أن السكر له انعكاس نفس
+
+165
+00:12:47,700 --> 00:12:53,820
+السكر موجود على شكلين في منه الـD وأقل بنسميهم
+
+166
+00:12:53,820 --> 00:12:56,760
+يعني مثلا الـGelicose في منه الـD-Gelicose وأقل
+
+167
+00:12:56,760 --> 00:13:00,940
+الـGelicose كيف هذا بدي أقرر هل هو الـD ولا الأقل؟
+
+168
+00:13:01,480 --> 00:13:05,080
+لما نكتب السكر الـ structure له بشكل linear بشكل
+
+169
+00:13:05,080 --> 00:13:10,540
+خطي بشكل عمودي بدي أرقم الـ carbon بيطلع على
+
+170
+00:13:10,540 --> 00:13:18,020
+carbonة رقم 5 بيطلع على carbonة رقم 5 إذا الـ OH
+
+171
+00:13:18,020 --> 00:13:23,560
+موجودة إلى جهة الـ left إلى جهة اليسار بيكون الـ 4
+
+172
+00:13:24,460 --> 00:13:29,280
+دي هتطلع على كربونا رقم 5 فنطلع على الـ Hydroxyl
+
+173
+00:13:29,280 --> 00:13:32,160
+group اللي متصلة فيها اللي هي الـ OH اللي متصلة
+
+174
+00:13:32,160 --> 00:13:37,020
+فيها إذا كانت على جهة الـ Left بيكون Alpha إذا
+
+175
+00:13:37,020 --> 00:13:41,900
+كانت الـ Hydroxyl group على كربونا 5 إلى جهة الـ
+
+176
+00:13:41,900 --> 00:13:47,390
+Right إلى جهة اليمينبكون «DF»، إذاً بدي أحدد هل هو
+
+177
+00:13:47,390 --> 00:13:53,210
+«D» ولا «L» برقم الكربونة، ببطل على كربونة رقم 5،
+
+178
+00:13:53,210 --> 00:13:56,150
+إذا الـ «OH» الـ hydroxyl group المتصلة فيها كانت
+
+179
+00:13:56,150 --> 00:14:00,010
+إلى جهة الـ «Left»، بتكون «Alpha»، إذا كانت إلى
+
+180
+00:14:00,010 --> 00:14:05,560
+جهة ال «Right»، بيكون «DF»بحكي لي most naturally
+
+181
+00:14:05,560 --> 00:14:09,720
+occurring sugars are D isomers إن معظم السكريات في
+
+182
+00:14:09,720 --> 00:14:14,020
+الجسم هي على شكل الـD form لكن الأحمض الأمنية في
+
+183
+00:14:14,020 --> 00:14:18,300
+الجسم موجودة على شكل الـForm لو جينا مثلا على هاي
+
+184
+00:14:18,300 --> 00:14:20,640
+الرسم، على الـStructure اللي على جانبها الجليكوز
+
+185
+00:14:20,640 --> 00:14:24,020
+نيجي نرقم، حكينا من وين نبدأ ترقيم، من عند
+
+186
+00:14:24,020 --> 00:14:26,880
+الـfunctional group يعني الـD الجليكوز، هذا اللي
+
+187
+00:14:26,880 --> 00:14:30,160
+مكتوب عليه D جليكوز كيف حكينا إنه D؟ كيف حددوا إنه
+
+188
+00:14:30,160 --> 00:14:33,740
+D؟بدي أجي أرقم من وين أبدأ الترقيم من تحت و لا من
+
+189
+00:14:33,740 --> 00:14:36,760
+فوق من عند الـ functional group اللي هي الـ C
+
+190
+00:14:36,760 --> 00:14:40,960
+double bond هو وين موجودة أقرب، فوق و لا تحت، فوق،
+
+191
+00:14:40,960 --> 00:14:45,440
+لذا هابدأ الترقيم من فوق عند Carbon رقم 1، 2، 3،
+
+192
+00:14:45,440 --> 00:14:50,200
+4، 5 عند Carbon رقم 5 اللي هي اللي جنبها OH معلمة
+
+193
+00:14:50,200 --> 00:14:53,660
+بالأصفر عند هذه الـ Carbon هبدأ أطلع على الـ OH
+
+194
+00:14:53,660 --> 00:14:56,520
+اللي هي الـ hydroxyl group هل موجودة إلى الـ left
+
+195
+00:14:56,520 --> 00:14:59,990
+ولا إلى ال right؟ إلى اليمين ولا للشمال؟موجودة على
+
+196
+00:14:59,990 --> 00:15:04,750
+اليمين إذا هو D form إذا هو D يجي لكاذب نيجي
+
+197
+00:15:04,750 --> 00:15:08,510
+للتاني بدي أرقم برضه من الجهة الأقرب لل functional
+
+198
+00:15:08,510 --> 00:15:12,290
+group اللي هي ال four برقم ال carbon واحد، اتنين،
+
+199
+00:15:12,290 --> 00:15:15,870
+تلاتة، أربعة، خمسة ال carbon الرقم خمسة اللي هي
+
+200
+00:15:15,870 --> 00:15:19,430
+معلمة بالأصفر وهي عندنا ال OH هل هي إلى ال left
+
+201
+00:15:19,430 --> 00:15:25,570
+ولا إلى ال right؟ لل left إذا هندقها left إذا هو
+
+202
+00:15:25,570 --> 00:15:31,380
+ال fourفي عندنا السُكر لما بدي أرسم الـ structure
+
+203
+00:15:31,380 --> 00:15:35,580
+للسُكريات في منها شكل إيه؟ في منها شكل Linear خطي
+
+204
+00:15:35,580 --> 00:15:41,040
+عمودي بيكون وفي منها شكل حلقة هلجيت الشكل الـ
+
+205
+00:15:41,040 --> 00:15:44,180
+Linear اللي عندى؟ حكينا بنقدر نكتب عليه هو الـ D
+
+206
+00:15:44,180 --> 00:15:47,720
+أو R هلجيت لو عند الـ Linear هذا و بتعمل منه حلقة
+
+207
+00:15:47,720 --> 00:15:54,590
+كيف؟ بربط Carbon رقم 5 مع الـ functional groupكيف
+
+208
+00:15:54,590 --> 00:16:00,070
+بدأ أحول من Linear أو عمودي لمغلق برابط Carbon رقم
+
+209
+00:16:00,070 --> 00:16:03,330
+5 مع الـ Functional Group ألقيت عندي حلقتين
+
+210
+00:16:03,330 --> 00:16:07,070
+هايومها، احكيلي الحلقة لما نسميها، بنسميها Alpha
+
+211
+00:16:07,070 --> 00:16:10,490
+أو Beta، هل هي Alpha أو Beta كيف بدها تكون Alpha
+
+212
+00:16:10,490 --> 00:16:16,490
+أو Beta، كيف بدأ أميز هل هي Alpha أو Beta؟بحكي ليه
+
+213
+00:16:16,490 --> 00:16:21,970
+بنطلع على Carbona رقم 1؟ أشوف الـ OH المتصل فيها،
+
+214
+00:16:21,970 --> 00:16:26,130
+هل هي إلى الأسفل أو للأعلى؟ إذا الـ OH المتصلة مع
+
+215
+00:16:26,130 --> 00:16:30,870
+Carbona رقم 1 كانت below، كانت للأسفل، أسفل
+
+216
+00:16:30,870 --> 00:16:36,060
+الحلقة، بنسميها Alphaإذا كانت الـ OH متصلة في
+
+217
+00:16:36,060 --> 00:16:40,880
+الكربونة رقم 1 للأعلى، بسميه Beta يعني لو جينا
+
+218
+00:16:40,880 --> 00:16:45,640
+الحلقتين الموجودين عندنا، بنرقم الكربون عند كل
+
+219
+00:16:45,640 --> 00:16:48,580
+نقطة، عند كل زاوية، بيكون فيها ذرّة كربون في
+
+220
+00:16:48,580 --> 00:16:52,120
+الحلقة هلجت هذه الكربون، بدي أرقمها، من أين نبدأ
+
+221
+00:16:52,120 --> 00:16:55,340
+ترقيم حكينا؟ من عند الـ functional groupكيف تتعرف
+
+222
+00:16:55,340 --> 00:16:57,700
+فى الـ functional group فى الحلقة؟ بتكون مرتبطة فى
+
+223
+00:16:57,700 --> 00:17:00,500
+الـA C double bond A مش هي بتكون مرتبطة فى الـA
+
+224
+00:17:00,500 --> 00:17:04,540
+فهى الـA هى الكربونة المتصلة فىها هي كربونة رقم 1
+
+225
+00:17:04,540 --> 00:17:10,860
+برقم 1,2,3,4,5,6 مركزين معايا على الحلقة الأولى
+
+226
+00:17:10,860 --> 00:17:15,430
+اللى علي الشمال أول حلقة هي مرقمةمن عند الـ
+
+227
+00:17:15,430 --> 00:17:20,770
+Function Algorithm 1,2,3,4,5,6 كربونة رقم 1 اللي
+
+228
+00:17:20,770 --> 00:17:24,690
+قد تطلع عليها When الـ OH هي بالأزرق الـ OH هل هي
+
+229
+00:17:24,690 --> 00:17:28,810
+للأسفل ولا للأعلى؟ للأسفل، إذن هو Alpha-Glucose
+
+230
+00:17:29,520 --> 00:17:33,320
+جينا على الحلقة التانية، بدي أرقم الـ Carbon، من
+
+231
+00:17:33,320 --> 00:17:36,500
+وين أرقم؟ من عند الـ Functional Group، واحد،
+
+232
+00:17:36,500 --> 00:17:41,000
+اتنين، تلاتة، اربعة، خمسة، ستة، هجد أشوف الـ OH
+
+233
+00:17:41,000 --> 00:17:44,540
+المتصلة في Carbon رقم واحد، هيو معلم ليها بالازرق
+
+234
+00:17:45,010 --> 00:17:49,150
+وين موجودة للأعلى أو للأسفل؟ موجودة الـ OH للأعلى
+
+235
+00:17:49,150 --> 00:17:53,550
+above the ring إذا هو Beta Glucose إذا لما بدي
+
+236
+00:17:53,550 --> 00:17:57,590
+أسمي الحلقة Alpha ولا Beta بناءً على الـ OH
+
+237
+00:17:57,590 --> 00:18:03,430
+المتصلة بكربونا رقم 1 هل هي للأعلى ولا للأسفل؟
+
+238
+00:18:04,720 --> 00:18:08,600
+بنحكي عن الـ mono-saccharide الـ mono-saccharide
+
+239
+00:18:08,600 --> 00:18:12,700
+حكينا إن هي سكريات وحادية و بتختلف في عدد الراتد
+
+240
+00:18:12,700 --> 00:18:16,760
+الكربون في منها هكسوسوداسية و في منها خماسية بنتوز
+
+241
+00:18:16,760 --> 00:18:20,220
+بيحكيلي في منها كمان السكريات إحنا حكينا بشكل عام
+
+242
+00:18:20,220 --> 00:18:23,740
+في منها عندها تحتوي على aldehyde group في منها
+
+243
+00:18:23,740 --> 00:18:27,540
+تحتوي على كيتونيجون فالـ mono-saccharide بيحكيلي
+
+244
+00:18:27,540 --> 00:18:32,260
+بتنقسم إلى قسمين في منها aldose و في منها ketose
+
+245
+00:18:33,210 --> 00:18:37,510
+ماشي يعني الـ Dose؟ يعني تحتوي على Aldehyde group
+
+246
+00:18:37,510 --> 00:18:41,770
+الـ Dose معناها إنه عندها Aldehyde group طب و
+
+247
+00:18:41,770 --> 00:18:45,030
+حكينا وين الـ Aldehyde group بتكون؟ حكينا بتكون
+
+248
+00:18:45,030 --> 00:18:50,870
+موجودة على كربونا رقم 1 أما Ketose معناها إنه
+
+249
+00:18:50,870 --> 00:18:55,970
+تحتوي على كيتون group و حكينا إن الكيتون بتكون على
+
+250
+00:18:55,970 --> 00:19:00,850
+كربونا رقم 2الذن الـ Dose اللى بتحتوي على الديهيد
+
+251
+00:19:00,850 --> 00:19:05,330
+جروب بتكون على كربونا رقم واحد أشهر مثال على ال ال
+
+252
+00:19:05,330 --> 00:19:09,670
+Dose هو الجلوكوز سكر الجلوكوز يحتوي على الديهيد
+
+253
+00:19:09,670 --> 00:19:15,470
+جروب فبسميه ال Dose و أصلا ال جلوكوز هو سكر هو
+
+254
+00:19:15,470 --> 00:19:20,630
+هكسوز يحتوي على ست ذرات كربو حكينا عنه هكسوز فبقدر
+
+255
+00:19:20,630 --> 00:19:26,470
+أطلق عليه اسم ال Dohexoseالجيلوكوز بقدر أطلق عليه
+
+256
+00:19:26,470 --> 00:19:30,530
+اسم Aldohexose من هون أجت كلمة Aldo لأنه عنده
+
+257
+00:19:30,530 --> 00:19:36,250
+Aldehyde group وhexose لأنه ست ذرات كرم فبالكيتوز
+
+258
+00:19:36,250 --> 00:19:40,270
+اللي تحتوي على كيتون group أشهر مثال عليها هو
+
+259
+00:19:40,270 --> 00:19:46,600
+الفركتوزوالـ Fructose أصلاً هو هكسوز هو يحتوي على
+
+260
+00:19:46,600 --> 00:19:52,300
+6 ذرات كربون اللي هو سداسي فبقدر أطلق عليه اسم
+
+261
+00:19:52,300 --> 00:19:58,280
+كيتوهكسوز كيتوهكسوز معناه أنه يحتوي على كيتون جروب
+
+262
+00:19:58,280 --> 00:20:05,110
+وهو سداسي يحتوي على 6 ذرات كربونبنحكي عن الـ
+
+263
+00:20:05,110 --> 00:20:08,190
+polysaccharide الـ polysaccharide حكينا هي العديد
+
+264
+00:20:08,190 --> 00:20:12,130
+تتسسكر من الأمثلة عليها، عندنا أول مثال الـ
+
+265
+00:20:12,130 --> 00:20:16,190
+amylose الـ amylose هو عبارة عن سلسلة من سكر
+
+266
+00:20:16,190 --> 00:20:20,430
+الجليكوز جليكوز بوليمر، هي الرسمة عندنا الـ
+
+267
+00:20:20,430 --> 00:20:24,690
+structure له للـ amylose أنه عبارة عن سكر الجليكوز
+
+268
+00:20:24,690 --> 00:20:29,620
+متصل مع بعض و لبعض، بوليمر من الجليكوزالفا واحد
+
+269
+00:20:29,620 --> 00:20:34,200
+لاربع لينكش ان هي الرابط الموجودة بينهم اسمها الفا
+
+270
+00:20:34,200 --> 00:20:38,500
+واحد لاربع ليش كيف سميناها الفا واحد لاربع؟ لجيت
+
+271
+00:20:38,500 --> 00:20:41,420
+الفا من وين أجت؟ حكينا من الطلع على كربونا رقم
+
+272
+00:20:41,420 --> 00:20:45,040
+واحد لو جينا على الحلقة الأولى على اليسار تيجي على
+
+273
+00:20:45,040 --> 00:20:49,380
+الكربونا لو طلعت على ال OH هل هي below ولا above؟
+
+274
+00:20:49,380 --> 00:20:55,470
+لاجي ال OH belowالواتش لتحت اسمها Alpha طيب واحد
+
+275
+00:20:55,470 --> 00:20:59,750
+لاربعة هذا اسم الموقع الرابطة بين الجلوكوز الأول
+
+276
+00:20:59,750 --> 00:21:03,370
+والجلوكوز التاني لو جينا على الجلوكوز الأول هيوم
+
+277
+00:21:03,370 --> 00:21:07,970
+رقم اللي على الشمال عندك واحد كربونا رقم واحد تصلت
+
+278
+00:21:07,970 --> 00:21:12,540
+مع كربونا رقم أربعة من الجلوكوز التانيباسم الرابط
+
+279
+00:21:12,540 --> 00:21:18,560
+ألفة واحد لاربعة ألفة عشان الـ OH لتحت واحد لاربعة
+
+280
+00:21:18,560 --> 00:21:22,840
+لأن كربونا رقم واحد من جلوكوزي الأول اتصلت مع
+
+281
+00:21:22,840 --> 00:21:28,090
+كربونا رقم أربعة من جلوكوزي التانيمثال تاني على
+
+282
+00:21:28,090 --> 00:21:31,530
+الـ polysaccharide اللي هو الـ amylobectin الـ
+
+283
+00:21:31,530 --> 00:21:35,850
+amylobectin بيشبه الـ amylose هو عبارة عن مجموعة
+
+284
+00:21:35,850 --> 00:21:39,670
+من الجلوكوز متصل مع بعض و لبعض فهو جلوكوز بوليمر
+
+285
+00:21:39,670 --> 00:21:43,810
+برابط ألف و واحد لأربعة و عرفنا ألفة جاية الـ OH
+
+286
+00:21:43,810 --> 00:21:47,410
+لتحت واحد لأربعة اللي هي كربونا رقم واحد السكر
+
+287
+00:21:47,410 --> 00:21:50,830
+الأول مع كربونا رقم أربعة من السكر التاني ارتبطوا
+
+288
+00:21:50,830 --> 00:21:54,690
+مع بعضبس الزيادة على الـ Amylose إنه عنده branch
+
+289
+00:21:54,690 --> 00:22:00,830
+عنده تفرع من سكر الجلوكوز مكون رابطة اسمها Alpha 1
+
+290
+00:22:00,830 --> 00:22:06,090
+لـ 6 Alpha 1 ل 6 Alpha من وين جاي؟ إنه الـ OH لتحت
+
+291
+00:22:06,090 --> 00:22:12,130
+1 ل 6 إنه التفرع جاي وين؟ عند Carbona رقم 1 من
+
+292
+00:22:12,130 --> 00:22:16,250
+الحلقة اللي فوق مع Carbona رقم 6 من الحلقة اللي
+
+293
+00:22:16,250 --> 00:22:21,180
+تحتإذًا الـ Amylose هو polysaccharide عبارة عن
+
+294
+00:22:21,180 --> 00:22:25,680
+جليكوز بوليمار الرابطة ألف واحد لأربعة Amylopectin
+
+295
+00:22:25,680 --> 00:22:29,920
+برضه هو polysaccharide جليكوز بوليمار عنده ألف
+
+296
+00:22:29,920 --> 00:22:36,700
+لواحد لأربعة وعنده كمان branch رابطة ألف واحد لستة
+
+297
+00:22:40,600 --> 00:22:43,920
+مثال آخر على الـ polysaccharide اللي هي عديد
+
+298
+00:22:43,920 --> 00:22:47,360
+التسكر هو الـ Glycogen الـ Glycogen زي الـ
+
+299
+00:22:47,360 --> 00:22:51,040
+amylobectin عنده رابط عنده polymer من جلكوز ألف
+
+300
+00:22:51,040 --> 00:22:54,880
+واحد لاربعة و عنده branch ألف واحد لستة لكن
+
+301
+00:22:54,880 --> 00:22:58,980
+التفرعات اللي عنده أكتر من تفرعات الـ amylobectin
+
+302
+00:22:58,980 --> 00:23:03,280
+زي الـ I-Glycogen more ألف واحد لستة branch
+
+303
+00:23:03,280 --> 00:23:08,800
+التفرعات زيانةوكل ما زادت التفرعات في السكر، هذا
+
+304
+00:23:08,800 --> 00:23:14,000
+بيجعل السكر ضعيف ويكون سهل تكسّم، فالـ Glycogen
+
+305
+00:23:14,000 --> 00:23:18,160
+عشان تفرعاته أكتر، فهذا بيخليه Rapid glucose
+
+306
+00:23:18,160 --> 00:23:22,620
+release from Glycogen، لما الجسم يحتاج glucose،
+
+307
+00:23:22,620 --> 00:23:26,660
+أسرع يجي أنه يكسّر الـ Glycogen ويعطيني glucose،
+
+308
+00:23:26,660 --> 00:23:31,520
+لأنه عنده تفرعات زيادة، فهذا بيتكسر بسرعة وبيحرر
+
+309
+00:23:31,520 --> 00:23:36,260
+سكر الـ glucose لما يحتاجه الجسم،ليش سهل التكسر
+
+310
+00:23:36,260 --> 00:23:41,980
+بسبب زيادة التفرعات مثال آخر هو الـ cellulose
+
+311
+00:23:41,980 --> 00:23:45,520
+حكينا الـ cellulose يستخدم في الـ plant cell wall
+
+312
+00:23:45,520 --> 00:23:49,800
+في بناء الـ cell wall للـ plant للنبات هو عبارة عن
+
+313
+00:23:49,800 --> 00:23:55,000
+linear chain سلسلة طويلة من السكر ولكن هي الرابطة
+
+314
+00:23:55,000 --> 00:24:01,030
+Beta 1L4بيتا ليش؟ لأن الـ OH للحلقة موجودة above
+
+315
+00:24:01,030 --> 00:24:07,550
+بوجودة للأعلى OH للأعلى، إذا بيكون بيتا 1 ل 4 لأن
+
+316
+00:24:07,550 --> 00:24:11,410
+الرابط بين كل حلقة والتانية جاية من كربون رقم واحد
+
+317
+00:24:11,410 --> 00:24:15,510
+من الحلقة الأولى مع كربون رقم أربع من الحلقة
+
+318
+00:24:15,510 --> 00:24:20,170
+التانية إذا الـ cellulose بيكون نينيار من الـ
+
+319
+00:24:20,170 --> 00:24:27,700
+glucose بوليمر من الـ glucose برابطة بيتا 1 ل 4فيه
+
+320
+00:24:27,700 --> 00:24:31,280
+إنه تقسيم للسكريات بناء على الـ reducing هل هو
+
+321
+00:24:31,280 --> 00:24:35,240
+reducing sugar أو non-reducing sugar في البداية
+
+322
+00:24:35,240 --> 00:24:37,500
+نحكي إيش يعني oxidation وإيش يعني reduction
+
+323
+00:24:37,500 --> 00:24:42,080
+oxidation معناها loss of electron إنه فقد إلكترون
+
+324
+00:24:42,080 --> 00:24:46,080
+بسميه oxidation reduction معناها gain of electron
+
+325
+00:24:46,080 --> 00:24:53,170
+إنه كسب إلكترونالسكر المختزل هو السكر بسميه مختزل
+
+326
+00:24:53,170 --> 00:25:02,030
+السكر
+
+327
+00:25:02,030 --> 00:25:05,870
+المختزل هو السكر اللذي يحتوي على functional group
+
+328
+00:25:05,870 --> 00:25:10,930
+سواء كيتون أو ألدهيد بس تكون free تكون حرة قادرة
+
+329
+00:25:10,930 --> 00:25:16,100
+على المشاركة في تفاعلات الأكسد والاختزادTamam،
+
+330
+00:25:16,100 --> 00:25:19,380
+reducing sugar هو السكر اللي بيحتوي على free
+
+331
+00:25:19,380 --> 00:25:23,860
+aldehyde او free ketone functional group يكون عنده
+
+332
+00:25:23,860 --> 00:25:28,360
+functional group free قادر على المشاركة في تفاعلات
+
+333
+00:25:28,360 --> 00:25:32,480
+الأكسدة والاختزان، لذا الشرط عشان اقول عن هذا
+
+334
+00:25:32,480 --> 00:25:36,700
+السكر ان هو reducing او non-reducingوجود free
+
+335
+00:25:36,700 --> 00:25:41,260
+aldehyde او ketone اجروا الجيت السكر هو reducing
+
+336
+00:25:41,260 --> 00:25:46,620
+sugar هو عامل مختزن يعني هو هيختزل غيره وهو
+
+337
+00:25:46,620 --> 00:25:52,770
+هيصيرله أكسداreducing sugar يعني سكر مختزل مختزل
+
+338
+00:25:52,770 --> 00:25:57,150
+يعني هو بده يختزل غيره بده يعمل اختزال لغيره و هو
+
+339
+00:25:57,150 --> 00:26:01,470
+نفسه بده يصيرله أكسده مين بده يأكسده؟ أكيد عامل
+
+340
+00:26:01,470 --> 00:26:06,270
+مؤكسد بده يجي أكسد هذا السكر و هو يصيرله أختزال
+
+341
+00:26:06,270 --> 00:26:10,010
+فعشان مكتوب sugar can be oxidized by mild
+
+342
+00:26:10,010 --> 00:26:16,850
+oxidizing agent السكر بيتأكسد بوجود عامل مؤكسد
+
+343
+00:26:17,410 --> 00:26:22,390
+والعمل المؤكسد هذا and oxidizing agent is reduced
+
+344
+00:26:22,390 --> 00:26:25,770
+in the reaction هيصير له اختزاة، اذا العامل
+
+345
+00:26:25,770 --> 00:26:31,050
+المختزل اللي هو السكر هذا بيختزل غيره، هو بيصير له
+
+346
+00:26:31,050 --> 00:26:37,150
+أكسدة، العامل المؤكسد بيأكسد غيره، هو بيصير له
+
+347
+00:26:37,150 --> 00:26:43,200
+اختزاةأجيت لما يتأكسد السكر هيتأكسد إلى acid
+
+348
+00:26:43,200 --> 00:26:47,220
+ويعطيني مركب تاني من السكريات اللي هي reducing
+
+349
+00:26:47,220 --> 00:26:50,700
+sugar بيحكي ل reducing sugar هم كل ال mono
+
+350
+00:26:50,700 --> 00:26:55,400
+-saccharide كل السكريات الأحدى دي هي عبارة عن
+
+351
+00:26:55,400 --> 00:26:59,950
+reducing sugar و ال maltose و ال lactoseكل
+
+352
+00:26:59,950 --> 00:27:04,990
+السكريات الأحادية وبالإضافة عليهم الـ maltose والـ
+
+353
+00:27:04,990 --> 00:27:08,430
+lactose من الـ disaccharide كل الـ monosaccharide
+
+354
+00:27:08,430 --> 00:27:10,950
+وعندنا الـ maltose والـ lactose من الـ
+
+355
+00:27:10,950 --> 00:27:17,420
+disaccharide هدولة بس هم يعتبروا reducing sugarزي
+
+356
+00:27:17,420 --> 00:27:20,620
+ما حكينا، مين هم الـ Oxidized اللي هم الـ Reducing
+
+357
+00:27:20,620 --> 00:27:26,080
+Sugar اللي بيقدر يصيرلهم أكسدة و هم يختزلوا غيرهم
+
+358
+00:27:26,080 --> 00:27:30,400
+كل الـ Monosaccharide و الـ Disaccharide بنعتبرهم
+
+359
+00:27:30,400 --> 00:27:35,580
+Reducing Sugar، بيصيرلهم أكسدة، لأن السكريات بتكون
+
+360
+00:27:35,580 --> 00:27:39,740
+موجودة في المحلول، بتكون على شكل خلية متوازن من
+
+361
+00:27:39,740 --> 00:27:43,940
+open chain و closed ring في منها بتكون open مفتوحة
+
+362
+00:27:43,940 --> 00:27:48,190
+خطية و في منها بتكون حلقيةكل الـ Monosaccharide
+
+363
+00:27:48,190 --> 00:27:52,150
+بتكون open و closed لكن الـ Oligosaccharide بس فيه
+
+364
+00:27:52,150 --> 00:27:55,890
+منه closed structure، only closed when cyclic
+
+365
+00:27:55,890 --> 00:27:58,690
+structure open، the aldehyde group is available
+
+366
+00:27:58,690 --> 00:28:02,290
+for oxidation مدى ما تكون عندي حلقة و أعملها open،
+
+367
+00:28:02,290 --> 00:28:05,890
+أفتحها وتصير خطية، بتكون ال functional group فيها
+
+368
+00:28:05,890 --> 00:28:10,770
+تقدر تدخل في تفاعلات الأكسدة والاختزال، يعني بتصير
+
+369
+00:28:10,770 --> 00:28:11,470
+free
+
+370
+00:28:14,480 --> 00:28:18,080
+حينها الشكل للـ Open و لـ Closed Structure of
+
+371
+00:28:18,080 --> 00:28:21,340
+Monosaccharide كيف بيكون شكلها على اليسار عندنا
+
+372
+00:28:21,340 --> 00:28:25,120
+شكل الـ Open كيف شكل الـ Linear لـ الـ Structure
+
+373
+00:28:25,120 --> 00:28:31,060
+Linear لـ السكريات في ال .. اللي على اليمين عندنا
+
+374
+00:28:31,060 --> 00:28:33,920
+الشكل الحلقى الشكل المغلق بيحكي اللي في الشكل
+
+375
+00:28:33,920 --> 00:28:38,140
+الحلقى بتكون ال .. ال functional group بتكون شفري
+
+376
+00:28:38,140 --> 00:28:42,880
+لما أفتحه أنا بيصير free functional groupبتقدر
+
+377
+00:28:42,880 --> 00:28:49,300
+تشارك في تفاعلات الأكسدا والاختزاة هنا سؤال، سؤال
+
+378
+00:28:49,300 --> 00:28:52,220
+بيحكي الـ Lactose is reducing sugar why؟ ليش الـ
+
+379
+00:28:52,220 --> 00:28:55,440
+Lactose هو يعتبر reducing sugar؟ زي ما احنا عارفين
+
+380
+00:28:55,440 --> 00:28:59,100
+ان الـ Lactose هو عبارة عن سكر ثونائي اتكون من
+
+381
+00:28:59,100 --> 00:29:04,000
+اتحاد سكر، الـ glucose و الـ galactose مع بعض،
+
+382
+00:29:04,000 --> 00:29:08,100
+برابط السكريين أجيت هدول السكريين لما يتحدوا هما
+
+383
+00:29:08,100 --> 00:29:11,020
+الاتنين، كل واحد عنده functional group من هدول
+
+384
+00:29:11,020 --> 00:29:15,630
+السكريينتحكيلي في الرابط السكرية بتيجي بتتكون في
+
+385
+00:29:15,630 --> 00:29:20,170
+هدول السكريين بين functional group من السكر الأول
+
+386
+00:29:20,170 --> 00:29:24,190
+مع موقع أخر في السكر التاني يعني في واحد من
+
+387
+00:29:24,190 --> 00:29:27,470
+السكريات بتكون الـfunctional group له مشغولة في
+
+388
+00:29:27,470 --> 00:29:31,250
+تكوين الرابط السكرية لكن السكر التاني بتكون
+
+389
+00:29:31,250 --> 00:29:34,660
+الـfunctional group عنده حرةلو جيتوا و طلعتوا على
+
+390
+00:29:34,660 --> 00:29:37,500
+تحت الـ Structure اللي تحتها ده، في عندنا رسم
+
+391
+00:29:37,500 --> 00:29:40,740
+تاني، حلق تاني، واحدة موفمان هم الرابط، الرابط
+
+392
+00:29:40,740 --> 00:29:44,000
+السكرية اللي هي رابطة التنمع بقى، لو جينا شوفنا
+
+393
+00:29:44,000 --> 00:29:47,480
+الـ Carbonyl group للحلقة الأولى اللي علي يسار،
+
+394
+00:29:47,480 --> 00:29:51,180
+عند رقم واحد هاي، هاي ال functional group، مشغولة
+
+395
+00:29:51,180 --> 00:29:54,340
+هنا، مشغولة في واش، في تكوين الرابطة مرتبطة مع
+
+396
+00:29:54,340 --> 00:29:57,770
+الحلقة التانيةلكن لو جينا للحلقة اللي عاليمين و
+
+397
+00:29:57,770 --> 00:30:00,790
+للـ Carbonyl group اللي هي الـ Carbonyl group هي
+
+398
+00:30:00,790 --> 00:30:03,790
+نفسها الـ C double bond O هي الـ functional group
+
+399
+00:30:03,790 --> 00:30:07,770
+موجودة على أقصى اليمين مش مشتركة حرة free على
+
+400
+00:30:07,770 --> 00:30:12,090
+الجنب مش مشتركة في تكوين الرفضةوالشرط عشان السكر
+
+401
+00:30:12,090 --> 00:30:17,410
+يكون reducing sugar هو وجود free carbonyl group أو
+
+402
+00:30:17,410 --> 00:30:21,650
+free functional group وهي موجودة هنا في هذا السكر
+
+403
+00:30:21,650 --> 00:30:25,010
+في اللكتوز إذا واحد من السكريات عنده free
+
+404
+00:30:25,010 --> 00:30:29,710
+functional group عشان هيك هو يعتبر reducing sugar
+
+405
+00:30:29,710 --> 00:30:33,290
+لأنه عنده free carbonyl group صحيح التاني مشغولة
+
+406
+00:30:33,290 --> 00:30:37,830
+بس المهم في واحدة free بتشارك في تفاعلات الأكسدة
+
+407
+00:30:37,830 --> 00:30:42,870
+والاختزانمن عندنا الـ non-reducing sugar؟ non
+
+408
+00:30:42,870 --> 00:30:46,230
+-reducing sugar معناها إنه not oxidized by mild
+
+409
+00:30:46,230 --> 00:30:51,150
+oxidizing agent يعني مش هيصيرلهم أكسدة بوجود عامل
+
+410
+00:30:51,150 --> 00:30:55,030
+مؤكسد، لا هم هيختزلوا غيرهم ولا هم يصيرلهم أكسدة،
+
+411
+00:30:55,030 --> 00:31:00,110
+من هم؟ هم الساكروز وكل ال polysaccharides الساكروز
+
+412
+00:31:00,110 --> 00:31:04,370
+وكل ال polysaccharides يعتبروا non-reducing sugar
+
+413
+00:31:05,600 --> 00:31:09,620
+طيب الساكروز is not reducing sugar, why؟ ليش
+
+414
+00:31:09,620 --> 00:31:13,900
+الساكروز مش reducing sugar؟ بيحكيلي الساكروز هو
+
+415
+00:31:13,900 --> 00:31:19,700
+سكر ثونائي عبارة عن اتحاد سكر الجولكوز والفراكتوز
+
+416
+00:31:19,700 --> 00:31:24,020
+اتحاد سكر الجولكوز بالفراكتوزبحكي لما ييجوا هدول
+
+417
+00:31:24,020 --> 00:31:27,560
+السكريين يتحدى عشان يكونوا للـSaccharose بيرتبطوا
+
+418
+00:31:27,560 --> 00:31:30,480
+مع بعض وبتيجى الرابط السكرية بينهم بين
+
+419
+00:31:30,480 --> 00:31:33,740
+الـfunctional group للسكر الأول والـfunctional
+
+420
+00:31:33,740 --> 00:31:37,220
+group للسكر التاني، اذا الرابط السكرية ده بتيجى
+
+421
+00:31:37,220 --> 00:31:40,680
+بين الـfunctional group للسكر الأول والـfunctional
+
+422
+00:31:40,680 --> 00:31:44,140
+group للسكر التاني، يعني الكربونيلي group من السكر
+
+423
+00:31:44,140 --> 00:31:48,480
+الأول بتيجى مع الكربونيلي group من السكر التانيفما
+
+424
+00:31:48,480 --> 00:31:51,000
+انهم مرتبطوا من عند الـ functional group في
+
+425
+00:31:51,000 --> 00:31:55,380
+الحالتين يبقى فيش free functional group وبالتالي
+
+426
+00:31:55,380 --> 00:31:59,300
+بتكون الـ carbonyl group are busy in the two sides
+
+427
+00:31:59,300 --> 00:32:04,200
+بتكون مشغولة في الجهتين ففيش free functional group
+
+428
+00:32:04,470 --> 00:32:09,410
+تشارك في تفاعلات الأكسد والاختزاق فالسكراوز not
+
+429
+00:32:09,410 --> 00:32:13,010
+reducing sugar لأنه اختل شرط أن يكون reducing
+
+430
+00:32:13,010 --> 00:32:17,190
+sugar وهو وجود free functional group مافيش عنده
+
+431
+00:32:17,190 --> 00:32:20,170
+free functional group، they are busy in the two
+
+432
+00:32:20,170 --> 00:32:24,410
+sides، في السكريين مشغولة في تكوين الرابط السكرية
+
+433
+00:32:26,160 --> 00:32:30,680
+إيش هي ال oxidizing agent اللي ممكن استخدمها لكشف
+
+434
+00:32:30,680 --> 00:32:35,920
+عن وجود reducing sugar لازم يكون عنده سكر بيختزل
+
+435
+00:32:35,920 --> 00:32:39,520
+غيره و عامل مواكسيد عشان هو ي access ال sugar مين
+
+436
+00:32:39,520 --> 00:32:42,300
+هم العوامل الواكسيدة اللي ممكن استخدمها؟ هي
+
+437
+00:32:42,300 --> 00:32:47,780
+البنديكسولوشن، فيه فيلنكسولوشن، طولنزرياجن و بيكرك
+
+438
+00:32:47,780 --> 00:32:53,260
+أسدسولوشنBendects, Filling, Tolens, Bacaric Acid
+
+439
+00:32:53,260 --> 00:32:57,480
+إيش هو الـ Oxidizing Agent؟ الـ Oxidizing Agent هو
+
+440
+00:32:57,480 --> 00:33:01,680
+العامل اللي اسمه تاني Oxidizer or Oxidant العامل
+
+441
+00:33:01,680 --> 00:33:06,380
+اللي بيأكسد غيره هو نفسه بيختزن تعريفه Chemical
+
+442
+00:33:06,380 --> 00:33:10,920
+Compound That Transfers Oxygen Atom هو مركب كيميا
+
+443
+00:33:10,920 --> 00:33:16,740
+ينقل ضرر أكسجين أو هو اللي بيكسب إلكترون إلكترون
+
+444
+00:33:16,740 --> 00:33:21,710
+in Redox chemical reactionهدأ الـ reduction طيب
+
+445
+00:33:21,710 --> 00:33:24,490
+الألدوز اللي جيت احنا لما يصير عندنا oxidation
+
+446
+00:33:24,490 --> 00:33:28,030
+reduction reaction السكر reducing sugar ليش هيتحول
+
+447
+00:33:28,030 --> 00:33:31,610
+لما يصير له oxidation؟ لأن السكر هو عامل مختزل،
+
+448
+00:33:31,610 --> 00:33:35,790
+بدي يصير له أكسدة، فالسكر هيختزل إلى أيه؟ هيتأكسد
+
+449
+00:33:35,790 --> 00:33:39,490
+إلى أيش؟ إذا كان السكر نوع ألدوز عنده aldehyde
+
+450
+00:33:39,490 --> 00:33:42,830
+group زي الجوليكوز، لما يصير له oxidation، بتحول
+
+451
+00:33:42,830 --> 00:33:47,770
+إلى حاجة اسمها carboxylateأما إذا كان السكر نوعه
+
+452
+00:33:47,770 --> 00:33:52,190
+كيتوز عنده كيتوني جروب زي الفركتوز مايصير
+
+453
+00:33:52,190 --> 00:33:56,810
+oxidation بتحول لحاجة اسمها hydroxylcarboxylate
+
diff --git a/PL9fwy3NUQKwZ1Q7zOg1ObgufPMjRI3WY1/MUWHefMMBZ4_raw.srt b/PL9fwy3NUQKwZ1Q7zOg1ObgufPMjRI3WY1/MUWHefMMBZ4_raw.srt
new file mode 100644
index 0000000000000000000000000000000000000000..8dbaf45588d427ab9ae6f7c9d7d24401d0fdc3fc
--- /dev/null
+++ b/PL9fwy3NUQKwZ1Q7zOg1ObgufPMjRI3WY1/MUWHefMMBZ4_raw.srt
@@ -0,0 +1,1812 @@
+1
+00:00:00,220 --> 00:00:03,140
+عملنا qualitative analytical test for
+
+2
+00:00:03,140 --> 00:00:08,500
+carbohydrates اللي هي ما عملنا بيحكي عن أنه فحوصات
+
+3
+00:00:08,500 --> 00:00:14,360
+نوعية فحوصات جودة للكشف عن ال carbohydrates عن
+
+4
+00:00:14,360 --> 00:00:18,800
+السكريات اليوم فحوصاتنا للكشف عن ال carbohydrate
+
+5
+00:00:18,800 --> 00:00:23,180
+وهي فحوصات qualitative فحوصات جودة يعني بيطلع
+
+6
+00:00:23,180 --> 00:00:26,940
+معايا في الآخر لون هيكون positive أو negative مش
+
+7
+00:00:26,940 --> 00:00:32,480
+هيطلع معايا كمية أو رقمماشي أهداف معملنا اليوم
+
+8
+00:00:32,480 --> 00:00:35,660
+بشكل عام المعمل كله بدنا نطلع منه general
+
+9
+00:00:35,660 --> 00:00:38,760
+information about carbohydrates بدنا نطلع منه
+
+10
+00:00:38,760 --> 00:00:41,740
+بمعلومات عامة عن الكربوهيدرات نعرف شو هي
+
+11
+00:00:41,740 --> 00:00:44,840
+الكربوهيدرات differentiate between carbohydrates
+
+12
+00:00:44,840 --> 00:00:49,420
+أني بدي أقارن .. بدي أفرد من أنواع الكربوهيدرات
+
+13
+00:00:49,420 --> 00:00:53,320
+أنواع السكريات qualitative analysis of
+
+14
+00:00:53,320 --> 00:00:57,000
+carbohydrates بعدين نشوف الفحوصات أو الجزء العملي
+
+15
+00:00:57,000 --> 00:01:02,240
+اللي هو فحوصات qualitativeللكشف عن السكريات
+
+16
+00:01:03,510 --> 00:01:06,930
+Equalitative Test of Amino Acids Equalitative
+
+17
+00:01:06,930 --> 00:01:10,790
+Analytical Test إيش هي qualitative؟ it is
+
+18
+00:01:10,790 --> 00:01:13,810
+concerned with determining the identity of a
+
+19
+00:01:13,810 --> 00:01:17,930
+substance لمعرفة هوية هذه المادة بس بدي أعرف هل
+
+20
+00:01:17,930 --> 00:01:21,050
+هذه المادة موجودة أو لا مثلا سكر موجود أو لا
+
+21
+00:01:21,050 --> 00:01:25,710
+enable us to detect the presence of things برضه
+
+22
+00:01:25,710 --> 00:01:30,230
+نفس الكلام إنه أعرف هل هذه الموجودة الأشياء أو لا
+
+23
+00:01:30,950 --> 00:01:37,030
+بحوصاتك qualitative يعني موجود أو غير موجود بداية
+
+24
+00:01:37,030 --> 00:01:40,330
+بدنا نتعرف على ال carbohydrates على السكرية
+
+25
+00:01:40,330 --> 00:01:45,070
+السكرية تم قسم إلى قسمين في منها simple saccharide
+
+26
+00:01:45,070 --> 00:01:47,930
+هي السكرية يعني simple سكرية وفي منها
+
+27
+00:01:47,930 --> 00:01:53,060
+polysaccharide سكرية بسيطة وسكرية تعدينةفي البداية
+
+28
+00:01:53,060 --> 00:01:56,020
+نيجي نحكي عن الـ simple saccharide الـ saccharide
+
+29
+00:01:56,020 --> 00:02:00,420
+هي من خصائصها إنه إلها low molecular weight الـ
+
+30
+00:02:00,420 --> 00:02:03,780
+molecular weight الوزن الجزائي إلها قليل وكمان هي
+
+31
+00:02:03,780 --> 00:02:08,600
+soluble in water بتتوق في الميه وكمان هي sweet to
+
+32
+00:02:08,600 --> 00:02:13,840
+طارس طعمها حلو إذا الـ saccharide الوزن الجزائي
+
+33
+00:02:13,840 --> 00:02:19,310
+إلها قليل ذائبة في الماء وطعمها حلوبتنقسم إلى ..
+
+34
+00:02:19,310 --> 00:02:23,170
+أنا مكتبت قسمياً هما تلاتة في منها mono-saccharide
+
+35
+00:02:23,170 --> 00:02:28,750
+سكريات أحادية في di-saccharide سكريات ثنائية في
+
+36
+00:02:28,750 --> 00:02:33,990
+oligosaccharide ألاقيت mono-saccharide سكر واحد،
+
+37
+00:02:33,990 --> 00:02:37,250
+دي بس واحد، سكر واحد، simple sugar di-saccharide
+
+38
+00:02:37,250 --> 00:02:40,970
+هي double sugar، بيكون سكر ثنائي اتنين من الـ mono
+
+39
+00:02:40,970 --> 00:02:45,070
+بيتحدوا مع بعض oligo بتكون من تلاتة إلى تسعة، عدد
+
+40
+00:02:45,070 --> 00:02:49,480
+السكريات فيها من تلاتة إلى تسعةالـ Monosaccharide
+
+41
+00:02:49,480 --> 00:02:54,140
+هي simple sugar هو سكر واحد بيكون الصيغة العامة له
+
+42
+00:02:54,140 --> 00:03:04,170
+CH2O لكل N N هي عدد ذرات الكعكصيغة له CH2O كل N N
+
+43
+00:03:04,170 --> 00:03:07,670
+هي عدد الذرات الكربون مثال عليها الـ
+
+44
+00:03:07,670 --> 00:03:12,330
+glyceraldehyde وهذا الـ glyceraldehyde فيه 3 ذرات
+
+45
+00:03:12,330 --> 00:03:18,390
+كربون فبتكون الصيغة العامة له C3H6O3
+
+46
+00:03:19,600 --> 00:03:24,400
+في عندنا جيليكوز، فراكتوز، جالاكتوز، هدولة mono
+
+47
+00:03:24,400 --> 00:03:29,120
+-saccharide، سكريات إحادية، hexose بنسميها هم
+
+48
+00:03:29,120 --> 00:03:32,860
+التلاتة الجيليكوز و فراكتوز و جالاكتوز، بنسميهم
+
+49
+00:03:32,860 --> 00:03:36,720
+hexose، سكرية simple-saccharide، mono-saccharide،
+
+50
+00:03:36,720 --> 00:03:41,460
+ولكن سوداسي، عدد ذرّات الكربون فيه 6في عندنا الـ
+
+51
+00:03:41,460 --> 00:03:46,080
+ribose و الـ manose و الـ xylose و الـ arabinose
+
+52
+00:03:46,080 --> 00:03:50,860
+هدولة فيهم خمس ذرات كربون بنطلق عليهم باسم Pentose
+
+53
+00:03:50,860 --> 00:03:55,340
+لذالن الـ monosaccharide هي simple sugar فيها سكر
+
+54
+00:03:55,340 --> 00:04:00,880
+واحد الصيغة العامة يلها CH2 all كل M أمثل عليها
+
+55
+00:04:00,880 --> 00:04:04,580
+اللي هو الـ glyceraldehyde بتكون من تلت ذرات كربون
+
+56
+00:04:05,010 --> 00:04:08,970
+ويعنى Gelicose وFructose وGalactose هدولة هكسوز
+
+57
+00:04:08,970 --> 00:04:13,630
+وكريات فيها 6 ذرات كاربوم نسميها هكسوز فيعنى
+
+58
+00:04:13,630 --> 00:04:20,330
+Ribose ومالنوز Ribose ومالنوز زيلوز أرابينوز هدولة
+
+59
+00:04:20,330 --> 00:04:26,280
+خماسي نسميها Pentoseأما الـ Disaccharide هو Double
+
+60
+00:04:26,280 --> 00:04:29,960
+Sugar سكة تنائية بتكون من اتنين Monosaccharide
+
+61
+00:04:29,960 --> 00:04:37,580
+بيتتحدوا مع بعض الصيغة العامة له C12H22O11
+
+62
+00:04:37,580 --> 00:04:42,980
+هي سكرين تنائيين بيجوا بيتتحدوا مع بعض بتكون من ست
+
+63
+00:04:42,980 --> 00:04:46,980
+ذرات كاربون يعني الصيغة أصلاً لكل واحد منهم هي
+
+64
+00:04:46,980 --> 00:04:49,700
+C12H
+
+65
+00:04:53,840 --> 00:04:57,420
+C6H12O6 إذن الـ «تايسا كرايد» هو اتحاد سكريين
+
+66
+00:04:57,420 --> 00:05:01,340
+تنائيين عبارة عن «هيكسوز» عبارة عن «سوداسي» C6 للـ
+
+67
+00:05:01,340 --> 00:05:04,920
+«رات كاربون» يعني الصيغة لكل واحد منهم من هدول
+
+68
+00:05:04,920 --> 00:05:11,200
+الوحيد اللي قاشوا اتحدوا مع بعض هي «C6H12O6»
+
+69
+00:05:11,880 --> 00:05:17,200
+يجي اتنين مع بعض C6 H12 O6 زائد C6 H12 O6 بيتحدوا
+
+70
+00:05:17,200 --> 00:05:22,520
+مع بعض افترض يعطيني Dice Caride سيغته العامة C12
+
+71
+00:05:22,520 --> 00:05:28,880
+H24 O12 طب هنا مكتوب ان الـ Dice Caride السيغة
+
+72
+00:05:28,880 --> 00:05:36,810
+العامة له C12 H22 O11موين راحت؟ ذرتين هيدروجين
+
+73
+00:05:36,810 --> 00:05:41,770
+وذرة أكسوجين مفقودين مفقودين في تكوين الرابط
+
+74
+00:05:41,770 --> 00:05:48,110
+السكرية بين السكريان والوحديان ارتباط سكريان
+
+75
+00:05:48,110 --> 00:05:53,130
+ووحديان مع بعض عشان يكونوا السكر التنائي بيرتبطوا
+
+76
+00:05:53,130 --> 00:05:59,080
+مع بعض برابط سكريةاسمها glycosidic bond هاي الـ
+
+77
+00:05:59,080 --> 00:06:05,840
+glycosidic point عندما تتكون ينتج عنها جزء ماء،
+
+78
+00:06:05,840 --> 00:06:11,380
+نزع جزء ماء فاسم التفاعل اللي بينتج عنه نزع جزء
+
+79
+00:06:11,380 --> 00:06:17,200
+ماء هو Condensation Reaction نفس التفاعل تكون
+
+80
+00:06:17,200 --> 00:06:21,470
+الرابط الـ PPD اللي حكينا عنهفعشان هي صيغة
+
+81
+00:06:21,470 --> 00:06:27,410
+السكريات السونية صارت C12H22O11
+
+82
+00:06:27,410 --> 00:06:32,610
+ونزعنا جزء ماء لتكوين الرابط السكرية اللي اسمها
+
+83
+00:06:32,610 --> 00:06:36,760
+glycosidic bondطيب، إيش الأمثلة على الـ
+
+84
+00:06:36,760 --> 00:06:41,400
+Disaccharide؟ عندنا Maltose، Saccharose و Lactose،
+
+85
+00:06:41,400 --> 00:06:45,680
+هدول سكريات و نايم حكينا إن هما عبارة عن اتحاد
+
+86
+00:06:45,680 --> 00:06:49,900
+سكريين أحدين مع بعض لو جينا حكينا عن الـ Maltose،
+
+87
+00:06:49,900 --> 00:06:53,680
+الـ Maltose هو عبارة عن اتحاد جليكوز مع جليكوز،
+
+88
+00:06:53,680 --> 00:06:57,900
+بيتحدوا مع بعض، بيكونولي Maltose الـ Saccharose هو
+
+89
+00:06:57,900 --> 00:07:03,060
+عبارة عن اتحاد جليكوز مع Efractoseالـ Lactose هو
+
+90
+00:07:03,060 --> 00:07:09,080
+عبارة عن اتحاد جليكوز مع Galactose لذا الملتوز
+
+91
+00:07:09,080 --> 00:07:14,440
+جليكوز و جليكوز السكاروز جليكوز و فراكتوز اللاكتوز
+
+92
+00:07:14,440 --> 00:07:19,300
+هو جليكوز و Galactose والـ Oligo حكينا عنها أنها
+
+93
+00:07:19,300 --> 00:07:24,080
+بتتكون من 3 إلى 9 سكريات مع بعض أما بالنسبة لـ
+
+94
+00:07:24,080 --> 00:07:27,920
+Polysaccharide اللي هي عديدة التسكر هي Complex
+
+95
+00:07:27,920 --> 00:07:34,330
+Sugar هي سكريات معقدةمن خصائصها إنه إلها high
+
+96
+00:07:34,330 --> 00:07:38,790
+molecular weight الوزن الجزائي لها عالي Insoluble
+
+97
+00:07:38,790 --> 00:07:42,350
+in water ما بتدوب في الماء لأنها عبارة عن سنسلة
+
+98
+00:07:42,350 --> 00:07:47,670
+طويلة بتفرعات فبيكون Insoluble كمان هي tasteless
+
+99
+00:07:47,670 --> 00:07:53,240
+لا طعم لها حكينا إنه هي multiple sugarتكون فيها
+
+100
+00:07:53,240 --> 00:07:57,740
+تفارع صيغة العامة ليها غير مطلوبة أمثلة عليها هي
+
+101
+00:07:57,740 --> 00:08:02,460
+الـ starch اللي هو النشا النباتي و الـ glycogen هو
+
+102
+00:08:02,460 --> 00:08:07,020
+النشا الحيواني الـ cellulose اللي بتستخدم النبات
+
+103
+00:08:07,020 --> 00:08:11,660
+لبناء الـcell wallاللينن والكايتن اللي بتستخدم
+
+104
+00:08:11,660 --> 00:08:15,620
+الحشرات لبناء ال cell wall ده من الأمثل على ال
+
+105
+00:08:15,620 --> 00:08:20,320
+polysaccharide star جناش النباتي وجلايكوجن نشا
+
+106
+00:08:20,320 --> 00:08:24,340
+حيواني cellulose اللي بيستخدم في النبات لبناء ال
+
+107
+00:08:24,340 --> 00:08:29,640
+cell wall اللينن والكايتن اللي بيستخدم في الحشرات
+
+108
+00:08:29,640 --> 00:08:34,900
+لبناء ال cell wallبينهاج اسمها الـ functional
+
+109
+00:08:34,900 --> 00:08:39,520
+group functional group هي منطقة في المُركّب تحدد
+
+110
+00:08:39,520 --> 00:08:43,720
+خصائصه هي region of molecules that give it a
+
+111
+00:08:43,720 --> 00:08:48,860
+particular property single molecule can have more
+
+112
+00:08:48,860 --> 00:08:51,620
+than one functional group as parts of its
+
+113
+00:08:51,620 --> 00:08:55,380
+structure ممكن يكون عنده أكتر من functional group
+
+114
+00:08:56,660 --> 00:08:59,560
+الـ active part أو الـ functional group اللي
+
+115
+00:08:59,560 --> 00:09:04,620
+موجودة في السكريات هي الديهيد أو كيتون group بتكون
+
+116
+00:09:04,620 --> 00:09:09,540
+الديهيد أو كيتون الديهيد أو كيتون and هيدروكسيل
+
+117
+00:09:09,540 --> 00:09:13,980
+group لذلك اللي موجود بيكون الديهيد أو كيتون أو
+
+118
+00:09:13,980 --> 00:09:16,700
+هيدروكسيل group هذا اللي بيكونوا موجودين في
+
+119
+00:09:16,700 --> 00:09:21,800
+السكريات هي الـ functional groupطلّع تحت إيش الفرق
+
+120
+00:09:21,800 --> 00:09:25,440
+بين الـ Aldehyde وإيش الفرق بينه وبين الـ Ketone
+
+121
+00:09:25,440 --> 00:09:31,620
+الـ Aldehyde بتكون عندنا C مرتبطة مع الـ double
+
+122
+00:09:31,620 --> 00:09:37,540
+bond A من جهة مرتبطة بذرّة هيدروجين ومن الجهة
+
+123
+00:09:37,540 --> 00:09:42,510
+التانية مرتبطة بـ R group، بمجموعةإذاً في الـ
+
+124
+00:09:42,510 --> 00:09:47,230
+aldehyde بتكون عنا الـ C double bond O مرتبطة من
+
+125
+00:09:47,230 --> 00:09:53,150
+جهة بذرة هيدروجين ومن جهة تانية مرتبطة بـ R group
+
+126
+00:09:53,150 --> 00:09:57,470
+مجموعة مش بذرة هيدروجين بسيطة فقط وإنما بمجموعة
+
+127
+00:09:57,470 --> 00:10:00,970
+إذا لاجينا الـ C double bond O من جهة مرتبطة بذرة
+
+128
+00:10:00,970 --> 00:10:04,950
+هيدروجين ومن جهة تانية بمجموعة بـ R group بسميها
+
+129
+00:10:04,950 --> 00:10:10,330
+aldehyde أما إذا شوفت الـ C double bond Oمرتبطة من
+
+130
+00:10:10,330 --> 00:10:14,690
+جهة بـ R-group مجموعة ومن جهة التانية كمان R-group
+
+131
+00:10:14,690 --> 00:10:19,930
+مجموعة بسميها كيتون لذن الـ aldehyde بتكون الـCWO
+
+132
+00:10:19,930 --> 00:10:24,970
+مرتبطة من جهة بـH ومن جهة بـR أما الكيتون بتكون
+
+133
+00:10:24,970 --> 00:10:31,990
+الـCWO مرتبطة من جهة بـR ومن جهة بـR تاني زي تحت
+
+134
+00:10:31,990 --> 00:10:35,410
+مثل عن الـGlucose و الـFructoseلو جينا تلالنا على
+
+135
+00:10:35,410 --> 00:10:38,190
+الـ Structure للـ Glycose نيجي نطلع الـ C double
+
+136
+00:10:38,190 --> 00:10:42,290
+bond O وينها، هي على الشمال، C double bond O محوطة
+
+137
+00:10:42,290 --> 00:10:47,450
+عليها، إيش مرتبطة من جهة مرتبطة بضارة هيدروجين؟ من
+
+138
+00:10:47,450 --> 00:10:51,610
+الجهة التانية مرتبطة بمجموعة، ب R group، اللي هي C
+
+139
+00:10:51,610 --> 00:10:57,640
+-O-H-Hإذا من جهة مرتبطة بـ H ومن جهة بـ R مين يبقى
+
+140
+00:10:57,640 --> 00:11:01,500
+هيكون Aldehyde ولا كيتون الجليكوز؟ أكيد هيكون
+
+141
+00:11:01,500 --> 00:11:05,360
+Aldehyde لأنه مرتبطة C double bond O من جهة بـ R
+
+142
+00:11:05,360 --> 00:11:10,060
+ومن جهة بـ H لو جينا للفركتوز مكتوب بالناس بال
+
+143
+00:11:10,060 --> 00:11:13,260
+structure C double bond O اللي هي محوط عليها من
+
+144
+00:11:13,260 --> 00:11:19,130
+جهة مرتبطةبـ R group اللي هي CHHOH من الجهة
+
+145
+00:11:19,130 --> 00:11:23,650
+التانية مرتبطة بـ CHOH برضه R تانية إذا الـ C
+
+146
+00:11:23,650 --> 00:11:27,630
+double bond O في الفركتوز متصلة من الجهتين بـ R من
+
+147
+00:11:27,630 --> 00:11:33,250
+جهة و R من جهة يبقى هتكون كيتونتمام؟ إذا الجليكوز
+
+148
+00:11:33,250 --> 00:11:37,850
+عبارة عن الديهايد والفركتوز عبارة عن كيتون أصبحنا
+
+149
+00:11:37,850 --> 00:11:42,230
+عارفين إيش الفرق بين الالديهايد وكيتون بحكينا إن
+
+150
+00:11:42,230 --> 00:11:47,970
+الالديهايد بتكون على كربونا رقم 1 والكيتون بيكون
+
+151
+00:11:47,970 --> 00:11:52,170
+على كربونا رقم 2 إنه بدي أرقم تقول لو جينا على
+
+152
+00:11:52,170 --> 00:11:55,330
+الجليكوز نيجي الرقم من الكربون نبدأ الترقيم من
+
+153
+00:11:55,330 --> 00:12:00,850
+الجهة الأقرب للـCW1الترقيم يبدأ من الجهة الأقرب
+
+154
+00:12:00,850 --> 00:12:04,730
+للـ CW من ضهر هذه الجهة من عند الـ functional
+
+155
+00:12:04,730 --> 00:12:07,390
+group من الجهة الأقرب للـ functional group من عند
+
+156
+00:12:07,390 --> 00:12:10,770
+الـ CW من ضهر هتطلع كربونا رقم واحد ومتصلة من جهة
+
+157
+00:12:10,770 --> 00:12:15,110
+الـ H ومن جهة المجموعة فالالدهيد في الجلوكوز الـ
+
+158
+00:12:15,110 --> 00:12:19,540
+CW من ضهر موجود على رقم كربونا رقم واحدلو جينا على
+
+159
+00:12:19,540 --> 00:12:22,760
+الـ Fructose جينا الرقم من اليسارها، الرقم هي في
+
+160
+00:12:22,760 --> 00:12:26,580
+عندي كربونا رقم 1، وبعدين 2 كربونا اللي هي C
+
+161
+00:12:26,580 --> 00:12:31,440
+double bond 1 في الـ Ketone، في الـ Fructose اللي
+
+162
+00:12:31,440 --> 00:12:37,860
+هي موجودة على كربونا رقم 2هنا حاجة اسمها Sugar
+
+163
+00:12:37,860 --> 00:12:43,560
+Nomenclature اللي هي السكر وصورته كأنه انعكسه في
+
+164
+00:12:43,560 --> 00:12:47,700
+المرايا كأنه mirror image أن السكر له انعكاس نفس
+
+165
+00:12:47,700 --> 00:12:53,820
+السكر موجود على شكلين في منه الـD وأقل بنسميهم
+
+166
+00:12:53,820 --> 00:12:56,760
+يعني مثلا الـGelicose في منه الـD-Gelicose وأقل
+
+167
+00:12:56,760 --> 00:13:00,940
+الـGelicose كيف هذا بدي أقرر هل هو الـD ولا الأقل؟
+
+168
+00:13:01,480 --> 00:13:05,080
+لما نكتب السكر الـ structure له بشكل linear بشكل
+
+169
+00:13:05,080 --> 00:13:10,540
+خطي بشكل عمودي بدي أرقم الـ carbon بيطلع على
+
+170
+00:13:10,540 --> 00:13:18,020
+carbonة رقم 5 بيطلع على carbonة رقم 5 إذا الـ OH
+
+171
+00:13:18,020 --> 00:13:23,560
+موجودة إلى جهة الـ left إلى جهة اليسار بيكون الـ 4
+
+172
+00:13:24,460 --> 00:13:29,280
+دي هتطلع على كربونا رقم 5 فنطلع على الـ Hydroxyl
+
+173
+00:13:29,280 --> 00:13:32,160
+group اللي متصلة فيها اللي هي الـ OH اللي متصلة
+
+174
+00:13:32,160 --> 00:13:37,020
+فيها إذا كانت على جهة الـ Left بيكون Alpha إذا
+
+175
+00:13:37,020 --> 00:13:41,900
+كانت الـ Hydroxyl group على كربونا 5 إلى جهة الـ
+
+176
+00:13:41,900 --> 00:13:47,390
+Right إلى جهة اليمينبكون «DF»، إذاً بدي أحدد هل هو
+
+177
+00:13:47,390 --> 00:13:53,210
+«D» ولا «L» برقم الكربونة، ببطل على كربونة رقم 5،
+
+178
+00:13:53,210 --> 00:13:56,150
+إذا الـ «OH» الـ hydroxyl group المتصلة فيها كانت
+
+179
+00:13:56,150 --> 00:14:00,010
+إلى جهة الـ «Left»، بتكون «Alpha»، إذا كانت إلى
+
+180
+00:14:00,010 --> 00:14:05,560
+جهة ال «Right»، بيكون «DF»بحكي لي most naturally
+
+181
+00:14:05,560 --> 00:14:09,720
+occurring sugars are D isomers إن معظم السكريات في
+
+182
+00:14:09,720 --> 00:14:14,020
+الجسم هي على شكل الـD form لكن الأحمض الأمنية في
+
+183
+00:14:14,020 --> 00:14:18,300
+الجسم موجودة على شكل الـForm لو جينا مثلا على هاي
+
+184
+00:14:18,300 --> 00:14:20,640
+الرسم، على الـStructure اللي على جانبها الجليكوز
+
+185
+00:14:20,640 --> 00:14:24,020
+نيجي نرقم، حكينا من وين نبدأ ترقيم، من عند
+
+186
+00:14:24,020 --> 00:14:26,880
+الـfunctional group يعني الـD الجليكوز، هذا اللي
+
+187
+00:14:26,880 --> 00:14:30,160
+مكتوب عليه D جليكوز كيف حكينا إنه D؟ كيف حددوا إنه
+
+188
+00:14:30,160 --> 00:14:33,740
+D؟بدي أجي أرقم من وين أبدأ الترقيم من تحت و لا من
+
+189
+00:14:33,740 --> 00:14:36,760
+فوق من عند الـ functional group اللي هي الـ C
+
+190
+00:14:36,760 --> 00:14:40,960
+double bond هو وين موجودة أقرب، فوق و لا تحت، فوق،
+
+191
+00:14:40,960 --> 00:14:45,440
+لذا هابدأ الترقيم من فوق عند Carbon رقم 1، 2، 3،
+
+192
+00:14:45,440 --> 00:14:50,200
+4، 5 عند Carbon رقم 5 اللي هي اللي جنبها OH معلمة
+
+193
+00:14:50,200 --> 00:14:53,660
+بالأصفر عند هذه الـ Carbon هبدأ أطلع على الـ OH
+
+194
+00:14:53,660 --> 00:14:56,520
+اللي هي الـ hydroxyl group هل موجودة إلى الـ left
+
+195
+00:14:56,520 --> 00:14:59,990
+ولا إلى ال right؟ إلى اليمين ولا للشمال؟موجودة على
+
+196
+00:14:59,990 --> 00:15:04,750
+اليمين إذا هو D form إذا هو D يجي لكاذب نيجي
+
+197
+00:15:04,750 --> 00:15:08,510
+للتاني بدي أرقم برضه من الجهة الأقرب لل functional
+
+198
+00:15:08,510 --> 00:15:12,290
+group اللي هي ال four برقم ال carbon واحد، اتنين،
+
+199
+00:15:12,290 --> 00:15:15,870
+تلاتة، أربعة، خمسة ال carbon الرقم خمسة اللي هي
+
+200
+00:15:15,870 --> 00:15:19,430
+معلمة بالأصفر وهي عندنا ال OH هل هي إلى ال left
+
+201
+00:15:19,430 --> 00:15:25,570
+ولا إلى ال right؟ لل left إذا هندقها left إذا هو
+
+202
+00:15:25,570 --> 00:15:31,380
+ال fourفي عندنا السُكر لما بدي أرسم الـ structure
+
+203
+00:15:31,380 --> 00:15:35,580
+للسُكريات في منها شكل إيه؟ في منها شكل Linear خطي
+
+204
+00:15:35,580 --> 00:15:41,040
+عمودي بيكون وفي منها شكل حلقة هلجيت الشكل الـ
+
+205
+00:15:41,040 --> 00:15:44,180
+Linear اللي عندى؟ حكينا بنقدر نكتب عليه هو الـ D
+
+206
+00:15:44,180 --> 00:15:47,720
+أو R هلجيت لو عند الـ Linear هذا و بتعمل منه حلقة
+
+207
+00:15:47,720 --> 00:15:54,590
+كيف؟ بربط Carbon رقم 5 مع الـ functional groupكيف
+
+208
+00:15:54,590 --> 00:16:00,070
+بدأ أحول من Linear أو عمودي لمغلق برابط Carbon رقم
+
+209
+00:16:00,070 --> 00:16:03,330
+5 مع الـ Functional Group ألقيت عندي حلقتين
+
+210
+00:16:03,330 --> 00:16:07,070
+هايومها، احكيلي الحلقة لما نسميها، بنسميها Alpha
+
+211
+00:16:07,070 --> 00:16:10,490
+أو Beta، هل هي Alpha أو Beta كيف بدها تكون Alpha
+
+212
+00:16:10,490 --> 00:16:16,490
+أو Beta، كيف بدأ أميز هل هي Alpha أو Beta؟بحكي ليه
+
+213
+00:16:16,490 --> 00:16:21,970
+بنطلع على Carbona رقم 1؟ أشوف الـ OH المتصل فيها،
+
+214
+00:16:21,970 --> 00:16:26,130
+هل هي إلى الأسفل أو للأعلى؟ إذا الـ OH المتصلة مع
+
+215
+00:16:26,130 --> 00:16:30,870
+Carbona رقم 1 كانت below، كانت للأسفل، أسفل
+
+216
+00:16:30,870 --> 00:16:36,060
+الحلقة، بنسميها Alphaإذا كانت الـ OH متصلة في
+
+217
+00:16:36,060 --> 00:16:40,880
+الكربونة رقم 1 للأعلى، بسميه Beta يعني لو جينا
+
+218
+00:16:40,880 --> 00:16:45,640
+الحلقتين الموجودين عندنا، بنرقم الكربون عند كل
+
+219
+00:16:45,640 --> 00:16:48,580
+نقطة، عند كل زاوية، بيكون فيها ذرّة كربون في
+
+220
+00:16:48,580 --> 00:16:52,120
+الحلقة هلجت هذه الكربون، بدي أرقمها، من أين نبدأ
+
+221
+00:16:52,120 --> 00:16:55,340
+ترقيم حكينا؟ من عند الـ functional groupكيف تتعرف
+
+222
+00:16:55,340 --> 00:16:57,700
+فى الـ functional group فى الحلقة؟ بتكون مرتبطة فى
+
+223
+00:16:57,700 --> 00:17:00,500
+الـA C double bond A مش هي بتكون مرتبطة فى الـA
+
+224
+00:17:00,500 --> 00:17:04,540
+فهى الـA هى الكربونة المتصلة فىها هي كربونة رقم 1
+
+225
+00:17:04,540 --> 00:17:10,860
+برقم 1,2,3,4,5,6 مركزين معايا على الحلقة الأولى
+
+226
+00:17:10,860 --> 00:17:15,430
+اللى علي الشمال أول حلقة هي مرقمةمن عند الـ
+
+227
+00:17:15,430 --> 00:17:20,770
+Function Algorithm 1,2,3,4,5,6 كربونة رقم 1 اللي
+
+228
+00:17:20,770 --> 00:17:24,690
+قد تطلع عليها When الـ OH هي بالأزرق الـ OH هل هي
+
+229
+00:17:24,690 --> 00:17:28,810
+للأسفل ولا للأعلى؟ للأسفل، إذن هو Alpha-Glucose
+
+230
+00:17:29,520 --> 00:17:33,320
+جينا على الحلقة التانية، بدي أرقم الـ Carbon، من
+
+231
+00:17:33,320 --> 00:17:36,500
+وين أرقم؟ من عند الـ Functional Group، واحد،
+
+232
+00:17:36,500 --> 00:17:41,000
+اتنين، تلاتة، اربعة، خمسة، ستة، هجد أشوف الـ OH
+
+233
+00:17:41,000 --> 00:17:44,540
+المتصلة في Carbon رقم واحد، هيو معلم ليها بالازرق
+
+234
+00:17:45,010 --> 00:17:49,150
+وين موجودة للأعلى أو للأسفل؟ موجودة الـ OH للأعلى
+
+235
+00:17:49,150 --> 00:17:53,550
+above the ring إذا هو Beta Glucose إذا لما بدي
+
+236
+00:17:53,550 --> 00:17:57,590
+أسمي الحلقة Alpha ولا Beta بناءً على الـ OH
+
+237
+00:17:57,590 --> 00:18:03,430
+المتصلة بكربونا رقم 1 هل هي للأعلى ولا للأسفل؟
+
+238
+00:18:04,720 --> 00:18:08,600
+بنحكي عن الـ mono-saccharide الـ mono-saccharide
+
+239
+00:18:08,600 --> 00:18:12,700
+حكينا إن هي سكريات وحادية و بتختلف في عدد الراتد
+
+240
+00:18:12,700 --> 00:18:16,760
+الكربون في منها هكسوسوداسية و في منها خماسية بنتوز
+
+241
+00:18:16,760 --> 00:18:20,220
+بيحكيلي في منها كمان السكريات إحنا حكينا بشكل عام
+
+242
+00:18:20,220 --> 00:18:23,740
+في منها عندها تحتوي على aldehyde group في منها
+
+243
+00:18:23,740 --> 00:18:27,540
+تحتوي على كيتونيجون فالـ mono-saccharide بيحكيلي
+
+244
+00:18:27,540 --> 00:18:32,260
+بتنقسم إلى قسمين في منها aldose و في منها ketose
+
+245
+00:18:33,210 --> 00:18:37,510
+ماشي يعني الـ Dose؟ يعني تحتوي على Aldehyde group
+
+246
+00:18:37,510 --> 00:18:41,770
+الـ Dose معناها إنه عندها Aldehyde group طب و
+
+247
+00:18:41,770 --> 00:18:45,030
+حكينا وين الـ Aldehyde group بتكون؟ حكينا بتكون
+
+248
+00:18:45,030 --> 00:18:50,870
+موجودة على كربونا رقم 1 أما Ketose معناها إنه
+
+249
+00:18:50,870 --> 00:18:55,970
+تحتوي على كيتون group و حكينا إن الكيتون بتكون على
+
+250
+00:18:55,970 --> 00:19:00,850
+كربونا رقم 2الذن الـ Dose اللى بتحتوي على الديهيد
+
+251
+00:19:00,850 --> 00:19:05,330
+جروب بتكون على كربونا رقم واحد أشهر مثال على ال ال
+
+252
+00:19:05,330 --> 00:19:09,670
+Dose هو الجلوكوز سكر الجلوكوز يحتوي على الديهيد
+
+253
+00:19:09,670 --> 00:19:15,470
+جروب فبسميه ال Dose و أصلا ال جلوكوز هو سكر هو
+
+254
+00:19:15,470 --> 00:19:20,630
+هكسوز يحتوي على ست ذرات كربو حكينا عنه هكسوز فبقدر
+
+255
+00:19:20,630 --> 00:19:26,470
+أطلق عليه اسم ال Dohexoseالجيلوكوز بقدر أطلق عليه
+
+256
+00:19:26,470 --> 00:19:30,530
+اسم Aldohexose من هون أجت كلمة Aldo لأنه عنده
+
+257
+00:19:30,530 --> 00:19:36,250
+Aldehyde group وhexose لأنه ست ذرات كرم فبالكيتوز
+
+258
+00:19:36,250 --> 00:19:40,270
+اللي تحتوي على كيتون group أشهر مثال عليها هو
+
+259
+00:19:40,270 --> 00:19:46,600
+الفركتوزوالـ Fructose أصلاً هو هكسوز هو يحتوي على
+
+260
+00:19:46,600 --> 00:19:52,300
+6 ذرات كربون اللي هو سداسي فبقدر أطلق عليه اسم
+
+261
+00:19:52,300 --> 00:19:58,280
+كيتوهكسوز كيتوهكسوز معناه أنه يحتوي على كيتون جروب
+
+262
+00:19:58,280 --> 00:20:05,110
+وهو سداسي يحتوي على 6 ذرات كربونبنحكي عن الـ
+
+263
+00:20:05,110 --> 00:20:08,190
+polysaccharide الـ polysaccharide حكينا هي العديد
+
+264
+00:20:08,190 --> 00:20:12,130
+تتسسكر من الأمثلة عليها، عندنا أول مثال الـ
+
+265
+00:20:12,130 --> 00:20:16,190
+amylose الـ amylose هو عبارة عن سلسلة من سكر
+
+266
+00:20:16,190 --> 00:20:20,430
+الجليكوز جليكوز بوليمر، هي الرسمة عندنا الـ
+
+267
+00:20:20,430 --> 00:20:24,690
+structure له للـ amylose أنه عبارة عن سكر الجليكوز
+
+268
+00:20:24,690 --> 00:20:29,620
+متصل مع بعض و لبعض، بوليمر من الجليكوزالفا واحد
+
+269
+00:20:29,620 --> 00:20:34,200
+لاربع لينكش ان هي الرابط الموجودة بينهم اسمها الفا
+
+270
+00:20:34,200 --> 00:20:38,500
+واحد لاربع ليش كيف سميناها الفا واحد لاربع؟ لجيت
+
+271
+00:20:38,500 --> 00:20:41,420
+الفا من وين أجت؟ حكينا من الطلع على كربونا رقم
+
+272
+00:20:41,420 --> 00:20:45,040
+واحد لو جينا على الحلقة الأولى على اليسار تيجي على
+
+273
+00:20:45,040 --> 00:20:49,380
+الكربونا لو طلعت على ال OH هل هي below ولا above؟
+
+274
+00:20:49,380 --> 00:20:55,470
+لاجي ال OH belowالواتش لتحت اسمها Alpha طيب واحد
+
+275
+00:20:55,470 --> 00:20:59,750
+لاربعة هذا اسم الموقع الرابطة بين الجلوكوز الأول
+
+276
+00:20:59,750 --> 00:21:03,370
+والجلوكوز التاني لو جينا على الجلوكوز الأول هيوم
+
+277
+00:21:03,370 --> 00:21:07,970
+رقم اللي على الشمال عندك واحد كربونا رقم واحد تصلت
+
+278
+00:21:07,970 --> 00:21:12,540
+مع كربونا رقم أربعة من الجلوكوز التانيباسم الرابط
+
+279
+00:21:12,540 --> 00:21:18,560
+ألفة واحد لاربعة ألفة عشان الـ OH لتحت واحد لاربعة
+
+280
+00:21:18,560 --> 00:21:22,840
+لأن كربونا رقم واحد من جلوكوزي الأول اتصلت مع
+
+281
+00:21:22,840 --> 00:21:28,090
+كربونا رقم أربعة من جلوكوزي التانيمثال تاني على
+
+282
+00:21:28,090 --> 00:21:31,530
+الـ polysaccharide اللي هو الـ amylobectin الـ
+
+283
+00:21:31,530 --> 00:21:35,850
+amylobectin بيشبه الـ amylose هو عبارة عن مجموعة
+
+284
+00:21:35,850 --> 00:21:39,670
+من الجلوكوز متصل مع بعض و لبعض فهو جلوكوز بوليمر
+
+285
+00:21:39,670 --> 00:21:43,810
+برابط ألف و واحد لأربعة و عرفنا ألفة جاية الـ OH
+
+286
+00:21:43,810 --> 00:21:47,410
+لتحت واحد لأربعة اللي هي كربونا رقم واحد السكر
+
+287
+00:21:47,410 --> 00:21:50,830
+الأول مع كربونا رقم أربعة من السكر التاني ارتبطوا
+
+288
+00:21:50,830 --> 00:21:54,690
+مع بعضبس الزيادة على الـ Amylose إنه عنده branch
+
+289
+00:21:54,690 --> 00:22:00,830
+عنده تفرع من سكر الجلوكوز مكون رابطة اسمها Alpha 1
+
+290
+00:22:00,830 --> 00:22:06,090
+لـ 6 Alpha 1 ل 6 Alpha من وين جاي؟ إنه الـ OH لتحت
+
+291
+00:22:06,090 --> 00:22:12,130
+1 ل 6 إنه التفرع جاي وين؟ عند Carbona رقم 1 من
+
+292
+00:22:12,130 --> 00:22:16,250
+الحلقة اللي فوق مع Carbona رقم 6 من الحلقة اللي
+
+293
+00:22:16,250 --> 00:22:21,180
+تحتإذًا الـ Amylose هو polysaccharide عبارة عن
+
+294
+00:22:21,180 --> 00:22:25,680
+جليكوز بوليمار الرابطة ألف واحد لأربعة Amylopectin
+
+295
+00:22:25,680 --> 00:22:29,920
+برضه هو polysaccharide جليكوز بوليمار عنده ألف
+
+296
+00:22:29,920 --> 00:22:36,700
+لواحد لأربعة وعنده كمان branch رابطة ألف واحد لستة
+
+297
+00:22:40,600 --> 00:22:43,920
+مثال آخر على الـ polysaccharide اللي هي عديد
+
+298
+00:22:43,920 --> 00:22:47,360
+التسكر هو الـ Glycogen الـ Glycogen زي الـ
+
+299
+00:22:47,360 --> 00:22:51,040
+amylobectin عنده رابط عنده polymer من جلكوز ألف
+
+300
+00:22:51,040 --> 00:22:54,880
+واحد لاربعة و عنده branch ألف واحد لستة لكن
+
+301
+00:22:54,880 --> 00:22:58,980
+التفرعات اللي عنده أكتر من تفرعات الـ amylobectin
+
+302
+00:22:58,980 --> 00:23:03,280
+زي الـ I-Glycogen more ألف واحد لستة branch
+
+303
+00:23:03,280 --> 00:23:08,800
+التفرعات زيانةوكل ما زادت التفرعات في السكر، هذا
+
+304
+00:23:08,800 --> 00:23:14,000
+بيجعل السكر ضعيف ويكون سهل تكسّم، فالـ Glycogen
+
+305
+00:23:14,000 --> 00:23:18,160
+عشان تفرعاته أكتر، فهذا بيخليه Rapid glucose
+
+306
+00:23:18,160 --> 00:23:22,620
+release from Glycogen، لما الجسم يحتاج glucose،
+
+307
+00:23:22,620 --> 00:23:26,660
+أسرع يجي أنه يكسّر الـ Glycogen ويعطيني glucose،
+
+308
+00:23:26,660 --> 00:23:31,520
+لأنه عنده تفرعات زيادة، فهذا بيتكسر بسرعة وبيحرر
+
+309
+00:23:31,520 --> 00:23:36,260
+سكر الـ glucose لما يحتاجه الجسم،ليش سهل التكسر
+
+310
+00:23:36,260 --> 00:23:41,980
+بسبب زيادة التفرعات مثال آخر هو الـ cellulose
+
+311
+00:23:41,980 --> 00:23:45,520
+حكينا الـ cellulose يستخدم في الـ plant cell wall
+
+312
+00:23:45,520 --> 00:23:49,800
+في بناء الـ cell wall للـ plant للنبات هو عبارة عن
+
+313
+00:23:49,800 --> 00:23:55,000
+linear chain سلسلة طويلة من السكر ولكن هي الرابطة
+
+314
+00:23:55,000 --> 00:24:01,030
+Beta 1L4بيتا ليش؟ لأن الـ OH للحلقة موجودة above
+
+315
+00:24:01,030 --> 00:24:07,550
+بوجودة للأعلى OH للأعلى، إذا بيكون بيتا 1 ل 4 لأن
+
+316
+00:24:07,550 --> 00:24:11,410
+الرابط بين كل حلقة والتانية جاية من كربون رقم واحد
+
+317
+00:24:11,410 --> 00:24:15,510
+من الحلقة الأولى مع كربون رقم أربع من الحلقة
+
+318
+00:24:15,510 --> 00:24:20,170
+التانية إذا الـ cellulose بيكون نينيار من الـ
+
+319
+00:24:20,170 --> 00:24:27,700
+glucose بوليمر من الـ glucose برابطة بيتا 1 ل 4فيه
+
+320
+00:24:27,700 --> 00:24:31,280
+إنه تقسيم للسكريات بناء على الـ reducing هل هو
+
+321
+00:24:31,280 --> 00:24:35,240
+reducing sugar أو non-reducing sugar في البداية
+
+322
+00:24:35,240 --> 00:24:37,500
+نحكي إيش يعني oxidation وإيش يعني reduction
+
+323
+00:24:37,500 --> 00:24:42,080
+oxidation معناها loss of electron إنه فقد إلكترون
+
+324
+00:24:42,080 --> 00:24:46,080
+بسميه oxidation reduction معناها gain of electron
+
+325
+00:24:46,080 --> 00:24:53,170
+إنه كسب إلكترونالسكر المختزل هو السكر بسميه مختزل
+
+326
+00:24:53,170 --> 00:25:02,030
+السكر
+
+327
+00:25:02,030 --> 00:25:05,870
+المختزل هو السكر اللذي يحتوي على functional group
+
+328
+00:25:05,870 --> 00:25:10,930
+سواء كيتون أو ألدهيد بس تكون free تكون حرة قادرة
+
+329
+00:25:10,930 --> 00:25:16,100
+على المشاركة في تفاعلات الأكسد والاختزادTamam،
+
+330
+00:25:16,100 --> 00:25:19,380
+reducing sugar هو السكر اللي بيحتوي على free
+
+331
+00:25:19,380 --> 00:25:23,860
+aldehyde او free ketone functional group يكون عنده
+
+332
+00:25:23,860 --> 00:25:28,360
+functional group free قادر على المشاركة في تفاعلات
+
+333
+00:25:28,360 --> 00:25:32,480
+الأكسدة والاختزان، لذا الشرط عشان اقول عن هذا
+
+334
+00:25:32,480 --> 00:25:36,700
+السكر ان هو reducing او non-reducingوجود free
+
+335
+00:25:36,700 --> 00:25:41,260
+aldehyde او ketone اجروا الجيت السكر هو reducing
+
+336
+00:25:41,260 --> 00:25:46,620
+sugar هو عامل مختزن يعني هو هيختزل غيره وهو
+
+337
+00:25:46,620 --> 00:25:52,770
+هيصيرله أكسداreducing sugar يعني سكر مختزل مختزل
+
+338
+00:25:52,770 --> 00:25:57,150
+يعني هو بده يختزل غيره بده يعمل اختزال لغيره و هو
+
+339
+00:25:57,150 --> 00:26:01,470
+نفسه بده يصيرله أكسده مين بده يأكسده؟ أكيد عامل
+
+340
+00:26:01,470 --> 00:26:06,270
+مؤكسد بده يجي أكسد هذا السكر و هو يصيرله أختزال
+
+341
+00:26:06,270 --> 00:26:10,010
+فعشان مكتوب sugar can be oxidized by mild
+
+342
+00:26:10,010 --> 00:26:16,850
+oxidizing agent السكر بيتأكسد بوجود عامل مؤكسد
+
+343
+00:26:17,410 --> 00:26:22,390
+والعمل المؤكسد هذا and oxidizing agent is reduced
+
+344
+00:26:22,390 --> 00:26:25,770
+in the reaction هيصير له اختزاة، اذا العامل
+
+345
+00:26:25,770 --> 00:26:31,050
+المختزل اللي هو السكر هذا بيختزل غيره، هو بيصير له
+
+346
+00:26:31,050 --> 00:26:37,150
+أكسدة، العامل المؤكسد بيأكسد غيره، هو بيصير له
+
+347
+00:26:37,150 --> 00:26:43,200
+اختزاةأجيت لما يتأكسد السكر هيتأكسد إلى acid
+
+348
+00:26:43,200 --> 00:26:47,220
+ويعطيني مركب تاني من السكريات اللي هي reducing
+
+349
+00:26:47,220 --> 00:26:50,700
+sugar بيحكي ل reducing sugar هم كل ال mono
+
+350
+00:26:50,700 --> 00:26:55,400
+-saccharide كل السكريات الأحدى دي هي عبارة عن
+
+351
+00:26:55,400 --> 00:26:59,950
+reducing sugar و ال maltose و ال lactoseكل
+
+352
+00:26:59,950 --> 00:27:04,990
+السكريات الأحادية وبالإضافة عليهم الـ maltose والـ
+
+353
+00:27:04,990 --> 00:27:08,430
+lactose من الـ disaccharide كل الـ monosaccharide
+
+354
+00:27:08,430 --> 00:27:10,950
+وعندنا الـ maltose والـ lactose من الـ
+
+355
+00:27:10,950 --> 00:27:17,420
+disaccharide هدولة بس هم يعتبروا reducing sugarزي
+
+356
+00:27:17,420 --> 00:27:20,620
+ما حكينا، مين هم الـ Oxidized اللي هم الـ Reducing
+
+357
+00:27:20,620 --> 00:27:26,080
+Sugar اللي بيقدر يصيرلهم أكسدة و هم يختزلوا غيرهم
+
+358
+00:27:26,080 --> 00:27:30,400
+كل الـ Monosaccharide و الـ Disaccharide بنعتبرهم
+
+359
+00:27:30,400 --> 00:27:35,580
+Reducing Sugar، بيصيرلهم أكسدة، لأن السكريات بتكون
+
+360
+00:27:35,580 --> 00:27:39,740
+موجودة في المحلول، بتكون على شكل خلية متوازن من
+
+361
+00:27:39,740 --> 00:27:43,940
+open chain و closed ring في منها بتكون open مفتوحة
+
+362
+00:27:43,940 --> 00:27:48,190
+خطية و في منها بتكون حلقيةكل الـ Monosaccharide
+
+363
+00:27:48,190 --> 00:27:52,150
+بتكون open و closed لكن الـ Oligosaccharide بس فيه
+
+364
+00:27:52,150 --> 00:27:55,890
+منه closed structure، only closed when cyclic
+
+365
+00:27:55,890 --> 00:27:58,690
+structure open، the aldehyde group is available
+
+366
+00:27:58,690 --> 00:28:02,290
+for oxidation مدى ما تكون عندي حلقة و أعملها open،
+
+367
+00:28:02,290 --> 00:28:05,890
+أفتحها وتصير خطية، بتكون ال functional group فيها
+
+368
+00:28:05,890 --> 00:28:10,770
+تقدر تدخل في تفاعلات الأكسدة والاختزال، يعني بتصير
+
+369
+00:28:10,770 --> 00:28:11,470
+free
+
+370
+00:28:14,480 --> 00:28:18,080
+حينها الشكل للـ Open و لـ Closed Structure of
+
+371
+00:28:18,080 --> 00:28:21,340
+Monosaccharide كيف بيكون شكلها على اليسار عندنا
+
+372
+00:28:21,340 --> 00:28:25,120
+شكل الـ Open كيف شكل الـ Linear لـ الـ Structure
+
+373
+00:28:25,120 --> 00:28:31,060
+Linear لـ السكريات في ال .. اللي على اليمين عندنا
+
+374
+00:28:31,060 --> 00:28:33,920
+الشكل الحلقى الشكل المغلق بيحكي اللي في الشكل
+
+375
+00:28:33,920 --> 00:28:38,140
+الحلقى بتكون ال .. ال functional group بتكون شفري
+
+376
+00:28:38,140 --> 00:28:42,880
+لما أفتحه أنا بيصير free functional groupبتقدر
+
+377
+00:28:42,880 --> 00:28:49,300
+تشارك في تفاعلات الأكسدا والاختزاة هنا سؤال، سؤال
+
+378
+00:28:49,300 --> 00:28:52,220
+بيحكي الـ Lactose is reducing sugar why؟ ليش الـ
+
+379
+00:28:52,220 --> 00:28:55,440
+Lactose هو يعتبر reducing sugar؟ زي ما احنا عارفين
+
+380
+00:28:55,440 --> 00:28:59,100
+ان الـ Lactose هو عبارة عن سكر ثونائي اتكون من
+
+381
+00:28:59,100 --> 00:29:04,000
+اتحاد سكر، الـ glucose و الـ galactose مع بعض،
+
+382
+00:29:04,000 --> 00:29:08,100
+برابط السكريين أجيت هدول السكريين لما يتحدوا هما
+
+383
+00:29:08,100 --> 00:29:11,020
+الاتنين، كل واحد عنده functional group من هدول
+
+384
+00:29:11,020 --> 00:29:15,630
+السكريينتحكيلي في الرابط السكرية بتيجي بتتكون في
+
+385
+00:29:15,630 --> 00:29:20,170
+هدول السكريين بين functional group من السكر الأول
+
+386
+00:29:20,170 --> 00:29:24,190
+مع موقع أخر في السكر التاني يعني في واحد من
+
+387
+00:29:24,190 --> 00:29:27,470
+السكريات بتكون الـfunctional group له مشغولة في
+
+388
+00:29:27,470 --> 00:29:31,250
+تكوين الرابط السكرية لكن السكر التاني بتكون
+
+389
+00:29:31,250 --> 00:29:34,660
+الـfunctional group عنده حرةلو جيتوا و طلعتوا على
+
+390
+00:29:34,660 --> 00:29:37,500
+تحت الـ Structure اللي تحتها ده، في عندنا رسم
+
+391
+00:29:37,500 --> 00:29:40,740
+تاني، حلق تاني، واحدة موفمان هم الرابط، الرابط
+
+392
+00:29:40,740 --> 00:29:44,000
+السكرية اللي هي رابطة التنمع بقى، لو جينا شوفنا
+
+393
+00:29:44,000 --> 00:29:47,480
+الـ Carbonyl group للحلقة الأولى اللي علي يسار،
+
+394
+00:29:47,480 --> 00:29:51,180
+عند رقم واحد هاي، هاي ال functional group، مشغولة
+
+395
+00:29:51,180 --> 00:29:54,340
+هنا، مشغولة في واش، في تكوين الرابطة مرتبطة مع
+
+396
+00:29:54,340 --> 00:29:57,770
+الحلقة التانيةلكن لو جينا للحلقة اللي عاليمين و
+
+397
+00:29:57,770 --> 00:30:00,790
+للـ Carbonyl group اللي هي الـ Carbonyl group هي
+
+398
+00:30:00,790 --> 00:30:03,790
+نفسها الـ C double bond O هي الـ functional group
+
+399
+00:30:03,790 --> 00:30:07,770
+موجودة على أقصى اليمين مش مشتركة حرة free على
+
+400
+00:30:07,770 --> 00:30:12,090
+الجنب مش مشتركة في تكوين الرفضةوالشرط عشان السكر
+
+401
+00:30:12,090 --> 00:30:17,410
+يكون reducing sugar هو وجود free carbonyl group أو
+
+402
+00:30:17,410 --> 00:30:21,650
+free functional group وهي موجودة هنا في هذا السكر
+
+403
+00:30:21,650 --> 00:30:25,010
+في اللكتوز إذا واحد من السكريات عنده free
+
+404
+00:30:25,010 --> 00:30:29,710
+functional group عشان هيك هو يعتبر reducing sugar
+
+405
+00:30:29,710 --> 00:30:33,290
+لأنه عنده free carbonyl group صحيح التاني مشغولة
+
+406
+00:30:33,290 --> 00:30:37,830
+بس المهم في واحدة free بتشارك في تفاعلات الأكسدة
+
+407
+00:30:37,830 --> 00:30:42,870
+والاختزانمن عندنا الـ non-reducing sugar؟ non
+
+408
+00:30:42,870 --> 00:30:46,230
+-reducing sugar معناها إنه not oxidized by mild
+
+409
+00:30:46,230 --> 00:30:51,150
+oxidizing agent يعني مش هيصيرلهم أكسدة بوجود عامل
+
+410
+00:30:51,150 --> 00:30:55,030
+مؤكسد، لا هم هيختزلوا غيرهم ولا هم يصيرلهم أكسدة،
+
+411
+00:30:55,030 --> 00:31:00,110
+من هم؟ هم الساكروز وكل ال polysaccharides الساكروز
+
+412
+00:31:00,110 --> 00:31:04,370
+وكل ال polysaccharides يعتبروا non-reducing sugar
+
+413
+00:31:05,600 --> 00:31:09,620
+طيب الساكروز is not reducing sugar, why؟ ليش
+
+414
+00:31:09,620 --> 00:31:13,900
+الساكروز مش reducing sugar؟ بيحكيلي الساكروز هو
+
+415
+00:31:13,900 --> 00:31:19,700
+سكر ثونائي عبارة عن اتحاد سكر الجولكوز والفراكتوز
+
+416
+00:31:19,700 --> 00:31:24,020
+اتحاد سكر الجولكوز بالفراكتوزبحكي لما ييجوا هدول
+
+417
+00:31:24,020 --> 00:31:27,560
+السكريين يتحدى عشان يكونوا للـSaccharose بيرتبطوا
+
+418
+00:31:27,560 --> 00:31:30,480
+مع بعض وبتيجى الرابط السكرية بينهم بين
+
+419
+00:31:30,480 --> 00:31:33,740
+الـfunctional group للسكر الأول والـfunctional
+
+420
+00:31:33,740 --> 00:31:37,220
+group للسكر التاني، اذا الرابط السكرية ده بتيجى
+
+421
+00:31:37,220 --> 00:31:40,680
+بين الـfunctional group للسكر الأول والـfunctional
+
+422
+00:31:40,680 --> 00:31:44,140
+group للسكر التاني، يعني الكربونيلي group من السكر
+
+423
+00:31:44,140 --> 00:31:48,480
+الأول بتيجى مع الكربونيلي group من السكر التانيفما
+
+424
+00:31:48,480 --> 00:31:51,000
+انهم مرتبطوا من عند الـ functional group في
+
+425
+00:31:51,000 --> 00:31:55,380
+الحالتين يبقى فيش free functional group وبالتالي
+
+426
+00:31:55,380 --> 00:31:59,300
+بتكون الـ carbonyl group are busy in the two sides
+
+427
+00:31:59,300 --> 00:32:04,200
+بتكون مشغولة في الجهتين ففيش free functional group
+
+428
+00:32:04,470 --> 00:32:09,410
+تشارك في تفاعلات الأكسد والاختزاق فالسكراوز not
+
+429
+00:32:09,410 --> 00:32:13,010
+reducing sugar لأنه اختل شرط أن يكون reducing
+
+430
+00:32:13,010 --> 00:32:17,190
+sugar وهو وجود free functional group مافيش عنده
+
+431
+00:32:17,190 --> 00:32:20,170
+free functional group، they are busy in the two
+
+432
+00:32:20,170 --> 00:32:24,410
+sides، في السكريين مشغولة في تكوين الرابط السكرية
+
+433
+00:32:26,160 --> 00:32:30,680
+إيش هي ال oxidizing agent اللي ممكن استخدمها لكشف
+
+434
+00:32:30,680 --> 00:32:35,920
+عن وجود reducing sugar لازم يكون عنده سكر بيختزل
+
+435
+00:32:35,920 --> 00:32:39,520
+غيره و عامل مواكسيد عشان هو ي access ال sugar مين
+
+436
+00:32:39,520 --> 00:32:42,300
+هم العوامل الواكسيدة اللي ممكن استخدمها؟ هي
+
+437
+00:32:42,300 --> 00:32:47,780
+البنديكسولوشن، فيه فيلنكسولوشن، طولنزرياجن و بيكرك
+
+438
+00:32:47,780 --> 00:32:53,260
+أسدسولوشنBendects, Filling, Tolens, Bacaric Acid
+
+439
+00:32:53,260 --> 00:32:57,480
+إيش هو الـ Oxidizing Agent؟ الـ Oxidizing Agent هو
+
+440
+00:32:57,480 --> 00:33:01,680
+العامل اللي اسمه تاني Oxidizer or Oxidant العامل
+
+441
+00:33:01,680 --> 00:33:06,380
+اللي بيأكسد غيره هو نفسه بيختزن تعريفه Chemical
+
+442
+00:33:06,380 --> 00:33:10,920
+Compound That Transfers Oxygen Atom هو مركب كيميا
+
+443
+00:33:10,920 --> 00:33:16,740
+ينقل ضرر أكسجين أو هو اللي بيكسب إلكترون إلكترون
+
+444
+00:33:16,740 --> 00:33:21,710
+in Redox chemical reactionهدأ الـ reduction طيب
+
+445
+00:33:21,710 --> 00:33:24,490
+الألدوز اللي جيت احنا لما يصير عندنا oxidation
+
+446
+00:33:24,490 --> 00:33:28,030
+reduction reaction السكر reducing sugar ليش هيتحول
+
+447
+00:33:28,030 --> 00:33:31,610
+لما يصير له oxidation؟ لأن السكر هو عامل مختزل،
+
+448
+00:33:31,610 --> 00:33:35,790
+بدي يصير له أكسدة، فالسكر هيختزل إلى أيه؟ هيتأكسد
+
+449
+00:33:35,790 --> 00:33:39,490
+إلى أيش؟ إذا كان السكر نوع ألدوز عنده aldehyde
+
+450
+00:33:39,490 --> 00:33:42,830
+group زي الجوليكوز، لما يصير له oxidation، بتحول
+
+451
+00:33:42,830 --> 00:33:47,770
+إلى حاجة اسمها carboxylateأما إذا كان السكر نوعه
+
+452
+00:33:47,770 --> 00:33:52,190
+كيتوز عنده كيتوني جروب زي الفركتوز مايصير
+
+453
+00:33:52,190 --> 00:33:56,810
+oxidation بتحول لحاجة اسمها hydroxylcarboxylate
+
diff --git a/PL9fwy3NUQKwZ1Q7zOg1ObgufPMjRI3WY1/OOPghZ3VTGI.srt b/PL9fwy3NUQKwZ1Q7zOg1ObgufPMjRI3WY1/OOPghZ3VTGI.srt
new file mode 100644
index 0000000000000000000000000000000000000000..13826fe3d42487776eea92345321fc16708f4e72
--- /dev/null
+++ b/PL9fwy3NUQKwZ1Q7zOg1ObgufPMjRI3WY1/OOPghZ3VTGI.srt
@@ -0,0 +1,707 @@
+1
+00:00:00,000 --> 00:00:04,560
+الـ Solution هو جهاز لفصل المواد حسب الكثافة
+
+2
+00:00:04,560 --> 00:00:10,780
+الثقيل ينزل تحت والخفيف يكون في الأعلى جهاز لفصل
+
+3
+00:00:10,780 --> 00:00:14,580
+المواد بناء على الكثافة Separate materials of
+
+4
+00:00:14,580 --> 00:00:19,000
+varying density تستيتيوط قنابيب نستخدمها عشان
+
+5
+00:00:19,000 --> 00:00:22,800
+نشتغل فيها الفحوصات نحط فيها small amount of
+
+6
+00:00:22,800 --> 00:00:28,880
+solution قنابيب تحتوي على أو تتسع إلى كميات قليلة
+
+7
+00:00:28,880 --> 00:00:33,930
+من المحلول Thermometer بجهاز فيه الحرارة measure
+
+8
+00:00:33,930 --> 00:00:39,670
+temperature وير جوز هي شبكة موزعة للحرارة Wire gauze
+
+9
+00:00:39,670 --> 00:00:43,670
+شبكة موزعة للحرارة بحطها فوق اللهب بتعمل spread
+
+10
+00:00:43,670 --> 00:00:50,430
+heat بتوزع الحرارة اللي بيوريت هي الصحاحة كده
+
+11
+00:00:50,430 --> 00:00:54,430
+استخدمتوها في الكيميا اللي بنستخدمها في عمليات ال
+
+12
+00:00:54,430 --> 00:00:59,750
+titration نحط فيها جواه سائل بنسميه titrant الـ
+
+13
+00:00:59,750 --> 00:01:04,330
+بيوريت صحاحتها تستخدم للمعايرة للـ titration تحتوي
+
+14
+00:01:04,330 --> 00:01:08,370
+على السائل في داخلها بنسميه titrant كيف بدي أقرأ
+
+15
+00:01:08,370 --> 00:01:13,550
+منها major volume نقرأ القراءة من سطح التقعر من
+
+16
+00:01:13,550 --> 00:01:19,220
+سطح التقعر كتم القراءة هنا حاجة بنتسميها certainty
+
+17
+00:01:19,220 --> 00:01:22,700
+و حاجة بنتسميها uncertainty اللي هي القيمة اللي
+
+18
+00:01:22,700 --> 00:01:26,820
+أنا متأكدة منها طويق مشاك فيها هو أنا مثلا في
+
+19
+00:01:26,820 --> 00:01:30,260
+البيوت في الصحاحة بدي أجي أقرأ ال volume حكي أنا
+
+20
+00:01:30,260 --> 00:01:33,560
+بنقرأ من سطح التقعر لو جينا شوفنا الصورة اللي على
+
+21
+00:01:33,560 --> 00:01:38,000
+الجنب في ال slide هلجيت القراءة جاي فوق ال 36
+
+22
+00:01:38,000 --> 00:01:43,750
+بشوية تمام؟ هي هي 35 الشرطة اللي فوقها 36 هي بعيدها
+
+23
+00:01:43,750 --> 00:01:48,510
+بشوية يعني القيمة المقروءة هتكون 36 وإشي ممكن
+
+24
+00:01:48,510 --> 00:01:51,810
+واحدة تيجي تقولي إنها 36 ونصف واحدة تانية تقولي
+
+25
+00:01:51,810 --> 00:01:56,770
+36 و 6 واحدة تقولي 36 و 4 لكن القيمة اللي أنا
+
+26
+00:01:56,770 --> 00:02:01,320
+متأكدة منها ال certainty إنها 36 والقيمة اللي فيها
+
+27
+00:02:01,320 --> 00:02:05,040
+الشك اللي هيتختلف من one ل two اللي هي الأعشار
+
+28
+00:02:05,040 --> 00:02:08,320
+اللي جنبها اللي هي الخمسة من عشرة ستة من عشرة
+
+29
+00:02:08,320 --> 00:02:14,400
+أربعة من عشرة فالفرق بيكون زائد أو ناقص واحد من
+
+30
+00:02:14,400 --> 00:02:18,720
+عشرة هاي بتكون القيمة ال uncertainty تمام
+
+31
+00:02:18,720 --> 00:02:21,400
+certainty اللي أنا متأكدة منها في الصورة مثلا
+
+32
+00:02:21,400 --> 00:02:26,000
+للقراءة هاي أنها 36 Uncertainty هي الأعشار اللي
+
+33
+00:02:26,000 --> 00:02:29,100
+جنبها، اللي هو أنا .. هي مش على ال 36 بالظبط، هي
+
+34
+00:02:29,100 --> 00:02:33,000
+فوقها في شوية، يعني ممكن تكون 36 ونص، ممكن 36 و
+
+35
+00:02:33,000 --> 00:02:38,350
+6، ممكن 36 و أعمل الـ Certainty بتعتمد على حاجة
+
+36
+00:02:38,350 --> 00:02:42,370
+بنسميها precision على الدقة الـ Certainty و الـ
+
+37
+00:02:42,370 --> 00:02:45,890
+Measurement depends on the precision بتعتمد على
+
+38
+00:02:45,890 --> 00:02:50,010
+الدقة يعني الدقة مثلا في الأدوات اللي مقسمة أكثر
+
+39
+00:02:50,010 --> 00:02:54,410
+هتكون الدقة أكتر فيها مجسمة لا عشار ولا مجسمة بس
+
+40
+00:02:54,410 --> 00:02:58,650
+لعداد صحيحة مين هتكون أدق؟ أكيد اللي مجسمة لا عشار
+
+41
+00:02:58,650 --> 00:02:59,710
+هي الأدق
+
+42
+00:03:02,280 --> 00:03:06,680
+هنا مصطلحين مهمين اللي هم بتسميهم accuracy و
+
+43
+00:03:06,680 --> 00:03:11,220
+precision نيجي نتعرف على accuracy ماذا تعني؟
+
+44
+00:03:11,220 --> 00:03:16,000
+accuracy تعني مدى قرب النتيجة من النتيجة الحقيقية
+
+45
+00:03:16,000 --> 00:03:20,900
+درجة الانتباه من الحقيقة ال degree of conformity
+
+46
+00:03:20,900 --> 00:03:26,080
+to the truth and experiment مدى قرب النتيجة من
+
+47
+00:03:26,080 --> 00:03:31,950
+النتيجة الحقيقية كيف يعني؟ يعني أنا بحكيلك أن فيها
+
+48
+00:03:31,950 --> 00:03:36,290
+نفاح سكر اشتغلت وطلعت مية خذ اشتغليها وشوفيها
+
+49
+00:03:36,290 --> 00:03:39,710
+قيمتها الحقيقية أنا حكيتلك أنَّها مية وأنت اشتغلتيها
+
+50
+00:03:39,710 --> 00:03:44,070
+و طلعت معاكي سبعين هل السبعين قريبة من المية؟ لا
+
+51
+00:03:44,070 --> 00:03:47,890
+فهي بنسميها inaccurate result أنت ما طلعتيهاش
+
+52
+00:03:47,890 --> 00:03:51,750
+accurate لكن لو طلعت معاكي تسعة وتسعين تسعة و
+
+53
+00:03:51,750 --> 00:03:55,770
+تسعين قريبة من المية إذن accurate result accuracy
+
+54
+00:03:55,770 --> 00:04:00,820
+مدى قرب النتيجة من النتيجة الحقيقية فبالـ Precision
+
+55
+00:04:00,820 --> 00:04:05,080
+الـ Precision هي مدى قرب النتائج من بعضها البعض
+
+56
+00:04:05,080 --> 00:04:09,380
+degree of agreement between replicated experiments
+
+57
+00:04:12,600 --> 00:04:17,340
+و طلعت النتائج في كل مرة يعني نفس الفحص اشتغلته خمس
+
+58
+00:04:17,340 --> 00:04:21,900
+مرات تمام؟ طلعت معايا القيمة الأولى 72 القيمة
+
+59
+00:04:21,900 --> 00:04:28,500
+الثانية 74 القيمة الثالثة 71 القيمة الرابعة 73 هل
+
+60
+00:04:28,500 --> 00:04:31,980
+هم قريبين من بعض؟ هم كلهم قريبين من بعض فهي
+
+61
+00:04:31,980 --> 00:04:37,180
+precise result نتائجك كانت precise Precision مدى
+
+62
+00:04:37,180 --> 00:04:40,760
+قربها من بعضها البعض إن أنا أعيد الفحص أكثر من مرة
+
+63
+00:04:40,760 --> 00:04:44,560
+و أشوف النتائج هل قريبة من بعض ولا لا Accuracy قرب
+
+64
+00:04:44,560 --> 00:04:47,500
+النتيجة من النتيجة الحقيقية أنا بكون معطياكي
+
+65
+00:04:47,500 --> 00:04:51,100
+النتيجة وأنت اشتغليها هل طلعت نتيجتي مقاربة
+
+66
+00:04:51,100 --> 00:04:55,280
+لنتيجتي فهنا بتكون accurate result إذا طلعت بعيد
+
+67
+00:04:55,280 --> 00:05:00,780
+عنها بتكون not accurate، inaccurate result طبعًا؟
+
+68
+00:05:00,780 --> 00:05:05,000
+طب أنا حكيتلك إنه عندي أنا فحص اشتغلت وطلعت 100
+
+69
+00:05:05,760 --> 00:05:09,160
+وأنت اشتغلي ليها أكثر من مرة وشوفي ليها accurate
+
+70
+00:05:09,160 --> 00:05:13,460
+or precise اشتغلتي أنا وحكيتلك أنَّها 100، أنتِ
+
+71
+00:05:13,460 --> 00:05:19,020
+اشتغلتيها خمس مرات، طلعت معاكي مرة 101، مرة 99، مرة
+
+72
+00:05:19,020 --> 00:05:24,380
+102، مرة 98، هلجيت النتائج قريبة من بعض، كانت 101،
+
+73
+00:05:24,380 --> 00:05:31,000
+99، 102، 98 ما أنها قريبة من بعض هتكون precise طب
+
+74
+00:05:31,000 --> 00:05:34,520
+النتيجة الحقيقية كانت مية وكانوا قراب على المية
+
+75
+00:05:34,520 --> 00:05:38,700
+كلهم، لذا هي precise و accurate جمعت من الإثنين
+
+76
+00:05:38,700 --> 00:05:42,080
+كانت precise و accurate مع بعض
+
+77
+00:05:45,090 --> 00:05:48,870
+نحكي عن الـ chemical reaction التفاعل الكيميائي
+
+78
+00:05:48,870 --> 00:05:54,390
+التفاعل الكيميائي هو تفاعل تدخل فيه مادة تطلع عنها
+
+79
+00:05:54,390 --> 00:06:00,210
+مادة جديدة بصفات جديدة تغيير مادتك إلى مادة جديدة
+
+80
+00:06:00,210 --> 00:06:03,310
+بخصائص
+
+81
+00:06:03,310 --> 00:06:08,410
+مختلفة تدخل مادة جديدة بخصائص مختلفة Sign of
+
+82
+00:06:08,410 --> 00:06:13,110
+chemical reaction علامات حدوث التفاعل أول شيء ممكن
+
+83
+00:06:13,110 --> 00:06:16,950
+يتكون عندي راسب هذا يدل على حدوث التفاعل Formation
+
+84
+00:06:16,950 --> 00:06:20,910
+of precipitate Color change ممكن يتغير اللون
+
+85
+00:06:20,910 --> 00:06:26,070
+Evolution of gas يعني تصاعد غاز تصاعد غاز يعني
+
+86
+00:06:26,070 --> 00:06:30,130
+حدوث عندي تغير Temperature change تتغير درجة
+
+87
+00:06:30,130 --> 00:06:35,120
+الحرارة برضه هو علامة من علامات حدوث التفاعل كيف
+
+88
+00:06:35,120 --> 00:06:38,720
+تتغير درجة الحرارة؟ في عندنا نوعين من التفاعل في
+
+89
+00:06:38,720 --> 00:06:44,020
+تفاعل اسمه Exothermic Reaction يعني طارد للحرارة
+
+90
+00:06:44,020 --> 00:06:48,640
+يعني بيحتاجش الحرارة، بيطلع الحرارة لبرا فبتكون
+
+91
+00:06:48,640 --> 00:06:52,280
+الأنبوبة لما تحسيها من برا بتكون ساخنة to get
+
+92
+00:06:52,280 --> 00:06:56,140
+hotter surrounding to get hot أوتة يكون الخارج
+
+93
+00:06:56,140 --> 00:07:00,520
+المحيط الخارجي ساخن لأنه طارد للحرارة endothermic
+
+94
+00:07:00,520 --> 00:07:05,860
+reaction ماصل الحرارة تفاعل ماصل الحرارة تحتاج
+
+95
+00:07:05,860 --> 00:07:10,560
+لحرارة بتاخد حرارة فبيكون المحيط الخارجي بارد cool
+
+96
+00:07:13,910 --> 00:07:17,050
+عشان معاها ال biochemical compound اللي هنتعرف
+
+97
+00:07:17,050 --> 00:07:21,190
+عليها هنتعرف على ال carbohydrates على ال amino
+
+98
+00:07:21,190 --> 00:07:25,130
+acid و ال protein هنتعرف على ال nucleic
+
+99
+00:07:25,130 --> 00:07:31,180
+types والنيوكليك أسيد هتاخدوها في مادة ثانية بدنا
+
+100
+00:07:31,180 --> 00:07:35,400
+اليه ندخل في مقدمة بسيطة عن الـ amino acid الأحماض
+
+101
+00:07:35,400 --> 00:07:39,220
+الأمينية وإن شاء الله المرة اللي جاي هنكملها هذا
+
+102
+00:07:39,220 --> 00:07:44,440
+الموضوع الأحماض الأمينية الـ amino acid إيش هي؟ هي
+
+103
+00:07:44,440 --> 00:07:48,880
+الـ building blocks of البروتين هي الوحدة البنائية
+
+104
+00:07:48,880 --> 00:07:53,760
+لتركيب البروتين تكون مرتبطة مع بعضها linked to
+
+105
+00:07:53,760 --> 00:07:58,240
+peptide bond أحماض أمينية مجموعة أحماض أمينية بتكون
+
+106
+00:07:58,240 --> 00:08:02,420
+مرتبطة مع بعض في peptide bond في رابطة ببتيدية
+
+107
+00:08:02,420 --> 00:08:06,680
+لما يرتبطوا مع بعض هدول بيكونوا لي البروتين فالحمض
+
+108
+00:08:06,680 --> 00:08:12,180
+الأميني هو الوحدة البنائية لتركيب البروتين وترتبط
+
+109
+00:08:12,180 --> 00:08:17,930
+مع بعضها بروابط ببتيدية بـ peptide bond إيش تركيب
+
+110
+00:08:17,930 --> 00:08:21,690
+هذا الحمض الأمين؟ لو نطلع على الصورة اللي عندنا
+
+111
+00:08:21,690 --> 00:08:26,530
+كيف تركيبها بتكون بالنص في عندنا ذرة Carbon هذه
+
+112
+00:08:26,530 --> 00:08:30,090
+الـ Carbon بنسميها Central Carbon اللي هي الكربون
+
+113
+00:08:30,090 --> 00:08:34,970
+المركزية أو إلها اسم ثاني Alpha Carbon أو اسمها
+
+114
+00:08:34,970 --> 00:08:38,900
+الثالث Primary Carbon هي عندنا في الوسط في تركيب
+
+115
+00:08:38,900 --> 00:08:41,660
+الحمض الأميني بتكون عندنا الـ Central Carbon
+
+116
+00:08:41,660 --> 00:08:46,960
+كربونة مركزية أو إيه تسميها ثاني Alpha Carbon أو
+
+117
+00:08:46,960 --> 00:08:50,900
+Primary Carbon هاي الـ Central Carbon بتكون مرتبطة
+
+118
+00:08:50,900 --> 00:08:56,080
+بأربع روابط بتكون مرتبطة بجهة بمجموعة بنسميها
+
+119
+00:08:56,080 --> 00:09:01,950
+Carboxyl Group Carboxyl Group اللي هي COOH الـ
+
+120
+00:09:01,950 --> 00:09:06,050
+Carboxyl group و بتكون من جهة الثانية مرتبطة
+
+121
+00:09:06,050 --> 00:09:11,050
+بمجموعة ... بذرة هيدروجين بـ H و من جهة بتكون
+
+122
+00:09:11,050 --> 00:09:15,330
+مرتبطة بـ Amino group لأن NH3 plus بنسميها
+
+123
+00:09:15,330 --> 00:09:20,150
+Amino group و من الأسفل بتكون مرتبطة بالـ R group
+
+124
+00:09:20,150 --> 00:09:24,890
+الـ R group هاي اسمها الـ side chain الـ side
+
+125
+00:09:24,890 --> 00:09:28,970
+chain هذه هي اللي بتميز الحمض الأميني عن غيره هي
+
+126
+00:09:28,970 --> 00:09:32,330
+عبارة عن مجموعة بتكون مرتبطة ممكن تكون ذرة واحدة
+
+127
+00:09:32,330 --> 00:09:37,250
+وممكن تكون مجموعة هي اللي بتميز كل حمض أميني عن
+
+128
+00:09:37,250 --> 00:09:41,290
+الآخر كل الأحماض الأمينية لها نفس التركيب بتكون
+
+129
+00:09:41,290 --> 00:09:45,730
+ذرة كربون و هيدروجين و كربوكسيل و أمينو و الـ R
+
+130
+00:09:45,730 --> 00:09:49,590
+group هذي هي اللي بتختلف من حمض أميني للآخر يعني
+
+131
+00:09:49,590 --> 00:09:54,570
+اللي بيميز حمض أميني عن الآخر هي الـ R group تمام؟
+
+132
+00:09:55,130 --> 00:09:59,930
+وكل الأحماض الأمينية عندها amino group أو primary
+
+133
+00:09:59,930 --> 00:10:03,650
+amino group اللي هي مجموعة الأمين العضوية بنسميها
+
+134
+00:10:03,650 --> 00:10:10,130
+amino group أو primary amino group ماعدا حمض أمين
+
+135
+00:10:10,130 --> 00:10:14,750
+واحد اسمه البرولين البرولين ماعندهوش amino عنده
+
+136
+00:10:14,750 --> 00:10:19,830
+حاجة بنسميها secondary amino group
+
+137
+00:10:21,080 --> 00:10:26,960
+كلهم عندهم amino group ماعدا البرولين عنده amino
+
+138
+00:10:26,960 --> 00:10:31,480
+group أو secondary amino group ليش سموها هكذا؟
+
+139
+00:10:31,480 --> 00:10:34,920
+عشان مجموعة ال amino هاي اللي على الجنب الـ NH3
+
+140
+00:10:34,920 --> 00:10:38,880
+plus بتكون مرتبطة مع الـ R و مع الـ C عاملة حلقة
+
+141
+00:10:38,880 --> 00:10:44,210
+بينها وبين نفسها بتكون الـ Amino Group مرتبطة مع
+
+142
+00:10:44,210 --> 00:10:47,550
+الـ R من جهة مع الـ Side Chain ومع الكربون
+
+143
+00:10:47,550 --> 00:10:50,910
+المركزية من جهة، ما تكونش مرتبطة برابطة واحدة مع
+
+144
+00:10:50,910 --> 00:10:54,090
+الكربون زي الصورة وخلاص، لا، بتكون مرتبطة من
+
+145
+00:10:54,090 --> 00:10:58,390
+جهتها، جهة مع الكربون وجهة مع الـ R، مع الـ Side
+
+146
+00:10:58,390 --> 00:11:03,030
+Chain، هنا ما ضلتش اسمها Primary Amino Group زي أي
+
+147
+00:11:03,030 --> 00:11:06,410
+حمض أميني مرتبطة بجهة واحدة، سموها Primary، زي
+
+148
+00:11:06,410 --> 00:11:10,910
+مرتبطة في الكربونة وخلاص إذا مرتبطة مع الكربونة ومع
+
+149
+00:11:10,910 --> 00:11:15,690
+الـ R زي في حالة البرولين وهو الحالة الوحيدة الحمض
+
+150
+00:11:15,690 --> 00:11:21,790
+الوحيد بنسميها secondary amino group 
+
+151
+00:11:21,790 --> 00:11:28,130
+group في عندنا الـ food are divided into three
+
+152
+00:11:28,130 --> 00:11:31,510
+classes طعام مقسم إلى ثلاثة في عندنا carbohydrate
+
+153
+00:11:31,510 --> 00:11:35,310
+و lipid و protein طعام بتكون من ثلاثة
+
+154
+00:11:35,310 --> 00:11:39,270
+carbohydrate، lipid و protein ال carbohydrate
+
+155
+00:11:39,270 --> 00:11:43,770
+تعتبر هي مصدر الطاقة source of energy ال lipid
+
+156
+00:11:43,770 --> 00:11:49,250
+تعتبر مخزن الطاقة energy reserve ال protein هي
+
+157
+00:11:49,250 --> 00:11:53,830
+energy for growth and cellular maintenance هي عبارة
+
+158
+00:11:53,830 --> 00:11:59,190
+عن طاقة الخلايا المستخدمة للنمو لبناء الجسم إذا
+
+159
+00:11:59,190 --> 00:12:03,410
+عندنا ثلاثة نجد إحنا حكينا إنه البروتين كيف بتكون
+
+160
+00:12:03,410 --> 00:12:07,550
+بتيجي مجموعة أحماض أمينية ترتبط مع بعضها البعض
+
+161
+00:12:07,550 --> 00:12:11,450
+بيترتبط high point في روابط ببتيدية بتكوين
+
+162
+00:12:11,450 --> 00:12:14,750
+البروتين لو جينا على الصورة هذه على الخط اللي فوق
+
+163
+00:12:14,750 --> 00:12:17,830
+أول شيء إحنا عرفنا الصيغة العامة و ال structure
+
+164
+00:12:17,830 --> 00:12:21,050
+العامة للحمض الأميني إنه بيتكون من مجموعة carbon
+
+165
+00:12:21,050 --> 00:12:26,390
+مركزية مرتبطة بهيدروجين وبـ side chain وكربوكسيل
+
+166
+00:12:26,390 --> 00:12:32,070
+وأمين برون توجينها الخط اللي فوقها على رموز جيت
+
+167
+00:12:32,070 --> 00:12:36,810
+عنه أول خط أحمر فوق اللي هي ال repeat units فيه
+
+168
+00:12:36,810 --> 00:12:39,770
+عندنا صيغة العاملة للحمض الأميني فيه في النص
+
+169
+00:12:39,770 --> 00:12:44,870
+مجموعة كاربون من فوق مرتبط فيها مجموعة هيدروجين من
+
+170
+00:12:44,870 --> 00:12:47,530
+الأسفل مرتبط فيها الـ R group اللي هي الـ side
+
+171
+00:12:47,530 --> 00:12:51,890
+chain على اليمين مرتبط فيها الـ C double bond O
+
+172
+00:12:51,890 --> 00:12:56,890
+اللي هي الكربوكسيل group على اليسار مرتبط فيها NH
+
+173
+00:12:56,890 --> 00:13:01,510
+اللي هي مجموعة الأمين amino group تمام؟ هي أول
+
+174
+00:13:01,510 --> 00:13:06,420
+حمض أميني جنبه مرتبط فيه حمض أميني ثاني اللي هي الـ
+
+175
+00:13:06,420 --> 00:13:10,120
+Carbona المركزية و Hydrogen في الأعلى و R في
+
+176
+00:13:10,120 --> 00:13:13,720
+الأسفل وعلى اليمين Carboxyl group وعلى اليسار
+
+177
+00:13:13,720 --> 00:13:18,580
+Amino group هدول مجموعة أحماض أمينية
\ No newline at end of file
diff --git a/PL9fwy3NUQKwZ1Q7zOg1ObgufPMjRI3WY1/OOPghZ3VTGI_raw.srt b/PL9fwy3NUQKwZ1Q7zOg1ObgufPMjRI3WY1/OOPghZ3VTGI_raw.srt
new file mode 100644
index 0000000000000000000000000000000000000000..75518e492f936367a3844f87dac9a5816c436b6c
--- /dev/null
+++ b/PL9fwy3NUQKwZ1Q7zOg1ObgufPMjRI3WY1/OOPghZ3VTGI_raw.srt
@@ -0,0 +1,740 @@
+1
+00:00:00,000 --> 00:00:04,560
+الـ Solution هو جهاز لفصل المواد حسب الكثافة
+
+2
+00:00:04,560 --> 00:00:10,780
+الثقيل ينزل تحت والخفيف يكون في الأعلى جهاز لفصل
+
+3
+00:00:10,780 --> 00:00:14,580
+المواد بناء على الكثافة Separate material of
+
+4
+00:00:14,580 --> 00:00:19,000
+varying density تستيتيوط قنابيب نستخدمها عشان
+
+5
+00:00:19,000 --> 00:00:22,800
+نشتغل فيها الفحوصات نحط فيها small amount of
+
+6
+00:00:22,800 --> 00:00:28,880
+solution قنابيب تحتوي على أو تتسع إلى كميات قليلة
+
+7
+00:00:28,880 --> 00:00:33,930
+من المحلينThermometer بجيس فيه الحرارة measure
+
+8
+00:00:33,930 --> 00:00:39,670
+temperature وير جوز هي شبكة موزعة للحرارة وير جوز
+
+9
+00:00:39,670 --> 00:00:43,670
+شبكة موزعة للحرارة بحطها فوق اللهب بتعمل spread
+
+10
+00:00:43,670 --> 00:00:50,430
+heat بتوزع الحرارة اللي بيوريت هي الصحاحة كده
+
+11
+00:00:50,430 --> 00:00:54,430
+استخدمتوها في الكيميا اللي بنستخدمها في عمليات ال
+
+12
+00:00:54,430 --> 00:00:59,750
+titrationنحط فيها جواه سائل بنسميه titrant الـ
+
+13
+00:00:59,750 --> 00:01:04,330
+«بيريت» ساحةها تستخدم للمعايرة للـ titration تحتوي
+
+14
+00:01:04,330 --> 00:01:08,370
+على السائل في داخلها بنسميه titrant كيف بدي أقرأ
+
+15
+00:01:08,370 --> 00:01:13,550
+منها major volume نقرأ القراءة من سطح التقعر من
+
+16
+00:01:13,550 --> 00:01:19,220
+سطح التقعر كتم القراءةهنا حاجة بنتسميها certainty
+
+17
+00:01:19,220 --> 00:01:22,700
+و حاجة بنتسميها uncertainty اللي هي القيمة اللي
+
+18
+00:01:22,700 --> 00:01:26,820
+أنا متأكدة منها طويق مشاك فيها هو أنا مثلا في
+
+19
+00:01:26,820 --> 00:01:30,260
+البيوت في الصحاحة بدي أجي أقرأ ال volume حكي أنا
+
+20
+00:01:30,260 --> 00:01:33,560
+بنقرأ من سطح التقاعو لو جينا شوفنا الصورة اللي على
+
+21
+00:01:33,560 --> 00:01:38,000
+الجنب في ال slide هلجيت القراءة جاي فوق ال 36
+
+22
+00:01:38,000 --> 00:01:43,750
+بشويةتمام؟ هي هي 35 الشرطة اللي فوقها 36 هي بعيدها
+
+23
+00:01:43,750 --> 00:01:48,510
+بشوية يعني القيمة القراعة هتكون 36 و إشي ممكن
+
+24
+00:01:48,510 --> 00:01:51,810
+واحدة تيجي تقولي إنها 36 و نصف واحدة تانية تقولي
+
+25
+00:01:51,810 --> 00:01:56,770
+36 و 6 واحدة تقولي 36 و 4 لكن القيمة اللي أنا
+
+26
+00:01:56,770 --> 00:02:01,320
+متأكدة منها ال certainty إنها 36والقيمة اللى فيها
+
+27
+00:02:01,320 --> 00:02:05,040
+الشك اللى هيتختلف من one ل two اللى هى الأعشار
+
+28
+00:02:05,040 --> 00:02:08,320
+اللى جنبها اللى هى الخمسة من عشرة ستة من عشرة
+
+29
+00:02:08,320 --> 00:02:14,400
+أربعة من عشرة فالفرق بيكون زائد أو ناقص واحد من
+
+30
+00:02:14,400 --> 00:02:18,720
+عشرة هاي بتكون القيمة ال uncertaintyTamam
+
+31
+00:02:18,720 --> 00:02:21,400
+certainty اللي أنا متأجدة منها في الصورة مثلا
+
+32
+00:02:21,400 --> 00:02:26,000
+للقراءة هاي أنها 36 Uncertainty هي الأعشار اللي
+
+33
+00:02:26,000 --> 00:02:29,100
+جنبها، اللي هو أنا .. هي مش على ال 36 بالظبط، هي
+
+34
+00:02:29,100 --> 00:02:33,000
+فوقها في شويه، يعني ممكن تكون 36 و نص، ممكن 36 و
+
+35
+00:02:33,000 --> 00:02:38,350
+6، ممكن 36 و أعملالـ Certainty بتعتمد على حاجة
+
+36
+00:02:38,350 --> 00:02:42,370
+بنسميها precision على الدقة الـ Certainty و الـ
+
+37
+00:02:42,370 --> 00:02:45,890
+Measurement depends on the precision بتعتمد على
+
+38
+00:02:45,890 --> 00:02:50,010
+الدقة يعني الدقة مثلا في الأدوات اللي مقسمة أكتر
+
+39
+00:02:50,010 --> 00:02:54,410
+هتكون الدقة أكتر فيها مجسمة لا عشار ولا مجسمة بس
+
+40
+00:02:54,410 --> 00:02:58,650
+لعداد صحيحة مين هتكون أدق؟ أكيد اللي مجسمة لا عشار
+
+41
+00:02:58,650 --> 00:02:59,710
+هي الأدق
+
+42
+00:03:02,280 --> 00:03:06,680
+هنا مصطلحين مهمين اللي هم بتسميهم accuracy و
+
+43
+00:03:06,680 --> 00:03:11,220
+precision تجي نتعرف على accuracy ماذا تعني؟
+
+44
+00:03:11,220 --> 00:03:16,000
+accuracy تعني مدى قرب النتيجة من النتيجة الحقيقية
+
+45
+00:03:16,000 --> 00:03:20,900
+درجة الانتباه من الحقيقة ال degree of conformity
+
+46
+00:03:20,900 --> 00:03:26,080
+to the truth and experiment مدى قرب النتيجة من
+
+47
+00:03:26,080 --> 00:03:31,950
+النتيجة الحقيقيةكيف يعني؟ يعني انا بحكيلك ان فيها
+
+48
+00:03:31,950 --> 00:03:36,290
+نفاح السكر اشتغلت و طلعت مية خد اشتغليها و شوفيها
+
+49
+00:03:36,290 --> 00:03:39,710
+قيمة الحقيقية انا حكيتلك انها مية و انت اشتغلتيها
+
+50
+00:03:39,710 --> 00:03:44,070
+و طلعت معاكي سبعين هل السبعين قريبة من المية؟ لأ
+
+51
+00:03:44,070 --> 00:03:47,890
+فهي بنسميها inaccurate result انت ما طلعتيهاش
+
+52
+00:03:47,890 --> 00:03:51,750
+accurate لكن لو طلعت معاكي تسعة و تسعين تسعة و
+
+53
+00:03:51,750 --> 00:03:55,770
+تسعين قريبة من المية اذا accurate result accuracy
+
+54
+00:03:55,770 --> 00:04:00,820
+مدى قرب النتيجة من النتيجة الحقيقيةفبالـ Precision
+
+55
+00:04:00,820 --> 00:04:05,080
+الـ Precision هي مادة قُرب النتائج من بعضها البعض
+
+56
+00:04:05,080 --> 00:04:09,380
+degree of agreement between replicated experiments
+
+57
+00:04:12,600 --> 00:04:17,340
+وطلعت النتاج في كل مرة يعني نفس الفحص اشتغلته خمس
+
+58
+00:04:17,340 --> 00:04:21,900
+مرات تمام؟ طلعت معايا القيمة الأولى 72 القيمة
+
+59
+00:04:21,900 --> 00:04:28,500
+التانية 74 القيمة التالتة 71 القيمة الرابعة 73 هل
+
+60
+00:04:28,500 --> 00:04:31,980
+هما قريبين من بعض؟ هو كلهم قريبين من بعض فهي
+
+61
+00:04:31,980 --> 00:04:37,180
+precise result نتائجك كانت precisePrecision مدى
+
+62
+00:04:37,180 --> 00:04:40,760
+قربها من بعضها البعض إن أنا أعيد الفحص أكتر من مرة
+
+63
+00:04:40,760 --> 00:04:44,560
+و أشوف النتائج هل قريبة من بعض ولا لا Accuracy قرب
+
+64
+00:04:44,560 --> 00:04:47,500
+النتيجة من النتيجة الحقيقية أنا بكون معطياكي
+
+65
+00:04:47,500 --> 00:04:51,100
+النتيجة و أنت اشتغليها هل طلعت نتيجتي مقاربة
+
+66
+00:04:51,100 --> 00:04:55,280
+لنتيجتي فهنا بتكون accurate result إذا طلعت بعيد
+
+67
+00:04:55,280 --> 00:05:00,780
+عنها بتكون not accurate، inaccurate result طبعا؟
+
+68
+00:05:00,780 --> 00:05:05,000
+طب أنا حكيتلك إنه عندي أنا فحص اشتغلت و طلعت 100
+
+69
+00:05:05,760 --> 00:05:09,160
+وانت اشتغلي ليها اكتر من مرة وشوفي ليها accurate
+
+70
+00:05:09,160 --> 00:05:13,460
+or precise اشتغلتي انا وحكيتلك انها 100، انت
+
+71
+00:05:13,460 --> 00:05:19,020
+اشتغلتيها خمس مرة، طلعت معاكي مرة 101، مرة 99، مرة
+
+72
+00:05:19,020 --> 00:05:24,380
+102، مرة 98، هلجيت النتائج قريبة من بعض، كانت 101،
+
+73
+00:05:24,380 --> 00:05:31,000
+99، 102، 98ما أنها قريبة من بعض هتكون precise طب
+
+74
+00:05:31,000 --> 00:05:34,520
+النتيجة الحقيقية كانت مية وكانوا جراب على المية
+
+75
+00:05:34,520 --> 00:05:38,700
+كلهم، لذا هي precise و accurate جمعت من الإتنين
+
+76
+00:05:38,700 --> 00:05:42,080
+كانت precise و accurate مع بعض
+
+77
+00:05:45,090 --> 00:05:48,870
+نحكي عن الـ chemical reaction التفاعل الكيميائي
+
+78
+00:05:48,870 --> 00:05:54,390
+التفاعل الكيميائي هو تفاعل تدخل فيه مادة تطلع عنها
+
+79
+00:05:54,390 --> 00:06:00,210
+مادة جديدة بصفات جديدة تغيير مادتك إلى مادة جديدة
+
+80
+00:06:00,210 --> 00:06:03,310
+بخصائص
+
+81
+00:06:03,310 --> 00:06:08,410
+مختلفة تدخل مادة جديدة بخصائص مختلفةSign of
+
+82
+00:06:08,410 --> 00:06:13,110
+chemical reaction علامات حدوث التفاعل أول شيء ممكن
+
+83
+00:06:13,110 --> 00:06:16,950
+يتكون عندي راسم هذا يدل على حدوث التفاعل Formation
+
+84
+00:06:16,950 --> 00:06:20,910
+of precipitate Color change ممكن يتغير اللون
+
+85
+00:06:20,910 --> 00:06:26,070
+Evolution of gas يعني تصاعد غاز تصاعد غاز يعني
+
+86
+00:06:26,070 --> 00:06:30,130
+الحدث عندي تغير Temperature change تتغير درجة
+
+87
+00:06:30,130 --> 00:06:35,120
+الحرارة برضه هو علامة من علامات حدوث التفاعلكيف
+
+88
+00:06:35,120 --> 00:06:38,720
+تتغير درجة الحرارة؟ في عندنا نوعين من التفاعل في
+
+89
+00:06:38,720 --> 00:06:44,020
+تفاعل اسمه Exothermic Reaction يعني طارد للحرارة
+
+90
+00:06:44,020 --> 00:06:48,640
+يعني بيحتاجش الحرارة، بيطلع الحرارة لبرا فبتكون
+
+91
+00:06:48,640 --> 00:06:52,280
+الأنبوبة لما تحسيها من برا بتكون ساخنةto get
+
+92
+00:06:52,280 --> 00:06:56,140
+hotter surrounding to get hot هوته يكون الخارج
+
+93
+00:06:56,140 --> 00:07:00,520
+المحيط الخارجي ساخن لأنه طارد للحرارة endothermic
+
+94
+00:07:00,520 --> 00:07:05,860
+reaction ماصل الحرارة تفاعل ماصل الحرارة تحتاج
+
+95
+00:07:05,860 --> 00:07:10,560
+لحرارة بتاخد حرارة فبيكون المحيط الخارجي بارد cool
+
+96
+00:07:13,910 --> 00:07:17,050
+عشان معاها ال biochemical compound اللى هنتعرف
+
+97
+00:07:17,050 --> 00:07:21,190
+عليها هنتعرف على ال carbohydrates على ال amino
+
+98
+00:07:21,190 --> 00:07:25,130
+acid و ال protein هنتعرف على ال lemon نيوكليك
+
+99
+00:07:25,130 --> 00:07:31,180
+تايبز والنيوكليك أسد هتاخدوها في مادة تانيةبدنا
+
+100
+00:07:31,180 --> 00:07:35,400
+اليه ندخل في مقدمة بسيطة عن الـ amino acid الأحماض
+
+101
+00:07:35,400 --> 00:07:39,220
+الأمينية وإن شاء الله المرة اللي جاي هنكملها هذا
+
+102
+00:07:39,220 --> 00:07:44,440
+الموضوع الأحماض الأمينية الـ amino acid إيش هي؟ هي
+
+103
+00:07:44,440 --> 00:07:48,880
+الـ building blocks of البروتين هي الوحدة البنائية
+
+104
+00:07:48,880 --> 00:07:53,760
+لتركيب البروتين تكون مرتبطة مع بعضها linked to
+
+105
+00:07:53,760 --> 00:07:58,240
+peptide Iأحماد أمينية مجموعة أحماد أمينية بتكون
+
+106
+00:07:58,240 --> 00:08:02,420
+مرتبطة مع بعض في peptide bond في رابطة بيبتيدية
+
+107
+00:08:02,420 --> 00:08:06,680
+لما يرتبطوا مع بعض هدول بيكونولي البروتين فالحمض
+
+108
+00:08:06,680 --> 00:08:12,180
+الأميني هو الوحدة البنائية لتركيب البروتين وترتبط
+
+109
+00:08:12,180 --> 00:08:17,930
+مع بعضها بروابط بيبتيدية بـ peptide bondإيش تركيب
+
+110
+00:08:17,930 --> 00:08:21,690
+هذا الحمض الأمين؟ لو نطلع على الصورة اللي عندنا
+
+111
+00:08:21,690 --> 00:08:26,530
+كيف تركيبها بتكون بالنص في عندنا ذرة Carbon هذه
+
+112
+00:08:26,530 --> 00:08:30,090
+الـCarbon بنسميها Central Carbon اللي هي الكربون
+
+113
+00:08:30,090 --> 00:08:34,970
+المركزية أو إلها اسم تاني Alpha Carbon أو اسمها
+
+114
+00:08:34,970 --> 00:08:38,900
+التالت Primary Carbonهي عندنا في الوسط في تركيب
+
+115
+00:08:38,900 --> 00:08:41,660
+الحمض الأميني بتكون عندنا الـ Central Carbon
+
+116
+00:08:41,660 --> 00:08:46,960
+كربونا مركزية او ايه تسميها تاني Alpha Carbon او
+
+117
+00:08:46,960 --> 00:08:50,900
+Primary Carbon هاي الـ Central Carbon بتكون مرتبطة
+
+118
+00:08:50,900 --> 00:08:56,080
+باربع روابط بتكون مرتبطة بجهة بمجموعة بنسميها
+
+119
+00:08:56,080 --> 00:09:01,950
+Carboxyl Group Carboxyl Group اللي هي COOHالـ
+
+120
+00:09:01,950 --> 00:09:06,050
+Carboxyl group و بتكون من جهة التانية مرتبطة
+
+121
+00:09:06,050 --> 00:09:11,050
+بمجموعتها .. بضارة هيدروجين بـ H و من جهة بتكون
+
+122
+00:09:11,050 --> 00:09:15,330
+مرتبطة بـ Aminogroup لأن H3I plus بسميها
+
+123
+00:09:15,330 --> 00:09:20,150
+Aminogroup و من الأسفل بتكون مرتبطة بالـ R group
+
+124
+00:09:20,150 --> 00:09:24,890
+الـ R group هاي اسمها الـ sided chainالـ side
+
+125
+00:09:24,890 --> 00:09:28,970
+chain هذه هي اللي بتميز الحمض الأميني عن غيره هي
+
+126
+00:09:28,970 --> 00:09:32,330
+عبارة عن مجموعة بتكون مرتبطة ممكن تكون ذرة واحدة
+
+127
+00:09:32,330 --> 00:09:37,250
+وممكن تكون مجموعةهي اللي بتميز كل حمض أميني عن
+
+128
+00:09:37,250 --> 00:09:41,290
+الآخر كل الأحماض الأمينية لها نفس التركيب بتكون
+
+129
+00:09:41,290 --> 00:09:45,730
+ذرة كربون و هيدروجين و كربوكسيل و أمينو و الـR
+
+130
+00:09:45,730 --> 00:09:49,590
+group هذي هي اللي بتختلف من حمض أميني للآخر يعني
+
+131
+00:09:49,590 --> 00:09:54,570
+اللي بيميز حمض أميني عن الآخر هي الـR group تمام؟
+
+132
+00:09:55,130 --> 00:09:59,930
+وكل الأحماض الأمينية عندها amino group او primary
+
+133
+00:09:59,930 --> 00:10:03,650
+amino group اللي هي مجموعة الأمين العجبى بنسميها
+
+134
+00:10:03,650 --> 00:10:10,130
+amino group او primary amino group ماعدى حمض أمين
+
+135
+00:10:10,130 --> 00:10:14,750
+واحد اسمه البرولين البرولين ماعندهوش amino عنده
+
+136
+00:10:14,750 --> 00:10:19,830
+حاجة بنسميها amino group او secondary amino group
+
+137
+00:10:21,080 --> 00:10:26,960
+كلهم عندهم amino group مع ده البرولين عنده amino
+
+138
+00:10:26,960 --> 00:10:31,480
+group أو secondary amino group ليش سموها هكذا؟
+
+139
+00:10:31,480 --> 00:10:34,920
+عشان مجموعة ال amino هاي اللي على الجنب الـ NH3
+
+140
+00:10:34,920 --> 00:10:38,880
+plus بتكون مرتبطة مع الـR و مع الـC عاملة حلقة
+
+141
+00:10:38,880 --> 00:10:44,210
+بينها و بين نفسهاتكون الـ Amino Group مرتبطة مع
+
+142
+00:10:44,210 --> 00:10:47,550
+الـ R من جهة مع الـ Side Chain ومع الكربون
+
+143
+00:10:47,550 --> 00:10:50,910
+المركزية من جهة، متكونش مربوطة برابطة واحدة مع
+
+144
+00:10:50,910 --> 00:10:54,090
+الكربون زي الصورة وخلاص، لأ، بتكون مربوطة من
+
+145
+00:10:54,090 --> 00:10:58,390
+جهتها، جهة مع الكربون وجهة مع الـ R، مع الـ Side
+
+146
+00:10:58,390 --> 00:11:03,030
+Chain، هنا ماضلتش اسمها Primary Amino Group زي أي
+
+147
+00:11:03,030 --> 00:11:06,410
+حمض أميني مرتبطة بجهة واحدة، ساميها Primary، زي
+
+148
+00:11:06,410 --> 00:11:10,910
+مرتبطة في الكربونة وخلاصإذا مرتبطة مع الكربونة ومع
+
+149
+00:11:10,910 --> 00:11:15,690
+الـR زي في حالة لبرولين وهو الحالة الوحيدة الحمض
+
+150
+00:11:15,690 --> 00:11:21,790
+الوحيد بنتسميها amino group أو secondary amino
+
+151
+00:11:21,790 --> 00:11:28,130
+groupفي عندنا الـ food are divided into three
+
+152
+00:11:28,130 --> 00:11:31,510
+classes طعام مقسم إلى تلاتة في عندنا carbohydrate
+
+153
+00:11:31,510 --> 00:11:35,310
+و lipid و protein طعام بتكون من تلاتة
+
+154
+00:11:35,310 --> 00:11:39,270
+carbohydrate، lipid و protein ال carbohydrate
+
+155
+00:11:39,270 --> 00:11:43,770
+تعتبر هي مصدر الطاقة source of energy ال lipid
+
+156
+00:11:43,770 --> 00:11:49,250
+تعتبر مخزن الطاقة energy reserve ال protein هي
+
+157
+00:11:49,250 --> 00:11:53,830
+energy for growth and cellular maintenanceهي عبارة
+
+158
+00:11:53,830 --> 00:11:59,190
+عن طاقة الخلايا المستخدمة للنمو لبناء الجسم إذا
+
+159
+00:11:59,190 --> 00:12:03,410
+عندنا تلاتة نجد إحنا حكينا إنه البروتين كيف بتكون
+
+160
+00:12:03,410 --> 00:12:07,550
+بتيجي مجموعة أحماض أمينية ترتبط مع بعضها البعض
+
+161
+00:12:07,550 --> 00:12:11,450
+بيهرتبط high point في روابط بيبتدية بتكوين
+
+162
+00:12:11,450 --> 00:12:14,750
+البروتين لو جينا على الصورة هذه على الخط اللي فوق
+
+163
+00:12:14,750 --> 00:12:17,830
+أول شيء احنا عرفنا الصيغة العامة و ال structure
+
+164
+00:12:17,830 --> 00:12:21,050
+العامة للحمض الأميني إنه بيتكون من مجموعة carbon
+
+165
+00:12:21,050 --> 00:12:26,390
+مركزيةمرتبطة بهيدروجين وباردشين وكربوكسين
+
+166
+00:12:26,390 --> 00:12:32,070
+وامينوبرون توجينها الخط اللي فوقها على رموز جيت
+
+167
+00:12:32,070 --> 00:12:36,810
+عنه أول خط أحمر فوق اللي هي ال repeat units فيه
+
+168
+00:12:36,810 --> 00:12:39,770
+عندنا صيغة العاملة الحمد الأميني فيه في النص
+
+169
+00:12:39,770 --> 00:12:44,870
+مجموعة كاربون من فوق مرتبط فيها مجموعة هيدروجينمن
+
+170
+00:12:44,870 --> 00:12:47,530
+الأسفل مرتبط فيها الـ R group اللي هي الـ side
+
+171
+00:12:47,530 --> 00:12:51,890
+chain على اليمين مرتبط فيها الـ C double bond O
+
+172
+00:12:51,890 --> 00:12:56,890
+اللي هي الكربوكسيل group على اليسار مرتبط فيها NH
+
+173
+00:12:56,890 --> 00:13:01,510
+اللي هي مجموعة الأمين الأمينو group تمام؟ هي أول
+
+174
+00:13:01,510 --> 00:13:06,420
+حمض أميني جنبه مرتبط فيه حمض أميني تاني اللي هيالـ
+
+175
+00:13:06,420 --> 00:13:10,120
+Carbona المركزية و Hydrogen في الأعلى و R في
+
+176
+00:13:10,120 --> 00:13:13,720
+الأسفل وعلى اليمين Carboxyl group وعلى الجمال
+
+177
+00:13:13,720 --> 00:13:18,580
+Amino group هدول مجموعة أحماض أمينية أجت اترتبت
+
+178
+00:13:18,580 --> 00:13:22,860
+جانب بعض مرتبطة مع بعضها البعض في peptide bond
+
+179
+00:13:22,860 --> 00:13:28,460
+رابطة ببتدية فكونتلي البروتين طب وين ال peptide
+
+180
+00:13:28,460 --> 00:13:33,820
+bond جاية؟ ال peptide bond أجت وارتبطت بيهمجموعة
+
+181
+00:13:33,820 --> 00:13:38,480
+كربوكسيل من الحمض الأمين الأول ومجموعة أمين من
+
+182
+00:13:38,480 --> 00:13:42,600
+الحمض الأمين التاني إذا ملاحظين الحمض الأمين الأول
+
+183
+00:13:42,600 --> 00:13:46,880
+و الحمض الأمين التاني في بينهم رابطة هذه الرابطة
+
+184
+00:13:46,880 --> 00:13:49,440
+اللي في النص اللي فصلت الحمض الأمين الأول عن
+
+185
+00:13:49,440 --> 00:13:52,000
+التاني بنسميها peptide bond
+
diff --git a/PL9fwy3NUQKwZ1Q7zOg1ObgufPMjRI3WY1/OzyPnleVow8.srt b/PL9fwy3NUQKwZ1Q7zOg1ObgufPMjRI3WY1/OzyPnleVow8.srt
new file mode 100644
index 0000000000000000000000000000000000000000..c92af408641f0bc3fb87b60df0b623b49a3ff3cf
--- /dev/null
+++ b/PL9fwy3NUQKwZ1Q7zOg1ObgufPMjRI3WY1/OzyPnleVow8.srt
@@ -0,0 +1,535 @@
+1
+00:00:00,540 --> 00:00:04,000
+العمل اللي فاد هيك من العوامل اللي بتأثر على نشاط
+
+2
+00:00:04,000 --> 00:00:07,120
+الانزيم هو الـInhibitors، Inhibitors سمعناها
+
+3
+00:00:07,120 --> 00:00:10,740
+المطبطّات، Inhibitors are chemicals that reduce the
+
+4
+00:00:10,740 --> 00:00:16,060
+rate of enzymatic reaction هي مواد بتقلل معدل حدوث
+
+5
+00:00:16,060 --> 00:00:20,140
+التفاعل الكيميائي وبتقلل نشاط الانزيم، They are
+
+6
+00:00:20,140 --> 00:00:22,880
+usually specific and they work at low
+
+7
+00:00:22,880 --> 00:00:28,890
+concentration، هاي الـInhibitors هي متخصصة،
+
+8
+00:00:28,890 --> 00:00:33,150
+specific، كل Inhibitor متخصص لأنزيم معين، يعني في
+
+9
+00:00:33,150 --> 00:00:35,730
+أن الـinhibitor بيخبط الانزيم مثلا على الكلاين
+
+10
+00:00:35,730 --> 00:00:39,170
+الفسفتان، عن الـInhibitor تاني لأنزيم تاني، كل 
+
+11
+00:00:39,170 --> 00:00:43,730
+Inhibitor بيخبط انزيم معين، يعني متخصص، specific،
+
+12
+00:00:43,730 --> 00:00:46,370
+they block the enzyme but they don't usually 
+
+13
+00:00:46,370 --> 00:00:51,290
+destroy it، بيعمل الـInhibitor على إنه يوقف نشاط
+
+14
+00:00:51,290 --> 00:00:56,470
+الانزيم بدون ما يدمر الانزيم، بس بيوقف نشاط الانزيم 
+
+15
+00:00:56,470 --> 00:01:02,430
+بدون ما يعمل تدمير للانزيم، إيش أنواع الـInhibitors
+
+16
+00:01:02,430 --> 00:01:05,830
+الموجودين؟ في عندنا reversible inhibitor أو 
+
+17
+00:01:05,830 --> 00:01:08,870
+irreversible، نوعين، reversible، irreversible 
+
+18
+00:01:08,870 --> 00:01:12,430
+الـreversible inhibitor هو نفسه الـcompetitive
+
+19
+00:01:12,430 --> 00:01:17,670
+inhibitor، reversible هو competitive inhibitor، كيف
+
+20
+00:01:17,670 --> 00:01:21,890
+هدا بيشتغل؟ هدا competitive، تنافسين، بتيجي
+
+21
+00:01:21,890 --> 00:01:26,190
+بتتنافس الـsubstrate والـinhibitor، إنه بيتنافس
+
+22
+00:01:26,190 --> 00:01:30,830
+الـsubstrate والـinhibitor على الارتباط بالـactive
+
+23
+00:01:30,830 --> 00:01:34,470
+site، فجهة الـsubstrate، حكينا إنه بيرتبط مع
+
+24
+00:01:34,470 --> 00:01:37,770
+الانزيم في موقع اسمه الـactive site، عشان يشتغل،
+
+25
+00:01:38,290 --> 00:01:41,570
+بتيجي هاي الـInhibitor بتتنافس مع الـsubstrate على
+
+26
+00:01:41,570 --> 00:01:45,030
+الـactive side، إنه هاي الـInhibitor إلها شكل شبه
+
+27
+00:01:45,030 --> 00:01:48,150
+شكل الـactive side، زيها زي الـsubstrate، فهي
+
+28
+00:01:48,150 --> 00:01:51,310
+بتتنافس هي و الـsubstrate على هذا الموقع، إنه
+
+29
+00:01:51,310 --> 00:01:53,130
+الـInhibitor بيقول لها الـsubstrate أنا بدي
+
+30
+00:01:53,130 --> 00:01:56,570
+أرتبط فيها، و الـsubstrate بيحكي لأ هذا مكان يعني أنا
+
+31
+00:01:56,570 --> 00:02:00,310
+بدي أرتبط فيه، هم الإتنين بدهم هذا المكان، طب مين
+
+32
+00:02:00,310 --> 00:02:05,100
+هيفوز؟ بيحكي للي إله أعلى تركيز هو اللي هيفوز، إذا
+
+33
+00:02:05,100 --> 00:02:08,320
+الـ Substrate تركيزها أعلى هي اللي هتفوز وهترتبط 
+
+34
+00:02:09,140 --> 00:02:12,580
+اللي له أقل تركيز هو اللي مش هيرتبط، إذا كان
+
+35
+00:02:12,580 --> 00:02:16,120
+الـInhibitor تركيزه أعلى، هيرتبط، إذا الـSubstrate
+
+36
+00:02:16,120 --> 00:02:19,060
+هي اللي تركيزها أعلى، هي اللي هترتبط، طيب، أنا لو
+
+37
+00:02:19,060 --> 00:02:22,420
+عندي مشكلة إنه عندي Competitive Inhibitor موجود
+
+38
+00:02:22,420 --> 00:02:26,640
+كيف بدي أحل هذه المشكلة عشان أتلاشى و أبعد عن هذا
+
+39
+00:02:26,640 --> 00:02:29,860
+الـInhibitor و أخلي بس الـSubstrate يرتبط؟ سهل،
+
+40
+00:02:29,860 --> 00:02:33,420
+إني أضيف تركيز الـSubstrate بزيادة، أضيف على
+
+41
+00:02:33,420 --> 00:02:38,470
+التفاعل Substrate، أضمن أضيف Substrate، فبيصير تركيز
+
+42
+00:02:38,470 --> 00:02:41,870
+الـ substrate أعلى من الـ inhibitor فهو بيفوز
+
+43
+00:02:41,870 --> 00:02:45,730
+وبيرتبط بدل الـ inhibitor، then these compete with
+
+44
+00:02:45,730 --> 00:02:48,910
+the substrate molecule for active site، بيتنافس مع
+
+45
+00:02:48,910 --> 00:02:52,530
+ال substrate علاش على الactive site، الـ Inhibitor
+
+46
+00:02:52,530 --> 00:02:55,730
+actually proportional to its concentration، إن
+
+47
+00:02:55,730 --> 00:03:00,570
+تركيز الـ Inhibitor بيعتمد ما عمله، إنه كل ما زاد
+
+48
+00:03:00,570 --> 00:03:04,230
+تركيز الـ Inhibitor كل ما زاد ارتباطه بسبب الـ
+
+49
+00:03:04,230 --> 00:03:07,990
+enzyme في الـ active site وهو اللي هيفوز، لكن كل ما
+
+50
+00:03:07,990 --> 00:03:12,730
+قل تركيز الـ Inhibitor بيقل الارتباط الـ inhibitor
+
+51
+00:03:12,730 --> 00:03:17,040
+مع الـ enzyme، come over this problem by adding more
+
+52
+00:03:17,040 --> 00:03:20,980
+substrate، كيف بدي أحل المشكلة دي و أتلافق مشكلة
+
+53
+00:03:20,980 --> 00:03:24,920
+وجود competitive inhibitor، أني أضيف substrate بحيث
+
+54
+00:03:24,920 --> 00:03:28,840
+أن يكون تركيز ال substrate أعلى من تركيز ال
+
+55
+00:03:28,840 --> 00:03:33,250
+inhibitor، طب في عندنا مخطط على الجانب بيوضحلي إنه كيف
+
+56
+00:03:33,250 --> 00:03:36,730
+هيمشي التفاعل في وجود «no inhibitor» وفي حال وجود
+
+57
+00:03:36,730 --> 00:03:40,510
+«competitive inhibitor»، هلجيت في الحالتين سواء في
+
+58
+00:03:40,510 --> 00:03:45,130
+Inhibitor أو فيش Inhibitor أنا هاصل لأقصى سرعة من
+
+59
+00:03:45,130 --> 00:03:49,750
+التفاعل الكيميائي، ولكن هل هامشي بنفس السرعة ولا لأ
+
+60
+00:03:49,750 --> 00:03:53,010
+في الحالتين، في وجود Inhibitor أو في عدم وجود
+
+61
+00:03:53,010 --> 00:03:56,830
+Inhibitor أنا هاصل الـVmax، هاصل لأقصى سرعة من
+
+62
+00:03:56,830 --> 00:04:00,460
+التفاعل، لكن الحين لو فيش الـInhibitor، هيمشي التفاعل
+
+63
+00:04:00,460 --> 00:04:03,820
+عادي، هيوصل لأقصى سرعة من التفاعل، عند أقصى
+
+64
+00:04:03,820 --> 00:04:07,220
+السرعة، بتتشبع كل الانزيم بالـSubstrate، بيصير عندنا
+
+65
+00:04:07,220 --> 00:04:11,440
+Saturation Point ونقطة التشبع، فهيثبت المنحنى، هدا
+
+66
+00:04:11,440 --> 00:04:15,320
+في عدم وجود Inhibitor، أنا أوصل فيه Max، تمام؟ هذا
+
+67
+00:04:15,320 --> 00:04:18,560
+حكينا عنه قبلها، بيبقى عدم وجود Inhibitor، حكينا
+
+68
+00:04:18,560 --> 00:04:21,600
+كيف بيشتغل الانزيم إنه كل ما زاد تركيز الـ
+
+69
+00:04:21,600 --> 00:04:25,740
+Substrate بتزيد سرعة التفاعل وصولا إلى الـ
+
+70
+00:04:25,740 --> 00:04:29,320
+saturation point، إلى نقطة التشبع، يعني نقطة
+
+71
+00:04:29,320 --> 00:04:33,160
+التشبع بيثبت المنحنى لأنه خلاص كل الانزيمات
+
+72
+00:04:33,160 --> 00:04:36,440
+متشبعة في الـ Substrate ففيش زيادة لسرعة التفاعل
+
+73
+00:04:36,440 --> 00:04:40,420
+تقنيجي لو عدم وجود Competitive Inhibitor، في حال
+
+74
+00:04:40,420 --> 00:04:44,690
+وجود، وجود Competitive Inhibitor، في حال وجود الـ
+
+75
+00:04:44,690 --> 00:04:47,970
+Competitive Inhibitor حكينا إنه أنا بدي أوصل
+
+76
+00:04:47,970 --> 00:04:51,730
+لـVmax لأقصى سرعة من التفاعل، لكن كيف بدي أشيل هذا
+
+77
+00:04:51,730 --> 00:04:55,910
+الـInhibitor؟ بدي أزود الـSubstrate، مين اللي بيعبر
+
+78
+00:04:55,910 --> 00:04:58,750
+عن تركيز الـSubstrate، حكينا اللي هي عبارة عن تركيز
+
+79
+00:04:58,750 --> 00:05:03,240
+الـSubstrate؟ هي KM، مش حكينا «KM»، هي تركيز الـ
+
+80
+00:05:03,240 --> 00:05:07,180
+«Substrate» عن نص «Vmax»، فهي بتعبّر عن تركيز الـ
+
+81
+00:05:07,180 --> 00:05:11,100
+«Substrate»، فأنا مين هزود؟ هل هلعب في «Vmax»؟ هزود
+
+82
+00:05:11,100 --> 00:05:14,520
+«Vmax»؟ لأ، أنا بدي أزود تركيز الـ «Substrate»
+
+83
+00:05:14,520 --> 00:05:19,100
+فبدي أزود «KM»، إذا في وجود الـ «Inhibitor» «KM»
+
+84
+00:05:19,100 --> 00:05:24,380
+هتتغير، هتزيد، إيش هيصير عليها؟ هتزيد «KM» في وجود
+
+85
+00:05:24,380 --> 00:05:28,560
+الـ «Inhibitor» هزيد «Km»، «Km» increase لكن «Vmax»
+
+86
+00:05:28,560 --> 00:05:33,440
+هل هتتأثر؟ لأ، هوصل لـ «Vmax»، فـ «Vmax» نفسها ما
+
+87
+00:05:33,440 --> 00:05:38,760
+هتتغير، مش هتتغير وإنما مين اللي هيتغير بس «Km»،
+
+88
+00:05:38,760 --> 00:05:44,790
+تركيز الـ «substrate»، هنا إيش مكتوب؟ KM increase
+
+89
+00:05:44,790 --> 00:05:48,110
+بتزيد KM، طب ليش؟ لأن addition of competitive
+
+90
+00:05:48,110 --> 00:05:52,870
+inhibitor increased KM for a given substrate، في
+
+91
+00:05:52,870 --> 00:05:56,870
+حال وجود competitive inhibitor بتزيد KM عشان أزود
+
+92
+00:05:56,870 --> 00:06:00,610
+تركيز الـsubstrate عشان الـsubstrate يفوز ويكون
+
+93
+00:06:00,610 --> 00:06:04,130
+تركيزه أعلى من الـcompetitive inhibitor وهو اللي
+
+94
+00:06:04,130 --> 00:06:09,200
+يفوز في موقع الـactive site في الارتباط، في حالة الـ
+
+95
+00:06:09,200 --> 00:06:11,280
+Competitive Inhibitor يحتاج المزيد من الـ
+
+96
+00:06:11,280 --> 00:06:15,520
+Substrate لإيقافها، لوجود Competitive Inhibitor أنا
+
+97
+00:06:15,520 --> 00:06:18,500
+بحتاج إلى مزيد من الـ Substrate عشان يرتبط مع
+
+98
+00:06:18,500 --> 00:06:22,820
+النظيم وهو اللي يفوز و يمشي التفاعل و يوصل فيما
+
+99
+00:06:24,530 --> 00:06:28,810
+أما Vmax ما تتغير، سرعة التفاعل، أقصى سرعة التفاعل
+
+100
+00:06:28,810 --> 00:06:32,670
+مش هتتغير، هي هي في حال وجود inhibitor أو في عدم
+
+101
+00:06:32,670 --> 00:06:36,510
+وجود inhibitor، don't competitive inhibitor don't
+
+102
+00:06:36,510 --> 00:06:40,610
+alter the Vmax، مابيغيرش Vmax، effect of competitive
+
+103
+00:06:40,610 --> 00:06:43,970
+inhibitor is reversed by increasing substrate
+
+104
+00:06:43,970 --> 00:06:47,590
+concentration، high substrate concentration the
+
+105
+00:06:47,590 --> 00:06:51,870
+reaction velocity reached the same Vmax as that
+
+106
+00:06:51,870 --> 00:06:57,340
+observed in the absence of the inhibitor، محكي تأثير
+
+107
+00:06:57,340 --> 00:07:01,180
+الـ Competitive Inhibitor بقدر إني أتلاشى وإني
+
+108
+00:07:01,180 --> 00:07:05,440
+أشيلهم من خلال زيادة تركيز الـSubstrate، إذا زودت
+
+109
+00:07:05,440 --> 00:07:09,540
+تركيز الـSubstrate، التفاعل بيوصل لـVmax نفس اللي
+
+110
+00:07:09,540 --> 00:07:14,190
+كانت في الأول، بيوصل لأقصى سرعة من التفاعل، في اللي
+
+111
+00:07:14,190 --> 00:07:17,550
+هي نفس السرعة في حال غياب الـInhibitor، هاصل
+
+112
+00:07:17,550 --> 00:07:21,430
+الـVmax سواء فيه Inhibitor ولا فاشر، بس في حال وجود
+
+113
+00:07:21,430 --> 00:07:25,110
+Inhibitor لازم أزود تركيز الـSubstrate عشان أحصل
+
+114
+00:07:25,110 --> 00:07:29,750
+الـVmax، this is because at higher concentration، في
+
+115
+00:07:29,750 --> 00:07:34,220
+حال تركيز مرتفع، الـ Active site هيتشبع بيه الـ
+
+116
+00:07:34,220 --> 00:07:37,600
+Substrate لأن الـ Substrate هي اللي هي تركيزها
+
+117
+00:07:37,600 --> 00:07:42,180
+أعلى، أما الـ Inhibitor cannot bind، مش هيرتبط
+
+118
+00:07:42,180 --> 00:07:45,320
+النوع التاني من الـ Inhibitor هو الـ
+
+119
+00:07:45,320 --> 00:07:48,380
+irreversible inhibitor أو الـ non competitive
+
+120
+00:07:48,380 --> 00:07:52,070
+inhibitor، أحكيلي إن هذا الـ Non-competitive مش
+
+121
+00:07:52,070 --> 00:07:57,210
+تنافسي، كيف مش تنافسي؟ إنه إله موقع خاص موجود
+
+122
+00:07:57,210 --> 00:08:00,890
+على الانزيم وبيرتبط فيه، غير الـ Active site، إيش
+
+123
+00:08:00,890 --> 00:08:04,810
+اسم هذا الموقع؟ اسمه الـ Steric site، إن الانزيم
+
+124
+00:08:04,810 --> 00:08:08,030
+بيكون فيه موقع، موقع الـ Active site اللي بيرتبط
+
+125
+00:08:08,030 --> 00:08:11,630
+فيه الـ Substrate، فيه موقع اسمه الـ Steric site
+
+126
+00:08:11,630 --> 00:08:15,470
+بترتبط فيه الـ Non-competitive inhibitor، يعني كل
+
+127
+00:08:15,470 --> 00:08:19,510
+واحد له مكانه، بيجي بيرتبط في هذا المكان اللي نبته
+
+128
+00:08:19,510 --> 00:08:22,810
+في مكانه، و ال substrate في مكانه، فبيحكيلي في هذه
+
+129
+00:08:22,810 --> 00:08:26,770
+الحالة أنت لو مهما زوّتي تركيز ال substrate مش
+
+130
+00:08:26,770 --> 00:08:31,230
+هتحل المشكلة لأن هو مش مابيتنافسوا على نفس الموقع
+
+131
+00:08:31,230 --> 00:08:34,550
+هم كل واحد له موقع، أنا مهما أزيد تركيز ال
+
+132
+00:08:34,550 --> 00:08:38,930
+substrate مش هحل هذه المشكلة، these are not treated
+
+133
+00:08:38,930 --> 00:08:42,030
+by the concentration of substrate، ما بتحل المشكلة
+
+134
+00:08:42,030 --> 00:08:42,330
+بزيادة
diff --git a/PL9fwy3NUQKwZ1Q7zOg1ObgufPMjRI3WY1/OzyPnleVow8_postprocess.srt b/PL9fwy3NUQKwZ1Q7zOg1ObgufPMjRI3WY1/OzyPnleVow8_postprocess.srt
new file mode 100644
index 0000000000000000000000000000000000000000..f7abb6bf5fa827b29af8f1201dfde48c97c29fb8
--- /dev/null
+++ b/PL9fwy3NUQKwZ1Q7zOg1ObgufPMjRI3WY1/OzyPnleVow8_postprocess.srt
@@ -0,0 +1,536 @@
+1
+00:00:00,540 --> 00:00:04,000
+العمل اللي فاد هيك من العوامل اللي بتأثر على نشاط
+
+2
+00:00:04,000 --> 00:00:07,120
+الانزين هو الـInhibitors Inhibitors نسمعناها
+
+3
+00:00:07,120 --> 00:00:10,740
+المطبطان Inhibitors are chemicals that reduce the
+
+4
+00:00:10,740 --> 00:00:16,060
+rate of enzymatic reaction هي مواد بتقلل معدل حدوث
+
+5
+00:00:16,060 --> 00:00:20,140
+التفاعل الكيميائي وبتقلل نشاط الانزين They are
+
+6
+00:00:20,140 --> 00:00:22,880
+usually specific and they work at low
+
+7
+00:00:22,880 --> 00:00:28,890
+concentration هاي الـInhibitorsهي متخصصة،
+
+8
+00:00:28,890 --> 00:00:33,150
+specific، كل inhibitor متخصص لأنزيم معين، يعني في
+
+9
+00:00:33,150 --> 00:00:35,730
+أن الـinhibitor بيخبط الانزيم مثلا على الكلائن
+
+10
+00:00:35,730 --> 00:00:39,170
+الفسفتان، عن ال inhibitor تاني لأنزيم تاني، كل
+
+11
+00:00:39,170 --> 00:00:43,730
+inhibitor بيخبط انزيم معين، يعني متخصص، specific،
+
+12
+00:00:43,730 --> 00:00:46,370
+they block the enzyme but they don't usually
+
+13
+00:00:46,370 --> 00:00:51,290
+destroy it، بيعمل ال inhibitor علىإنه يوقف نشاط
+
+14
+00:00:51,290 --> 00:00:56,470
+الإنزين بدون ما يدمر الإنزين بس بيوقف نشاط الإنزين
+
+15
+00:00:56,470 --> 00:01:02,430
+بدون ما يعمل تدمير للإنزين إيش أنواع الـInhibitors
+
+16
+00:01:02,430 --> 00:01:05,830
+الموجودين؟ في عندنا reversible inhibitor أو
+
+17
+00:01:05,830 --> 00:01:08,870
+irreversible نوعيا، reversible، irreversible
+
+18
+00:01:08,870 --> 00:01:12,430
+الـreversible inhibitor هو نفسه الـcompetitive
+
+19
+00:01:12,430 --> 00:01:17,670
+inhibitor reversible هو competitive inhibitorكيف
+
+20
+00:01:17,670 --> 00:01:21,890
+هدا بيشتغل؟ هدا competitive، تنافسين، بتيجي
+
+21
+00:01:21,890 --> 00:01:26,190
+بتتنافس الـsubstrate والـinhibitor، إنه بيتنافس
+
+22
+00:01:26,190 --> 00:01:30,830
+الـsubstrate والـinhibitor على الإرتباط بالـactive
+
+23
+00:01:30,830 --> 00:01:34,470
+site، فجهة الـsubstrate، الحكينا إنه بيرتبط مع
+
+24
+00:01:34,470 --> 00:01:37,770
+الينزين في موقع اسمه الـactive site، عشان يشتغل،
+
+25
+00:01:38,290 --> 00:01:41,570
+تيجي هاي الـInhibitor بتتنافس مع الـsubstrate على
+
+26
+00:01:41,570 --> 00:01:45,030
+الـactive side، إنه هاي الـInhibitor إليها شكل شبه
+
+27
+00:01:45,030 --> 00:01:48,150
+شكل الـactive side، زيها زي الـsubstrate، فهي
+
+28
+00:01:48,150 --> 00:01:51,310
+بتتنافس هي و الـsubstrate على هذا الموقع، إنه
+
+29
+00:01:51,310 --> 00:01:53,130
+الـInhibitor بيقول لها الـsubstrate أنا بدي
+
+30
+00:01:53,130 --> 00:01:56,570
+أرتبتها، و الـsubstrate بيحكي لأ هذا مكان يعني أنا
+
+31
+00:01:56,570 --> 00:02:00,310
+بدي أرتبتها، هم الإتنين بدهم هذا المكان، طب مين
+
+32
+00:02:00,310 --> 00:02:05,100
+هيفوز؟ بيحكي للي إله أعلى تركيز هو اللي هيفوز،إذا
+
+33
+00:02:05,100 --> 00:02:08,320
+الـ Substrate تركيزها أعلى هي اللي هتفوز وهترتبط
+
+34
+00:02:09,140 --> 00:02:12,580
+اللي له أقل تركيز هو اللي مش هيرتبط إذا كان
+
+35
+00:02:12,580 --> 00:02:16,120
+الـInhibitor تركيزه أعلى، هيرتبط إذا الـSubstrate
+
+36
+00:02:16,120 --> 00:02:19,060
+هي اللي تركيزها أعلى، هي اللي هترتبط طيب، أنا لو
+
+37
+00:02:19,060 --> 00:02:22,420
+عندي مشكلة إنه عندي Competitive Inhibitor موجود
+
+38
+00:02:22,420 --> 00:02:26,640
+كيف بدأ أحل هذه المشكلة عشان أتلاشى و أبعد عن هذا
+
+39
+00:02:26,640 --> 00:02:29,860
+الـInhibitor و أخلي بس الـSubstrate يرتبط؟ سهل،
+
+40
+00:02:29,860 --> 00:02:33,420
+إني أضيف تركيز الـSubstrate بزيانة أضيف على
+
+41
+00:02:33,420 --> 00:02:38,470
+التفاعل Substrate، أضمن أضيف Substrateفبصير تركيز
+
+42
+00:02:38,470 --> 00:02:41,870
+الـ substrate أعلى من الـ inhibitor فهو بيفوز
+
+43
+00:02:41,870 --> 00:02:45,730
+وبيرتبط بدل الـ inhibitor then these compete with
+
+44
+00:02:45,730 --> 00:02:48,910
+the substrate molecule for active site بيتنافس مع
+
+45
+00:02:48,910 --> 00:02:52,530
+ال substrate علاش على الactive siteالـ Inhibitor
+
+46
+00:02:52,530 --> 00:02:55,730
+actually proportional to its concentration إن
+
+47
+00:02:55,730 --> 00:03:00,570
+تركيز الـ Inhibitor بيعتمد ما عمله إنه كل ما زاد
+
+48
+00:03:00,570 --> 00:03:04,230
+تركيز الـ Inhibitor كل ما زاد ارتباطه بسبب الـ
+
+49
+00:03:04,230 --> 00:03:07,990
+enzyme في الـ active site وهو اللي هيفوز لكن كل ما
+
+50
+00:03:07,990 --> 00:03:12,730
+قل تركيز الـ Inhibitor بيقل الارتباط الـ inhibitor
+
+51
+00:03:12,730 --> 00:03:17,040
+مع الـ enzymecome over this problem by adding more
+
+52
+00:03:17,040 --> 00:03:20,980
+substrate كيف بدي أحل المشكلة دي و أتلافق مشكلة
+
+53
+00:03:20,980 --> 00:03:24,920
+وجود competitive inhibitor أني أضيف substrate بحيث
+
+54
+00:03:24,920 --> 00:03:28,840
+أن يكون تركيز ال substrate أعلى من تركيز ال
+
+55
+00:03:28,840 --> 00:03:33,250
+inhibitorطب في عنا مخطط على الجانب بيوضحلي إنه كيف
+
+56
+00:03:33,250 --> 00:03:36,730
+هيمشي التفاعل في وجود «no inhibitor» وفي حال وجود
+
+57
+00:03:36,730 --> 00:03:40,510
+«competitive inhibitor» هلجيت في الحالتين سواء في
+
+58
+00:03:40,510 --> 00:03:45,130
+inhibitor أو فيش inhibitor أنا هاصل لأقصى سرعة من
+
+59
+00:03:45,130 --> 00:03:49,750
+التفاعل الكيميائي ولكن هل هامشي بنفس السرعة ولا لأ
+
+60
+00:03:49,750 --> 00:03:53,010
+في الحالتين في وجود inhibitor أو في عدم وجود
+
+61
+00:03:53,010 --> 00:03:56,830
+inhibitor أنا هاصل الـVmax هاصل لأقصى سرعة من
+
+62
+00:03:56,830 --> 00:04:00,460
+التفاعللكن الحين لو فيش الـInhibitor، هيمش التفاعل
+
+63
+00:04:00,460 --> 00:04:03,820
+عادي، هيوصل لأقصى سرعة من التفاعل، عند أقصى
+
+64
+00:04:03,820 --> 00:04:07,220
+السرعة، بتتشبع كل الانزين بالـSubstrate، بيصير عنا
+
+65
+00:04:07,220 --> 00:04:11,440
+Saturation Point ونقطة التشبع، فهيثبت الملحنة، هدا
+
+66
+00:04:11,440 --> 00:04:15,320
+في عدم وجود Inhibitor، أنا أوصل فيه Max، تمام؟هذا
+
+67
+00:04:15,320 --> 00:04:18,560
+حكينا عنه قبلها، بيبقى عدم وجود Inhibitor حكينا
+
+68
+00:04:18,560 --> 00:04:21,600
+كيف بيشتغل الانزيم إنه كل ما زاد تركيز الـ
+
+69
+00:04:21,600 --> 00:04:25,740
+Substrate بتزيد سرعة التفاعل وصولا إلى الـ
+
+70
+00:04:25,740 --> 00:04:29,320
+saturation point، إلى نقطة التشابع، يعني نقطة
+
+71
+00:04:29,320 --> 00:04:33,160
+التشابع بيثبت المنحنة لأنه خلاص كل الانزيمات
+
+72
+00:04:33,160 --> 00:04:36,440
+متشبعة في الـ Substrate ففيش زيادة لسرعة التفاعل
+
+73
+00:04:36,440 --> 00:04:40,420
+تقنيجي لو عدم وجود Competitive Inhibitor في حال
+
+74
+00:04:40,420 --> 00:04:44,690
+وجود، وجود Competitive Inhibitorفي حال وجود الـ
+
+75
+00:04:44,690 --> 00:04:47,970
+Competitive Inhibitor حكانة إنه أنا بدي أوصل
+
+76
+00:04:47,970 --> 00:04:51,730
+لـVmax لأقصى سرعة من التفاعل لكن كيف بدي أشيل هذا
+
+77
+00:04:51,730 --> 00:04:55,910
+الـInhibitor؟ بدي أزود الـSubstrate مين اللي بيعبر
+
+78
+00:04:55,910 --> 00:04:58,750
+عن تركيز الـSubstrate حكانة اللي هي عبارة عن تركيز
+
+79
+00:04:58,750 --> 00:05:03,240
+الـSubstrate؟ هي KMمش حاكينا «KM» هي تركيز الـ
+
+80
+00:05:03,240 --> 00:05:07,180
+«Substrate» عن نُص «Vmax» فهي بتعبّر عن تركيز الـ
+
+81
+00:05:07,180 --> 00:05:11,100
+«Substrate» فأنا مين هزود؟ هل هلعب في «Vmax»؟ هزود
+
+82
+00:05:11,100 --> 00:05:14,520
+«Vmax»؟ لأ، أنا بدي أزود تركيز الـ «Substrate»
+
+83
+00:05:14,520 --> 00:05:19,100
+فبدي أزود «KM»، إذا في وجود الـ «Inhibitor» «KM»
+
+84
+00:05:19,100 --> 00:05:24,380
+هتتغير، هتزيد، إيش هيصير عليها؟ هتزيد «KM»في وجود
+
+85
+00:05:24,380 --> 00:05:28,560
+الـ «Inhibitor» هزيد «Km» «Km» increase لكن «Vmax»
+
+86
+00:05:28,560 --> 00:05:33,440
+هل هتتأثر؟ لأ، هوصل لـ «Vmax» فـ «Vmax» نفسها ما
+
+87
+00:05:33,440 --> 00:05:38,760
+هتتغير مش هتتغير وإنما مين اللي هيتغير بس «Km»
+
+88
+00:05:38,760 --> 00:05:44,790
+تركيز الـ «substrate»هنا إيش مكتوب؟ KM increase
+
+89
+00:05:44,790 --> 00:05:48,110
+بتزيد KM طب ليش؟ لأن addition of competitive
+
+90
+00:05:48,110 --> 00:05:52,870
+inhibitor increased KM for a given substrate في
+
+91
+00:05:52,870 --> 00:05:56,870
+حال وجود competitive inhibitor بتزيد KM عشان أزود
+
+92
+00:05:56,870 --> 00:06:00,610
+تركيز الـsubstrate عشان الـsubstrate يفوز ويكون
+
+93
+00:06:00,610 --> 00:06:04,130
+تركيزه أعلى من الـcompetitive inhibitor وهو اللي
+
+94
+00:06:04,130 --> 00:06:09,200
+يفوز في موقع الـactive site في الإرتباطفي حالة الـ
+
+95
+00:06:09,200 --> 00:06:11,280
+Competitive Inhibitor يحتاج المزيد من الـ
+
+96
+00:06:11,280 --> 00:06:15,520
+Substrate لإيقافها لوجود Competitive Inhibitor أنا
+
+97
+00:06:15,520 --> 00:06:18,500
+بحتاج إلى مزيد من الـ Substrate عشان يرتبط مع
+
+98
+00:06:18,500 --> 00:06:22,820
+النظيم و هو اللي يفوز و يمشي التفاعل و يوصل فيما
+
+99
+00:06:24,530 --> 00:06:28,810
+أما Vmax ما تتغير سرعة التفاعل أقصى سرعة التفاعل
+
+100
+00:06:28,810 --> 00:06:32,670
+مش هتتغير هي هي في حال وجود inhibitor أو في عدم
+
+101
+00:06:32,670 --> 00:06:36,510
+وجود inhibitor don't competitive inhibitor don't
+
+102
+00:06:36,510 --> 00:06:40,610
+alter the Vmax مابيغيرش Vmax effect of competitive
+
+103
+00:06:40,610 --> 00:06:43,970
+inhibitor is reversed by increasing substrate
+
+104
+00:06:43,970 --> 00:06:47,590
+concentration high substrate concentration the
+
+105
+00:06:47,590 --> 00:06:51,870
+reaction velocity reached the same Vmax as that
+
+106
+00:06:51,870 --> 00:06:57,340
+observed in the absence of the inhibitorمحكي تأثير
+
+107
+00:06:57,340 --> 00:07:01,180
+الـ Competitive Inhibitor بقدر إني أتلاشى وإني
+
+108
+00:07:01,180 --> 00:07:05,440
+أشيلهم من خلال زيادة تركيز الـSubstrate إذا زودت
+
+109
+00:07:05,440 --> 00:07:09,540
+تركيز الـSubstrate، التفاعل بيوصل لـVmax نفس اللي
+
+110
+00:07:09,540 --> 00:07:14,190
+كانت في الأول، بيوصل لأقصى سرعة من التفاعلفي اللي
+
+111
+00:07:14,190 --> 00:07:17,550
+هي نفس السرعة في حال غياب الـInhibitor هاصل
+
+112
+00:07:17,550 --> 00:07:21,430
+الـVmax سواء فيه Inhibitor ولا فاشر بس في حال وجود
+
+113
+00:07:21,430 --> 00:07:25,110
+Inhibitor لازم أزود تركيز الـSubstrate عشان أحصل
+
+114
+00:07:25,110 --> 00:07:29,750
+الـVmax this is because at higher concentration في
+
+115
+00:07:29,750 --> 00:07:34,220
+حال تركيز مرتفعالـ Active site هيتشبع بيه الـ
+
+116
+00:07:34,220 --> 00:07:37,600
+Substrate لأن الـ Substrate هي اللي هي التركيزها
+
+117
+00:07:37,600 --> 00:07:42,180
+أعلى أما الـ Inhibitor cannot bind مش هيرتبط
+
+118
+00:07:42,180 --> 00:07:45,320
+الانتباه التاني من الـ Inhibitor هو الـ
+
+119
+00:07:45,320 --> 00:07:48,380
+irreversible inhibitor أو الـ non competitive
+
+120
+00:07:48,380 --> 00:07:52,070
+inhibitorأحكيلي إن هذا الـ Non-competitive مش
+
+121
+00:07:52,070 --> 00:07:57,210
+تنافسي، كيف مش تنافسي؟ إنه BGN إله موقع خاص موجود
+
+122
+00:07:57,210 --> 00:08:00,890
+على الإنزيم وبيرتبط فيه، غير الـ Active site إيش
+
+123
+00:08:00,890 --> 00:08:04,810
+اسم هذا الموقع؟ اسمه الـ Steric site إن الإنزيم
+
+124
+00:08:04,810 --> 00:08:08,030
+بيكون فيه موقع، موقع الـ Active site اللي بيرتبط
+
+125
+00:08:08,030 --> 00:08:11,630
+فيه الـ Substrate فيه موقع اسمه الـ Steric site
+
+126
+00:08:11,630 --> 00:08:15,470
+بترتبط فيه الـ Non-competitive inhibitorيعني كل
+
+127
+00:08:15,470 --> 00:08:19,510
+واحد له مكانه بيجي بيرطبط في هذا المكان اللي نبته
+
+128
+00:08:19,510 --> 00:08:22,810
+في مكانه و ال substrate في مكانه فبيحكيلي في هذه
+
+129
+00:08:22,810 --> 00:08:26,770
+الحالة أنت لو مهما زوّتي تركيز ال substrate مش
+
+130
+00:08:26,770 --> 00:08:31,230
+هتحل المشكلة لأن هو مش مابيتنافسوا على نفس الموقع
+
+131
+00:08:31,230 --> 00:08:34,550
+هم كل واحد له موقع أنا مهما أزيد تركيز ال
+
+132
+00:08:34,550 --> 00:08:38,930
+substrate مش هحل هذه المشكلة these are not treated
+
+133
+00:08:38,930 --> 00:08:42,030
+by the concentration of substrate ما بتحل المشكلة
+
+134
+00:08:42,030 --> 00:08:42,330
+بزيادة
+
diff --git a/PL9fwy3NUQKwZ1Q7zOg1ObgufPMjRI3WY1/OzyPnleVow8_raw.json b/PL9fwy3NUQKwZ1Q7zOg1ObgufPMjRI3WY1/OzyPnleVow8_raw.json
new file mode 100644
index 0000000000000000000000000000000000000000..6706db8e1f0768fc6991d9b381bfb541179accd2
--- /dev/null
+++ b/PL9fwy3NUQKwZ1Q7zOg1ObgufPMjRI3WY1/OzyPnleVow8_raw.json
@@ -0,0 +1 @@
+{"segments": [{"id": 1, "seek": 2658, "start": 0.54, "end": 26.58, "text": "العمل اللي فاد هيك من العوامل اللي بتأثر على نشاط الانزين هو الـInhibitors Inhibitors نسمعناها المطبطان Inhibitors are chemicals that reduce the rate of enzymatic reaction هي مواد بتقلل معدل حدوث التفاعل الكيميائي وبتقلل نشاط الانزين They are usually specific and they work at low concentration هاي الـInhibitors", "tokens": [6027, 25957, 1211, 13672, 1829, 6156, 18513, 39896, 4117, 9154, 18863, 2407, 10943, 1211, 13672, 1829, 39894, 10721, 49115, 15844, 8717, 8592, 41193, 2423, 7649, 11622, 9957, 31439, 2423, 39184, 4575, 5455, 9862, 682, 5455, 9862, 8717, 38251, 3615, 8315, 11296, 9673, 9566, 3555, 9566, 7649, 682, 5455, 9862, 366, 16152, 300, 5407, 264, 3314, 295, 16272, 25915, 5480, 39896, 3714, 14407, 3215, 39894, 4587, 1211, 1211, 20449, 3215, 1211, 11331, 3215, 2407, 12984, 16712, 5172, 995, 30241, 33251, 32640, 1829, 16373, 1829, 46599, 2655, 4587, 1211, 1211, 8717, 8592, 41193, 2423, 7649, 11622, 9957, 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{"start": 328.7, "end": 330.0, "word": " هتتأثر؟", "probability": 0.8534342447916666}, {"start": 330.0, "end": 330.44, "word": " لأ،", "probability": 0.8849283854166666}, {"start": 330.44, "end": 331.04, "word": " هوصل", "probability": 0.8994140625}, {"start": 331.04, "end": 331.28, "word": " لـ", "probability": 0.762451171875}, {"start": 331.28, "end": 331.86, "word": " «Vmax»", "probability": 0.9317626953125}, {"start": 331.86, "end": 332.0, "word": " فـ", "probability": 0.717041015625}, {"start": 332.0, "end": 332.7, "word": " «Vmax»", "probability": 0.9019775390625}, {"start": 332.7, "end": 333.24, "word": " نفسها", "probability": 0.9957682291666666}, {"start": 333.24, "end": 333.44, "word": " ما", "probability": 0.3115234375}, {"start": 333.44, "end": 333.94, "word": " هتتغير", "probability": 0.874609375}, {"start": 333.94, "end": 334.1, "word": " مش", "probability": 0.55615234375}, {"start": 334.1, "end": 334.7, "word": " هتتغير", "probability": 0.99599609375}, {"start": 334.7, 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سواء فيه Inhibitor ولا فاشر بس في حال وجود Inhibitor لازم أزود تركيز الـSubstrate عشان أحصل الـVmax this is because at higher concentration في حال تركيز مرتفع", "tokens": [41185, 13672, 1829, 39896, 8717, 36178, 21136, 2288, 27884, 8978, 11331, 6027, 32771, 1829, 16758, 2423, 39184, 4575, 5455, 3029, 8032, 33546, 1211, 2423, 39184, 53, 41167, 8608, 2407, 16606, 8978, 3224, 682, 5455, 3029, 49429, 6156, 33599, 2288, 4724, 3794, 8978, 11331, 6027, 49610, 23328, 682, 5455, 3029, 5296, 31377, 2304, 5551, 11622, 23328, 6055, 31747, 1829, 11622, 2423, 39184, 39582, 372, 4404, 6225, 8592, 7649, 5551, 5016, 36520, 2423, 39184, 53, 41167, 341, 307, 570, 412, 2946, 9856, 8978, 11331, 6027, 6055, 31747, 1829, 11622, 3714, 43500, 5172, 3615], "avg_logprob": -0.168138586146676, "compression_ratio": 1.5970149253731343, "no_speech_prob": 3.355741500854492e-05, "words": [{"start": 433.45, "end": 433.75, "word": "في", "probability": 0.73583984375}, {"start": 433.75, "end": 434.19, "word": " اللي", 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إن هذا الـ Non-competitive مش تنافسي، كيف مش تنافسي؟ إنه BGN إله موقع خاص موجود على الإنزيم وبيرتبط فيه، غير الـ Active site إيش اسم هذا الموقع؟ اسمه الـ Steric site إن الإنزيم بيكون فيه موقع، موقع الـ Active site اللي بيرتبط فيه الـ Substrate فيه موقع اسمه الـ Steric site بترتبط فيه الـ Non-competitive inhibitor", "tokens": [10721, 5016, 4117, 1829, 20292, 36145, 23758, 2423, 39184, 8774, 12, 1112, 7275, 2187, 37893, 6055, 8315, 36178, 1829, 12399, 9122, 33911, 37893, 6055, 8315, 36178, 1829, 22807, 36145, 3224, 363, 38, 45, 11933, 43761, 3714, 30543, 3615, 16490, 33546, 3714, 29245, 23328, 15844, 33688, 1863, 11622, 32640, 4032, 21292, 43500, 3555, 9566, 8978, 3224, 12399, 32771, 13546, 2423, 39184, 26635, 3621, 11933, 1829, 8592, 24525, 2304, 23758, 9673, 30543, 3615, 22807, 24525, 2304, 3224, 2423, 39184, 33539, 299, 3621, 36145, 33688, 1863, 11622, 32640, 4724, 1829, 30544, 8978, 3224, 3714, 30543, 3615, 12399, 3714, 30543, 3615, 2423, 39184, 26635, 3621, 13672, 1829, 4724, 13546, 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" Non", "probability": 0.94189453125}, {"start": 492.99, "end": 493.67, "word": "-competitive", "probability": 0.94384765625}, {"start": 493.67, "end": 494.25, "word": " inhibitor", "probability": 0.898193359375}], "temperature": 1.0}, {"id": 24, "seek": 52233, "start": 494.99, "end": 522.33, "text": "يعني كل واحد له مكانه بيجي بيرطبط في هذا المكان اللي نبته في مكانه و ال substrate في مكانه فبيحكيلي في هذه الحالة أنت لو مهما زوّتي تركيز ال substrate مش هتحل المشكلة لأن هو مش مابيتنافسوا على نفس الموقع هم كل واحد له موقع أنا مهما أزيد تركيز ال substrate مش هحل هذه المشكلة these are not treated by the concentration of substrate ما بتحل المشكلة بزيادة", "tokens": [40228, 22653, 28242, 36764, 24401, 46740, 3714, 41361, 3224, 4724, 1829, 7435, 1829, 4724, 13546, 9566, 3555, 9566, 8978, 23758, 9673, 41361, 13672, 1829, 8717, 3555, 2655, 3224, 8978, 3714, 41361, 3224, 4032, 2423, 27585, 8978, 3714, 41361, 3224, 6156, 21292, 5016, 4117, 1829, 20292, 8978, 29538, 21542, 6027, 3660, 14739, 2655, 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له", "probability": 0.447509765625}, {"start": 496.01, "end": 496.61, "word": " مكانه", "probability": 0.990234375}, {"start": 496.61, "end": 497.11, "word": " بيجي", "probability": 0.74365234375}, {"start": 497.11, "end": 497.63, "word": " بيرطبط", "probability": 0.80009765625}, {"start": 497.63, "end": 497.77, "word": " في", "probability": 0.91845703125}, {"start": 497.77, "end": 498.19, "word": " هذا", "probability": 0.927734375}, {"start": 498.19, "end": 498.69, "word": " المكان", "probability": 0.982666015625}, {"start": 498.69, "end": 499.09, "word": " اللي", "probability": 0.8076171875}, {"start": 499.09, "end": 499.51, "word": " نبته", "probability": 0.45257568359375}, {"start": 499.51, "end": 499.67, "word": " في", "probability": 0.95654296875}, {"start": 499.67, "end": 500.09, "word": " مكانه", "probability": 0.9856770833333334}, {"start": 500.09, "end": 500.21, "word": " و", "probability": 0.63818359375}, {"start": 500.21, "end": 500.33, "word": " ال", "probability": 0.880859375}, {"start": 500.33, "end": 500.61, "word": " substrate", "probability": 0.414794921875}, {"start": 500.61, "end": 500.87, "word": " في", "probability": 0.9609375}, {"start": 500.87, "end": 501.33, "word": " مكانه", "probability": 0.9949544270833334}, {"start": 501.33, "end": 502.39, "word": " فبيحكيلي", "probability": 0.8807779947916666}, {"start": 502.39, "end": 502.53, "word": " في", "probability": 0.947265625}, {"start": 502.53, "end": 502.81, "word": " هذه", "probability": 0.84423828125}, {"start": 502.81, "end": 503.45, "word": " الحالة", "probability": 0.9943033854166666}, {"start": 503.45, "end": 503.99, "word": " أنت", "probability": 0.6046142578125}, {"start": 503.99, "end": 504.19, "word": " لو", "probability": 0.86572265625}, {"start": 504.19, "end": 504.59, "word": " مهما", "probability": 0.9091796875}, {"start": 504.59, "end": 505.11, "word": " زوّتي", "probability": 0.6619873046875}, {"start": 505.11, "end": 505.55, "word": " تركيز", "probability": 0.9906005859375}, {"start": 505.55, "end": 505.69, "word": " ال", "probability": 0.96044921875}, {"start": 505.69, "end": 506.19, "word": " substrate", "probability": 0.90283203125}, {"start": 506.19, "end": 506.77, "word": " مش", "probability": 0.828125}, {"start": 506.77, "end": 507.13, "word": " هتحل", "probability": 0.9906005859375}, {"start": 507.13, "end": 507.93, "word": " المشكلة", "probability": 0.98974609375}, {"start": 507.93, "end": 508.37, "word": " لأن", "probability": 0.78125}, {"start": 508.37, "end": 508.59, "word": " هو", "probability": 0.84326171875}, {"start": 508.59, "end": 508.85, "word": " مش", "probability": 0.60498046875}, {"start": 508.85, "end": 510.39, "word": " مابيتنافسوا", "probability": 0.8585611979166666}, {"start": 510.39, "end": 510.53, "word": " على", "probability": 0.90771484375}, {"start": 510.53, "end": 510.79, "word": " نفس", "probability": 0.99853515625}, {"start": 510.79, "end": 511.23, "word": " الموقع", "probability": 0.9881184895833334}, {"start": 511.23, "end": 511.43, "word": " هم", "probability": 0.921875}, {"start": 511.43, "end": 511.65, "word": " كل", "probability": 0.9833984375}, {"start": 511.65, "end": 512.03, "word": " واحد", "probability": 0.99609375}, {"start": 512.03, "end": 512.23, "word": " له", "probability": 0.9423828125}, {"start": 512.23, "end": 512.71, "word": " موقع", "probability": 0.9895833333333334}, {"start": 512.71, "end": 513.23, "word": " أنا", "probability": 0.74609375}, {"start": 513.23, "end": 513.63, "word": " مهما", "probability": 0.9431966145833334}, {"start": 513.63, "end": 513.95, "word": " أزيد", "probability": 0.9474283854166666}, {"start": 513.95, "end": 514.41, "word": " تركيز", "probability": 0.996337890625}, {"start": 514.41, "end": 514.55, "word": " ال", "probability": 0.982421875}, {"start": 514.55, "end": 514.95, "word": " substrate", "probability": 0.96044921875}, {"start": 514.95, "end": 515.25, "word": " مش", "probability": 0.97802734375}, {"start": 515.25, "end": 515.55, "word": " هحل", "probability": 0.9938151041666666}, {"start": 515.55, "end": 515.83, "word": " هذه", "probability": 0.89111328125}, {"start": 515.83, "end": 516.47, "word": " المشكلة", "probability": 0.9971923828125}, {"start": 516.47, "end": 518.05, "word": " these", "probability": 0.4560546875}, {"start": 518.05, "end": 518.35, "word": " are", "probability": 0.9560546875}, {"start": 518.35, "end": 518.59, "word": " not", "probability": 0.95751953125}, {"start": 518.59, "end": 518.93, "word": " treated", "probability": 0.7861328125}, {"start": 518.93, "end": 519.17, "word": " by", "probability": 0.94873046875}, {"start": 519.17, "end": 519.35, "word": " the", "probability": 0.86474609375}, {"start": 519.35, "end": 519.93, "word": " concentration", "probability": 0.92041015625}, {"start": 519.93, "end": 520.17, "word": " of", "probability": 0.97802734375}, {"start": 520.17, "end": 520.83, "word": " substrate", "probability": 0.93017578125}, {"start": 520.83, "end": 521.09, "word": " ما", "probability": 0.5849609375}, {"start": 521.09, "end": 521.59, "word": " بتحل", "probability": 0.8868815104166666}, {"start": 521.59, "end": 522.03, "word": " المشكلة", "probability": 0.994873046875}, {"start": 522.03, "end": 522.33, "word": " بزيادة", "probability": 0.97802734375}], "temperature": 1.0}], "language": "ar", "language_probability": 1.0, "duration": 522.332875, "duration_after_vad": 516.9600000000005}
\ No newline at end of file
diff --git a/PL9fwy3NUQKwZ1Q7zOg1ObgufPMjRI3WY1/OzyPnleVow8_raw.srt b/PL9fwy3NUQKwZ1Q7zOg1ObgufPMjRI3WY1/OzyPnleVow8_raw.srt
new file mode 100644
index 0000000000000000000000000000000000000000..f7abb6bf5fa827b29af8f1201dfde48c97c29fb8
--- /dev/null
+++ b/PL9fwy3NUQKwZ1Q7zOg1ObgufPMjRI3WY1/OzyPnleVow8_raw.srt
@@ -0,0 +1,536 @@
+1
+00:00:00,540 --> 00:00:04,000
+العمل اللي فاد هيك من العوامل اللي بتأثر على نشاط
+
+2
+00:00:04,000 --> 00:00:07,120
+الانزين هو الـInhibitors Inhibitors نسمعناها
+
+3
+00:00:07,120 --> 00:00:10,740
+المطبطان Inhibitors are chemicals that reduce the
+
+4
+00:00:10,740 --> 00:00:16,060
+rate of enzymatic reaction هي مواد بتقلل معدل حدوث
+
+5
+00:00:16,060 --> 00:00:20,140
+التفاعل الكيميائي وبتقلل نشاط الانزين They are
+
+6
+00:00:20,140 --> 00:00:22,880
+usually specific and they work at low
+
+7
+00:00:22,880 --> 00:00:28,890
+concentration هاي الـInhibitorsهي متخصصة،
+
+8
+00:00:28,890 --> 00:00:33,150
+specific، كل inhibitor متخصص لأنزيم معين، يعني في
+
+9
+00:00:33,150 --> 00:00:35,730
+أن الـinhibitor بيخبط الانزيم مثلا على الكلائن
+
+10
+00:00:35,730 --> 00:00:39,170
+الفسفتان، عن ال inhibitor تاني لأنزيم تاني، كل
+
+11
+00:00:39,170 --> 00:00:43,730
+inhibitor بيخبط انزيم معين، يعني متخصص، specific،
+
+12
+00:00:43,730 --> 00:00:46,370
+they block the enzyme but they don't usually
+
+13
+00:00:46,370 --> 00:00:51,290
+destroy it، بيعمل ال inhibitor علىإنه يوقف نشاط
+
+14
+00:00:51,290 --> 00:00:56,470
+الإنزين بدون ما يدمر الإنزين بس بيوقف نشاط الإنزين
+
+15
+00:00:56,470 --> 00:01:02,430
+بدون ما يعمل تدمير للإنزين إيش أنواع الـInhibitors
+
+16
+00:01:02,430 --> 00:01:05,830
+الموجودين؟ في عندنا reversible inhibitor أو
+
+17
+00:01:05,830 --> 00:01:08,870
+irreversible نوعيا، reversible، irreversible
+
+18
+00:01:08,870 --> 00:01:12,430
+الـreversible inhibitor هو نفسه الـcompetitive
+
+19
+00:01:12,430 --> 00:01:17,670
+inhibitor reversible هو competitive inhibitorكيف
+
+20
+00:01:17,670 --> 00:01:21,890
+هدا بيشتغل؟ هدا competitive، تنافسين، بتيجي
+
+21
+00:01:21,890 --> 00:01:26,190
+بتتنافس الـsubstrate والـinhibitor، إنه بيتنافس
+
+22
+00:01:26,190 --> 00:01:30,830
+الـsubstrate والـinhibitor على الإرتباط بالـactive
+
+23
+00:01:30,830 --> 00:01:34,470
+site، فجهة الـsubstrate، الحكينا إنه بيرتبط مع
+
+24
+00:01:34,470 --> 00:01:37,770
+الينزين في موقع اسمه الـactive site، عشان يشتغل،
+
+25
+00:01:38,290 --> 00:01:41,570
+تيجي هاي الـInhibitor بتتنافس مع الـsubstrate على
+
+26
+00:01:41,570 --> 00:01:45,030
+الـactive side، إنه هاي الـInhibitor إليها شكل شبه
+
+27
+00:01:45,030 --> 00:01:48,150
+شكل الـactive side، زيها زي الـsubstrate، فهي
+
+28
+00:01:48,150 --> 00:01:51,310
+بتتنافس هي و الـsubstrate على هذا الموقع، إنه
+
+29
+00:01:51,310 --> 00:01:53,130
+الـInhibitor بيقول لها الـsubstrate أنا بدي
+
+30
+00:01:53,130 --> 00:01:56,570
+أرتبتها، و الـsubstrate بيحكي لأ هذا مكان يعني أنا
+
+31
+00:01:56,570 --> 00:02:00,310
+بدي أرتبتها، هم الإتنين بدهم هذا المكان، طب مين
+
+32
+00:02:00,310 --> 00:02:05,100
+هيفوز؟ بيحكي للي إله أعلى تركيز هو اللي هيفوز،إذا
+
+33
+00:02:05,100 --> 00:02:08,320
+الـ Substrate تركيزها أعلى هي اللي هتفوز وهترتبط
+
+34
+00:02:09,140 --> 00:02:12,580
+اللي له أقل تركيز هو اللي مش هيرتبط إذا كان
+
+35
+00:02:12,580 --> 00:02:16,120
+الـInhibitor تركيزه أعلى، هيرتبط إذا الـSubstrate
+
+36
+00:02:16,120 --> 00:02:19,060
+هي اللي تركيزها أعلى، هي اللي هترتبط طيب، أنا لو
+
+37
+00:02:19,060 --> 00:02:22,420
+عندي مشكلة إنه عندي Competitive Inhibitor موجود
+
+38
+00:02:22,420 --> 00:02:26,640
+كيف بدأ أحل هذه المشكلة عشان أتلاشى و أبعد عن هذا
+
+39
+00:02:26,640 --> 00:02:29,860
+الـInhibitor و أخلي بس الـSubstrate يرتبط؟ سهل،
+
+40
+00:02:29,860 --> 00:02:33,420
+إني أضيف تركيز الـSubstrate بزيانة أضيف على
+
+41
+00:02:33,420 --> 00:02:38,470
+التفاعل Substrate، أضمن أضيف Substrateفبصير تركيز
+
+42
+00:02:38,470 --> 00:02:41,870
+الـ substrate أعلى من الـ inhibitor فهو بيفوز
+
+43
+00:02:41,870 --> 00:02:45,730
+وبيرتبط بدل الـ inhibitor then these compete with
+
+44
+00:02:45,730 --> 00:02:48,910
+the substrate molecule for active site بيتنافس مع
+
+45
+00:02:48,910 --> 00:02:52,530
+ال substrate علاش على الactive siteالـ Inhibitor
+
+46
+00:02:52,530 --> 00:02:55,730
+actually proportional to its concentration إن
+
+47
+00:02:55,730 --> 00:03:00,570
+تركيز الـ Inhibitor بيعتمد ما عمله إنه كل ما زاد
+
+48
+00:03:00,570 --> 00:03:04,230
+تركيز الـ Inhibitor كل ما زاد ارتباطه بسبب الـ
+
+49
+00:03:04,230 --> 00:03:07,990
+enzyme في الـ active site وهو اللي هيفوز لكن كل ما
+
+50
+00:03:07,990 --> 00:03:12,730
+قل تركيز الـ Inhibitor بيقل الارتباط الـ inhibitor
+
+51
+00:03:12,730 --> 00:03:17,040
+مع الـ enzymecome over this problem by adding more
+
+52
+00:03:17,040 --> 00:03:20,980
+substrate كيف بدي أحل المشكلة دي و أتلافق مشكلة
+
+53
+00:03:20,980 --> 00:03:24,920
+وجود competitive inhibitor أني أضيف substrate بحيث
+
+54
+00:03:24,920 --> 00:03:28,840
+أن يكون تركيز ال substrate أعلى من تركيز ال
+
+55
+00:03:28,840 --> 00:03:33,250
+inhibitorطب في عنا مخطط على الجانب بيوضحلي إنه كيف
+
+56
+00:03:33,250 --> 00:03:36,730
+هيمشي التفاعل في وجود «no inhibitor» وفي حال وجود
+
+57
+00:03:36,730 --> 00:03:40,510
+«competitive inhibitor» هلجيت في الحالتين سواء في
+
+58
+00:03:40,510 --> 00:03:45,130
+inhibitor أو فيش inhibitor أنا هاصل لأقصى سرعة من
+
+59
+00:03:45,130 --> 00:03:49,750
+التفاعل الكيميائي ولكن هل هامشي بنفس السرعة ولا لأ
+
+60
+00:03:49,750 --> 00:03:53,010
+في الحالتين في وجود inhibitor أو في عدم وجود
+
+61
+00:03:53,010 --> 00:03:56,830
+inhibitor أنا هاصل الـVmax هاصل لأقصى سرعة من
+
+62
+00:03:56,830 --> 00:04:00,460
+التفاعللكن الحين لو فيش الـInhibitor، هيمش التفاعل
+
+63
+00:04:00,460 --> 00:04:03,820
+عادي، هيوصل لأقصى سرعة من التفاعل، عند أقصى
+
+64
+00:04:03,820 --> 00:04:07,220
+السرعة، بتتشبع كل الانزين بالـSubstrate، بيصير عنا
+
+65
+00:04:07,220 --> 00:04:11,440
+Saturation Point ونقطة التشبع، فهيثبت الملحنة، هدا
+
+66
+00:04:11,440 --> 00:04:15,320
+في عدم وجود Inhibitor، أنا أوصل فيه Max، تمام؟هذا
+
+67
+00:04:15,320 --> 00:04:18,560
+حكينا عنه قبلها، بيبقى عدم وجود Inhibitor حكينا
+
+68
+00:04:18,560 --> 00:04:21,600
+كيف بيشتغل الانزيم إنه كل ما زاد تركيز الـ
+
+69
+00:04:21,600 --> 00:04:25,740
+Substrate بتزيد سرعة التفاعل وصولا إلى الـ
+
+70
+00:04:25,740 --> 00:04:29,320
+saturation point، إلى نقطة التشابع، يعني نقطة
+
+71
+00:04:29,320 --> 00:04:33,160
+التشابع بيثبت المنحنة لأنه خلاص كل الانزيمات
+
+72
+00:04:33,160 --> 00:04:36,440
+متشبعة في الـ Substrate ففيش زيادة لسرعة التفاعل
+
+73
+00:04:36,440 --> 00:04:40,420
+تقنيجي لو عدم وجود Competitive Inhibitor في حال
+
+74
+00:04:40,420 --> 00:04:44,690
+وجود، وجود Competitive Inhibitorفي حال وجود الـ
+
+75
+00:04:44,690 --> 00:04:47,970
+Competitive Inhibitor حكانة إنه أنا بدي أوصل
+
+76
+00:04:47,970 --> 00:04:51,730
+لـVmax لأقصى سرعة من التفاعل لكن كيف بدي أشيل هذا
+
+77
+00:04:51,730 --> 00:04:55,910
+الـInhibitor؟ بدي أزود الـSubstrate مين اللي بيعبر
+
+78
+00:04:55,910 --> 00:04:58,750
+عن تركيز الـSubstrate حكانة اللي هي عبارة عن تركيز
+
+79
+00:04:58,750 --> 00:05:03,240
+الـSubstrate؟ هي KMمش حاكينا «KM» هي تركيز الـ
+
+80
+00:05:03,240 --> 00:05:07,180
+«Substrate» عن نُص «Vmax» فهي بتعبّر عن تركيز الـ
+
+81
+00:05:07,180 --> 00:05:11,100
+«Substrate» فأنا مين هزود؟ هل هلعب في «Vmax»؟ هزود
+
+82
+00:05:11,100 --> 00:05:14,520
+«Vmax»؟ لأ، أنا بدي أزود تركيز الـ «Substrate»
+
+83
+00:05:14,520 --> 00:05:19,100
+فبدي أزود «KM»، إذا في وجود الـ «Inhibitor» «KM»
+
+84
+00:05:19,100 --> 00:05:24,380
+هتتغير، هتزيد، إيش هيصير عليها؟ هتزيد «KM»في وجود
+
+85
+00:05:24,380 --> 00:05:28,560
+الـ «Inhibitor» هزيد «Km» «Km» increase لكن «Vmax»
+
+86
+00:05:28,560 --> 00:05:33,440
+هل هتتأثر؟ لأ، هوصل لـ «Vmax» فـ «Vmax» نفسها ما
+
+87
+00:05:33,440 --> 00:05:38,760
+هتتغير مش هتتغير وإنما مين اللي هيتغير بس «Km»
+
+88
+00:05:38,760 --> 00:05:44,790
+تركيز الـ «substrate»هنا إيش مكتوب؟ KM increase
+
+89
+00:05:44,790 --> 00:05:48,110
+بتزيد KM طب ليش؟ لأن addition of competitive
+
+90
+00:05:48,110 --> 00:05:52,870
+inhibitor increased KM for a given substrate في
+
+91
+00:05:52,870 --> 00:05:56,870
+حال وجود competitive inhibitor بتزيد KM عشان أزود
+
+92
+00:05:56,870 --> 00:06:00,610
+تركيز الـsubstrate عشان الـsubstrate يفوز ويكون
+
+93
+00:06:00,610 --> 00:06:04,130
+تركيزه أعلى من الـcompetitive inhibitor وهو اللي
+
+94
+00:06:04,130 --> 00:06:09,200
+يفوز في موقع الـactive site في الإرتباطفي حالة الـ
+
+95
+00:06:09,200 --> 00:06:11,280
+Competitive Inhibitor يحتاج المزيد من الـ
+
+96
+00:06:11,280 --> 00:06:15,520
+Substrate لإيقافها لوجود Competitive Inhibitor أنا
+
+97
+00:06:15,520 --> 00:06:18,500
+بحتاج إلى مزيد من الـ Substrate عشان يرتبط مع
+
+98
+00:06:18,500 --> 00:06:22,820
+النظيم و هو اللي يفوز و يمشي التفاعل و يوصل فيما
+
+99
+00:06:24,530 --> 00:06:28,810
+أما Vmax ما تتغير سرعة التفاعل أقصى سرعة التفاعل
+
+100
+00:06:28,810 --> 00:06:32,670
+مش هتتغير هي هي في حال وجود inhibitor أو في عدم
+
+101
+00:06:32,670 --> 00:06:36,510
+وجود inhibitor don't competitive inhibitor don't
+
+102
+00:06:36,510 --> 00:06:40,610
+alter the Vmax مابيغيرش Vmax effect of competitive
+
+103
+00:06:40,610 --> 00:06:43,970
+inhibitor is reversed by increasing substrate
+
+104
+00:06:43,970 --> 00:06:47,590
+concentration high substrate concentration the
+
+105
+00:06:47,590 --> 00:06:51,870
+reaction velocity reached the same Vmax as that
+
+106
+00:06:51,870 --> 00:06:57,340
+observed in the absence of the inhibitorمحكي تأثير
+
+107
+00:06:57,340 --> 00:07:01,180
+الـ Competitive Inhibitor بقدر إني أتلاشى وإني
+
+108
+00:07:01,180 --> 00:07:05,440
+أشيلهم من خلال زيادة تركيز الـSubstrate إذا زودت
+
+109
+00:07:05,440 --> 00:07:09,540
+تركيز الـSubstrate، التفاعل بيوصل لـVmax نفس اللي
+
+110
+00:07:09,540 --> 00:07:14,190
+كانت في الأول، بيوصل لأقصى سرعة من التفاعلفي اللي
+
+111
+00:07:14,190 --> 00:07:17,550
+هي نفس السرعة في حال غياب الـInhibitor هاصل
+
+112
+00:07:17,550 --> 00:07:21,430
+الـVmax سواء فيه Inhibitor ولا فاشر بس في حال وجود
+
+113
+00:07:21,430 --> 00:07:25,110
+Inhibitor لازم أزود تركيز الـSubstrate عشان أحصل
+
+114
+00:07:25,110 --> 00:07:29,750
+الـVmax this is because at higher concentration في
+
+115
+00:07:29,750 --> 00:07:34,220
+حال تركيز مرتفعالـ Active site هيتشبع بيه الـ
+
+116
+00:07:34,220 --> 00:07:37,600
+Substrate لأن الـ Substrate هي اللي هي التركيزها
+
+117
+00:07:37,600 --> 00:07:42,180
+أعلى أما الـ Inhibitor cannot bind مش هيرتبط
+
+118
+00:07:42,180 --> 00:07:45,320
+الانتباه التاني من الـ Inhibitor هو الـ
+
+119
+00:07:45,320 --> 00:07:48,380
+irreversible inhibitor أو الـ non competitive
+
+120
+00:07:48,380 --> 00:07:52,070
+inhibitorأحكيلي إن هذا الـ Non-competitive مش
+
+121
+00:07:52,070 --> 00:07:57,210
+تنافسي، كيف مش تنافسي؟ إنه BGN إله موقع خاص موجود
+
+122
+00:07:57,210 --> 00:08:00,890
+على الإنزيم وبيرتبط فيه، غير الـ Active site إيش
+
+123
+00:08:00,890 --> 00:08:04,810
+اسم هذا الموقع؟ اسمه الـ Steric site إن الإنزيم
+
+124
+00:08:04,810 --> 00:08:08,030
+بيكون فيه موقع، موقع الـ Active site اللي بيرتبط
+
+125
+00:08:08,030 --> 00:08:11,630
+فيه الـ Substrate فيه موقع اسمه الـ Steric site
+
+126
+00:08:11,630 --> 00:08:15,470
+بترتبط فيه الـ Non-competitive inhibitorيعني كل
+
+127
+00:08:15,470 --> 00:08:19,510
+واحد له مكانه بيجي بيرطبط في هذا المكان اللي نبته
+
+128
+00:08:19,510 --> 00:08:22,810
+في مكانه و ال substrate في مكانه فبيحكيلي في هذه
+
+129
+00:08:22,810 --> 00:08:26,770
+الحالة أنت لو مهما زوّتي تركيز ال substrate مش
+
+130
+00:08:26,770 --> 00:08:31,230
+هتحل المشكلة لأن هو مش مابيتنافسوا على نفس الموقع
+
+131
+00:08:31,230 --> 00:08:34,550
+هم كل واحد له موقع أنا مهما أزيد تركيز ال
+
+132
+00:08:34,550 --> 00:08:38,930
+substrate مش هحل هذه المشكلة these are not treated
+
+133
+00:08:38,930 --> 00:08:42,030
+by the concentration of substrate ما بتحل المشكلة
+
+134
+00:08:42,030 --> 00:08:42,330
+بزيادة
+
diff --git a/PL9fwy3NUQKwZ1Q7zOg1ObgufPMjRI3WY1/QSsLWVqy8CU.srt b/PL9fwy3NUQKwZ1Q7zOg1ObgufPMjRI3WY1/QSsLWVqy8CU.srt
new file mode 100644
index 0000000000000000000000000000000000000000..36b5760817a020d2e1fec5b18b7c128ec8a44065
--- /dev/null
+++ b/PL9fwy3NUQKwZ1Q7zOg1ObgufPMjRI3WY1/QSsLWVqy8CU.srt
@@ -0,0 +1,830 @@
+1
+00:00:00,000 --> 00:00:04,390
+يكون positive، إذا كان يحتوي، وإلا هيكون negative فكيف
+
+2
+00:00:04,390 --> 00:00:08,530
+الـ principle مبدأ عمل الفحص أنه بيجي عند حمض أميني 
+
+3
+00:00:08,530 --> 00:00:13,290
+amino acid with aromatic ring بيحتوي على حلقة 
+
+4
+00:00:13,290 --> 00:00:17,070
+بنزين بحط عليه concentrated nitric acid
+
+5
+00:00:17,070 --> 00:00:23,130
+concentrated nitric acid HNO3 في وجود قاعدة قوية
+
+6
+00:00:23,130 --> 00:00:27,870
+زي الـ NaO2 في وجود NaO2 ليش هيجي يعمل الـ nitric
+
+7
+00:00:27,870 --> 00:00:32,290
+acid لحمض الأميني اللي بيحتوي على حلقة البنزين؟ بيعمل
+
+8
+00:00:32,290 --> 00:00:37,190
+Nitration of Benzene Ring بيضيف NO2 على حلقة البنزين Nitration of 
+
+9
+00:00:37,190 --> 00:00:42,370
+Benzene Ring بيضيف NO2 على حلقة البنزين Nitric
+
+10
+00:00:42,370 --> 00:00:46,110
+Acid بيضيف NO2 على حلقة البنزين بيسميها Nitration
+
+11
+00:00:46,110 --> 00:00:49,870
+of Benzene Ring إذن هو Amino Acid with Benzene
+
+12
+00:00:49,870 --> 00:00:54,510
+Ring زائد Concentrated Nitric Acid مع Concentrated
+
+13
+00:00:54,510 --> 00:00:59,530
+HNO3 في وجود NaOH بيصير Nitration of Benzene Ring
+
+14
+00:01:02,100 --> 00:01:05,200
+حلقة البنزين بتحتوي على لون أصفر، إذا صار لون أصفر
+
+15
+00:01:05,200 --> 00:01:11,200
+إذا positive، وإذا ماصار لون أصفر إذا negative، إذا لا يحتوي على حلقة البنزين
+
+16
+00:01:11,200 --> 00:01:15,720
+في الملاحظة اللي تحت بيحكيلي عن الأحماض 
+
+17
+00:01:15,720 --> 00:01:18,920
+الأمينية اللي بتحتوي على حلقة البنزين، مين هم الأحماض
+
+18
+00:01:18,920 --> 00:01:22,340
+الأمينية اللي بتحتوي على حلقة البنزين؟ هم ثلاثة، في
+
+19
+00:01:22,340 --> 00:01:27,500
+الـTyrosine وTryptophan وPhenylalanine، ثلاثة أحماض
+
+20
+00:01:27,500 --> 00:01:30,980
+أمنية فقط بتحتوي على حلقة البنزين، هم الـTyrosine
+
+21
+00:01:30,980 --> 00:01:35,030
+وTryptophan وPhenylalanine في حالة التيروزين
+
+22
+00:01:35,030 --> 00:01:38,670
+والتريبتوفان بتكون activated، contain activated
+
+23
+00:01:38,670 --> 00:01:43,330
+حلقة البنزين بتكون نشطة، فبتقدر تتفاعل
+
+24
+00:01:43,330 --> 00:01:47,690
+بسهولة مع الـnitric acid مع الـconcentrated nitric
+
+25
+00:01:47,690 --> 00:01:52,460
+acid بيصير لها نيتراشن، بيعطينا لون أصفر، لكن في حالة
+
+26
+00:01:52,460 --> 00:01:56,680
+الفلن الألانين لا تتفاعل مع نيترك أسد لأنها بتكون
+
+27
+00:01:56,680 --> 00:02:03,800
+حلقة البنزين غير نشطة، فمش هيتفاعل ولا هيعطينا لون أصفر
+
+28
+00:02:03,800 --> 00:02:07,640
+إذن الأحماض الأمينية الثلاثة اللي عندها حلقة بنزين هم
+
+29
+00:02:07,640 --> 00:02:12,260
+التيروزين، Tryptophan، فين الألانين؟ ولكن مين النشطة
+
+30
+00:02:12,260 --> 00:02:15,600
+وهيعطينا positive مع فحص ال Xanthopretic test بس
+
+31
+00:02:15,600 --> 00:02:20,240
+التيروزين والـ Tryptophan، أما الفين الألانين هيعطينا 
+
+32
+00:02:20,240 --> 00:02:25,100
+negative، مش هيتغير اللون لأن حلقة البنزين غير نشطة
+
+33
+00:02:25,680 --> 00:02:28,660
+في فحص الـ xantoproteic test من هيعطينا positive
+
+34
+00:02:28,660 --> 00:02:35,280
+فقط التيروزين والتريبتوفان، وكمان أي بروتين بيحتوي 
+
+35
+00:02:35,280 --> 00:02:39,780
+على التيروزين أو التريبتوفان في تركيبه، فالبروتين 
+
+36
+00:02:39,780 --> 00:02:42,980
+هو عبارة عن مجموعة من الأحماض الأمنية مرتبطة مع
+
+37
+00:02:42,980 --> 00:02:47,080
+بعضها البعض بروابط ببتيدية، إذا كان أحد الأحماض
+
+38
+00:02:47,080 --> 00:02:50,880
+الأمينية هذه تيروزين أو تريبتوفان أو الاثنين مع بعض
+
+39
+00:02:50,880 --> 00:02:57,410
+هيعطينا positive، أوضح بمعادلة إن الـ Thyrosine مع
+
+40
+00:02:57,410 --> 00:03:00,270
+Concentrated Nitric Acid، مش مهم إن الـ Structure
+
+41
+00:03:00,270 --> 00:03:04,810
+إنه بيعطينا Nitration of حلقة البنزين، Yellow Color
+
+42
+00:03:04,810 --> 00:03:08,130
+وإذا تفاعل برضه الـ Tryptophan مع Concentrated
+
+43
+00:03:08,130 --> 00:03:11,770
+Nitric Acid، هيصير برضه عملية Nitration of حلقة البنزين
+
+44
+00:03:11,770 --> 00:03:14,750
+وهيعطينا Yellow Color، لأن الـ Thyrosine والـ 
+
+45
+00:03:14,750 --> 00:03:17,930
+Tryptophan بيحتوي على Activated Benzene Ring، لكن لو
+
+46
+00:03:17,930 --> 00:03:21,190
+تفاعلنا الفل الأليان مع الـ Concentrated Nitric
+
+47
+00:03:21,190 --> 00:03:25,530
+Acid ما بيتفعلش، مش هيعطينا Yellow Color، ليش؟ لأن
+
+48
+00:03:25,530 --> 00:03:28,990
+حلقة البنزين اللي موجودة في الفل الألانين غير
+
+49
+00:03:28,990 --> 00:03:30,950
+نشطة، من أكثر
+
+50
+00:03:33,620 --> 00:03:38,140
+طب كيف خطوات العمل؟ خطوات العمل بجيب أنبوبة، glass
+
+51
+00:03:38,140 --> 00:03:41,680
+tube، بحط عليها 1 مل من الحمض الأميني أو من
+
+52
+00:03:41,680 --> 00:03:44,140
+المحلول اللي أنا أعطيتكياه، أنا مش عارف حمض أميني 
+
+53
+00:03:44,140 --> 00:03:47,400
+أو بروتين، المهم محلول اللي أنا أعطيتكياه، بتحط منه
+
+54
+00:03:47,400 --> 00:03:51,000
+1 مل، بتحط عليه 1 مل من ال nitric acid من ال
+
+55
+00:03:51,000 --> 00:03:55,970
+HNO3، بعدين بتسخينه على اللهب، لما نسخن على اللهب
+
+56
+00:03:55,970 --> 00:04:00,390
+بنحطه في الـ rack يبرد، بعد ما تبرد شوية الأنبوبة بنضيف
+
+57
+00:04:00,390 --> 00:04:04,130
+عليها 2 مل من ال NaOH من القاعدة، ليش هذه
+
+58
+00:04:04,130 --> 00:04:08,330
+ال NaOH؟ عشان تزود شدة اللون، بنضيف NaOH
+
+59
+00:04:08,330 --> 00:04:13,590
+لزيادة شدة اللون، والله إذا تغير اللون إلى أصفر
+
+60
+00:04:13,590 --> 00:04:18,310
+إذن هذا حمض أميني أو بروتين بيحتوي على حلقة بنزين
+
+61
+00:04:18,310 --> 00:04:23,090
+إذا ما تغيرش اللون، إذن لا يحتوي على حلقة بنزين
+
+62
+00:04:25,930 --> 00:04:29,010
+الفحص اللي بعد هيك هو الـ Hopkin Cole أو الـ
+
+63
+00:04:29,010 --> 00:04:32,730
+Glyoxylic Acid Reaction، اللي هو اسمها؟ Hopkin
+
+64
+00:04:32,730 --> 00:04:36,250
+Cole أو Glyoxylic Acid Reaction، إيش الهدف من
+
+65
+00:04:36,250 --> 00:04:41,130
+الفحص؟ لكشف عن وجود التريبتوفان، بيكشف عن
+
+66
+00:04:41,130 --> 00:04:43,950
+التريبتوفان، مش معناه التريبتوفان، لأنه هذا بيدور على
+
+67
+00:04:43,950 --> 00:04:49,730
+حاجة بنسميها Indole Group، هي مجموعة موجودة بس في الـ 
+
+68
+00:04:49,730 --> 00:04:52,870
+tryptophan، إذا موجود ال tryptophan هيعطينا
+
+69
+00:04:52,870 --> 00:04:55,970
+positive، إذا مش موجود هيعطينا negative، فهو
+
+70
+00:04:55,970 --> 00:04:59,790
+positive مع ال tryptophan أو أي بروتين بيحتوي في
+
+71
+00:04:59,790 --> 00:05:03,990
+تركيبه على ال tryptophan، هبقى الـ principle المبدأ
+
+72
+00:05:03,990 --> 00:05:07,410
+إنه بجيب الحمض الأميني اللي عندي ال tryptophan أو
+
+73
+00:05:07,410 --> 00:05:13,330
+البروتين وبضيف عليه Glyoxylic Acid Reagent مع H2SO4
+
+74
+00:05:13,330 --> 00:05:17,400
+concentrated sulfuric acid، بتكون لي هذا الـ
+
+75
+00:05:17,400 --> 00:05:20,360
+Sulfuric Acid هي مسؤولة عن تكوين، to formation of 
+
+76
+00:05:20,360 --> 00:05:24,420
+violet ring، بتكون لي حلقة في الوسط، بتفصل solution
+
+77
+00:05:24,420 --> 00:05:28,960
+من الأعلى لأسفل، بتكون حلقة لونها بنفسجي، violet ring، 
+
+78
+00:05:28,960 --> 00:05:32,600
+الـ Sulfuric Acid بتكون لي violet ring، بتكون لي
+
+79
+00:05:32,600 --> 00:05:36,900
+حلقة لونها بنفسجي، إذا تكونت هذه الحلقة معناها إنه
+
+80
+00:05:36,900 --> 00:05:40,100
+الفحص positive، معناه إنه هذا المركب هو tryptophan
+
+81
+00:05:40,100 --> 00:05:45,440
+أو بروتين بيحتوي على tryptophan، إذا لم تتكون، إذا أنه
+
+82
+00:05:45,440 --> 00:05:52,040
+negative، لا يحتوي على الـ tryptophan، طب 
+
+83
+00:05:52,040 --> 00:05:55,520
+كيف ال procedure في وقت العمل؟ بجيب الأنبوبة glass tube
+
+84
+00:05:55,520 --> 00:05:58,900
+بحط فيها 1 مل من ال solution من المحلول اللي
+
+85
+00:05:58,900 --> 00:06:02,560
+أنا أعطيتكياه سواء tryptophan أو غيرها وبحط عليها 
+
+86
+00:06:02,560 --> 00:06:06,460
+1 مل من ال Glyoxylic Acid Reagent وبعمل mix
+
+87
+00:06:06,460 --> 00:06:09,800
+بجيب الأنبوبة وبحط عليها 1 مل من ال solution 
+
+88
+00:06:09,800 --> 00:06:13,630
+مع 1 مل من ال Glyoxylic Acid Reagent وبعمل mix، بعدين
+
+89
+00:06:13,630 --> 00:06:18,630
+بمسك الأنبوبة بميلها بشكل مائل وبحط فيها من 3 إلى 4 
+
+90
+00:06:18,630 --> 00:06:23,510
+مل من ال H2SO4 Concentrated Sulphuric Acid على 
+
+91
+00:06:23,510 --> 00:06:26,930
+الجدار، بنضيف ال Sulphuric Acid وبشكل تدريجي مش 
+
+92
+00:06:26,930 --> 00:06:31,750
+مرة واحدة وبحرص على إني ما أرجش الأنبوبة، بمسك الأنبوبة
+
+93
+00:06:31,750 --> 00:06:35,890
+بشكل مايل وبضيف على الجدار من 3 إلى 4 مل من ال
+
+94
+00:06:35,890 --> 00:06:39,770
+concentrated sulphuric acid بشكل تدريجي بدون رج
+
+95
+00:06:39,770 --> 00:06:44,510
+للأنبوبة، بعد ما أخلص بآعدل الأنبوبة شوية شوية بدون ما أرجها 
+
+96
+00:06:44,510 --> 00:06:49,630
+بآعدلها، إذا تكونت عندي حلقة لونها بنفسجي، إذن هذا 
+
+97
+00:06:49,630 --> 00:06:52,670
+positive، بيحتوي على الـ tryptophan، هو tryptophan،
+
+98
+00:06:52,670 --> 00:06:55,550
+يمكن أن يكون الـ tryptophan أو protein بيحتوي على
+
+99
+00:06:55,550 --> 00:06:58,410
+tryptophan، إذا ما تكونتش الحلقة، إذا الـ
+
+100
+00:06:58,410 --> 00:07:02,430
+negative، لا يوجد tryptophan، لا يحتوي على الـ
+
+101
+00:07:02,430 --> 00:07:03,230
+tryptophan
+
+102
+00:07:06,060 --> 00:07:09,800
+بس اللي بعد هيك هو ال Millon's test، ال Millon's test
+
+103
+00:07:09,800 --> 00:07:14,120
+هو للكشف عن وجود التيروزين، objective to detect the
+
+104
+00:07:14,120 --> 00:07:17,200
+presence of tyrosine، ليش؟ لأن التيروزين بيحتوي على
+
+105
+00:07:17,200 --> 00:07:19,600
+Phenol Group، هو بالذات ال Phenol Group اللي في
+
+106
+00:07:19,600 --> 00:07:23,160
+التيروزين، إذا موجود أنا تيروزين عنده هيعطينا
+
+107
+00:07:23,160 --> 00:07:26,240
+positive، إذا مش موجود هيعطينا negative، فهو 
+
+108
+00:07:26,240 --> 00:07:30,260
+positive مع التيروزين أو أي بروتين بيحتوي على
+
+109
+00:07:30,260 --> 00:07:34,820
+التيروزين، غير هيك بيعطينا negative، إيش هي الـ
+
+110
+00:07:34,820 --> 00:07:39,900
+Phenol Group؟ هي حلقة بنزين مرتبطة بـ OH، Hydroxyl
+
+111
+00:07:39,900 --> 00:07:43,300
+Group Attached to Benzene Ring، إذا عندي حلقة بنزين
+
+112
+00:07:43,300 --> 00:07:46,640
+مرتبطة بـ Hydroxyl Group، فهذه بنسميها Phenol
+
+113
+00:07:46,640 --> 00:07:50,840
+Group، وهي موجودة في حالة التيروزين، بيحكي للـ Millon's
+
+114
+00:07:50,840 --> 00:07:55,210
+Reagent عبارة عن مكونات بتحتوي على mercury وNitric
+
+115
+00:07:55,210 --> 00:07:59,110
+acid، HNO3 و Mercury، وهذول المكونين هم 
+
+116
+00:07:59,110 --> 00:08:03,710
+toxic و corrosive و بسبب حروق ومُهيج irritant
+
+117
+00:08:03,710 --> 00:08:10,330
+فبدنا نتعامل مع ال Mercury reaction بحذر، طب إيش
+
+118
+00:08:10,330 --> 00:08:11,270
+الـ principle اللي 
+
+119
+00:08:19,710 --> 00:08:22,810
+التيروسين مع الـMillon's reagent، حكينا الـMillon's
+
+120
+00:08:22,810 --> 00:08:26,670
+reagent هو عبارة عن مكونات Nitric Acid، HNO3،  وMercury
+
+121
+00:08:26,670 --> 00:08:30,410
+مجرد ما أضيف الـMillon's reagent على التيروسين
+
+122
+00:08:30,410 --> 00:08:35,090
+بتيجي أول مكون اللي هو الـnitric acid بيعمل Nitration
+
+123
+00:08:35,090 --> 00:08:38,930
+لحلقة البنزين، بيعمل Nitration لحلقة البنزين الموجودة في
+
+124
+00:08:38,930 --> 00:08:42,620
+التيروسين، بعد ما يصير لها Nitration بتيجي الـ mercury
+
+125
+00:08:42,620 --> 00:08:47,220
+بيرتبط معها وبيتكون عندي لون أحمر طوبي، إذا إن هو 
+
+126
+00:08:47,220 --> 00:08:51,640
+تيروسين مع Millon's Reagent وبيتكون عندي Nitrated 
+
+127
+00:08:51,640 --> 00:08:55,800
+Tyrosine Mercury Complex، اللي هو التيروسين بيصير له
+
+128
+00:08:55,800 --> 00:08:59,560
+Nitration ومرتبط معه mercury، بنسميها Nitrated Tyrosine
+
+129
+00:08:59,560 --> 00:09:05,160
+Mercury Complex، شو لونه بيكون؟ بيكون لونه أحمر طوبي
+
+130
+00:09:05,160 --> 00:09:08,180
+إذا إن هو positive، إذا إن هو التيروزين أو أي 
+
+131
+00:09:08,180 --> 00:09:12,530
+بروتين بيحتوي على التيروزين، نحكي للملاحظة أن كل
+
+132
+00:09:12,530 --> 00:09:16,690
+الـPhenol Group اللي بتحتوي، كل الـPhenol Group كل المركبات اللي
+
+133
+00:09:16,690 --> 00:09:20,530
+عندها Phenol Group اللي هي حلقة بنزين مرتبطة ب OH
+
+134
+00:09:20,530 --> 00:09:26,190
+هتعطينا positive مع Millon's Reagent، إن إحنا حكينا بس 
+
+135
+00:09:26,190 --> 00:09:28,570
+طب إيش الـ principle
+
+136
+00:09:34,850 --> 00:09:38,270
+التيروسين، التيروسين وبضيف عليه Millon's reagent، التيروسين 
+
+137
+00:09:38,270 --> 00:09:40,950
+مع الـ Millon's reagent، حكينا الـ Millon's reagent هو 
+
+138
+00:09:40,950 --> 00:09:45,790
+عبارة عن مكونات Nitric Acid، HNO3،  وMercury مركز، مجرد
+
+139
+00:09:45,790 --> 00:09:49,130
+ما أضيف الـ Millon's reagent على التيروسين بتيجي أول
+
+140
+00:09:49,130 --> 00:09:52,710
+إيه؟ أول مكون اللي هو الـ nitric acid بيعمل Nitration 
+
+141
+00:09:52,710 --> 00:09:56,560
+لحلقة البنزين، بيعمل Nitration لحلقة البنزين الموجودة في
+
+142
+00:09:56,560 --> 00:09:59,980
+الـ Tyrosine، بعد ما يصير لها Nitration، بتيجي الـ
+
+143
+00:09:59,980 --> 00:10:04,440
+Mercury بيرتبط معها وبيتكون عندي لون أحمر طوبي، 
+
+144
+00:10:04,440 --> 00:10:08,280
+إذا إن هو Tyrosine مع Millon's Reagent، بيتكون عندي
+
+145
+00:10:08,280 --> 00:10:12,860
+Nitrated Tyrosine Mercury Complex، اللي هو التيروسين
+
+146
+00:10:12,860 --> 00:10:15,760
+بيصير له Nitration ومرتبط معاه Mercury، بنسميها
+
+147
+00:10:15,760 --> 00:10:19,960
+Nitrated Tyrosine Mercury Complex، شو لونه؟ بيكون
+
+148
+00:10:19,960 --> 00:10:24,560
+لونه أحمر طوبي، إذا إن هو positive، إذا إن
+
+149
+00:10:24,560 --> 00:10:28,860
+هو التيروسين أو أي بروتين بيحتوي على التيروزين، بنحكي
+
+150
+00:10:28,860 --> 00:10:33,420
+للملاحظة أن كل الـPhenol Group اللي بتحتوي، كل الـPhenol Group، كل
+
+151
+00:10:33,420 --> 00:10:37,080
+المركبات اللي عندها Phenol Group، هي حلقة بنزين
+
+152
+00:10:37,080 --> 00:10:41,800
+مرتبطة ب OH هتعطينا positive مع Millon's Reagent، إن إحنا حكينا بس 
+
+153
+00:10:41,800 --> 00:10:45,460
+من الأحماض الأمنية التيروسين هو
+
+154
+00:10:45,460 --> 00:10:48,400
+الوحيد اللي عنده Phenol Group، عنده حلقة بنزين
+
+155
+00:10:48,400 --> 00:10:52,400
+مرتبطة ب OH، لكن هل الطبيعة كلها في المركبات
+
+156
+00:10:52,400 --> 00:10:56,000
+الكيميائية كلها، فيش غير التيروسين صح؟ وفي الأحماض 
+
+157
+00:10:56,000 --> 00:11:01,350
+الأمينية بس، ثاني فيه مركبات تانية عندها حلقة بنزين
+
+158
+00:11:01,350 --> 00:11:04,970
+مرتبطة بـ OH، لو جيت اشتغلت عليها الـ Millon's test
+
+159
+00:11:04,970 --> 00:11:09,010
+هتعطينا positive، طب على فرض أنا أعطيتك محلول 
+
+160
+00:11:09,010 --> 00:11:12,810
+وحكيت لك شوفي لي إيش هذا المحلول، شغلي لي عليه الـ 
+
+161
+00:11:12,810 --> 00:11:16,630
+Millon's، شوفي تايروسين positive ولا negative، فجيت إن أنتِ 
+
+162
+00:11:16,630 --> 00:11:20,330
+اشتغلتيه وأعطاكي positive، إذن هي هتتوقع إنه مركب
+
+163
+00:11:20,330 --> 00:11:24,960
+بيحتوي على Phenol Group تمام؟ طب هل هو التيروسين ولا
+
+164
+00:11:24,960 --> 00:11:29,400
+مركب تاني؟ كيف بتتأكد هل هو التيروسين أو لا؟ لأن 
+
+165
+00:11:29,400 --> 00:11:32,920
+في مركبات تانية بتحتوي على Phenol Group؟ بشتغل
+
+166
+00:11:32,920 --> 00:11:36,720
+فحص الـNinhydrin، مش الـNinhydrin أول فحص أخذناه هو
+
+167
+00:11:36,720 --> 00:11:40,980
+مخصص لـ Free Alpha Amino Group اللي موجودة في كل
+
+168
+00:11:40,980 --> 00:11:45,310
+الأحماض الأمنية، الـAmino Acid، أنا هشتغل فحص الـ
+
+169
+00:11:45,310 --> 00:11:49,610
+Ninhydrin، لما تشتغلي فحص الـ Ninhydrin إذا أعطاكي
+
+170
+00:11:49,610 --> 00:11:52,810
+positive إذن هو التيروسين، لأن حمض أميني بيحتوي على
+
+171
+00:11:52,810 --> 00:11:55,830
+Free Alpha Amino Group، إذا أعطاني negative إذن هو
+
+172
+00:11:55,830 -->
+
+201
+00:13:51,280 --> 00:13:55,280
+فغير قادر على كسر الرابط وأنه يتفاعل فهو negative
+
+202
+00:13:55,280 --> 00:13:58,540
+في حالة الميثيونين و positive في حالة السيستين
+
+203
+00:13:58,540 --> 00:14:04,180
+والسيستين وأي بروتين يحتوي عليه مهم إذا للـ
+
+204
+00:14:04,180 --> 00:14:06,700
+lead sulphide test أو الـ sulphur test اللي كاشف
+
+205
+00:14:06,700 --> 00:14:10,040
+عن ال sulphohydryl group الموجودة في الميثيونين أو ال
+
+206
+00:14:10,040 --> 00:14:14,340
+ميثيونين نيجاتيف لإنها not freeماشي ال principle
+
+207
+00:14:14,340 --> 00:14:18,980
+أني بجيب الحمض الأميني مثلًا سيستين أو سستيان بضيف
+
+208
+00:14:18,980 --> 00:14:24,480
+عليها 10% من ال NaOH في وجود حرارة heat بيعطيني
+
+209
+00:14:24,480 --> 00:14:30,440
+Sodium Sulphate بجيب سيستين أو سيستان مع 10% NaOH 
+
+210
+00:14:30,440 --> 00:14:34,880
+في وجود حرارة بتكون عندي Sodium Sulphate بضيف
+
+211
+00:14:34,880 --> 00:14:40,060
+عليها 10% Lead Acetate بيعطيني حاجة بتسميها Lead
+
+212
+00:14:40,060 --> 00:14:44,260
+Sulphate الليد سالفايت هذا عبارة عن ال precipitates
+
+213
+00:14:44,260 --> 00:14:50,260
+لونه أسود، سيستين أو سيستان، مع 10% النيوهاتش في وجود
+
+214
+00:14:50,260 --> 00:14:55,180
+حرارة، بيعطيني Sodium Sulfate، بضيف عليه 10% ليد
+
+215
+00:14:55,180 --> 00:14:59,360
+أسيتات، بيعطيني Lead Sulfide ب precipitates لونه
+
+216
+00:14:59,360 --> 00:15:03,720
+أسود، يعني positive، يعني بتحتوي على سيستين أو سيستان 
+
+217
+00:15:07,940 --> 00:15:11,080
+هنا بيوضح لي أن الميثيونين والسيستين الاثنين
+
+218
+00:15:11,080 --> 00:15:14,240
+بيحتوي على sulfur group بس ليش الميثيونين بس هي اللي
+
+219
+00:15:14,240 --> 00:15:17,300
+بتعطيني positive لو جينا لل structure الميثيونين 
+
+220
+00:15:17,300 --> 00:15:22,380
+تشوفوا ال S و N مرتبطة في CH2 من جهة و CH3 من جهة
+
+221
+00:15:22,380 --> 00:15:25,880
+فهي مرتبطة من الجهتين وصعب تكسير هذه الرابطة
+
+222
+00:15:26,100 --> 00:15:29,880
+فبنقدرش نكسرها عشان تتفاعل ما يتفاعل فهي not free
+
+223
+00:15:29,880 --> 00:15:33,980
+أما في حالة السيستين فهي free هي  ال S فوق 
+
+224
+00:15:33,980 --> 00:15:37,860
+مرتبطة في H من جهة و free من جهة أخرى تقدر تتفاعل
+
+225
+00:15:37,860 --> 00:15:42,780
+وتحت معادلات لبرنسبل الفحص السيستين مضيف عليه
+
+226
+00:15:42,780 --> 00:15:47,180
+النيوهاتش في وجود حرارة بيعطيني صوديوم سالفايت
+
+227
+00:15:47,180 --> 00:15:52,240
+مضيف عليها 10% من lead أسيتات بيعطيني lead
+
+228
+00:15:52,240 --> 00:15:56,600
+سالفايت ال precipitate لونه أسوديكون ال recipe
+
+229
+00:15:56,600 --> 00:16:02,600
+اللي هو هنا اسمه كيف خطوات العمل؟ بحط 1 مل من ال
+
+230
+00:16:02,600 --> 00:16:06,300
+solution أنا أعطيتك إياه في تيووب بحط 1 مل منه على
+
+231
+00:16:06,300 --> 00:16:10,480
+1 مل من ال sodium hydroxide ده نيو هاتش مع خمسة
+
+232
+00:16:10,480 --> 00:16:13,580
+دروب من عشرة فالمية lead acetate بحطهم كلهم مع بعض
+
+233
+00:16:13,580 --> 00:16:17,160
+وبعمل math بعدين بحطه في ال water bath لمدة خمسة
+
+234
+00:16:17,160 --> 00:16:21,020
+دقائق بطلعه من ال water bath إذا صار لونه أسود فيه
+
+235
+00:16:21,020 --> 00:16:24,400
+راسب أسود is a positive يحتوي على ال system إذا
+
+236
+00:16:24,400 --> 00:16:28,940
+ما اتغيرش اللون is a negative ليحتوي على ال system 
diff --git a/PL9fwy3NUQKwZ1Q7zOg1ObgufPMjRI3WY1/QSsLWVqy8CU_raw.srt b/PL9fwy3NUQKwZ1Q7zOg1ObgufPMjRI3WY1/QSsLWVqy8CU_raw.srt
new file mode 100644
index 0000000000000000000000000000000000000000..31a3b1182e3e6f9801100642d427a8e73f6b6226
--- /dev/null
+++ b/PL9fwy3NUQKwZ1Q7zOg1ObgufPMjRI3WY1/QSsLWVqy8CU_raw.srt
@@ -0,0 +1,944 @@
+1
+00:00:00,000 --> 00:00:04,390
+يكون positive، إذا لا يحتوي، هيكون negativeفكيف
+
+2
+00:00:04,390 --> 00:00:08,530
+الـ principle مبدأ عمل الفحص أنه بيجي عنده amino
+
+3
+00:00:08,530 --> 00:00:13,290
+acid with aromatic ring حمض أميني بيحتوي على حلقة
+
+4
+00:00:13,290 --> 00:00:17,070
+بنزين بحط عليه concentrated nitric acid
+
+5
+00:00:17,070 --> 00:00:23,130
+concentrated nitric acid HNO3 في وجود قاعدة قوية
+
+6
+00:00:23,130 --> 00:00:27,870
+زي الـ NaO2 في وجود NaO2 ليش هييجي يعمل الـ nitric
+
+7
+00:00:27,870 --> 00:00:32,290
+acid لحمض الأميني اللي يحتوي على بنزين ring؟بيعمل
+
+8
+00:00:32,290 --> 00:00:37,190
+Nitration of Benzene Ring بيضيف Nitration of
+
+9
+00:00:37,190 --> 00:00:42,370
+Benzene Ring بيضيف NO3 على حلقة البنزين Nitric
+
+10
+00:00:42,370 --> 00:00:46,110
+Acid بيضيف NO3 على حلقة البنزين بيسميها Nitration
+
+11
+00:00:46,110 --> 00:00:49,870
+of Benzene Ring إذن هو Amino Acid with Benzene
+
+12
+00:00:49,870 --> 00:00:54,510
+Ring زائد Concentrated Nitric Acid مع Concentrated
+
+13
+00:00:54,510 --> 00:00:59,530
+HNO3 في وجود NOH بيصير Nitration of Benzene Ring
+
+14
+00:01:02,100 --> 00:01:05,200
+بنزين ريال يحتوي على يلو color إذا صار يلو color
+
+15
+00:01:05,200 --> 00:01:11,200
+إذا positive إذا negative إذا لا يحتوي على بنزين
+
+16
+00:01:11,200 --> 00:01:15,720
+ريالفي الملاحظة اللي تحت بيحكيلي عن الأحماض
+
+17
+00:01:15,720 --> 00:01:18,920
+الأمنية اللي بتحتوي على بنزين رينج، مين هم الأحماض
+
+18
+00:01:18,920 --> 00:01:22,340
+الأمنية اللي بتحتوي على بنزين رينج؟ هم تلات، في
+
+19
+00:01:22,340 --> 00:01:27,500
+الـTyrosine وTryptophan وPhenylalanine، تلت أحماض
+
+20
+00:01:27,500 --> 00:01:30,980
+أمنية فقط تحتوي على بنزين رينج، هم الـTyrosine
+
+21
+00:01:30,980 --> 00:01:35,030
+وTryptophan وPhenylalanineفي حالة التيروزين
+
+22
+00:01:35,030 --> 00:01:38,670
+والتريبتوفان بتكون activated، contain activated
+
+23
+00:01:38,670 --> 00:01:43,330
+بنزين ريكو حلقة البنزين بتكون نشطة فبتقدر تتفاعل
+
+24
+00:01:43,330 --> 00:01:47,690
+بسهولة مع الـnitric acid مع الـconcentrated nitric
+
+25
+00:01:47,690 --> 00:01:52,460
+acid بيصيريلها نيتراشن بيعطين لون يلولكن في حالة
+
+26
+00:01:52,460 --> 00:01:56,680
+الفلن الألانين لا تتفاعل مع نيترك أسن لأنها بتكون
+
+27
+00:01:56,680 --> 00:02:03,800
+حلقة البنزين غير ناشطة فمش هيتفاعل ولا هيعطيني لهم
+
+28
+00:02:03,800 --> 00:02:07,640
+إذا الأحمض الأمينية تلاتة عند حلقة البنزين هم
+
+29
+00:02:07,640 --> 00:02:12,260
+التيروزينTryptophan فين الألانين؟ ولكن مين النشطة
+
+30
+00:02:12,260 --> 00:02:15,600
+و هيعطيني positive مع فحص ال Xanthopretic test بس
+
+31
+00:02:15,600 --> 00:02:20,240
+تيروزين لـ Tryptophan أما الفين الألانين هيعطيني
+
+32
+00:02:20,240 --> 00:02:25,100
+negative مش هيتغير اللون لأن حلقة البنزين غير نشطة
+
+33
+00:02:25,680 --> 00:02:28,660
+فى فحص الـ xantoproteic test من هيعطينى positive
+
+34
+00:02:28,660 --> 00:02:35,280
+فقط التيروزين والتريبتوفان وكمان أي بروتيل يحتوي
+
+35
+00:02:35,280 --> 00:02:39,780
+على التيروزين أو التريبتوفان فى تركيبه فالبروتين
+
+36
+00:02:39,780 --> 00:02:42,980
+هو عبارة عن مجموعة من الأحماض الأمنية مرتبطة مع
+
+37
+00:02:42,980 --> 00:02:47,080
+بعضها البعض بروابط مبتدية إذا كان أحد الأحماض
+
+38
+00:02:47,080 --> 00:02:50,880
+الأمنية هذه تيروزين أو تريبتوفان أو الأثنين معاها
+
+39
+00:02:50,880 --> 00:02:57,410
+هيعطينى positiveأوضح بمعادلة إن الـ Thyrosine مع
+
+40
+00:02:57,410 --> 00:03:00,270
+Concentrated Nitric Acid مش مهم إن الـ Structure
+
+41
+00:03:00,270 --> 00:03:04,810
+إنه يعطيني Nitration of بنزين رنج Yellow Color
+
+42
+00:03:04,810 --> 00:03:08,130
+وإذا اتفاعل برضه الـ Tryptophan مع Concentrated
+
+43
+00:03:08,130 --> 00:03:11,770
+Nitric Acid هيصير برضه عمليت Nitration of بنزين
+
+44
+00:03:11,770 --> 00:03:14,750
+رنج وهيعطيني Yellow Color لأن الـ Thyrosine و الـ
+
+45
+00:03:14,750 --> 00:03:17,930
+Tryptophan يحتوي على Activated Benzene Ring لكن لو
+
+46
+00:03:17,930 --> 00:03:21,190
+اتفاعلنا الفل الأليان مع الـ Concentrated Nitric
+
+47
+00:03:21,190 --> 00:03:25,530
+Acid مايتفعلش، مش هيعطيني Yellow Colorليش؟ لأن
+
+48
+00:03:25,530 --> 00:03:28,990
+حلقة البنزين اللي موجودة في الفيلم الألانين غير
+
+49
+00:03:28,990 --> 00:03:30,950
+ناشطة، من أكتر
+
+50
+00:03:33,620 --> 00:03:38,140
+طب كيف خطوات العمل؟ خطوات العمل بجيب لتيوب، glass
+
+51
+00:03:38,140 --> 00:03:41,680
+tube، بحط عليها واحد مل من الحمض الأميني أو من
+
+52
+00:03:41,680 --> 00:03:44,140
+المحلول اللي أنا أعطاتكيا، أنا مش عارف حمض أميني
+
+53
+00:03:44,140 --> 00:03:47,400
+أو بروتين، مهم محلول اللي أنا أعطاتكيا، بتحط منه
+
+54
+00:03:47,400 --> 00:03:51,000
+واحد مل، بتحط عليه واحد مل من ال nitric acid من ال
+
+55
+00:03:51,000 --> 00:03:55,970
+HNO3، بعدين بتسخنيه على اللهةلما نيسخن على اللهب
+
+56
+00:03:55,970 --> 00:04:00,390
+بحطه في الراك يبرد بعد ما تبرد شوية لـ tube بضيف
+
+57
+00:04:00,390 --> 00:04:04,130
+عليها اتنين نيل من الانهيواتش من القاعدة ليش هذه
+
+58
+00:04:04,130 --> 00:04:08,330
+الانهيواتش؟ عشان تزود شدة اللون بضيف انهيواتش
+
+59
+00:04:08,330 --> 00:04:13,590
+لزيادة شدة اللون والله إذا تغير اللون إلى yellow
+
+60
+00:04:13,590 --> 00:04:18,310
+إذا هذا حمض أميني أو بروتين يحتوي على بنزين ريل
+
+61
+00:04:18,310 --> 00:04:23,090
+إذا ماتغيرش اللون إذا لا يحتوي على بنزين ريل
+
+62
+00:04:25,930 --> 00:04:29,010
+الفحص اللي بعد هيك هو الـ Hopkin Cool أو الـ
+
+63
+00:04:29,010 --> 00:04:32,730
+Glycosilic Acid Reaction اللي هو اسمها؟ Hopkin
+
+64
+00:04:32,730 --> 00:04:36,250
+Cool أو Glycosilic Acid Reaction إيش الهدف من
+
+65
+00:04:36,250 --> 00:04:41,130
+الفحص؟ لكشف عن وجود لتريبتوفعل بيكشف عن
+
+66
+00:04:41,130 --> 00:04:43,950
+لتريبتوفعل، مش معناه لتريبتوفعل لأنه هذا بيدور على
+
+67
+00:04:43,950 --> 00:04:49,730
+حاجة نسميهاالدول جروب هي مجموع موجودة بس في الـ
+
+68
+00:04:49,730 --> 00:04:52,870
+tryptophan إذا موجود ال tryptophan هيعطيني
+
+69
+00:04:52,870 --> 00:04:55,970
+positive إذا مش موجود هيعطيني negative فهو
+
+70
+00:04:55,970 --> 00:04:59,790
+positive مع ال tryptophan أو أي بروتين يحتوي في
+
+71
+00:04:59,790 --> 00:05:03,990
+تركيبه على ال tryptophan هبقاش ال principle المبدأ
+
+72
+00:05:03,990 --> 00:05:07,410
+إنه بجيب الحمض الأمين اللي عندسي ال tryptophan أو
+
+73
+00:05:07,410 --> 00:05:13,330
+البروتين و أضيف عليه cooking pool reagent مع H2SO4
+
+74
+00:05:13,330 --> 00:05:17,400
+concentrated sulfuric acidبتكوّنلي هذا الـ
+
+75
+00:05:17,400 --> 00:05:20,360
+Sulfuric Acid هي مسؤولة عن تكوين to formation of
+
+76
+00:05:20,360 --> 00:05:24,420
+violet ring بتكوّنلي حلقة في الوسط، تفصل solution
+
+77
+00:05:24,420 --> 00:05:28,960
+من الأيلى جزاين، بتكون حلقة لون هموم، violet ring،
+
+78
+00:05:28,960 --> 00:05:32,600
+الـ Sulfuric Acid بتكوّنلي violet ring، بتكوّنلي
+
+79
+00:05:32,600 --> 00:05:36,900
+حلقة لون هموم، إذا اتكوّنت هذه الحلقة، معناها إنه
+
+80
+00:05:36,900 --> 00:05:40,100
+فحص positive، معناه إنه هذا المركب هو tryptophan
+
+81
+00:05:40,100 --> 00:05:45,440
+أو بروتين يحتوي على tryptophanإذا لم تتكون إذا أنه
+
+82
+00:05:45,440 --> 00:05:52,040
+نيجاتيف لا يحتوي على الـ tryptophan طب
+
+83
+00:05:52,040 --> 00:05:55,520
+كيف ال procedure في وقت العمل؟ بجيب ال class tube
+
+84
+00:05:55,520 --> 00:05:58,900
+بحط فيها واحد mil من ال solution من المحلول اللي
+
+85
+00:05:58,900 --> 00:06:02,560
+انا اعطيتكيها سواء tryptophan او غيرها و بحط عليها
+
+86
+00:06:02,560 --> 00:06:06,460
+واحد mil من ال hook and pull reaction و بعمل mix
+
+87
+00:06:06,460 --> 00:06:09,800
+أجيب ال tube و بحط عليها واحد mil من ال solution
+
+88
+00:06:09,800 --> 00:06:13,630
+مع واحد mil من ال hook and pull و بعمل mixبعدين
+
+89
+00:06:13,630 --> 00:06:18,630
+بمسك التيوب بميلها بشكل مائل و بحط فيها من 3 إلى 4
+
+90
+00:06:18,630 --> 00:06:23,510
+مل من ال H2SO4 Concentrated Sulphuric Acid على
+
+91
+00:06:23,510 --> 00:06:26,930
+الجدار بضيفه ال Sulphuric Acid و بشكل تدريجي مش
+
+92
+00:06:26,930 --> 00:06:31,750
+مرة واحدة و بحرص على أني ما أرجش التيوببمسك لتيوب
+
+93
+00:06:31,750 --> 00:06:35,890
+بشكل ما واضيف على الجدار من 3 إلى 4 مل من ال
+
+94
+00:06:35,890 --> 00:06:39,770
+concentrated sulphuric acid بشكل تدريجي بدون رج
+
+95
+00:06:39,770 --> 00:06:44,510
+لتيوب بعد ما اخلص باعدل لتيوب شواش بدون معرجها
+
+96
+00:06:44,510 --> 00:06:49,630
+باعدلها إذا دونت عندي حلقة لونها violetإذا هذا
+
+97
+00:06:49,630 --> 00:06:52,670
+positive، يحتوي على الـ tryptophan، هو tryptophan،
+
+98
+00:06:52,670 --> 00:06:55,550
+يمكن أن يناسب الـ tryptophan أو protein يحتوي على
+
+99
+00:06:55,550 --> 00:06:58,410
+tryptophan، إذا ما اتكونتش الحلقة، إذا الـ
+
+100
+00:06:58,410 --> 00:07:02,430
+negative، لا يوجد tryptophan، لا يحتوي على الـ
+
+101
+00:07:02,430 --> 00:07:03,230
+tryptophan
+
+102
+00:07:06,060 --> 00:07:09,800
+بس اللي بعد هيك هو ال melons test ال melons test
+
+103
+00:07:09,800 --> 00:07:14,120
+هو للكشف عن وجود التيروزين objective to detect the
+
+104
+00:07:14,120 --> 00:07:17,200
+presence of tyrosine ليش؟ لأن التيروزين يحتوي على
+
+105
+00:07:17,200 --> 00:07:19,600
+phenol group هو بالت ال phenol group اللي في
+
+106
+00:07:19,600 --> 00:07:23,160
+التيروزين إذا موجود أنا تيروزين عنده هيعطيني
+
+107
+00:07:23,160 --> 00:07:26,240
+positive إذا مش موجود هيعطيني negative فهو
+
+108
+00:07:26,240 --> 00:07:30,260
+positive مع التيروزين أو أي بروتين يحتوي على
+
+109
+00:07:30,260 --> 00:07:34,820
+التيروزين غير هيك بيعطيني negativeايش هي الـ
+
+110
+00:07:34,820 --> 00:07:39,900
+Phenol Group؟ هي حلقة بنزين مرتبطة بـ OH، Hydroxyl
+
+111
+00:07:39,900 --> 00:07:43,300
+Group Attached to Benzene Ring إذا عندى حلقة بنزين
+
+112
+00:07:43,300 --> 00:07:46,640
+مرتبطة بـ Hydroxyl Group، فهذه بيسميها Phenol
+
+113
+00:07:46,640 --> 00:07:50,840
+Group، وهي موجودة في حالة تيروزين، بيحكي للـ Melon
+
+114
+00:07:50,840 --> 00:07:55,210
+Reagent عبارة عن مكونات contain mercury andNitric
+
+115
+00:07:55,210 --> 00:07:59,110
+acid هتش أنوادري و Mercury و هدول المكونين هم
+
+116
+00:07:59,110 --> 00:08:03,710
+toxic و corrosive و بسبب حروب و مهيج irritant
+
+117
+00:08:03,710 --> 00:08:10,330
+فبدنا نتعامل مع ال Mercury reaction بحوار طب ايش
+
+118
+00:08:10,330 --> 00:08:11,270
+ال principle اللي
+
+119
+00:08:19,710 --> 00:08:22,810
+التيروسين زي الـmineral reagent حكينا الـmineral
+
+120
+00:08:22,810 --> 00:08:26,670
+reagent هو عبارة عن مكونات نيترك أسد أتشانودري و
+
+121
+00:08:26,670 --> 00:08:30,410
+مالك مجرد ما أضيف الـmineral reagent على التيروسين
+
+122
+00:08:30,410 --> 00:08:35,090
+تيجي أول مكون اللي هو الـnitric acid يعمل نيتراشن
+
+123
+00:08:35,090 --> 00:08:38,930
+للبنزين يعمل نيتراشن لحلقة البنزين الموجودة في
+
+124
+00:08:38,930 --> 00:08:42,620
+التيروسينبعد ما يصيرلها نيتراشن بيجي الـ mercury
+
+125
+00:08:42,620 --> 00:08:47,220
+بيرتبط معها وبيتكون عندي لون أحمر طوبي إذا ان هو
+
+126
+00:08:47,220 --> 00:08:51,640
+تيروسين مع ميلون رياجينت وبيتكون عندي نيتراتي
+
+127
+00:08:51,640 --> 00:08:55,800
+تيروسين ميركاري كومبكس اللي هو تيروسين بيصيرله
+
+128
+00:08:55,800 --> 00:08:59,560
+نيتراشن ومرتبط معه mercury بنسميها نيتراتي تيروسين
+
+129
+00:08:59,560 --> 00:09:05,160
+ميركاري كومبكس شو اللونه بيكون بركره لون أحمر طوبي
+
+130
+00:09:05,160 --> 00:09:08,180
+إذا أن هو positive إذا أن هو التيروسين أو أي
+
+131
+00:09:08,180 --> 00:09:12,530
+بروتين يحتوي على التيروسيننحكي للملاحظة أن كل
+
+132
+00:09:12,530 --> 00:09:16,690
+الفينول اللى بتحتوي كل الفينول كل المركبات اللى
+
+133
+00:09:16,690 --> 00:09:20,530
+عندها فينول ال group اللى حلقة بنزينة مرتبطة ب OH
+
+134
+00:09:20,530 --> 00:09:26,190
+هتعطينا positive مع الفينول ريجال ان احنا حكينا بس
+
+135
+00:09:26,190 --> 00:09:28,570
+طب ايش ال principle
+
+136
+00:09:34,850 --> 00:09:38,270
+طيروسين طيروسين واضيف عليه melon reagent طيروسين
+
+137
+00:09:38,270 --> 00:09:40,950
+زي الـ melon reagent حكينا الـ melon reagent هو
+
+138
+00:09:40,950 --> 00:09:45,790
+عبارة عن مكونات نيترك أسد أتشانواتري و مركز مجرد
+
+139
+00:09:45,790 --> 00:09:49,130
+ما أضيف الـ melon reagent على الطيروسين تيجي اول
+
+140
+00:09:49,130 --> 00:09:52,710
+ايه؟ اول مكون اللي هو الـ nitric acid يعمل نيتراشن
+
+141
+00:09:52,710 --> 00:09:56,560
+للبنزينبيعمل Nitration لحلقة البنزين الموجودة في
+
+142
+00:09:56,560 --> 00:09:59,980
+الـ Tyrosine، بعد ما يصير لها Nitration، بيجي الـ
+
+143
+00:09:59,980 --> 00:10:04,440
+Mercury بيرتبط معها، وبيتكون عندى لون أحمر طوف،
+
+144
+00:10:04,440 --> 00:10:08,280
+اذا ان هو Tyrosine مع ميلونرياجنت، بيتكون عندى
+
+145
+00:10:08,280 --> 00:10:12,860
+Nitrated Tyrosine Mercury Complexاللي هو تيروسين
+
+146
+00:10:12,860 --> 00:10:15,760
+بسيريلو نيتراشن ومرتبط معاه ميركري، بتسميها
+
+147
+00:10:15,760 --> 00:10:19,960
+نيتراتي تيروسين ميركري كومبلاز، شو لونه؟ بيكون
+
+148
+00:10:19,960 --> 00:10:24,560
+فريكري، لون أحمر، طوبي، إذا ان هو positive، إذا ان
+
+149
+00:10:24,560 --> 00:10:28,860
+هو التيروسين أو أي بلوتين يحتوي على التيروسينبحكي
+
+150
+00:10:28,860 --> 00:10:33,420
+للملاحظة أن كل الفينول اللى بتحتوي كل الفينول كل
+
+151
+00:10:33,420 --> 00:10:37,080
+المراكبات اللى عندها فينول group هي حلقة بنزين
+
+152
+00:10:37,080 --> 00:10:41,800
+مرتبطة بهواتف متعطين positive مع الميلونريال أن
+
+153
+00:10:41,800 --> 00:10:45,460
+إحنا حكينا بس من الأحماض الأمنية التيروسين هو
+
+154
+00:10:45,460 --> 00:10:48,400
+الوحيد اللى عنده فينول group عنده حلقة بنزين
+
+155
+00:10:48,400 --> 00:10:52,400
+مرتبطة فيه هواتف لكن هل الطبيعة كلها في المراكبات
+
+156
+00:10:52,400 --> 00:10:56,000
+الكيميائية كلها فش غير التيروسين صح وفي الأحماض
+
+157
+00:10:56,000 --> 00:11:01,350
+الأمنية بسالثانية فيه مركبات تانية عند حلقة بنزين
+
+158
+00:11:01,350 --> 00:11:04,970
+مرتبطة بواعي فلو جيت اشتغلت عليها ال melon test
+
+159
+00:11:04,970 --> 00:11:09,010
+هتعطيني positive طب على فرض انا أعطيتك محلول
+
+160
+00:11:09,010 --> 00:11:12,810
+حكيتلك شوفيلي إيش هذا المحلول يسيغليلي عليها ال
+
+161
+00:11:12,810 --> 00:11:16,630
+melon شوفي تايروسين positive ولا نجده فجيت ان انت
+
+162
+00:11:16,630 --> 00:11:20,330
+اشتغلتيه و أعطاكي positive إذا هو هتتوقع إنه مركب
+
+163
+00:11:20,330 --> 00:11:24,960
+يحتوي على phenolgo تمام؟طب هل هو تيروسين ولا
+
+164
+00:11:24,960 --> 00:11:29,400
+مُركّب تاني؟ كيف بتتأكد هل هو تيروسين أو لا؟ لأن
+
+165
+00:11:29,400 --> 00:11:32,920
+في مُركّبات تانية تحتوي على Phenol Group؟ بشتغل
+
+166
+00:11:32,920 --> 00:11:36,720
+فحص الـNihidrine، مش الـNihidrine أول فحص أخدنا هو
+
+167
+00:11:36,720 --> 00:11:40,980
+مُخصص لـ Free Alpha Amino Group اللي موجودة في كل
+
+168
+00:11:40,980 --> 00:11:45,310
+الأحماض الأمنية، الـAmino Acidأنا هشتغل فحص الـ
+
+169
+00:11:45,310 --> 00:11:49,610
+Ninhydrin لما تشتغلي فحص الـ Ninhydrin إذا أعطاكي
+
+170
+00:11:49,610 --> 00:11:52,810
+positive إذا هو تيروسين لأن حمض أميني يحتوي على
+
+171
+00:11:52,810 --> 00:11:55,830
+free alpha amino group إذا أعطاني negative إذا هو
+
+172
+00:11:55,830 --> 00:12:00,030
+مرنكب تيميائي آخر يحتوي على phenol group غير
+
+173
+00:12:00,030 --> 00:12:00,770
+التيروسين
+
+174
+00:12:04,290 --> 00:12:07,210
+كيف ال procedure؟ خدت وقت إجراء التجربة، بجيب
+
+175
+00:12:07,210 --> 00:12:10,410
+التيور، بحط فيها واحد ميل من ال solution اللي أنا
+
+176
+00:12:10,410 --> 00:12:14,410
+أعطيتها على تلاتة drop من ال melon raisin و بعدين
+
+177
+00:12:14,410 --> 00:12:17,470
+بحطه في ال water bath لمدة خمسة minutes، في ماء
+
+178
+00:12:17,470 --> 00:12:20,430
+مغليه لمدة خمسة minutes، بعدين بطلع، إذا تغير
+
+179
+00:12:20,430 --> 00:12:24,210
+اللون و اتكون عندي break red، إذا هو positive، إذا
+
+180
+00:12:24,210 --> 00:12:27,750
+ما تغيرش اللون، ماعطانيش ال precipitate break red،
+
+181
+00:12:27,750 --> 00:12:29,210
+إذا هو negative
+
+182
+00:12:32,100 --> 00:12:35,780
+الفحص اللي بعد هي اسمه Sakaguchi Test Sakaguchi
+
+183
+00:12:35,780 --> 00:12:39,500
+Test ال objective منه لكشف عن الأحماض الأمنية اللي
+
+184
+00:12:39,500 --> 00:12:44,160
+بتحتوي على Iguanidia Migrum وهو فقط ال Arginine
+
+185
+00:12:44,160 --> 00:12:48,860
+موجودة في Iguanidia Migrum في ال Arginine فحص خاص
+
+186
+00:12:48,860 --> 00:12:53,780
+لل Arginine أنه بيحتوي على Iguanidia Migrumال
+
+187
+00:12:53,780 --> 00:12:56,280
+ساكيا جوشي تيست اناش ناخد لا لبرنسيب اللي هو
+
+188
+00:12:56,280 --> 00:13:00,020
+للطريقة بس انه ال objective هو لل guanidine مجروم
+
+189
+00:13:00,020 --> 00:13:03,360
+اللي موجودة فيه اللي هجنين و ال positive انه
+
+190
+00:13:03,360 --> 00:13:05,320
+يعطيني لون red color
+
+191
+00:13:10,120 --> 00:13:14,200
+الفحص اللي بعد هي اسمه lip sulfite test أو sulfur
+
+192
+00:13:14,200 --> 00:13:18,340
+test ايش ال objective منه؟ للكشف عن ال self
+
+193
+00:13:18,340 --> 00:13:22,520
+-hydrayl group تدور على ال self-hydryl group
+
+194
+00:13:22,520 --> 00:13:25,740
+الموجودة وين ال self-hydryl group؟ في الحمض
+
+195
+00:13:25,740 --> 00:13:29,260
+الأميني cysteine وموجودة كمان في ال cysteine
+
+196
+00:13:29,260 --> 00:13:33,160
+وموجودة في الميثيونين ال self-hydryl group موجودة
+
+197
+00:13:33,160 --> 00:13:38,380
+في ثلاثة، cysteine, cysteine, methionineولكن بتكون
+
+198
+00:13:38,380 --> 00:13:42,960
+في الـ cysteine و الـ cysteine free ال مثيولين
+
+199
+00:13:42,960 --> 00:13:47,640
+بتكون not free بتقدرش تتفاعل لأنه بيكون مربوطة
+
+200
+00:13:47,640 --> 00:13:51,280
+برابطتين carbon من جهة و carbon أخرى من جهة أخرى
+
+201
+00:13:51,280 --> 00:13:55,280
+فغير قادر على كسر الرابط و أنه يتفاعل فهو negative
+
+202
+00:13:55,280 --> 00:13:58,540
+في حالة ال مثيولين و positive في حالة ال cysteine
+
+203
+00:13:58,540 --> 00:14:04,180
+و ال cysteine و أي بروتين يحتوي عليهتمام إذا للـ
+
+204
+00:14:04,180 --> 00:14:06,700
+lead sulphide test أو الـ sulphur test اللي كاشف
+
+205
+00:14:06,700 --> 00:14:10,040
+عن ال sulphohydryl group الموجودة في ال 16 أو ال
+
+206
+00:14:10,040 --> 00:14:14,340
+16 مثيلين نيجاتر لإنها not freeماشي ال principle
+
+207
+00:14:14,340 --> 00:14:18,980
+أني بجيب الحمض الأميني مثلا سيستين أو سيستان بضيف
+
+208
+00:14:18,980 --> 00:14:24,480
+عليها 10% من ال NaOH في وجود حرارة heat بيعطيني
+
+209
+00:14:24,480 --> 00:14:30,440
+Sodium Sulphate بجيب سيستين أو سيستان مع 10% NaOH
+
+210
+00:14:30,440 --> 00:14:34,880
+في وجود حرارة بتكون عندي Sodium Sulphate بضيف
+
+211
+00:14:34,880 --> 00:14:40,060
+عليها 10% Lead Acetate بيعطيني حاجة بتسميها Lead
+
+212
+00:14:40,060 --> 00:14:44,260
+Sulphateالليد سالفايت هذا عبارة عن ال precipitates
+
+213
+00:14:44,260 --> 00:14:50,260
+لونه أسود، 60 أو سيستان، مع 10% النيوهاتش في وجود
+
+214
+00:14:50,260 --> 00:14:55,180
+حرارة، بيعطيني Sodium Sulfate، بضيف عليه 10% ليد
+
+215
+00:14:55,180 --> 00:14:59,360
+أسيتات، بيعطيني Lead Sulfide ب precipitates لونه
+
+216
+00:14:59,360 --> 00:15:03,720
+أسود، يعني positive، يعني بتحتوي على 60 أو سيستان
+
+217
+00:15:07,940 --> 00:15:11,080
+هنا بيوضحنى ان الميثيونين وال سيستين التنتين
+
+218
+00:15:11,080 --> 00:15:14,240
+بيحتوى على sulfur group بس ليش الـ 16 بس هي اللى
+
+219
+00:15:14,240 --> 00:15:17,300
+بتعطينى positive لو دينا ال structure الميثيونين
+
+220
+00:15:17,300 --> 00:15:22,380
+تشوفوا ال S و N مرتبطة في CH2 من جهة و CH3 من جهة
+
+221
+00:15:22,380 --> 00:15:25,880
+فهي مرتبطة من الجهتين و صعب تكسير هذه الرابطة
+
+222
+00:15:26,100 --> 00:15:29,880
+فبنقدرش نكسرها عشان تتفاعل ما يتفاعل فهي not free
+
+223
+00:15:29,880 --> 00:15:33,980
+أما في حالة ال cysteine فهي free هي الأس فوق
+
+224
+00:15:33,980 --> 00:15:37,860
+مرتبطة في H من جهة و free من جهة أخرى تقدر تتفاعل
+
+225
+00:15:37,860 --> 00:15:42,780
+و تحت معادلات لبرنسبل الفحص cysteine مضيف عليه
+
+226
+00:15:42,780 --> 00:15:47,180
+النيوهاتش في وجود حرارة بيعطيني صوديوم سالفايت
+
+227
+00:15:47,180 --> 00:15:52,240
+مضيف عليها lead 10% من lead أسيتات بيعطيني lead
+
+228
+00:15:52,240 --> 00:15:56,600
+سالفايت ال precipitate لونه أسوديكون ال recipe
+
+229
+00:15:56,600 --> 00:16:02,600
+اللي هو هنا اسمهكيف خطوات العمل؟ بحط واحد مل من ال
+
+230
+00:16:02,600 --> 00:16:06,300
+solution أنا أعطيتكيا في تيووب بحط واحد مل منه على
+
+231
+00:16:06,300 --> 00:16:10,480
+واحد مل من ال sodium hydroxide ده نيو هاتش مع خمسة
+
+232
+00:16:10,480 --> 00:16:13,580
+دروب من عشرة فالمية lead acetate بحطهم كلهم مع بعض
+
+233
+00:16:13,580 --> 00:16:17,160
+و بعمل math بعدين بحطه في ال water bath لمدة خمسة
+
+234
+00:16:17,160 --> 00:16:21,020
+منه بطلعه من ال water bath إذا صار لونه أسود فيه
+
+235
+00:16:21,020 --> 00:16:24,400
+نراسب أسود is a positive يحتوي على ال system إذا
+
+236
+00:16:24,400 --> 00:16:28,940
+ما اتغيرش اللون is a negative ليحتوي على ال system
+
diff --git a/PL9fwy3NUQKwZ1Q7zOg1ObgufPMjRI3WY1/VOnoEDYqP7I_postprocess.srt b/PL9fwy3NUQKwZ1Q7zOg1ObgufPMjRI3WY1/VOnoEDYqP7I_postprocess.srt
new file mode 100644
index 0000000000000000000000000000000000000000..2c39f6777633a96e52b33e56b6e7918cdd4c8e3e
--- /dev/null
+++ b/PL9fwy3NUQKwZ1Q7zOg1ObgufPMjRI3WY1/VOnoEDYqP7I_postprocess.srt
@@ -0,0 +1,2104 @@
+1
+00:00:00,770 --> 00:00:04,710
+اليوم ان شاء الله بدنا نحكي في موضوع جديد اللي هو
+
+2
+00:00:04,710 --> 00:00:09,590
+موضوع الدهون بدنا نحكي عن ال libid عن الدهون نتعرف
+
+3
+00:00:09,590 --> 00:00:16,030
+عليها وعلى فحوصات للكشف عن الدهون في البداية بدنا
+
+4
+00:00:16,030 --> 00:00:21,550
+نحكي تعريف ال libid ال libid هي group of naturally
+
+5
+00:00:21,550 --> 00:00:25,030
+occurring substance consisting of higher fatty
+
+6
+00:00:25,030 --> 00:00:28,710
+acid وهي naturally occurring substance أو compound
+
+7
+00:00:30,320 --> 00:00:35,920
+مركبات أو مجموعة من المكو .. من المركبات تتكون من
+
+8
+00:00:35,920 --> 00:00:41,060
+نسبة عالية من الأحماض الدهنية، لذان الدهون هي
+
+9
+00:00:41,060 --> 00:00:46,760
+عبارة عن مركبات تحتوي على نسبة عالية من الأحماض
+
+10
+00:00:46,760 --> 00:00:50,440
+الدهنية، من ال fatty acid، من الأحماض الدهنية، وهي
+
+11
+00:00:50,440 --> 00:00:53,200
+in the generally occurring substance هي مواد
+
+12
+00:00:53,200 --> 00:00:59,020
+موجودة طبيعيا في طعامنا اليومOrganic substance
+
+13
+00:00:59,020 --> 00:01:02,140
+relatively insoluble in water but soluble in
+
+14
+00:01:02,140 --> 00:01:05,780
+organic solvent بيحكي لإن اللي بتطبيعتها هي
+
+15
+00:01:05,780 --> 00:01:11,780
+organic هي مواد عضوية هي non-polar هي مواد عضوية
+
+16
+00:01:11,780 --> 00:01:16,580
+non-polarلا تدوب في الماء مدامها non-polar إذا مش
+
+17
+00:01:16,580 --> 00:01:20,860
+هتدوب في الماء في الماء وين هتدوب؟ بتدوب بيحكيلي
+
+18
+00:01:20,860 --> 00:01:25,400
+في ال organic solvent في اللي بيشبهها المبدأ like,
+
+19
+00:01:25,500 --> 00:01:30,580
+dissolve like المتشابهات دوتيب بعضها فهي بتدوب في
+
+20
+00:01:30,580 --> 00:01:34,280
+المواد العضوية اللي زيها زي مين مواد عضوية بتدوب
+
+21
+00:01:34,280 --> 00:01:39,430
+فيها ال lipid؟ زي chloroform, ether, benzeneعلى
+
+22
+00:01:39,430 --> 00:01:45,250
+مبدأ like dissolve like إن المتشابهات تديب بعضها
+
+23
+00:01:45,250 --> 00:01:50,790
+function of libid لو جينا نتعرف على وظائف اللبد
+
+24
+00:01:50,790 --> 00:01:55,210
+أول وظيفة حكينا عنها في أول معمل إنها storage form
+
+25
+00:01:55,210 --> 00:02:00,510
+of energy هي مخزن الطاقة ال libid تعتبر مخزن
+
+26
+00:02:00,510 --> 00:02:04,510
+الطاقة الوظيفة التانية هي structural component of
+
+27
+00:02:04,510 --> 00:02:09,460
+cell membraneهي بتدخل في تركيب ال cell membrane في
+
+28
+00:02:09,460 --> 00:02:14,860
+تركيب الغلاف الخلوي الجدار الخلوي بتدخل فيه تركيبه
+
+29
+00:02:14,860 --> 00:02:19,140
+اللبن تلاتة هي precursor of many steroid hormone
+
+30
+00:02:19,140 --> 00:02:25,380
+as estrogen و vitamin D هي اللبن الأساسية في تركيب
+
+31
+00:02:25,380 --> 00:02:29,000
+الهرمونات الاسترويدية اللي هي نفسها ال sex hormone
+
+32
+00:02:29,000 --> 00:02:33,600
+زي الاستروجين و vitamin D اللي برضه هي بتدخل فيه
+
+33
+00:02:33,600 --> 00:02:39,160
+تركيبهprotection of internal organs تعمل على حماية
+
+34
+00:02:39,160 --> 00:02:44,180
+الأعضاء الداخلية الدهون تعمل على حماية الأعضاء
+
+35
+00:02:44,180 --> 00:02:44,980
+الداخلية
+
+36
+00:02:47,330 --> 00:02:51,990
+هنا فيه إنه رسمة ل cell membrane بدي واضحني إنه
+
+37
+00:02:51,990 --> 00:02:54,730
+أغلب مكونات ال cell membrane هي عبارة عن lipid
+
+38
+00:02:54,730 --> 00:02:58,210
+يعني outside of cell اللي خارج الخلية فيها
+
+39
+00:02:58,210 --> 00:03:01,550
+نسفينجومايليين هذا lipid ال glycolipid برضه lipid
+
+40
+00:03:01,550 --> 00:03:05,670
+و ال phosphatigolين فيها ناكلسترول كل هذه المكونات
+
+41
+00:03:05,670 --> 00:03:09,210
+عبارة عن lipid فأغلب مكونات ال cell membrane هي
+
+42
+00:03:09,210 --> 00:03:14,290
+عبارة عن lipid فهو التركيبة الأساسية ل ال cell
+
+43
+00:03:14,290 --> 00:03:14,830
+membrane
+
+44
+00:03:17,980 --> 00:03:22,760
+أنواع الدهون type of lipid إيش أنواع الدهون الدهون
+
+45
+00:03:22,760 --> 00:03:28,360
+قُسمت بشكل عام إلى lipid with fatty acid و lipid
+
+46
+00:03:28,360 --> 00:03:34,060
+without fatty acid يعني الدهون بشكل عام قُسمت إلى
+
+47
+00:03:34,060 --> 00:03:39,620
+قسمين دهون تحتوي على fatty acid ودهون لا تحتوي على
+
+48
+00:03:39,620 --> 00:03:42,940
+fatty acid lipid with fatty acid و lipid without
+
+49
+00:03:42,940 --> 00:03:46,680
+fatty acidاللي with fatty acid تحتوي على fatty
+
+50
+00:03:46,680 --> 00:03:51,280
+acid من الامتل عليها ال waxes، fat and oil، الذبدة
+
+51
+00:03:51,280 --> 00:03:55,640
+ويئ الزيت، phospholipid، sphingolipid، lipid
+
+52
+00:03:55,640 --> 00:03:59,900
+without fatty acid زي الستيرون، هاي التقسيمة بشكل
+
+53
+00:03:59,900 --> 00:04:04,790
+عام وهالجيتها نبدأ نفرع منهالقيت احنا حاكي هنا a
+
+54
+00:04:04,790 --> 00:04:08,350
+classification هنا of lipid نبدأ نفعلها اللبد بشكل
+
+55
+00:04:08,350 --> 00:04:12,490
+عام تنقسم إلى lipid with fatty acid تحتوي على
+
+56
+00:04:12,490 --> 00:04:16,330
+fatty acid و lipid without fatty acid ده تحتوي على
+
+57
+00:04:16,330 --> 00:04:19,870
+fatty acid ناخد الجزء الأول اللي هي اللبد with
+
+58
+00:04:19,870 --> 00:04:24,770
+fatty acid اللي بتحتوي على fatty acid اللبد اللتي
+
+59
+00:04:24,770 --> 00:04:28,470
+تحتوي على fatty acid تنقسم إلى قسمين في منها
+
+60
+00:04:28,470 --> 00:04:33,780
+simple lipid و في منها conjugate في simpleوفي
+
+61
+00:04:33,780 --> 00:04:36,920
+conjugate هاي ال libid with fatty acid تنقسم إلى
+
+62
+00:04:36,920 --> 00:04:41,260
+simple و conjugate نحكي عن ال simple بيحكي إنه ال
+
+63
+00:04:41,260 --> 00:04:45,320
+simple libid تركيبتها الأساسية يبارح عن fatty acid
+
+64
+00:04:45,320 --> 00:04:50,340
+مع كحول إذا كان بس عبارة عن fatty acid و كحول
+
+65
+00:04:50,340 --> 00:04:53,840
+تركيب الأساسية إليه fatty acid و كحول بنسميه
+
+66
+00:04:53,840 --> 00:04:58,320
+simple libid بيحكي إنه الكحول هاد ممكن يكون
+
+67
+00:04:58,320 --> 00:05:03,780
+glycerol أو كحول نوع تاني غير ال glycerolإذا فاتي
+
+68
+00:05:03,780 --> 00:05:07,980
+أسد متصلة بكحول بسميها سم باللبن طب الكحول هاد إما
+
+69
+00:05:07,980 --> 00:05:11,680
+بيكون ممكن يكون نوعه إيش هيكون نوعه ممكن يكون
+
+70
+00:05:11,680 --> 00:05:16,240
+glycerol أو حاجة تانية كحول نوع تاني غير ال
+
+71
+00:05:16,240 --> 00:05:21,160
+glycerol كيف بيرتبط الفاتي أسد مع الكحول ب Easter
+
+72
+00:05:21,160 --> 00:05:24,560
+bond by Easter bond اللي هي الرابطة الدهنية هذا
+
+73
+00:05:24,560 --> 00:05:28,980
+اسم الرابطة الدهنية بيرتبط الفاتي أسد مع الكحول
+
+74
+00:05:28,980 --> 00:05:34,530
+برابطة دهنيةلو الكحول هات كان glycerol الناتج من
+
+75
+00:05:34,530 --> 00:05:38,970
+اللي بدهات بسميه اما oil او fat يعني اذا fatty
+
+76
+00:05:38,970 --> 00:05:44,110
+acid مرتبط معها glycerol بسميها oil هينتج عنها oil
+
+77
+00:05:44,110 --> 00:05:48,290
+او fat ال oil هو عبارة عن ال tri-glyceride rich in
+
+78
+00:05:48,290 --> 00:05:51,830
+unsaturated fatty acid و بيكون liquid at room
+
+79
+00:05:51,830 --> 00:05:55,210
+temperature الزيت ال oil هو عبارة عن ال tri
+
+80
+00:05:55,210 --> 00:05:58,630
+-glyceride tri معناها تلاتة يعني تلاتة fatty acid
+
+81
+00:05:58,900 --> 00:06:03,120
+مرتبطين مع glycerol مع الكحول try معناها تلاتة
+
+82
+00:06:03,120 --> 00:06:07,420
+fatty acid مع glycerol الكحول هذا ال oil بيكون
+
+83
+00:06:07,420 --> 00:06:11,460
+بيحكي ليهو unsaturated fatty acid يحتوي على
+
+84
+00:06:11,460 --> 00:06:15,880
+unsaturated fatty acid إيش يعني unsaturated يعني
+
+85
+00:06:15,880 --> 00:06:21,940
+أحماض دهنية غير مشبعة يعني تحتوي على رابطة ثنائية
+
+86
+00:06:21,940 --> 00:06:27,670
+فأكتر وال oil هات بيكون liquid قوامه بيكون سائلأما
+
+87
+00:06:27,670 --> 00:06:31,290
+الفات فهو برضه triglyceride تلاتة fatty acid مع
+
+88
+00:06:31,290 --> 00:06:35,750
+glycerol ولكن هو غني بال saturated fatty acid
+
+89
+00:06:35,750 --> 00:06:40,630
+أحماض دهنية مشبعة وبيكون على درجة حرارة الغرفة
+
+90
+00:06:40,630 --> 00:06:43,810
+بيكون عبارة عن solid أو semi solid بيكون صلب أو
+
+91
+00:06:43,810 --> 00:06:50,530
+شبه الصلب هذه الفات الزبدة طيب لو الكحول كانت
+
+92
+00:06:50,530 --> 00:06:53,610
+حاجتان غير ال glycerol كان عبارة عن fatty acid
+
+93
+00:06:53,610 --> 00:06:57,850
+أحمض دهنية مرتبط معها كحول غير ال glycerolبينتج
+
+94
+00:06:57,850 --> 00:07:02,690
+عندي حاجة اسمها waxes اللي هينتج عندي حاجة اسمها
+
+95
+00:07:02,690 --> 00:07:07,150
+waxes اذا اول نوع من احنا اللي بتقسمها بشكل عام
+
+96
+00:07:07,150 --> 00:07:10,010
+الى with fatty acid و without fatty acid ال with
+
+97
+00:07:10,010 --> 00:07:13,590
+fatty acid في منها simple و في منها conjugate ال
+
+98
+00:07:13,590 --> 00:07:17,010
+simple اللي هادا بيكون عبارة عن fatty acid مع كحول
+
+99
+00:07:17,010 --> 00:07:21,530
+اذا الكحول كان glycerol بيعطيني oil و fat اذا حاجة
+
+100
+00:07:21,530 --> 00:07:25,170
+تانية غير ال glycerol بيعطيني حاجة اسمها waxes
+
+101
+00:07:28,030 --> 00:07:30,930
+القسم التاني من اللبد with fatty acid هي ال
+
+102
+00:07:30,930 --> 00:07:36,930
+conjugate لبن conjugate لبن هي عبارة عن اللبد في
+
+103
+00:07:36,930 --> 00:07:41,350
+تركيبته بيكون بيحتوي على fatty acid مع كحول مع
+
+104
+00:07:41,350 --> 00:07:45,390
+additional group مع group كمان زيادة بيكون عبارة
+
+105
+00:07:45,390 --> 00:07:50,410
+عن تركيبته fatty acid أحمد دهنية مرتبطة مع الكحول
+
+106
+00:07:50,410 --> 00:07:54,570
+ومرتبط عليها كمان group زيادة additional group
+
+107
+00:07:55,420 --> 00:07:59,700
+fatty acid مع كحول مع additional group مع group
+
+108
+00:07:59,700 --> 00:08:07,500
+تاني لو الكحول لو مع additional group sorry مع
+
+109
+00:08:07,500 --> 00:08:10,780
+additional group مع group تاني الجد بناء على ال
+
+110
+00:08:10,780 --> 00:08:14,740
+group اللي بتتصل في هذا ال lipid بسميه lipid فإذا
+
+111
+00:08:14,740 --> 00:08:17,240
+ال group اللي اتصلت معاه كانت phosphate group
+
+112
+00:08:17,240 --> 00:08:21,400
+بسميه phospholipid phospholipid هو عبارة عن
+
+113
+00:08:21,400 --> 00:08:25,750
+conjugate lipid يحتوي على fatty acidمع كحول مع
+
+114
+00:08:25,750 --> 00:08:31,690
+phosphoric acid الـ glycolipid هو عبارة عن لبد
+
+115
+00:08:31,690 --> 00:08:36,010
+يحتوي .. كنجقة لبد يحتوي على fatty acid مع كحول مع
+
+116
+00:08:36,010 --> 00:08:39,230
+carbohydrate الـ glyco جاية من carbohydrate اللي
+
+117
+00:08:39,230 --> 00:08:44,930
+هي السكة الـ sulfolipid هو عبارة عن fatty acid مع
+
+118
+00:08:44,930 --> 00:08:50,490
+كحول مع sulfate groupفوسفو لبد فوسفات جروب بتكون
+
+119
+00:08:50,490 --> 00:08:54,930
+متصلة فيهم الجلايكو بتكون carbohydrate صلفو بيكون
+
+120
+00:08:54,930 --> 00:08:59,790
+صلفات جروب ليبوبروتين بيكون متصل معها البروتين
+
+121
+00:08:59,790 --> 00:09:06,090
+عبارة عن fatty acid مع كحول و البروتين متصلة فيها
+
+122
+00:09:06,090 --> 00:09:10,290
+لذا ال conjugate لبد هو عبارة عن fatty acid مع
+
+123
+00:09:10,290 --> 00:09:14,830
+كحول مع جروب كمان مضافة عليهاهذه ال group هي اللي
+
+124
+00:09:14,830 --> 00:09:17,630
+اعطته الاسم إذا هي phosphate group بسميها
+
+125
+00:09:17,630 --> 00:09:21,710
+phospholipin إذا carbohydrate بسميها glycolipin
+
+126
+00:09:21,710 --> 00:09:25,510
+إذا sulfate بسميها sulfolipin وإذا ال protein
+
+127
+00:09:25,510 --> 00:09:33,150
+بسميها lipoprotein هنا عندنا مخطط بيوضحلي تقسيمة
+
+128
+00:09:33,150 --> 00:09:36,330
+انه كيف بتقسم اللبن هو بشكل عام احنا هذه ال
+
+129
+00:09:36,330 --> 00:09:39,730
+structures مش مطلوبة مننا لكن بشكل عام نعرف ان
+
+130
+00:09:39,730 --> 00:09:44,380
+اللبن مقسم إلى with fatty acidwithout fatty acid
+
+131
+00:09:44,380 --> 00:09:47,640
+ال with fatty acid اللي اتفرعنا فيها في منها
+
+132
+00:09:47,640 --> 00:09:51,680
+simple و في منها conjugate ال simple هي عبارة عن
+
+133
+00:09:51,680 --> 00:09:55,700
+fatty acid مع كحول و الكحول هذا إما يكون glycerol
+
+134
+00:09:55,700 --> 00:09:59,100
+أو حاجة تانية غير ال glycerol إذا كان glycerol
+
+135
+00:09:59,100 --> 00:10:02,520
+بيعطيني fat و oilإذا حاجة تانية غير ال glycerol
+
+136
+00:10:02,520 --> 00:10:07,500
+بيعطيني waxes، waxes لبد أما الكنجوجات لبد هي
+
+137
+00:10:07,500 --> 00:10:11,720
+عبارة عن fatty acid مع كحول، مع other group، مع
+
+138
+00:10:11,720 --> 00:10:16,980
+group تانية متصلة فيهاو بناء على هاي ال group
+
+139
+00:10:16,980 --> 00:10:19,540
+نسميها phospholipid يعني phosphatic group
+
+140
+00:10:19,540 --> 00:10:23,240
+glycolipid يعني carbohydrate sulfolipid يعني
+
+141
+00:10:23,240 --> 00:10:28,340
+sulfatic group ليبوبروتين يعني متصل فيها بروتين ال
+
+142
+00:10:28,340 --> 00:10:31,660
+without fatty acid، lipid without fatty acid مش
+
+143
+00:10:31,660 --> 00:10:35,020
+متصل فيها fatty acid هي هذه الجزء اللي تحت هنا
+
+144
+00:10:35,020 --> 00:10:38,560
+الجزء
+
+145
+00:10:38,560 --> 00:10:43,200
+اللي تحت ماد لطويل و نازل لتحت مكتوب عندها steroid
+
+146
+00:10:45,230 --> 00:10:48,590
+مكتوب بـ steroids فاللبد الـ without fatty acid
+
+147
+00:10:48,590 --> 00:10:53,510
+نسميها steroids تحتوي على steroid nucleus تحتوي
+
+148
+00:10:53,510 --> 00:10:57,910
+على steroid nucleus من الأمثلة على اللبد اللتي لا
+
+149
+00:10:57,910 --> 00:11:02,170
+تحتوي fatty acid تحتوي على steroid nucleus زي
+
+150
+00:11:02,170 --> 00:11:08,750
+كلسترول و bile salt و steroid hormone كلسترول و
+
+151
+00:11:08,750 --> 00:11:13,010
+bile salt و steroid hormone هدولة لبد without
+
+152
+00:11:13,010 --> 00:11:18,390
+fatty acidطب اليوم الشغل العملي هو عبارة عن
+
+153
+00:11:18,390 --> 00:11:27,330
+qualitative test for لبن لنعمل فحوصات جودة
+
+154
+00:11:27,330 --> 00:11:32,440
+و نوعية للكشف عن اللبن فحوصات qualitativeيعني جودة
+
+155
+00:11:32,440 --> 00:11:36,780
+و نوعية زي فحوصات الكشف عن الأحمد الأمنية فهنا
+
+156
+00:11:36,780 --> 00:11:40,380
+مجموعة فحوصات ممكن يتغير اللون أو مايتغيرش اللون
+
+157
+00:11:40,380 --> 00:11:46,600
+ونشوف فحوصات للكشف عن ال lipids زي مين أول فحص
+
+158
+00:11:46,600 --> 00:11:49,660
+هنتعرف عليه هو solubility in polar and nonpolar
+
+159
+00:11:49,660 --> 00:11:54,600
+solvent فهنا فحص اسمه emulsification test اللي هو
+
+160
+00:11:54,600 --> 00:11:58,540
+الاستحلاب فهنا فحص ال oxidation of unsaturated
+
+161
+00:11:58,540 --> 00:12:03,480
+fatty acid أكسداللـ unsaturated fatty acid ويعني
+
+162
+00:12:03,480 --> 00:12:07,140
+فحص qualitative analysis of detection in natural
+
+163
+00:12:07,140 --> 00:12:12,400
+product اللي هو فحص اسمه سالكا ويسكي reaction هنجي
+
+164
+00:12:12,400 --> 00:12:17,640
+نتعرف على كل فحص على حدة أول فحص هو solubility in
+
+165
+00:12:17,640 --> 00:12:22,200
+polar and nonpolar solvent solubility in polar and
+
+166
+00:12:22,200 --> 00:12:27,570
+nonpolar يعني بدي أجيب اللي بدهونو بدي أدوبه في
+
+167
+00:12:27,570 --> 00:12:32,650
+مواد .. بدي أجيبه و أحطه في مواد عضوية و غير عضوية
+
+168
+00:12:32,650 --> 00:12:36,250
+و أشوف وين يدوب يعني انت جيبله مواد polar و non
+
+169
+00:12:36,250 --> 00:12:39,850
+-polar حاجات اللي بتحكي ان هو non-polar بيدوب في
+
+170
+00:12:39,850 --> 00:12:43,310
+ال non-polar فإذا جبتله non-polar بيدوب فيه إذا
+
+171
+00:12:43,310 --> 00:12:47,370
+جبتله polar مش هيدوب فيه احنا في هذا الفحص بدنا
+
+172
+00:12:47,370 --> 00:12:51,990
+نجيب مي نجيب chloroform نجيب ether نجيب إيثانوانو
+
+173
+00:12:51,990 --> 00:12:55,630
+ندوب اللبد فيهم الزيت مثلا نجيب زيت ندوبه في الماء
+
+174
+00:12:55,630 --> 00:12:59,810
+نشوف انه بدوب ولا بدوبش بعدين بدنا نجيب الزيت و
+
+175
+00:12:59,810 --> 00:13:05,270
+ندوبه في ايه نشوف هل هيدوب ولا مش هيدوب بعدين نجيب
+
+176
+00:13:05,270 --> 00:13:09,530
+كلوروفارب و ندوبه فيه نشوف هل اللبد هيدوب ولا مش
+
+177
+00:13:09,530 --> 00:13:14,790
+هيدوب هنلاحظ ان اللبد بدوب فيهم كله تم هذا ال
+
+178
+00:13:14,790 --> 00:13:19,010
+distill water لأنه عبارة عن boiler و اللبد non
+
+179
+00:13:19,010 --> 00:13:25,110
+boiler فبيدوبش فيهفمبدأ عمل الفحص الـ principle هو
+
+180
+00:13:25,110 --> 00:13:29,650
+على أي مبدأ بناء على إيش هيدوب اللبط في الماء،
+
+181
+00:13:29,650 --> 00:13:33,150
+هيدوب في المركبة العضوية ومش هيدوب في الماء، بناء
+
+182
+00:13:33,150 --> 00:13:37,510
+على أي مبدأ، حكينا بناء على مبدأ like, dissolve,
+
+183
+00:13:37,870 --> 00:13:42,350
+likeلذن ال principle هو like dissolve like ال
+
+184
+00:13:42,350 --> 00:13:45,290
+objective من هذا الفحص أنه أنا بدي أكشف عن
+
+185
+00:13:45,290 --> 00:13:48,650
+solubility of lipids in water and organic solvent
+
+186
+00:13:48,650 --> 00:13:53,330
+اللي هي مادة ذوبانية الدهون في المي و في المديبات
+
+187
+00:13:53,330 --> 00:13:57,950
+العضويةالصليبوليتي بتعتمد على ال polarity
+
+188
+00:13:57,950 --> 00:14:01,770
+الدوبانية بتعتمد على القطمية طيب ايش ال reagent
+
+189
+00:14:01,770 --> 00:14:05,570
+اللى بدنا نستخدمه في الفحص هاد؟ بدنا نستخدم دهون،
+
+190
+00:14:05,570 --> 00:14:08,450
+بدنا نستخدم زيت الزتون و الفات اللى هى ال butter
+
+191
+00:14:08,450 --> 00:14:12,310
+أو الفات اللى هى الزبدة المواد اللى بدنا ندوب فيها
+
+192
+00:14:12,310 --> 00:14:17,470
+ال solvent هي بدنا نستخدم إيثانول و chloroform و
+
+193
+00:14:17,470 --> 00:14:23,470
+ether و waterكيف بدي أشتغل الفحص؟ بدي أجيب أربع
+
+194
+00:14:23,470 --> 00:14:27,350
+تيوبات واحدة تشتغل فيها ديستل واتر واحدة إيثر
+
+195
+00:14:27,350 --> 00:14:31,990
+واحدة كلوروفور واحدة إيثانور تمام؟ بدي أجيب لتيوب
+
+196
+00:14:31,990 --> 00:14:35,670
+الأول تاعة الديستل واتر بدي أحط فيها drop oil drop
+
+197
+00:14:35,670 --> 00:14:40,590
+من الزيت على واحد مل من الديستل واتر بدي أجيب
+
+198
+00:14:40,590 --> 00:14:45,000
+لتيوب الأولأحط فيها drop من ال oil نقطة من الزيت
+
+199
+00:14:45,000 --> 00:14:48,180
+على واحد من ال distill water و أعمل mix أشوف هل
+
+200
+00:14:48,180 --> 00:14:52,660
+هيكون دائب ولا غير دائب soluble او insoluble ال
+
+201
+00:14:52,660 --> 00:14:56,320
+cube التانية بدي أحط عليها drop من ال oil على واحد
+
+202
+00:14:56,320 --> 00:15:01,060
+من ال ether بدي أحط منها drop من الزيت على واحد من
+
+203
+00:15:01,060 --> 00:15:05,330
+ال ether و أشوف بيدوب ولا بيدوبشهجيب ال tube
+
+204
+00:15:05,330 --> 00:15:09,290
+التالت فيها chlorofar حط واحد drop من الزيت على
+
+205
+00:15:09,290 --> 00:15:13,510
+واحد من ال chlorofar هشوف بيدوب ولا لأ، ال tube
+
+206
+00:15:13,510 --> 00:15:16,850
+الرابع ده حط فيها drop من ال oil على واحد من ال
+
+207
+00:15:16,850 --> 00:15:21,960
+ethanol، هشوف بيدوب ولا بيدوبشنفس الخطوات بدي أجيب
+
+208
+00:15:21,960 --> 00:15:25,580
+أربعة يوبات تانية و أعمل فيها نفس الخطوات اللي هي
+
+209
+00:15:25,580 --> 00:15:28,480
+واحدة distil water واحدة ether واحدة chloroform
+
+210
+00:15:28,480 --> 00:15:32,020
+واحدة ethanol بس بدل الزيت بدي أحط butter بدي أحط
+
+211
+00:15:32,020 --> 00:15:35,240
+زبدة زبدة مع distil water شوف بيدوب ولا بيدوبش
+
+212
+00:15:35,240 --> 00:15:39,200
+ether مع زبدة شوف بيدوب ولا بيدوبش chloroform مع
+
+213
+00:15:39,200 --> 00:15:42,880
+زبدة شوف بيدوب ولا بيدوبش ethanol مع زبدة هل بيدوب
+
+214
+00:15:42,880 --> 00:15:47,380
+ولا مابيدوبش ده شوف هدول بيدوبوا ولا مابيدوبوامش
+
+215
+00:15:47,380 --> 00:15:52,060
+بنتوقع؟ بنتوقع إنه يدوب في كل ما عدا الـ distilled
+
+216
+00:15:52,060 --> 00:15:58,640
+water مش هيدوب في الـ distilled water هدر بالنسبة
+
+217
+00:15:58,640 --> 00:16:02,880
+للفحص الأول فحص التاني هو اسمه ال emulsification
+
+218
+00:16:02,880 --> 00:16:08,440
+emulsification معناه استحلامemulsification معناها
+
+219
+00:16:08,440 --> 00:16:14,780
+استحلاب إيش يعني استحلاب؟ يعني جعل مادتين ما
+
+220
+00:16:14,780 --> 00:16:18,540
+بيدوبوا ببعض طبيعي اللي هي دوبوا ببعض بدي أخليهم
+
+221
+00:16:18,540 --> 00:16:24,480
+بيدوبوا ببعض بمساعدة emulsifying agent بمساعدة
+
+222
+00:16:24,480 --> 00:16:29,270
+عامل مستحلبإذا ال emulsification أنه أنا بدي أخلي
+
+223
+00:16:29,270 --> 00:16:33,550
+مادتين هم أصلا ما بيدوبوا ببعض بدي أخليهم يدوبوا
+
+224
+00:16:33,550 --> 00:16:39,210
+بواسطة عامل مستحلب بمساعدة عامل مستحلب زي مين
+
+225
+00:16:39,210 --> 00:16:42,650
+مادتين بدوبوش ببعض مثلا الزيت مع الميه بدوبوش ببعض
+
+226
+00:16:42,650 --> 00:16:47,490
+أنا بدي أجيب عامل مستحلب بيساعد على أنه الزيت يدوب
+
+227
+00:16:47,490 --> 00:16:52,150
+في الميهزي مثلا هلجيت لو عندنا مجلة المجلة هي هاد
+
+228
+00:16:52,150 --> 00:16:56,150
+فيه زيت و أنت تجيتي تجليه، تخسليه، لو حطيت الميه و
+
+229
+00:16:56,150 --> 00:17:00,890
+خلاص، هالخلاص هينظف و انه هيروح الزيت منه؟ لأ،
+
+230
+00:17:00,890 --> 00:17:04,710
+بتحس انه الزيت يتكوم زي قطرات في هذا المجلة، انت
+
+231
+00:17:04,710 --> 00:17:08,010
+شو بتعمل عشان تشيلي هذا الزيت و تنظفيه خالص؟ بتحطي
+
+232
+00:17:08,010 --> 00:17:12,830
+صابونالصابون بيساعد على إنه يدوب الزيت في المي و
+
+233
+00:17:12,830 --> 00:17:16,470
+يشيل الزيت من المجلة كان جابل الزيت مش راضي يروح
+
+234
+00:17:16,470 --> 00:17:19,850
+بالمي لما حطيتي صابونة الصابونة ساعدت فهنا
+
+235
+00:17:19,850 --> 00:17:24,830
+الصابونة بسميها كإنها emulsifying agent عامل
+
+236
+00:17:24,830 --> 00:17:31,580
+مستحلب ساعد على دوبان الزيت في المي و إنه يختفيمش
+
+237
+00:17:31,580 --> 00:17:34,480
+بيحكيلي أن ال emulsion هو mixture of two or more
+
+238
+00:17:34,480 --> 00:17:38,100
+liquids are normally immiscible، ال emulsion هو
+
+239
+00:17:38,100 --> 00:17:42,520
+عبارة عن خليت من مادتين أو أكتر طبيعيين ما بيدوبوا
+
+240
+00:17:42,520 --> 00:17:47,780
+بالمي زي زيت مع مي و بيدوبوش في بعض طبيعيين،
+
+241
+00:17:47,780 --> 00:17:52,840
+هبسميه emulsionهلجت ال bile salt هيش هي ال bile
+
+242
+00:17:52,840 --> 00:17:56,760
+salt؟ ال bile salt هي ملح العصارة السفراوية بتنتج
+
+243
+00:17:56,760 --> 00:18:01,060
+من الكوليسترول تصنع من الكوليسترول وتخزن في الجالب
+
+244
+00:18:01,060 --> 00:18:08,260
+لدر في المثانا في المرارة تخزن في المرارة لما ان
+
+245
+00:18:08,260 --> 00:18:11,500
+احنا الجسم الحين في جسم الإنسان أغلب مكوناته ماي
+
+246
+00:18:11,500 --> 00:18:14,680
+طب اللي الإنسان لما ياكل واجب الدهون كيف هيبدأ
+
+247
+00:18:14,680 --> 00:18:18,570
+تدوب و تمشي في جسم الإنسان اللي أغلبه ماي؟بحكي إنه
+
+248
+00:18:18,570 --> 00:18:22,290
+إحنا في جسم الإنسان when you eat فات لما تاكلي
+
+249
+00:18:22,290 --> 00:18:26,370
+وجبة دهون، في عندنا ال bile salt بتشتغل في الجسم
+
+250
+00:18:26,370 --> 00:18:30,670
+كصابونة، زي صابونة كأنها، بتدوب ال bile salt و
+
+251
+00:18:30,670 --> 00:18:34,910
+بتخليها ماشية في الجسم بسهولة بين الماءكيف؟ ال
+
+252
+00:18:34,910 --> 00:18:38,590
+bile salt هاي .. هاي الأصفر اللي تحت أصفر ويلها
+
+253
+00:18:38,590 --> 00:18:42,850
+طرف أخضر مكتوب عليها أنها bile salt إيش هاي الطرف
+
+254
+00:18:42,850 --> 00:18:46,810
+الأخضر؟ هاي bile salt إلها جزءين جزء بكون .. بكون
+
+255
+00:18:46,810 --> 00:18:49,550
+منها hydrophilic اللي هو الأخضر الجزء الأخضر
+
+256
+00:18:49,550 --> 00:18:52,570
+hydrophilic والجزء الأصفر بيكون عبارة عن
+
+257
+00:18:52,570 --> 00:18:57,410
+hydrophobic جزء بيكون منها محب للماء وجزء بيكون
+
+258
+00:18:57,410 --> 00:19:01,390
+كاره للماءكيف تيجي تعمل هاي ال bile salt؟ بتيجي
+
+259
+00:19:01,390 --> 00:19:04,310
+على وجبة الدهون اللي عندها، هاي الزرقة الكبيرة
+
+260
+00:19:04,310 --> 00:19:09,710
+بتحوت عليها من جميع الاتجاهات بحيث أنه جزء الكاره
+
+261
+00:19:09,710 --> 00:19:14,770
+للماء يكون للداخل منها والجزء اللي بيحب الماء يكون
+
+262
+00:19:14,770 --> 00:19:19,790
+للخارج فالحين هي كأنها كورة ماشية في الجسم من جوا
+
+263
+00:19:19,790 --> 00:19:24,450
+بتدوبش في الماء لكن من برا محبة للماء فمهما تمشي
+
+264
+00:19:24,450 --> 00:19:28,750
+في الجسم هتبين كأنها دائبة في الماءفالدهون كيف
+
+265
+00:19:28,750 --> 00:19:32,850
+بتمشي في الجسم عن طريق ال bile salt اللي بتعمل
+
+266
+00:19:32,850 --> 00:19:38,310
+كسبونة بتحوت عليها من الداخل بجزء مكاره للماء
+
+267
+00:19:38,310 --> 00:19:46,910
+هيدروفوبك ومن الخارج بجزء هيدروفليك محب للماء طيب
+
+268
+00:19:46,910 --> 00:19:50,130
+هلجيت ال emulsification اللي هو الاستحلاب فيه منه
+
+269
+00:19:50,130 --> 00:19:53,250
+نوعين ننزل تحت على آخر ال slide في emulsification
+
+270
+00:19:53,250 --> 00:19:57,440
+type أنواع الاستحلابفي منه permanent وفي منه
+
+271
+00:19:57,440 --> 00:20:01,520
+temporary permanent يعني بيكون استحلاق بشكل دائم
+
+272
+00:20:01,520 --> 00:20:06,040
+يعني الزيت مع مي ودائبي بشكل دائم مهما أسيب التيوب
+
+273
+00:20:06,040 --> 00:20:09,800
+أرجها و أرجع أسيبها بخلق بلاجيهم دايما منتشرين
+
+274
+00:20:09,800 --> 00:20:14,680
+الزيت منتشر في المي temporary انه الزيت مع مي
+
+275
+00:20:14,680 --> 00:20:18,940
+بتعملي mix بدوبوا ببعض مجرد ما تسيب التيوب بيرجع
+
+276
+00:20:18,940 --> 00:20:23,070
+يفصل طبقتين الزيت لحال و المي لحالفال permanent
+
+277
+00:20:23,070 --> 00:20:26,990
+بيكون viscous and stable they don't separate بيكون
+
+278
+00:20:26,990 --> 00:20:30,630
+لزج قوامه لزج لأنه مادتين غير دائبين كإن إساره
+
+279
+00:20:30,630 --> 00:20:35,470
+دائبين بيكون stable مستقر ومهما تسيبيه don't
+
+280
+00:20:35,470 --> 00:20:38,590
+separate ما بيفصلوش برجعوش يفصلوا الطبقتين وإنما
+
+281
+00:20:38,590 --> 00:20:42,790
+بيضلهم دائبين بعض permanent بشكل ده أما ال
+
+282
+00:20:42,790 --> 00:20:46,540
+temporaryجاي from shaking بيحكي لإنت بتمسكي التيوب
+
+283
+00:20:46,540 --> 00:20:50,080
+و بتحطي زيت على الماء اعملي mix رشجي لتيوب بإيدك
+
+284
+00:20:50,080 --> 00:20:53,780
+بتحس إنه الزيت انتشر في الماء بمجرد ما تسيبي
+
+285
+00:20:53,780 --> 00:20:58,840
+التيوب بيرجع يفصل لطبقتين الزيت لحال و المي لحال
+
+286
+00:20:58,840 --> 00:21:03,600
+هذا بيكون temporary بشكل معقدهو رجعنا على ال slide
+
+287
+00:21:03,600 --> 00:21:07,500
+من أولها من فوق بيحكيلي هنا في عندنا أربع أربع
+
+288
+00:21:07,500 --> 00:21:13,180
+شكال على الجانب A وB وC وD في A موجودة عندى حاطين
+
+289
+00:21:13,180 --> 00:21:17,460
+خليط من مادتين سائلين مابدوبوش ببعض زى الزيت والمي
+
+290
+00:21:17,460 --> 00:21:20,820
+لو اعتبرنا انه الجزء اللى فوق هو الزيت والجزء اللى
+
+291
+00:21:20,820 --> 00:21:24,640
+تحت مي ففي أش في صورة A شايفين two immiscible
+
+292
+00:21:24,640 --> 00:21:29,040
+liquids not yet emulsified انه خليطين من خليط من
+
+293
+00:21:29,040 --> 00:21:33,550
+مادتين أصلا بدوبوش بعض immiscible لا يختلطواولا
+
+294
+00:21:33,550 --> 00:21:36,250
+حطينا لهم emulsifying agent عشان نعملهم
+
+295
+00:21:36,250 --> 00:21:40,950
+emulsification ماحطيناهاش عامل مستحيل بيساعد على
+
+296
+00:21:40,950 --> 00:21:45,030
+أنهم يدوبوا أبعاد بحكي اللي في بيه لو طلعت عليها
+
+297
+00:21:45,030 --> 00:21:48,250
+بتحس أنه الجزء اللي كان فوق انتشر في اللي تحت يعني
+
+298
+00:21:48,250 --> 00:21:52,630
+الزيت انتشر في الماء قطارات الزيت انتشرت في الماء
+
+299
+00:21:52,630 --> 00:21:56,430
+بحكي لان emulsion of phase two disappears in phase
+
+300
+00:21:56,430 --> 00:22:00,130
+one أنه انتشرت الجزء اللي فوق في الجزء اللي تحت
+
+301
+00:22:00,130 --> 00:22:05,490
+مبين كأنهم ذائبينفي C بيحكيلي unstable emulsion
+
+302
+00:22:05,490 --> 00:22:09,450
+separate كان هذا ال emulsion الاستحلاب كان مؤقت
+
+303
+00:22:09,450 --> 00:22:13,610
+كان temporary جاي من عملية shaking فمجرد ما سيبنا
+
+304
+00:22:13,610 --> 00:22:17,790
+التيوب في C ع طول فصل رجع فصل طبقتين طبقة اللي فوق
+
+305
+00:22:17,790 --> 00:22:22,590
+لحال و اللي تحت لحال الزيت فوق و المي تحت في D
+
+306
+00:22:22,590 --> 00:22:27,450
+بيحكيلي استخدمنا surfactant surfactant هو نفسه
+
+307
+00:22:27,450 --> 00:22:33,560
+emulsifying agent هو عامل مستحلباستخدم في C في D
+
+308
+00:22:33,560 --> 00:22:37,320
+استخدم surfactant عامل مستحلب Emulsifying agent
+
+309
+00:22:37,320 --> 00:22:42,280
+إيش عمل هذا العامل عمل على حشر نفسه position
+
+310
+00:22:42,280 --> 00:22:46,200
+itself on interface between face one و face two
+
+311
+00:22:46,200 --> 00:22:52,170
+حشر نفسه بين الطبقة اللي فوق و الطبقة اللي تحتيعني
+
+312
+00:22:52,170 --> 00:22:55,130
+كأنه زي الصابونة زي الـ Bile Salt اللي تاوم حكينا
+
+313
+00:22:55,130 --> 00:22:58,990
+عنها أنها بتربط بجزء من الداخل Hydrophobic ومن
+
+314
+00:22:58,990 --> 00:23:03,190
+الخارج Hydrophilic بتربط في الزيت بحيث أنه يمشي
+
+315
+00:23:03,190 --> 00:23:07,010
+بالمي كأنه دائب ففي دي أنا استخدمت Emulsifying
+
+316
+00:23:07,010 --> 00:23:10,870
+agent حشر نفسه في الزيت بين الزيت والمي وخلّى
+
+317
+00:23:10,870 --> 00:23:15,560
+الزيت كأنه دائب في الميو هذا كان بشكل permanent
+
+318
+00:23:15,560 --> 00:23:20,900
+بشكل دائم فمهما سبتيها مش هيفصل عن بعض بسبب وجود
+
+319
+00:23:20,900 --> 00:23:22,920
+ال emulsifying agent
+
+320
+00:23:25,960 --> 00:23:30,420
+كيف نعمل فحصنا لليوم؟ لنستخدم emulsifying agent ده
+
+321
+00:23:30,420 --> 00:23:35,020
+أجيب زيت و أحط عليه مي لو رجيتهم هم هيدوبوا و
+
+322
+00:23:35,020 --> 00:23:38,480
+بعدين هترجع تفصل لطبقتين تاني بس عشان يرجع تلت يوم
+
+323
+00:23:38,480 --> 00:23:42,120
+بدي أستخدم في هذا الفحص emulsifying agent أحط زيت
+
+324
+00:23:42,120 --> 00:23:46,520
+على مي و أستخدم عامل مستحلب يساعد على دوابنيتهم
+
+325
+00:23:46,520 --> 00:23:50,800
+ببعض إيش العامل المستحلب اللي بدي أستخدمه هو نصف
+
+326
+00:23:50,800 --> 00:23:56,640
+الميةNA2CO3 Sodium Bicarbonate هذا إيش وظيفته في
+
+327
+00:23:56,640 --> 00:24:01,800
+هذا الفحص وظيفته Emulsifying Agent عامل مستحل
+
+328
+00:24:01,800 --> 00:24:06,900
+وبدنا نشتغل الفحص على زيت زتون طبيعي Natural Olive
+
+329
+00:24:06,900 --> 00:24:11,740
+Oil وعلى Rancid Olive Oil على زيت زتون طبيعي وزيت
+
+330
+00:24:11,740 --> 00:24:17,180
+الزتون المتزنخnatural olive oil زيت الزيتون
+
+331
+00:24:17,180 --> 00:24:20,640
+الطبيعي و نستخدم rancid olive oil اللي هو زيت
+
+332
+00:24:20,640 --> 00:24:24,420
+الزيتون المتزنخ ايش الفرق بين الاتنين؟ بيحكي لل
+
+333
+00:24:24,420 --> 00:24:27,040
+natural olive oil اللي هو زيت الزيتون الطبيعي
+
+334
+00:24:27,040 --> 00:24:31,200
+بيكون جديد، بيكون لسه مش ساير عليه عمليات أكسدة،
+
+335
+00:24:31,200 --> 00:24:34,380
+بيكون سايرله بس partial oxidation عمليات أكسدة
+
+336
+00:24:34,380 --> 00:24:39,770
+بسيطة جزئيةلما تأكسدى انتهى أي جزاء تأكسدى هذا
+
+337
+00:24:39,770 --> 00:24:44,810
+الزيت بينتج عنه تكسير للفاتي أسد بتتكسر بتتفكك عن
+
+338
+00:24:44,810 --> 00:24:48,510
+بعضها الفاتي أسد وبتتحرر فبيصير عندى فيه free
+
+339
+00:24:48,510 --> 00:24:52,330
+fatty acid في ال natural olive oil بيكون partially
+
+340
+00:24:52,330 --> 00:24:57,690
+ال oxidation عمليات أكسدة جزئية بسيطة فكمية الفاتي
+
+341
+00:24:57,690 --> 00:25:02,370
+أسد الحرة بتكون قليلة يحتوي على few of free fatty
+
+342
+00:25:02,370 --> 00:25:06,280
+acidلكن في الـ Rancid Olive Oil في زيت الزتون
+
+343
+00:25:06,280 --> 00:25:10,060
+المتزنخ اللي هو مجلة فيه أكتر من مرة عاملينه
+
+344
+00:25:10,060 --> 00:25:14,160
+مستخدمينه أكتر من مرة بيكون صاير عليه Complete
+
+345
+00:25:14,160 --> 00:25:19,720
+Oxidation عمليات أكسدة كاملة بتأدي إلى تحرير جميع
+
+346
+00:25:19,720 --> 00:25:23,360
+الفاتي أسد فبيكون عندنا many of free fatty acid
+
+347
+00:25:23,360 --> 00:25:28,980
+الفاتي أسد بتكون الـ free الحرة بتكون بشكل أكبرإيش
+
+348
+00:25:28,980 --> 00:25:33,120
+هي Rancidity؟ Rancidity معناها التزنخ Rancidity
+
+349
+00:25:33,120 --> 00:25:38,840
+معناها التزنخ هي ظهور رائحة كريهة أو غير مرغوب
+
+350
+00:25:38,840 --> 00:25:43,800
+فيها على الزيت والزبدة وهي الرائحة غالبا بتكون
+
+351
+00:25:43,800 --> 00:25:49,680
+مصحوبة بتغير في القوام والشكل لذن ال Rancidity هو
+
+352
+00:25:49,680 --> 00:25:55,860
+التزنخ وظهور رائحة غير مرغوب فيهامصحوبة بتغيير في
+
+353
+00:25:55,860 --> 00:25:59,740
+القوام والشكل على الزيت والزبدة
+
+354
+00:26:02,130 --> 00:26:05,870
+مش خطواتنا كيف نشتغل الفحص تبع ال emulsification
+
+355
+00:26:05,870 --> 00:26:09,650
+إذا انفهمنا أن ال objective من الفحص أن أنا بدي
+
+356
+00:26:09,650 --> 00:26:14,150
+أعمل emulsified oil and water in each other by
+
+357
+00:26:14,150 --> 00:26:18,410
+emulsifying agent بدي أساعد ال oil والwater، المي
+
+358
+00:26:18,410 --> 00:26:22,530
+والزيت ان هم يصيروا emulsified بعض بمساعدة من؟
+
+359
+00:26:22,530 --> 00:26:27,150
+emulsifying agentاللي هو عبارة عن الـ Sodium
+
+360
+00:26:27,150 --> 00:26:30,870
+Bicarbonate فلو كتبتوا .. كتبنا لبرنزميل عن النظام
+
+361
+00:26:30,870 --> 00:26:35,730
+معادلة ده هذا الفحص أحكي fat أو oil اللي هو الزيت
+
+362
+00:26:35,730 --> 00:26:39,690
+مع water بدي أضيف عليه ميه في وجود emulsifying
+
+363
+00:26:39,690 --> 00:26:42,950
+agent اللي هو Sodium Bicarbonate إيش بدي يصير
+
+364
+00:26:42,950 --> 00:26:46,490
+عندي؟ بدي يصير عندي emulsification بدي يصير عندي
+
+365
+00:26:46,490 --> 00:26:50,740
+emulsification الناتجخطوات العمل بدي أجيب تالتة
+
+366
+00:26:50,740 --> 00:26:54,320
+تيوب بقى تيوب الأول بدي أشتغل عليه بدي أحط فيه
+
+367
+00:26:54,320 --> 00:26:58,020
+drop من ال oil زيت زتون مع 2 ml distilled water و
+
+368
+00:26:58,020 --> 00:27:02,740
+أعمل mix بس حطيت زيت مع مي و عملتي mix إيش بدي
+
+369
+00:27:02,740 --> 00:27:06,580
+يصير؟ بتلاقي الزيت قطرات الزيت بتنتشر في المي و
+
+370
+00:27:06,580 --> 00:27:10,620
+مجرد ما تسيبي التيوب بيرجع يفصل لطبقتين يعني هو
+
+371
+00:27:10,620 --> 00:27:16,110
+insoluble لإن زيت مع مي بدوبوشالتيوب التاني بدي
+
+372
+00:27:16,110 --> 00:27:19,990
+تحطي عليها خمسة مل من الـ Distilled water على
+
+373
+00:27:19,990 --> 00:27:23,850
+اتنين لتلاتة drop من ال Sodium Bicarbonate على
+
+374
+00:27:23,850 --> 00:27:27,910
+drop من ال Oil تحط خمسة مل Distilled water اتنين
+
+375
+00:27:27,910 --> 00:27:30,750
+لتلاتة drop من ال Sodium Bicarbonate اللي هو ال
+
+376
+00:27:30,750 --> 00:27:34,710
+emulsifying agent على drop من الزيت لجيت حطينا زيت
+
+377
+00:27:34,710 --> 00:27:38,070
+مع مي و حطينا عليهم emulsifying agent المفروض انه
+
+378
+00:27:38,070 --> 00:27:43,170
+يساعد على عمل emulsification و يبينوا كأنهم دائبين
+
+379
+00:27:43,170 --> 00:27:48,270
+في بعضلكن بيحكيلي في هذه اليوتيوب لأن انت حاطيتي
+
+380
+00:27:48,270 --> 00:27:52,270
+oil natural olive oil زيت الزيتون الطبيعي الذي
+
+381
+00:27:52,270 --> 00:27:57,090
+يحتوي على كمية قليلة من ال free fatty acid بيقدرش
+
+382
+00:27:57,090 --> 00:28:01,050
+ييجي ال sodium bicarbonate يحشر بينهم ويرتبط معها
+
+383
+00:28:01,050 --> 00:28:04,350
+مافيش free fatty acid حرة انه يقدر يرتبط معها
+
+384
+00:28:04,350 --> 00:28:08,250
+ويعمل emulsificationفبيكون هنا بيعمل بشكل بس
+
+385
+00:28:08,250 --> 00:28:12,170
+temporary بتحس إنه الزيت مع المي إنهم دابوا ببعض
+
+386
+00:28:12,170 --> 00:28:16,430
+لكن سرعان ما يفصلوا عن بعض يفصل لطبقتين فبيكون
+
+387
+00:28:16,430 --> 00:28:19,410
+عبارة عن temporary emulsification صار بشكل
+
+388
+00:28:19,410 --> 00:28:23,590
+temporary ليش؟ لأن ال natural olive oil يحتوي على
+
+389
+00:28:23,590 --> 00:28:27,610
+few free fatty acids كمية الحماض الدهنية ال free
+
+390
+00:28:27,610 --> 00:28:32,580
+بتكون قليلةالتيوب التالت بدي أضع عليها 5 مل من
+
+391
+00:28:32,580 --> 00:28:35,960
+الـDistill water على 2 إلى 3 مل من الـ Sodium
+
+392
+00:28:35,960 --> 00:28:39,520
+bicarbonate اللي هو الـ Emulsifying agent بدي أضع
+
+393
+00:28:39,520 --> 00:28:44,040
+drop من الـ Rancid oil من الزيت الزتون المتزنخ
+
+394
+00:28:44,040 --> 00:28:48,580
+متزنخ يعني يحتوي على كمية كبيرة من الـ Free fatty
+
+395
+00:28:48,580 --> 00:28:53,700
+acid حطيتي منها هذا بيقدر ييجي الـ Emulsifying
+
+396
+00:28:53,700 --> 00:28:57,060
+agent اللي هي Sodium carbonate أنه يرتبط مع الـ
+
+397
+00:28:57,060 --> 00:29:01,040
+Free fatty acid الحرة هذهو يعمل emulsification
+
+398
+00:29:01,040 --> 00:29:06,680
+بشكل permanent بيكون بشكل permanent بشكل دائم،
+
+399
+00:29:06,680 --> 00:29:14,160
+دائمين بعض بشكل دائم الفحص التالت هو oxidation of
+
+400
+00:29:14,160 --> 00:29:18,500
+unsaturated fatty acid بتدعم الأكسدة لل
+
+401
+00:29:18,500 --> 00:29:22,780
+unsaturated fatty acidبعدين في اكسدة في تفاعلات
+
+402
+00:29:22,780 --> 00:29:26,220
+اكسدة واختزال اذا انا محتاجة ل oxidizing agent
+
+403
+00:29:26,220 --> 00:29:31,180
+لعامل مؤكسد عشان ي oxidize ال unsaturated fatty
+
+404
+00:29:31,180 --> 00:29:35,080
+acid ايش يعني unsaturated fatty acid حكينا
+
+405
+00:29:35,080 --> 00:29:39,000
+unsaturated معناه انه بيحتوي على double bond على
+
+406
+00:29:39,000 --> 00:29:43,480
+one or more double bond عنده double bond واحدة او
+
+407
+00:29:43,480 --> 00:29:47,860
+اكترفي عندنا نوعين من ال Acid هدول اللي بنستخدمهم
+
+408
+00:29:47,860 --> 00:29:51,040
+في عندنا Olic Acid هو عبارة عن Unsaturated Fatty
+
+409
+00:29:51,040 --> 00:29:55,620
+Acid وفي Steric Acid هو Saturated Fatty Acid
+
+410
+00:29:55,620 --> 00:29:59,260
+بنستخدم هدول اتنين في هذا الفحص اليوم مين اللي
+
+411
+00:29:59,260 --> 00:30:02,940
+هيعطيني positive بس ال Unsaturated Fatty Acid اللي
+
+412
+00:30:02,940 --> 00:30:06,800
+هو ال Olic Acid ال Olic Acid هيتغير لونه ويعطيني
+
+413
+00:30:06,800 --> 00:30:10,720
+positive لأنه بيصيرله أكس دهأما الـ stearic acid
+
+414
+00:30:10,720 --> 00:30:14,940
+بيصيرش عليه أكثر ده لأنه عبارة عن saturated fatty
+
+415
+00:30:14,940 --> 00:30:20,230
+acidكيف بيدعم ال Oxidation؟ بدي Oxidizing agent
+
+416
+00:30:20,230 --> 00:30:24,230
+اللي هو عامل مؤكسد العامل المؤكسد اللي بنستخدمه في
+
+417
+00:30:24,230 --> 00:30:27,590
+فحصنا لليوم في هذا الفحص هو ال potassium
+
+418
+00:30:27,590 --> 00:30:34,150
+permanganate KMnO4 potassium permanganate لو حكيتش
+
+419
+00:30:34,150 --> 00:30:38,070
+ال objective لهذا الفحص to oxidize oleic acid by
+
+420
+00:30:38,070 --> 00:30:42,450
+potassium permanganate بيدعم الأكسدة للacid
+
+421
+00:30:42,450 --> 00:30:46,830
+باستخدام البوتاسيوم بيرمانجانات ولو بدك تكتب ال
+
+422
+00:30:46,830 --> 00:30:50,470
+principle على شكل معادلة بحكي ان ال acid اللي انا
+
+423
+00:30:50,470 --> 00:30:54,690
+بده اعمله اكسدا زي ال potassium بيرمانجانات بيحكي
+
+424
+00:30:54,690 --> 00:30:58,930
+اللي بيعطيني لون brownالأولك أسد مع بوتاسيوم
+
+425
+00:30:58,930 --> 00:31:02,990
+بيرمانجانيت العامل المؤكسل يعمل على أكسدة الأولك
+
+426
+00:31:02,990 --> 00:31:06,870
+أسد ويتحول اللون إلى لون براون البوتاسيوم
+
+427
+00:31:06,870 --> 00:31:10,610
+بيرمانجانيت أصلا لونه violet لونه move لونه purple
+
+428
+00:31:10,610 --> 00:31:15,150
+على violet move لما تحطيه على الأولك أسد بيعمل
+
+429
+00:31:15,150 --> 00:31:21,000
+عملية أكسدة للأولك أسد بيحوله إلى لون براونكيف
+
+430
+00:31:21,000 --> 00:31:24,420
+خطوات إجراء التجربة؟ بدي أشوف بالتيوب بدي أشتغل
+
+431
+00:31:24,420 --> 00:31:27,180
+فيها أوليك أسد والتيوب بدي أشتغل فيها استيريك أسد
+
+432
+00:31:27,180 --> 00:31:31,160
+و أشوف من يغير أول تيوب بدي أشتغل فيها أوليك أسد
+
+433
+00:31:31,160 --> 00:31:34,520
+بدي أشتغل فيه one drop of oleic acid على تلاتة مل
+
+434
+00:31:34,520 --> 00:31:38,120
+من ال sodium bicarbonate على اتنين مل من
+
+435
+00:31:38,120 --> 00:31:44,670
+البوتاسيوم بيرمانجنانتمام ده حاط واحد drop من
+
+436
+00:31:44,670 --> 00:31:48,090
+الolic acid على 3 مل من ال sodium bicarbonate على
+
+437
+00:31:48,090 --> 00:31:51,690
+2 مل من ال potassium permanganate الolic acid هو
+
+438
+00:31:51,690 --> 00:31:55,470
+عبارة عن unsaturated fatty acid ييجي ال potassium
+
+439
+00:31:55,470 --> 00:31:59,550
+permanganate بيعمله أكسدة يفترض يتحول اللون إلى
+
+440
+00:31:59,550 --> 00:32:04,390
+brown إذا positive إذا هو olic acidالتيوب التانية
+
+441
+00:32:04,390 --> 00:32:08,470
+بده حط drop من ال stearic acid على 3 مل من ال
+
+442
+00:32:08,470 --> 00:32:12,670
+sodium bicarbonate على 2 مل من ال potassium
+
+443
+00:32:12,670 --> 00:32:16,530
+permanganate بيحكي اللي لما تحطي ال stearic acid
+
+444
+00:32:16,530 --> 00:32:20,150
+هو عبارة عن unsaturated fatty acid فلما تحط عليه
+
+445
+00:32:20,150 --> 00:32:24,550
+ال potassium permanganate بيتغيرش اللون وانما بيظل
+
+446
+00:32:24,550 --> 00:32:31,930
+لونه violet مش هيتغير مش هيتحول للون ل brownأخر
+
+447
+00:32:31,930 --> 00:32:35,790
+فحص هو السلكا ويسكي reaction هذا السلكا ويسكي
+
+448
+00:32:35,790 --> 00:32:41,530
+reaction هو فحص للكشف عن الزيت بستخدم زيت بدي أشوف
+
+449
+00:32:41,530 --> 00:32:45,590
+هل هذا الزيت مخشوش مضعف عليه الكلسترول ولا مش مضعف
+
+450
+00:32:45,590 --> 00:32:51,280
+هذا خاص للكشف هذا الفحص السلكا ويسكي reactionللكشف
+
+451
+00:32:51,280 --> 00:32:55,020
+عن الكوليسترول، هل الكوليسترول موجود ولا لا؟ في
+
+452
+00:32:55,020 --> 00:32:58,320
+ناس بتغش الزيت، بتضيف عليه كوليسترول أنا بدي أشوف
+
+453
+00:32:58,320 --> 00:33:01,380
+هل هذا الزيت مضاف عليه كوليسترول ولا هو زيت زيتون
+
+454
+00:33:01,380 --> 00:33:07,040
+طبيعي؟ كيف مبدأ عمله؟ هذا الفحص مبدأ عمله بيتم على
+
+455
+00:33:07,040 --> 00:33:12,880
+استخدام concentrated sulfuric acid H2SO4 نستخدم
+
+456
+00:33:12,880 --> 00:33:18,420
+concentrated sulfuric acid اللي هو H2SO4هذا حساس
+
+457
+00:33:18,420 --> 00:33:22,900
+للرطوبة «highly hygroscopic» هو حساس للرطوبة لا
+
+458
+00:33:22,900 --> 00:33:25,720
+يمكن للطيوبات اللي أشتغل فيها يكون فيها ماء لازم
+
+459
+00:33:25,720 --> 00:33:31,080
+تكون جافة لأن أصلا مبدأ عمله يعمل على «removal of
+
+460
+00:33:31,080 --> 00:33:33,880
+two molecules of water from two molecules of
+
+461
+00:33:33,880 --> 00:33:38,120
+cholesterol» إن إذا الكوليسترول موجود في الزيتإذا
+
+462
+00:33:38,120 --> 00:33:43,260
+كان في عندي في الزيت موجود كوليسترال بيجي الـH2SO4
+
+463
+00:33:43,260 --> 00:33:46,720
+الـ concentrated sulfuric acid هاد بيقوم بنزع
+
+464
+00:33:46,720 --> 00:33:51,660
+جزيئين ماء من جزيئين كوليسترال إذا في كوليسترال
+
+465
+00:33:51,660 --> 00:33:58,020
+بيجي الـH2SO4 بينزع جزيئين ماء من هذا الكوليسترال
+
+466
+00:33:58,020 --> 00:34:03,310
+من position من موقع تلاتة تلاتةنزع جزيئين ماء من
+
+467
+00:34:03,310 --> 00:34:07,650
+اتنين كلسترول من موقع تلاتة و تلاتة بينتج النتج
+
+468
+00:34:07,650 --> 00:34:10,950
+بيتحول لكلسترول لما تنزع منه جزئين ماء بيتحول
+
+469
+00:34:10,950 --> 00:34:16,050
+لحاجة اسمها bi-cholestadineالـ GTBG كمان مر
+
+470
+00:34:16,050 --> 00:34:20,750
+الصلفوريك أسد هذا بيشتغل على البيكلستادين بيعمله
+
+471
+00:34:20,750 --> 00:34:26,930
+صلفونات بيكلستادين at position 7 و7 بيعمل صلفونات
+
+472
+00:34:26,930 --> 00:34:31,150
+للبيكلستادين بيضيف عليه صلفية ال group بتحول إلى
+
+473
+00:34:31,150 --> 00:34:36,090
+حاجة اسمة bi-sulfonic acid bi-cholestadine لونه
+
+474
+00:34:36,090 --> 00:34:41,200
+red colorإذا بفحص السلكا whisk reaction و فحص
+
+475
+00:34:41,200 --> 00:34:44,420
+للكشف عن الكوليسترول هل موجود في زيتها المضاف عليه
+
+476
+00:34:44,420 --> 00:34:50,260
+كوليسترول ولا لأ؟كيف باستخدام Sulphuric Acid؟ إيش
+
+477
+00:34:50,260 --> 00:34:53,900
+وظيفة Sulphuric Acid في فحص السلكويسكي reaction؟
+
+478
+00:34:53,900 --> 00:34:58,280
+الوظيفتين أول وظيفة remove two molecules of water
+
+479
+00:34:58,280 --> 00:35:02,440
+from two molecules of كوليسترول نزع جزيئين ماء من
+
+480
+00:35:02,440 --> 00:35:06,520
+ال .. من اتنين كوليسترول من موقع تلاتة ويتحول
+
+481
+00:35:06,520 --> 00:35:10,590
+الكوليسترول لحاجة اسمها bi-cholestadineبعدين
+
+482
+00:35:10,590 --> 00:35:13,990
+الوظيفة التانية يعمل صلفونية بيكلستادين at
+
+483
+00:35:13,990 --> 00:35:18,350
+position 7 و7 اللي هي إضافة صلفية ال group على
+
+484
+00:35:18,350 --> 00:35:22,450
+position 7 و7 في البيكلستادين بتكون حاجة اسمها
+
+485
+00:35:22,450 --> 00:35:27,810
+bisulfonic acid bicolestidine لونها red colorإذا
+
+486
+00:35:27,810 --> 00:35:30,650
+ال objective من هذا الفحص to detect the presence
+
+487
+00:35:30,650 --> 00:35:35,890
+of cholesterol كيف مابدأ أعمله؟ إنه بيكون عندى
+
+488
+00:35:35,890 --> 00:35:39,590
+اتنين كلسترول إذا عندى مركبين كلسترول بنحط عليهم
+
+489
+00:35:39,590 --> 00:35:44,590
+concentrated sulfuric acid H2SO4 بيتحول النتج نائي
+
+490
+00:35:44,590 --> 00:35:49,210
+ليش؟ by sulfonate by كوليستادين لونه red
+
+491
+00:35:54,630 --> 00:35:58,110
+هنا عملنا structure لبيكولستادين كيف شكله و
+
+492
+00:35:58,110 --> 00:36:02,390
+بيسالفونك أسد بيكولستادين كيف شكله عشان أطلب مننا
+
+493
+00:36:02,390 --> 00:36:07,650
+ال structureإيش ال .. كيف خطوات التجربة أو إيش
+
+494
+00:36:07,650 --> 00:36:10,670
+نستخدم ال agent نستخدم زيت زتون مضاعف عليه
+
+495
+00:36:10,670 --> 00:36:15,030
+الكوليسترول نستخدم زيت زتون طبيعي و زيت زتون مضاعف
+
+496
+00:36:15,030 --> 00:36:18,430
+عليه الكوليسترول عشان نشوف الفان و نستخدم ال
+
+497
+00:36:18,430 --> 00:36:22,330
+chloroform عشان ندوّب بس الزيت عشان أقدر أدوّب
+
+498
+00:36:22,330 --> 00:36:28,350
+الزيت و نتجنب استنشاق كمية من هذا ال chloroform
+
+499
+00:36:28,350 --> 00:36:31,550
+لأن هذا بيأثر على ال central nervous system على
+
+500
+00:36:31,550 --> 00:36:37,080
+الجهاز العصبي بسبب نازيا و headacheو business صداع
+
+501
+00:36:37,080 --> 00:36:43,900
+و غثيان و كوم داخل و بدنا نستخدم ال sulfuric acid
+
+502
+00:36:43,900 --> 00:36:48,640
+اللي له وظيفتين في هذا الفحص حكينا عنهم كيف طريقة
+
+503
+00:36:48,640 --> 00:36:52,920
+إجراء التجربة ده جيب كيبين واحد استغل فيها الزيت
+
+504
+00:36:52,920 --> 00:36:56,620
+الزيتون من غير كلسترول و واحدة بدأ استخدم فيها زيت
+
+505
+00:36:56,620 --> 00:37:00,920
+زيتون مضاف عليه كلسترول عشان أشوف الفانأول تيوب
+
+506
+00:37:00,920 --> 00:37:07,920
+سأضع عليها 1ml كلوروفارم 1ml كلوروفارم على 2 drop
+
+507
+00:37:07,920 --> 00:37:13,720
+من الزيت 1ml كلوروفارم على 2 drop من الزيت ثم أضع
+
+508
+00:37:13,720 --> 00:37:18,300
+2ml من H2اسوفارم هجد زيت زيتون إذا كان طبيعي مش
+
+509
+00:37:18,300 --> 00:37:22,340
+هيتغير إشي وهيكون negative في الشلون تيوب التاني
+
+510
+00:37:22,340 --> 00:37:27,400
+سأضع عليها 1ml كلوروفارمو اتنين drop من
+
+511
+00:37:27,400 --> 00:37:30,820
+الكوليسترول زيت زيتون مضاف عليه كوليسترول و احط
+
+512
+00:37:30,820 --> 00:37:35,460
+اتنين مل من H2SO فان بلاحظ ان اللون عندى بيتحول
+
+513
+00:37:35,460 --> 00:37:39,620
+الى red اذا اتكونت ال red اللون الأحمر اذا هذا
+
+514
+00:37:39,620 --> 00:37:47,590
+الزيت مضاف عليه كوليسترولإذا ما اتحولش إذا هو زيت
+
+515
+00:37:47,590 --> 00:37:51,410
+زتون طبيعي بدون كوليسترول بيحكيلي في أول إشي انه
+
+516
+00:37:51,410 --> 00:37:56,050
+انه تكون ال dry test tube تكون لتيوبات جافة ضروري
+
+517
+00:37:56,050 --> 00:37:59,190
+لتيوبات تكون جافة لأنه هذا حكينا أنه highly
+
+518
+00:37:59,190 --> 00:38:03,530
+hygroscopic حساس للرطوبة لأن أصلا المبدأ عامله أنه
+
+519
+00:38:03,530 --> 00:38:07,350
+بده جزيئين ما ينزع جزيئين ماإذا التيوب فيها مي
+
+520
+00:38:07,350 --> 00:38:11,250
+هياخد المي الموجودة في التيوب مش هيشتغل على
+
+521
+00:38:11,250 --> 00:38:15,510
+الكوليسترول إذا موجود انما هيشتغل على المي اللي في
+
+522
+00:38:15,510 --> 00:38:21,410
+التيوب بدل الكوليسترول هيك بنكون خلصنا هذا المعمل،
+
+523
+00:38:21,410 --> 00:38:25,010
+المعمل الليبد ان شاء الله نشتغل عملي فيه المعمل،
+
+524
+00:38:25,010 --> 00:38:30,680
+مطلوب منكمتكتبولي ال principle لكل فحص على شكل
+
+525
+00:38:30,680 --> 00:38:35,640
+معادلات و اللي مش معادلة تكتبولي المبدأ برضه
+
+526
+00:38:35,640 --> 00:38:37,080
+يعطيكم العافية
+
diff --git a/PL9fwy3NUQKwZ1Q7zOg1ObgufPMjRI3WY1/VOnoEDYqP7I_raw.json b/PL9fwy3NUQKwZ1Q7zOg1ObgufPMjRI3WY1/VOnoEDYqP7I_raw.json
new file mode 100644
index 0000000000000000000000000000000000000000..c3c74b9b3e8154eeace70790d537fc3718bdb1f1
--- /dev/null
+++ b/PL9fwy3NUQKwZ1Q7zOg1ObgufPMjRI3WY1/VOnoEDYqP7I_raw.json
@@ -0,0 +1 @@
+{"segments": [{"id": 1, "seek": 2871, "start": 0.77, "end": 28.71, "text": "اليوم ان شاء الله بدنا نحكي في موضوع جديد اللي هو موضوع الدهون بدنا نحكي عن ال libid عن الدهون نتعرف عليها وعلى فحوصات للكشف عن الدهون في البداية بدنا نحكي تعريف ال libid ال libid هي group of naturally occurring substance consisting of higher fatty acid وهي naturally occurring substance أو compound", "tokens": [6027, 1829, 20498, 16472, 13412, 16606, 21984, 47525, 8315, 8717, 5016, 4117, 1829, 8978, 3714, 2407, 11242, 45367, 10874, 16254, 3215, 13672, 1829, 31439, 3714, 2407, 11242, 45367, 32748, 3224, 11536, 47525, 8315, 8717, 5016, 4117, 1829, 18871, 2423, 22854, 327, 18871, 32748, 3224, 11536, 8717, 2655, 3615, 28480, 25894, 11296, 4032, 3615, 23942, 6156, 5016, 2407, 9381, 9307, 24976, 4117, 8592, 5172, 18871, 32748, 3224, 11536, 8978, 29739, 28259, 10632, 47525, 8315, 8717, 5016, 4117, 1829, 37279, 16572, 5172, 2423, 22854, 327, 2423, 22854, 327, 39896, 1594, 295, 8195, 18386, 12961, 33921, 295, 2946, 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184.71, "end": 184.95, "word": " هذه", "probability": 0.8291015625}, {"start": 184.95, "end": 185.67, "word": " المكونات", "probability": 0.94873046875}, {"start": 185.67, "end": 186.07, "word": " عبارة", "probability": 0.998046875}, {"start": 186.07, "end": 186.21, "word": " عن", "probability": 0.99560546875}, {"start": 186.21, "end": 186.53, "word": " lipid", "probability": 0.842041015625}, {"start": 186.53, "end": 187.39, "word": " فأغلب", "probability": 0.9879150390625}, {"start": 187.39, "end": 188.11, "word": " مكونات", "probability": 0.93486328125}, {"start": 188.11, "end": 188.27, "word": " ال", "probability": 0.943359375}, {"start": 188.27, "end": 188.45, "word": " cell", "probability": 0.951171875}, {"start": 188.45, "end": 188.83, "word": " membrane", "probability": 0.9521484375}, {"start": 188.83, "end": 189.21, "word": " هي", "probability": 0.97216796875}, {"start": 189.21, "end": 189.93, "word": " عبارة", "probability": 0.99853515625}, {"start": 189.93, "end": 190.33, "word": " عن", "probability": 0.99609375}, {"start": 190.33, "end": 191.23, "word": " lipid", "probability": 0.949951171875}, {"start": 191.23, "end": 191.71, "word": " فهو", "probability": 0.9241536458333334}, {"start": 191.71, "end": 192.37, "word": " التركيبة", "probability": 0.9654541015625}, {"start": 192.37, "end": 193.29, "word": " الأساسية", "probability": 0.9879150390625}, {"start": 193.29, "end": 193.65, "word": " ل", "probability": 0.68896484375}, {"start": 193.65, "end": 193.97, "word": " ال", "probability": 0.390625}, {"start": 193.97, "end": 194.29, "word": " cell", "probability": 0.951171875}, {"start": 194.29, "end": 194.83, "word": " membrane", "probability": 0.958984375}], "temperature": 1.0}, {"id": 9, "seek": 22370, "start": 197.98, "end": 223.7, "text": "أنواع الدهون type of lipid إيش أنواع الدهون الدهون قُسمت بشكل عام إلى lipid with fatty acid و lipid without fatty acid يعني الدهون بشكل عام قُسمت إلى قسمين دهون تحتوي على fatty acid ودهون لا تحتوي على fatty acid lipid with fatty acid و lipid without fatty acid", "tokens": [33456, 14407, 3615, 32748, 3224, 11536, 2010, 295, 8280, 327, 11933, 1829, 8592, 14739, 14407, 3615, 32748, 3224, 11536, 32748, 3224, 11536, 12174, 10859, 38251, 2655, 4724, 8592, 28820, 6225, 10943, 30731, 8280, 327, 365, 24898, 8258, 4032, 8280, 327, 1553, 24898, 8258, 37495, 22653, 32748, 3224, 11536, 4724, 8592, 28820, 6225, 10943, 12174, 10859, 38251, 2655, 30731, 12174, 38251, 9957, 11778, 3224, 11536, 6055, 33753, 45865, 15844, 24898, 8258, 4032, 3215, 3224, 11536, 20193, 6055, 33753, 45865, 15844, 24898, 8258, 8280, 327, 365, 24898, 8258, 4032, 8280, 327, 1553, 24898, 8258], "avg_logprob": -0.09232190732033022, "compression_ratio": 2.310126582278481, "no_speech_prob": 0.0, "words": [{"start": 197.98, "end": 198.54, "word": "أنواع", "probability": 0.90673828125}, {"start": 198.54, "end": 199.02, "word": " الدهون", "probability": 0.9705403645833334}, {"start": 199.02, "end": 199.66, "word": " type", "probability": 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{"start": 206.28, "end": 206.82, "word": " fatty", "probability": 0.96240234375}, {"start": 206.82, "end": 207.4, "word": " acid", "probability": 0.93603515625}, {"start": 207.4, "end": 207.94, "word": " و", "probability": 0.9638671875}, {"start": 207.94, "end": 208.36, "word": " lipid", "probability": 0.925048828125}, {"start": 208.36, "end": 209.1, "word": " without", "probability": 0.93408203125}, {"start": 209.1, "end": 209.7, "word": " fatty", "probability": 0.9580078125}, {"start": 209.7, "end": 210.2, "word": " acid", "probability": 0.95263671875}, {"start": 210.2, "end": 210.96, "word": " يعني", "probability": 0.58648681640625}, {"start": 210.96, "end": 211.66, "word": " الدهون", "probability": 0.9920247395833334}, {"start": 211.66, "end": 212.22, "word": " بشكل", "probability": 0.990234375}, {"start": 212.22, "end": 212.78, "word": " عام", "probability": 0.9990234375}, {"start": 212.78, "end": 213.86, "word": " قُسمت", "probability": 0.9896240234375}, {"start": 213.86, "end": 214.06, "word": " إلى", "probability": 0.9716796875}, {"start": 214.06, "end": 214.72, "word": " قسمين", "probability": 0.9493815104166666}, {"start": 214.72, "end": 215.72, "word": " دهون", "probability": 0.9348958333333334}, {"start": 215.72, "end": 216.4, "word": " تحتوي", "probability": 0.9669596354166666}, {"start": 216.4, "end": 216.66, "word": " على", "probability": 0.9365234375}, {"start": 216.66, "end": 217.06, "word": " fatty", "probability": 0.9501953125}, {"start": 217.06, "end": 217.56, "word": " acid", "probability": 0.96728515625}, {"start": 217.56, "end": 218.5, "word": " ودهون", "probability": 0.9014892578125}, {"start": 218.5, "end": 218.72, "word": " لا", "probability": 0.92236328125}, {"start": 218.72, "end": 219.36, "word": " تحتوي", "probability": 0.9827473958333334}, {"start": 219.36, "end": 219.62, "word": " على", "probability": 0.9521484375}, {"start": 219.62, "end": 220.08, "word": " fatty", "probability": 0.9541015625}, {"start": 220.08, "end": 220.44, "word": " acid", "probability": 0.96337890625}, {"start": 220.44, "end": 221.12, "word": " lipid", "probability": 0.92724609375}, {"start": 221.12, "end": 221.32, "word": " with", "probability": 0.923828125}, {"start": 221.32, "end": 221.64, "word": " fatty", "probability": 0.96728515625}, {"start": 221.64, "end": 222.04, "word": " acid", "probability": 0.95654296875}, {"start": 222.04, "end": 222.2, "word": " و", "probability": 0.97509765625}, {"start": 222.2, "end": 222.58, "word": " lipid", "probability": 0.905517578125}, {"start": 222.58, "end": 222.94, "word": " without", "probability": 0.931640625}, {"start": 222.94, "end": 223.34, "word": " fatty", "probability": 0.9619140625}, {"start": 223.34, "end": 223.7, "word": " acid", "probability": 0.9609375}], "temperature": 1.0}, {"id": 10, "seek": 24208, "start": 224.74, "end": 242.08, "text": "اللي with fatty acid تحتوي على fatty acid من الامتل عليها ال waxes، fat and oil، الذبدة ويئ الزيت، phospholipid، sphingolipid، lipid without fatty acid زي الستيرون، هاي التقسيمة بشكل عام وهالجيتها نبدأ نفرع منها", "tokens": [6027, 20292, 365, 24898, 8258, 6055, 33753, 45865, 15844, 24898, 8258, 9154, 2423, 10943, 2655, 1211, 25894, 11296, 2423, 17352, 279, 12399, 4046, 293, 3184, 12399, 32545, 3555, 41891, 4032, 1829, 19986, 6024, 110, 36081, 12399, 19775, 401, 647, 327, 12399, 637, 571, 401, 647, 327, 12399, 8280, 327, 1553, 24898, 8258, 30767, 1829, 2423, 14851, 13546, 11536, 12399, 8032, 47302, 16712, 4587, 3794, 32640, 3660, 4724, 8592, 28820, 6225, 10943, 37037, 6027, 7435, 36081, 11296, 8717, 44510, 10721, 8717, 5172, 2288, 3615, 9154, 11296], "avg_logprob": -0.23092296147762342, "compression_ratio": 1.5824742268041236, "no_speech_prob": 0.0, "words": [{"start": 224.74, "end": 225.06, "word": "اللي", "probability": 0.781005859375}, {"start": 225.06, "end": 225.32, "word": " with", "probability": 0.403564453125}, {"start": 225.32, "end": 225.64, "word": " fatty", "probability": 0.958984375}, {"start": 225.64, "end": 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0.829833984375}, {"start": 230.86, "end": 231.28, "word": " الذبدة", "probability": 0.7103678385416666}, {"start": 231.28, "end": 231.6, "word": " ويئ", "probability": 0.5521647135416666}, {"start": 231.6, "end": 232.52, "word": " الزيت،", "probability": 0.75823974609375}, {"start": 232.52, "end": 233.76, "word": " phospholipid،", "probability": 0.8962890625}, {"start": 233.76, "end": 235.08, "word": " sphingolipid،", "probability": 0.8701171875}, {"start": 235.08, "end": 235.64, "word": " lipid", "probability": 0.6893310546875}, {"start": 235.64, "end": 236.04, "word": " without", "probability": 0.92724609375}, {"start": 236.04, "end": 236.58, "word": " fatty", "probability": 0.970703125}, {"start": 236.58, "end": 237.0, "word": " acid", "probability": 0.916015625}, {"start": 237.0, "end": 237.38, "word": " زي", "probability": 0.96875}, {"start": 237.38, "end": 238.74, "word": " الستيرون،", "probability": 0.659423828125}, {"start": 238.74, "end": 238.96, "word": " هاي", "probability": 0.654052734375}, {"start": 238.96, "end": 239.54, "word": " التقسيمة", "probability": 0.91796875}, {"start": 239.54, "end": 239.9, "word": " بشكل", "probability": 0.9847005208333334}, {"start": 239.9, "end": 240.28, "word": " عام", "probability": 0.998291015625}, {"start": 240.28, "end": 240.76, "word": " وهالجيتها", "probability": 0.6201171875}, {"start": 240.76, "end": 241.24, "word": " نبدأ", "probability": 0.9874674479166666}, {"start": 241.24, "end": 241.76, "word": " نفرع", "probability": 0.9373779296875}, {"start": 241.76, "end": 242.08, "word": " منها", "probability": 0.99462890625}], "temperature": 1.0}, {"id": 11, "seek": 27273, "start": 243.47, "end": 272.73, "text": "لقيت احنا حاكي هنا a classification هنا of lipid نبدأ نفعلها اللبد بشكل عام تنقسم إلى lipid with fatty acid تحتوي على fatty acid و lipid without fatty acid ده تحتوي على fatty acid ناخد الجزء الأول اللي هي اللبد with fatty acid اللي بتحتوي على fatty acid اللبد اللتي تحتوي على fatty acid تنقسم إلى قسمين في منها simple lipid و في منها conjugate في simple", "tokens": [1211, 4587, 36081, 1975, 5016, 8315, 11331, 995, 4117, 1829, 34105, 257, 21538, 34105, 295, 8280, 327, 8717, 44510, 10721, 8717, 5172, 30241, 11296, 13672, 3555, 3215, 4724, 8592, 28820, 6225, 10943, 6055, 1863, 4587, 38251, 30731, 8280, 327, 365, 24898, 8258, 6055, 33753, 45865, 15844, 24898, 8258, 4032, 8280, 327, 1553, 24898, 8258, 11778, 3224, 6055, 33753, 45865, 15844, 24898, 8258, 8717, 47283, 3215, 25724, 11622, 38207, 16247, 12610, 13672, 1829, 39896, 13672, 3555, 3215, 365, 24898, 8258, 13672, 1829, 39894, 33753, 45865, 15844, 24898, 8258, 13672, 3555, 3215, 13672, 31371, 6055, 33753, 45865, 15844, 24898, 8258, 6055, 1863, 4587, 38251, 30731, 12174, 38251, 9957, 8978, 9154, 11296, 2199, 8280, 327, 4032, 8978, 9154, 11296, 45064, 8978, 2199], "avg_logprob": -0.15234375384946663, "compression_ratio": 2.1913043478260867, "no_speech_prob": 0.0, "words": [{"start": 243.47, "end": 243.79, "word": "لقيت", "probability": 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"end": 329.94, "text": "إذا فاتي أسد متصلة بكحول بسميها سم باللبن طب الكحول هاد إما بيكون ممكن يكون نوعه إيش هيكون نوعه ممكن يكون glycerol أو حاجة تانية كحول نوع تاني غير ال glycerol كيف بيرتبط الفاتي أسد مع الكحول ب Easter bond by Easter bond اللي هي الرابطة الدهنية هذا اسم الرابطة الدهنية بيرتبط الفاتي أسد مع الكحول برابطة دهنية", "tokens": [28814, 15730, 6156, 9307, 1829, 5551, 3794, 3215, 44650, 36520, 3660, 4724, 4117, 5016, 12610, 4724, 38251, 1829, 11296, 8608, 2304, 20666, 46152, 1863, 23032, 3555, 33251, 5016, 12610, 8032, 18513, 11933, 15042, 4724, 1829, 30544, 3714, 43020, 7251, 30544, 8717, 45367, 3224, 11933, 1829, 8592, 39896, 30544, 8717, 45367, 3224, 3714, 43020, 7251, 30544, 22633, 1776, 401, 34051, 11331, 26108, 3660, 6055, 7649, 10632, 9122, 5016, 12610, 8717, 45367, 6055, 7649, 1829, 32771, 13546, 2423, 22633, 1776, 401, 9122, 33911, 4724, 13546, 2655, 3555, 9566, 27188, 9307, 1829, 5551, 3794, 3215, 20449, 33251, 5016, 12610, 4724, 9403, 6086, 538, 9403, 6086, 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{"start": 762.13, "end": 762.53, "word": " بيدوب", "probability": 0.8681640625}, {"start": 762.53, "end": 762.79, "word": " فيه", "probability": 0.989013671875}, {"start": 762.79, "end": 763.31, "word": " إذا", "probability": 0.7568359375}, {"start": 763.31, "end": 763.85, "word": " جبتله", "probability": 0.949462890625}, {"start": 763.85, "end": 764.23, "word": " polar", "probability": 0.978515625}, {"start": 764.23, "end": 764.49, "word": " مش", "probability": 0.962890625}, {"start": 764.49, "end": 764.89, "word": " هيدوب", "probability": 0.8317057291666666}, {"start": 764.89, "end": 765.23, "word": " فيه", "probability": 0.995849609375}, {"start": 765.23, "end": 765.79, "word": " احنا", "probability": 0.8611653645833334}, {"start": 765.79, "end": 765.95, "word": " في", "probability": 0.95703125}, {"start": 765.95, "end": 766.23, "word": " هذا", "probability": 0.97021484375}, {"start": 766.23, "end": 766.83, "word": " الفحص", "probability": 0.91943359375}, {"start": 766.83, "end": 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هل اللبد هيدوب ولا مش هيدوب هنلاحظ ان اللبد بدوب فيهم كله تم هذا ال distill water لأنه عبارة عن boiler و اللبد non boiler فبيدوبش فيه", "tokens": [2407, 8717, 3215, 37746, 13672, 3555, 3215, 8978, 16095, 2423, 11622, 36081, 50113, 15040, 8717, 7435, 1829, 3555, 30767, 36081, 8717, 3215, 37746, 3224, 8978, 9673, 16606, 8717, 8592, 38688, 16472, 3224, 47525, 37746, 49429, 47525, 37746, 8592, 39182, 9957, 47525, 8315, 8717, 7435, 1829, 3555, 6024, 110, 36081, 4032, 8717, 3215, 37746, 3224, 8978, 1975, 1829, 3224, 8717, 8592, 38688, 8032, 1211, 39896, 3215, 37746, 49429, 37893, 39896, 3215, 37746, 39182, 9957, 8717, 7435, 1829, 3555, 28242, 13063, 38688, 9640, 3555, 4032, 8717, 3215, 37746, 3224, 8978, 3224, 8717, 8592, 38688, 8032, 1211, 13672, 3555, 3215, 39896, 3215, 37746, 49429, 37893, 39896, 3215, 37746, 8032, 1863, 15040, 5016, 19913, 16472, 13672, 3555, 3215, 47525, 37746, 8978, 16095, 28242, 3224, 46811, 23758, 2423, 42923, 1281, 5296, 33456, 3224, 6225, 3555, 9640, 3660, 18871, 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811.57, "end": 811.81, "word": " ومش", "probability": 0.8138020833333334}, {"start": 811.81, "end": 812.07, "word": " هيدوب", "probability": 0.96923828125}, {"start": 812.07, "end": 812.21, "word": " في", "probability": 0.98291015625}, {"start": 812.21, "end": 812.97, "word": " الماء،", "probability": 0.82080078125}, {"start": 812.97, "end": 813.15, "word": " بناء", "probability": 0.961669921875}, {"start": 813.15, "end": 813.37, "word": " على", "probability": 0.91845703125}, {"start": 813.37, "end": 813.65, "word": " أي", "probability": 0.9580078125}, {"start": 813.65, "end": 814.69, "word": " مبدأ،", "probability": 0.870849609375}, {"start": 814.69, "end": 814.97, "word": " حكينا", "probability": 0.9454345703125}, {"start": 814.97, "end": 815.31, "word": " بناء", "probability": 0.945068359375}, {"start": 815.31, "end": 815.57, "word": " على", "probability": 0.8955078125}, {"start": 815.57, "end": 816.09, "word": " مبدأ", "probability": 0.9876302083333334}, {"start": 816.09, "end": 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"temperature": 1.0}, {"id": 34, "seek": 85883, "start": 835.83, "end": 858.83, "text": "الصليبوليتي بتعتمد على ال polarity الدوبانية بتعتمد على القطمية طيب ايش ال reagent اللى بدنا نستخدمه في الفحص هاد؟ بدنا نستخدم دهون، بدنا نستخدم زيت الزتون و الفات اللى هى ال butter أو الفات اللى هى الزبدة المواد اللى بدنا ندوب فيها ال solvent هي بدنا نستخدم إيثانول و chloroform و ether و water", "tokens": [6027, 9381, 20292, 3555, 12610, 1829, 31371, 39894, 34268, 2304, 3215, 15844, 2423, 12367, 507, 32748, 37746, 7649, 10632, 39894, 34268, 2304, 3215, 15844, 25062, 9566, 2304, 10632, 23032, 1829, 3555, 1975, 1829, 8592, 2423, 26949, 317, 13672, 7578, 47525, 8315, 8717, 14851, 9778, 40448, 3224, 8978, 27188, 5016, 9381, 8032, 18513, 22807, 47525, 8315, 8717, 14851, 9778, 40448, 11778, 3224, 11536, 12399, 47525, 8315, 8717, 14851, 9778, 40448, 30767, 36081, 6024, 110, 2655, 11536, 4032, 27188, 9307, 13672, 7578, 8032, 7578, 2423, 5517, 34051, 27188, 9307, 13672, 7578, 8032, 7578, 6024, 110, 3555, 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853.29, "end": 853.57, "word": " هي", "probability": 0.77978515625}, {"start": 853.57, "end": 853.89, "word": " بدنا", "probability": 0.691650390625}, {"start": 853.89, "end": 854.31, "word": " نستخدم", "probability": 0.996337890625}, {"start": 854.31, "end": 855.09, "word": " إيثانول", "probability": 0.7343017578125}, {"start": 855.09, "end": 855.51, "word": " و", "probability": 0.94873046875}, {"start": 855.51, "end": 856.97, "word": " chloroform", "probability": 0.5828450520833334}, {"start": 856.97, "end": 857.47, "word": " و", "probability": 0.96923828125}, {"start": 857.47, "end": 857.97, "word": " ether", "probability": 0.712890625}, {"start": 857.97, "end": 858.39, "word": " و", "probability": 0.982421875}, {"start": 858.39, "end": 858.83, "word": " water", "probability": 0.95458984375}], "temperature": 1.0}, {"id": 35, "seek": 88141, "start": 860.69, "end": 881.41, "text": "كيف بدي أشتغل الفحص؟ بدي أجيب أربع تيوبات واحدة تشتغل فيها ديستل واتر واحدة إيثر واحدة كلوروفور واحدة إيثانور تمام؟ بدي أجيب لتيوب الأول تاعة الديستل واتر بدي أحط فيها drop oil drop من الزيت على واحد مل من الديستل واتر بدي أجيب لتيوب الأول", "tokens": [4117, 33911, 4724, 16254, 5551, 8592, 2655, 17082, 1211, 27188, 5016, 9381, 22807, 4724, 16254, 5551, 7435, 1829, 3555, 5551, 25513, 3615, 6055, 1829, 37746, 9307, 36764, 24401, 3660, 6055, 8592, 2655, 17082, 1211, 8978, 11296, 11778, 1829, 14851, 1211, 4032, 9307, 2288, 36764, 24401, 3660, 11933, 1829, 49115, 36764, 24401, 3660, 28242, 13063, 38688, 13063, 36764, 24401, 3660, 11933, 1829, 12984, 7649, 13063, 46811, 10943, 22807, 4724, 16254, 5551, 7435, 1829, 3555, 5296, 31371, 37746, 16247, 12610, 6055, 995, 27884, 2423, 16254, 14851, 1211, 4032, 9307, 2288, 4724, 16254, 5551, 5016, 9566, 8978, 11296, 3270, 3184, 3270, 9154, 6024, 110, 36081, 15844, 36764, 24401, 3714, 1211, 9154, 2423, 16254, 14851, 1211, 4032, 9307, 2288, 4724, 16254, 5551, 7435, 1829, 3555, 5296, 31371, 37746, 16247, 12610], "avg_logprob": -0.17531987836980445, "compression_ratio": 2.274193548387097, "no_speech_prob": 0.0, "words": [{"start": 860.69, "end": 860.99, "word": "كيف", "probability": 0.878173828125}, {"start": 860.99, "end": 861.17, "word": " بدي", "probability": 0.5963134765625}, {"start": 861.17, "end": 861.59, "word": " أشتغل", "probability": 0.869140625}, {"start": 861.59, "end": 862.49, "word": " الفحص؟", "probability": 0.8748779296875}, {"start": 862.49, "end": 862.69, "word": " بدي", "probability": 0.936767578125}, {"start": 862.69, "end": 863.11, "word": " أجيب", "probability": 0.97509765625}, {"start": 863.11, "end": 863.47, "word": " أربع", "probability": 0.7418619791666666}, {"start": 863.47, "end": 864.25, "word": " تيوبات", "probability": 0.869384765625}, {"start": 864.25, "end": 864.79, "word": " واحدة", "probability": 0.9072265625}, {"start": 864.79, "end": 865.19, "word": " تشتغل", "probability": 0.97216796875}, {"start": 865.19, "end": 865.37, "word": " فيها", "probability": 0.9541015625}, {"start": 865.37, "end": 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soluble او insoluble ال cube التانية بدي أحط عليها drop من ال oil على واحد من ال ether بدي أحط منها drop من الزيت على واحد من ال ether و أشوف بيدوب ولا بيدوبش", "tokens": [10721, 5016, 9566, 8978, 11296, 3270, 9154, 2423, 3184, 8717, 47432, 3660, 9154, 6024, 110, 36081, 15844, 36764, 24401, 9154, 2423, 42923, 1281, 4032, 5551, 25957, 1211, 2890, 5551, 8592, 38688, 8032, 1211, 39896, 30544, 11778, 16373, 3555, 49429, 32771, 13546, 11778, 16373, 3555, 24807, 638, 1975, 2407, 1028, 39754, 638, 2423, 13728, 16712, 7649, 10632, 4724, 16254, 5551, 5016, 9566, 25894, 11296, 3270, 9154, 2423, 3184, 15844, 36764, 24401, 9154, 2423, 37096, 4724, 16254, 5551, 5016, 9566, 9154, 11296, 3270, 9154, 6024, 110, 36081, 15844, 36764, 24401, 9154, 2423, 37096, 4032, 5551, 8592, 38688, 4724, 25708, 37746, 49429, 4724, 25708, 37746, 8592], "avg_logprob": -0.17127403187064025, "compression_ratio": 2.0145631067961167, "no_speech_prob": 1.1920928955078125e-07, "words": [{"start": 881.7, "end": 882.2, "word": 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"probability": 0.78668212890625}, {"start": 1427.16, "end": 1427.86, "word": " إيش", "probability": 0.730224609375}, {"start": 1427.86, "end": 1428.22, "word": " العامل", "probability": 0.9903971354166666}, {"start": 1428.22, "end": 1428.66, "word": " المستحلب", "probability": 0.979248046875}, {"start": 1428.66, "end": 1428.8, "word": " اللي", "probability": 0.96435546875}, {"start": 1428.8, "end": 1428.98, "word": " بدي", "probability": 0.914794921875}, {"start": 1428.98, "end": 1429.64, "word": " أستخدمه", "probability": 0.899609375}, {"start": 1429.64, "end": 1430.02, "word": " هو", "probability": 0.2298583984375}, {"start": 1430.02, "end": 1430.8, "word": " نصف", "probability": 0.7771809895833334}, {"start": 1430.8, "end": 1431.4, "word": " المية", "probability": 0.6689453125}], "temperature": 1.0}, {"id": 60, "seek": 145492, "start": 1432.62, "end": 1454.92, "text": "NA2CO3 Sodium Bicarbonate هذا إيش وظيفته في هذا الفحص وظيفته Emulsifying Agent عامل مستحل وبدنا نشتغل الفحص على زيت زتون طبيعي Natural Olive Oil وعلى Rancid Olive Oil على زيت زتون طبيعي وزيت الزتون المتزنخ", "tokens": [5321, 17, 12322, 18, 42059, 2197, 363, 7953, 4351, 473, 23758, 11933, 1829, 8592, 4032, 19913, 33911, 47395, 8978, 23758, 27188, 5016, 9381, 4032, 19913, 33911, 47395, 3968, 9468, 5489, 27174, 6225, 10943, 1211, 3714, 14851, 5016, 1211, 4032, 44510, 8315, 8717, 8592, 2655, 17082, 1211, 27188, 5016, 9381, 15844, 30767, 36081, 30767, 2655, 11536, 23032, 21292, 3615, 1829, 20137, 35741, 23545, 4032, 3615, 23942, 497, 4463, 327, 35741, 23545, 15844, 30767, 36081, 30767, 2655, 11536, 23032, 21292, 3615, 1829, 4032, 11622, 36081, 6024, 110, 2655, 11536, 9673, 2655, 11622, 1863, 9778], "avg_logprob": -0.13667674282545683, "compression_ratio": 1.5477386934673367, "no_speech_prob": 1.1920928955078125e-07, "words": [{"start": 1432.62, "end": 1433.62, "word": "NA2CO3", "probability": 0.8336181640625}, {"start": 1433.62, "end": 1434.32, "word": " Sodium", "probability": 0.734619140625}, {"start": 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بيحكي لل natural olive oil اللي هو زيت الزيتون الطبيعي بيكون جديد، بيكون لسه مش ساير عليه عمليات أكسدة، بيكون سايرله بس partial oxidation عمليات أكسدة بسيطة جزئية", "tokens": [16296, 15981, 3184, 30767, 36081, 6024, 110, 36081, 11536, 41950, 21292, 3615, 1829, 4032, 8717, 14851, 9778, 40448, 367, 4463, 327, 15981, 3184, 13672, 1829, 31439, 30767, 36081, 6024, 110, 36081, 11536, 9673, 2655, 11622, 1863, 9778, 1975, 1829, 8592, 27188, 2288, 4587, 49374, 2423, 9307, 1863, 9957, 22807, 4724, 1829, 5016, 4117, 1829, 24976, 3303, 15981, 3184, 13672, 1829, 31439, 30767, 36081, 6024, 110, 36081, 11536, 41950, 21292, 3615, 1829, 4724, 1829, 30544, 10874, 16254, 3215, 12399, 4724, 1829, 30544, 5296, 3794, 3224, 37893, 8608, 995, 13546, 47356, 6225, 2304, 20292, 9307, 5551, 4117, 3794, 41891, 12399, 4724, 1829, 30544, 8608, 995, 13546, 43761, 4724, 3794, 14641, 36767, 6225, 2304, 20292, 9307, 5551, 4117, 3794, 41891, 4724, 3794, 1829, 9566, 3660, 10874, 11622, 19986, 10632], "avg_logprob": 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"word": " natural", "probability": 0.57666015625}, {"start": 1464.74, "end": 1465.1, "word": " olive", "probability": 0.85107421875}, {"start": 1465.1, "end": 1465.4, "word": " oil", "probability": 0.98583984375}, {"start": 1465.4, "end": 1465.5, "word": " اللي", "probability": 0.978759765625}, {"start": 1465.5, "end": 1465.66, "word": " هو", "probability": 0.9775390625}, {"start": 1465.66, "end": 1465.84, "word": " زيت", "probability": 0.989501953125}, {"start": 1465.84, "end": 1466.28, "word": " الزيتون", "probability": 0.981201171875}, {"start": 1466.28, "end": 1467.04, "word": " الطبيعي", "probability": 0.9796142578125}, {"start": 1467.04, "end": 1467.8, "word": " بيكون", "probability": 0.8644205729166666}, {"start": 1467.8, "end": 1468.4, "word": " جديد،", "probability": 0.886474609375}, {"start": 1468.4, "end": 1468.7, "word": " بيكون", "probability": 0.9737955729166666}, {"start": 1468.7, "end": 1469.0, "word": " لسه", "probability": 0.9388020833333334}, {"start": 1469.0, "end": 1469.18, "word": " مش", "probability": 0.9921875}, {"start": 1469.18, "end": 1469.5, "word": " ساير", "probability": 0.8533528645833334}, {"start": 1469.5, "end": 1469.74, "word": " عليه", "probability": 0.9306640625}, {"start": 1469.74, "end": 1470.24, "word": " عمليات", "probability": 0.8751220703125}, {"start": 1470.24, "end": 1471.2, "word": " أكسدة،", "probability": 0.90224609375}, {"start": 1471.2, "end": 1471.54, "word": " بيكون", "probability": 0.98583984375}, {"start": 1471.54, "end": 1471.98, "word": " سايرله", "probability": 0.9217529296875}, {"start": 1471.98, "end": 1472.3, "word": " بس", "probability": 0.972412109375}, {"start": 1472.3, "end": 1472.8, "word": " partial", "probability": 0.5458984375}, {"start": 1472.8, "end": 1473.46, "word": " oxidation", "probability": 0.93896484375}, {"start": 1473.46, "end": 1473.96, "word": " عمليات", "probability": 0.8621826171875}, {"start": 1473.96, "end": 1474.38, "word": " أكسدة", "probability": 0.9920654296875}, {"start": 1474.38, "end": 1475.0, "word": " بسيطة", "probability": 0.99404296875}, {"start": 1475.0, "end": 1475.7, "word": " جزئية", "probability": 0.9388427734375}], "temperature": 1.0}, {"id": 62, "seek": 150273, "start": 1476.31, "end": 1502.73, "text": "لما تأكسدى انتهى أي جزاء تأكسدى هذا الزيت بينتج عنه تكسير للفاتي أسد بتتكسر بتتفكك عن بعضها الفاتي أسد وبتتحرر فبيصير عندى فيه free fatty acid في ال natural olive oil بيكون partially ال oxidation عمليات أكسدة جزئية بسيطة فكمية الفاتي أسد الحرة بتكون قليلة يحتوي على few of free fatty acid", "tokens": [1211, 15042, 6055, 10721, 4117, 3794, 3215, 7578, 16472, 47395, 7578, 36632, 10874, 11622, 16606, 6055, 10721, 4117, 3794, 3215, 7578, 23758, 6024, 110, 36081, 4724, 9957, 2655, 7435, 18871, 3224, 6055, 4117, 3794, 13546, 24976, 5172, 9307, 1829, 5551, 3794, 3215, 39894, 2655, 4117, 3794, 2288, 39894, 2655, 5172, 4117, 4117, 18871, 45030, 11242, 11296, 27188, 9307, 1829, 5551, 3794, 3215, 46599, 2655, 2655, 5016, 2288, 2288, 6156, 3555, 1829, 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"end": 1490.99, "word": " olive", "probability": 0.75048828125}, {"start": 1490.99, "end": 1491.45, "word": " oil", "probability": 0.98583984375}, {"start": 1491.45, "end": 1491.85, "word": " بيكون", "probability": 0.9313151041666666}, {"start": 1491.85, "end": 1492.33, "word": " partially", "probability": 0.2305908203125}, {"start": 1492.33, "end": 1492.59, "word": " ال", "probability": 0.52685546875}, {"start": 1492.59, "end": 1493.23, "word": " oxidation", "probability": 0.7763671875}, {"start": 1493.23, "end": 1494.27, "word": " عمليات", "probability": 0.9329833984375}, {"start": 1494.27, "end": 1494.85, "word": " أكسدة", "probability": 0.977783203125}, {"start": 1494.85, "end": 1495.55, "word": " جزئية", "probability": 0.9473876953125}, {"start": 1495.55, "end": 1496.27, "word": " بسيطة", "probability": 0.984375}, {"start": 1496.27, "end": 1497.19, "word": " فكمية", "probability": 0.9485677083333334}, {"start": 1497.19, "end": 1497.69, "word": " الفاتي", "probability": 0.9267578125}, {"start": 1497.69, "end": 1497.99, "word": " أسد", "probability": 0.9910481770833334}, {"start": 1497.99, "end": 1498.39, "word": " الحرة", "probability": 0.933349609375}, {"start": 1498.39, "end": 1498.77, "word": " بتكون", "probability": 0.92578125}, {"start": 1498.77, "end": 1499.35, "word": " قليلة", "probability": 0.9716796875}, {"start": 1499.35, "end": 1499.91, "word": " يحتوي", "probability": 0.76904296875}, {"start": 1499.91, "end": 1500.13, "word": " على", "probability": 0.93896484375}, {"start": 1500.13, "end": 1500.59, "word": " few", "probability": 0.83837890625}, {"start": 1500.59, "end": 1501.29, "word": " of", "probability": 0.962890625}, {"start": 1501.29, "end": 1501.81, "word": " free", "probability": 0.7421875}, {"start": 1501.81, "end": 1502.37, "word": " fatty", "probability": 0.93115234375}, {"start": 1502.37, "end": 1502.73, "word": " acid", "probability": 0.9072265625}], "temperature": 1.0}, {"id": 63, "seek": 152692, "start": 1503.32, "end": 1526.92, "text": "لكن في الـ Rancid Olive Oil في زيت الزتون المتزنخ اللي هو مجلة فيه أكتر من مرة عاملينه مستخدمينه أكتر من مرة بيكون صاير عليه Complete Oxidation عمليات أكسدة كاملة بتأدي إلى تحرير جميع الفاتي أسد فبيكون عندنا many of free fatty acid الفاتي أسد بتكون الـ free الحرة بتكون بشكل أكبر", "tokens": [1211, 19452, 8978, 2423, 39184, 497, 4463, 327, 35741, 23545, 8978, 30767, 36081, 6024, 110, 2655, 11536, 9673, 2655, 11622, 1863, 9778, 13672, 1829, 31439, 3714, 7435, 37977, 8978, 3224, 5551, 4117, 2655, 2288, 9154, 3714, 25720, 6225, 10943, 1211, 9957, 3224, 3714, 14851, 9778, 40448, 9957, 3224, 5551, 4117, 2655, 2288, 9154, 3714, 25720, 4724, 1829, 30544, 20328, 995, 13546, 47356, 34687, 16489, 327, 399, 6225, 2304, 20292, 9307, 5551, 4117, 3794, 41891, 9122, 10943, 37977, 39894, 10721, 16254, 30731, 6055, 5016, 2288, 13546, 10874, 2304, 40228, 27188, 9307, 1829, 5551, 3794, 3215, 6156, 21292, 30544, 43242, 8315, 867, 295, 1737, 24898, 8258, 27188, 9307, 1829, 5551, 3794, 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2273.25, "end": 2273.93, "word": " تكون", "probability": 0.898681640625}, {"start": 2273.93, "end": 2274.69, "word": " لتيوبات", "probability": 0.7220458984375}, {"start": 2274.69, "end": 2275.45, "word": " جافة", "probability": 0.98095703125}, {"start": 2275.45, "end": 2276.05, "word": " ضروري", "probability": 0.9285888671875}, {"start": 2276.05, "end": 2276.63, "word": " لتيوبات", "probability": 0.9561767578125}, {"start": 2276.63, "end": 2276.95, "word": " تكون", "probability": 0.9599609375}, {"start": 2276.95, "end": 2277.53, "word": " جافة", "probability": 0.9944661458333334}, {"start": 2277.53, "end": 2278.03, "word": " لأنه", "probability": 0.5602213541666666}, {"start": 2278.03, "end": 2278.21, "word": " هذا", "probability": 0.85693359375}, {"start": 2278.21, "end": 2278.71, "word": " حكينا", "probability": 0.8853759765625}, {"start": 2278.71, "end": 2278.93, "word": " أنه", "probability": 0.5645751953125}, {"start": 2278.93, "end": 2279.19, "word": " highly", "probability": 0.207763671875}, {"start": 2279.19, "end": 2280.05, "word": " hygroscopic", "probability": 0.8870849609375}, {"start": 2280.05, "end": 2280.83, "word": " حساس", "probability": 0.9733072916666666}, {"start": 2280.83, "end": 2281.89, "word": " للرطوبة", "probability": 0.89375}, {"start": 2281.89, "end": 2282.13, "word": " لأن", "probability": 0.845703125}, {"start": 2282.13, "end": 2282.51, "word": " أصلا", "probability": 0.7291666666666666}, {"start": 2282.51, "end": 2282.95, "word": " المبدأ", "probability": 0.8671875}, {"start": 2282.95, "end": 2283.33, "word": " عامله", "probability": 0.9783528645833334}, {"start": 2283.33, "end": 2283.53, "word": " أنه", "probability": 0.85546875}, {"start": 2283.53, "end": 2283.77, "word": " بده", "probability": 0.791015625}, {"start": 2283.77, "end": 2284.37, "word": " جزيئين", "probability": 0.868359375}, {"start": 2284.37, "end": 2284.49, "word": " ما", "probability": 0.6279296875}, {"start": 2284.49, "end": 2284.93, "word": " ينزع", "probability": 0.9464111328125}, {"start": 2284.93, "end": 2285.45, "word": " جزيئين", "probability": 0.98544921875}, {"start": 2285.45, "end": 2285.63, "word": " ما", "probability": 0.9228515625}], "temperature": 1.0}, {"id": 99, "seek": 230585, "start": 2285.87, "end": 2305.85, "text": "إذا التيوب فيها مي هياخد المي الموجودة في التيوب مش هيشتغل على الكوليسترول إذا موجود انما هيشتغل على المي اللي في التيوب بدل الكوليسترول هيك بنكون خلصنا هذا المعمل، المعمل الليبد ان شاء الله نشتغل عملي فيه المعمل، مطلوب منكم", "tokens": [28814, 15730, 2423, 31371, 37746, 8978, 11296, 3714, 1829, 39896, 47283, 3215, 9673, 1829, 9673, 29245, 23328, 3660, 8978, 2423, 31371, 37746, 37893, 39896, 8592, 2655, 17082, 1211, 15844, 33251, 12610, 1829, 14851, 2288, 12610, 11933, 15730, 3714, 29245, 23328, 16472, 15042, 39896, 8592, 2655, 17082, 1211, 15844, 9673, 1829, 13672, 1829, 8978, 2423, 31371, 37746, 47525, 1211, 33251, 12610, 1829, 14851, 2288, 12610, 39896, 4117, 44945, 30544, 16490, 1211, 9381, 8315, 23758, 9673, 25957, 1211, 12399, 9673, 25957, 1211, 13672, 1829, 44510, 16472, 13412, 16606, 21984, 8717, 8592, 2655, 17082, 1211, 6225, 2304, 20292, 8978, 3224, 9673, 25957, 1211, 12399, 3714, 9566, 1211, 37746, 9154, 24793], "avg_logprob": -0.20399305941881957, "compression_ratio": 2.2849162011173183, "no_speech_prob": 1.3709068298339844e-06, "words": [{"start": 2285.87, "end": 2286.31, "word": "إذا", "probability": 0.6722412109375}, {"start": 2286.31, "end": 2286.71, "word": " التيوب", "probability": 0.4547526041666667}, {"start": 2286.71, "end": 2287.05, "word": " فيها", "probability": 0.927734375}, {"start": 2287.05, "end": 2287.35, "word": " مي", "probability": 0.869140625}, {"start": 2287.35, "end": 2288.25, "word": " هياخد", "probability": 0.5589192708333334}, {"start": 2288.25, "end": 2289.09, "word": " المي", "probability": 0.836669921875}, {"start": 2289.09, "end": 2289.63, "word": " الموجودة", "probability": 0.8868408203125}, {"start": 2289.63, "end": 2289.71, "word": " في", "probability": 0.95458984375}, {"start": 2289.71, "end": 2290.13, "word": " التيوب", "probability": 0.8447265625}, {"start": 2290.13, "end": 2290.35, "word": " مش", "probability": 0.20361328125}, {"start": 2290.35, "end": 2291.05, "word": " هيشتغل", "probability": 0.9197265625}, {"start": 2291.05, "end": 2291.25, "word": " على", "probability": 0.87890625}, {"start": 2291.25, "end": 2292.13, "word": " الكوليسترول", "probability": 0.88720703125}, {"start": 2292.13, "end": 2292.51, "word": " إذا", "probability": 0.897216796875}, {"start": 2292.51, "end": 2293.23, "word": " موجود", "probability": 0.96484375}, {"start": 2293.23, "end": 2294.03, "word": " انما", "probability": 0.570068359375}, {"start": 2294.03, "end": 2294.59, "word": " هيشتغل", "probability": 0.935546875}, {"start": 2294.59, "end": 2294.79, "word": " على", "probability": 0.88623046875}, {"start": 2294.79, "end": 2295.21, "word": " المي", "probability": 0.932373046875}, {"start": 2295.21, "end": 2295.39, "word": " اللي", "probability": 0.770751953125}, {"start": 2295.39, "end": 2295.51, "word": " في", "probability": 0.9677734375}, {"start": 2295.51, "end": 2296.21, "word": " التيوب", "probability": 0.9345703125}, {"start": 2296.21, "end": 2296.83, "word": " بدل", "probability": 0.97216796875}, {"start": 2296.83, "end": 2297.73, "word": " الكوليسترول", "probability": 0.96826171875}, {"start": 2297.73, "end": 2299.53, "word": " هيك", "probability": 0.8076171875}, {"start": 2299.53, "end": 2299.87, "word": " بنكون", "probability": 0.919921875}, {"start": 2299.87, "end": 2300.47, "word": " خلصنا", "probability": 0.9716796875}, {"start": 2300.47, "end": 2300.73, "word": " هذا", "probability": 0.501953125}, {"start": 2300.73, "end": 2301.41, "word": " المعمل،", "probability": 0.8876953125}, {"start": 2301.41, "end": 2301.71, "word": " المعمل", "probability": 0.8115234375}, {"start": 2301.71, "end": 2302.17, "word": " الليبد", "probability": 0.73876953125}, {"start": 2302.17, "end": 2302.67, "word": " ان", "probability": 0.57421875}, {"start": 2302.67, "end": 2302.83, "word": " شاء", "probability": 0.958251953125}, {"start": 2302.83, "end": 2302.89, "word": " الله", "probability": 0.9638671875}, {"start": 2302.89, "end": 2303.31, "word": " نشتغل", "probability": 0.95546875}, {"start": 2303.31, "end": 2303.73, "word": " عملي", "probability": 0.8056640625}, {"start": 2303.73, "end": 2304.05, "word": " فيه", "probability": 0.979248046875}, {"start": 2304.05, "end": 2305.01, "word": " المعمل،", "probability": 0.9056396484375}, {"start": 2305.01, "end": 2305.45, "word": " مطلوب", "probability": 0.92431640625}, {"start": 2305.45, "end": 2305.85, "word": " منكم", "probability": 0.8779296875}], "temperature": 1.0}, {"id": 100, "seek": 231708, "start": 2306.14, "end": 2317.08, "text": "تكتبولي ال principle لكل فحص على شكل معادلات و اللي مش معادلة تكتبولي المبدأ برضه يعطيكم العافية", "tokens": [2655, 4117, 2655, 3555, 12610, 1829, 2423, 8665, 5296, 28820, 6156, 5016, 9381, 15844, 13412, 28820, 20449, 18513, 1211, 9307, 4032, 13672, 1829, 37893, 20449, 18513, 37977, 6055, 4117, 2655, 3555, 12610, 1829, 9673, 44510, 10721, 4724, 43042, 3224, 37495, 9566, 1829, 24793, 18863, 31845, 10632], "avg_logprob": -0.07849900012320661, "compression_ratio": 1.403361344537815, "no_speech_prob": 0.0, "words": [{"start": 2306.14, "end": 2306.98, "word": "تكتبولي", "probability": 0.9536946614583334}, {"start": 2306.98, "end": 2307.1, "word": " ال", "probability": 0.95849609375}, {"start": 2307.1, "end": 2307.82, "word": " principle", "probability": 0.7021484375}, {"start": 2307.82, "end": 2308.74, "word": " لكل", "probability": 0.979248046875}, {"start": 2308.74, "end": 2309.48, "word": " فحص", "probability": 0.9622395833333334}, {"start": 2309.48, "end": 2310.08, "word": " على", "probability": 0.8798828125}, {"start": 2310.08, "end": 2310.68, "word": " شكل", "probability": 0.9814453125}, {"start": 2310.68, "end": 2312.04, "word": " معادلات", "probability": 0.99365234375}, {"start": 2312.04, "end": 2312.64, "word": " و", "probability": 0.74267578125}, {"start": 2312.64, "end": 2312.76, "word": " اللي", "probability": 0.7054443359375}, {"start": 2312.76, "end": 2313.04, "word": " مش", "probability": 0.994140625}, {"start": 2313.04, "end": 2313.58, "word": " معادلة", "probability": 0.9755859375}, {"start": 2313.58, "end": 2314.14, "word": " تكتبولي", "probability": 0.8985188802083334}, {"start": 2314.14, "end": 2314.62, "word": " المبدأ", "probability": 0.9676106770833334}, {"start": 2314.62, "end": 2315.64, "word": " برضه", "probability": 0.9881184895833334}, {"start": 2315.64, "end": 2316.52, "word": " يعطيكم", "probability": 0.939697265625}, {"start": 2316.52, "end": 2317.08, "word": " العافية", "probability": 0.9567057291666666}], "temperature": 1.0}], "language": "ar", "language_probability": 1.0, "duration": 2318.4428125, "duration_after_vad": 2256.7368749999937}
\ No newline at end of file
diff --git a/PL9fwy3NUQKwZ1Q7zOg1ObgufPMjRI3WY1/VOnoEDYqP7I_raw.srt b/PL9fwy3NUQKwZ1Q7zOg1ObgufPMjRI3WY1/VOnoEDYqP7I_raw.srt
new file mode 100644
index 0000000000000000000000000000000000000000..2c39f6777633a96e52b33e56b6e7918cdd4c8e3e
--- /dev/null
+++ b/PL9fwy3NUQKwZ1Q7zOg1ObgufPMjRI3WY1/VOnoEDYqP7I_raw.srt
@@ -0,0 +1,2104 @@
+1
+00:00:00,770 --> 00:00:04,710
+اليوم ان شاء الله بدنا نحكي في موضوع جديد اللي هو
+
+2
+00:00:04,710 --> 00:00:09,590
+موضوع الدهون بدنا نحكي عن ال libid عن الدهون نتعرف
+
+3
+00:00:09,590 --> 00:00:16,030
+عليها وعلى فحوصات للكشف عن الدهون في البداية بدنا
+
+4
+00:00:16,030 --> 00:00:21,550
+نحكي تعريف ال libid ال libid هي group of naturally
+
+5
+00:00:21,550 --> 00:00:25,030
+occurring substance consisting of higher fatty
+
+6
+00:00:25,030 --> 00:00:28,710
+acid وهي naturally occurring substance أو compound
+
+7
+00:00:30,320 --> 00:00:35,920
+مركبات أو مجموعة من المكو .. من المركبات تتكون من
+
+8
+00:00:35,920 --> 00:00:41,060
+نسبة عالية من الأحماض الدهنية، لذان الدهون هي
+
+9
+00:00:41,060 --> 00:00:46,760
+عبارة عن مركبات تحتوي على نسبة عالية من الأحماض
+
+10
+00:00:46,760 --> 00:00:50,440
+الدهنية، من ال fatty acid، من الأحماض الدهنية، وهي
+
+11
+00:00:50,440 --> 00:00:53,200
+in the generally occurring substance هي مواد
+
+12
+00:00:53,200 --> 00:00:59,020
+موجودة طبيعيا في طعامنا اليومOrganic substance
+
+13
+00:00:59,020 --> 00:01:02,140
+relatively insoluble in water but soluble in
+
+14
+00:01:02,140 --> 00:01:05,780
+organic solvent بيحكي لإن اللي بتطبيعتها هي
+
+15
+00:01:05,780 --> 00:01:11,780
+organic هي مواد عضوية هي non-polar هي مواد عضوية
+
+16
+00:01:11,780 --> 00:01:16,580
+non-polarلا تدوب في الماء مدامها non-polar إذا مش
+
+17
+00:01:16,580 --> 00:01:20,860
+هتدوب في الماء في الماء وين هتدوب؟ بتدوب بيحكيلي
+
+18
+00:01:20,860 --> 00:01:25,400
+في ال organic solvent في اللي بيشبهها المبدأ like,
+
+19
+00:01:25,500 --> 00:01:30,580
+dissolve like المتشابهات دوتيب بعضها فهي بتدوب في
+
+20
+00:01:30,580 --> 00:01:34,280
+المواد العضوية اللي زيها زي مين مواد عضوية بتدوب
+
+21
+00:01:34,280 --> 00:01:39,430
+فيها ال lipid؟ زي chloroform, ether, benzeneعلى
+
+22
+00:01:39,430 --> 00:01:45,250
+مبدأ like dissolve like إن المتشابهات تديب بعضها
+
+23
+00:01:45,250 --> 00:01:50,790
+function of libid لو جينا نتعرف على وظائف اللبد
+
+24
+00:01:50,790 --> 00:01:55,210
+أول وظيفة حكينا عنها في أول معمل إنها storage form
+
+25
+00:01:55,210 --> 00:02:00,510
+of energy هي مخزن الطاقة ال libid تعتبر مخزن
+
+26
+00:02:00,510 --> 00:02:04,510
+الطاقة الوظيفة التانية هي structural component of
+
+27
+00:02:04,510 --> 00:02:09,460
+cell membraneهي بتدخل في تركيب ال cell membrane في
+
+28
+00:02:09,460 --> 00:02:14,860
+تركيب الغلاف الخلوي الجدار الخلوي بتدخل فيه تركيبه
+
+29
+00:02:14,860 --> 00:02:19,140
+اللبن تلاتة هي precursor of many steroid hormone
+
+30
+00:02:19,140 --> 00:02:25,380
+as estrogen و vitamin D هي اللبن الأساسية في تركيب
+
+31
+00:02:25,380 --> 00:02:29,000
+الهرمونات الاسترويدية اللي هي نفسها ال sex hormone
+
+32
+00:02:29,000 --> 00:02:33,600
+زي الاستروجين و vitamin D اللي برضه هي بتدخل فيه
+
+33
+00:02:33,600 --> 00:02:39,160
+تركيبهprotection of internal organs تعمل على حماية
+
+34
+00:02:39,160 --> 00:02:44,180
+الأعضاء الداخلية الدهون تعمل على حماية الأعضاء
+
+35
+00:02:44,180 --> 00:02:44,980
+الداخلية
+
+36
+00:02:47,330 --> 00:02:51,990
+هنا فيه إنه رسمة ل cell membrane بدي واضحني إنه
+
+37
+00:02:51,990 --> 00:02:54,730
+أغلب مكونات ال cell membrane هي عبارة عن lipid
+
+38
+00:02:54,730 --> 00:02:58,210
+يعني outside of cell اللي خارج الخلية فيها
+
+39
+00:02:58,210 --> 00:03:01,550
+نسفينجومايليين هذا lipid ال glycolipid برضه lipid
+
+40
+00:03:01,550 --> 00:03:05,670
+و ال phosphatigolين فيها ناكلسترول كل هذه المكونات
+
+41
+00:03:05,670 --> 00:03:09,210
+عبارة عن lipid فأغلب مكونات ال cell membrane هي
+
+42
+00:03:09,210 --> 00:03:14,290
+عبارة عن lipid فهو التركيبة الأساسية ل ال cell
+
+43
+00:03:14,290 --> 00:03:14,830
+membrane
+
+44
+00:03:17,980 --> 00:03:22,760
+أنواع الدهون type of lipid إيش أنواع الدهون الدهون
+
+45
+00:03:22,760 --> 00:03:28,360
+قُسمت بشكل عام إلى lipid with fatty acid و lipid
+
+46
+00:03:28,360 --> 00:03:34,060
+without fatty acid يعني الدهون بشكل عام قُسمت إلى
+
+47
+00:03:34,060 --> 00:03:39,620
+قسمين دهون تحتوي على fatty acid ودهون لا تحتوي على
+
+48
+00:03:39,620 --> 00:03:42,940
+fatty acid lipid with fatty acid و lipid without
+
+49
+00:03:42,940 --> 00:03:46,680
+fatty acidاللي with fatty acid تحتوي على fatty
+
+50
+00:03:46,680 --> 00:03:51,280
+acid من الامتل عليها ال waxes، fat and oil، الذبدة
+
+51
+00:03:51,280 --> 00:03:55,640
+ويئ الزيت، phospholipid، sphingolipid، lipid
+
+52
+00:03:55,640 --> 00:03:59,900
+without fatty acid زي الستيرون، هاي التقسيمة بشكل
+
+53
+00:03:59,900 --> 00:04:04,790
+عام وهالجيتها نبدأ نفرع منهالقيت احنا حاكي هنا a
+
+54
+00:04:04,790 --> 00:04:08,350
+classification هنا of lipid نبدأ نفعلها اللبد بشكل
+
+55
+00:04:08,350 --> 00:04:12,490
+عام تنقسم إلى lipid with fatty acid تحتوي على
+
+56
+00:04:12,490 --> 00:04:16,330
+fatty acid و lipid without fatty acid ده تحتوي على
+
+57
+00:04:16,330 --> 00:04:19,870
+fatty acid ناخد الجزء الأول اللي هي اللبد with
+
+58
+00:04:19,870 --> 00:04:24,770
+fatty acid اللي بتحتوي على fatty acid اللبد اللتي
+
+59
+00:04:24,770 --> 00:04:28,470
+تحتوي على fatty acid تنقسم إلى قسمين في منها
+
+60
+00:04:28,470 --> 00:04:33,780
+simple lipid و في منها conjugate في simpleوفي
+
+61
+00:04:33,780 --> 00:04:36,920
+conjugate هاي ال libid with fatty acid تنقسم إلى
+
+62
+00:04:36,920 --> 00:04:41,260
+simple و conjugate نحكي عن ال simple بيحكي إنه ال
+
+63
+00:04:41,260 --> 00:04:45,320
+simple libid تركيبتها الأساسية يبارح عن fatty acid
+
+64
+00:04:45,320 --> 00:04:50,340
+مع كحول إذا كان بس عبارة عن fatty acid و كحول
+
+65
+00:04:50,340 --> 00:04:53,840
+تركيب الأساسية إليه fatty acid و كحول بنسميه
+
+66
+00:04:53,840 --> 00:04:58,320
+simple libid بيحكي إنه الكحول هاد ممكن يكون
+
+67
+00:04:58,320 --> 00:05:03,780
+glycerol أو كحول نوع تاني غير ال glycerolإذا فاتي
+
+68
+00:05:03,780 --> 00:05:07,980
+أسد متصلة بكحول بسميها سم باللبن طب الكحول هاد إما
+
+69
+00:05:07,980 --> 00:05:11,680
+بيكون ممكن يكون نوعه إيش هيكون نوعه ممكن يكون
+
+70
+00:05:11,680 --> 00:05:16,240
+glycerol أو حاجة تانية كحول نوع تاني غير ال
+
+71
+00:05:16,240 --> 00:05:21,160
+glycerol كيف بيرتبط الفاتي أسد مع الكحول ب Easter
+
+72
+00:05:21,160 --> 00:05:24,560
+bond by Easter bond اللي هي الرابطة الدهنية هذا
+
+73
+00:05:24,560 --> 00:05:28,980
+اسم الرابطة الدهنية بيرتبط الفاتي أسد مع الكحول
+
+74
+00:05:28,980 --> 00:05:34,530
+برابطة دهنيةلو الكحول هات كان glycerol الناتج من
+
+75
+00:05:34,530 --> 00:05:38,970
+اللي بدهات بسميه اما oil او fat يعني اذا fatty
+
+76
+00:05:38,970 --> 00:05:44,110
+acid مرتبط معها glycerol بسميها oil هينتج عنها oil
+
+77
+00:05:44,110 --> 00:05:48,290
+او fat ال oil هو عبارة عن ال tri-glyceride rich in
+
+78
+00:05:48,290 --> 00:05:51,830
+unsaturated fatty acid و بيكون liquid at room
+
+79
+00:05:51,830 --> 00:05:55,210
+temperature الزيت ال oil هو عبارة عن ال tri
+
+80
+00:05:55,210 --> 00:05:58,630
+-glyceride tri معناها تلاتة يعني تلاتة fatty acid
+
+81
+00:05:58,900 --> 00:06:03,120
+مرتبطين مع glycerol مع الكحول try معناها تلاتة
+
+82
+00:06:03,120 --> 00:06:07,420
+fatty acid مع glycerol الكحول هذا ال oil بيكون
+
+83
+00:06:07,420 --> 00:06:11,460
+بيحكي ليهو unsaturated fatty acid يحتوي على
+
+84
+00:06:11,460 --> 00:06:15,880
+unsaturated fatty acid إيش يعني unsaturated يعني
+
+85
+00:06:15,880 --> 00:06:21,940
+أحماض دهنية غير مشبعة يعني تحتوي على رابطة ثنائية
+
+86
+00:06:21,940 --> 00:06:27,670
+فأكتر وال oil هات بيكون liquid قوامه بيكون سائلأما
+
+87
+00:06:27,670 --> 00:06:31,290
+الفات فهو برضه triglyceride تلاتة fatty acid مع
+
+88
+00:06:31,290 --> 00:06:35,750
+glycerol ولكن هو غني بال saturated fatty acid
+
+89
+00:06:35,750 --> 00:06:40,630
+أحماض دهنية مشبعة وبيكون على درجة حرارة الغرفة
+
+90
+00:06:40,630 --> 00:06:43,810
+بيكون عبارة عن solid أو semi solid بيكون صلب أو
+
+91
+00:06:43,810 --> 00:06:50,530
+شبه الصلب هذه الفات الزبدة طيب لو الكحول كانت
+
+92
+00:06:50,530 --> 00:06:53,610
+حاجتان غير ال glycerol كان عبارة عن fatty acid
+
+93
+00:06:53,610 --> 00:06:57,850
+أحمض دهنية مرتبط معها كحول غير ال glycerolبينتج
+
+94
+00:06:57,850 --> 00:07:02,690
+عندي حاجة اسمها waxes اللي هينتج عندي حاجة اسمها
+
+95
+00:07:02,690 --> 00:07:07,150
+waxes اذا اول نوع من احنا اللي بتقسمها بشكل عام
+
+96
+00:07:07,150 --> 00:07:10,010
+الى with fatty acid و without fatty acid ال with
+
+97
+00:07:10,010 --> 00:07:13,590
+fatty acid في منها simple و في منها conjugate ال
+
+98
+00:07:13,590 --> 00:07:17,010
+simple اللي هادا بيكون عبارة عن fatty acid مع كحول
+
+99
+00:07:17,010 --> 00:07:21,530
+اذا الكحول كان glycerol بيعطيني oil و fat اذا حاجة
+
+100
+00:07:21,530 --> 00:07:25,170
+تانية غير ال glycerol بيعطيني حاجة اسمها waxes
+
+101
+00:07:28,030 --> 00:07:30,930
+القسم التاني من اللبد with fatty acid هي ال
+
+102
+00:07:30,930 --> 00:07:36,930
+conjugate لبن conjugate لبن هي عبارة عن اللبد في
+
+103
+00:07:36,930 --> 00:07:41,350
+تركيبته بيكون بيحتوي على fatty acid مع كحول مع
+
+104
+00:07:41,350 --> 00:07:45,390
+additional group مع group كمان زيادة بيكون عبارة
+
+105
+00:07:45,390 --> 00:07:50,410
+عن تركيبته fatty acid أحمد دهنية مرتبطة مع الكحول
+
+106
+00:07:50,410 --> 00:07:54,570
+ومرتبط عليها كمان group زيادة additional group
+
+107
+00:07:55,420 --> 00:07:59,700
+fatty acid مع كحول مع additional group مع group
+
+108
+00:07:59,700 --> 00:08:07,500
+تاني لو الكحول لو مع additional group sorry مع
+
+109
+00:08:07,500 --> 00:08:10,780
+additional group مع group تاني الجد بناء على ال
+
+110
+00:08:10,780 --> 00:08:14,740
+group اللي بتتصل في هذا ال lipid بسميه lipid فإذا
+
+111
+00:08:14,740 --> 00:08:17,240
+ال group اللي اتصلت معاه كانت phosphate group
+
+112
+00:08:17,240 --> 00:08:21,400
+بسميه phospholipid phospholipid هو عبارة عن
+
+113
+00:08:21,400 --> 00:08:25,750
+conjugate lipid يحتوي على fatty acidمع كحول مع
+
+114
+00:08:25,750 --> 00:08:31,690
+phosphoric acid الـ glycolipid هو عبارة عن لبد
+
+115
+00:08:31,690 --> 00:08:36,010
+يحتوي .. كنجقة لبد يحتوي على fatty acid مع كحول مع
+
+116
+00:08:36,010 --> 00:08:39,230
+carbohydrate الـ glyco جاية من carbohydrate اللي
+
+117
+00:08:39,230 --> 00:08:44,930
+هي السكة الـ sulfolipid هو عبارة عن fatty acid مع
+
+118
+00:08:44,930 --> 00:08:50,490
+كحول مع sulfate groupفوسفو لبد فوسفات جروب بتكون
+
+119
+00:08:50,490 --> 00:08:54,930
+متصلة فيهم الجلايكو بتكون carbohydrate صلفو بيكون
+
+120
+00:08:54,930 --> 00:08:59,790
+صلفات جروب ليبوبروتين بيكون متصل معها البروتين
+
+121
+00:08:59,790 --> 00:09:06,090
+عبارة عن fatty acid مع كحول و البروتين متصلة فيها
+
+122
+00:09:06,090 --> 00:09:10,290
+لذا ال conjugate لبد هو عبارة عن fatty acid مع
+
+123
+00:09:10,290 --> 00:09:14,830
+كحول مع جروب كمان مضافة عليهاهذه ال group هي اللي
+
+124
+00:09:14,830 --> 00:09:17,630
+اعطته الاسم إذا هي phosphate group بسميها
+
+125
+00:09:17,630 --> 00:09:21,710
+phospholipin إذا carbohydrate بسميها glycolipin
+
+126
+00:09:21,710 --> 00:09:25,510
+إذا sulfate بسميها sulfolipin وإذا ال protein
+
+127
+00:09:25,510 --> 00:09:33,150
+بسميها lipoprotein هنا عندنا مخطط بيوضحلي تقسيمة
+
+128
+00:09:33,150 --> 00:09:36,330
+انه كيف بتقسم اللبن هو بشكل عام احنا هذه ال
+
+129
+00:09:36,330 --> 00:09:39,730
+structures مش مطلوبة مننا لكن بشكل عام نعرف ان
+
+130
+00:09:39,730 --> 00:09:44,380
+اللبن مقسم إلى with fatty acidwithout fatty acid
+
+131
+00:09:44,380 --> 00:09:47,640
+ال with fatty acid اللي اتفرعنا فيها في منها
+
+132
+00:09:47,640 --> 00:09:51,680
+simple و في منها conjugate ال simple هي عبارة عن
+
+133
+00:09:51,680 --> 00:09:55,700
+fatty acid مع كحول و الكحول هذا إما يكون glycerol
+
+134
+00:09:55,700 --> 00:09:59,100
+أو حاجة تانية غير ال glycerol إذا كان glycerol
+
+135
+00:09:59,100 --> 00:10:02,520
+بيعطيني fat و oilإذا حاجة تانية غير ال glycerol
+
+136
+00:10:02,520 --> 00:10:07,500
+بيعطيني waxes، waxes لبد أما الكنجوجات لبد هي
+
+137
+00:10:07,500 --> 00:10:11,720
+عبارة عن fatty acid مع كحول، مع other group، مع
+
+138
+00:10:11,720 --> 00:10:16,980
+group تانية متصلة فيهاو بناء على هاي ال group
+
+139
+00:10:16,980 --> 00:10:19,540
+نسميها phospholipid يعني phosphatic group
+
+140
+00:10:19,540 --> 00:10:23,240
+glycolipid يعني carbohydrate sulfolipid يعني
+
+141
+00:10:23,240 --> 00:10:28,340
+sulfatic group ليبوبروتين يعني متصل فيها بروتين ال
+
+142
+00:10:28,340 --> 00:10:31,660
+without fatty acid، lipid without fatty acid مش
+
+143
+00:10:31,660 --> 00:10:35,020
+متصل فيها fatty acid هي هذه الجزء اللي تحت هنا
+
+144
+00:10:35,020 --> 00:10:38,560
+الجزء
+
+145
+00:10:38,560 --> 00:10:43,200
+اللي تحت ماد لطويل و نازل لتحت مكتوب عندها steroid
+
+146
+00:10:45,230 --> 00:10:48,590
+مكتوب بـ steroids فاللبد الـ without fatty acid
+
+147
+00:10:48,590 --> 00:10:53,510
+نسميها steroids تحتوي على steroid nucleus تحتوي
+
+148
+00:10:53,510 --> 00:10:57,910
+على steroid nucleus من الأمثلة على اللبد اللتي لا
+
+149
+00:10:57,910 --> 00:11:02,170
+تحتوي fatty acid تحتوي على steroid nucleus زي
+
+150
+00:11:02,170 --> 00:11:08,750
+كلسترول و bile salt و steroid hormone كلسترول و
+
+151
+00:11:08,750 --> 00:11:13,010
+bile salt و steroid hormone هدولة لبد without
+
+152
+00:11:13,010 --> 00:11:18,390
+fatty acidطب اليوم الشغل العملي هو عبارة عن
+
+153
+00:11:18,390 --> 00:11:27,330
+qualitative test for لبن لنعمل فحوصات جودة
+
+154
+00:11:27,330 --> 00:11:32,440
+و نوعية للكشف عن اللبن فحوصات qualitativeيعني جودة
+
+155
+00:11:32,440 --> 00:11:36,780
+و نوعية زي فحوصات الكشف عن الأحمد الأمنية فهنا
+
+156
+00:11:36,780 --> 00:11:40,380
+مجموعة فحوصات ممكن يتغير اللون أو مايتغيرش اللون
+
+157
+00:11:40,380 --> 00:11:46,600
+ونشوف فحوصات للكشف عن ال lipids زي مين أول فحص
+
+158
+00:11:46,600 --> 00:11:49,660
+هنتعرف عليه هو solubility in polar and nonpolar
+
+159
+00:11:49,660 --> 00:11:54,600
+solvent فهنا فحص اسمه emulsification test اللي هو
+
+160
+00:11:54,600 --> 00:11:58,540
+الاستحلاب فهنا فحص ال oxidation of unsaturated
+
+161
+00:11:58,540 --> 00:12:03,480
+fatty acid أكسداللـ unsaturated fatty acid ويعني
+
+162
+00:12:03,480 --> 00:12:07,140
+فحص qualitative analysis of detection in natural
+
+163
+00:12:07,140 --> 00:12:12,400
+product اللي هو فحص اسمه سالكا ويسكي reaction هنجي
+
+164
+00:12:12,400 --> 00:12:17,640
+نتعرف على كل فحص على حدة أول فحص هو solubility in
+
+165
+00:12:17,640 --> 00:12:22,200
+polar and nonpolar solvent solubility in polar and
+
+166
+00:12:22,200 --> 00:12:27,570
+nonpolar يعني بدي أجيب اللي بدهونو بدي أدوبه في
+
+167
+00:12:27,570 --> 00:12:32,650
+مواد .. بدي أجيبه و أحطه في مواد عضوية و غير عضوية
+
+168
+00:12:32,650 --> 00:12:36,250
+و أشوف وين يدوب يعني انت جيبله مواد polar و non
+
+169
+00:12:36,250 --> 00:12:39,850
+-polar حاجات اللي بتحكي ان هو non-polar بيدوب في
+
+170
+00:12:39,850 --> 00:12:43,310
+ال non-polar فإذا جبتله non-polar بيدوب فيه إذا
+
+171
+00:12:43,310 --> 00:12:47,370
+جبتله polar مش هيدوب فيه احنا في هذا الفحص بدنا
+
+172
+00:12:47,370 --> 00:12:51,990
+نجيب مي نجيب chloroform نجيب ether نجيب إيثانوانو
+
+173
+00:12:51,990 --> 00:12:55,630
+ندوب اللبد فيهم الزيت مثلا نجيب زيت ندوبه في الماء
+
+174
+00:12:55,630 --> 00:12:59,810
+نشوف انه بدوب ولا بدوبش بعدين بدنا نجيب الزيت و
+
+175
+00:12:59,810 --> 00:13:05,270
+ندوبه في ايه نشوف هل هيدوب ولا مش هيدوب بعدين نجيب
+
+176
+00:13:05,270 --> 00:13:09,530
+كلوروفارب و ندوبه فيه نشوف هل اللبد هيدوب ولا مش
+
+177
+00:13:09,530 --> 00:13:14,790
+هيدوب هنلاحظ ان اللبد بدوب فيهم كله تم هذا ال
+
+178
+00:13:14,790 --> 00:13:19,010
+distill water لأنه عبارة عن boiler و اللبد non
+
+179
+00:13:19,010 --> 00:13:25,110
+boiler فبيدوبش فيهفمبدأ عمل الفحص الـ principle هو
+
+180
+00:13:25,110 --> 00:13:29,650
+على أي مبدأ بناء على إيش هيدوب اللبط في الماء،
+
+181
+00:13:29,650 --> 00:13:33,150
+هيدوب في المركبة العضوية ومش هيدوب في الماء، بناء
+
+182
+00:13:33,150 --> 00:13:37,510
+على أي مبدأ، حكينا بناء على مبدأ like, dissolve,
+
+183
+00:13:37,870 --> 00:13:42,350
+likeلذن ال principle هو like dissolve like ال
+
+184
+00:13:42,350 --> 00:13:45,290
+objective من هذا الفحص أنه أنا بدي أكشف عن
+
+185
+00:13:45,290 --> 00:13:48,650
+solubility of lipids in water and organic solvent
+
+186
+00:13:48,650 --> 00:13:53,330
+اللي هي مادة ذوبانية الدهون في المي و في المديبات
+
+187
+00:13:53,330 --> 00:13:57,950
+العضويةالصليبوليتي بتعتمد على ال polarity
+
+188
+00:13:57,950 --> 00:14:01,770
+الدوبانية بتعتمد على القطمية طيب ايش ال reagent
+
+189
+00:14:01,770 --> 00:14:05,570
+اللى بدنا نستخدمه في الفحص هاد؟ بدنا نستخدم دهون،
+
+190
+00:14:05,570 --> 00:14:08,450
+بدنا نستخدم زيت الزتون و الفات اللى هى ال butter
+
+191
+00:14:08,450 --> 00:14:12,310
+أو الفات اللى هى الزبدة المواد اللى بدنا ندوب فيها
+
+192
+00:14:12,310 --> 00:14:17,470
+ال solvent هي بدنا نستخدم إيثانول و chloroform و
+
+193
+00:14:17,470 --> 00:14:23,470
+ether و waterكيف بدي أشتغل الفحص؟ بدي أجيب أربع
+
+194
+00:14:23,470 --> 00:14:27,350
+تيوبات واحدة تشتغل فيها ديستل واتر واحدة إيثر
+
+195
+00:14:27,350 --> 00:14:31,990
+واحدة كلوروفور واحدة إيثانور تمام؟ بدي أجيب لتيوب
+
+196
+00:14:31,990 --> 00:14:35,670
+الأول تاعة الديستل واتر بدي أحط فيها drop oil drop
+
+197
+00:14:35,670 --> 00:14:40,590
+من الزيت على واحد مل من الديستل واتر بدي أجيب
+
+198
+00:14:40,590 --> 00:14:45,000
+لتيوب الأولأحط فيها drop من ال oil نقطة من الزيت
+
+199
+00:14:45,000 --> 00:14:48,180
+على واحد من ال distill water و أعمل mix أشوف هل
+
+200
+00:14:48,180 --> 00:14:52,660
+هيكون دائب ولا غير دائب soluble او insoluble ال
+
+201
+00:14:52,660 --> 00:14:56,320
+cube التانية بدي أحط عليها drop من ال oil على واحد
+
+202
+00:14:56,320 --> 00:15:01,060
+من ال ether بدي أحط منها drop من الزيت على واحد من
+
+203
+00:15:01,060 --> 00:15:05,330
+ال ether و أشوف بيدوب ولا بيدوبشهجيب ال tube
+
+204
+00:15:05,330 --> 00:15:09,290
+التالت فيها chlorofar حط واحد drop من الزيت على
+
+205
+00:15:09,290 --> 00:15:13,510
+واحد من ال chlorofar هشوف بيدوب ولا لأ، ال tube
+
+206
+00:15:13,510 --> 00:15:16,850
+الرابع ده حط فيها drop من ال oil على واحد من ال
+
+207
+00:15:16,850 --> 00:15:21,960
+ethanol، هشوف بيدوب ولا بيدوبشنفس الخطوات بدي أجيب
+
+208
+00:15:21,960 --> 00:15:25,580
+أربعة يوبات تانية و أعمل فيها نفس الخطوات اللي هي
+
+209
+00:15:25,580 --> 00:15:28,480
+واحدة distil water واحدة ether واحدة chloroform
+
+210
+00:15:28,480 --> 00:15:32,020
+واحدة ethanol بس بدل الزيت بدي أحط butter بدي أحط
+
+211
+00:15:32,020 --> 00:15:35,240
+زبدة زبدة مع distil water شوف بيدوب ولا بيدوبش
+
+212
+00:15:35,240 --> 00:15:39,200
+ether مع زبدة شوف بيدوب ولا بيدوبش chloroform مع
+
+213
+00:15:39,200 --> 00:15:42,880
+زبدة شوف بيدوب ولا بيدوبش ethanol مع زبدة هل بيدوب
+
+214
+00:15:42,880 --> 00:15:47,380
+ولا مابيدوبش ده شوف هدول بيدوبوا ولا مابيدوبوامش
+
+215
+00:15:47,380 --> 00:15:52,060
+بنتوقع؟ بنتوقع إنه يدوب في كل ما عدا الـ distilled
+
+216
+00:15:52,060 --> 00:15:58,640
+water مش هيدوب في الـ distilled water هدر بالنسبة
+
+217
+00:15:58,640 --> 00:16:02,880
+للفحص الأول فحص التاني هو اسمه ال emulsification
+
+218
+00:16:02,880 --> 00:16:08,440
+emulsification معناه استحلامemulsification معناها
+
+219
+00:16:08,440 --> 00:16:14,780
+استحلاب إيش يعني استحلاب؟ يعني جعل مادتين ما
+
+220
+00:16:14,780 --> 00:16:18,540
+بيدوبوا ببعض طبيعي اللي هي دوبوا ببعض بدي أخليهم
+
+221
+00:16:18,540 --> 00:16:24,480
+بيدوبوا ببعض بمساعدة emulsifying agent بمساعدة
+
+222
+00:16:24,480 --> 00:16:29,270
+عامل مستحلبإذا ال emulsification أنه أنا بدي أخلي
+
+223
+00:16:29,270 --> 00:16:33,550
+مادتين هم أصلا ما بيدوبوا ببعض بدي أخليهم يدوبوا
+
+224
+00:16:33,550 --> 00:16:39,210
+بواسطة عامل مستحلب بمساعدة عامل مستحلب زي مين
+
+225
+00:16:39,210 --> 00:16:42,650
+مادتين بدوبوش ببعض مثلا الزيت مع الميه بدوبوش ببعض
+
+226
+00:16:42,650 --> 00:16:47,490
+أنا بدي أجيب عامل مستحلب بيساعد على أنه الزيت يدوب
+
+227
+00:16:47,490 --> 00:16:52,150
+في الميهزي مثلا هلجيت لو عندنا مجلة المجلة هي هاد
+
+228
+00:16:52,150 --> 00:16:56,150
+فيه زيت و أنت تجيتي تجليه، تخسليه، لو حطيت الميه و
+
+229
+00:16:56,150 --> 00:17:00,890
+خلاص، هالخلاص هينظف و انه هيروح الزيت منه؟ لأ،
+
+230
+00:17:00,890 --> 00:17:04,710
+بتحس انه الزيت يتكوم زي قطرات في هذا المجلة، انت
+
+231
+00:17:04,710 --> 00:17:08,010
+شو بتعمل عشان تشيلي هذا الزيت و تنظفيه خالص؟ بتحطي
+
+232
+00:17:08,010 --> 00:17:12,830
+صابونالصابون بيساعد على إنه يدوب الزيت في المي و
+
+233
+00:17:12,830 --> 00:17:16,470
+يشيل الزيت من المجلة كان جابل الزيت مش راضي يروح
+
+234
+00:17:16,470 --> 00:17:19,850
+بالمي لما حطيتي صابونة الصابونة ساعدت فهنا
+
+235
+00:17:19,850 --> 00:17:24,830
+الصابونة بسميها كإنها emulsifying agent عامل
+
+236
+00:17:24,830 --> 00:17:31,580
+مستحلب ساعد على دوبان الزيت في المي و إنه يختفيمش
+
+237
+00:17:31,580 --> 00:17:34,480
+بيحكيلي أن ال emulsion هو mixture of two or more
+
+238
+00:17:34,480 --> 00:17:38,100
+liquids are normally immiscible، ال emulsion هو
+
+239
+00:17:38,100 --> 00:17:42,520
+عبارة عن خليت من مادتين أو أكتر طبيعيين ما بيدوبوا
+
+240
+00:17:42,520 --> 00:17:47,780
+بالمي زي زيت مع مي و بيدوبوش في بعض طبيعيين،
+
+241
+00:17:47,780 --> 00:17:52,840
+هبسميه emulsionهلجت ال bile salt هيش هي ال bile
+
+242
+00:17:52,840 --> 00:17:56,760
+salt؟ ال bile salt هي ملح العصارة السفراوية بتنتج
+
+243
+00:17:56,760 --> 00:18:01,060
+من الكوليسترول تصنع من الكوليسترول وتخزن في الجالب
+
+244
+00:18:01,060 --> 00:18:08,260
+لدر في المثانا في المرارة تخزن في المرارة لما ان
+
+245
+00:18:08,260 --> 00:18:11,500
+احنا الجسم الحين في جسم الإنسان أغلب مكوناته ماي
+
+246
+00:18:11,500 --> 00:18:14,680
+طب اللي الإنسان لما ياكل واجب الدهون كيف هيبدأ
+
+247
+00:18:14,680 --> 00:18:18,570
+تدوب و تمشي في جسم الإنسان اللي أغلبه ماي؟بحكي إنه
+
+248
+00:18:18,570 --> 00:18:22,290
+إحنا في جسم الإنسان when you eat فات لما تاكلي
+
+249
+00:18:22,290 --> 00:18:26,370
+وجبة دهون، في عندنا ال bile salt بتشتغل في الجسم
+
+250
+00:18:26,370 --> 00:18:30,670
+كصابونة، زي صابونة كأنها، بتدوب ال bile salt و
+
+251
+00:18:30,670 --> 00:18:34,910
+بتخليها ماشية في الجسم بسهولة بين الماءكيف؟ ال
+
+252
+00:18:34,910 --> 00:18:38,590
+bile salt هاي .. هاي الأصفر اللي تحت أصفر ويلها
+
+253
+00:18:38,590 --> 00:18:42,850
+طرف أخضر مكتوب عليها أنها bile salt إيش هاي الطرف
+
+254
+00:18:42,850 --> 00:18:46,810
+الأخضر؟ هاي bile salt إلها جزءين جزء بكون .. بكون
+
+255
+00:18:46,810 --> 00:18:49,550
+منها hydrophilic اللي هو الأخضر الجزء الأخضر
+
+256
+00:18:49,550 --> 00:18:52,570
+hydrophilic والجزء الأصفر بيكون عبارة عن
+
+257
+00:18:52,570 --> 00:18:57,410
+hydrophobic جزء بيكون منها محب للماء وجزء بيكون
+
+258
+00:18:57,410 --> 00:19:01,390
+كاره للماءكيف تيجي تعمل هاي ال bile salt؟ بتيجي
+
+259
+00:19:01,390 --> 00:19:04,310
+على وجبة الدهون اللي عندها، هاي الزرقة الكبيرة
+
+260
+00:19:04,310 --> 00:19:09,710
+بتحوت عليها من جميع الاتجاهات بحيث أنه جزء الكاره
+
+261
+00:19:09,710 --> 00:19:14,770
+للماء يكون للداخل منها والجزء اللي بيحب الماء يكون
+
+262
+00:19:14,770 --> 00:19:19,790
+للخارج فالحين هي كأنها كورة ماشية في الجسم من جوا
+
+263
+00:19:19,790 --> 00:19:24,450
+بتدوبش في الماء لكن من برا محبة للماء فمهما تمشي
+
+264
+00:19:24,450 --> 00:19:28,750
+في الجسم هتبين كأنها دائبة في الماءفالدهون كيف
+
+265
+00:19:28,750 --> 00:19:32,850
+بتمشي في الجسم عن طريق ال bile salt اللي بتعمل
+
+266
+00:19:32,850 --> 00:19:38,310
+كسبونة بتحوت عليها من الداخل بجزء مكاره للماء
+
+267
+00:19:38,310 --> 00:19:46,910
+هيدروفوبك ومن الخارج بجزء هيدروفليك محب للماء طيب
+
+268
+00:19:46,910 --> 00:19:50,130
+هلجيت ال emulsification اللي هو الاستحلاب فيه منه
+
+269
+00:19:50,130 --> 00:19:53,250
+نوعين ننزل تحت على آخر ال slide في emulsification
+
+270
+00:19:53,250 --> 00:19:57,440
+type أنواع الاستحلابفي منه permanent وفي منه
+
+271
+00:19:57,440 --> 00:20:01,520
+temporary permanent يعني بيكون استحلاق بشكل دائم
+
+272
+00:20:01,520 --> 00:20:06,040
+يعني الزيت مع مي ودائبي بشكل دائم مهما أسيب التيوب
+
+273
+00:20:06,040 --> 00:20:09,800
+أرجها و أرجع أسيبها بخلق بلاجيهم دايما منتشرين
+
+274
+00:20:09,800 --> 00:20:14,680
+الزيت منتشر في المي temporary انه الزيت مع مي
+
+275
+00:20:14,680 --> 00:20:18,940
+بتعملي mix بدوبوا ببعض مجرد ما تسيب التيوب بيرجع
+
+276
+00:20:18,940 --> 00:20:23,070
+يفصل طبقتين الزيت لحال و المي لحالفال permanent
+
+277
+00:20:23,070 --> 00:20:26,990
+بيكون viscous and stable they don't separate بيكون
+
+278
+00:20:26,990 --> 00:20:30,630
+لزج قوامه لزج لأنه مادتين غير دائبين كإن إساره
+
+279
+00:20:30,630 --> 00:20:35,470
+دائبين بيكون stable مستقر ومهما تسيبيه don't
+
+280
+00:20:35,470 --> 00:20:38,590
+separate ما بيفصلوش برجعوش يفصلوا الطبقتين وإنما
+
+281
+00:20:38,590 --> 00:20:42,790
+بيضلهم دائبين بعض permanent بشكل ده أما ال
+
+282
+00:20:42,790 --> 00:20:46,540
+temporaryجاي from shaking بيحكي لإنت بتمسكي التيوب
+
+283
+00:20:46,540 --> 00:20:50,080
+و بتحطي زيت على الماء اعملي mix رشجي لتيوب بإيدك
+
+284
+00:20:50,080 --> 00:20:53,780
+بتحس إنه الزيت انتشر في الماء بمجرد ما تسيبي
+
+285
+00:20:53,780 --> 00:20:58,840
+التيوب بيرجع يفصل لطبقتين الزيت لحال و المي لحال
+
+286
+00:20:58,840 --> 00:21:03,600
+هذا بيكون temporary بشكل معقدهو رجعنا على ال slide
+
+287
+00:21:03,600 --> 00:21:07,500
+من أولها من فوق بيحكيلي هنا في عندنا أربع أربع
+
+288
+00:21:07,500 --> 00:21:13,180
+شكال على الجانب A وB وC وD في A موجودة عندى حاطين
+
+289
+00:21:13,180 --> 00:21:17,460
+خليط من مادتين سائلين مابدوبوش ببعض زى الزيت والمي
+
+290
+00:21:17,460 --> 00:21:20,820
+لو اعتبرنا انه الجزء اللى فوق هو الزيت والجزء اللى
+
+291
+00:21:20,820 --> 00:21:24,640
+تحت مي ففي أش في صورة A شايفين two immiscible
+
+292
+00:21:24,640 --> 00:21:29,040
+liquids not yet emulsified انه خليطين من خليط من
+
+293
+00:21:29,040 --> 00:21:33,550
+مادتين أصلا بدوبوش بعض immiscible لا يختلطواولا
+
+294
+00:21:33,550 --> 00:21:36,250
+حطينا لهم emulsifying agent عشان نعملهم
+
+295
+00:21:36,250 --> 00:21:40,950
+emulsification ماحطيناهاش عامل مستحيل بيساعد على
+
+296
+00:21:40,950 --> 00:21:45,030
+أنهم يدوبوا أبعاد بحكي اللي في بيه لو طلعت عليها
+
+297
+00:21:45,030 --> 00:21:48,250
+بتحس أنه الجزء اللي كان فوق انتشر في اللي تحت يعني
+
+298
+00:21:48,250 --> 00:21:52,630
+الزيت انتشر في الماء قطارات الزيت انتشرت في الماء
+
+299
+00:21:52,630 --> 00:21:56,430
+بحكي لان emulsion of phase two disappears in phase
+
+300
+00:21:56,430 --> 00:22:00,130
+one أنه انتشرت الجزء اللي فوق في الجزء اللي تحت
+
+301
+00:22:00,130 --> 00:22:05,490
+مبين كأنهم ذائبينفي C بيحكيلي unstable emulsion
+
+302
+00:22:05,490 --> 00:22:09,450
+separate كان هذا ال emulsion الاستحلاب كان مؤقت
+
+303
+00:22:09,450 --> 00:22:13,610
+كان temporary جاي من عملية shaking فمجرد ما سيبنا
+
+304
+00:22:13,610 --> 00:22:17,790
+التيوب في C ع طول فصل رجع فصل طبقتين طبقة اللي فوق
+
+305
+00:22:17,790 --> 00:22:22,590
+لحال و اللي تحت لحال الزيت فوق و المي تحت في D
+
+306
+00:22:22,590 --> 00:22:27,450
+بيحكيلي استخدمنا surfactant surfactant هو نفسه
+
+307
+00:22:27,450 --> 00:22:33,560
+emulsifying agent هو عامل مستحلباستخدم في C في D
+
+308
+00:22:33,560 --> 00:22:37,320
+استخدم surfactant عامل مستحلب Emulsifying agent
+
+309
+00:22:37,320 --> 00:22:42,280
+إيش عمل هذا العامل عمل على حشر نفسه position
+
+310
+00:22:42,280 --> 00:22:46,200
+itself on interface between face one و face two
+
+311
+00:22:46,200 --> 00:22:52,170
+حشر نفسه بين الطبقة اللي فوق و الطبقة اللي تحتيعني
+
+312
+00:22:52,170 --> 00:22:55,130
+كأنه زي الصابونة زي الـ Bile Salt اللي تاوم حكينا
+
+313
+00:22:55,130 --> 00:22:58,990
+عنها أنها بتربط بجزء من الداخل Hydrophobic ومن
+
+314
+00:22:58,990 --> 00:23:03,190
+الخارج Hydrophilic بتربط في الزيت بحيث أنه يمشي
+
+315
+00:23:03,190 --> 00:23:07,010
+بالمي كأنه دائب ففي دي أنا استخدمت Emulsifying
+
+316
+00:23:07,010 --> 00:23:10,870
+agent حشر نفسه في الزيت بين الزيت والمي وخلّى
+
+317
+00:23:10,870 --> 00:23:15,560
+الزيت كأنه دائب في الميو هذا كان بشكل permanent
+
+318
+00:23:15,560 --> 00:23:20,900
+بشكل دائم فمهما سبتيها مش هيفصل عن بعض بسبب وجود
+
+319
+00:23:20,900 --> 00:23:22,920
+ال emulsifying agent
+
+320
+00:23:25,960 --> 00:23:30,420
+كيف نعمل فحصنا لليوم؟ لنستخدم emulsifying agent ده
+
+321
+00:23:30,420 --> 00:23:35,020
+أجيب زيت و أحط عليه مي لو رجيتهم هم هيدوبوا و
+
+322
+00:23:35,020 --> 00:23:38,480
+بعدين هترجع تفصل لطبقتين تاني بس عشان يرجع تلت يوم
+
+323
+00:23:38,480 --> 00:23:42,120
+بدي أستخدم في هذا الفحص emulsifying agent أحط زيت
+
+324
+00:23:42,120 --> 00:23:46,520
+على مي و أستخدم عامل مستحلب يساعد على دوابنيتهم
+
+325
+00:23:46,520 --> 00:23:50,800
+ببعض إيش العامل المستحلب اللي بدي أستخدمه هو نصف
+
+326
+00:23:50,800 --> 00:23:56,640
+الميةNA2CO3 Sodium Bicarbonate هذا إيش وظيفته في
+
+327
+00:23:56,640 --> 00:24:01,800
+هذا الفحص وظيفته Emulsifying Agent عامل مستحل
+
+328
+00:24:01,800 --> 00:24:06,900
+وبدنا نشتغل الفحص على زيت زتون طبيعي Natural Olive
+
+329
+00:24:06,900 --> 00:24:11,740
+Oil وعلى Rancid Olive Oil على زيت زتون طبيعي وزيت
+
+330
+00:24:11,740 --> 00:24:17,180
+الزتون المتزنخnatural olive oil زيت الزيتون
+
+331
+00:24:17,180 --> 00:24:20,640
+الطبيعي و نستخدم rancid olive oil اللي هو زيت
+
+332
+00:24:20,640 --> 00:24:24,420
+الزيتون المتزنخ ايش الفرق بين الاتنين؟ بيحكي لل
+
+333
+00:24:24,420 --> 00:24:27,040
+natural olive oil اللي هو زيت الزيتون الطبيعي
+
+334
+00:24:27,040 --> 00:24:31,200
+بيكون جديد، بيكون لسه مش ساير عليه عمليات أكسدة،
+
+335
+00:24:31,200 --> 00:24:34,380
+بيكون سايرله بس partial oxidation عمليات أكسدة
+
+336
+00:24:34,380 --> 00:24:39,770
+بسيطة جزئيةلما تأكسدى انتهى أي جزاء تأكسدى هذا
+
+337
+00:24:39,770 --> 00:24:44,810
+الزيت بينتج عنه تكسير للفاتي أسد بتتكسر بتتفكك عن
+
+338
+00:24:44,810 --> 00:24:48,510
+بعضها الفاتي أسد وبتتحرر فبيصير عندى فيه free
+
+339
+00:24:48,510 --> 00:24:52,330
+fatty acid في ال natural olive oil بيكون partially
+
+340
+00:24:52,330 --> 00:24:57,690
+ال oxidation عمليات أكسدة جزئية بسيطة فكمية الفاتي
+
+341
+00:24:57,690 --> 00:25:02,370
+أسد الحرة بتكون قليلة يحتوي على few of free fatty
+
+342
+00:25:02,370 --> 00:25:06,280
+acidلكن في الـ Rancid Olive Oil في زيت الزتون
+
+343
+00:25:06,280 --> 00:25:10,060
+المتزنخ اللي هو مجلة فيه أكتر من مرة عاملينه
+
+344
+00:25:10,060 --> 00:25:14,160
+مستخدمينه أكتر من مرة بيكون صاير عليه Complete
+
+345
+00:25:14,160 --> 00:25:19,720
+Oxidation عمليات أكسدة كاملة بتأدي إلى تحرير جميع
+
+346
+00:25:19,720 --> 00:25:23,360
+الفاتي أسد فبيكون عندنا many of free fatty acid
+
+347
+00:25:23,360 --> 00:25:28,980
+الفاتي أسد بتكون الـ free الحرة بتكون بشكل أكبرإيش
+
+348
+00:25:28,980 --> 00:25:33,120
+هي Rancidity؟ Rancidity معناها التزنخ Rancidity
+
+349
+00:25:33,120 --> 00:25:38,840
+معناها التزنخ هي ظهور رائحة كريهة أو غير مرغوب
+
+350
+00:25:38,840 --> 00:25:43,800
+فيها على الزيت والزبدة وهي الرائحة غالبا بتكون
+
+351
+00:25:43,800 --> 00:25:49,680
+مصحوبة بتغير في القوام والشكل لذن ال Rancidity هو
+
+352
+00:25:49,680 --> 00:25:55,860
+التزنخ وظهور رائحة غير مرغوب فيهامصحوبة بتغيير في
+
+353
+00:25:55,860 --> 00:25:59,740
+القوام والشكل على الزيت والزبدة
+
+354
+00:26:02,130 --> 00:26:05,870
+مش خطواتنا كيف نشتغل الفحص تبع ال emulsification
+
+355
+00:26:05,870 --> 00:26:09,650
+إذا انفهمنا أن ال objective من الفحص أن أنا بدي
+
+356
+00:26:09,650 --> 00:26:14,150
+أعمل emulsified oil and water in each other by
+
+357
+00:26:14,150 --> 00:26:18,410
+emulsifying agent بدي أساعد ال oil والwater، المي
+
+358
+00:26:18,410 --> 00:26:22,530
+والزيت ان هم يصيروا emulsified بعض بمساعدة من؟
+
+359
+00:26:22,530 --> 00:26:27,150
+emulsifying agentاللي هو عبارة عن الـ Sodium
+
+360
+00:26:27,150 --> 00:26:30,870
+Bicarbonate فلو كتبتوا .. كتبنا لبرنزميل عن النظام
+
+361
+00:26:30,870 --> 00:26:35,730
+معادلة ده هذا الفحص أحكي fat أو oil اللي هو الزيت
+
+362
+00:26:35,730 --> 00:26:39,690
+مع water بدي أضيف عليه ميه في وجود emulsifying
+
+363
+00:26:39,690 --> 00:26:42,950
+agent اللي هو Sodium Bicarbonate إيش بدي يصير
+
+364
+00:26:42,950 --> 00:26:46,490
+عندي؟ بدي يصير عندي emulsification بدي يصير عندي
+
+365
+00:26:46,490 --> 00:26:50,740
+emulsification الناتجخطوات العمل بدي أجيب تالتة
+
+366
+00:26:50,740 --> 00:26:54,320
+تيوب بقى تيوب الأول بدي أشتغل عليه بدي أحط فيه
+
+367
+00:26:54,320 --> 00:26:58,020
+drop من ال oil زيت زتون مع 2 ml distilled water و
+
+368
+00:26:58,020 --> 00:27:02,740
+أعمل mix بس حطيت زيت مع مي و عملتي mix إيش بدي
+
+369
+00:27:02,740 --> 00:27:06,580
+يصير؟ بتلاقي الزيت قطرات الزيت بتنتشر في المي و
+
+370
+00:27:06,580 --> 00:27:10,620
+مجرد ما تسيبي التيوب بيرجع يفصل لطبقتين يعني هو
+
+371
+00:27:10,620 --> 00:27:16,110
+insoluble لإن زيت مع مي بدوبوشالتيوب التاني بدي
+
+372
+00:27:16,110 --> 00:27:19,990
+تحطي عليها خمسة مل من الـ Distilled water على
+
+373
+00:27:19,990 --> 00:27:23,850
+اتنين لتلاتة drop من ال Sodium Bicarbonate على
+
+374
+00:27:23,850 --> 00:27:27,910
+drop من ال Oil تحط خمسة مل Distilled water اتنين
+
+375
+00:27:27,910 --> 00:27:30,750
+لتلاتة drop من ال Sodium Bicarbonate اللي هو ال
+
+376
+00:27:30,750 --> 00:27:34,710
+emulsifying agent على drop من الزيت لجيت حطينا زيت
+
+377
+00:27:34,710 --> 00:27:38,070
+مع مي و حطينا عليهم emulsifying agent المفروض انه
+
+378
+00:27:38,070 --> 00:27:43,170
+يساعد على عمل emulsification و يبينوا كأنهم دائبين
+
+379
+00:27:43,170 --> 00:27:48,270
+في بعضلكن بيحكيلي في هذه اليوتيوب لأن انت حاطيتي
+
+380
+00:27:48,270 --> 00:27:52,270
+oil natural olive oil زيت الزيتون الطبيعي الذي
+
+381
+00:27:52,270 --> 00:27:57,090
+يحتوي على كمية قليلة من ال free fatty acid بيقدرش
+
+382
+00:27:57,090 --> 00:28:01,050
+ييجي ال sodium bicarbonate يحشر بينهم ويرتبط معها
+
+383
+00:28:01,050 --> 00:28:04,350
+مافيش free fatty acid حرة انه يقدر يرتبط معها
+
+384
+00:28:04,350 --> 00:28:08,250
+ويعمل emulsificationفبيكون هنا بيعمل بشكل بس
+
+385
+00:28:08,250 --> 00:28:12,170
+temporary بتحس إنه الزيت مع المي إنهم دابوا ببعض
+
+386
+00:28:12,170 --> 00:28:16,430
+لكن سرعان ما يفصلوا عن بعض يفصل لطبقتين فبيكون
+
+387
+00:28:16,430 --> 00:28:19,410
+عبارة عن temporary emulsification صار بشكل
+
+388
+00:28:19,410 --> 00:28:23,590
+temporary ليش؟ لأن ال natural olive oil يحتوي على
+
+389
+00:28:23,590 --> 00:28:27,610
+few free fatty acids كمية الحماض الدهنية ال free
+
+390
+00:28:27,610 --> 00:28:32,580
+بتكون قليلةالتيوب التالت بدي أضع عليها 5 مل من
+
+391
+00:28:32,580 --> 00:28:35,960
+الـDistill water على 2 إلى 3 مل من الـ Sodium
+
+392
+00:28:35,960 --> 00:28:39,520
+bicarbonate اللي هو الـ Emulsifying agent بدي أضع
+
+393
+00:28:39,520 --> 00:28:44,040
+drop من الـ Rancid oil من الزيت الزتون المتزنخ
+
+394
+00:28:44,040 --> 00:28:48,580
+متزنخ يعني يحتوي على كمية كبيرة من الـ Free fatty
+
+395
+00:28:48,580 --> 00:28:53,700
+acid حطيتي منها هذا بيقدر ييجي الـ Emulsifying
+
+396
+00:28:53,700 --> 00:28:57,060
+agent اللي هي Sodium carbonate أنه يرتبط مع الـ
+
+397
+00:28:57,060 --> 00:29:01,040
+Free fatty acid الحرة هذهو يعمل emulsification
+
+398
+00:29:01,040 --> 00:29:06,680
+بشكل permanent بيكون بشكل permanent بشكل دائم،
+
+399
+00:29:06,680 --> 00:29:14,160
+دائمين بعض بشكل دائم الفحص التالت هو oxidation of
+
+400
+00:29:14,160 --> 00:29:18,500
+unsaturated fatty acid بتدعم الأكسدة لل
+
+401
+00:29:18,500 --> 00:29:22,780
+unsaturated fatty acidبعدين في اكسدة في تفاعلات
+
+402
+00:29:22,780 --> 00:29:26,220
+اكسدة واختزال اذا انا محتاجة ل oxidizing agent
+
+403
+00:29:26,220 --> 00:29:31,180
+لعامل مؤكسد عشان ي oxidize ال unsaturated fatty
+
+404
+00:29:31,180 --> 00:29:35,080
+acid ايش يعني unsaturated fatty acid حكينا
+
+405
+00:29:35,080 --> 00:29:39,000
+unsaturated معناه انه بيحتوي على double bond على
+
+406
+00:29:39,000 --> 00:29:43,480
+one or more double bond عنده double bond واحدة او
+
+407
+00:29:43,480 --> 00:29:47,860
+اكترفي عندنا نوعين من ال Acid هدول اللي بنستخدمهم
+
+408
+00:29:47,860 --> 00:29:51,040
+في عندنا Olic Acid هو عبارة عن Unsaturated Fatty
+
+409
+00:29:51,040 --> 00:29:55,620
+Acid وفي Steric Acid هو Saturated Fatty Acid
+
+410
+00:29:55,620 --> 00:29:59,260
+بنستخدم هدول اتنين في هذا الفحص اليوم مين اللي
+
+411
+00:29:59,260 --> 00:30:02,940
+هيعطيني positive بس ال Unsaturated Fatty Acid اللي
+
+412
+00:30:02,940 --> 00:30:06,800
+هو ال Olic Acid ال Olic Acid هيتغير لونه ويعطيني
+
+413
+00:30:06,800 --> 00:30:10,720
+positive لأنه بيصيرله أكس دهأما الـ stearic acid
+
+414
+00:30:10,720 --> 00:30:14,940
+بيصيرش عليه أكثر ده لأنه عبارة عن saturated fatty
+
+415
+00:30:14,940 --> 00:30:20,230
+acidكيف بيدعم ال Oxidation؟ بدي Oxidizing agent
+
+416
+00:30:20,230 --> 00:30:24,230
+اللي هو عامل مؤكسد العامل المؤكسد اللي بنستخدمه في
+
+417
+00:30:24,230 --> 00:30:27,590
+فحصنا لليوم في هذا الفحص هو ال potassium
+
+418
+00:30:27,590 --> 00:30:34,150
+permanganate KMnO4 potassium permanganate لو حكيتش
+
+419
+00:30:34,150 --> 00:30:38,070
+ال objective لهذا الفحص to oxidize oleic acid by
+
+420
+00:30:38,070 --> 00:30:42,450
+potassium permanganate بيدعم الأكسدة للacid
+
+421
+00:30:42,450 --> 00:30:46,830
+باستخدام البوتاسيوم بيرمانجانات ولو بدك تكتب ال
+
+422
+00:30:46,830 --> 00:30:50,470
+principle على شكل معادلة بحكي ان ال acid اللي انا
+
+423
+00:30:50,470 --> 00:30:54,690
+بده اعمله اكسدا زي ال potassium بيرمانجانات بيحكي
+
+424
+00:30:54,690 --> 00:30:58,930
+اللي بيعطيني لون brownالأولك أسد مع بوتاسيوم
+
+425
+00:30:58,930 --> 00:31:02,990
+بيرمانجانيت العامل المؤكسل يعمل على أكسدة الأولك
+
+426
+00:31:02,990 --> 00:31:06,870
+أسد ويتحول اللون إلى لون براون البوتاسيوم
+
+427
+00:31:06,870 --> 00:31:10,610
+بيرمانجانيت أصلا لونه violet لونه move لونه purple
+
+428
+00:31:10,610 --> 00:31:15,150
+على violet move لما تحطيه على الأولك أسد بيعمل
+
+429
+00:31:15,150 --> 00:31:21,000
+عملية أكسدة للأولك أسد بيحوله إلى لون براونكيف
+
+430
+00:31:21,000 --> 00:31:24,420
+خطوات إجراء التجربة؟ بدي أشوف بالتيوب بدي أشتغل
+
+431
+00:31:24,420 --> 00:31:27,180
+فيها أوليك أسد والتيوب بدي أشتغل فيها استيريك أسد
+
+432
+00:31:27,180 --> 00:31:31,160
+و أشوف من يغير أول تيوب بدي أشتغل فيها أوليك أسد
+
+433
+00:31:31,160 --> 00:31:34,520
+بدي أشتغل فيه one drop of oleic acid على تلاتة مل
+
+434
+00:31:34,520 --> 00:31:38,120
+من ال sodium bicarbonate على اتنين مل من
+
+435
+00:31:38,120 --> 00:31:44,670
+البوتاسيوم بيرمانجنانتمام ده حاط واحد drop من
+
+436
+00:31:44,670 --> 00:31:48,090
+الolic acid على 3 مل من ال sodium bicarbonate على
+
+437
+00:31:48,090 --> 00:31:51,690
+2 مل من ال potassium permanganate الolic acid هو
+
+438
+00:31:51,690 --> 00:31:55,470
+عبارة عن unsaturated fatty acid ييجي ال potassium
+
+439
+00:31:55,470 --> 00:31:59,550
+permanganate بيعمله أكسدة يفترض يتحول اللون إلى
+
+440
+00:31:59,550 --> 00:32:04,390
+brown إذا positive إذا هو olic acidالتيوب التانية
+
+441
+00:32:04,390 --> 00:32:08,470
+بده حط drop من ال stearic acid على 3 مل من ال
+
+442
+00:32:08,470 --> 00:32:12,670
+sodium bicarbonate على 2 مل من ال potassium
+
+443
+00:32:12,670 --> 00:32:16,530
+permanganate بيحكي اللي لما تحطي ال stearic acid
+
+444
+00:32:16,530 --> 00:32:20,150
+هو عبارة عن unsaturated fatty acid فلما تحط عليه
+
+445
+00:32:20,150 --> 00:32:24,550
+ال potassium permanganate بيتغيرش اللون وانما بيظل
+
+446
+00:32:24,550 --> 00:32:31,930
+لونه violet مش هيتغير مش هيتحول للون ل brownأخر
+
+447
+00:32:31,930 --> 00:32:35,790
+فحص هو السلكا ويسكي reaction هذا السلكا ويسكي
+
+448
+00:32:35,790 --> 00:32:41,530
+reaction هو فحص للكشف عن الزيت بستخدم زيت بدي أشوف
+
+449
+00:32:41,530 --> 00:32:45,590
+هل هذا الزيت مخشوش مضعف عليه الكلسترول ولا مش مضعف
+
+450
+00:32:45,590 --> 00:32:51,280
+هذا خاص للكشف هذا الفحص السلكا ويسكي reactionللكشف
+
+451
+00:32:51,280 --> 00:32:55,020
+عن الكوليسترول، هل الكوليسترول موجود ولا لا؟ في
+
+452
+00:32:55,020 --> 00:32:58,320
+ناس بتغش الزيت، بتضيف عليه كوليسترول أنا بدي أشوف
+
+453
+00:32:58,320 --> 00:33:01,380
+هل هذا الزيت مضاف عليه كوليسترول ولا هو زيت زيتون
+
+454
+00:33:01,380 --> 00:33:07,040
+طبيعي؟ كيف مبدأ عمله؟ هذا الفحص مبدأ عمله بيتم على
+
+455
+00:33:07,040 --> 00:33:12,880
+استخدام concentrated sulfuric acid H2SO4 نستخدم
+
+456
+00:33:12,880 --> 00:33:18,420
+concentrated sulfuric acid اللي هو H2SO4هذا حساس
+
+457
+00:33:18,420 --> 00:33:22,900
+للرطوبة «highly hygroscopic» هو حساس للرطوبة لا
+
+458
+00:33:22,900 --> 00:33:25,720
+يمكن للطيوبات اللي أشتغل فيها يكون فيها ماء لازم
+
+459
+00:33:25,720 --> 00:33:31,080
+تكون جافة لأن أصلا مبدأ عمله يعمل على «removal of
+
+460
+00:33:31,080 --> 00:33:33,880
+two molecules of water from two molecules of
+
+461
+00:33:33,880 --> 00:33:38,120
+cholesterol» إن إذا الكوليسترول موجود في الزيتإذا
+
+462
+00:33:38,120 --> 00:33:43,260
+كان في عندي في الزيت موجود كوليسترال بيجي الـH2SO4
+
+463
+00:33:43,260 --> 00:33:46,720
+الـ concentrated sulfuric acid هاد بيقوم بنزع
+
+464
+00:33:46,720 --> 00:33:51,660
+جزيئين ماء من جزيئين كوليسترال إذا في كوليسترال
+
+465
+00:33:51,660 --> 00:33:58,020
+بيجي الـH2SO4 بينزع جزيئين ماء من هذا الكوليسترال
+
+466
+00:33:58,020 --> 00:34:03,310
+من position من موقع تلاتة تلاتةنزع جزيئين ماء من
+
+467
+00:34:03,310 --> 00:34:07,650
+اتنين كلسترول من موقع تلاتة و تلاتة بينتج النتج
+
+468
+00:34:07,650 --> 00:34:10,950
+بيتحول لكلسترول لما تنزع منه جزئين ماء بيتحول
+
+469
+00:34:10,950 --> 00:34:16,050
+لحاجة اسمها bi-cholestadineالـ GTBG كمان مر
+
+470
+00:34:16,050 --> 00:34:20,750
+الصلفوريك أسد هذا بيشتغل على البيكلستادين بيعمله
+
+471
+00:34:20,750 --> 00:34:26,930
+صلفونات بيكلستادين at position 7 و7 بيعمل صلفونات
+
+472
+00:34:26,930 --> 00:34:31,150
+للبيكلستادين بيضيف عليه صلفية ال group بتحول إلى
+
+473
+00:34:31,150 --> 00:34:36,090
+حاجة اسمة bi-sulfonic acid bi-cholestadine لونه
+
+474
+00:34:36,090 --> 00:34:41,200
+red colorإذا بفحص السلكا whisk reaction و فحص
+
+475
+00:34:41,200 --> 00:34:44,420
+للكشف عن الكوليسترول هل موجود في زيتها المضاف عليه
+
+476
+00:34:44,420 --> 00:34:50,260
+كوليسترول ولا لأ؟كيف باستخدام Sulphuric Acid؟ إيش
+
+477
+00:34:50,260 --> 00:34:53,900
+وظيفة Sulphuric Acid في فحص السلكويسكي reaction؟
+
+478
+00:34:53,900 --> 00:34:58,280
+الوظيفتين أول وظيفة remove two molecules of water
+
+479
+00:34:58,280 --> 00:35:02,440
+from two molecules of كوليسترول نزع جزيئين ماء من
+
+480
+00:35:02,440 --> 00:35:06,520
+ال .. من اتنين كوليسترول من موقع تلاتة ويتحول
+
+481
+00:35:06,520 --> 00:35:10,590
+الكوليسترول لحاجة اسمها bi-cholestadineبعدين
+
+482
+00:35:10,590 --> 00:35:13,990
+الوظيفة التانية يعمل صلفونية بيكلستادين at
+
+483
+00:35:13,990 --> 00:35:18,350
+position 7 و7 اللي هي إضافة صلفية ال group على
+
+484
+00:35:18,350 --> 00:35:22,450
+position 7 و7 في البيكلستادين بتكون حاجة اسمها
+
+485
+00:35:22,450 --> 00:35:27,810
+bisulfonic acid bicolestidine لونها red colorإذا
+
+486
+00:35:27,810 --> 00:35:30,650
+ال objective من هذا الفحص to detect the presence
+
+487
+00:35:30,650 --> 00:35:35,890
+of cholesterol كيف مابدأ أعمله؟ إنه بيكون عندى
+
+488
+00:35:35,890 --> 00:35:39,590
+اتنين كلسترول إذا عندى مركبين كلسترول بنحط عليهم
+
+489
+00:35:39,590 --> 00:35:44,590
+concentrated sulfuric acid H2SO4 بيتحول النتج نائي
+
+490
+00:35:44,590 --> 00:35:49,210
+ليش؟ by sulfonate by كوليستادين لونه red
+
+491
+00:35:54,630 --> 00:35:58,110
+هنا عملنا structure لبيكولستادين كيف شكله و
+
+492
+00:35:58,110 --> 00:36:02,390
+بيسالفونك أسد بيكولستادين كيف شكله عشان أطلب مننا
+
+493
+00:36:02,390 --> 00:36:07,650
+ال structureإيش ال .. كيف خطوات التجربة أو إيش
+
+494
+00:36:07,650 --> 00:36:10,670
+نستخدم ال agent نستخدم زيت زتون مضاعف عليه
+
+495
+00:36:10,670 --> 00:36:15,030
+الكوليسترول نستخدم زيت زتون طبيعي و زيت زتون مضاعف
+
+496
+00:36:15,030 --> 00:36:18,430
+عليه الكوليسترول عشان نشوف الفان و نستخدم ال
+
+497
+00:36:18,430 --> 00:36:22,330
+chloroform عشان ندوّب بس الزيت عشان أقدر أدوّب
+
+498
+00:36:22,330 --> 00:36:28,350
+الزيت و نتجنب استنشاق كمية من هذا ال chloroform
+
+499
+00:36:28,350 --> 00:36:31,550
+لأن هذا بيأثر على ال central nervous system على
+
+500
+00:36:31,550 --> 00:36:37,080
+الجهاز العصبي بسبب نازيا و headacheو business صداع
+
+501
+00:36:37,080 --> 00:36:43,900
+و غثيان و كوم داخل و بدنا نستخدم ال sulfuric acid
+
+502
+00:36:43,900 --> 00:36:48,640
+اللي له وظيفتين في هذا الفحص حكينا عنهم كيف طريقة
+
+503
+00:36:48,640 --> 00:36:52,920
+إجراء التجربة ده جيب كيبين واحد استغل فيها الزيت
+
+504
+00:36:52,920 --> 00:36:56,620
+الزيتون من غير كلسترول و واحدة بدأ استخدم فيها زيت
+
+505
+00:36:56,620 --> 00:37:00,920
+زيتون مضاف عليه كلسترول عشان أشوف الفانأول تيوب
+
+506
+00:37:00,920 --> 00:37:07,920
+سأضع عليها 1ml كلوروفارم 1ml كلوروفارم على 2 drop
+
+507
+00:37:07,920 --> 00:37:13,720
+من الزيت 1ml كلوروفارم على 2 drop من الزيت ثم أضع
+
+508
+00:37:13,720 --> 00:37:18,300
+2ml من H2اسوفارم هجد زيت زيتون إذا كان طبيعي مش
+
+509
+00:37:18,300 --> 00:37:22,340
+هيتغير إشي وهيكون negative في الشلون تيوب التاني
+
+510
+00:37:22,340 --> 00:37:27,400
+سأضع عليها 1ml كلوروفارمو اتنين drop من
+
+511
+00:37:27,400 --> 00:37:30,820
+الكوليسترول زيت زيتون مضاف عليه كوليسترول و احط
+
+512
+00:37:30,820 --> 00:37:35,460
+اتنين مل من H2SO فان بلاحظ ان اللون عندى بيتحول
+
+513
+00:37:35,460 --> 00:37:39,620
+الى red اذا اتكونت ال red اللون الأحمر اذا هذا
+
+514
+00:37:39,620 --> 00:37:47,590
+الزيت مضاف عليه كوليسترولإذا ما اتحولش إذا هو زيت
+
+515
+00:37:47,590 --> 00:37:51,410
+زتون طبيعي بدون كوليسترول بيحكيلي في أول إشي انه
+
+516
+00:37:51,410 --> 00:37:56,050
+انه تكون ال dry test tube تكون لتيوبات جافة ضروري
+
+517
+00:37:56,050 --> 00:37:59,190
+لتيوبات تكون جافة لأنه هذا حكينا أنه highly
+
+518
+00:37:59,190 --> 00:38:03,530
+hygroscopic حساس للرطوبة لأن أصلا المبدأ عامله أنه
+
+519
+00:38:03,530 --> 00:38:07,350
+بده جزيئين ما ينزع جزيئين ماإذا التيوب فيها مي
+
+520
+00:38:07,350 --> 00:38:11,250
+هياخد المي الموجودة في التيوب مش هيشتغل على
+
+521
+00:38:11,250 --> 00:38:15,510
+الكوليسترول إذا موجود انما هيشتغل على المي اللي في
+
+522
+00:38:15,510 --> 00:38:21,410
+التيوب بدل الكوليسترول هيك بنكون خلصنا هذا المعمل،
+
+523
+00:38:21,410 --> 00:38:25,010
+المعمل الليبد ان شاء الله نشتغل عملي فيه المعمل،
+
+524
+00:38:25,010 --> 00:38:30,680
+مطلوب منكمتكتبولي ال principle لكل فحص على شكل
+
+525
+00:38:30,680 --> 00:38:35,640
+معادلات و اللي مش معادلة تكتبولي المبدأ برضه
+
+526
+00:38:35,640 --> 00:38:37,080
+يعطيكم العافية
+
diff --git a/PL9fwy3NUQKwZ1Q7zOg1ObgufPMjRI3WY1/ejpJCjHaqTg.srt b/PL9fwy3NUQKwZ1Q7zOg1ObgufPMjRI3WY1/ejpJCjHaqTg.srt
new file mode 100644
index 0000000000000000000000000000000000000000..2962690c8ebc02481d1fdd9787c111ef0496456b
--- /dev/null
+++ b/PL9fwy3NUQKwZ1Q7zOg1ObgufPMjRI3WY1/ejpJCjHaqTg.srt
@@ -0,0 +1,1392 @@
+1
+00:00:00,000 --> 00:00:04,920
+بنحكي في موضوع جديد هو موضوع الفيتامينات، أتحدث عن
+
+2
+00:00:04,920 --> 00:00:10,570
+الفيتامينات وعن أقسام الفيتامينات، أول شيء نحكي عن 
+
+3
+00:00:10,570 --> 00:00:15,190
+خصائص الvitamins، characteristics of vitamins، أول شيء 
+
+4
+00:00:15,190 --> 00:00:19,930
+الvitamins هي عبارة عن micro nutrients، هي مغذيات 
+
+5
+00:00:19,930 --> 00:00:24,530
+صغيرة، أول خاصية أنها تعتبر micro nutrients، مغذيات 
+
+6
+00:00:24,530 --> 00:00:29,530
+صغيرة، ليش بيعتبرها micro؟ لأن very small amount are 
+
+7
+00:00:29,530 --> 00:00:34,620
+needed by the body، الجسم بيحتاجها بكميات قليلة، very 
+
+8
+00:00:34,620 --> 00:00:38,820
+small amount are contained in food، و الأطعمة تحتوي 
+
+9
+00:00:38,820 --> 00:00:43,380
+على الفيتامينات بكميات قليلة، إذن هي مغذيات صغيرة 
+
+10
+00:00:43,380 --> 00:00:47,840
+لأنه بيحتاجها الجسم بكميات قليلة، وكمان موجودة في 
+
+11
+00:00:47,840 --> 00:00:52,360
+الأطعمة بكميات قليلة، بعد هيك الvitamins are 
+
+12
+00:00:52,360 --> 00:00:57,160
+essential، الفيتامينات ضرورية ومهمة للجسم لأنها 
+
+13
+00:00:57,160 --> 00:01:02,100
+بتلعب دور مهم في الجسم، play important role in the 
+
+14
+00:01:02,100 --> 00:01:06,380
+body، most vitamins are obtained from the food we 
+
+15
+00:01:06,380 --> 00:01:10,680
+eat، من الطعام، الجسم بياخد الفيتامينات معظم 
+
+16
+00:01:10,680 --> 00:01:15,260
+الفيتامينات بياخدها من الطعام، الطعام اللي بيتناوله
+
+17
+00:01:15,260 --> 00:01:20,790
+بيحتوي على الفيتامينات، في بعض الفيتامينات بتصنعها 
+
+18
+00:01:20,790 --> 00:01:25,870
+بكتيريا موجودة في الأمعاء زي الnormal flora، بتصنع 
+
+19
+00:01:25,870 --> 00:01:30,680
+vitamin K، بعض الفيتامينات بتصنعها بكتيريا موجودة 
+
+20
+00:01:30,680 --> 00:01:35,260
+فيها، لما و أما معظم الفيتامينات بناخدها من الطعام 
+
+21
+00:01:35,260 --> 00:01:38,600
+there is no perfect food that contain all the
+
+22
+00:01:38,600 --> 00:01:43,000
+vitamins in the right amount، فش طعام واحد مثلًا
+
+23
+00:01:43,000 --> 00:01:47,820
+أقول لك الطماطم تحتوي على كل أنواع الفيتامينات، لأ 
+
+24
+00:01:47,820 --> 00:01:51,880
+الفيتامين منتشر في أطعمة مختلفة، لكن فش طعام واحد 
+
+25
+00:01:51,880 --> 00:01:53,980
+يحتوي كل أنواع الفيتامين
+
+26
+00:01:58,030 --> 00:02:02,990
+هي الفيتامينات لا تحتوي سعرات حرارية، not contain 
+
+27
+00:02:02,990 --> 00:02:06,470
+kilo calories، لا تحتوي سعرات حرارية، وبالتالي هي 
+
+28
+00:02:06,470 --> 00:02:12,270
+لا تعطي طاقة ولا تنتج الطاقة، vitamins are 
+
+29
+00:02:12,270 --> 00:02:15,270
+classified according to how soluble they are in 
+
+30
+00:02:15,270 --> 00:02:19,530
+fat or water، الفيتامينات مقسمة حسب دوبانيتها، في 
+
+31
+00:02:19,530 --> 00:02:24,980
+منها تدوب في الدهون، وفي منها تدوب في الماء، عندنا هذا
+
+32
+00:02:24,980 --> 00:02:28,960
+الجدول بيفرق بين الvitamins اللي هم على قسمين
+
+33
+00:02:28,960 --> 00:02:32,440
+العمودي الأول اللي هي الwater soluble vitamins 
+
+34
+00:02:32,440 --> 00:02:37,140
+وفي عندنا fat soluble vitamins، أنثى على water 
+
+35
+00:02:37,140 --> 00:02:41,860
+soluble vitamins، عندنا vitamin B وC، أما الfat 
+
+36
+00:02:41,860 --> 00:02:48,160
+soluble vitamins، عندنا vitamin A، D، E، و K، مجموعين في
+
+37
+00:02:48,160 --> 00:02:53,920
+كلمة "أَكِيد" أو "أَدِيك"، لذا الفات سوليوبل في عندنا، و
+
+38
+00:02:53,920 --> 00:02:57,160
+في عندنا water soluble vitamins، الwater soluble هي 
+
+39
+00:02:57,160 --> 00:03:03,550
+الB و الC، الفات سوليوبل هم A وD وE وK، طيب
+
+40
+00:03:03,550 --> 00:03:06,570
+نيجي نفرق بينهم أول شيء من ناحية الabsorption
+
+41
+00:03:06,570 --> 00:03:11,370
+مدام هو water soluble vitamins، الwater soluble 
+
+42
+00:03:11,370 --> 00:03:15,690
+vitamins، يعني بتدوب في المي، والجسم على طول أغلب 
+
+43
+00:03:15,690 --> 00:03:19,730
+مكوناته مي، فهي على طول directly into the blood، بتم 
+
+44
+00:03:19,730 --> 00:03:24,970
+إمتصاصها مباشرة عبر الدم، أما الفات سوليوبل لازم
+
+45
+00:03:24,970 --> 00:03:29,510
+بأول تروح على الlymph، وبعدين تيجي على الدم عشان
+
+46
+00:03:29,510 --> 00:03:35,190
+يتم امتصاصها، وهي تنقلها، إذًا الامتصاص الwater 
+
+47
+00:03:35,190 --> 00:03:38,590
+soluble يتم امتصاصها مباشرة في الدم، أما الفات 
+
+48
+00:03:38,590 --> 00:03:42,370
+soluble vitamins لازم تروح على اللف وبعدين يتم
+
+49
+00:03:42,370 --> 00:03:48,380
+نقلها للدم، طيب transport كيف بدها تتنقل هاي ال
+
+50
+00:03:48,380 --> 00:03:52,140
+water soluble؟ مدام ما خصائصها زي خصائص الجسم، فهي
+
+51
+00:03:52,140 --> 00:03:56,260
+travel freely، بتنتقل بحرية، بتمشي بحرية من غير أي 
+
+52
+00:03:56,260 --> 00:04:00,200
+معوّقات، أما الfat soluble vitamins لأن خصائصها 
+
+53
+00:04:00,200 --> 00:04:05,270
+بتختلف عن الجسم، فبتحتاج إلى نواقل require carrier 
+
+54
+00:04:05,270 --> 00:04:09,030
+proteins، بتحتاج إلى نواقل عشان تتنقلها وعشان تمشي
+
+55
+00:04:09,030 --> 00:04:13,590
+في داخل الجسم، الstorage التخزين، هل بيتم تخزينها 
+
+56
+00:04:13,590 --> 00:04:18,430
+ولا لا، الwater soluble vitamins بتم تخزينها، بدل 
+
+57
+00:04:18,430 --> 00:04:22,150
+هذا، كل اللي مكتوب circulated freely، لنكتب not 
+
+58
+00:04:22,150 --> 00:04:27,150
+stored، لا تخزن، ما بيتم تخزينها، أما الfat soluble 
+
+59
+00:04:27,150 --> 00:04:31,240
+vitamins بتم تخزينها، it is stored when بتخزن في 
+
+60
+00:04:31,240 --> 00:04:36,400
+الأنسجة الدهنية، في الخلايا الدهنية، حسنًا، طبيعة
+
+61
+00:04:36,400 --> 00:04:40,480
+خلايا الجسم دهنية، فهتتخزن الfat soluble vitamins 
+
+62
+00:04:40,480 --> 00:04:45,060
+اللي بتشبهها، إذا الwater لا تخزن، أما الفات تخزن
+
+63
+00:04:45,060 --> 00:04:50,390
+excretion، هجينا الإخراج، مدام الwater لا تخزن في
+
+64
+00:04:50,390 --> 00:04:55,710
+الجسم، فاللي هيكون زائد عن حاجة الجسم هيتم إخراجه
+
+65
+00:04:55,710 --> 00:05:00,530
+الkidney هتعمل الdetection هتعمله فلترة وتعمله
+
+66
+00:05:00,530 --> 00:05:04,130
+remove excess in urine، وهتعمله إخراج مع الurine 
+
+67
+00:05:04,130 --> 00:05:08,330
+الexcretion معناه الإخراج، الwater soluble 
+
+68
+00:05:08,330 --> 00:05:12,340
+vitamins، مدام مافيش تخزين لها، مش هيتخزن في الجسم 
+
+69
+00:05:12,340 --> 00:05:16,960
+فالكمية الزائدة عن حاجة الجسم هيتم إخراجها مع
+
+70
+00:05:16,960 --> 00:05:21,660
+اليورين، أما الفات سوليوبل vitamins مدامها بتخزن إذا
+
+71
+00:05:21,660 --> 00:05:26,620
+الكمية الزائدة عن حاجة الجسم بتم تخزينها في
+
+72
+00:05:26,620 --> 00:05:30,880
+الأنسجة والخلايا الدهنية، إذا في الexecution في
+
+73
+00:05:30,880 --> 00:05:34,360
+الفوتر سوليوبل بتم execution، أما في الفات no
+
+74
+00:05:34,360 --> 00:05:40,650
+execution،  الtoxicity اللي هو السمية، أنه يوصل لحد
+
+75
+00:05:40,650 --> 00:05:44,490
+السمية، كمية الفيتامين في الجسم صارت زيادة، كمية
+
+76
+00:05:44,490 --> 00:05:49,710
+كبيرة ووصلت لحد السمية، هلجينا الwater soluble
+
+77
+00:05:49,710 --> 00:05:52,470
+possible to reach toxic level؟
+
+78
+00:05:54,740 --> 00:05:59,740
+الـtoxicity، حكينا السمية، أن يوصل لحد السمية، ال
+
+79
+00:05:59,740 --> 00:06:03,000
+water soluble بتتم إخراجها أصلاً خارج الجسم، فممكن 
+
+80
+00:06:03,000 --> 00:06:08,720
+توصل لحد السمية، ولكن يعني احتمال قليل، وبيحكي إن
+
+81
+00:06:08,720 --> 00:06:12,440
+هذه الtoxicity بتيجي من consumed from supplement
+
+82
+00:06:12,440 --> 00:06:17,840
+لما ياخد supplement مدعمات أو أقراص vitamins ممكن
+
+83
+00:06:17,840 --> 00:06:21,580
+هنا نوصل لحد السمية، في الfat soluble vitamins 
+
+84
+00:06:21,910 --> 00:06:26,890
+السمية عالية، احتمال أنه نوصل لحد السمية likely، ليش 
+
+85
+00:06:26,890 --> 00:06:31,290
+لأنه أصلاً بيخزن في الجسم، طب الrequirements اللي هو
+
+86
+00:06:31,290 --> 00:06:34,750
+حاجة الجسم إلها، من جداش لجداش الفترة، أنا لازم
+
+87
+00:06:34,750 --> 00:06:38,630
+أتناول هذه الفيتامينة، الwater soluble vitamins 
+
+88
+00:06:38,630 --> 00:06:43,450
+بنحتاجه frequent dose، يعني من كل يوم لتلت أيام بدي 
+
+89
+00:06:43,450 --> 00:06:47,710
+منه، لأنه بيتم إخراجه، لكن الfat soluble لأنه بيتم 
+
+90
+00:06:47,710 --> 00:06:52,770
+تخزينه في داخل الجسم، ففترات متباعدة خلال periodic
+
+91
+00:06:52,770 --> 00:06:58,240
+dose، ممكن من أسبوع لشهر، أحتاج الfat soluble 
+
+92
+00:06:58,240 --> 00:07:01,820
+vitamins، إذا هنا عندنا نوعين من أنواع الvitamins 
+
+93
+00:07:01,820 --> 00:07:06,220
+water soluble و الfat soluble vitamins، خدنا مثال 
+
+94
+00:07:06,220 --> 00:07:09,760
+على هاي، ومثال على هاي، ومقارنة بينهم، فالجاي هنفصل 
+
+95
+00:07:09,760 --> 00:07:14,040
+كل واحدة، أول شيء إن نحكي عن الfat soluble vitamins
+
+96
+00:07:14,040 --> 00:07:19,220
+اللي حكينا هم أربعة، مجموعين في كلمة أَكِيد أو أَدِيك
+
+97
+00:07:20,670 --> 00:07:23,910
+الخصائص العامة للfats, soluble vitamins، هذا ملخص 
+
+98
+00:07:23,910 --> 00:07:26,550
+الجدول اللي خاص في الfats, soluble vitamins 
+
+99
+00:07:26,550 --> 00:07:30,490
+الأطعمة اللي بتكون موجودة فيها الفات و الsoluble
+
+100
+00:07:30,490 --> 00:07:33,290
+vitamins، بتكون موجودة في الأطعمة الدهنية، وفي 
+
+101
+00:07:33,290 --> 00:07:38,050
+الزيت، in fats and oil، require piles for absorption
+
+102
+00:07:38,050 --> 00:07:42,490
+بتحتاج.. لازم نحتاج حاجة عشان تساعد على امتصاصها 
+
+103
+00:07:42,490 --> 00:07:47,450
+حكينا فبتحتاج الpiles للامتصاص، إذا فاكرين حكينا
+
+104
+00:07:47,450 --> 00:07:51,350
+في معمل الcholesterol، أنه الbile اللي بتشتغل ك
+
+105
+00:07:51,350 --> 00:07:56,530
+soap، كصابونة بتعمل على امتصاص الفات، كيف بده ينهضم
+
+106
+00:07:56,530 --> 00:08:01,380
+في الجسم، ويتم امتصاصه عبر الbile، Enter the lymph 
+
+107
+00:08:01,380 --> 00:08:05,220
+in the blood، تدخل الليمف في العقد وبعدين بتروح على 
+
+108
+00:08:05,220 --> 00:08:09,980
+الدم، وبتم تخزينها في fatty tissues، الخلايا الدهنية 
+
+109
+00:08:09,980 --> 00:08:15,120
+need in small amount، وبنحتاجها بكميات قليلة، ممكن 
+
+110
+00:08:15,120 --> 00:08:20,200
+نوصل لحد السمية، لأنه فش إلها إخراج not readily 
+
+111
+00:08:20,200 --> 00:08:22,460
+executed، ما بتم إخراجها 
+
+112
+00:08:25,100 --> 00:08:28,700
+طب أول نوع من أنواع الفات سوليوبل فيتامين، فيتامين
+
+113
+00:08:28,700 --> 00:08:34,700
+A، فيتامين A، في منه تلات أشكال، تلات أشكال من فيتامين
+
+114
+00:08:34,700 --> 00:08:39,520
+A موجود في الجسم على تلات أشكال، ريتينول، ريتينال 
+
+115
+00:08:39,520 --> 00:08:45,060
+ريتينويك أسيد، إذا الفات الـvitamin A موجود في الجسم
+
+116
+00:08:45,060 --> 00:08:51,210
+على تلات أشكال، ريتينول، ريتينال، retinoic acid، كلهم مع 
+
+117
+00:08:51,210 --> 00:08:55,390
+بعض collectively known as retinoid، كلهم مع بعض
+
+118
+00:08:55,390 --> 00:09:00,170
+بنسميهم retinoid، الprecursor اللي هي الوحدة
+
+119
+00:09:00,170 --> 00:09:03,910
+البنائية في تكوين vitamin A، في تكوين الretinoid 
+
+120
+00:09:03,910 --> 00:09:09,830
+الوحدة الأساسية والبنائية هو الbeta carotene، ال
+
+121
+00:09:09,830 --> 00:09:15,330
+beta carotene هو الوحدة البنائية لتكوين vitamin A
+
+122
+00:09:17,900 --> 00:09:21,400
+derived from plant food، من وين بدي أحصل على هذا ال
+
+123
+00:09:21,400 --> 00:09:25,040
+beta carotene، عشان يكون لفيتامين a، يكون لريتينول
+
+124
+00:09:25,040 --> 00:09:29,500
+ريتينويد، بحصل عليه من الplant food، من الأطعمة
+
+125
+00:09:29,500 --> 00:09:34,180
+النباتية، وكمان can split and form retinol in
+
+126
+00:09:34,180 --> 00:09:38,500
+intestine and liver، وبتم تصنيع في الliver وال
+
+127
+00:09:38,500 --> 00:09:43,240
+intestine، البيتا كاروتين one، الأطعمة بنلاقيه في
+
+128
+00:09:43,240 --> 00:09:48,060
+الخضروات الورقية الخضراء، بنلاقيه في الخضروات اللي 
+
+129
+00:09:48,060 --> 00:09:53,140
+لونها برتقالي، وفي الفواكه اللي هو البرتقال، ال
+
+130
+00:09:53,140 --> 00:09:57,580
+orange fruit، vitamin
+
+131
+00:09:57,580 --> 00:10:01,860
+A function، إيش وظيفة vitamin A؟ أهم وظيفة لvitamin 
+
+132
+00:10:01,860 --> 00:10:05,520
+A، الvision اللي هو في النظر، في الرؤية، يستخدم في
+
+133
+00:10:05,520 --> 00:10:10,210
+الرؤية، maintain epithelial tissue and skin، بحافظ 
+
+134
+00:10:10,210 --> 00:10:14,810
+على الأنسجة والخلايا، support reproduction and
+
+135
+00:10:14,810 --> 00:10:19,830
+growth، بيدعم النمو والبلوغ، بساعد في النمو و
+
+136
+00:10:19,830 --> 00:10:24,890
+البلوغ، immune system، الوظائف في جهاز المناعة، bone
+
+137
+00:10:24,890 --> 00:10:31,270
+development، الوظائف في نمو وتطور العظم
+
+138
+00:10:33,520 --> 00:10:37,340
+هلجينا vitamin A deficiency، اللي هي النقص، هلجينا هل
+
+139
+00:10:37,340 --> 00:10:40,640
+في احتمال؟ إيش احتماليته إنه يتم نقص vitamin A في
+
+140
+00:10:40,640 --> 00:10:44,680
+الجسم؟ الاحتمال إنه يتم نقص vitamin A هو احتمال
+
+141
+00:10:44,680 --> 00:10:48,680
+ضعيف، لأنه حكينا إن vitamin A من الفات سوليو
+
+142
+00:10:48,680 --> 00:10:52,380
+بالفيتامين، والفات سوليو بالفيتامين بتتم تخزينها
+
+143
+00:10:52,380 --> 00:10:56,200
+في الخلايا الدهنية في الجسم، ما بتتم إخراجها
+
+144
+00:10:56,200 --> 00:11:00,740
+فاحتمال النقص احتمال ضعيف، لأنه بتم تخزينه في الجسم 
+
+145
+00:11:00,740 --> 00:11:05,580
+فهو rare، الdeficiency rare، لكن إذا صار deficiency،
+
+146
+00:11:05,580 --> 00:11:09,860
+إيش بيسبب أمراض؟ بسبب infectious disease، أمراض 
+
+147
+00:11:09,860 --> 00:11:14,460
+معديّة، زي الـpneumonia اللي هي الالتهاب الرئوي،
+
+148
+00:11:14,460 --> 00:11:20,510
+measles، حصبة، ودياريا، وكمان ممكن يسبب
+
+149
+00:11:20,510 --> 00:11:24,970
+keratinization، لإنه حكينا إنه من وظائف vitamin A 
+
+150
+00:11:24,970 --> 00:11:29,370
+إنه بيحافظ على الأنسجة والخلايا الجلد، فبيصير إذا
+
+151
+00:11:29,370 --> 00:11:34,130
+نقص هيصير keratinization اللي هو تأثير على 
+
+152
+00:11:34,500 --> 00:11:38,400
+keratinization، يعني هيصير تأثير على الجلد، بيصير 
+
+153
+00:11:38,400 --> 00:11:42,900
+تراكم للكيراتين تحت الجلد، تراكم للكيراتين تحت
+
+154
+00:11:42,900 --> 00:11:47,140
+الجلد، فبيصير الجلد عبارة عن الdry، جاف وrough 
+
+155
+00:11:47,140 --> 00:11:51,080
+بيكون خشن، وبيكون scaly، جلد باهت، لذا ال
+
+156
+00:11:51,080 --> 00:11:54,560
+keratinization، تراكم الكيراتين تحت الجلد، فبيكون
+
+157
+00:11:54,560 --> 00:12:00,280
+الجلد باهت وخشن وجاف، وكمان فقد أو ديفشانس ل
+
+158
+00:12:00,280 --> 00:12:04,970
+vitamin A بيعمل night blindness، اللي هو العشى
+
+159
+00:12:04,970 --> 00:12:10,310
+الليلي، إن الرؤية بالليل بتكون خافتة، طب نيجي 
+
+160
+00:12:10,310 --> 00:12:13,270
+للفيتامين التاني من الفات سوليو بالفيتامين، اللي هو 
+
+161
+00:12:13,270 --> 00:12:18,670
+فيتامين دال، فيتامين دال بيصنع في الجسم، body can make
+
+162
+00:12:18,670 --> 00:12:23,070
+فيتامين دال من أشعة الشمس، بياخد من أشعة الشمس و
+
+163
+00:12:23,070 --> 00:12:26,570
+بيقوم فيتامين دال، وكمان حكينا قبل هيك أنه
+
+164
+00:12:26,570 --> 00:12:31,670
+الكلسترول يعتبر الوحدة البنائية لتكوين فيتامين دال 
+
+165
+00:12:31,670 --> 00:12:35,690
+إذا ففيتامين دال بياخده من أشعة الشمس، ومن
+
+166
+00:12:35,690 --> 00:12:40,570
+الكلسترول، بتم تصنيعه في الكلية والكبد، in liver
+
+167
+00:12:40,570 --> 00:12:45,410
+and kidney، لو صار عندي أي مرض في الliver وال
+
+168
+00:12:45,410 --> 00:12:49,150
+kidney، هذا هيأثر على الactivation، على تكوين
+
+169
+00:12:49,150 --> 00:12:53,610
+فيتامين دال، لذن إذا صار أي مرض في الكلية والكبد
+
+170
+00:12:53,610 --> 00:12:59,470
+هيأثر على إنتاج فيتامين دال، source of vitamin دال
+
+171
+00:12:59,470 --> 00:13:03,570
+لون الليمون، موجود في vitamin دال، لون موجود؟ موجود
+
+172
+00:13:03,570 --> 00:13:07,310
+في الfortified food، في الملك، في الmargarine، في 
+
+173
+00:13:07,310 --> 00:13:10,750
+الbeef، في الegg، في الsun، من أشعة الشمس، في
+
+1
+
+201
+00:15:16,440 --> 00:15:19,800
+يعني stimulating retention؟  الـ retention أني أسحب
+
+202
+00:15:19,800 --> 00:15:23,900
+الكالسيوم و الفوسفرس من الكلية للدم بدل ما يتم 
+
+203
+00:15:23,900 --> 00:15:29,400
+إخراجه مع البول لكي الـ kidney الكلية توصلها فضلات
+
+204
+00:15:29,400 --> 00:15:32,570
+و بقايا الجسم اللي بتعملها يخرج مع الـ urine قبل ما
+
+205
+00:15:32,570 --> 00:15:37,010
+تعمل إخراج هيب يعمل فيتامين دال على الـ retention
+
+206
+00:15:37,010 --> 00:15:41,370
+أنه يسحب الكالسيوم و الفوسفورس من الكلية إلى الدم
+
+207
+00:15:41,370 --> 00:15:47,060
+يرجع ثاني بدل ما يتم إخراجه مع الـ bone deficiency
+
+208
+00:15:47,060 --> 00:15:51,500
+لو قال فيتامين دال أهم الأسباب الأمراض اللي هتصير
+
+209
+00:15:51,500 --> 00:15:56,340
+أنه هيأثر على العظم هو بيصير infection بيصير عندي
+
+210
+00:15:56,340 --> 00:16:01,480
+عدوى و أهم شيء أنه بيصير عندي ostomalgia أو ركيزيا
+
+211
+00:16:01,480 --> 00:16:04,960
+إيش هي الـ ostomalgia و الركيزيا؟ هذا الكساح
+
+212
+00:16:04,960 --> 00:16:09,480
+الركيزيا هو الكساح عند الـ child و الـ infant عند 
+
+213
+00:16:09,480 --> 00:16:15,190
+الصغار السن يطلق على الكساح عند صغار السن ركيزيا
+
+214
+00:16:15,190 --> 00:16:20,210
+أوستيو ماليزيا هو الكساح عند كبار السن عند الـ
+
+215
+00:16:20,210 --> 00:16:26,550
+adult بيكون عند الـ adult طب نحكي عن فيتامين E ثالث
+
+216
+00:16:26,550 --> 00:16:30,850
+واحد من الفئة الـ fat soluble vitamin هو فيتامين E فيتامين 
+
+217
+00:16:30,850 --> 00:16:36,630
+E يعتبر antioxidant يعتبر antioxidant إيش يعني
+
+218
+00:16:36,630 --> 00:16:42,970
+antioxidant؟ Antioxidant يعني ضد الأكسدة ضد الأكسدة
+
+219
+00:16:42,970 --> 00:16:47,230
+ضد الأكسدة يعني بتبطئ عملية الأكسدة Antioxidant
+
+220
+00:16:47,230 --> 00:16:49,950
+is a molecule that inhibit the oxidation of
+
+221
+00:16:49,950 --> 00:16:53,570
+another molecule بتبطئ عملية الأكسدة Defender 
+
+222
+00:16:53,570 --> 00:16:57,270
+against free radical كيف هي الـ antioxidant بتكون 
+
+223
+00:16:57,270 --> 00:17:01,950
+ضرورية للجسم؟ إنها بتدافع عن الجسم ضد الـ free
+
+224
+00:17:01,950 --> 00:17:06,390
+radical إيش هي الـ free radical أنه لما يدخل فيروس
+
+225
+00:17:06,390 --> 00:17:10,130
+أو بكتيريا للجسم لداخل الجسم يدخل كأن غريب زي
+
+226
+00:17:10,130 --> 00:17:14,270
+فيروس أو بكتيريا الجسم بيروح بيقاوم هذا الفيروس أو
+
+227
+00:17:14,270 --> 00:17:19,100
+بكتيريا كأنه بيقاومه وبينتج الـ free radical free
+
+228
+00:17:19,100 --> 00:17:22,820
+radical هي دي عشان يتهاجم الفيروس و البكتيريا لكن
+
+229
+00:17:22,820 --> 00:17:26,860
+عيوبها أنها بتهاجم كمان خلايا الجسم هي هتهاجم 
+
+230
+00:17:26,860 --> 00:17:30,900
+الفيروس و البكتيريا و تقضي عليه و تهاجم كمان خلايا 
+
+231
+00:17:30,900 --> 00:17:36,520
+الجسم يعني هتضر خلايا الجسم فإيش بيجي vitamin E
+
+232
+00:17:36,520 --> 00:17:41,140
+بيعمل اختزال لهذه الـ free radical هو ضد الـ X ده فإيش 
+
+233
+00:17:41,140 --> 00:17:45,960
+هيعمل اختزال بيختزل الـ free radical و بيوقف نشاطها
+
+234
+00:17:47,260 --> 00:17:51,680
+فهو Defender against free radical بيدافع عن الجسم
+
+235
+00:17:51,680 --> 00:17:55,520
+من الـ free radical عشان ما تأثرش عليه may reduce 
+
+236
+00:17:55,520 --> 00:18:00,020
+the risk of heart disease بيقلل خطر الإصابة بأمراض
+
+237
+00:18:00,020 --> 00:18:04,540
+القلب widespread in food منتشر بشكل واسع في أطعمة
+
+238
+00:18:04,540 --> 00:18:09,760
+كثيرة easily destroyed by heat processing الحرارة
+
+239
+00:18:09,760 --> 00:18:14,920
+بتأثر عليه vitamin E الحرارة بتأثر عليه وحساس
+
+240
+00:18:14,920 --> 00:18:20,120
+للحرارة بنطلق عليه heat sensitive الـ deficiency الـ
+
+241
+00:18:20,120 --> 00:18:24,000
+deficiency له برضه rare نادرة ما يحدث deficiency
+
+242
+00:18:24,000 --> 00:18:29,040
+لأنه هو عبارة عن fat soluble vitamins بتم تخزين الـ
+
+243
+00:18:29,040 --> 00:18:33,160
+excess منه بتم تخزينه في الجسم فإن يفقدة أو أنه 
+
+244
+00:18:33,160 --> 00:18:38,650
+يتم نقص فيه هذا نادرًا إيه لو صار deficiency
+
+245
+00:18:38,650 --> 00:18:42,170
+مرض ممكن يصير بيصير عندي erythrocyte hemolysis
+
+246
+00:18:42,170 --> 00:18:47,670
+erythrocyte hemolysis هي تكسير لخلايا الدم الحمراء
+
+247
+00:18:47,670 --> 00:18:56,550
+تعني تكسير لخلايا الدم الحمراء vitamin K vitamin K 
+
+248
+00:18:56,550 --> 00:19:00,250
+برضه fat-soluble vitamin aid in blood clotting
+
+249
+00:19:00,250 --> 00:19:04,750
+and bone mineralization بيلعب دور في تجلط الدم
+
+250
+00:19:05,020 --> 00:19:11,380
+وتمعدن العظم دور vitamin K بيلعب دور في تجلط الدم 
+
+251
+00:19:11,380 --> 00:19:16,040
+وتمعدن العظم deficiency causes hemorrhagic disease
+
+252
+00:19:16,040 --> 00:19:22,480
+إذا صار فيه نقص هيصير أمراض نزيفية أمراض نزف ليش
+
+253
+00:19:22,480 --> 00:19:25,680
+لأنه دوره أنه يلعب دور في تجلط الدم وتخسر الدم إذا
+
+254
+00:19:25,680 --> 00:19:30,190
+نقص ب يصير أمراض نزيف مش تجلط للدم فيه نزيف source
+
+255
+00:19:30,190 --> 00:19:34,610
+من وين بنحصل على vitamin K هو vitamin بتم تصنيعه
+
+256
+00:19:34,610 --> 00:19:37,450
+من البكتيريا الموجودة في اللمعاء من الـ
+
+257
+00:19:37,450 --> 00:19:41,990
+gastrointestinal tract و بتم امتصاصه وتخزينه في الـ
+
+258
+00:19:41,990 --> 00:19:47,050
+liver إذا مصدره من بكتيريا موجودة في اللمعاء و بتم
+
+259
+00:19:47,050 --> 00:19:52,270
+امتصاصه وتخزينه في الـ liver بيستخدم لتصنيع عوامل
+
+260
+00:19:52,270 --> 00:19:57,630
+التجلط الـ clothing factor هكذا بنقول خلصنا الـ fat
+
+261
+00:19:57,630 --> 00:20:01,130
+soluble vitamin وحكينا عن كل واحد منهم على السريع
+
+262
+00:20:01,130 --> 00:20:03,810
+اللي هنجي على القسم الثاني اللي هم الـ water
+
+263
+00:20:03,810 --> 00:20:08,170
+soluble vitamin الـ water soluble vitamin هم
+
+264
+00:20:08,170 --> 00:20:13,590
+vitamin B و C بس B بيحكوا عنها B complex لأنهم
+
+265
+00:20:13,590 --> 00:20:19,010
+عبارة عن 8 أنواع من B بس بنطلق عليهم باسم B كلهم B 
+
+266
+00:20:19,010 --> 00:20:23,630
+لكن هم B complex عددهم 8 مع vitamin C عن الـ water
+
+267
+00:20:23,630 --> 00:20:29,450
+soluble vitamin بيطلعوا 9 خصائص الـ water soluble
+
+268
+00:20:29,450 --> 00:20:33,890
+vitamins اللي حكيناها في الجدول إنها لا تلعب دور
+
+269
+00:20:33,890 --> 00:20:38,870
+في إنتاج الطاقة إنها هي non energy producing لا
+
+270
+00:20:38,870 --> 00:20:45,010
+تنتج الطاقة ولكن تساهم في إنتاج الطاقة هي بتساعد كـ
+
+271
+00:20:45,010 --> 00:20:49,010
+Co-factor بتساعد الإنزيم على إنتاج الطاقة
+
+272
+00:20:49,010 --> 00:20:52,630
+بِيكومبليكس فيتامين are often associated with giving
+
+273
+00:20:52,630 --> 00:20:57,770
+a person more energy بتساهم في إنتاج الطاقة فبتزود
+
+274
+00:20:57,770 --> 00:21:01,030
+طاقة this is due to the fact this vitamin each
+
+275
+00:21:01,030 --> 00:21:04,370
+play different role in energy metabolism أنها 
+
+276
+00:21:04,370 --> 00:21:08,570
+بتلعب كيف بتساهم بإعطاء الشخص الطاقة ليس عن طريق
+
+277
+00:21:08,570 --> 00:21:13,840
+إنتاج الطاقة وإنها بتنتجش الطاقة من نفسها، هي
+
+278
+00:21:13,840 --> 00:21:17,920
+نفسها تزوّد الجسم بالطاقة، لأ وإنما إنها تساهم كـ
+
+279
+00:21:17,920 --> 00:21:24,640
+Co-factor كمساعد لإنتاج الطاقة بالرغم من أن هذه
+
+280
+00:21:24,640 --> 00:21:31,460
+الفيتامينات مميزة بالماء، لا تتم تخزينها في الجسم
+
+281
+00:21:31,460 --> 00:21:35,740
+حكينا، بتم إخراج الزائد منها عبر الكلى مع
+
+282
+00:21:35,740 --> 00:21:39,440
+اليورين، تتدخل في الماء، وتتدخل في الماء، وتتم
+
+283
+00:21:39,440 --> 00:21:42,840
+إكسكريتها من الجسم من خلال اليورين، من خلال 
+
+284
+00:21:42,840 --> 00:21:46,600
+اليورين، لذلك، من المهم أن تتناول الطعام مليء في
+
+285
+00:21:46,600 --> 00:21:51,280
+هذه الفيتامينات كل يوم، لكي توفر للجسم ما يحتاجه
+
+286
+00:21:51,760 --> 00:21:55,880
+إذا هي حاجة إن أنا ولا تخزن في الجسم بدّو المي
+
+287
+00:21:55,880 --> 00:21:59,580
+يتم إخراجها مع الـ urine لازم أتناولها كل فترة من
+
+288
+00:21:59,580 --> 00:22:06,260
+يوم لثلاثة أيام عشان أقضي حاجة الجسم من الأمثلة على
+
+289
+00:22:06,260 --> 00:22:09,100
+الـ water-soluble vitamin في عندنا الـ بيكمبليكس
+
+290
+00:22:09,100 --> 00:22:15,340
+فيتامين الـ بيكمبليكس يعتبر كـ coenzyme يعني بيحفظ
+
+291
+00:22:15,340 --> 00:22:20,440
+الـ enzyme found in the same food الـ بيكمبليكس
+
+292
+00:22:20,440 --> 00:22:25,410
+فيتامين هم بيكمبليكس عبارة عن ثمانية كلهم موجودين في
+
+293
+00:22:25,410 --> 00:22:29,490
+نفس الطعام يعني لو قلت لك واحد نوع مثلا في الـ B
+
+294
+00:22:29,490 --> 00:22:32,990
+-complex الثيامين إذا الثيامين موجود في الجزر يبدأ
+
+295
+00:22:32,990 --> 00:22:36,710
+كل الـ B-complex الثمانية كلهم موجودين في الجزر فالـ
+
+296
+00:22:36,710 --> 00:22:40,970
+single deficiency rare أني أفقد واحد منهم هذا شيء
+
+297
+00:22:40,970 --> 00:22:46,580
+نادر Act together in metabolism بتلعب دور في الـ
+
+298
+00:22:46,580 --> 00:22:50,920
+Metabolism في إنتاج الطاقة Metabolic pathway used
+
+299
+00:22:50,920 --> 00:22:54,960
+by protein, carbohydrate and fat حكينا قبل هيك أن
+
+300
+00:22:54,960 --> 00:22:58,180
+مصدر الطاقة الـ carbohydrate ومخزن الطاقة هي
+
+301
+00:22:58,180 --> 00:23:01,980
+الدهون وطاقة الخلايا للبناء هي البروتين يعني هدول
+
+302
+00:23:01,980 --> 00:23:07,550
+مسؤولين عن إنتاج الطاقة للجسم الـ Vitamin بتساهم في
+
+303
+00:23:07,550 --> 00:23:10,030
+إنتاج الطاقة، بتساعد هدول الـ carbohydrate
+
+304
+00:23:10,030 --> 00:23:13,730
+والبروتين والدهن على إنهم يعطوا الطاقة للجسم، لكن
+
+305
+00:23:13,730 --> 00:23:19,110
+هي بنفسها لا تنتج الطاقة مين هما الـ B-complex
+
+306
+00:23:19,110 --> 00:23:22,830
+vitamin؟ فيها ناس صيامين، اللي هو B1, riboflavin
+
+307
+00:23:22,830 --> 00:23:28,830
+B2, niacin B3, pantothenic acid, biotin, pyridoxin
+
+308
+00:23:28,830 --> 00:23:33,570
+B6 و الـ folate و vitamin B12، هدول كلهم vitamin
+
+309
+00:23:33,570 --> 00:23:38,380
+B فهم أكتر من نوع أطلقنا عليهم B Complex Vitamin
+
+310
+00:23:38,380 --> 00:23:45,280
+وكلهم موجودين في نفس نوع الطعام إيش وظائف الـ B
+
+311
+00:23:45,280 --> 00:23:49,720
+Complex؟ أول وظيفة هي Energy Metabolism حكينا أنها 
+
+312
+00:23:49,720 --> 00:23:54,670
+بتساهم في إنتاج الطاقة و هنجسم اللي هيهم مين منهم
+
+313
+00:23:54,670 --> 00:23:57,830
+اللي بتساهم في إنتاج الطاقة و هذا مش مطلوب منها،
+
+314
+00:23:57,830 --> 00:24:02,110
+مطلوب منها نعرف بس الوظائف كعنوان الـ بيك كومبليكس
+
+315
+00:24:02,110 --> 00:24:05,490
+أول وظيفة إلها energy metabolism إنتاج الطاقة
+
+316
+00:24:05,490 --> 00:24:09,910
+تساهم في إنتاج الطاقة red blood cell synthesis
+
+317
+00:24:09,910 --> 00:24:13,970
+إنها بتساهم في إنتاج خلايا الدم الحمراء كريات الدم
+
+318
+00:24:13,970 --> 00:24:18,960
+الحمراء Homocysteine Metabolism اللي هو عملية
+
+319
+00:24:18,960 --> 00:24:23,100
+Metabolism لحاجة نسميها الـ Homocysteine
+
+320
+00:24:26,000 --> 00:24:31,860
+vitamin C اسمه الكيميائي بنطلق عليه اسم ascorbic
+
+321
+00:24:31,860 --> 00:24:36,820
+acid ascorbic acid أو vitamin C synthesizes by most
+
+322
+00:24:36,820 --> 00:24:42,060
+animal بتتم تصنيعه من معظم الحيوانات لكن الإنسان
+
+323
+00:24:42,060 --> 00:24:47,000
+لأ كل الحيوانات بتصنعه ما عدا الإنسان الإنسان غير
+
+324
+00:24:47,000 --> 00:24:51,460
+قادر على تصنيع vitamin C decrease absorption with 
+
+325
+00:24:51,460 --> 00:24:56,220
+high intake أكيد هيقلل الامتصاص إذا أنا عند زيادة 
+
+326
+00:24:56,220 --> 00:24:59,900
+التناولة إذا الجسم حاسس إن هو موجود بكميات كافية 
+
+327
+00:24:59,900 --> 00:25:04,620
+هيقلل الامتصاص الـ excess منه الكميات الزائدة هيصير
+
+328
+00:25:04,620 --> 00:25:10,660
+excretion عملية إخراج لها food source of vitamin C
+
+329
+00:25:10,660 --> 00:25:16,890
+وين موجود؟ موجود في الـ citrus .. citrus fruit اللي
+
+330
+00:25:16,890 --> 00:25:20,890
+هي الفواكه الحمضية موجود في الـ .. في الـ potato، في
+
+331
+00:25:20,890 --> 00:25:24,230
+الـ green pepper، في الفلفل الأخضر، في الزهرة، في
+
+332
+00:25:24,230 --> 00:25:27,470
+الـ broccoli، في الـ strawberry، الفراولة، في الـ
+
+333
+00:25:27,470 --> 00:25:31,350
+romaine lettuce، الخس، في الـ spinach، السبانخ، مش 
+
+334
+00:25:31,350 --> 00:25:36,830
+مطلوب إننا نحفظ الأطعمة function of vitamin C،
+
+335
+00:25:36,830 --> 00:25:41,650
+إيش وظائف vitamin C؟ vitamin C يعتبر زي vitamin E،
+
+336
+00:25:41,650 --> 00:25:46,950
+إن هو reducing agent، يعني antioxidant، vitamin C
+
+337
+00:25:46,950 --> 00:25:53,350
+و E يعتبروا antioxidant reducing agent إيش وظيفته؟
+
+338
+00:25:53,350 --> 00:25:58,170
+بيحفظ الـ iron absorption enhanced يحفظ امتصاص
+
+339
+00:25:58,170 --> 00:26:03,550
+الحديد، يحفظ امتصاص الحديد عشان هيك مثلا لو واحد
+
+340
+00:26:03,550 --> 00:26:07,590
+عنده نقص في الحديد، بيعطوه حبوب حديد، بيحكولي يفضل
+
+341
+00:26:07,590 --> 00:26:11,850
+لو تتناول الحبوب الحديد مع عصير برتقال عشان عصير
+
+342
+00:26:11,850 --> 00:26:16,130
+البرتقال يحتوي على vitamin C و vitamin C بيحفظ
+
+343
+00:26:16,130 --> 00:26:21,280
+الامتصاص Synthesis of collagen كمان هو مسؤول عن 
+
+344
+00:26:21,280 --> 00:26:25,060
+تصنيع الكولاجين اللي موجود في الجلد Immune
+
+345
+00:26:25,060 --> 00:26:29,940
+function اللي وظائف منعية إيش وظيفته المناعية بيحكي
+
+346
+00:26:29,940 --> 00:26:33,160
+اللي doesn't prevent common cold or flu but may 
+
+347
+00:26:33,160 --> 00:26:37,420
+reduce duration of symptom day by day إن هو بيجي لما
+
+348
+00:26:37,420 --> 00:26:40,700
+الواحد يصير معاه influenza بيحكوله اشرب عصير 
+
+349
+00:26:40,700 --> 00:26:46,520
+برتقال أو تناول البرتقال هذا بيقلل اللي هو الفترة
+
+350
+00:26:47,240 --> 00:26:50,940
+vitamin C الموجود في عصير البرتقال بيقلل فترة 
+
+351
+00:26:50,940 --> 00:26:55,760
+أعراض المرض ولا يمنع الـ influenza وعراض الـ burn 
+
+352
+00:26:55,760 --> 00:27:01,280
+وإنما يقلل فترة هذه الأعراض إذا لا يقضي على الفيروس
+
+353
+00:27:01,280 --> 00:27:05,420
+وإنما يقلل الفترة كمان و من ضمنها wound healing
+
+354
+00:27:05,420 --> 00:27:09,280
+اللي هو التئام الجروح يساعد في التئام الجروح
+
+355
+00:27:09,280 --> 00:27:12,840
+easily lost through cooking برضه هو عبارة عن heat
+
+356
+00:27:12,840 --> 00:27:17,880
+sensitive حساس للحرارة فبيفقد مع درجات الحرارة 
+
+357
+00:27:17,880 --> 00:27:22,500
+العالية لإن لو عصير البرتقال سخنتيه خلاص vitamin C
+
+358
+00:27:22,500 --> 00:27:26,400
+بيتكسر بيروح مش هيدّي موجود sensitive to heat
+
+359
+00:27:28,720 --> 00:27:33,140
+vitamin C deficiency إذا صار عندي فقد أو نقص فيه 
+
+360
+00:27:33,140 --> 00:27:38,620
+vitamin C أشهر مرض بينتج عن نقص vitamin C هو مرض
+
+361
+00:27:38,620 --> 00:27:42,860
+الـ scurvy الاسقربوت مرض scurvy disease اللي هو
+
+362
+00:27:42,860 --> 00:27:47,620
+الاسقربوت disease characterized by weakness, small
+
+363
+00:27:47,620 --> 00:27:52,960
+hemorrhage throughout the body that cause gum and
+
+364
+00:27:52,960 --> 00:27:58,030
+skin to bleed هو مرض بيكون عبارة عن خصائصه أنه
+
+365
+00:27:58,030 --> 00:28:03,690
+بيكون ضعف عام في الجسم، بيكون فيه نزيف في مسامات
+
+366
+00:28:03,690 --> 00:28:08,390
+الجلد، مسامات الجلد بتفرز دم، ممكن نزيف في اللثة و
+
+367
+00:28:08,390 --> 00:28:14,580
+سقوط الأسنان في عندنا صورة تحت مثلا Sclerotic
+
+368
+00:28:14,580 --> 00:28:18,580
+Rosary، هاي من المرض إنه بيكون عندنا بروز للقفص 
+
+369
+00:28:18,580 --> 00:28:22,840
+الصدري، القفص الصدري بارز للأمام، في عندنا بيكون
+
+370
+00:28:22,840 --> 00:28:28,460
+نزيف في اللثة، تساقط للأسنان، في عندنا Volicular
+
+371
+00:28:28,460 --> 00:28:32,580
+Hemorrhage، اللي هو نزيف في مسامات الجلد، تحت
+
+372
+00:28:32,580 --> 00:28:38,820
+الجلد بيلاقي بقع زي نزيف Vitamin C excess لو كان
+
+373
+00:28:38,820 --> 00:28:43,940
+في عندنا كميات زائدة من vitamin C اللي هو زيادة في 
+
+374
+00:28:43,940 --> 00:28:47,940
+حاجة الجسم وهي زيادة أكيد rare لأن هو water
+
+375
+00:28:47,940 --> 00:28:5
\ No newline at end of file
diff --git a/PL9fwy3NUQKwZ1Q7zOg1ObgufPMjRI3WY1/ejpJCjHaqTg_raw.json b/PL9fwy3NUQKwZ1Q7zOg1ObgufPMjRI3WY1/ejpJCjHaqTg_raw.json
new file mode 100644
index 0000000000000000000000000000000000000000..62a328d54d11e711dc5a9640e09445294767d76d
--- /dev/null
+++ b/PL9fwy3NUQKwZ1Q7zOg1ObgufPMjRI3WY1/ejpJCjHaqTg_raw.json
@@ -0,0 +1 @@
+{"segments": [{"id": 1, "seek": 811, "start": 0.0, "end": 8.12, "text": "بنحكي في موضوع جديد هو موضوع الفيتامينات، أتحدث عن الفيتامينات و عن أقسام الفيتامينات", "tokens": [3555, 1863, 5016, 4117, 1829, 8978, 3714, 2407, 11242, 45367, 10874, 16254, 3215, 31439, 3714, 2407, 11242, 45367, 27188, 36081, 10943, 1829, 8315, 2655, 12399, 5551, 2655, 24401, 12984, 18871, 27188, 36081, 10943, 1829, 8315, 2655, 4032, 18871, 5551, 4587, 3794, 10943, 27188, 36081, 10943, 1829, 8315, 2655], "avg_logprob": -0.11631058916753652, "compression_ratio": 1.6881720430107527, "no_speech_prob": 4.76837158203125e-07, "words": [{"start": 0.0, "end": 0.98, "word": "بنحكي", "probability": 0.868798828125}, {"start": 0.98, "end": 1.12, "word": " في", "probability": 0.923828125}, {"start": 1.12, "end": 1.54, "word": " موضوع", "probability": 0.9971923828125}, {"start": 1.54, "end": 2.04, "word": " جديد", "probability": 0.9920247395833334}, {"start": 2.04, "end": 2.2, "word": " هو", "probability": 0.7548828125}, 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"probability": 0.9390869140625}, {"start": 1726.18, "end": 1726.4, "word": " أكيد", "probability": 0.7082112630208334}, {"start": 1726.4, "end": 1726.74, "word": " rare", "probability": 0.69580078125}, {"start": 1726.74, "end": 1727.14, "word": " لأن", "probability": 0.779296875}, {"start": 1727.14, "end": 1727.54, "word": " هو", "probability": 0.478759765625}, {"start": 1727.54, "end": 1727.94, "word": " water", "probability": 0.88525390625}, {"start": 1727.94, "end": 1728.4, "word": " soluble", "probability": 0.802734375}, {"start": 1728.4, "end": 1728.8, "word": " vitamin", "probability": 0.8955078125}, {"start": 1728.8, "end": 1729.26, "word": " بيصيرله", "probability": 0.8294921875}, {"start": 1729.26, "end": 1730.0, "word": " excretion", "probability": 0.9208984375}, {"start": 1730.0, "end": 1730.48, "word": " بيصيرله", "probability": 0.94453125}, {"start": 1730.48, "end": 1730.92, "word": " إخراج", "probability": 0.89013671875}, {"start": 1730.92, "end": 1731.74, "word": " بس", "probability": 0.883056640625}, {"start": 1731.74, "end": 1732.04, "word": " إذا", "probability": 0.959716796875}, {"start": 1732.04, "end": 1732.34, "word": " كان", "probability": 0.99169921875}, {"start": 1732.34, "end": 1732.98, "word": " بكميات", "probability": 0.824951171875}, {"start": 1732.98, "end": 1733.62, "word": " زائدة", "probability": 0.9928385416666666}, {"start": 1733.62, "end": 1734.04, "word": " بسبب", "probability": 0.990234375}, {"start": 1734.04, "end": 1734.44, "word": " خلال", "probability": 0.9236653645833334}, {"start": 1734.44, "end": 1734.94, "word": " ماء", "probability": 0.804443359375}, {"start": 1734.94, "end": 1736.32, "word": " بيصير", "probability": 0.9013671875}, {"start": 1736.32, "end": 1736.66, "word": " عندي", "probability": 0.767822265625}, {"start": 1736.66, "end": 1736.92, "word": " مرض", "probability": 0.981689453125}, {"start": 1736.92, "end": 1738.14, "word": " hemochromatosis", "probability": 0.781298828125}], "temperature": 1.0}, {"id": 79, "seek": 176471, "start": 1739.39, "end": 1764.71, "text": "Hemochromatosis معناها زيادة نسبة الحديد في الجسم زيادة تخزين الحديد Iron overload لأنه حكينا أن Vitamin C بيحفظ امتصاص الحديد فإذا زاد Vitamin C هيمتص كمية من الحديد الزيادة هيزود كمية امتصاص الحديد وسبب مرض اسمه Hemochromatosis اللي هو Iron overload زيادة الحديد", "tokens": [39, 443, 8997, 4397, 267, 8211, 20449, 8315, 11296, 30767, 1829, 18513, 3660, 8717, 35457, 3660, 21542, 16254, 3215, 8978, 25724, 38251, 30767, 1829, 18513, 3660, 6055, 9778, 11622, 9957, 21542, 16254, 3215, 13720, 28777, 5296, 33456, 3224, 11331, 4117, 1829, 8315, 14739, 33515, 383, 4724, 1829, 5016, 5172, 19913, 1975, 2304, 2655, 9381, 33546, 21542, 16254, 3215, 6156, 28814, 15730, 30767, 18513, 33515, 383, 39896, 2304, 2655, 9381, 9122, 2304, 10632, 9154, 21542, 16254, 3215, 6024, 110, 1829, 18513, 3660, 39896, 11622, 23328, 9122, 2304, 10632, 1975, 2304, 2655, 9381, 33546, 21542, 16254, 3215, 46952, 3555, 3555, 3714, 43042, 24525, 2304, 3224, 18568, 8997, 4397, 267, 8211, 13672, 1829, 31439, 13720, 28777, 30767, 1829, 18513, 3660, 21542, 16254, 3215], "avg_logprob": -0.10653409090909091, "compression_ratio": 1.9259259259259258, "no_speech_prob": 5.364418029785156e-07, "words": [{"start": 1739.39, "end": 1740.43, "word": "Hemochromatosis", "probability": 0.8249104817708334}, {"start": 1740.43, "end": 1741.11, "word": " معناها", "probability": 0.9625651041666666}, {"start": 1741.11, "end": 1742.19, "word": " زيادة", "probability": 0.9874267578125}, {"start": 1742.19, "end": 1742.71, "word": " نسبة", "probability": 0.99365234375}, {"start": 1742.71, "end": 1743.27, "word": " الحديد", "probability": 0.9786783854166666}, {"start": 1743.27, "end": 1743.41, "word": " في", "probability": 0.97021484375}, {"start": 1743.41, "end": 1743.85, "word": " الجسم", "probability": 0.988525390625}, {"start": 1743.85, "end": 1744.99, "word": " زيادة", "probability": 0.9173583984375}, {"start": 1744.99, "end": 1745.47, "word": " تخزين", "probability": 0.9891357421875}, {"start": 1745.47, "end": 1745.93, "word": " الحديد", "probability": 0.9840494791666666}, {"start": 1745.93, "end": 1746.43, "word": " Iron", "probability": 0.55322265625}, {"start": 1746.43, "end": 1747.15, "word": " overload", "probability": 0.73046875}, {"start": 1747.15, "end": 1748.13, "word": " لأنه", "probability": 0.8974609375}, {"start": 1748.13, "end": 1748.53, "word": " حكينا", "probability": 0.759033203125}, {"start": 1748.53, "end": 1748.65, "word": " أن", "probability": 0.56982421875}, {"start": 1748.65, "end": 1749.09, "word": " Vitamin", "probability": 0.1953125}, {"start": 1749.09, "end": 1749.45, "word": " C", "probability": 0.8994140625}, {"start": 1749.45, "end": 1750.05, "word": " بيحفظ", "probability": 0.91103515625}, {"start": 1750.05, "end": 1750.83, "word": " امتصاص", "probability": 0.901953125}, {"start": 1750.83, "end": 1751.53, "word": " الحديد", "probability": 0.9869791666666666}, {"start": 1751.53, "end": 1752.25, "word": " فإذا", "probability": 0.9611002604166666}, {"start": 1752.25, "end": 1752.61, "word": " زاد", "probability": 0.979736328125}, {"start": 1752.61, "end": 1753.03, "word": " Vitamin", "probability": 0.87060546875}, {"start": 1753.03, "end": 1753.43, "word": " C", "probability": 0.9912109375}, {"start": 1753.43, "end": 1754.27, "word": " هيمتص", "probability": 0.8121337890625}, {"start": 1754.27, "end": 1754.83, "word": " كمية", "probability": 0.9873046875}, {"start": 1754.83, "end": 1755.11, "word": " من", "probability": 0.92822265625}, {"start": 1755.11, "end": 1755.69, "word": " الحديد", "probability": 0.9876302083333334}, {"start": 1755.69, "end": 1756.21, "word": " الزيادة", "probability": 0.9171875}, {"start": 1756.21, "end": 1756.71, "word": " هيزود", "probability": 0.8592122395833334}, {"start": 1756.71, "end": 1757.09, "word": " كمية", "probability": 0.9933268229166666}, {"start": 1757.09, "end": 1757.51, "word": " امتصاص", "probability": 0.9609375}, {"start": 1757.51, "end": 1757.99, "word": " الحديد", "probability": 0.9884440104166666}, {"start": 1757.99, "end": 1758.73, "word": " وسبب", "probability": 0.7639973958333334}, {"start": 1758.73, "end": 1759.03, "word": " مرض", "probability": 0.99072265625}, {"start": 1759.03, "end": 1759.55, "word": " اسمه", "probability": 0.9703776041666666}, {"start": 1759.55, "end": 1760.91, "word": " Hemochromatosis", "probability": 0.94521484375}, {"start": 1760.91, "end": 1761.11, "word": " اللي", "probability": 0.90576171875}, {"start": 1761.11, "end": 1761.33, "word": " هو", "probability": 0.98291015625}, {"start": 1761.33, "end": 1761.93, "word": " Iron", "probability": 0.87353515625}, {"start": 1761.93, "end": 1762.77, "word": " overload", "probability": 0.79150390625}, {"start": 1762.77, "end": 1764.05, "word": " زيادة", "probability": 0.9598388671875}, {"start": 1764.05, "end": 1764.71, "word": " الحديد", "probability": 0.9903971354166666}], "temperature": 1.0}, {"id": 80, "seek": 178208, "start": 1766.0, "end": 1782.08, "text": "أجزالات كيدنستون اللي هي حصاوي في الكيلة من نوع أجزالات أجزالات كيدنستون اللي هي حصاوي من نوع أجزالات ايه ال tooth enamel؟ اللي هو تآكل للأسنان، بيصير عندي تآكل للأسنان", "tokens": [10721, 7435, 11622, 6027, 9307, 9122, 25708, 1863, 14851, 11536, 13672, 1829, 39896, 11331, 9381, 995, 45865, 8978, 33251, 26895, 3660, 9154, 8717, 45367, 5551, 7435, 11622, 6027, 9307, 5551, 7435, 11622, 6027, 9307, 9122, 25708, 1863, 14851, 11536, 13672, 1829, 39896, 11331, 9381, 995, 45865, 9154, 8717, 45367, 5551, 7435, 11622, 6027, 9307, 1975, 1829, 3224, 2423, 11680, 465, 16103, 22807, 13672, 1829, 31439, 6055, 148, 95, 28820, 24976, 10721, 3794, 1863, 7649, 12399, 4724, 1829, 9381, 13546, 18871, 16254, 6055, 148, 95, 28820, 24976, 10721, 3794, 1863, 7649], "avg_logprob": -0.1926510920236399, "compression_ratio": 2.0273972602739727, "no_speech_prob": 0.0, "words": [{"start": 1766.0, "end": 1766.7, "word": "أجزالات", "probability": 0.694921875}, {"start": 1766.7, "end": 1767.6, "word": " كيدنستون", "probability": 0.741845703125}, {"start": 1767.6, "end": 1768.1, "word": " اللي", "probability": 0.7705078125}, {"start": 1768.1, "end": 1768.24, "word": " هي", "probability": 0.888671875}, {"start": 1768.24, "end": 1768.9, "word": " حصاوي", "probability": 0.9556884765625}, {"start": 1768.9, "end": 1769.18, "word": " في", "probability": 0.97119140625}, {"start": 1769.18, "end": 1769.9, "word": " الكيلة", "probability": 0.69189453125}, {"start": 1769.9, "end": 1770.08, "word": " من", "probability": 0.986328125}, {"start": 1770.08, "end": 1770.4, "word": " نوع", "probability": 0.97998046875}, {"start": 1770.4, "end": 1771.16, "word": " أجزالات", "probability": 0.9423828125}, {"start": 1771.16, "end": 1772.14, "word": " أجزالات", "probability": 0.88017578125}, {"start": 1772.14, "end": 1772.88, "word": " كيدنستون", "probability": 0.986328125}, {"start": 1772.88, "end": 1773.08, "word": " اللي", "probability": 0.95361328125}, {"start": 1773.08, "end": 1773.18, "word": " هي", "probability": 0.9501953125}, {"start": 1773.18, "end": 1773.74, "word": " حصاوي", "probability": 0.9462890625}, {"start": 1773.74, "end": 1773.86, "word": " من", "probability": 0.446533203125}, {"start": 1773.86, "end": 1773.98, "word": " نوع", "probability": 0.98828125}, {"start": 1773.98, "end": 1774.78, "word": " أجزالات", "probability": 0.7515625}, {"start": 1774.78, "end": 1775.42, "word": " ايه", "probability": 0.5928548177083334}, {"start": 1775.42, "end": 1775.6, "word": " ال", "probability": 0.7705078125}, {"start": 1775.6, "end": 1775.9, "word": " tooth", "probability": 0.5439453125}, {"start": 1775.9, "end": 1776.74, "word": " enamel؟", "probability": 0.6953938802083334}, {"start": 1776.74, "end": 1776.86, "word": " اللي", "probability": 0.9150390625}, {"start": 1776.86, "end": 1777.06, "word": " هو", "probability": 0.921875}, {"start": 1777.06, "end": 1777.8, "word": " تآكل", "probability": 0.79595947265625}, {"start": 1777.8, "end": 1778.74, "word": " للأسنان،", "probability": 0.8544921875}, {"start": 1778.74, "end": 1779.1, "word": " بيصير", "probability": 0.960693359375}, {"start": 1779.1, "end": 1779.54, "word": " عندي", "probability": 0.882080078125}, {"start": 1779.54, "end": 1780.72, "word": " تآكل", "probability": 0.96923828125}, {"start": 1780.72, "end": 1782.08, "word": " للأسنان", "probability": 0.95224609375}], "temperature": 1.0}], "language": "ar", "language_probability": 1.0, "duration": 1783.4783125, "duration_after_vad": 1731.9347187499966}
\ No newline at end of file
diff --git a/PL9fwy3NUQKwZ1Q7zOg1ObgufPMjRI3WY1/ejpJCjHaqTg_raw.srt b/PL9fwy3NUQKwZ1Q7zOg1ObgufPMjRI3WY1/ejpJCjHaqTg_raw.srt
new file mode 100644
index 0000000000000000000000000000000000000000..614bc907f46ed48aac57e8356088f568af0a7269
--- /dev/null
+++ b/PL9fwy3NUQKwZ1Q7zOg1ObgufPMjRI3WY1/ejpJCjHaqTg_raw.srt
@@ -0,0 +1,1544 @@
+1
+00:00:00,000 --> 00:00:04,920
+بنحكي في موضوع جديد هو موضوع الفيتامينات، أتحدث عن
+
+2
+00:00:04,920 --> 00:00:10,570
+الفيتامينات و عن أقسام الفيتاميناتأولش نحكي عن
+
+3
+00:00:10,570 --> 00:00:15,190
+خصائص ال vitamins characteristics of vitamins أولش
+
+4
+00:00:15,190 --> 00:00:19,930
+ال vitamins هي عبارة عن micro nutrients هي مغذيات
+
+5
+00:00:19,930 --> 00:00:24,530
+صغيرة أول خاصية انها تعتبر micro nutrients مغذيات
+
+6
+00:00:24,530 --> 00:00:29,530
+صغيرة ليش بيعتبرها micro لأن very small amount are
+
+7
+00:00:29,530 --> 00:00:34,620
+needed by the body الجسم بيحتاجها بكميات قليلةvery
+
+8
+00:00:34,620 --> 00:00:38,820
+small amount are contained in food و الأطعمة تحتوى
+
+9
+00:00:38,820 --> 00:00:43,380
+على الفيتامينات بكميات قليلة، إذن هي مغاديات صغيرة
+
+10
+00:00:43,380 --> 00:00:47,840
+لأنه بيحتاجها الجسم بكميات قليلة و كمان موجودة في
+
+11
+00:00:47,840 --> 00:00:52,360
+الأطعمة بكميات قليلةبعد هيك ال vitamins are
+
+12
+00:00:52,360 --> 00:00:57,160
+essential الفيتامينات ضرورية ومهمة للجسم لأنها
+
+13
+00:00:57,160 --> 00:01:02,100
+بتلعب دور مهم في الجسم play important role in the
+
+14
+00:01:02,100 --> 00:01:06,380
+body most vitamins are obtained from the food we
+
+15
+00:01:06,380 --> 00:01:10,680
+eat من ورا الجسم بياخد الفيتامينات معظم
+
+16
+00:01:10,680 --> 00:01:15,260
+الفيتامينات بياخدها من الطعام الطعام اللي بيتناوره
+
+17
+00:01:15,260 --> 00:01:20,790
+بيحتوي على الفيتاميناتفي بعض الفيتامينات بتصنحها
+
+18
+00:01:20,790 --> 00:01:25,870
+بكتيريا موجودة في الأمعاء زي ال normal flora بتصنع
+
+19
+00:01:25,870 --> 00:01:30,680
+vitamin Kبعض الفيتامينات بتصنعها بكتيريا موجودة
+
+20
+00:01:30,680 --> 00:01:35,260
+فيه لما و أما معظم الفيتامينات بناخدها من الطعام
+
+21
+00:01:35,260 --> 00:01:38,600
+there is no perfect food that contain all the
+
+22
+00:01:38,600 --> 00:01:43,000
+vitamins in the right amount فش طعام واحد مثلا
+
+23
+00:01:43,000 --> 00:01:47,820
+أقولك الطماطم تحتوي على كل أنواع الفيتامينات لأ
+
+24
+00:01:47,820 --> 00:01:51,880
+الفيتامين منتشر في أطعم مختلفة لكن فش طعام واحد
+
+25
+00:01:51,880 --> 00:01:53,980
+يحتوي كل أنواع الفيتامين
+
+26
+00:01:58,030 --> 00:02:02,990
+هي الفيتامينات لا تحتوي سعرات حرارية، not contain
+
+27
+00:02:02,990 --> 00:02:06,470
+kilo calories، لا تحتوي سعرات حرارية وبالتالي هي
+
+28
+00:02:06,470 --> 00:02:12,270
+لا تعطي طاقة ولا تنتج الطاقة vitamin are
+
+29
+00:02:12,270 --> 00:02:15,270
+classified according to how soluble they are in
+
+30
+00:02:15,270 --> 00:02:19,530
+fat or water الفيتامينات مقسمة حسب دوبانيتها، في
+
+31
+00:02:19,530 --> 00:02:24,980
+منها تدوب في الدهون وفي منها تدوب في الماءعنا هاد
+
+32
+00:02:24,980 --> 00:02:28,960
+الجدول بيفرق بين ال vitamins اللي هم على قسمين
+
+33
+00:02:28,960 --> 00:02:32,440
+العمودي الأول اللي هى ال water soluble vitamins
+
+34
+00:02:32,440 --> 00:02:37,140
+وفيه عنا fat soluble vitamins أنثى على water
+
+35
+00:02:37,140 --> 00:02:41,860
+soluble vitamins عنا vitamin B وC أما ال fat
+
+36
+00:02:41,860 --> 00:02:48,160
+soluble vitamins عنا vitamin A DE و K مجموعين في
+
+37
+00:02:48,160 --> 00:02:53,920
+كلمة أكيد أو أديك، لذا الفات سوليوبين في عندنا و
+
+38
+00:02:53,920 --> 00:02:57,160
+في عندنا water soluble vitamin ال water soluble هي
+
+39
+00:02:57,160 --> 00:03:03,550
+ال B و ال C، الفات سوليوبين هم A و D و E و Kطيب
+
+40
+00:03:03,550 --> 00:03:06,570
+نيجي نفرق بينهم أول إشي من ناحية ال absorption
+
+41
+00:03:06,570 --> 00:03:11,370
+مدام هو water soluble vitamins ال water soluble
+
+42
+00:03:11,370 --> 00:03:15,690
+vitamins يعني بتدوب في المي والجسم على طول أغلب
+
+43
+00:03:15,690 --> 00:03:19,730
+مكوناته مي فهي على طول directly into the blood بتم
+
+44
+00:03:19,730 --> 00:03:24,970
+إمتصاصها مباشرة عبر الدم أما الفات سوليوبل لازم
+
+45
+00:03:24,970 --> 00:03:29,510
+بأول تروح على ال lymph و بعدين تيجي على الدم عشان
+
+46
+00:03:29,510 --> 00:03:35,190
+يتم تحميلوهي تنقلها، إذاً الامتصاص ال water
+
+47
+00:03:35,190 --> 00:03:38,590
+soluble يتم امتصاصها مباشرة في الدم أما الفاة
+
+48
+00:03:38,590 --> 00:03:42,370
+soluble vitamins لازم تروح على اللنف وبعدين يتم
+
+49
+00:03:42,370 --> 00:03:48,380
+نقلها للبلادطيب transport كيف بدها تتنقل هاي ال
+
+50
+00:03:48,380 --> 00:03:52,140
+water soluble مدى ما خصائصها زي خصائص الجسم فهي
+
+51
+00:03:52,140 --> 00:03:56,260
+travel freely بتنتقل بحرية، بتمشي بحرية من غير أي
+
+52
+00:03:56,260 --> 00:04:00,200
+معوقة أما ال fat soluble vitamins لأن خصائصها
+
+53
+00:04:00,200 --> 00:04:05,270
+بتغير عن الجسمفبتحتاج إلى نواقل require carrier
+
+54
+00:04:05,270 --> 00:04:09,030
+proteins بتحتاج إلى نواقل عشان تنجولها وعشان تمشي
+
+55
+00:04:09,030 --> 00:04:13,590
+في داخل الجسم ال storage التخزين هل بيتم تخزينها
+
+56
+00:04:13,590 --> 00:04:18,430
+ولا لا ال water soluble vitamins بتمش تخزينها بدل
+
+57
+00:04:18,430 --> 00:04:22,150
+هذا كل اللي مكتوب circulated freely لنكتب not
+
+58
+00:04:22,150 --> 00:04:27,150
+stored لاتخزن ما بيتم تخزينها أما ال fat soluble
+
+59
+00:04:27,150 --> 00:04:31,240
+vitamin بتم تخزينها it is storedwhen بتخزن في
+
+60
+00:04:31,240 --> 00:04:36,400
+الأنسجة الدهنية، في الخلايا الدهنية حسنا طبيعة
+
+61
+00:04:36,400 --> 00:04:40,480
+خلايا الجسم دهنية فهتتخزن ال fat soluble vitamins
+
+62
+00:04:40,480 --> 00:04:45,060
+اللي بتشبهها، إذا ال water لا تخزن أما الفات تخزن
+
+63
+00:04:45,060 --> 00:04:50,390
+excretion هجيت الإخراجمدام ال water لا تخزن في
+
+64
+00:04:50,390 --> 00:04:55,710
+الجسم فاللي هيكون زائد عن حاجة الجسم هيتم إخراجه
+
+65
+00:04:55,710 --> 00:05:00,530
+ال kidney هتعمل ال detection هتعمله فلترة وتعمله
+
+66
+00:05:00,530 --> 00:05:04,130
+remove excess in urine وهتعمله إخراج مع ال urine
+
+67
+00:05:04,130 --> 00:05:08,330
+ال excretion معناه الإخراج ال water soluble
+
+68
+00:05:08,330 --> 00:05:12,340
+vitamins مدام مافيش تخزين لهامش هيتخزن في الجسم
+
+69
+00:05:12,340 --> 00:05:16,960
+فالكمية الزائدة عن حاجة الجسم هيتم إخراجها مع
+
+70
+00:05:16,960 --> 00:05:21,660
+اليورو أما الفات سوليوبل vitamins مدامها بتخزن إذا
+
+71
+00:05:21,660 --> 00:05:26,620
+الكمية الزائدة عن حاجة الجسم بتم تخزينها في
+
+72
+00:05:26,620 --> 00:05:30,880
+الأنسجة و الخلايا الدهنية إذا في ال execution في
+
+73
+00:05:30,880 --> 00:05:34,360
+الفوتر سوليوبل بتم execution أما في الفات no
+
+74
+00:05:34,360 --> 00:05:40,650
+executionال toxicity اللي هو السمية أنه يوصل لحد
+
+75
+00:05:40,650 --> 00:05:44,490
+السمية كمية الفيتامين في الجسم صارت زيادة كمية
+
+76
+00:05:44,490 --> 00:05:49,710
+كبيرة ووصلت لحد السمية هلجت ال water soluble
+
+77
+00:05:49,710 --> 00:05:52,470
+possible to reach toxic level؟
+
+78
+00:05:54,740 --> 00:05:59,740
+الـ toxicity حكينا السمية أن يوصل لحد السمية ال
+
+79
+00:05:59,740 --> 00:06:03,000
+water soluble بتتم إخراجها أصلا خارج الجسم فممكن
+
+80
+00:06:03,000 --> 00:06:08,720
+توصل لحد السمية ولكن يعني احتمال قليل و بيحكي إن
+
+81
+00:06:08,720 --> 00:06:12,440
+هذه ال toxicity بتيجي من consumed from supplement
+
+82
+00:06:12,440 --> 00:06:17,840
+لما ياخد supplement مدعمات أو أقراص vitamins ممكن
+
+83
+00:06:17,840 --> 00:06:21,580
+هنا نوصل لحد السمية في ال fat soluble vitamins
+
+84
+00:06:21,910 --> 00:06:26,890
+السمية عالية احتمال انه نوصل لحد السمية likely ليش
+
+85
+00:06:26,890 --> 00:06:31,290
+لأنه اصلا بيخزن في الجسمطب ال requirements اللي هو
+
+86
+00:06:31,290 --> 00:06:34,750
+حاجة الجسم إلها من جداش لجداش الفترة أنا لازم
+
+87
+00:06:34,750 --> 00:06:38,630
+أتناول هذه الفيتامينة ال water soluble vitamins
+
+88
+00:06:38,630 --> 00:06:43,450
+بنحتاجه frequent dose يعني من كل يوم لتلت أيام بدي
+
+89
+00:06:43,450 --> 00:06:47,710
+منه لأنه بيتم إخراجه لكن ال fat soluble لأنه بيتم
+
+90
+00:06:47,710 --> 00:06:52,770
+تخزينه في داخل الجسم ففترات متباعدة خلال periodic
+
+91
+00:06:52,770 --> 00:06:58,240
+dose ممكن من أسبوع لشهراحتاج ال fat soluble
+
+92
+00:06:58,240 --> 00:07:01,820
+vitamin اذا هنا عندنا نوعين من انواع ال vitamins
+
+93
+00:07:01,820 --> 00:07:06,220
+water soluble و ال fat soluble vitamin خدنا مثال
+
+94
+00:07:06,220 --> 00:07:09,760
+على هاي و مثال على هاي و مقارنة بينهم فالجيت هنفصل
+
+95
+00:07:09,760 --> 00:07:14,040
+كل واحدة اول اش ان نحكي عن ال fat soluble vitamin
+
+96
+00:07:14,040 --> 00:07:19,220
+اللي حكينا هم اربعة مجموعين في كلمة اكيد او اديك
+
+97
+00:07:20,670 --> 00:07:23,910
+الخصائص العامة لل fats, soluble vitamins هذا ملخص
+
+98
+00:07:23,910 --> 00:07:26,550
+الجدول اللي خاص في ال fats, soluble vitamins
+
+99
+00:07:26,550 --> 00:07:30,490
+الأطعمة اللي بتكون موجودة فيها الفات و ال soluble
+
+100
+00:07:30,490 --> 00:07:33,290
+vitamins بتكون موجودة في الأطعمة الدهنية و في
+
+101
+00:07:33,290 --> 00:07:38,050
+الزيت in fats and oil require piles for absorption
+
+102
+00:07:38,050 --> 00:07:42,490
+بتحتاج .. لازم نحتاج حاجة عشان تساعد على امتصاصها
+
+103
+00:07:42,490 --> 00:07:47,450
+حكينا فبتحتاج ال piles للامتصاص إذا فاكرين حكينا
+
+104
+00:07:47,450 --> 00:07:51,350
+في معمل ال cholesterolإنه ال bio اللي بتشتغل ك
+
+105
+00:07:51,350 --> 00:07:56,530
+soap كصابونة بتعمل على امتصاص الفات كيف بده ينهضم
+
+106
+00:07:56,530 --> 00:08:01,380
+في الجسم و يتم امتصاصه عبر ال bioEnter the lymph
+
+107
+00:08:01,380 --> 00:08:05,220
+in the blood تدخل الليم في العقد وبعدين بتروح على
+
+108
+00:08:05,220 --> 00:08:09,980
+الدم وبتم تخزينها في الفاتي تشوف الخلايا الدهنية
+
+109
+00:08:09,980 --> 00:08:15,120
+need in small amount وبنحتاجها بكميات قليلة ممكن
+
+110
+00:08:15,120 --> 00:08:20,200
+نوصل لحد السمية لأنه فش إلها إخراج not readily
+
+111
+00:08:20,200 --> 00:08:22,460
+executed ما بتم إخراجها
+
+112
+00:08:25,100 --> 00:08:28,700
+طب أول نوع من أنواع الفات سوليوبيل فيتامين فيتامين
+
+113
+00:08:28,700 --> 00:08:34,700
+A فيتامين A في منه تلت أشكال تلت أشكال من فيتامين
+
+114
+00:08:34,700 --> 00:08:39,520
+A موجود في الجسم على تلت أشكال ريتينول ريتينال
+
+115
+00:08:39,520 --> 00:08:45,060
+ريتينوك أسن إذا الفات ال فيتامين A موجود في الجسم
+
+116
+00:08:45,060 --> 00:08:51,210
+على تلت أشكال ريتينول ريتينالretinoic acid كلهم مع
+
+117
+00:08:51,210 --> 00:08:55,390
+بعض collectively known as retinoid كلهم مع بعض
+
+118
+00:08:55,390 --> 00:09:00,170
+بنسميهم retinoid ال precursor اللي هي الوحدة
+
+119
+00:09:00,170 --> 00:09:03,910
+البنائية في تكوين vitamin A في تكوين ال retinoid
+
+120
+00:09:03,910 --> 00:09:09,830
+الوحدة الأساسية والبنائية هو ال beta carotene ال
+
+121
+00:09:09,830 --> 00:09:15,330
+beta carotene هو الوحدة البنائية لتكوين vitamin A
+
+122
+00:09:17,900 --> 00:09:21,400
+derived from plant food من وين بدى احصل على هذا ال
+
+123
+00:09:21,400 --> 00:09:25,040
+beta carotene عشان يكون لفيتامين a يكون لريتينول
+
+124
+00:09:25,040 --> 00:09:29,500
+ريتينويد بحصل عليه من ال plant food من الأطعمة
+
+125
+00:09:29,500 --> 00:09:34,180
+النباتية وكمان can split and form retinol in
+
+126
+00:09:34,180 --> 00:09:38,500
+intestine and liver وبتم تصنيع في ال liver و ال
+
+127
+00:09:38,500 --> 00:09:43,240
+intestineالبيتا كاروتين one الأطعمة بنلاقيها في
+
+128
+00:09:43,240 --> 00:09:48,060
+الخضروات الورقية الخضراء بنلاقيها في الخضروات اللي
+
+129
+00:09:48,060 --> 00:09:53,140
+نونها برتقالي وفي الفواكه اللي هو البرتقال ال
+
+130
+00:09:53,140 --> 00:09:57,580
+orange fruit vitamin
+
+131
+00:09:57,580 --> 00:10:01,860
+A function إيش وظيفة vitamin A أهم وظيفة لvitamin
+
+132
+00:10:01,860 --> 00:10:05,520
+A ال vision اللي هو في النظر في الرؤية يستخدم في
+
+133
+00:10:05,520 --> 00:10:10,210
+الرؤية maintain epithelial tissue and skinبحافظ
+
+134
+00:10:10,210 --> 00:10:14,810
+على الأنسجة و الخلايا support reproduction and
+
+135
+00:10:14,810 --> 00:10:19,830
+growth بيدعم النمو و البلوغ بساعد في النمو و
+
+136
+00:10:19,830 --> 00:10:24,890
+البلوغ immune system الوظائف في جهاز المناعة bone
+
+137
+00:10:24,890 --> 00:10:31,270
+development الوظائف في نمو و تطور العظم
+
+138
+00:10:33,520 --> 00:10:37,340
+هلجيت vitamin A deficiency اللي هي النقص هلجيت هل
+
+139
+00:10:37,340 --> 00:10:40,640
+في احتمال ايش احتماليته انه يتم نقص vitamin A في
+
+140
+00:10:40,640 --> 00:10:44,680
+الجسم الاحتمال انه يتم نقص vitamin A هو احتمال
+
+141
+00:10:44,680 --> 00:10:48,680
+ضعيف لانه حكينا ان vitamin A من الفات سوليو
+
+142
+00:10:48,680 --> 00:10:52,380
+بالفيتامين و الفات سوليو بالفيتامين بتتم تخزينها
+
+143
+00:10:52,380 --> 00:10:56,200
+في الخلايا الدهنية في الجسم ما بتتم اخراجها
+
+144
+00:10:56,200 --> 00:11:00,740
+فاحتمال النقص احتمال ضعيف لانه بتم تخزينه في الجسم
+
+145
+00:11:00,740 --> 00:11:05,580
+فهو rare ال deficiency rareلكن إذا صار deficiency،
+
+146
+00:11:05,580 --> 00:11:09,860
+إيش بيسبب أمراض؟ بسبب infectious disease، أمراض
+
+147
+00:11:09,860 --> 00:11:14,460
+معضية، زي الـ pneumonia اللي هي الالتهاب الرئوي،
+
+148
+00:11:14,460 --> 00:11:20,510
+measles حصبة، ودياريا shكمان ممكن يسبب
+
+149
+00:11:20,510 --> 00:11:24,970
+keratinization لإنه حكينا إنه من وضع ال vitamin A
+
+150
+00:11:24,970 --> 00:11:29,370
+إنه بيحافظ على الأنسجة و الخلايا الجلد فبيصير إذا
+
+151
+00:11:29,370 --> 00:11:34,130
+نقص هيصير keratinization اللي هو تأثير على
+
+152
+00:11:34,500 --> 00:11:38,400
+keratinization يعني هيصير تأثير على الجلد بيصير
+
+153
+00:11:38,400 --> 00:11:42,900
+تراكم للكيراتين تحت الجلد تراكم للكيراتين تحت
+
+154
+00:11:42,900 --> 00:11:47,140
+الجلد فبيصير الجلد عبارة عن ال dry جاف و rough
+
+155
+00:11:47,140 --> 00:11:51,080
+بيكون خشن و بيكون scaly جلد باهت لذا ال
+
+156
+00:11:51,080 --> 00:11:54,560
+keratinization تراكم الكيراتين تحت الجلد فبيكون
+
+157
+00:11:54,560 --> 00:12:00,280
+الجلد باهت و خشن و جاف كمان فقد او دفشانس ل
+
+158
+00:12:00,280 --> 00:12:04,970
+vitamin A بيعمل night blindnessاللي هو العشاء
+
+159
+00:12:04,970 --> 00:12:10,310
+الليلي ان الرؤية بالليل بتكون خافتة طب نيجي
+
+160
+00:12:10,310 --> 00:12:13,270
+للفيتامين التاني من الفات سوليو بالفيتامين اللي هو
+
+161
+00:12:13,270 --> 00:12:18,670
+فيتامين دهفيتامين دال بيصنع في الجسم body can make
+
+162
+00:12:18,670 --> 00:12:23,070
+فيتامين دال من أشعة الشمس بياخد من أشعة الشمس و
+
+163
+00:12:23,070 --> 00:12:26,570
+بيقوم فيتامين دال و كمان حكينا قبل هيك أنه
+
+164
+00:12:26,570 --> 00:12:31,670
+الكلسترول يعتبر الوحدة البنائية لتكوين فيتامين دال
+
+165
+00:12:31,670 --> 00:12:35,690
+إذا ففيتامين دال بياخدوا من أشعة الشمس و من
+
+166
+00:12:35,690 --> 00:12:40,570
+الكلسترول بتم تصنيع في الكيليا و الكبد in liver
+
+167
+00:12:40,570 --> 00:12:45,410
+and kidneyلو صار عندى اى مرض فى ال liver و ال
+
+168
+00:12:45,410 --> 00:12:49,150
+kidney هاد هيأثر على ال activation على تكوين
+
+169
+00:12:49,150 --> 00:12:53,610
+فيتامين ده لذن اذا صار اى مرض فى الكلية و الكبد
+
+170
+00:12:53,610 --> 00:12:59,470
+هيأثر على إنتاج فيتامين ده source of vitamin ده
+
+171
+00:12:59,470 --> 00:13:03,570
+لون الليمون موجود في vitamin ده لون موجود؟ موجود
+
+172
+00:13:03,570 --> 00:13:07,310
+فى ال fortified food فى الملك فى ال margarine فى
+
+173
+00:13:07,310 --> 00:13:10,750
+ال beef فى ال eggفي ال sun، من أشعة الشمس، في
+
+174
+00:13:10,750 --> 00:13:15,210
+الحليب، في ال margarine، في ال بيف البقرية، موجود
+
+175
+00:13:15,210 --> 00:13:19,810
+في أماكن، في أطعمة متعددة، مش مطلوب مننا نحفظ هذه
+
+176
+00:13:19,810 --> 00:13:26,630
+الأطعمة هذه صورة بتوضح كيف يتم إنتاج فيتامين دال،
+
+177
+00:13:26,630 --> 00:13:30,310
+مش مطلوبة مننا برضه، بس مطلوب مننا أن نعرف أن
+
+178
+00:13:30,310 --> 00:13:34,210
+فيتامين دال بيصنع في الجسم من أشعة الشمس و من
+
+179
+00:13:34,210 --> 00:13:36,950
+الكوليسترول في ال liver و ال kidney
+
+180
+00:13:39,720 --> 00:13:44,840
+vitamin dal function إيش وظائف vitamin dal؟ أول
+
+181
+00:13:44,840 --> 00:13:49,220
+وظيفة part of the bone making maintenance team هو
+
+182
+00:13:49,220 --> 00:13:54,300
+أحد عضاء فريق المحافظة على العضم، إله دور مهم في
+
+183
+00:13:54,300 --> 00:14:00,010
+المحافظة على العظامكيف بيحافظ على العضام؟ maintain
+
+184
+00:14:00,010 --> 00:14:03,330
+blood concentration of calcium and phosphorus
+
+185
+00:14:03,330 --> 00:14:07,890
+بيحافظ على مستوى مناسب من الكالسيوم و الفوسفوراس
+
+186
+00:14:07,890 --> 00:14:14,490
+في الدم و
+
+187
+00:14:14,490 --> 00:14:18,130
+الكالسيوم عضو مهم لبناء العضم و الفوسفوراس
+
+188
+00:14:18,510 --> 00:14:23,250
+mineralization of bone بيعمل على تمادن العظام
+
+189
+00:14:23,250 --> 00:14:28,210
+تمادن العظيم إيش يعني تمادن العظيم تمادن العظيم
+
+190
+00:14:28,210 --> 00:14:31,430
+raise blood calcium and phosphorus by increasing
+
+191
+00:14:31,430 --> 00:14:35,990
+absorption from gas digestive tract أنه بيرفع
+
+192
+00:14:35,990 --> 00:14:40,430
+مستوى ال calcium و ال phosphorus بزيادة انتصاصه عن
+
+193
+00:14:40,430 --> 00:14:46,310
+طريق انه يزيد انتصاصهإذا تمعدن العظم أني أزود كمية
+
+194
+00:14:46,310 --> 00:14:49,750
+الكالسيوم الفوسفوراس في العظام اللي بتجوه العظم
+
+195
+00:14:49,750 --> 00:14:54,070
+كيف بدي أرفع هذه؟ بعمل على زيادة امتصاص الكالسيوم
+
+196
+00:14:54,070 --> 00:14:58,130
+الفوسفوراس من الأمعاء للدم ان امتص الكالسيوم
+
+197
+00:14:58,130 --> 00:15:02,130
+الفوسفوراس من الأمعاء للدم Deposition of calcium
+
+198
+00:15:02,130 --> 00:15:05,850
+and phosphorus into bone ويسحب الكالسيوم
+
+199
+00:15:05,850 --> 00:15:10,710
+الفوسفوراس ويعملهم Deposition تراكم في العظم إنهم
+
+200
+00:15:10,710 --> 00:15:16,440
+ييجوا على العظمStimulating retention by kidney شو
+
+201
+00:15:16,440 --> 00:15:19,800
+يعني stimulating retention؟ ال retention أني أسحب
+
+202
+00:15:19,800 --> 00:15:23,900
+الكالسيوم و الفوسفرس من الكيلية للدم بدل ما يتم
+
+203
+00:15:23,900 --> 00:15:29,400
+إخراجه مع البال لكي الكيلية ال kidney توصلها فضلات
+
+204
+00:15:29,400 --> 00:15:32,570
+و بقايا الجسم اللي بتعملها يخرج مع ال urineجابل ما
+
+205
+00:15:32,570 --> 00:15:37,010
+تعمل إخراج هب يعمل فيتامين دال على ال retention
+
+206
+00:15:37,010 --> 00:15:41,370
+أنه يسحب الكالسيوم و الفوسفورس من الكيليا إلى الدم
+
+207
+00:15:41,370 --> 00:15:47,060
+يرجع تاني بدل ما يتم إخراجه مع ال bonedeficiency
+
+208
+00:15:47,060 --> 00:15:51,500
+لو قال فيتامين دال أهم الأسباب الأمراض اللى هتسير
+
+209
+00:15:51,500 --> 00:15:56,340
+أنه هيأثر على العظم هو بيصير infection بيصير عندى
+
+210
+00:15:56,340 --> 00:16:01,480
+عدوى و أهم إشي أنه بيصير عندى ostomalgia أو ركيزيا
+
+211
+00:16:01,480 --> 00:16:04,960
+إيش هي ال ostomalgia و الركيزيا؟ هذا الكساح
+
+212
+00:16:04,960 --> 00:16:09,480
+الركيزيا هو الكساح عند ال child و ال infant عند
+
+213
+00:16:09,480 --> 00:16:15,190
+الصغار السان ركيزيايطلق على الكساح عند صغار السن
+
+214
+00:16:15,190 --> 00:16:20,210
+استريو ماليزيا هو الكساح عند كبار السن عند ال
+
+215
+00:16:20,210 --> 00:16:26,550
+adult بيكون عند ال adult طب نحكي عن فيتمين E تالت
+
+216
+00:16:26,550 --> 00:16:30,850
+واحد من الفئة السليف الفيتامين هو فيتمين E فيتمين
+
+217
+00:16:30,850 --> 00:16:36,630
+E يعتبر antioxidant يعتبر antioxidant ايش يعني
+
+218
+00:16:36,630 --> 00:16:42,970
+antioxidant؟Antioxidant يعني ضد الأكسدة ضد الأكسدة
+
+219
+00:16:42,970 --> 00:16:47,230
+ضد الأكسدة يعني بتتطبط عملية الأكسدة Antioxidant
+
+220
+00:16:47,230 --> 00:16:49,950
+is a molecule that inhibit the oxidation of
+
+221
+00:16:49,950 --> 00:16:53,570
+another molecule بتطبط عملية الأكسدة Defender
+
+222
+00:16:53,570 --> 00:16:57,270
+against free radical كيف هي ال antioxidant بتكون
+
+223
+00:16:57,270 --> 00:17:01,950
+ضرورية للجسم؟انها بتدافع عن الجسم ضد ال free
+
+224
+00:17:01,950 --> 00:17:06,390
+radical ايش هي ال free radical انه لما يدخل فيروس
+
+225
+00:17:06,390 --> 00:17:10,130
+او بكتيريا للجسم لداخل الجسم يدخل كأن غريب زي
+
+226
+00:17:10,130 --> 00:17:14,270
+فيروس او بكتيريا الجسم بيروح بيقاوم هذا الفيروس او
+
+227
+00:17:14,270 --> 00:17:19,100
+بكتيريا كبدي قاومه وبينتج ال free radicalfree
+
+228
+00:17:19,100 --> 00:17:22,820
+radical هي دي عشان يتهاجم الفيروس و البكتيريا لكن
+
+229
+00:17:22,820 --> 00:17:26,860
+عيوبها انها بتهاجم كمان خلايا الجسم هي هتهاجم
+
+230
+00:17:26,860 --> 00:17:30,900
+الفيروس و البكتيريا و تقضي عليه و تهاجم كمان خلايا
+
+231
+00:17:30,900 --> 00:17:36,520
+الجسم يعني هتضر خلايا الجسم فإيش بيجي vitamin E
+
+232
+00:17:36,520 --> 00:17:41,140
+بيعمل اختزال لهذه free radical هو ضد ال X ده فإيش
+
+233
+00:17:41,140 --> 00:17:45,960
+هيعمل اختزال بيختزل ال free radical و بيوقف نشاطها
+
+234
+00:17:47,260 --> 00:17:51,680
+فهو Defender against free radical بيدافع عن الجسم
+
+235
+00:17:51,680 --> 00:17:55,520
+من ال free radical عشان ما تأثرش عليه may reduce
+
+236
+00:17:55,520 --> 00:18:00,020
+the risk of heart disease بقلل خطر الإصابة بأمراض
+
+237
+00:18:00,020 --> 00:18:04,540
+القلب widespread in food منتشر بشكل واسع في أطعمة
+
+238
+00:18:04,540 --> 00:18:09,760
+كثيرةeasily destroyed by heat processing الحرارة
+
+239
+00:18:09,760 --> 00:18:14,920
+بتأثر عليه vitamin E الحرارة بتأثر عليه وحساس
+
+240
+00:18:14,920 --> 00:18:20,120
+للحرارة بنطلق عليه heat sensitive ال deficiency ال
+
+241
+00:18:20,120 --> 00:18:24,000
+deficiency له برضه rare نادرة ما يحدث deficiency
+
+242
+00:18:24,000 --> 00:18:29,040
+لأن هو عبارة عن fat soluble vitamins بتم تخزين ال
+
+243
+00:18:29,040 --> 00:18:33,160
+excess منه بتم تخزينه في الجسم فإن يفقده أو أنه
+
+244
+00:18:33,160 --> 00:18:38,650
+يتم نقص فيه هذانادرا إيه لو صار deficientiation
+
+245
+00:18:38,650 --> 00:18:42,170
+مرض ممكن يصير بيصير عندي erythrocyte hemolysis
+
+246
+00:18:42,170 --> 00:18:47,670
+erythrocyte hemolysis هي تكسير لخلايا الدم الحمراء
+
+247
+00:18:47,670 --> 00:18:56,550
+تعني تكسير لخلايا الدم الحمراء vitamin K vitamin K
+
+248
+00:18:56,550 --> 00:19:00,250
+برضه fat-soluble vitamin aid in blood declutting
+
+249
+00:19:00,250 --> 00:19:04,750
+and bone mineralization بيلعب دور في تجلوت الدم
+
+250
+00:19:05,020 --> 00:19:11,380
+وتمادن العظم دور vitamin K بيلعب دور في تجلط الدم
+
+251
+00:19:11,380 --> 00:19:16,040
+وتمادن العظم deficiency causes hemorrhagic disease
+
+252
+00:19:16,040 --> 00:19:22,480
+إذا صار فيه نقص هيصير أمراض نزيفية أمراض نزف ليش
+
+253
+00:19:22,480 --> 00:19:25,680
+لأن دوره أنه يلعب دور في تجلط الدم وتخسر الدم إذا
+
+254
+00:19:25,680 --> 00:19:30,190
+نقص بديصير أمراض نزيف مش تجلط للدم فيه نزيفsource
+
+255
+00:19:30,190 --> 00:19:34,610
+من وين بنحصل على vitamin K هو vitamin بتم تصنيعه
+
+256
+00:19:34,610 --> 00:19:37,450
+من البكتيريا الموجودة في اللمعاء من ال
+
+257
+00:19:37,450 --> 00:19:41,990
+gastrointestinal tract و بتم امتصاصه وتخزينه في ال
+
+258
+00:19:41,990 --> 00:19:47,050
+liver إذا مصدره من بكتيريا موجودة في اللمعاء و بتم
+
+259
+00:19:47,050 --> 00:19:52,270
+امتصاصه وتخزينه في ال liver بيستخدم لتصنيع عوامل
+
+260
+00:19:52,270 --> 00:19:57,630
+التجلط ال clothing factorهك بنقول خلصنا الفات
+
+261
+00:19:57,630 --> 00:20:01,130
+سوليوبل فيتامين و حكينا عن كل واحد منهم على السريع
+
+262
+00:20:01,130 --> 00:20:03,810
+اللي هتنيجي على القسم التاني اللي هم ال water
+
+263
+00:20:03,810 --> 00:20:08,170
+soluble vitamin ال water soluble vitamin هم
+
+264
+00:20:08,170 --> 00:20:13,590
+vitamin B و C بس B بيحكوا عنها B complex لأنهم
+
+265
+00:20:13,590 --> 00:20:19,010
+عبارة عن 8 أنواع من B بس بنطلق عليهم باسم B كلهم B
+
+266
+00:20:19,010 --> 00:20:23,630
+لكن هم B complex عددهم 8 مع vitamin C عن ال water
+
+267
+00:20:23,630 --> 00:20:29,450
+soluble vitamin بيطلعوا 9خصائص الـ water soluble
+
+268
+00:20:29,450 --> 00:20:33,890
+vitamins اللي حكيناها في الجدول إنها لا تلعب دور
+
+269
+00:20:33,890 --> 00:20:38,870
+في إنتاج الطاقة إنه هي non energy producing لا
+
+270
+00:20:38,870 --> 00:20:45,010
+تنتج الطاقة ولكن تساهم في إنتاج الطاقةهي بتساعد كـ
+
+271
+00:20:45,010 --> 00:20:49,010
+Co-factor بتساعد الإنزيم على إنتاج الطاقة
+
+272
+00:20:49,010 --> 00:20:52,630
+بيكومplex فيتمين are often associated with giving
+
+273
+00:20:52,630 --> 00:20:57,770
+a person more energy بتساهم في إنتاج الطاقة فبتزود
+
+274
+00:20:57,770 --> 00:21:01,030
+طاقة this is due to the fact this vitamin each
+
+275
+00:21:01,030 --> 00:21:04,370
+play different role in energy metabolism أنها
+
+276
+00:21:04,370 --> 00:21:08,570
+بتلعب كيف بتساهم بإعطاء الشخص الطاقة ليس عن طريق
+
+277
+00:21:08,570 --> 00:21:13,840
+إنتاج الطاقة وإنهي بتنتجش الطاقة من نفسها، هي
+
+278
+00:21:13,840 --> 00:21:17,920
+نفسها تزوّد الجسم بالطاقة، لأ وإنما إنها تساهم كـ
+
+279
+00:21:17,920 --> 00:21:24,640
+Co-factor كمساعد لإنتاج الطاقةبالرغم من أن هذه
+
+280
+00:21:24,640 --> 00:21:31,460
+الفيطامينات مميزة بالماء، لا تتم تخزينها في الجسم
+
+281
+00:21:31,460 --> 00:21:35,740
+حكيينة، بتم إخراج الزائد منها عبر الكدن مع
+
+282
+00:21:35,740 --> 00:21:39,440
+اليورين، تتدخل في الماء، وتتدخل في الماء، وتتم
+
+283
+00:21:39,440 --> 00:21:42,840
+إكسكريتها من الجسم من خلال اليورين، من خلال
+
+284
+00:21:42,840 --> 00:21:46,600
+اليورين، لذلك، من المهم أن تتناول الطعام مليء في
+
+285
+00:21:46,600 --> 00:21:51,280
+هذه الفيطامينات كل يوم، لكي توفر الجسم ما يحتاجه
+
+286
+00:21:51,760 --> 00:21:55,880
+إذا ان هي حاجة ان انا ولا تخزن في الجسم بدوف المي
+
+287
+00:21:55,880 --> 00:21:59,580
+يتم إخراجها مع ال urine لازم أتناولها كل فترة من
+
+288
+00:21:59,580 --> 00:22:06,260
+يوم لتلت أيام عشان أقضي حاجة الجسممن الأمثلة على
+
+289
+00:22:06,260 --> 00:22:09,100
+ال water-soluble vitamin في عندنا ال بيكمبليكس
+
+290
+00:22:09,100 --> 00:22:15,340
+فيتامين ال بيكمبليكس يعتبر ك coenzyme يعني بيحفظ
+
+291
+00:22:15,340 --> 00:22:20,440
+ال انزيم found in the same food ال بيكمبليكس
+
+292
+00:22:20,440 --> 00:22:25,410
+فيتامين هم كمبليكس عبارة عن تمانيةكلهم موجودين في
+
+293
+00:22:25,410 --> 00:22:29,490
+نفس الطعام يعني لو قلتلك واحدة نوع مثلا في ال B
+
+294
+00:22:29,490 --> 00:22:32,990
+-complex الثيامين إذا الثيامين موجود في الجزر يبدأ
+
+295
+00:22:32,990 --> 00:22:36,710
+كل ال B-complex التمانية كلهم موجودين في الجزر فال
+
+296
+00:22:36,710 --> 00:22:40,970
+single deficiency rare أني أفقد واحد منهم هذا إشي
+
+297
+00:22:40,970 --> 00:22:46,580
+نادر Act together in metabolism بتلعب دورفي الـ
+
+298
+00:22:46,580 --> 00:22:50,920
+Metabolism في إنتاج الطاقة Metabolic pathway used
+
+299
+00:22:50,920 --> 00:22:54,960
+by protein, carbohydrate and fat حكينا قبل هيك أن
+
+300
+00:22:54,960 --> 00:22:58,180
+مصدر الطاقة الـ carbohydrate ومخزن الطاقة هي
+
+301
+00:22:58,180 --> 00:23:01,980
+الدهون وطاقة الخلايا للبناء هي البروتين يعني هدول
+
+302
+00:23:01,980 --> 00:23:07,550
+مسئولين عن إنتاج الطاقة للجسم الـ Vitaminبتساهم في
+
+303
+00:23:07,550 --> 00:23:10,030
+إنتاج الطاقة، بتساعد هدول الـ carbohydrate
+
+304
+00:23:10,030 --> 00:23:13,730
+والبروتين والفت على إنهم يعطوا الطاقة للجسم، لكن
+
+305
+00:23:13,730 --> 00:23:19,110
+هي بنفسها لا تنتج الطاقة مين هما الـ B-complex
+
+306
+00:23:19,110 --> 00:23:22,830
+vitamin؟ فيها الناس صيامين، اللي هو B1, riboflavin
+
+307
+00:23:22,830 --> 00:23:28,830
+B2, niacin B3, pantothenic acid, biotin, pyridoxin
+
+308
+00:23:28,830 --> 00:23:33,570
+B6 و الـ folate و vitamin B12، هدولة كلهم vitamin
+
+309
+00:23:33,570 --> 00:23:38,380
+Bفهم أكتر من نوع أطلقنا عليهم B Complex Vitamin
+
+310
+00:23:38,380 --> 00:23:45,280
+وكلهم موجودين في نفس نوع الطعام إيش وظائف ال B
+
+311
+00:23:45,280 --> 00:23:49,720
+Complex؟ أول وظيفة هي Energy Metabolism حكينا أنها
+
+312
+00:23:49,720 --> 00:23:54,670
+بتساهم في إنتاج الطاقةو هنجسم اللي هيهم مين منهم
+
+313
+00:23:54,670 --> 00:23:57,830
+اللي بتساهم في إنتاج الطاقة و هذا مش مطلوب منها،
+
+314
+00:23:57,830 --> 00:24:02,110
+مطلوب منها نعرف بس الوظائف كعنوين ال بيك كومبليكس
+
+315
+00:24:02,110 --> 00:24:05,490
+أول وظيفة إلها energy metabolism إنتاج الطاقة
+
+316
+00:24:05,490 --> 00:24:09,910
+تساهم في إنتاج الطاقة red blood cell sensitives
+
+317
+00:24:09,910 --> 00:24:13,970
+إنه بتساهم في إنتاج خلايا الدم الحمراء كوريات الدم
+
+318
+00:24:13,970 --> 00:24:18,960
+الحمراءHomocysteine Metabolism اللي هو عملية
+
+319
+00:24:18,960 --> 00:24:23,100
+Metabolism لحاجة نسميها الـ Homocysteine
+
+320
+00:24:26,000 --> 00:24:31,860
+vitamin C اسمه الكيميائي بنطلق عليه اسم ascorbic
+
+321
+00:24:31,860 --> 00:24:36,820
+acid ascorbic acid او vitamin C senses by most
+
+322
+00:24:36,820 --> 00:24:42,060
+animal بتتم تصنيعه من معظم الحيوانات لكن الإنسان
+
+323
+00:24:42,060 --> 00:24:47,000
+لأ كل الحيوانات بتصنعه ما عدا الإنسان الإنسان غير
+
+324
+00:24:47,000 --> 00:24:51,460
+قادر على تصنيع vitamin Cdecrease absorption with
+
+325
+00:24:51,460 --> 00:24:56,220
+high intake اكيد هيقلل الامتصاص اذا انا عند زيادة
+
+326
+00:24:56,220 --> 00:24:59,900
+التناولة اذا الجسم حاسس ان هو موجود بكميات كافية
+
+327
+00:24:59,900 --> 00:25:04,620
+هيقلل الامتصاص ال excess منه الكميات الزائدة هيصير
+
+328
+00:25:04,620 --> 00:25:10,660
+execution عملية اخراج لها food source of vitamin C
+
+329
+00:25:10,660 --> 00:25:16,890
+وين موجود؟ موجود في الcitrus .. citrus fruit اللي
+
+330
+00:25:16,890 --> 00:25:20,890
+هي الفواكه الحمضية موجود في ال .. في ال potato، في
+
+331
+00:25:20,890 --> 00:25:24,230
+ال green pepper، في الفلفل الأخضر، في الزهرة، في
+
+332
+00:25:24,230 --> 00:25:27,470
+ال broccoli، في ال strawberry، فالورة، في ال
+
+333
+00:25:27,470 --> 00:25:31,350
+romaine lettuce، الخس، في ال spinach، السبانخ، مش
+
+334
+00:25:31,350 --> 00:25:36,830
+مطلوب إننا نحفظ اللي أطعمهاfunction of vitamin C،
+
+335
+00:25:36,830 --> 00:25:41,650
+إيش وظائف vitamin C؟ vitamin C يعتبر ضي vitamin E،
+
+336
+00:25:41,650 --> 00:25:46,950
+إن هو reducing agent، يعني antioxidant، vitamin C
+
+337
+00:25:46,950 --> 00:25:53,350
+و E يعتبروا antioxidant reducing agentأيش وظيفته؟
+
+338
+00:25:53,350 --> 00:25:58,170
+بيحفظ ال iron absorption enhanced يحفظ انتصاص
+
+339
+00:25:58,170 --> 00:26:03,550
+الحديد، يحفظ انتصاص الحديد عشان هيك مثلا لو واحد
+
+340
+00:26:03,550 --> 00:26:07,590
+عنده نقص في الحديد، بيعطوه حبوب حديد، بيحكولي يفضل
+
+341
+00:26:07,590 --> 00:26:11,850
+لو تتناول الحبوب الحديد مع عصير برتقال عشان عصير
+
+342
+00:26:11,850 --> 00:26:16,130
+البرتقال يحتوي على vitamin C و vitamin C بيحفظ
+
+343
+00:26:16,130 --> 00:26:21,280
+الانتصاصSynthesis of collagen كمان هو مسئول عن
+
+344
+00:26:21,280 --> 00:26:25,060
+تصنيع الكولاجين اللى موجود فى الجلد Immune
+
+345
+00:26:25,060 --> 00:26:29,940
+function اللى وظائف منعية ايش وظيفته المنعية بيحكى
+
+346
+00:26:29,940 --> 00:26:33,160
+اللى doesn't prevent common cold or flu but may
+
+347
+00:26:33,160 --> 00:26:37,420
+reduce duration of symptom day by day ان هو راجيت
+
+348
+00:26:37,420 --> 00:26:40,700
+لما الواحد يصير معاه influenza بيحكوله اشرب عصير
+
+349
+00:26:40,700 --> 00:26:46,520
+برتقال او اتناول البرتقال هذا بيجلل اللى هو الفترة
+
+350
+00:26:47,240 --> 00:26:50,940
+Vitamin C الموجود في عصير البرتقال بيجلل فترة
+
+351
+00:26:50,940 --> 00:26:55,760
+أعراض المرض ولا يمنع ال influenza وعراض ال burn
+
+352
+00:26:55,760 --> 00:27:01,280
+وإنما يقلل فترة هذه الأعراضإذا لا يقضي على الفيروس
+
+353
+00:27:01,280 --> 00:27:05,420
+وإنما يقلل الفترة كمان و من وضع فيه wound healing
+
+354
+00:27:05,420 --> 00:27:09,280
+اللي هو التقام الجروح يساعد في التقام الجروح
+
+355
+00:27:09,280 --> 00:27:12,840
+easily lost through cooking برضه هو عبارة عن heat
+
+356
+00:27:12,840 --> 00:27:17,880
+sensitive حساس للحرارة فبيفقد مع درجات الحرارة
+
+357
+00:27:17,880 --> 00:27:22,500
+العالية لإن لو عصير البرتقال سخنتي خلاص vitamin C
+
+358
+00:27:22,500 --> 00:27:26,400
+بيتكسر بيروح مش هيدل موجود sensitive to heat
+
+359
+00:27:28,720 --> 00:27:33,140
+vitamin C deficiency إذا صار عندي فقد أو نقص فيه
+
+360
+00:27:33,140 --> 00:27:38,620
+vitamin C أشهر مرض بينتج عن نقص vitamin C هو مرض
+
+361
+00:27:38,620 --> 00:27:42,860
+الـscurvy الاسقربوت مرض scurvy disease اللي هو
+
+362
+00:27:42,860 --> 00:27:47,620
+الاسقربوت disease characterized by weakness, small
+
+363
+00:27:47,620 --> 00:27:52,960
+hemorrhage throughout the body that cause gum and
+
+364
+00:27:52,960 --> 00:27:58,030
+skin to bleedهو مرض بيكون عبارة عن خصائصه أنه
+
+365
+00:27:58,030 --> 00:28:03,690
+بيكون ضعف عام في الجسم، بيكون فيه نزيف في مسامات
+
+366
+00:28:03,690 --> 00:28:08,390
+الجلد، مسامات الجلد بتفرز دم، ممكن نزيف في اللثة و
+
+367
+00:28:08,390 --> 00:28:14,580
+سقوط الأسنانفي عندنا صورة تحت مثلا Sclerotic
+
+368
+00:28:14,580 --> 00:28:18,580
+Rosary، هاي من المرض أنه بيكون عندنا بروز للقفص
+
+369
+00:28:18,580 --> 00:28:22,840
+الصدري، القفص الصدري بارز للأمام، في عندنا بيكون
+
+370
+00:28:22,840 --> 00:28:28,460
+نزيف في اللثة، تساقط للأسنان، في عندنا Volicular
+
+371
+00:28:28,460 --> 00:28:32,580
+Hemorrhage، اللي هو نزيف في مسامات الجلب، تحت
+
+372
+00:28:32,580 --> 00:28:38,820
+الجلب بيلاقي بُقع زي نزيفVitamin C excess لو كان
+
+373
+00:28:38,820 --> 00:28:43,940
+في عندنا كميات زائدة من vitamin C اللي هو زيادة في
+
+374
+00:28:43,940 --> 00:28:47,940
+حاجة الجسم وهي زيادة أكيد rare لأن هو water
+
+375
+00:28:47,940 --> 00:28:51,740
+soluble vitamin بيصيرله excretion بيصيرله إخراج بس
+
+376
+00:28:51,740 --> 00:28:56,920
+إذا كان بكميات زائدة بسبب خلال ماء بيصير عندي مرض
+
+377
+00:28:56,920 --> 00:29:02,710
+hemochromatosisHemochromatosis معناها زيادة نسبة
+
+378
+00:29:02,710 --> 00:29:07,150
+الحديد في الجسم زيادة تخزين الحديد Iron overload
+
+379
+00:29:07,150 --> 00:29:12,250
+لأنه حكينا أن Vitamin C بيحفظ امتصاص الحديد فإذا
+
+380
+00:29:12,250 --> 00:29:16,710
+زاد Vitamin C هيمتص كمية من الحديد الزيادة هيزود
+
+381
+00:29:16,710 --> 00:29:20,910
+كمية امتصاص الحديد وسبب مرض اسمه Hemochromatosis
+
+382
+00:29:20,910 --> 00:29:27,600
+اللي هو Iron overload زيادة الحديدأجزالات كيدنستون
+
+383
+00:29:27,600 --> 00:29:32,140
+اللي هي حصاوي في الكيلة من نوع أجزالات أجزالات
+
+384
+00:29:32,140 --> 00:29:35,900
+كيدنستون اللي هي حصاوي من نوع أجزالات ايه ال tooth
+
+385
+00:29:35,900 --> 00:29:40,720
+enamel؟ اللي هو تآكل للأسنان، بيصير عندي تآكل
+
+386
+00:29:40,720 --> 00:29:42,080
+للأسنان
+
diff --git a/PL9fwy3NUQKwZ1Q7zOg1ObgufPMjRI3WY1/h8ODbKj_UcE.srt b/PL9fwy3NUQKwZ1Q7zOg1ObgufPMjRI3WY1/h8ODbKj_UcE.srt
new file mode 100644
index 0000000000000000000000000000000000000000..6d0c21c7b79a044a29e5680078b89ba8fa390d14
--- /dev/null
+++ b/PL9fwy3NUQKwZ1Q7zOg1ObgufPMjRI3WY1/h8ODbKj_UcE.srt
@@ -0,0 +1,1351 @@
+1
+00:00:00,000 --> 00:00:04,700
+نحكي في آخر معمل عن اللي هو عبارة عن معمل energy
+
+2
+00:00:04,700 --> 00:00:09,080
+metabolism in yeast تفاعلات إنتاج الطاقة في
+
+3
+00:00:09,080 --> 00:00:12,900
+الكائنات الحية والكائن الحي اللي هنحكي عنه اليوم
+
+4
+00:00:12,900 --> 00:00:18,510
+هو الخميرة. أولا نحكي عن الـ Cellular Respiration
+
+5
+00:00:18,510 --> 00:00:22,690
+اللي هو التنفس الخلوي. إيش هو التنفس الخلوي؟ هو a set
+
+6
+00:00:22,690 --> 00:00:26,170
+of metabolic reactions and processes that take
+
+7
+00:00:26,170 --> 00:00:29,330
+place in the cells of organisms to convert
+
+8
+00:00:29,330 --> 00:00:34,150
+biochemical energy from nutrients into ATP. مجموعة
+
+9
+00:00:34,150 --> 00:00:38,550
+من التفاعلات الحيوية في داخل خلايا الكائنات الحية
+
+10
+00:00:38,550 --> 00:00:45,280
+للحصول على الطاقة أو إنتاج الطاقة على شكل ATP من
+
+11
+00:00:45,280 --> 00:00:49,960
+الطعام اللي بنتناوله. إذا هي تفاعلات حيوية في داخل
+
+12
+00:00:49,960 --> 00:00:54,980
+خلايا الكائنات الحية لإنتاج الطاقة على شكل ATP من
+
+13
+00:00:54,980 --> 00:01:00,400
+الطعام اللي بنتناوله. تفاعلات إنتاج الطاقة تعتبر
+
+14
+00:01:00,400 --> 00:01:05,980
+catabolic reactions، تفاعلات هدم وليس تفاعلات بناء، 
+
+15
+00:01:05,980 --> 00:01:09,180
+break large molecules into smaller ones releasing
+
+16
+00:01:09,180 --> 00:01:14,180
+energy. عملية تكسير مركب كبير إلى صغير بينتج
+
+17
+00:01:14,180 --> 00:01:19,120
+عنها طاقة. نحكي عن الكائن الحي اللي بندرسه اليوم
+
+18
+00:01:19,120 --> 00:01:21,880
+اللي هو الـ yeast. اسمها العلمي Saccharomyces
+
+19
+00:01:21,880 --> 00:01:26,420
+cerevisiae. الخميرة هي living organisms that require a
+
+20
+00:01:26,420 --> 00:01:29,660
+warm, moist environment and a food source to grow
+
+21
+00:01:29,660 --> 00:01:34,100
+and thrive. هي الكائن الحي بيحتاج إلى بيئة دافئة و
+
+22
+00:01:34,100 --> 00:01:41,100
+رطبة، ويحتاج إلى مصدر طعام لنمو والمعيشة عشان
+
+23
+00:01:41,100 --> 00:01:46,140
+تنمو وتعيش. بدها بيئة رطبة تكون ودافئة وبدها
+
+24
+00:01:46,140 --> 00:01:52,060
+مصدر غذاء. هي unicellular أحدية الخلية/single cell
+
+25
+00:01:52,060 --> 00:01:56,920
+facultative anaerobic fungi. هي خلايا بتعيش في وجود
+
+26
+00:01:56,920 --> 00:02:00,860
+هي كائنات حية تعيش في وجود الـ oxygen أو عدم وجود
+
+27
+00:02:00,860 --> 00:02:04,920
+الـ oxygen. هي اختيارية تعيش في وجود الـ oxygen أو
+
+28
+00:02:04,920 --> 00:02:09,880
+عدم وجود الـ oxygen. تحت الـ microscope كيف شكلها
+
+29
+00:02:09,880 --> 00:02:14,380
+بيكون، بيكون شكلها oval بيدوي وإذا كانت بتنقسم
+
+30
+00:02:14,380 --> 00:02:21,150
+بتكون مطلعة برعم. بضم cellular respiration بيحكي
+
+31
+00:02:21,150 --> 00:02:24,450
+لأنه في هذا المعمل we will be using yeast to measure
+
+32
+00:02:24,450 --> 00:02:27,770
+the rate of cellular respiration. لنستخدم الخميرة
+
+33
+00:02:27,770 --> 00:02:32,050
+عشان نقيس معدل التنفس الخلوي عندها. كده إيش معدل
+
+34
+00:02:32,050 --> 00:02:37,010
+التنفس الخلوي عند الخميرة؟ الخميرة هي single cell هي
+
+35
+00:02:37,010 --> 00:02:41,590
+حكينا أنها أحدية الخلية. eukaryotic organism حقيقية
+
+36
+00:02:41,590 --> 00:02:46,410
+النواة. أنها تستهلك الكربوهيدرات/السكريات عشان تنتج الـ
+
+37
+00:02:46,410 --> 00:02:50,810
+ATP. نفس الطريقة زيها زي النبات والحيوان.
+
+38
+00:03:00,090 --> 00:03:04,710
+الخميرة بتحول السكر لمياه و Carbon Dioxide عشان
+
+39
+00:03:04,710 --> 00:03:09,730
+تحصل على الطاقة. إذا السكر بيدخل وبينتج عنه by
+
+40
+00:03:09,730 --> 00:03:12,610
+product من الـ cellular respiration بينتج عنه ماء
+
+41
+00:03:12,610 --> 00:03:19,100
+water (H2O) و CO2 و Carbon Dioxide و ATP طاقة. The
+
+42
+00:03:19,100 --> 00:03:21,760
+greater the carbon dioxide production, the greater
+
+43
+00:03:21,760 --> 00:03:25,460
+the rate of cellular respiration. كل ما كانت كمية
+
+44
+00:03:25,460 --> 00:03:30,260
+الـ CO2 الناتجة أكبر، كمية أكبر، إذا المعدل التنفس 
+
+45
+00:03:30,260 --> 00:03:35,450
+الخلوي اللي صار عندي أكبر. إيش مراحل الـ cellular
+
+46
+00:03:35,450 --> 00:03:39,590
+respiration? stages of cellular respiration  أنت ليها
+
+47
+00:03:39,590 --> 00:03:43,750
+مراحل. أول مرحلة الـ glycolysis اللي بتحدث في الـ
+
+48
+00:03:43,750 --> 00:03:48,050
+cytosol. أول مرحلة glycolysis في الـ cytosol. ثاني
+
+49
+00:03:48,050 --> 00:03:51,430
+مرحلة الـ Krebs Cycle اللي بتحدث في الـ mitochondrial
+
+50
+00:03:51,430 --> 00:03:56,390
+matrix. تالت مرحلة electron transport chain بتحدث في
+
+51
+00:03:56,390 --> 00:04:01,190
+inner membrane of mitochondria. لذا الـ glycolysis
+
+52
+00:04:01,190 --> 00:04:06,070
+في الـ cytosol. Krebs Cycle الـ mitochondrial matrix
+
+53
+00:04:06,070 --> 00:04:11,030
+التجويف الداخلي للميتوكوندريا. electron transport
+
+54
+00:04:11,030 --> 00:04:17,420
+chain بتحدث في الـ membrane الداخلي للميتوكوندريا. أول
+
+55
+00:04:17,420 --> 00:04:21,100
+مرحلة الـ glycolysis هي عملية بتحدث في الـ cytosol
+
+56
+00:04:21,100 --> 00:04:24,960
+زي ما حكينا بتحدث في وجود أو عدم وجود الـ oxygen
+
+57
+00:04:24,960 --> 00:04:28,080
+with or without the presence of oxygen. يعني إذا
+
+58
+00:04:28,080 --> 00:04:32,460
+الجسم محتاج لطاقة، إذا في oxygen أو فش oxygen. أول
+
+59
+00:04:32,460 --> 00:04:35,980
+مرحلة لازم يبدأها هي الـ glycolysis. إيش هذه
+
+60
+00:04:35,980 --> 00:04:40,140
+العملية؟ يتم تحويل one molecule of glucose into two
+
+61
+00:04:40,140 --> 00:04:45,840
+molecules of pyruvate. إنه يكسر سكر الجلوكوز وينتج
+
+62
+00:04:45,840 --> 00:04:50,280
+عنه اثنين بيروفات. هذه العملية بينتج عنها two
+
+63
+00:04:50,280 --> 00:04:55,860
+molecules of ATP. بينتج عنها كم محصلة؟ اثنين ATP. إذا
+
+64
+00:04:55,860 --> 00:04:59,560
+عملية الـGlycolysis تكسير الجلوكوز إلى اثنين
+
+65
+00:04:59,560 --> 00:05:04,640
+بيروفات بينتج عنها، في الحقيقة، بينتج أربعة ATP ولكن
+
+66
+00:05:04,640 --> 00:05:07,980
+هذه العملية بتستهلك منهم اثنين وبيظل عنده اثنين.
+
+67
+00:05:08,560 --> 00:05:12,840
+فهما four molecules of ATP are produced ولكن الـ
+
+68
+00:05:12,840 --> 00:05:17,080
+net number، الـ number of ATP، الجدش العدد هو أربعة لكن
+
+69
+00:05:17,080 --> 00:05:19,980
+الـ net number، الحاصل النهائي اللي طلعنا منها من
+
+70
+00:05:19,980 --> 00:05:21,960
+الطاقة هي اثنين ATP.
+
+71
+00:05:25,180 --> 00:05:28,420
+the fate of pyruvate. هل مصير بعد هذه العملية بعد
+
+72
+00:05:28,420 --> 00:05:31,940
+الخطوة الأولى اللي هي الـ glycolysis اللي حدثت فيه
+
+73
+00:05:31,940 --> 00:05:35,740
+وجود أو عدم وجود الـ oxygen. الـ pyruvate اللي نتج
+
+74
+00:05:35,740 --> 00:05:41,550
+إيش مصيره؟ مصير الـ pyruvate. البيروفيت هذا له مصيرين.
+
+75
+00:05:41,550 --> 00:05:45,890
+إذا في حالة وجود الأكسجين بيروح على جهة. في حالة
+
+76
+00:05:45,890 --> 00:05:49,710
+عدم وجود الأكسجين بيبدأ حاجة ثانية. إذا الأكسجين
+
+77
+00:05:49,710 --> 00:05:54,990
+موجود، هيدخل هذا البيروفيت على الميتوكوندريا يتحول
+
+78
+00:05:54,990 --> 00:05:59,330
+إلى acetyl CoA. الأسيتيل CoA هذه بتدخل على
+
+79
+00:05:59,330 --> 00:06:03,190
+Krebs Cycle. وبعدين خطوة ثالثة اللي هي الـ electron
+
+80
+00:06:03,190 --> 00:06:08,180
+transport chain. أول عملية الجليكوليسية بتحدث في وجود
+
+81
+00:06:08,180 --> 00:06:12,440
+أو عند وجود الأكسجين بيمتج عنها بيروفين، بيروفيتها.
+
+82
+00:06:12,440 --> 00:06:17,680
+إذا في أكسجين هيكمل aerobic respiration، تنفس خلوي
+
+83
+00:06:17,680 --> 00:06:22,640
+هو aerobic respiration اللي هي Krebs Cycle وبعدين
+
+84
+00:06:22,640 --> 00:06:27,040
+electron transport chain. إذا فش أكسجين، هيبدأ عملية
+
+85
+00:06:27,040 --> 00:06:31,340
+اسمها fermentation أو anaerobic respiration نسميها
+
+86
+00:06:31,340 --> 00:06:36,100
+fermentation أو anaerobic respiration. في حالة
+
+87
+00:06:36,100 --> 00:06:39,440
+الحيوان والبلانت والنبات في الـ Anaerobic
+
+88
+00:06:39,440 --> 00:06:43,140
+respiration بينتج عنها lactic acid الناتج النهائي
+
+89
+00:06:43,140 --> 00:06:47,520
+lactic acid. في حالة اليست والبكتيريا الناتج النهائي
+
+90
+00:06:47,520 --> 00:06:51,660
+هو ethanol، هو كحول ethanol. هذا إذا عدم وجود
+
+91
+00:06:51,660 --> 00:06:55,440
+الأكسجين. fermentation في الانمال والبلاند بينتج
+
+92
+00:06:55,440 --> 00:06:58,900
+lactic acid. في اليست والبكتيريا بينتج ethanol أو
+
+93
+00:06:58,900 --> 00:07:04,440
+كحول. فعشان هيك بنفسر أنه ليش لما تبدأ المجهود عند
+
+94
+00:07:04,440 --> 00:07:07,820
+بدء المجهود بتتعب العضلة؟ ليش؟ لأنه ما فيش أكسجين
+
+95
+00:07:07,820 --> 00:07:12,100
+بيوصل للعضلة، فالعضلة هتبدأ لعملية fermentation لأن
+
+96
+00:07:12,100 --> 00:07:15,420
+ما فيش أكسجين. مش هتعمل الـ aerobic respiration
+
+97
+00:07:15,420 --> 00:07:18,340
+هتعمل anaerobic respiration اللي هي fermentation
+
+98
+00:07:18,340 --> 00:07:22,900
+وهي الـ fermentation بينتج عنها lactic acid بيتراكم
+
+99
+00:07:22,900 --> 00:07:27,480
+في العضلات فبتتعب العضلة. هنا مكتوب أن الـ fate of
+
+100
+00:07:27,480 --> 00:07:30,380
+pyruvate. مصير الـ pyruvate بيحكي اللي بيدخل الـ
+
+101
+00:07:30,380 --> 00:07:34,440
+mitochondria بيتحول إلى acetyl CoA، الأسيتيل CoA
+
+102
+00:07:34,440 --> 00:07:38,200
+هو اللي بيبدأ الـ Krebs Cycle. بس المركب اللي
+
+103
+00:07:38,200 --> 00:07:41,820
+بيبدأ الـ Krebs Cycle هو الأسيتيل CoA، هو اللي
+
+104
+00:07:41,820 --> 00:07:45,380
+بيبدأ الـ Krebs Cycle، مش الـ pyruvate. نقطة اثنين وثلاثة بدنا نشوفها.
+
+105
+00:07:45,380 --> 00:07:48,990
+حكي لي وين الـ oxygen is present إذا
+
+106
+00:07:48,990 --> 00:07:52,590
+الـ oxygen موجود هيبدأ aerobic respiration اللي هي
+
+107
+00:07:52,590 --> 00:07:56,110
+Krebs Cycle وبعدين electron transport chain. لو
+
+108
+00:07:56,110 --> 00:07:58,870
+الـ oxygen not present، مش موجود، هيصير عندي
+
+109
+00:07:58,870 --> 00:08:02,450
+fermentation. الـ pyruvate هيبدأ anaerobic
+
+110
+00:08:02,450 --> 00:08:07,760
+respiration. إذا الـ oxygen موجود، الأسيتيل CoA
+
+111
+00:08:07,760 --> 00:08:11,360
+هيحول الـ pyruvate لـ acetyl CoA. acetyl CoA هذا
+
+112
+00:08:11,360 --> 00:08:15,200
+بيبدأ اللي هي الـ citric acid cycle. الـ citric acid
+
+113
+00:08:15,200 --> 00:08:19,620
+cycle هي نفسها الـ Krebs Cycle، اسم ثاني، اللي بتحدث
+
+114
+00:08:19,620 --> 00:08:24,100
+في داخل الـ mitochondrial matrix. بينتج عنها CO2 و
+
+115
+00:08:24,100 --> 00:08:29,340
+NADH. تمام، الطاقة اللي بتنتج عنها على شكل NADH. الـ
+
+116
+00:08:29,340 --> 00:08:33,710
+citric acid cycle هي عبارة عن ثمان خطوات. هي عبارة
+
+117
+00:08:33,710 --> 00:08:38,490
+عن ثمان خطوات بتحتاج إلى إنزيمات و co-enzyme. إذا
+
+118
+00:08:38,490 --> 00:08:41,610
+حولناها لشيء حاجة بيصير عندنا glycolysis على
+
+119
+00:08:41,610 --> 00:08:45,090
+اليسار من البداية. الـ glycolysis بينتج عنها بيروفات
+
+120
+00:08:45,090 --> 00:08:49,110
+بيروفات هذا بيدخل على الميتوكوندريا بتم تحويله إلى
+
+121
+00:08:49,110 --> 00:08:53,270
+acetyl CoA. عملية تحويله إلى acetyl CoA بينتج
+
+122
+00:08:53,270 --> 00:08:59,250
+عنها CO2 و NADH. تمام، هذا الـ acetyl CoA هو اللي
+
+123
+00:08:59,250 --> 00:09:05,870
+هيبدأ بـ Krebs Cycle. ففي عندي اثنين بيروفات لما
+
+124
+00:09:05,870 --> 00:09:10,970
+تحول لـ acetyl CoA نتج عنهم اثنين NADH في Krebs
+
+125
+00:09:10,970 --> 00:09:15,330
+Cycle. الاسم الثاني حكينا citric acid cycle ويلها
+
+126
+00:09:15,330 --> 00:09:21,590
+اسم ثالث TCA (tricarboxylic acid cycle). إذا الـ
+
+127
+00:09:21,590 --> 00:09:25,650
+Krebs Cycle ويلها ثلاث مسميات: Krebs Cycle, citric
+
+128
+00:09:25,650 --> 00:09:29,610
+acid cycle و tricarboxylic acid cycle (TCA).
+
+129
+00:09:30,710 --> 00:09:34,290
+بتحدث في الـ mitochondrial matrix بتحتاج إلى
+
+130
+00:09:34,290 --> 00:09:37,690
+وجود الأكسجين، هذه العملية لا تحدث في عدم وجود
+
+131
+00:09:37,690 --> 00:09:42,030
+الأكسجين، وإنما بتحتاج إلى أكسجين. في كل
+
+132
+00:09:42,030 --> 00:09:49,320
+واحد جلوكوز، بتصير هذه الحلقة مرتين. Krebs Cycle لكل
+
+133
+00:09:49,320 --> 00:09:53,840
+واحد جلوكوز بتصير مرتين. عن كل مرة بينتج one ATP
+
+134
+00:09:53,840 --> 00:09:58,220
+ثلاثة NADH وواحد FADH. كل حلقة كل دورة بينتج
+
+135
+00:09:58,220 --> 00:10:03,200
+one ATP ثلاثة NADH وواحد FADH. طيب، لكل واحد
+
+136
+00:10:03,200 --> 00:10:07,480
+جلوكوز بتصير مرتين يعني كل واحد جلوكوز الناتج
+
+137
+00:10:07,480 --> 00:10:15,000
+هيكون اثنين ATP ستة NADH واثنين FADH. النتيجة هي
+
+138
+00:10:15,000 --> 00:10:20,240
+أنها Krebs Cycle من واحد جليكوز هو اثنين ATP، ستة
+
+139
+00:10:20,240 --> 00:10:27,180
+NADH واثنين FADH. هنا مخطط للعملية ككل. أول شيء
+
+140
+00:10:27,180 --> 00:10:31,280
+أن الجلوكوز، فا الجسم محتاج لطاقة بيبدأ بياخد
+
+141
+00:10:31,280 --> 00:10:34,500
+الجلوكوز هذا وبيعمله glycolysis اللي بتحدث في الـ
+
+142
+00:10:34,500 --> 00:10:38,020
+cytosol خارج الـ mitochondria. بيصير الـ glycolysis
+
+143
+00:10:38,020 --> 00:10:40,960
+بينتج عنه اثنين بيروفات. تحويل الجلوكوز للاثنين
+
+144
+00:10:40,960 --> 00:10:44,620
+بيروفات حكينا إنه يتحول للاثنين بيروفات على اليمين
+
+145
+00:10:44,620 --> 00:10:49,620
+جدش بينتج عندي اثنين ATP كمحصلة من تحويل الجلوكوز
+
+146
+00:10:49,620 --> 00:10:53,040
+إلى اثنين بيروفات. بينتج عندي اثنين ATP واثنين
+
+147
+00:10:53,040 --> 00:10:56,980
+NADH. تمام؟ وجدت الاثنين بيروفات هذا بيدخل
+
+148
+00:10:56,980 --> 00:11:01,380
+الميتوكوندريا اللي بدها يتم تحويله إلى acetyl CoA فتحت
+
+149
+00:11:01,380 --> 00:11:06,980
+البيروفات في عندنا سهمين: سهم CO2 وسهم 2 NADH. هدول
+
+150
+00:11:06,980 --> 00:11:10,700
+من وين أجوا؟ من تحويل البيروفات إلى acetyl CoA
+
+151
+00:11:10,700 --> 00:11:15,440
+فبدنا نكتب تحت البيروفات acetyl CoA. بعدين الـ acetyl
+
+152
+00:11:15,440 --> 00:11:19,280
+CoA بدها تدخل على Krebs Cycle. في الـ Krebs
+
+153
+00:11:19,280 --> 00:11:23,600
+Cycle بتصير عندنا عدة خطوات بينتج عنها أربعة CO2
+
+154
+00:11:23,600 --> 00:11:30,880
+اثنين ATP ستة NADH واثنين FADH2. اللي كانت الـ NADH
+
+155
+00:11:30,880 --> 00:11:34,620
+والـ FADH بيدخلوا للنتجين، اللي كلهم بيدخلوا على
+
+156
+00:11:34,620 --> 00:11:38,360
+electron transport chain على سلسلة نقل الالكترونات
+
+157
+00:11:38,360 --> 00:11:42,520
+اللي بينتج عنها ماء. بتم تحويل الـ NADH والـ FADH
+
+158
+00:11:42,520 --> 00:11:48,220
+H في هذه السلسلة إلى ATP. تمام، اجيت هنا عند جدول
+
+159
+00:11:48,220 --> 00:11:52,160
+ملخص للطاقة. للـ NADH في عملية الـ glycolysis حكينا
+
+160
+00:11:52,160 --> 00:11:56,580
+أنه بينتج عنها اثنين ATP كمحصلة واثنين NADH
+
+161
+00:11:56,580 --> 00:12:00,660
+إذا في الـ glycolysis بينتج عنده اثنين ATP واثنين
+
+162
+00:12:00,660 --> 00:12:04,440
+NADH. بتعرفوا أن الـ NADH إذا بدي أحوله لـ ATP
+
+163
+00:12:04,440 --> 00:12:08,720
+بضربه في ثلاثة. تمام، الـ NADH إذا بدي أحوله لـ ATP
+
+164
+00:12:08,720 --> 00:12:12,360
+بضربه في ثلاثة. لكن هذه الـ NADH اللي نتجت في الـ
+
+165
+00:12:12,360 --> 00:12:16,440
+glycolysis نتجت في الـ cytosol لازم تدخل بالأول على
+
+166
+00:12:16,440 --> 00:12:19,360
+الميتوكوندريا وبعدين على الـ electron transport chain
+
+167
+00:12:19,360 --> 00:12:23,200
+عشان تتحول لـ ATP. هذه الـ NADH كيف بده تدخل لـ
+
+168
+00:12:23,200 --> 00:12:27,400
+الميتوكوندريا؟ في عندها قناتين في ممرين عشان تدخل
+
+169
+00:12:27,400 --> 00:12:32,250
+الميتوكوندريا. في ممر بنسميه malate aspartate shuttle. في ممر
+
+170
+00:12:32,250 --> 00:12:35,590
+اسمه glycerol-phosphate shuttle. إذا إن الـ NADH
+
+171
+00:12:35,590 --> 00:12:38,870
+اللي نتجت من الـ glycolysis لازم تدخل الميتوكوندريا
+
+172
+00:12:38,870 --> 00:12:42,510
+عشان تحوّلها لـ ATP. كيف بده تدخل؟ من shuttle، من
+
+173
+00:12:42,510 --> 00:12:46,450
+قناة، في عندنا قناتين لدخول الـ NADH، في قناة
+
+174
+00:12:46,450 --> 00:12:51,170
+اسمها malate aspartate shuttle. إذا دخل منها الـ NADH،
+
+175
+00:12:51,170 --> 00:12:55,230
+بعتبره عن ثلاثة ATP. فالاثنين هدول بدرومهم في ثلاثة
+
+176
+00:12:55,230 --> 00:12:59,390
+وبيعطيني ست
+
+201
+00:14:44,200 --> 00:14:48,580
+يتعامل مع أكسجين و هيدروجين أيون تشكل موتر تبدأ
+
+202
+00:14:48,580 --> 00:14:52,200
+الـ H+ تنتقل من سايتو كروم إلى الآخر وصولا
+
+203
+00:14:52,200 --> 00:14:56,500
+للمستقبل النهائي للالكترونات اللي هو الـ oxygen 
+
+204
+00:14:56,500 --> 00:15:00,540
+Last Electron Acceptor اللي هو الأكسجين بيتحد الـ
+
+205
+00:15:00,540 --> 00:15:05,360
+H+ مع الـ O2 بيعطيني H2O فبينتج عن الـ Electron
+
+206
+00:15:05,360 --> 00:15:09,080
+Transport Chain مية H2O
+
+207
+00:15:17,380 --> 00:15:21,960
+مجرد أن ينتقل الـ H+ في الـ cytochrome تنتقل
+
+208
+00:15:21,960 --> 00:15:27,560
+مع NADH و FADH وتحويلها إلى طاقة شكل ATP
+
+209
+00:15:27,560 --> 00:15:33,490
+وتخزينها لحاجة الجسم. نقطة من 1 ل 4 هدول بدنا نشيلها
+
+210
+00:15:33,490 --> 00:15:36,730
+النقطة الخامسة بتحكي ليه أن ال electron transport
+
+211
+00:15:36,730 --> 00:15:40,170
+chain generates no ATP directly أن ال electron
+
+212
+00:15:40,170 --> 00:15:46,340
+transport chain بيمتجش عنها طاقة بشكل مباشر. هنا شكل
+
+213
+00:15:46,340 --> 00:15:50,820
+كأنه شكل للـ Electron Transport Chain أنه بتكون
+
+214
+00:15:50,820 --> 00:15:54,460
+عبارة عن دوائر Cytochrome بينتقل فيها الـ H+
+
+215
+00:15:54,460 --> 00:15:57,760
+من دائرة لـدائرة من الـ Cytochrome للآخر وصولا
+
+216
+00:15:57,760 --> 00:16:00,960
+لآخر أشياء الحمراء اللي تحت اللي هي الـ Oxygen اللي
+
+217
+00:16:00,960 --> 00:16:03,700
+بنعتبرها الـ last electron acceptor والمستقبل
+
+218
+00:16:03,700 --> 00:16:08,440
+النهائي للإلكترونات. تحت معها الـ H+ وبيعطيني مية
+
+219
+00:16:08,440 --> 00:16:14,910
+H2O. الـ fermentation اللي هي anaerobic respiration
+
+220
+00:16:14,910 --> 00:16:19,290
+أنه إذا مش موجود أكسجين الجسم بعد خطوة الـ
+
+221
+00:16:19,290 --> 00:16:22,570
+glycolysis بدي يعمل fermentation anaerobic
+
+222
+00:16:22,570 --> 00:16:26,370
+respiration في الخميرة بنسميها الكحولية
+
+223
+00:16:26,370 --> 00:16:30,590
+fermentation ليش؟ لأنه حكينا أنه النتج النهائي بعد
+
+224
+00:16:30,590 --> 00:16:35,970
+الـ fermentation بينتج الكحول أو الإيثانول. The rate of
+
+225
+00:16:35,970 --> 00:16:38,830
+fermentation can be affected by several factors.
+
+226
+00:16:38,830 --> 00:16:45,930
+معدل التخمر يتأثر بعدد عوامل. معدل التخمر يتأثر بعدد
+
+227
+00:16:45,930 --> 00:16:52,050
+عوامل. أول عامل تركيز الخميرة concentration of yeast
+
+228
+00:16:52,400 --> 00:16:56,000
+تركيز الخميرة بيأثر على معدل الـ fermentation لأن
+
+229
+00:16:56,000 --> 00:17:00,040
+الكائن الحي هذا هو المسؤول عن التنفس. إذا كانت
+
+230
+00:17:00,040 --> 00:17:04,580
+موجودة وتتنفس كمية أكبر. كل ما زادت كمية الخميرة
+
+231
+00:17:04,580 --> 00:17:08,440
+هيزيد معدل التنفس، كل كائن حي بده يعمل تنفس، فكل ما 
+
+232
+00:17:08,440 --> 00:17:12,640
+زادت هيزيد التنفس. concentration of glucose تركيز
+
+233
+00:17:12,640 --> 00:17:17,990
+السكر، مش هيبدأ التنفس علشان تكسر وتنتج الطاقة. فكل ما
+
+234
+00:17:17,990 --> 00:17:22,710
+زاد السكر هيزيد عملية التنفس عشان تنتج الطاقة
+
+235
+00:17:22,710 --> 00:17:28,670
+وكذلك درجة الحرارة بتأثر عليه. For the yeast 
+
+236
+00:17:28,670 --> 00:17:31,010
+cell, this chemical reaction is necessary to 
+
+237
+00:17:31,010 --> 00:17:34,350
+produce energy for life. بالنسبة للخميرة هذه
+
+238
+00:17:34,350 --> 00:17:37,150
+العملية اللي هي fermentation أو الـ cellular respiration
+
+239
+00:17:37,150 --> 00:17:41,530
+علشان التنفس هي ضرورية لإنتاج الطاقة. الكحول
+
+240
+00:17:41,530 --> 00:17:45,130
+و ثاني أكسيد الكربون مصدرها من خلال عملية الهضم
+
+241
+00:17:45,130 --> 00:17:51,670
+بينتج. ناتج العملية بيكون عندنا كحول و CO2. The
+
+242
+00:17:51,670 --> 00:17:54,370
+chemical reaction known as fermentation هي العملية
+
+243
+00:17:54,370 --> 00:17:58,070
+مسميها fermentation. كيف بدنا نقيسها؟ Watched and 
+
+244
+00:17:58,070 --> 00:18:01,250
+measured by the amount of carbon dioxide gas that
+
+245
+00:18:01,250 --> 00:18:06,050
+is produced. من كمية الـ CO2 الناتجة بعرف قداش كمية
+
+246
+00:18:06,050 --> 00:18:10,770
+التنفس الخلوي الناتجة من تكسير الجلوكوز. الـ yeast 
+
+247
+00:18:10,770 --> 00:18:15,090
+اللي هي الخميرة بتعيش في وجود الأكسجين 
+
+248
+00:18:15,090 --> 00:18:19,670
+وفي عدم وجود الأكسجين. إذا الـ yeast بتعيش في ظروف
+
+249
+00:18:19,670 --> 00:18:23,750
+هوائية أو لا هوائية. زي ما حكينا إنها facultative
+
+250
+00:18:23,750 --> 00:18:28,930
+anaerobic. In the presence of oxygen إذا الخميرة
+
+251
+00:18:28,930 --> 00:18:33,560
+كانت متوفرة عندها Oxygen ماذا ستفعل؟ ستفعل Aerobic
+
+252
+00:18:33,560 --> 00:18:38,680
+Respiration. هي تبدأ بالـ Glycolysis ثم بالـ Glycolysis ثم
+
+253
+00:18:38,680 --> 00:18:40,700
+بالـ Glycolysis ثم بالـ Glycolysis ثم بالـ Glycolysis ثم
+
+254
+00:18:40,700 --> 00:18:42,040
+بالـ Glycolysis ثم بالـ Glycolysis ثم بالـ Glycolysis ثم
+
+255
+00:18:42,040 --> 00:18:42,260
+بالـ Glycolysis ثم بالـ Glycolysis ثم بالـ Glycolysis ثم
+
+256
+00:18:42,260 --> 00:18:43,680
+بالـ Glycolysis ثم بالـ Glycolysis ثم بالـ Glycolysis ثم
+
+257
+00:18:43,680 --> 00:18:48,560
+بالـ Glycolysis ثم بالـ Glycolysis ثم بالـ Glycolysis ثم
+
+258
+00:18:48,560 --> 00:18:52,660
+بالـ Glycolysis ثم بالـ Glycolysis ثم بالـ Glycolysis ثم
+
+259
+00:18:52,660 --> 00:18:57,470
+بالـ Glycolysis ثم بالـ Glycolysis ثم لو الأكسجين ليس موجود
+
+260
+00:18:57,470 --> 00:19:02,750
+لكن Yeast فقط تحصل على طاقة من Glycolysis وبعدها 
+
+261
+00:19:02,750 --> 00:19:06,930
+تحول إلى إيثانول. إن هي عملية fermentation إذا لم 
+
+262
+00:19:06,930 --> 00:19:10,690
+تكن موجودة ستبدأ Glycolysis وبعدها سيكون هناك 
+
+263
+00:19:10,690 --> 00:19:14,960
+fermentation وإنتاج للكحول للإيثانول. بحكي له إن هذه
+
+264
+00:19:14,960 --> 00:19:18,160
+less efficient than aerobic respiration هي أقل
+
+265
+00:19:18,160 --> 00:19:22,980
+كفاءة من الـ aerobic respiration. الـ anaerobic أقل
+
+266
+00:19:22,980 --> 00:19:26,600
+كفاءة. كمية الطاقة اللي بتنتج منها أقل. The main
+
+267
+00:19:26,600 --> 00:19:30,620
+source of carbon and energy is glucose. مصدر الطاقة
+
+268
+00:19:30,620 --> 00:19:34,160
+الرئيسي منه وحكينا الجلوكوز بتم في البداية تكسير
+
+269
+00:19:34,160 --> 00:19:40,550
+الجلوكوز تمام؟ لو الجلوكوز موجود بتركيز عالي بيصير
+
+270
+00:19:40,550 --> 00:19:46,370
+عندنا إشارة لخلايا أنه تبدأ عملية respiration عشان
+
+271
+00:19:46,370 --> 00:19:50,910
+تكسر الجلوكوز وتنتج الطاقة. بيحكي للخميرة ممكن
+
+272
+00:19:50,910 --> 00:19:54,250
+تستخدم سكر ثاني غير الجلوكوز مش بس الجلوكوز عندها 
+
+273
+00:19:54,250 --> 00:19:58,850
+مصدر طاقة. ممكن السكروز تحوله لأن السكروز هو سكر
+
+274
+00:19:58,850 --> 00:20:03,090
+ثنائي عبارة عن جلوكوز وفركتوز. في عندها إنزيم
+
+275
+00:20:03,090 --> 00:20:10,180
+اسمه Invertase. الإنزيم الانفرتيز بيكسر السكروز إلى 
+
+276
+00:20:10,180 --> 00:20:15,260
+جلوكوز وفركتوز. إنزيم الانفرتيز بيكسر السكروز إلى
+
+277
+00:20:15,260 --> 00:20:19,320
+جلوكوز وفركتوز. بتقدر تاخد الجلوكوز و تستخدمه في
+
+278
+00:20:19,320 --> 00:20:23,360
+إنتاج الطاقة. كمان سكر ثاني ممكن تستخدمه الخميرة
+
+279
+00:20:23,360 --> 00:20:28,350
+اللي هو المالتوز. أنه عندها إنزيم اسمه Maltase بيكسر
+
+280
+00:20:28,350 --> 00:20:31,950
+المالتوز إلى جلوكوز وجلوكوز. لأن احنا عارفين إن
+
+281
+00:20:31,950 --> 00:20:36,270
+المالتوز هو سكر ثنائي عبارة عن اتحاد جلوكوز مع 
+
+282
+00:20:36,270 --> 00:20:41,050
+جلوكوز. فبتكسر. الإنزيم المالتيز بيكسر المالتوز إلى 
+
+283
+00:20:41,050 --> 00:20:46,790
+جلوكوز وجلوكوز بتستخدمهم في إنتاج الطاقة. هنا عندنا
+
+284
+00:20:46,790 --> 00:20:51,950
+مخطط إن الخميرة إيش هتمشي بالبداية سواء في أكسجين
+
+285
+00:20:51,950 --> 00:20:55,890
+أو فيش أكسجين. فوق هتستهل اللي هو فوق هي مكتوب إنه
+
+286
+00:20:55,890 --> 00:20:59,490
+هتاخد الجلوكوز تعمل عليها عملية الـ glycolysis
+
+287
+00:20:59,490 --> 00:21:04,490
+هتعطيني اتنين بيروفيت تمام؟ اتنين بيروفيت هدول بعدك
+
+288
+00:21:04,490 --> 00:21:08,710
+إذا الخميرة عندها أكسجين هتروح على جهة اليسار اللي 
+
+289
+00:21:08,710 --> 00:21:13,630
+هي anaerobic aerobic respiration اللي هو تنفس هوائي
+
+290
+00:21:14,300 --> 00:21:17,800
+اليومين. إذا الخميرة ما فيش عندها أكسجين الـ
+
+291
+00:21:17,800 --> 00:21:22,900
+pyruvate هتهيأ يلجأ إلى anaerobic respiration اللي
+
+292
+00:21:22,900 --> 00:21:28,420
+هي إنتاج الـ Ethanol و الكحول. في ما عملنا اليوم
+
+293
+00:21:28,420 --> 00:21:33,200
+نجيب خميرة و نجيب سكر. نحطهم مع بعض و ندوبهم في شوية
+
+294
+00:21:33,200 --> 00:21:38,020
+ماء و نشوف قداش معدل التنفس الخلوي اللي نتج من
+
+295
+00:21:38,020 --> 00:21:42,940
+خلال قياس كمية الـ CO2 اللي نتجت. فاحنا بدنا نعمل
+
+296
+00:21:42,940 --> 00:21:47,140
+study of cellular respiration in yeast. إيش الهدف
+
+297
+00:21:47,140 --> 00:21:51,460
+من ما عملنا study cellular respiration in yeast by
+
+298
+00:21:51,460 --> 00:21:56,600
+measuring the amount of CO2 produced. من خلال قياس 
+
+299
+00:21:56,600 --> 00:22:01,260
+كمية الـ CO2 اللي بتنتج. بنستخدم جهاز اسمه
+
+300
+00:22:01,260 --> 00:22:05,600
+Respirometer. الـ Respirometer هي أداة device to
+
+301
+00:22:05,600 --> 00:22:08,420
+measure the rate of respiration of living organism
+
+302
+00:22:08,420 --> 00:22:11,580
+by measuring the rate of the exchange of oxygen 
+
+303
+00:22:11,580 --> 00:22:16,480
+and/or carbon dioxide. هي أداة بنستخدمها لقياس معدل
+
+304
+00:22:16,480 --> 00:22:21,520
+التنفس الخلوي. الـ Spirometer أداة هنستخدمها لقياس
+
+305
+00:22:21,520 --> 00:22:26,980
+معدل التنفس الخلوي من خلال قياس كمية الـ Oxygen أو 
+
+306
+00:22:26,980 --> 00:22:30,980
+الـ CO2 الناتجة. هذه الأداة هنتعرف عليها وهوريكوا 
+
+307
+00:22:30,980 --> 00:22:35,260
+إياها في المعمل. بدنا محلول سكر شجر جلوكوز طبيعي 
+
+308
+00:22:35,260 --> 00:22:39,840
+بدنا سكر و بدنا Yeast الكائن الحي أحط له سكر عشان
+
+309
+00:22:39,840 --> 00:22:44,020
+يستهلك السكر هذا وينتج الطاقة وبده ماء أدوبهم فيه
+
+310
+00:22:45,000 --> 00:22:49,540
+خطوات العمل. هذا الجدول ناقص وأنا هصور لكم إياه
+
+311
+00:22:49,540 --> 00:22:54,560
+خطوات العمل إن أنا بدي أجيب خمسة أنابيب تمام؟ بدي
+
+312
+00:22:54,560 --> 00:22:59,300
+أجيب خمسة أنابيب. الخمسة أنابيب هدول بدي أرقمهم من
+
+313
+00:22:59,300 --> 00:23:03,060
+واحد لخمسة وأشتغل عليهم. إيش بدي أعمل؟ أنبوب رقم 
+
+314
+00:23:03,060 --> 00:23:07,560
+واحد بدي أحط عليها أربعة مل من الماء و صفر من
+
+315
+00:23:07,560 --> 00:23:10,820
+الخميرة. بديش أحط عليها خميرة خالص وبده أحط عليها
+
+316
+00:23:10,820 --> 00:23:15,370
+ثلاثة مل من السكر. الأنبوب رقم واحد أنا ما حطيتش خميرة 
+
+317
+00:23:15,370 --> 00:23:18,630
+بس حطيت سكر مع ماء. هل هيصير فيها cellular
+
+318
+00:23:18,630 --> 00:23:23,570
+respiration؟ أكيد مش هيصير فيها ومش هينتج عنه CO2 
+
+319
+00:23:23,570 --> 00:23:27,930
+ولا أكسجين. مش هشوف CO2 ناتج ليش؟ لأنه فيش الكائن
+
+320
+00:23:27,930 --> 00:23:30,970
+الحي اللي بديعمل لي عملية التنفس الخلوي. الـ Yeast مش
+
+321
+00:23:30,970 --> 00:23:35,370
+موجودة. أنا ما ضفتش Yeast. بأنبوب رقم اثنين بدي أحط
+
+322
+00:23:35,370 --> 00:23:40,590
+ستة مل من المياه على واحد مل من الـ Yeast و صفر مل
+
+323
+00:23:40,590 --> 00:23:45,050
+من السكر. بديش أحط سكر. طب أنا ما حطيتش سكر، بس حطيت 
+
+324
+00:23:45,050 --> 00:23:47,990
+خميرة ومع الماء. هل هيصير فيها برضه cellular 
+
+325
+00:23:47,990 --> 00:23:53,190
+respiration؟ لأ برضه مش هيصير، ليش؟ لأنه الكائن
+
+326
+00:23:53,190 --> 00:23:57,630
+الحي موجود، بس مصدر الطاقة اللي هو السكر مش موجود، 
+
+327
+00:23:57,630 --> 00:24:00,530
+أنا ما حطيتش سكر، طب إيش هي بدها تستخدم عشان تعمل
+
+328
+00:24:00,530 --> 00:24:03,630
+.. تنتج الطاقة؟ فمش هيصير فيها برضه cellular
+
+329
+00:24:03,630 --> 00:24:08,710
+respiration. طبعا أنبوب واحد و اثنين عاملهم ك
+
+330
+00:24:08,710 --> 00:24:13,050
+negative control كcontrol عشان أشوف إن أنا اشتغل
+
+331
+00:24:13,050 --> 00:24:16,770
+الصح ولا لا. المفروض أنبوب واحد و اثنين ما يطلعش فيها
+
+332
+00:24:16,770 --> 00:24:20,710
+CO2. و الأنبوب رقم ثلاثة و أربعة و خمسة هم اللي بدنا 
+
+333
+00:24:20,710 --> 00:24:24,310
+نشتغل عليهم. إيش بدنا نحط على أنبوب رقم ثلاثة؟ بدنا نحط
+
+334
+00:24:24,310 --> 00:24:30,170
+عليها ثلاثة مل من ماء على واحد مل من الخميرة على 3 
+
+335
+00:24:30,170 --> 00:24:35,450
+مل سكر، ده حاط 3 مل من الماء مع 1 مل من الخميرة
+
+336
+00:24:35,450 --> 00:24:39,270
+على 3 مل من السكر، بشوف كمية التنفس اللي بتصير 
+
+337
+00:24:39,270 --> 00:24:44,410
+عندي، الـ CO2 اللي بتطلع. أنبوب رقم 4 بدي أضع عليها 1
+
+338
+00:24:44,410 --> 00:24:50,450
+مل من الماء على 3 مل من الخميرة على 3 مل من السكر
+
+339
+00:24:50,450 --> 00:24:55,910
+وأشوف كمية الـ CO2. أنبوب رقم 5 بدي أضع عليها 2 مل من 
+
+340
+00:24:55,910 --> 00:25:02,910
+الماء على 2 مل من الخميرة على 3 مل من السكر. يعني 
+
+341
+00:25:02,910 --> 00:25:07,230
+أنا في هذه الخطوات في أنبوب رقم 3 أو 4 أو 5 ثبتت
+
+342
+00:25:07,230 --> 00:25:11,830
+كمية السكر في التلات قناني. حطيت 3 مل من السكر كنت
+
+343
+00:25:11,830 --> 00:25:17,630
+أغير كمية الخميرة حطيت 1 مل على أنبوب رقم 3 و 3 مل
+
+344
+00:25:17,630 --> 00:25:22,990
+على أنبوب رقم 4 و 2 مل على أنبوب رقم 5. فالسكر مش
+
+345
+00:25:22,990 --> 00:25:27,850
+هيأثر. مين اللي بيأثر هنا؟ كمية الخميرة كل ما تزيد 
+
+346
+00:25:27,850 --> 00:25:32,430
+الكائن الحي الموجود هيزيد معدل التنفس الخلوي. فأي
+
+347
+00:25:32,430 --> 00:25:36,490
+أنبوب هنتوقع أن يكون فيها تنفس خلوي أكثر؟ أكيد
+
+348
+00:25:36,490 --> 00:25:40,450
+الأنبوب رقم أربعة لأنه بتحتوي على خميرة أكثر. بتحتوي
+
+349
+00:25:40,450 --> 00:25:44,290
+على ثلاثة مل. أما أنبوب ثلاثة بتحتوي بس على واحد مل
+
+350
+00:25:44,290 --> 00:25:49,130
+أنبوب خمسة بتحتوي على اثنين مل. أنبوب رقم 4 هي أكثر 
+
+351
+00:25:49,130 --> 00:25:52,810
+الكائن الحي موجود فيها بكمية أكبر. الـ Yeast موجودة
+
+352
+00:25:52,810 --> 00:25:58,390
+أكثر then الـ CO2 هتنتج عنها أكبر. إشي هنا خطوات 
+
+353
+00:25:58,390 --> 00:26:02,170
+العمل. بعد ما أعمل في الخمسة أنابيب هدول بجيب جهاز
+
+354
+00:26:02,170 --> 00:26:08,890
+الـ Respirometer. بحطه في هاد في الأنبوب وبسحب
+
+355
+00:26:08,890 --> 00:26:12,990
+السائل بقى أرفعه في هذا الجهاز. طبعا هنا مش هتفهموا
+
+356
+00:26:12,990 --> 00:26:15,790
+كيف إنه هذا الجهاز لما ناخده في المعمل هوريكوا
+
+357
+00:26:15,790 --> 00:26:20,330
+الجهاز وكيف هنحط فيه المحلول وبعدين لكي اللي 
+
+358
+00:26:20,330 --> 00:26:23,810
+عملنا الجدول اللي قبل وبعدين بأخد هذا الجهاز و
+
+359
+00:26:23,810 --> 00:26:28,630
+بحطه في الـ spectrophotometer بحطه في الـ water bath 
+
+360
+00:26:28,630 --> 00:26:32,950
+بحطه على درجة حرارة ثابتة عشان ما يؤثرش وبشوف كمية 
+
+361
+00:26:32,950 --> 00:26:36,790
+الـ CO2 اللي بتنتج. هذا هنشوفه في معمل الجهاز عشان 
+
+362
+00:26:36,790 --> 00:26:41,630
+نفهم أكثر وهيك بنكون خلصنا ويعطيكم العافية 
diff --git a/PL9fwy3NUQKwZ1Q7zOg1ObgufPMjRI3WY1/h8ODbKj_UcE_postprocess.srt b/PL9fwy3NUQKwZ1Q7zOg1ObgufPMjRI3WY1/h8ODbKj_UcE_postprocess.srt
new file mode 100644
index 0000000000000000000000000000000000000000..5f1ab5bea49e892b7403ed12ec6e95d7da445fd8
--- /dev/null
+++ b/PL9fwy3NUQKwZ1Q7zOg1ObgufPMjRI3WY1/h8ODbKj_UcE_postprocess.srt
@@ -0,0 +1,1448 @@
+1
+00:00:00,000 --> 00:00:04,700
+نحكي في آخر معمل عنا اللي هو عبارة عن معمل energy
+
+2
+00:00:04,700 --> 00:00:09,080
+metabolism in yeast تفاعلات إنتاج الطاقة في
+
+3
+00:00:09,080 --> 00:00:12,900
+الكائنات الحية والكائن الحي اللي هنحكي عنه اليوم
+
+4
+00:00:12,900 --> 00:00:18,510
+هو الخميرةأولا نحكي عن الـ Cellular Respiration
+
+5
+00:00:18,510 --> 00:00:22,690
+اللي هو التنفس الخلوي إيش هو التنفس الخلوي؟ هو set
+
+6
+00:00:22,690 --> 00:00:26,170
+of metabolic reactions and processes that take
+
+7
+00:00:26,170 --> 00:00:29,330
+place in the cell of organisms to convert
+
+8
+00:00:29,330 --> 00:00:34,150
+biochemical energy from nutrients into ATP مجموعة
+
+9
+00:00:34,150 --> 00:00:38,550
+من التفاعلات الحيوية في داخل خلايا الكائنات الحية
+
+10
+00:00:38,550 --> 00:00:45,280
+للحصول على الطاقةأو إنتاج الطاقة على شكل ATP من
+
+11
+00:00:45,280 --> 00:00:49,960
+الطعام اللي بنتناوله إذا هي تفاعلات حيوية في داخل
+
+12
+00:00:49,960 --> 00:00:54,980
+خلايا الجينات الحية لإنتاج الطاقة على شكل ATP من
+
+13
+00:00:54,980 --> 00:01:00,400
+الطعام اللي بنتناولهاتفاعلات إنتاج الطاقة تعتبر
+
+14
+00:01:00,400 --> 00:01:05,980
+catabolic reaction، تفاعلات هدم وليس تفاعلات بناء،
+
+15
+00:01:05,980 --> 00:01:09,180
+break large molecule into smaller one releasing
+
+16
+00:01:09,180 --> 00:01:14,180
+energy عملية تكسير، مركبة كبيرة إلى صغيرة بينتج
+
+17
+00:01:14,180 --> 00:01:19,120
+عنها طاقة نحكي عن الكهن الحي اللي بندرسه اليوم
+
+18
+00:01:19,120 --> 00:01:21,880
+اللي هو ال yeast اسمها العلمي Saccharomyces
+
+19
+00:01:21,880 --> 00:01:26,420
+cerevisiaاليست هي living organisms that require a
+
+20
+00:01:26,420 --> 00:01:29,660
+warm, moist environment and a food source to grow
+
+21
+00:01:29,660 --> 00:01:34,100
+and thrive هي الكائن حي بيحتاج إلى بيئة دافعة و
+
+22
+00:01:34,100 --> 00:01:41,100
+راطبة و يحتاج إلى مصدر طعام لنمو و المعيشة عشان
+
+23
+00:01:41,100 --> 00:01:46,140
+تنمو و تعيش بدها بيئة راطبة تكون و دافعة و بدها
+
+24
+00:01:46,140 --> 00:01:52,060
+مصدر غذاء هي unicellular أحدية الخليةsingle cell
+
+25
+00:01:52,060 --> 00:01:56,920
+facultative anaerobic fungi هي خلايا بتعيش في وجود
+
+26
+00:01:56,920 --> 00:02:00,860
+هي كائنات حية تعيش في وجود ال oxygen أو عدم وجود
+
+27
+00:02:00,860 --> 00:02:04,920
+ال oxygen هي اختيارية تعيش في وجود ال oxygen أو
+
+28
+00:02:04,920 --> 00:02:09,880
+عدم وجود ال oxygen تحت ال microscope كيف شكلها
+
+29
+00:02:09,880 --> 00:02:14,380
+بيكون بيكون شكلها oval بيداوي وإذا كانت بتنقسم
+
+30
+00:02:14,380 --> 00:02:21,150
+بتكون مطلعة تبرع بضمcellular respiration بيحكي
+
+31
+00:02:21,150 --> 00:02:24,450
+لإنه في هذا المعمل will be using yeast to measure
+
+32
+00:02:24,450 --> 00:02:27,770
+the rate of cellular respiration لنستخدم الخميرة
+
+33
+00:02:27,770 --> 00:02:32,050
+عشان نقيس معدل التنفس الخلوي عندها كدهش معدل
+
+34
+00:02:32,050 --> 00:02:37,010
+التنفس الخلوي عند الخميرةالغميرة هي single cell هي
+
+35
+00:02:37,010 --> 00:02:41,590
+حكينا انها حدية الخلية ايو كاريوتك organism حقيقة
+
+36
+00:02:41,590 --> 00:02:46,410
+انها تستهلك الكاربوهيدرات السكريات عشان تنتج ال
+
+37
+00:02:46,410 --> 00:02:50,810
+ATP نفس الطريقة زيها زي النبات والحيوان
+
+38
+00:03:00,090 --> 00:03:04,710
+الخميرة بتحول السكر لمية و Carbon Dioxide عشان
+
+39
+00:03:04,710 --> 00:03:09,730
+تحصل على الطاقة إذا السكر بيدخل و بينتج عنه by
+
+40
+00:03:09,730 --> 00:03:12,610
+product من الcellular respiration بينتج عنه مية
+
+41
+00:03:12,610 --> 00:03:19,100
+water H2O و CO2 و Carbon Dioxide و ATP طاقةThe
+
+42
+00:03:19,100 --> 00:03:21,760
+greater the carbon dioxide production, the greater
+
+43
+00:03:21,760 --> 00:03:25,460
+the rate of cellular respiration كل ما كانت كمية
+
+44
+00:03:25,460 --> 00:03:30,260
+الـ CO2 ناتجة أكبر، كمية أكبر، إذا المعدل التنفسي
+
+45
+00:03:30,260 --> 00:03:35,450
+الخلوي اللي صار عندي أكبرإيش مراحل ال cellular
+
+46
+00:03:35,450 --> 00:03:39,590
+respiration stage of cellular respiration أن تلت
+
+47
+00:03:39,590 --> 00:03:43,750
+مراحل أول مرحلة ال glycolysis اللي بتحدث في ال
+
+48
+00:03:43,750 --> 00:03:48,050
+cytosol أول مرحلة glycolysis في ال cytosol تاني
+
+49
+00:03:48,050 --> 00:03:51,430
+مرحلة ال Krebs Cycle اللي تحدث في ال mitochondrial
+
+50
+00:03:51,430 --> 00:03:56,390
+matrix تالت مرحلة electron transport chain تحدث في
+
+51
+00:03:56,390 --> 00:04:01,190
+inner membrane of mitochondria لذا ال glycolysis
+
+52
+00:04:01,190 --> 00:04:06,070
+في ال cytosol Krebs Cycleالـ mitochondrial matrix
+
+53
+00:04:06,070 --> 00:04:11,030
+التجويف الداخلي للميتوكندرية electron transported
+
+54
+00:04:11,030 --> 00:04:17,420
+chain تحدث في ال membrane الداخلي للميتوكندريةأول
+
+55
+00:04:17,420 --> 00:04:21,100
+مرحلة ال glycolysis هي عملية بتحدث في ال cytosol
+
+56
+00:04:21,100 --> 00:04:24,960
+زي ما حكينا بتحدث في وجود أو عدم وجود ال oxygen
+
+57
+00:04:24,960 --> 00:04:28,080
+with or without the presence of oxygen يعني إذا
+
+58
+00:04:28,080 --> 00:04:32,460
+الجسم محتاج لطاقة إذا في oxygen أو فش oxygen أول
+
+59
+00:04:32,460 --> 00:04:35,980
+مرحلة لازم يبدأها هي ال glycolysis إيش هذه
+
+60
+00:04:35,980 --> 00:04:40,140
+العملية؟ تم تحويل one molecule of glucose into two
+
+61
+00:04:40,140 --> 00:04:45,840
+molecules of pyruvateإنه يكسر سكر الجليكوز وينتج
+
+62
+00:04:45,840 --> 00:04:50,280
+عنه اتنين بيروفات هذه العملية بينتج عنها تونة
+
+63
+00:04:50,280 --> 00:04:55,860
+molecule of ATP بينتج عنها كام محصلة اتنين ATP إذا
+
+64
+00:04:55,860 --> 00:04:59,560
+عملية الـGlycolysis تكسير الجليكوز إلى اتنين
+
+65
+00:04:59,560 --> 00:05:04,640
+بيروفات بينتج عنها في الحقيقة بينتج اربعة ATP ولكن
+
+66
+00:05:04,640 --> 00:05:07,980
+هذه العملية بتستهلك منهم اتنين وبيظل عنده اتنين
+
+67
+00:05:08,560 --> 00:05:12,840
+فهما four molecules of ATP are reproduced ولكن ال
+
+68
+00:05:12,840 --> 00:05:17,080
+net number ال number of ATP جدش العدد هو أربع لكن
+
+69
+00:05:17,080 --> 00:05:19,980
+ال net number الحصل النهائي اللي طلعنا منها من
+
+70
+00:05:19,980 --> 00:05:21,960
+الطاقة هي اتنين ATP
+
+71
+00:05:25,180 --> 00:05:28,420
+the fate of pyruvate هلجت بعد هذه العملية بعد
+
+72
+00:05:28,420 --> 00:05:31,940
+الخطوة الأولى اللى هى ال glycolysis اللى حدثت فيه
+
+73
+00:05:31,940 --> 00:05:35,740
+وجود أو عدم وجود ال oxygen ال pyruvate اللى نتج
+
+74
+00:05:35,740 --> 00:05:41,550
+ايش مصيره مصير ال pyruvateالبيروفيت هذا له مصيرين
+
+75
+00:05:41,550 --> 00:05:45,890
+إذا في حالة وجود الأكسجين بيروح على جهة في حالة
+
+76
+00:05:45,890 --> 00:05:49,710
+عدم وجود الأكسجين بيبدأ حاجة تانية إذا الأكسجين
+
+77
+00:05:49,710 --> 00:05:54,990
+موجود هيدخل هذا البيروفيت على الميتوكندرية يتحول
+
+78
+00:05:54,990 --> 00:05:59,330
+إلى أسيت الكو إيه الأسيت الكو إيه هذه بتدخل على
+
+79
+00:05:59,330 --> 00:06:03,190
+كريبس سايكل وبعدين خطوة تالتة اللي هي ال electron
+
+80
+00:06:03,190 --> 00:06:08,180
+transport chainأول عملية جليكوليسية تحدث في وجود
+
+81
+00:06:08,180 --> 00:06:12,440
+أو عند موجود الأكسجين بيمتج عنها بيروفين بيروفيتها
+
+82
+00:06:12,440 --> 00:06:17,680
+إذا في أكسجين هيكمل aerobic respiration تنفس خلوي
+
+83
+00:06:17,680 --> 00:06:22,640
+هو aerobic respiration اللي هي كربس سايكل و بعدين
+
+84
+00:06:22,640 --> 00:06:27,040
+الكترون تنفس بورتتين إذا فاش أكسجين هيبدأ عملية
+
+85
+00:06:27,040 --> 00:06:31,340
+اسمها fermentation أو anaerobic respiration نسميها
+
+86
+00:06:31,340 --> 00:06:36,100
+fermentation أو anaerobic respirationفي حالة
+
+87
+00:06:36,100 --> 00:06:39,440
+الحيوان والبلاند والنبات في الـ Anaerobic
+
+88
+00:06:39,440 --> 00:06:43,140
+respiration ينتج عنها لاكتك أسد الناتج النهائي
+
+89
+00:06:43,140 --> 00:06:47,520
+لاكتك أسد في حالة اليست والبكتيريا الناتج النهائي
+
+90
+00:06:47,520 --> 00:06:51,660
+هو إيتانول هو كحول إيتانول هذا إذا عدم وجود
+
+91
+00:06:51,660 --> 00:06:55,440
+الأكسجين fermentation في الانمال والبلاند بينتج
+
+92
+00:06:55,440 --> 00:06:58,900
+لاكتك أسد في اليست والبكتيريا بينتج إيتانول أو
+
+93
+00:06:58,900 --> 00:07:04,440
+كحولفعشان هيك بنفسر انه ليش لما تبدأ المجهود عند
+
+94
+00:07:04,440 --> 00:07:07,820
+بدء المجهود بتتعب العضلة ليش؟ لأنه مافيش أكسجين
+
+95
+00:07:07,820 --> 00:07:12,100
+بيوصل للعضلة فالعضلة هتبدأ لعملية fermentation لأن
+
+96
+00:07:12,100 --> 00:07:15,420
+مافيش أكسجين همش هتعمل ال aerobic respiration
+
+97
+00:07:15,420 --> 00:07:18,340
+هتعمل anaerobic respiration اللي هي fermentation
+
+98
+00:07:18,340 --> 00:07:22,900
+وهي ال fermentation بينتج عنها lactic acid بيتراكم
+
+99
+00:07:22,900 --> 00:07:27,480
+في العضلات فبتتعب العضلةهنا مكتوب أن الـ fat of
+
+100
+00:07:27,480 --> 00:07:30,380
+pyruvate مصير الـ pyruvate بيحكي اللي بيدخل الـ
+
+101
+00:07:30,380 --> 00:07:34,440
+mitochondria بيتحول إلى أسيت الكو إيه، الأسيت الكو
+
+102
+00:07:34,440 --> 00:07:38,200
+إيه هي اللي بتبدأ الـ Krebs Cycle بس المركب اللي
+
+103
+00:07:38,200 --> 00:07:41,820
+بيبدأ ال Krebs Cycle هو الأسيت الكو إيه هي اللي
+
+104
+00:07:41,820 --> 00:07:45,380
+بتبدأ ال Krebs Cycle مش ال pyruvate نقطة اتنين و
+
+105
+00:07:45,380 --> 00:07:48,990
+تلاتة بدنا شيهاحكيلي وين ال oxygen is present إذا
+
+106
+00:07:48,990 --> 00:07:52,590
+ال oxygen موجود هيبدأ aerobic respiration اللي هي
+
+107
+00:07:52,590 --> 00:07:56,110
+creeps cycle و بعدين electron transport chain لو
+
+108
+00:07:56,110 --> 00:07:58,870
+ال oxygen not present مش موجود هيصير عندي
+
+109
+00:07:58,870 --> 00:08:02,450
+fermentation ل ال pyruvate هيبدأ anaerobic
+
+110
+00:08:02,450 --> 00:08:07,760
+respiration إذا ال oxygen موجودالاسيت الكو ايه
+
+111
+00:08:07,760 --> 00:08:11,360
+هتحول ال بيروفيت لاسيت الكو ايه اسيت الكو ايه هذا
+
+112
+00:08:11,360 --> 00:08:15,200
+بيبدأ اللي هى ال citric acid cycle ال citric acid
+
+113
+00:08:15,200 --> 00:08:19,620
+cycle هى نفسها ال crib cycle اسم تاني اللى بتحدث
+
+114
+00:08:19,620 --> 00:08:24,100
+فى داخل ال mitochondrial matrix بينتج عنها CO2 و
+
+115
+00:08:24,100 --> 00:08:29,340
+NADH تمام الطاقة اللى بتنتج عنها على شكل NADH ال
+
+116
+00:08:29,340 --> 00:08:33,710
+citric acid cycle هى عبارة عن تمان خطواتهي عبارة
+
+117
+00:08:33,710 --> 00:08:38,490
+عن تمان خطوات تحتاج إلى أنزين و co-enzyme إذا
+
+118
+00:08:38,490 --> 00:08:41,610
+حولها إلى شي حاجة بيصير عندنا glycolysis على
+
+119
+00:08:41,610 --> 00:08:45,090
+اليسار من البداية ال glycolysis بينتج عنها بيروفيت
+
+120
+00:08:45,090 --> 00:08:49,110
+بيروفيت هاد بيدخل على الميتوكندرية بتم تحويله إلى
+
+121
+00:08:49,110 --> 00:08:53,270
+أسيت الكوهين عملية تحويله إلى أسيت الكوهين بينتج
+
+122
+00:08:53,270 --> 00:08:59,250
+عنها CO2 و NADH تمام هذا الأسيت الكوهين هو اللي
+
+123
+00:08:59,250 --> 00:09:05,870
+هيبدأ بـ Krebs Cycleففي عندي اتنين بيروفيات لما
+
+124
+00:09:05,870 --> 00:09:10,970
+تحول لاسد الكو اي نتج عنهم اتنين ناد اش في creeps
+
+125
+00:09:10,970 --> 00:09:15,330
+cycle الاسم التاني حكينا citric acid cycle ويلها
+
+126
+00:09:15,330 --> 00:09:21,590
+اسم تالت تي سي اي تراي كربوكسيليك اسد سايكل اذا ال
+
+127
+00:09:21,590 --> 00:09:25,650
+creeps cycle يلها تلت مسميات creeps cycle, citric
+
+128
+00:09:25,650 --> 00:09:29,610
+acid cycle و تراي كربوكسيليك اسد سايكل تي سي اي
+
+129
+00:09:30,710 --> 00:09:34,290
+تحدث فيه ان الـ Mitochondrial Matrix تحتاج إلى
+
+130
+00:09:34,290 --> 00:09:37,690
+وجود الأكسجين، هذه العملية لا تحدث في عدم وجود
+
+131
+00:09:37,690 --> 00:09:42,030
+الأكسجين، وإنما تحتاج إلى أكسجين، I-Robin في كل
+
+132
+00:09:42,030 --> 00:09:49,320
+واحد جليكوز، بتصير هذه الحلقة مرتينCrip Cycle لكل
+
+133
+00:09:49,320 --> 00:09:53,840
+واحد جليكوز بتصير مرتين عن كل مرة بينتج one ATP
+
+134
+00:09:53,840 --> 00:09:58,220
+تلاتة ندقاش و واحد فادقاش كل حلقة كل دورة بينتج
+
+135
+00:09:58,220 --> 00:10:03,200
+one ATP تلاتة ندقاش و واحد فادقاش طيب لكل واحد
+
+136
+00:10:03,200 --> 00:10:07,480
+جليكوز بتصير مرتين يعني كل واحد جليكوز الناتج
+
+137
+00:10:07,480 --> 00:10:15,000
+هيكون اتنين ATP ستة ندقاش و اتنين فادقاشالنتجة هي
+
+138
+00:10:15,000 --> 00:10:20,240
+انها كريبس سايكل من واحد جيليكوز هو اثنين ATP، ستة
+
+139
+00:10:20,240 --> 00:10:27,180
+ناد أتش واثنين فاد أتشهنا مخطط للعملية ككل أول إشي
+
+140
+00:10:27,180 --> 00:10:31,280
+أن الجليكوز فا الجسم محتاج لطاقة بيبدأ بياخد
+
+141
+00:10:31,280 --> 00:10:34,500
+الجليكوز هاد و بيعمله glycolysis اللي بتحدث في ال
+
+142
+00:10:34,500 --> 00:10:38,020
+cytosol خارج ال mitochondria بيصير ال glycolysis
+
+143
+00:10:38,020 --> 00:10:40,960
+بينتج عنه اتنين بيروفات تحويل الجليكوز لاتنين
+
+144
+00:10:40,960 --> 00:10:44,620
+بيروفات حكينا إنه يتحول لاتنين بيروفات على اليمين
+
+145
+00:10:44,620 --> 00:10:49,620
+جداش بينتج عندي اتنين ATP كمحصلة من تحويل الجليكوز
+
+146
+00:10:49,620 --> 00:10:53,040
+إلى اتنين بيروفات بينتج عندي اتنين ATP و اتنين
+
+147
+00:10:53,040 --> 00:10:56,980
+NADHتمام؟ وجدت الاتنين بيروفيت هاد يدخل
+
+148
+00:10:56,980 --> 00:11:01,380
+الميتوكندريا اللي بدي تم تحويله إلى أست الـKa فتحت
+
+149
+00:11:01,380 --> 00:11:06,980
+البيروفيت في عنا سهمين سهم CO2 وسهم 2 ند اتش هدول
+
+150
+00:11:06,980 --> 00:11:10,700
+من وين أجوا؟ من تحويل البيروفيت إلى أست الـKa
+
+151
+00:11:10,700 --> 00:11:15,440
+فبدنا نكتب تحت البيروفيت أست الـKaبعدين ال acid
+
+152
+00:11:15,440 --> 00:11:19,280
+الكوينيهات بدي يدخل على creeps cycle في ال creeps
+
+153
+00:11:19,280 --> 00:11:23,600
+cycle بتصير عندنا عدة خطوات بينتج عنها اربعة CO2
+
+154
+00:11:23,600 --> 00:11:30,880
+اتنين ATP ستة ناد H و اتنين فاد H2 اللي كانت الناد
+
+155
+00:11:30,880 --> 00:11:34,620
+و الفاد بيدخلوا للنتجين اللي كلهم بيدخلوا على
+
+156
+00:11:34,620 --> 00:11:38,360
+electron transport chain على سلسلة نقل الالكترونات
+
+157
+00:11:38,360 --> 00:11:42,520
+اللي بينتج عنها ميواطربتم تحويل الناد أتش والفاد
+
+158
+00:11:42,520 --> 00:11:48,220
+أتش في هذه السلسلة إلى ATP تمام اجيت هنا عند جدول
+
+159
+00:11:48,220 --> 00:11:52,160
+ملخص للطاقة للناد أتش في عملية ال glycolysis حكينا
+
+160
+00:11:52,160 --> 00:11:56,580
+انه بينتج عنها اتنين ATP كمحصلة و اتنين ناد أتش
+
+161
+00:11:56,580 --> 00:12:00,660
+اذا في ال glycolysis بينتج عنده اتنين ATP و اتنين
+
+162
+00:12:00,660 --> 00:12:04,440
+ناد أتش بتعرفوا ان الناد أتش اذا بدي احوله ل ATP
+
+163
+00:12:04,440 --> 00:12:08,720
+بضربه في تلاتة تمامالناد أتش إذا بدى حوله ل ATP
+
+164
+00:12:08,720 --> 00:12:12,360
+بضربه في تلاتة لكن هذه الناد أتش اللي نتجت في ال
+
+165
+00:12:12,360 --> 00:12:16,440
+glycolysis نتجت في ال cytosol لازم تدخل بالأول على
+
+166
+00:12:16,440 --> 00:12:19,360
+الميتوكندرية و بعدين على ال electrode اسبارتشين
+
+167
+00:12:19,360 --> 00:12:23,200
+عشان تتحول ل ATP هذه الناد أتش كيف ده تدخل ل
+
+168
+00:12:23,200 --> 00:12:27,400
+الميتوكندرية؟ في عندها قناتين في ممرين عشان تدخل
+
+169
+00:12:27,400 --> 00:12:32,250
+الميتوكندرية في ممر بنسميه ملات اسبارتات شطلفي ممر
+
+170
+00:12:32,250 --> 00:12:35,590
+اسمه glycerophosphate shuttle إذا إن الناد أتش
+
+171
+00:12:35,590 --> 00:12:38,870
+اللي نتجته ال glycolysis لازم تدخل الميتوكندريال
+
+172
+00:12:38,870 --> 00:12:42,510
+عشان تحوّلها ل ATB كيف ده تدخل؟ من shuttle، من
+
+173
+00:12:42,510 --> 00:12:46,450
+قناة، في عندنا قناتين لدخول الناد أتش، في قناة
+
+174
+00:12:46,450 --> 00:12:51,170
+اسمها مالية أسبارتية شطر، إذا دخل منها الناد أتش،
+
+175
+00:12:51,170 --> 00:12:55,230
+بعتبره عن تلاتة ATB فالاتنين هدول بدرومهم في تلاتة
+
+176
+00:12:55,230 --> 00:12:59,390
+وبيعطيني ستة ATBإذا دخل النادقاتش من القناة اسمها
+
+177
+00:12:59,390 --> 00:13:04,130
+Glossophosphate Shuttle لأ باعتبر الواحد نادقاتش
+
+178
+00:13:04,130 --> 00:13:08,350
+عن اتنين فبضرب باتنين بتكون اتنين ضرب اتنين بيطلع
+
+179
+00:13:08,350 --> 00:13:11,430
+عندي أربعة فليش كتبلي من أربعة لست ان اتنين
+
+180
+00:13:11,430 --> 00:13:14,210
+نادقاتش هاد لما أحولها ل A to B هيطلع عندي يا
+
+181
+00:13:14,210 --> 00:13:18,490
+أربعة يا ستة حسب النادقاتش هذا من وين دخل إذا دخل
+
+182
+00:13:18,490 --> 00:13:21,770
+من ملية أسبرتات Shuttle يعني ستة إذا دخل من
+
+183
+00:13:21,770 --> 00:13:26,460
+Glossophosphate Shuttle يعني أربعة اتنينبعدين
+
+184
+00:13:26,460 --> 00:13:29,840
+للمرحلة اللي بعدها تحويل ال pyruvate إلى CoA لأسد
+
+185
+00:13:29,840 --> 00:13:34,440
+ال CoA نتج عنها اتنين ندقاتش هذه حادثات في داخل ال
+
+186
+00:13:34,440 --> 00:13:38,580
+mitochondrial فبضربها في تلاتة ع طول الندقاتش ف2×3
+
+187
+00:13:38,580 --> 00:13:43,230
+بيعطيني 6 ايتمCitric Acid Cycle أو الكريبس سايكل
+
+188
+00:13:43,230 --> 00:13:49,350
+نتج عنها 2 ATP 6 ندقاتش و 2 فدقاتش 2 6 ندقاتش
+
+189
+00:13:49,350 --> 00:13:53,870
+بضروبها ب 3 بتصير 18 ATP و 2 فدقاتش 2 بضروبها ب 2
+
+190
+00:13:53,870 --> 00:13:59,150
+بتعطيني 4 ATP فالمحصلة النهائية لل ATP أنها 4 ATP
+
+191
+00:13:59,150 --> 00:14:06,600
+بشكل مباشر و 32 ل 34 عبارة عنATP على شكل ند قدش
+
+192
+00:14:06,600 --> 00:14:13,460
+وفد قدش يعني كل الـ Total ATP كان من 36 ل 38
+
+193
+00:14:13,460 --> 00:14:16,540
+الإلكترون
+
+194
+00:14:16,540 --> 00:14:20,000
+الترانسبورت تشان انه المية التكندرية في إلها 2
+
+195
+00:14:20,000 --> 00:14:23,340
+ممبران في إلها ممبران داخلي وممبران خارج الإنر
+
+196
+00:14:23,340 --> 00:14:28,180
+والأعطد تيجي أيونات الـ H plus أيونات الهيدروجين
+
+197
+00:14:28,180 --> 00:14:31,320
+تنحشر accumulate between these two membranes
+
+198
+00:14:31,320 --> 00:14:35,820
+تتراكم بين هدول الـ two membranesتبدأ تنتقل في أن
+
+199
+00:14:35,820 --> 00:14:39,020
+سايتو كروم موجودة في هذا الـ membrane تبدأ تنتقل
+
+200
+00:14:39,020 --> 00:14:44,200
+الـ H بلاس الالكترون يتحرك من مولوكيل لمولوكيل حتى
+
+201
+00:14:44,200 --> 00:14:48,580
+يتعامل مع أكسجين و هيدروجين أيون تشكل موتر تبدأ
+
+202
+00:14:48,580 --> 00:14:52,200
+الـ H بلاس تنتقل من سايتو كروم إلى الآخر وصولا
+
+203
+00:14:52,200 --> 00:14:56,500
+للمستقبل النهائي للالكترونات اللي هو الـ oxygenالـ
+
+204
+00:14:56,500 --> 00:15:00,540
+Last Electron Acceptor اللي هو الأكسجين بيتحد الـ
+
+205
+00:15:00,540 --> 00:15:05,360
+H بلس مع الـ O2 بيعطيني H2O فبينتج عن الـ Electron
+
+206
+00:15:05,360 --> 00:15:09,080
+Transport Chain مية H2O
+
+207
+00:15:17,380 --> 00:15:21,960
+مجرد أن ينتقل الاتش بلاست في الـ cytochrome تنتقل
+
+208
+00:15:21,960 --> 00:15:27,560
+مع ناد اتش والفاد اتش وتحويلها إلى طاقة شكل ATP
+
+209
+00:15:27,560 --> 00:15:33,490
+وتخزينها لحاجة الجسمنقطة من 1 ل 4 هدول بدنا نشيها
+
+210
+00:15:33,490 --> 00:15:36,730
+النقطة الخامسة بتحكي ليه ان ال electron transport
+
+211
+00:15:36,730 --> 00:15:40,170
+chain generates no ATP directly ان ال electron
+
+212
+00:15:40,170 --> 00:15:46,340
+transport chain بيمتجش عنها طاقة بشكل مباشرهنا شكل
+
+213
+00:15:46,340 --> 00:15:50,820
+كأنه شكل للـ Electron Transport Chain أنه بتكون
+
+214
+00:15:50,820 --> 00:15:54,460
+عبارة عن دوائل Cytochrome بينتقل فيها الـ H Plus
+
+215
+00:15:54,460 --> 00:15:57,760
+من دائرة للتاريخ من الـ Cytochrome للآخر وصولا
+
+216
+00:15:57,760 --> 00:16:00,960
+لآخر أشياء الحمرة اللي تحت اللي هي الـ Oxygen اللي
+
+217
+00:16:00,960 --> 00:16:03,700
+بنعتبرها الـ last electron acceptor والمستقبل
+
+218
+00:16:03,700 --> 00:16:08,440
+النهائي للإلكترونات تحت معها الـ H وبيعطيني ميه
+
+219
+00:16:08,440 --> 00:16:14,910
+H2Oالـ fermentation اللي هي anaerobic respiration
+
+220
+00:16:14,910 --> 00:16:19,290
+إنه إذا مش موجود أكسجين الجسم بعد خطوة ال
+
+221
+00:16:19,290 --> 00:16:22,570
+glycolysis بدي يعمل fermentation anaerobic
+
+222
+00:16:22,570 --> 00:16:26,370
+respiration في الخميرة بنسميها الكحولك
+
+223
+00:16:26,370 --> 00:16:30,590
+fermentation ليش؟ لأنه حكينا إنه النتج النهائي بعد
+
+224
+00:16:30,590 --> 00:16:35,970
+ال fermentation بينتج الكحول أو إيسانونThe rate of
+
+225
+00:16:35,970 --> 00:16:38,830
+fermentation can be affected by several factors
+
+226
+00:16:38,830 --> 00:16:45,930
+معدل التخمر يتأثر بعدد عوام معدل التخمر يتأثر بعدد
+
+227
+00:16:45,930 --> 00:16:52,050
+عوام اول عامل تركيز الخميرة concentration of yeast
+
+228
+00:16:52,400 --> 00:16:56,000
+تركيز الخميرة بيأثر على معدل ال fermentation لأن
+
+229
+00:16:56,000 --> 00:17:00,040
+الكائن الحي هاد هو المسؤول عن التنفس إذا كانت
+
+230
+00:17:00,040 --> 00:17:04,580
+موجودة و تتنفس كمية أكبر كل ما زادت كمية الخميرة
+
+231
+00:17:04,580 --> 00:17:08,440
+هيزيد معدل التنفس، كل كائن حد يعمل تنفس، فكل ما
+
+232
+00:17:08,440 --> 00:17:12,640
+زادت هيزيد التنفس concentration of glucose تركيز
+
+233
+00:17:12,640 --> 00:17:17,990
+السكة، مش هيبدأ سكر علشان تكسر و تنتج الطاقةفكل ما
+
+234
+00:17:17,990 --> 00:17:22,710
+زاد السكر هيزيد عملية التنفس عشان تنتج الطاقة
+
+235
+00:17:22,710 --> 00:17:28,670
+وكذلك التمرد شرق الحرارة بتأثر عليه for the yeast
+
+236
+00:17:28,670 --> 00:17:31,010
+cell, this chemical reaction is necessary to
+
+237
+00:17:31,010 --> 00:17:34,350
+produce energy for life بالنسبة للخميرة هذه
+
+238
+00:17:34,350 --> 00:17:37,150
+العملية اللي هي fermentation أو الcellularism
+
+239
+00:17:37,150 --> 00:17:41,530
+علشان التنفس هي ضرورية لإنتاج الطاقةالكحول
+
+240
+00:17:41,530 --> 00:17:45,130
+والكربون ديوكسيد مصادره من خلال عملية الدييست
+
+241
+00:17:45,130 --> 00:17:51,670
+بينتج النتج أن العملية بيكون عند كحول وCO2The
+
+242
+00:17:51,670 --> 00:17:54,370
+chemical reaction known as fermentation هي العملية
+
+243
+00:17:54,370 --> 00:17:58,070
+مسميها fermentation كيف بدأ أقيصها Watched and
+
+244
+00:17:58,070 --> 00:18:01,250
+measured by the amount of carbon dioxide gas that
+
+245
+00:18:01,250 --> 00:18:06,050
+is produced من كمية الـCO2 الناتجة بعرف جداش كمية
+
+246
+00:18:06,050 --> 00:18:10,770
+التنفس الخلوي الناتجة من تكسير الجليكوز ال yeast
+
+247
+00:18:10,770 --> 00:18:15,090
+اللي هي السكرومي السرفاشية بتعيش في وجود الأكسجين
+
+248
+00:18:15,090 --> 00:18:19,670
+وفي عدم وجود الأكسجينإذا الـ yeast بتعيش في ظروف
+
+249
+00:18:19,670 --> 00:18:23,750
+هوائية أو لا هوائية زي ما حكينا إنها facultative
+
+250
+00:18:23,750 --> 00:18:28,930
+anaerobic in the presence of oxygen إذا الخميرة
+
+251
+00:18:28,930 --> 00:18:33,560
+كانت متوفرة عندها oxygenماذا ستفعل؟ ستفعل ايروبيك
+
+252
+00:18:33,560 --> 00:18:38,680
+ريسبيراشين هي تبدأ بالجليكوليس ثم بالجليكوليس ثم
+
+253
+00:18:38,680 --> 00:18:40,700
+بالجليكوليس ثم بالجليكوليس ثم بالجليكوليس ثم
+
+254
+00:18:40,700 --> 00:18:42,040
+بالجليكوليس ثم بالجليكوليس ثم بالجليكوليس ثم
+
+255
+00:18:42,040 --> 00:18:42,260
+بالجليكوليس ثم بالجليكوليس ثم بالجليكوليس ثم
+
+256
+00:18:42,260 --> 00:18:43,680
+بالجليكوليس ثم بالجليكوليس ثم بالجليكوليس ثم
+
+257
+00:18:43,680 --> 00:18:48,560
+بالجليكوليس ثم بالجليكوليس ثم بالجليكوليس ثم
+
+258
+00:18:48,560 --> 00:18:52,660
+بالجليكوليس ثم بالجليكوليس ثم بالجليكوليس ثم
+
+259
+00:18:52,660 --> 00:18:57,470
+بالجليكوليس ثم بالجليكوليس ثلو الأكسجين ليس موجود
+
+260
+00:18:57,470 --> 00:19:02,750
+لكن ييست فقط تحصل على طاقة من جليكوليسات وبعدها
+
+261
+00:19:02,750 --> 00:19:06,930
+تحول إلى إيتانول إن هي عملية fermentation إذا لم
+
+262
+00:19:06,930 --> 00:19:10,690
+تكن موجودة ستبدأ جليكوليسات وبعدها سيكون هناك
+
+263
+00:19:10,690 --> 00:19:14,960
+fermentation وانتاج للكحول للإيتانولبحكي له إن هذه
+
+264
+00:19:14,960 --> 00:19:18,160
+less efficient than aerobic respiration هي أقل
+
+265
+00:19:18,160 --> 00:19:22,980
+الكفاءة من ال aerobic respiration ال anaerobic أقل
+
+266
+00:19:22,980 --> 00:19:26,600
+الكفاءة كمية الطاقة اللي بتنتج منها أقل the main
+
+267
+00:19:26,600 --> 00:19:30,620
+source of carbon and energy is glucose مصدر الطاقة
+
+268
+00:19:30,620 --> 00:19:34,160
+الرئيسي منه وحكينا الجليكوز بتم في البداية تكسير
+
+269
+00:19:34,160 --> 00:19:40,550
+الجليكوز تمام؟لو الجلوكوز موجود بتركيز عالي بيصير
+
+270
+00:19:40,550 --> 00:19:46,370
+عندنا إشارة لخلايا أنه تبدأ عملية respiration عشان
+
+271
+00:19:46,370 --> 00:19:50,910
+تكسر الجلوكوز وتنتج الطاقة بيحكي للخميرة ممكن
+
+272
+00:19:50,910 --> 00:19:54,250
+تستخدم سكر تاني غير الجلوكوز مش بس الجلوكوز عندها
+
+273
+00:19:54,250 --> 00:19:58,850
+مصدر طاقة ممكن الساكروز تحوله لأن الساكروز هو سكر
+
+274
+00:19:58,850 --> 00:20:03,090
+ثنائي عبارة عن جلوكوز و fractose في عندها أنزيم
+
+275
+00:20:03,090 --> 00:20:10,180
+اسمه invertaseالانزيم الانفرتاز بيكسر السكروز إلى
+
+276
+00:20:10,180 --> 00:20:15,260
+جلوكوز وفركتوز انزيم الانفرتاز بيكسر السكروز إلى
+
+277
+00:20:15,260 --> 00:20:19,320
+جلوكوز وفركتوز بتقدر تاخد الجلوكوز و تستخدمه في
+
+278
+00:20:19,320 --> 00:20:23,360
+إنتاج الطاقة كمان سكر تاني ممكن تستخدمه الخميرة
+
+279
+00:20:23,360 --> 00:20:28,350
+اللي هو الملتوزإنه عندها إنزيم اسمه منازه بيكسر
+
+280
+00:20:28,350 --> 00:20:31,950
+الملتوز إلى جلوكوز وجلوكوز لإن احنا عارفين إن
+
+281
+00:20:31,950 --> 00:20:36,270
+الملتوز هو سكر ثنائي عبارة عن اتحاد جلوكوز مع
+
+282
+00:20:36,270 --> 00:20:41,050
+جلوكوز فبتكسر الإنزيم المنازه بيكسر الملتوز إلى
+
+283
+00:20:41,050 --> 00:20:46,790
+جلوكوز وجلوكوز بتستخدمهم في إنتاج الطاقةهنا عندنا
+
+284
+00:20:46,790 --> 00:20:51,950
+مخططة ان الخميرة ايش هتمشي بالبداية سواء في اكسجين
+
+285
+00:20:51,950 --> 00:20:55,890
+او فيش اكسجين فوق هتستهل اللي هو فوق هي مكتوب انه
+
+286
+00:20:55,890 --> 00:20:59,490
+هتاخد الجليكوز تعمل عليها عملية ال glycolysis
+
+287
+00:20:59,490 --> 00:21:04,490
+هتعطين اتنين بيروفيت تمام؟ اتنين بيروفيت هدول بعدك
+
+288
+00:21:04,490 --> 00:21:08,710
+اذا الخميرة عندها اكسجين هتروح على جهة اليسار اللي
+
+289
+00:21:08,710 --> 00:21:13,630
+هي anaerobic aerobic respiration اللي هو تنفس هواء
+
+290
+00:21:14,300 --> 00:21:17,800
+اليامين إذا الخميرة ما فيش عندها أكسجين الـ
+
+291
+00:21:17,800 --> 00:21:22,900
+pyruvate هتهيأ يلجأ إلى anaerobic respiration اللي
+
+292
+00:21:22,900 --> 00:21:28,420
+هي إنتاج ال Ethanol و الكحول في ما عملنا اليوم
+
+293
+00:21:28,420 --> 00:21:33,200
+نجيب خميرة و نجيب سكةنحطهم مع بعض و ندوبهم في شوية
+
+294
+00:21:33,200 --> 00:21:38,020
+ماء و نشوف قداش معدل التنفس الخلوي اللي نتج من
+
+295
+00:21:38,020 --> 00:21:42,940
+خلال قياس كمية الـCO2 اللي نتجت فاحنا بدنا نعمل
+
+296
+00:21:42,940 --> 00:21:47,140
+study of cellular respiration in yeast ايش الهدف
+
+297
+00:21:47,140 --> 00:21:51,460
+من ما عملنا study cellular respiration in yeast by
+
+298
+00:21:51,460 --> 00:21:56,600
+measuring the amount of CO2 produced من خلال قياس
+
+299
+00:21:56,600 --> 00:22:01,260
+كمية الـCO2 اللي بتنتجبنستخدم جهاز اسمه
+
+300
+00:22:01,260 --> 00:22:05,600
+Respirometer ال Respirometer هي أداء device to
+
+301
+00:22:05,600 --> 00:22:08,420
+measure the rate of respiration of living organism
+
+302
+00:22:08,420 --> 00:22:11,580
+by measuring the rate of the exchange of oxygen
+
+303
+00:22:11,580 --> 00:22:16,480
+and or carbon dioxide هي أداء بنستخدمها لقياس معدل
+
+304
+00:22:16,480 --> 00:22:21,520
+التنفس الخلوالـ Spirometer أداة هنستخدمها لقياس
+
+305
+00:22:21,520 --> 00:22:26,980
+معدل التنفس الخلوة من خلال قياس كمية ال Oxygen أو
+
+306
+00:22:26,980 --> 00:22:30,980
+الـ CO2 النهجة هذه الأداة هنتعرف عليها و هورجيكوا
+
+307
+00:22:30,980 --> 00:22:35,260
+إياها في المعنى بدنا محلول سكر شجر جليكوز طبيعي
+
+308
+00:22:35,260 --> 00:22:39,840
+بدنا سكر و بدنا yeast الكائن الحي أحطله سكر عشان
+
+309
+00:22:39,840 --> 00:22:44,020
+يستهلك السكر هذا و ينتج الطاقة و بدي مي أدومهم فيه
+
+310
+00:22:45,000 --> 00:22:49,540
+خطوات العمل هذا الجدول ناقص و أنا هصورلكم إياه
+
+311
+00:22:49,540 --> 00:22:54,560
+خطوات العمل إن أنا بدي أجيب خمسة تيوبات تمام؟ بدي
+
+312
+00:22:54,560 --> 00:22:59,300
+أجيب خمسة تيوبات الخمسة تيوبات هدول بدي أقمهم من
+
+313
+00:22:59,300 --> 00:23:03,060
+واحد لخمسة و أشتغل عليهم إيش بدي أعمل؟ تيوب رقم
+
+314
+00:23:03,060 --> 00:23:07,560
+واحد بدي أحط عليها أربعة مل من الميه و سفر من
+
+315
+00:23:07,560 --> 00:23:10,820
+اليست بديش أحط عليها يست خالص و بدي أحط عليها
+
+316
+00:23:10,820 --> 00:23:15,370
+تلاتة مل من السكربتيوب رقم واحد أنا ماحطيتش yeast
+
+317
+00:23:15,370 --> 00:23:18,630
+بس حطيت سكر مع ماء هل هيصير فيها cellular
+
+318
+00:23:18,630 --> 00:23:23,570
+respiration؟ أكيد مش هيصير فيها ومش هينتج عنه CO2
+
+319
+00:23:23,570 --> 00:23:27,930
+ولا أكسجين مش هشوف CO2 ناتج ليش؟ لأنه فيش الكائن
+
+320
+00:23:27,930 --> 00:23:30,970
+الحي اللي بديعمللي عملية التنفس الخلوي ال yeast مش
+
+321
+00:23:30,970 --> 00:23:35,370
+موجودة أنا ماضفتش yeast بتيوب رقم اتنين بدي أحط
+
+322
+00:23:35,370 --> 00:23:40,590
+ستة مل من المياه على واحد مل من ال yeast و صفر مل
+
+323
+00:23:40,590 --> 00:23:45,050
+من السكر بديش أحط سكرطب أنا ماحطيتش سكر، بس حطيت
+
+324
+00:23:45,050 --> 00:23:47,990
+خميرة و معمار، هل هيصير فيها برضه cellular
+
+325
+00:23:47,990 --> 00:23:53,190
+respiration؟ لأ برضه مش هيصير، ليش؟ لأنه الكائن
+
+326
+00:23:53,190 --> 00:23:57,630
+الحي موجود، بس مصدر الطاقة اللي هو السكر مش موجود،
+
+327
+00:23:57,630 --> 00:24:00,530
+أنا ماحطيتش سكر، طب إيش هي بدها تستخدم عشان تعمل
+
+328
+00:24:00,530 --> 00:24:03,630
+.. تنتج الطاقة؟ فمش هيصير فيها برضه cellular
+
+329
+00:24:03,630 --> 00:24:08,710
+respirationطبعا تيوب واحد و اتنين عاملهم ك
+
+330
+00:24:08,710 --> 00:24:13,050
+negative control كcontrol عشان اشوف ان انا اشتغل
+
+331
+00:24:13,050 --> 00:24:16,770
+الصح ولا لا المفروض تيوب واحد و اتنين مايطلعش فيها
+
+332
+00:24:16,770 --> 00:24:20,710
+سي واتوا تيوب رقم تلاتة و اربعة و خمسة هم اللي بده
+
+333
+00:24:20,710 --> 00:24:24,310
+يشتغل عليهم اش بده احط على تيوب رقم تلاتة؟ بده احط
+
+334
+00:24:24,310 --> 00:24:30,170
+عليها تلاتة مل من ميه على واحد مل من الخميرةعلى 3
+
+335
+00:24:30,170 --> 00:24:35,450
+مل سكر، ده حاط 3 مل من الميه مع 1 مل من الخميرة
+
+336
+00:24:35,450 --> 00:24:39,270
+على 3 مل من السكر، بشوف كمية التنفس اللي بتصير
+
+337
+00:24:39,270 --> 00:24:44,410
+عندى، الـCO2 اللي بتطلعطيوب رقم 4 بدي أضع عليها 1
+
+338
+00:24:44,410 --> 00:24:50,450
+مل من المي على 3 مل من الخميرة على 3 مل من السكة
+
+339
+00:24:50,450 --> 00:24:55,910
+وأشوف كمية الـCO2 طيوب رقم 5 بدي أضع عليها 2 مل من
+
+340
+00:24:55,910 --> 00:25:02,910
+المي على 2 مل من الخميرة على 3 مل من السكة يعني
+
+341
+00:25:02,910 --> 00:25:07,230
+أنا في هذه الخطواتفي تيوب رقم 3 أو 4 أو 5 ثبتت
+
+342
+00:25:07,230 --> 00:25:11,830
+كمية السكر في التلت قنابيب حطيت 3 مل من السكر كنت
+
+343
+00:25:11,830 --> 00:25:17,630
+أغير كمية الخميرة حطيت 1 مل على تيوب رقم 3 و 3 مل
+
+344
+00:25:17,630 --> 00:25:22,990
+على تيوب رقم 4 و 2 مل على تيوب رقم 5 فالسكر مش
+
+345
+00:25:22,990 --> 00:25:27,850
+هيأثر مين اللي بيأثر هنا كمية الخميرة كل ما تزيد
+
+346
+00:25:27,850 --> 00:25:32,430
+الكائن الحي الموجود هيزيد معدل التنفس الخلونفأي
+
+347
+00:25:32,430 --> 00:25:36,490
+تيوب هنتوقع أن يكون فيها تنفس خلوي أكتر؟ أكيد
+
+348
+00:25:36,490 --> 00:25:40,450
+التيوب رقم أربع لأنه بتحتوي على yeast أكتر بتحتوي
+
+349
+00:25:40,450 --> 00:25:44,290
+على تلاتة مل أما تيوب تلاتة بتحتوي بس على واحد مل
+
+350
+00:25:44,290 --> 00:25:49,130
+تيوب خمسة بتحتوي على اتنين ملفتيوب رقم 4 هي أكتر
+
+351
+00:25:49,130 --> 00:25:52,810
+الكائن الحي موجود فيها بكمية أكبر ال yeast موجودة
+
+352
+00:25:52,810 --> 00:25:58,390
+أكتر then ال CO2 هتنتج عنها أكبر إشي هنا خطوات
+
+353
+00:25:58,390 --> 00:26:02,170
+العمل بعد ما أعمل في الخمسة تيوبات هدول بجيب جهاز
+
+354
+00:26:02,170 --> 00:26:08,890
+ال Respirometer بحطه في ال هاد في التيوب و بسحب
+
+355
+00:26:08,890 --> 00:26:12,990
+السائل بقى أرفعه في هذا الجهازطبعا هنا مش هتفهموا
+
+356
+00:26:12,990 --> 00:26:15,790
+كيف انه هذا الجهاز لما ناخده في المعمل هورجيكوا
+
+357
+00:26:15,790 --> 00:26:20,330
+الجهاز و كيف هنحط فيه المحلول و بعدين لكي اللي
+
+358
+00:26:20,330 --> 00:26:23,810
+عملنا الجدول اللي قبل و بعدين باخد هذا الجهاز و
+
+359
+00:26:23,810 --> 00:26:28,630
+بحطه في ال spectrophotometer بحطه في ال water bath
+
+360
+00:26:28,630 --> 00:26:32,950
+بحطه على درجة حرارة سابطة عشان مايأثرش و بشوف كمية
+
+361
+00:26:32,950 --> 00:26:36,790
+ال CO2 اللي بتنتج هذا هنشوفه في معمل الجهاز عشان
+
+362
+00:26:36,790 --> 00:26:41,630
+نفهم أكتر و هيك بنكون خلصنا و يعطيكوا العافية
+
diff --git a/PL9fwy3NUQKwZ1Q7zOg1ObgufPMjRI3WY1/h8ODbKj_UcE_raw.json b/PL9fwy3NUQKwZ1Q7zOg1ObgufPMjRI3WY1/h8ODbKj_UcE_raw.json
new file mode 100644
index 0000000000000000000000000000000000000000..3169f6d3bced263f6d440fd8774d595bd9b782da
--- /dev/null
+++ b/PL9fwy3NUQKwZ1Q7zOg1ObgufPMjRI3WY1/h8ODbKj_UcE_raw.json
@@ -0,0 +1 @@
+{"segments": [{"id": 1, "seek": 1396, "start": 0.0, "end": 13.96, "text": "نحكي في آخر معمل عنا اللي هو عبارة عن معمل energy metabolism in yeast تفاعلات إنتاج الطاقة في الكائنات الحية والكائن الحي اللي هنحكي عنه اليوم هو الخميرة", "tokens": [1863, 5016, 4117, 1829, 8978, 19753, 34740, 20449, 42213, 6225, 8315, 13672, 1829, 31439, 6225, 3555, 9640, 3660, 18871, 20449, 42213, 2281, 31190, 294, 21629, 6055, 5172, 995, 30241, 9307, 11933, 29399, 26108, 41950, 995, 28671, 8978, 33251, 16373, 8315, 2655, 21542, 10632, 16070, 4117, 16373, 1863, 21542, 1829, 13672, 1829, 8032, 1863, 5016, 4117, 1829, 18871, 3224, 45595, 20498, 31439, 33962, 2304, 48923], "avg_logprob": -0.17055288461538462, "compression_ratio": 1.5802469135802468, "no_speech_prob": 1.9371509552001953e-05, "words": [{"start": 0.0, "end": 0.72, "word": "نحكي", "probability": 0.765289306640625}, {"start": 0.72, "end": 0.84, "word": " في", "probability": 0.9423828125}, {"start": 0.84, "end": 1.26, "word": " آخر", "probability": 0.8232421875}, {"start": 1.26, "end": 1.66, "word": " معمل", "probability": 0.904052734375}, {"start": 1.66, "end": 2.06, "word": " عنا", "probability": 0.6080322265625}, {"start": 2.06, "end": 2.88, "word": " اللي", "probability": 0.742919921875}, {"start": 2.88, "end": 3.1, "word": " هو", "probability": 0.91943359375}, {"start": 3.1, "end": 3.58, "word": " عبارة", "probability": 0.9591064453125}, {"start": 3.58, "end": 3.8, "word": " عن", "probability": 0.99365234375}, {"start": 3.8, "end": 4.18, "word": " معمل", "probability": 0.887451171875}, {"start": 4.18, "end": 4.7, "word": " energy", "probability": 0.70361328125}, {"start": 4.7, "end": 5.5, "word": " metabolism", "probability": 0.9345703125}, {"start": 5.5, "end": 5.92, "word": " in", "probability": 0.923828125}, {"start": 5.92, "end": 6.28, "word": " yeast", "probability": 0.93896484375}, {"start": 6.28, "end": 7.7, "word": " تفاعلات", "probability": 0.90810546875}, {"start": 7.7, "end": 8.3, "word": " إنتاج", "probability": 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{"id": 2, "seek": 4093, "start": 15.89, "end": 40.93, "text": "أولا نحكي عن الـ Cellular Respiration اللي هو التنفس الخلوي إيش هو التنفس الخلوي؟ هو set of metabolic reactions and processes that take place in the cell of organisms to convert biochemical energy from nutrients into ATP مجموعة من التفاعلات الحيوية في داخل خلايا الكائنات الحية للحصول على الطاقة", "tokens": [10721, 12610, 995, 8717, 5016, 4117, 1829, 18871, 2423, 39184, 28859, 1040, 22480, 7611, 13672, 1829, 31439, 16712, 1863, 36178, 33962, 1211, 45865, 11933, 1829, 8592, 31439, 16712, 1863, 36178, 33962, 1211, 45865, 22807, 31439, 992, 295, 36464, 12215, 293, 7555, 300, 747, 1081, 294, 264, 2815, 295, 22110, 281, 7620, 12198, 34114, 2281, 490, 17617, 666, 39202, 3714, 7435, 2304, 2407, 27884, 9154, 16712, 5172, 995, 30241, 9307, 21542, 1829, 2407, 10632, 8978, 11778, 47283, 1211, 16490, 15040, 25528, 33251, 16373, 8315, 2655, 21542, 10632, 24976, 5016, 9381, 12610, 15844, 41950, 995, 28671], "avg_logprob": 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"probability": 0.980712890625}, {"start": 49.34, "end": 49.54, "word": " في", "probability": 0.85400390625}, {"start": 49.54, "end": 49.96, "word": " داخل", "probability": 0.9680989583333334}, {"start": 49.96, "end": 50.36, "word": " خلايا", "probability": 0.9514973958333334}, {"start": 50.36, "end": 50.96, "word": " الجينات", "probability": 0.930419921875}, {"start": 50.96, "end": 51.48, "word": " الحية", "probability": 0.896728515625}, {"start": 51.48, "end": 52.54, "word": " لإنتاج", "probability": 0.9718017578125}, {"start": 52.54, "end": 53.1, "word": " الطاقة", "probability": 0.9949544270833334}, {"start": 53.1, "end": 53.3, "word": " على", "probability": 0.9423828125}, {"start": 53.3, "end": 53.66, "word": " شكل", "probability": 0.959716796875}, {"start": 53.66, "end": 54.46, "word": " ATP", "probability": 0.9736328125}, {"start": 54.46, "end": 54.98, "word": " من", "probability": 0.9912109375}, {"start": 54.98, "end": 55.6, "word": " الطعام", "probability": 0.9949544270833334}, 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تكسير،", "probability": 0.870654296875}, {"start": 71.22, "end": 71.72, "word": " مركبة", "probability": 0.7771809895833334}, {"start": 71.72, "end": 72.16, "word": " كبيرة", "probability": 0.9889322916666666}, {"start": 72.16, "end": 72.42, "word": " إلى", "probability": 0.95703125}, {"start": 72.42, "end": 73.1, "word": " صغيرة", "probability": 0.9943033854166666}, {"start": 73.1, "end": 74.18, "word": " بينتج", "probability": 0.73828125}, {"start": 74.18, "end": 74.6, "word": " عنها", "probability": 0.992431640625}, {"start": 74.6, "end": 75.06, "word": " طاقة", "probability": 0.99072265625}, {"start": 75.06, "end": 77.34, "word": " نحكي", "probability": 0.9366455078125}, {"start": 77.34, "end": 77.48, "word": " عن", "probability": 0.98876953125}, {"start": 77.48, "end": 77.78, "word": " الكهن", "probability": 0.7420247395833334}, {"start": 77.78, "end": 78.16, "word": " الحي", "probability": 0.7900390625}, {"start": 78.16, "end": 78.26, "word": " اللي", "probability": 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source to grow and thrive هي الكائن حي بيحتاج إلى بيئة دافعة و راطبة و يحتاج إلى مصدر طعام لنمو و المعيشة عشان تنمو و تعيش بدها بيئة راطبة تكون و دافعة و بدها مصدر غذاء هي unicellular أحدية الخلية", "tokens": [6027, 1829, 14851, 39896, 2647, 22110, 300, 3651, 257, 4561, 11, 8641, 2823, 293, 257, 1755, 4009, 281, 1852, 293, 21233, 39896, 33251, 16373, 1863, 11331, 1829, 4724, 1829, 33753, 26108, 30731, 4724, 1829, 19986, 3660, 11778, 31845, 27884, 4032, 12602, 41193, 49401, 4032, 7251, 33753, 26108, 30731, 3714, 9381, 3215, 2288, 23032, 3615, 10943, 5296, 1863, 2304, 2407, 4032, 9673, 3615, 1829, 8592, 3660, 6225, 8592, 7649, 6055, 1863, 2304, 2407, 4032, 37279, 1829, 8592, 47525, 11296, 4724, 1829, 19986, 3660, 12602, 41193, 49401, 6055, 30544, 4032, 11778, 31845, 27884, 4032, 47525, 11296, 3714, 9381, 3215, 2288, 32771, 8848, 16606, 39896, 517, 573, 285, 1040, 5551, 24401, 10632, 33962, 1211, 10632], "avg_logprob": -0.12368639275036027, "compression_ratio": 1.6095617529880477, 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131.66, "end": 131.96, "word": " oval", "probability": 0.96728515625}, {"start": 131.96, "end": 132.72, "word": " بيداوي", "probability": 0.7984619140625}, {"start": 132.72, "end": 133.42, "word": " وإذا", "probability": 0.8250325520833334}, {"start": 133.42, "end": 133.8, "word": " كانت", "probability": 0.9892578125}, {"start": 133.8, "end": 134.38, "word": " بتنقسم", "probability": 0.9715576171875}, {"start": 134.38, "end": 134.84, "word": " بتكون", "probability": 0.935302734375}, {"start": 134.84, "end": 135.34, "word": " مطلعة", "probability": 0.80224609375}, {"start": 135.34, "end": 135.92, "word": " تبرع", "probability": 0.9060872395833334}, {"start": 135.92, "end": 136.54, "word": " بضم", "probability": 0.616455078125}], "temperature": 1.0}, {"id": 7, "seek": 15383, "start": 138.85, "end": 153.83, "text": "cellular respiration بيحكي لإنه في هذا المعمل will be using yeast to measure the rate of cellular respiration لنستخدم الخميرة عشان نقيس معدل التنفس الخلوي عندها كدهش معدل التنفس الخلوي عند الخميرة", "tokens": [4164, 1040, 1597, 7611, 4724, 1829, 5016, 4117, 1829, 5296, 28814, 1863, 3224, 8978, 23758, 9673, 25957, 1211, 486, 312, 1228, 21629, 281, 3481, 264, 3314, 295, 29267, 1597, 7611, 5296, 1863, 14851, 9778, 40448, 33962, 2304, 48923, 6225, 8592, 7649, 8717, 38436, 3794, 20449, 3215, 1211, 16712, 1863, 36178, 33962, 1211, 45865, 43242, 11296, 9122, 3215, 3224, 8592, 20449, 3215, 1211, 16712, 1863, 36178, 33962, 1211, 45865, 43242, 33962, 2304, 48923], "avg_logprob": -0.17380136823000975, "compression_ratio": 1.6514285714285715, "no_speech_prob": 7.748603820800781e-07, "words": [{"start": 138.85, "end": 139.65, "word": "cellular", "probability": 0.6107177734375}, {"start": 139.65, "end": 140.49, "word": " respiration", "probability": 0.88671875}, {"start": 140.49, "end": 141.15, "word": " بيحكي", "probability": 0.838623046875}, {"start": 141.15, "end": 141.43, "word": " لإنه", "probability": 0.651702880859375}, {"start": 141.43, "end": 141.55, "word": " في", "probability": 0.9501953125}, {"start": 141.55, "end": 141.85, "word": " هذا", "probability": 0.89111328125}, {"start": 141.85, "end": 142.57, "word": " المعمل", "probability": 0.9879557291666666}, {"start": 142.57, "end": 142.83, "word": " will", "probability": 0.361328125}, {"start": 142.83, "end": 143.15, "word": " be", "probability": 0.94580078125}, {"start": 143.15, "end": 143.63, "word": " using", "probability": 0.95263671875}, {"start": 143.63, "end": 143.99, "word": " yeast", "probability": 0.87939453125}, {"start": 143.99, "end": 144.17, "word": " to", "probability": 0.9638671875}, {"start": 144.17, "end": 144.45, "word": " measure", "probability": 0.9033203125}, {"start": 144.45, "end": 144.57, "word": " the", "probability": 0.86474609375}, {"start": 144.57, "end": 144.75, "word": " rate", "probability": 0.94921875}, {"start": 144.75, "end": 144.91, "word": " of", "probability": 0.9677734375}, {"start": 144.91, "end": 145.19, "word": " cellular", "probability": 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"probability": 0.99560546875}], "temperature": 1.0}, {"id": 38, "seek": 87344, "start": 844.78, "end": 873.44, "text": "ATP على شكل ند قدش وفد قدش يعني كل الـ Total ATP كان من 36 ل 38 الإلكترون الترانسبورت تشان انه المية التكندرية في إلها 2 ممبران في إلها ممبران داخلي وممبران خارج الإنر والأعطد تيجي أيونات الـ H plus أيونات الهيدروجين تنحشر accumulate between these two membranes تتراكم بين هدول الـ two membranes", "tokens": [2218, 47, 15844, 13412, 28820, 8717, 3215, 12174, 3215, 8592, 4032, 5172, 3215, 12174, 3215, 8592, 37495, 22653, 28242, 2423, 39184, 23170, 39202, 25961, 9154, 8652, 5296, 12843, 33688, 23275, 2655, 2288, 11536, 16712, 2288, 7649, 35457, 13063, 2655, 6055, 8592, 7649, 16472, 3224, 9673, 10632, 16712, 19452, 3215, 2288, 10632, 8978, 11933, 1211, 11296, 568, 3714, 2304, 26890, 7649, 8978, 11933, 1211, 11296, 3714, 2304, 26890, 7649, 11778, 47283, 20292, 4032, 2304, 2304, 26890, 7649, 16490, 9640, 7435, 33688, 1863, 2288, 4032, 6027, 10721, 3615, 9566, 3215, 6055, 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"word": " يعني", "probability": 0.847412109375}, {"start": 847.84, "end": 848.3, "word": " كل", "probability": 0.86474609375}, {"start": 848.3, "end": 848.6, "word": " الـ", "probability": 0.793701171875}, {"start": 848.6, "end": 849.2, "word": " Total", "probability": 0.372314453125}, {"start": 849.2, "end": 849.68, "word": " ATP", "probability": 0.74169921875}, {"start": 849.68, "end": 850.16, "word": " كان", "probability": 0.951171875}, {"start": 850.16, "end": 851.82, "word": " من", "probability": 0.7177734375}, {"start": 851.82, "end": 852.38, "word": " 36", "probability": 0.7568359375}, {"start": 852.38, "end": 852.72, "word": " ل", "probability": 0.658203125}, {"start": 852.72, "end": 853.46, "word": " 38", "probability": 0.60107421875}, {"start": 853.46, "end": 856.54, "word": " الإلكترون", "probability": 0.800927734375}, {"start": 856.54, "end": 857.42, "word": " الترانسبورت", "probability": 0.7635701497395834}, {"start": 857.42, "end": 857.94, "word": " تشان", "probability": 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{"start": 862.42, "end": 862.86, "word": " خارج", "probability": 0.9947916666666666}, {"start": 862.86, "end": 863.34, "word": " الإنر", "probability": 0.4932861328125}, {"start": 863.34, "end": 864.32, "word": " والأعطد", "probability": 0.5725301106770834}, {"start": 864.32, "end": 865.24, "word": " تيجي", "probability": 0.82525634765625}, {"start": 865.24, "end": 865.82, "word": " أيونات", "probability": 0.7803955078125}, {"start": 865.82, "end": 866.0, "word": " الـ", "probability": 0.88330078125}, {"start": 866.0, "end": 866.18, "word": " H", "probability": 0.7802734375}, {"start": 866.18, "end": 866.78, "word": " plus", "probability": 0.08306884765625}, {"start": 866.78, "end": 867.48, "word": " أيونات", "probability": 0.8411865234375}, {"start": 867.48, "end": 868.18, "word": " الهيدروجين", "probability": 0.957763671875}, {"start": 868.18, "end": 869.22, "word": " تنحشر", "probability": 0.9095458984375}, {"start": 869.22, "end": 870.0, "word": " accumulate", "probability": 0.47900390625}, {"start": 870.0, "end": 870.36, "word": " between", "probability": 0.8203125}, {"start": 870.36, "end": 870.68, "word": " these", "probability": 0.802734375}, {"start": 870.68, "end": 870.92, "word": " two", "probability": 0.56982421875}, {"start": 870.92, "end": 871.32, "word": " membranes", "probability": 0.7265625}, {"start": 871.32, "end": 871.8, "word": " تتراكم", "probability": 0.8656005859375}, {"start": 871.8, "end": 872.14, "word": " بين", "probability": 0.98974609375}, {"start": 872.14, "end": 872.6, "word": " هدول", "probability": 0.8551432291666666}, {"start": 872.6, "end": 872.8, "word": " الـ", "probability": 0.730224609375}, {"start": 872.8, "end": 872.98, "word": " two", "probability": 0.72509765625}, {"start": 872.98, "end": 873.44, "word": " membranes", "probability": 0.796630859375}], "temperature": 1.0}, {"id": 39, "seek": 89548, "start": 874.02, "end": 895.48, "text": "تبدأ تنتقل في أن سايتو كروم موجودة في هذا الـ membrane تبدأ تنتقل الـ H بلاس الالكترون يتحرك من مولوكيل لمولوكيل حتى يتعامل مع أكسجين و هيدروجين أيون تشكل موتر تبدأ الـ H بلاس تنتقل من سايتو كروم إلى الآخر وصولا للمستقبل النهائي للالكترونات اللي هو الـ oxygen", "tokens": [2655, 44510, 10721, 6055, 29399, 4587, 1211, 8978, 14739, 8608, 995, 36081, 2407, 9122, 2288, 20498, 3714, 29245, 23328, 3660, 8978, 23758, 2423, 39184, 19651, 6055, 44510, 10721, 6055, 29399, 4587, 1211, 2423, 39184, 389, 4724, 15040, 3794, 2423, 6027, 4117, 2655, 2288, 11536, 7251, 2655, 5016, 31747, 9154, 3714, 12610, 2407, 4117, 26895, 5296, 2304, 12610, 2407, 4117, 26895, 11331, 49975, 7251, 2655, 3615, 10943, 1211, 20449, 5551, 4117, 3794, 7435, 9957, 4032, 8032, 25708, 2288, 29245, 9957, 36632, 11536, 6055, 8592, 28820, 3714, 35473, 2288, 6055, 44510, 10721, 2423, 39184, 389, 4724, 15040, 3794, 6055, 29399, 4587, 1211, 9154, 8608, 995, 36081, 2407, 9122, 2288, 20498, 30731, 6024, 95, 34740, 4032, 9381, 12610, 995, 5296, 19528, 14851, 4587, 36150, 28239, 3224, 16373, 1829, 24976, 6027, 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0.9026692708333334}, {"start": 889.14, "end": 889.66, "word": " تنتقل", "probability": 0.9678955078125}, {"start": 889.66, "end": 889.84, "word": " من", "probability": 0.994140625}, {"start": 889.84, "end": 890.26, "word": " سايتو", "probability": 0.972412109375}, {"start": 890.26, "end": 890.54, "word": " كروم", "probability": 0.9554036458333334}, {"start": 890.54, "end": 890.76, "word": " إلى", "probability": 0.97412109375}, {"start": 890.76, "end": 891.3, "word": " الآخر", "probability": 0.88427734375}, {"start": 891.3, "end": 892.2, "word": " وصولا", "probability": 0.87060546875}, {"start": 892.2, "end": 892.98, "word": " للمستقبل", "probability": 0.89833984375}, {"start": 892.98, "end": 893.62, "word": " النهائي", "probability": 0.946533203125}, {"start": 893.62, "end": 894.58, "word": " للالكترونات", "probability": 0.88818359375}, {"start": 894.58, "end": 894.72, "word": " اللي", "probability": 0.916015625}, {"start": 894.72, "end": 894.86, "word": " هو", "probability": 0.97265625}, {"start": 894.86, "end": 895.0, "word": " الـ", "probability": 0.720947265625}, {"start": 895.0, "end": 895.48, "word": " oxygen", "probability": 0.27978515625}], "temperature": 1.0}, {"id": 40, "seek": 90908, "start": 896.2, "end": 909.08, "text": "الـ Last Electron Acceptor اللي هو الأكسجين بيتحد الـ H بلس مع الـ O2 بيعطيني H2O فبينتج عن الـ Electron Transport Chain مية H2O", "tokens": [6027, 39184, 5264, 12575, 2044, 39957, 284, 13672, 1829, 31439, 16247, 4117, 3794, 7435, 9957, 4724, 36081, 24401, 2423, 39184, 389, 4724, 1211, 3794, 20449, 2423, 39184, 422, 17, 4724, 40228, 9566, 9957, 1829, 389, 17, 46, 6156, 21292, 29399, 7435, 18871, 2423, 39184, 12575, 2044, 22309, 33252, 3714, 10632, 389, 17, 46], "avg_logprob": -0.31018519346360807, "compression_ratio": 1.221476510067114, "no_speech_prob": 1.7881393432617188e-07, "words": [{"start": 896.2, "end": 896.5, "word": "الـ", "probability": 0.27117919921875}, {"start": 896.5, "end": 896.86, "word": " Last", "probability": 0.3896484375}, {"start": 896.86, "end": 897.46, "word": " Electron", "probability": 0.72509765625}, {"start": 897.46, "end": 898.3, "word": " Acceptor", "probability": 0.817138671875}, {"start": 898.3, "end": 898.48, "word": " اللي", "probability": 0.825927734375}, {"start": 898.48, "end": 898.6, "word": " هو", "probability": 0.98876953125}, {"start": 898.6, "end": 899.26, "word": " الأكسجين", "probability": 0.8380859375}, {"start": 899.26, "end": 900.34, "word": " بيتحد", "probability": 0.78759765625}, {"start": 900.34, "end": 900.54, "word": " الـ", "probability": 0.89111328125}, {"start": 900.54, "end": 900.72, "word": " H", "probability": 0.87939453125}, {"start": 900.72, "end": 901.12, "word": " بلس", "probability": 0.47772216796875}, {"start": 901.12, "end": 901.38, "word": " مع", "probability": 0.92138671875}, {"start": 901.38, "end": 901.62, "word": " الـ", "probability": 0.947021484375}, {"start": 901.62, "end": 902.02, "word": " O2", "probability": 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إلى طاقة شكل ATP وتخزينها لحاجة الجسم", "tokens": [2304, 7435, 2288, 3215, 14739, 7251, 29399, 4587, 1211, 2423, 9307, 8592, 4724, 15040, 14851, 8978, 2423, 39184, 3185, 1353, 339, 11505, 6055, 29399, 4587, 1211, 20449, 8717, 18513, 1975, 2655, 8592, 16070, 5172, 18513, 1975, 2655, 8592, 34683, 5016, 2407, 26895, 11296, 30731, 23032, 995, 28671, 13412, 28820, 39202, 34683, 9778, 11622, 9957, 11296, 5296, 5016, 26108, 3660, 25724, 38251], "avg_logprob": -0.3818044311577274, "compression_ratio": 1.3557046979865772, "no_speech_prob": 5.960464477539063e-08, "words": [{"start": 917.38, "end": 918.3, "word": "مجرد", "probability": 0.5420074462890625}, {"start": 918.3, "end": 918.4, "word": " أن", "probability": 0.57958984375}, {"start": 918.4, "end": 918.82, "word": " ينتقل", "probability": 0.724365234375}, {"start": 918.82, "end": 919.2, "word": " الاتش", "probability": 0.5535888671875}, {"start": 919.2, "end": 919.68, "word": " بلاست", "probability": 0.4697265625}, {"start": 919.68, "end": 919.86, "word": " في", "probability": 0.69580078125}, {"start": 919.86, "end": 920.0, "word": " الـ", "probability": 0.5679931640625}, {"start": 920.0, "end": 920.6, "word": " cytochrome", "probability": 0.880615234375}, {"start": 920.6, "end": 921.96, "word": " تنتقل", "probability": 0.732330322265625}, {"start": 921.96, "end": 922.16, "word": " مع", "probability": 0.9501953125}, {"start": 922.16, "end": 922.6, "word": " ناد", "probability": 0.5616455078125}, {"start": 922.6, "end": 922.94, "word": " اتش", "probability": 0.7613118489583334}, {"start": 922.94, "end": 923.44, "word": " والفاد", "probability": 0.7805989583333334}, {"start": 923.44, "end": 923.94, "word": " اتش", "probability": 0.9488932291666666}, {"start": 923.94, "end": 925.28, "word": " وتحويلها", "probability": 0.711328125}, {"start": 925.28, "end": 925.56, "word": " إلى", "probability": 0.85595703125}, {"start": 925.56, "end": 926.82, "word": " طاقة", "probability": 0.81396484375}, {"start": 926.82, "end": 927.18, "word": " شكل", "probability": 0.891357421875}, {"start": 927.18, "end": 927.56, "word": " ATP", "probability": 0.88134765625}, {"start": 927.56, "end": 928.56, "word": " وتخزينها", "probability": 0.91103515625}, {"start": 928.56, "end": 929.3, "word": " لحاجة", "probability": 0.960693359375}, {"start": 929.3, "end": 929.9, "word": " الجسم", "probability": 0.900146484375}], "temperature": 1.0}, {"id": 42, "seek": 94387, "start": 930.87, "end": 943.87, "text": "نقطة من 1 ل 4 هدول بدنا نشيها النقطة الخامسة بتحكي ليه ان ال electron transport chain generates no ATP directly ان ال electron transport chain بيمتجش عنها طاقة بشكل مباشر", "tokens": [1863, 47432, 3660, 9154, 502, 5296, 1017, 8032, 3215, 12610, 47525, 8315, 8717, 8592, 1829, 11296, 28239, 47432, 3660, 33962, 10943, 3794, 3660, 39894, 5016, 4117, 1829, 32239, 3224, 16472, 2423, 6084, 5495, 5021, 23815, 572, 39202, 3838, 16472, 2423, 6084, 5495, 5021, 4724, 32640, 2655, 7435, 8592, 18871, 11296, 23032, 995, 28671, 4724, 8592, 28820, 3714, 3555, 33599, 2288], "avg_logprob": -0.2138832016069381, "compression_ratio": 1.4319526627218935, "no_speech_prob": 1.7881393432617188e-07, "words": [{"start": 930.87, "end": 931.37, "word": "نقطة", "probability": 0.8614908854166666}, {"start": 931.37, "end": 931.49, "word": " من", "probability": 0.908203125}, {"start": 931.49, "end": 931.79, "word": " 1", "probability": 0.50537109375}, {"start": 931.79, "end": 931.99, "word": " ل", "probability": 0.4208984375}, {"start": 931.99, "end": 932.31, "word": " 4", "probability": 0.58740234375}, {"start": 932.31, "end": 932.77, "word": " هدول", "probability": 0.7296549479166666}, {"start": 932.77, "end": 933.09, "word": " بدنا", "probability": 0.830810546875}, {"start": 933.09, "end": 933.49, "word": " نشيها", "probability": 0.833740234375}, {"start": 933.49, "end": 934.41, "word": " النقطة", "probability": 0.9300130208333334}, {"start": 934.41, "end": 934.85, "word": " الخامسة", "probability": 0.872314453125}, {"start": 934.85, "end": 935.31, "word": " بتحكي", "probability": 0.958251953125}, {"start": 935.31, "end": 935.53, "word": " ليه", "probability": 0.773193359375}, {"start": 935.53, "end": 935.61, "word": " ان", "probability": 0.41796875}, {"start": 935.61, "end": 935.73, "word": " ال", "probability": 0.91552734375}, {"start": 935.73, "end": 936.05, "word": " electron", "probability": 0.2073974609375}, {"start": 936.05, "end": 936.73, "word": " transport", "probability": 0.828125}, {"start": 936.73, "end": 937.21, "word": " chain", "probability": 0.87744140625}, {"start": 937.21, "end": 937.79, "word": " generates", "probability": 0.6455078125}, {"start": 937.79, "end": 938.11, "word": " no", "probability": 0.78857421875}, {"start": 938.11, "end": 938.49, "word": " ATP", "probability": 0.83740234375}, {"start": 938.49, "end": 939.03, "word": " directly", "probability": 0.91015625}, {"start": 939.03, "end": 939.77, "word": " ان", "probability": 0.55615234375}, {"start": 939.77, "end": 939.89, "word": " ال", "probability": 0.9716796875}, {"start": 939.89, "end": 940.17, "word": " electron", "probability": 0.873046875}, {"start": 940.17, "end": 940.83, "word": " transport", "probability": 0.94677734375}, {"start": 940.83, "end": 941.19, "word": " chain", "probability": 0.86328125}, {"start": 941.19, "end": 941.77, "word": " بيمتجش", "probability": 0.7798828125}, {"start": 941.77, "end": 942.11, "word": " عنها", "probability": 0.992431640625}, {"start": 942.11, "end": 942.55, "word": " طاقة", "probability": 0.9881184895833334}, {"start": 942.55, "end": 943.13, "word": " بشكل", "probability": 0.9908854166666666}, {"start": 943.13, "end": 943.87, "word": " مباشر", "probability": 0.9864501953125}], "temperature": 1.0}, {"id": 43, "seek": 96926, "start": 945.56, "end": 969.26, "text": "هنا شكل كأنه شكل للـ Electron Transport Chain أنه بتكون عبارة عن دوائل Cytochrome بينتقل فيها الـ H Plus من دائرة للتاريخ من الـ Cytochrome للآخر وصولا لآخر أشياء الحمرة اللي تحت اللي هي الـ Oxygen اللي بنعتبرها الـ last electron acceptor والمستقبل النهائي للإلكترونات تحت معها الـ H وبيعطيني ميه H2O", "tokens": [3224, 8315, 13412, 28820, 9122, 33456, 3224, 13412, 28820, 24976, 39184, 12575, 2044, 22309, 33252, 14739, 3224, 39894, 30544, 6225, 3555, 9640, 3660, 18871, 11778, 2407, 16373, 1211, 10295, 1353, 339, 11505, 49374, 2655, 4587, 1211, 8978, 11296, 2423, 39184, 389, 7721, 9154, 11778, 16373, 25720, 24976, 2655, 9640, 1829, 9778, 9154, 2423, 39184, 10295, 1353, 339, 11505, 24976, 148, 95, 34740, 4032, 9381, 12610, 995, 5296, 148, 95, 34740, 5551, 8592, 1829, 16606, 21542, 2304, 25720, 13672, 1829, 6055, 33753, 13672, 1829, 39896, 2423, 39184, 16489, 8647, 13672, 1829, 44945, 34268, 26890, 11296, 2423, 39184, 1036, 6084, 3241, 284, 4032, 45340, 14851, 4587, 36150, 28239, 3224, 16373, 1829, 24976, 28814, 23275, 2655, 2288, 11536, 9307, 6055, 33753, 20449, 11296, 2423, 39184, 389, 4032, 21292, 3615, 9566, 1829, 22653, 3714, 1829, 3224, 389, 17, 46], "avg_logprob": 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"end": 960.44, "word": " Oxygen", "probability": 0.81298828125}, {"start": 960.44, "end": 960.96, "word": " اللي", "probability": 0.959228515625}, {"start": 960.96, "end": 961.64, "word": " بنعتبرها", "probability": 0.9052734375}, {"start": 961.64, "end": 961.84, "word": " الـ", "probability": 0.6611328125}, {"start": 961.84, "end": 962.02, "word": " last", "probability": 0.31298828125}, {"start": 962.02, "end": 962.5, "word": " electron", "probability": 0.90869140625}, {"start": 962.5, "end": 963.1, "word": " acceptor", "probability": 0.871826171875}, {"start": 963.1, "end": 963.7, "word": " والمستقبل", "probability": 0.787744140625}, {"start": 963.7, "end": 964.3, "word": " النهائي", "probability": 0.83154296875}, {"start": 964.3, "end": 965.6, "word": " للإلكترونات", "probability": 0.8696986607142857}, {"start": 965.6, "end": 966.52, "word": " تحت", "probability": 0.46240234375}, {"start": 966.52, "end": 966.84, "word": " معها", "probability": 0.862548828125}, {"start": 966.84, "end": 967.02, "word": " الـ", "probability": 0.962890625}, {"start": 967.02, "end": 967.18, "word": " H", "probability": 0.79541015625}, {"start": 967.18, "end": 967.94, "word": " وبيعطيني", "probability": 0.85693359375}, {"start": 967.94, "end": 968.44, "word": " ميه", "probability": 0.6309407552083334}, {"start": 968.44, "end": 969.26, "word": " H2O", "probability": 0.888671875}], "temperature": 1.0}, {"id": 44, "seek": 99379, "start": 971.31, "end": 993.79, "text": "الـ fermentation اللي هي anaerobic respiration إنه إذا مش موجود أكسجين الجسم بعد خطوة ال glycolysis بدي يعمل fermentation anaerobic respiration في الخميرة بنسميها الكحولك fermentation ليش؟ لأنه حكينا إنه النتج النهائي بعد ال fermentation بينتج الكحول أو إيسانون", "tokens": [6027, 39184, 43161, 13672, 1829, 39896, 34178, 260, 22234, 1597, 7611, 36145, 3224, 11933, 15730, 37893, 3714, 29245, 23328, 5551, 4117, 3794, 7435, 9957, 25724, 38251, 39182, 16490, 9566, 2407, 3660, 2423, 22633, 1291, 356, 17122, 4724, 16254, 7251, 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yeast", "tokens": [2278, 3314, 295, 43161, 393, 312, 8028, 538, 2940, 6771, 3714, 22488, 1211, 16712, 9778, 29973, 7251, 2655, 10721, 49115, 4724, 22488, 3215, 6225, 2407, 10943, 3714, 22488, 1211, 16712, 9778, 29973, 7251, 2655, 10721, 49115, 39182, 3215, 6225, 2407, 10943, 1975, 12610, 6225, 10943, 1211, 6055, 31747, 1829, 11622, 33962, 2304, 48923, 9856, 295, 21629], "avg_logprob": -0.2354714933194612, "compression_ratio": 1.5374149659863945, "no_speech_prob": 8.463859558105469e-06, "words": [{"start": 994.95, "end": 995.49, "word": "The", "probability": 0.1439208984375}, {"start": 995.49, "end": 995.79, "word": " rate", "probability": 0.93701171875}, {"start": 995.79, "end": 995.97, "word": " of", "probability": 0.9736328125}, {"start": 995.97, "end": 996.53, "word": " fermentation", "probability": 0.90283203125}, {"start": 996.53, "end": 996.83, "word": " can", "probability": 0.94287109375}, {"start": 996.83, "end": 997.03, "word": " be", "probability": 0.94091796875}, {"start": 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1572.27, "end": 1572.43, "word": " مش", "probability": 0.422607421875}, {"start": 1572.43, "end": 1572.99, "word": " هتفهموا", "probability": 0.97822265625}, {"start": 1572.99, "end": 1573.19, "word": " كيف", "probability": 0.974853515625}, {"start": 1573.19, "end": 1573.39, "word": " انه", "probability": 0.4324951171875}, {"start": 1573.39, "end": 1573.57, "word": " هذا", "probability": 0.91259765625}, {"start": 1573.57, "end": 1574.09, "word": " الجهاز", "probability": 0.9775390625}, {"start": 1574.09, "end": 1574.33, "word": " لما", "probability": 0.93505859375}, {"start": 1574.33, "end": 1574.79, "word": " ناخده", "probability": 0.9654541015625}, {"start": 1574.79, "end": 1574.89, "word": " في", "probability": 0.93505859375}, {"start": 1574.89, "end": 1575.29, "word": " المعمل", "probability": 0.98681640625}, {"start": 1575.29, "end": 1575.79, "word": " هورجيكوا", "probability": 0.8729654947916666}, {"start": 1575.79, "end": 1576.37, "word": " الجهاز", "probability": 0.9576822916666666}, {"start": 1576.37, "end": 1576.99, "word": " و", "probability": 0.96630859375}, {"start": 1576.99, "end": 1577.17, "word": " كيف", "probability": 0.7086181640625}, {"start": 1577.17, "end": 1577.55, "word": " هنحط", "probability": 0.9700927734375}, {"start": 1577.55, "end": 1577.79, "word": " فيه", "probability": 0.934326171875}, {"start": 1577.79, "end": 1578.37, "word": " المحلول", "probability": 0.9842529296875}, {"start": 1578.37, "end": 1579.05, "word": " و", "probability": 0.8779296875}, {"start": 1579.05, "end": 1579.65, "word": " بعدين", "probability": 0.948486328125}, {"start": 1579.65, "end": 1580.17, "word": " لكي", "probability": 0.4889322916666667}, {"start": 1580.17, "end": 1580.33, "word": " اللي", "probability": 0.8388671875}, {"start": 1580.33, "end": 1580.77, "word": " عملنا", "probability": 0.9576822916666666}, {"start": 1580.77, "end": 1581.23, "word": " الجدول", "probability": 0.86376953125}, {"start": 1581.23, "end": 1581.39, "word": " اللي", "probability": 0.940673828125}, {"start": 1581.39, "end": 1581.79, "word": " قبل", "probability": 0.897216796875}, {"start": 1581.79, "end": 1582.39, "word": " و", "probability": 0.759765625}, {"start": 1582.39, "end": 1582.63, "word": " بعدين", "probability": 0.95849609375}, {"start": 1582.63, "end": 1583.07, "word": " باخد", "probability": 0.98974609375}, {"start": 1583.07, "end": 1583.31, "word": " هذا", "probability": 0.92822265625}, {"start": 1583.31, "end": 1583.73, "word": " الجهاز", "probability": 0.984375}, {"start": 1583.73, "end": 1583.81, "word": " و", "probability": 0.96728515625}, {"start": 1583.81, "end": 1584.27, "word": " بحطه", "probability": 0.9871826171875}, {"start": 1584.27, "end": 1584.49, "word": " في", "probability": 0.98779296875}, {"start": 1584.49, "end": 1585.51, "word": " ال", "probability": 0.56396484375}, {"start": 1585.51, "end": 1587.07, "word": " spectrophotometer", "probability": 0.629638671875}, {"start": 1587.07, "end": 1587.73, "word": " بحطه", "probability": 0.9434814453125}, {"start": 1587.73, "end": 1587.83, "word": " في", "probability": 0.97705078125}, {"start": 1587.83, "end": 1587.91, "word": " ال", "probability": 0.951171875}, {"start": 1587.91, "end": 1588.13, "word": " water", "probability": 0.94580078125}, {"start": 1588.13, "end": 1588.63, "word": " bath", "probability": 0.86376953125}, {"start": 1588.63, "end": 1589.13, "word": " بحطه", "probability": 0.9442138671875}, {"start": 1589.13, "end": 1589.31, "word": " على", "probability": 0.94775390625}, {"start": 1589.31, "end": 1589.75, "word": " درجة", "probability": 0.9788411458333334}, {"start": 1589.75, "end": 1590.27, "word": " حرارة", "probability": 0.994140625}, {"start": 1590.27, "end": 1590.63, "word": " سابطة", "probability": 0.751708984375}, {"start": 1590.63, "end": 1590.83, "word": " عشان", "probability": 0.97412109375}, {"start": 1590.83, "end": 1591.69, "word": " مايأثرش", "probability": 0.88076171875}, {"start": 1591.69, "end": 1592.21, "word": " و", "probability": 0.92041015625}, {"start": 1592.21, "end": 1592.57, "word": " بشوف", "probability": 0.9938151041666666}, {"start": 1592.57, "end": 1592.95, "word": " كمية", "probability": 0.99365234375}, {"start": 1592.95, "end": 1593.11, "word": " ال", "probability": 0.90576171875}, {"start": 1593.11, "end": 1593.61, "word": " CO2", "probability": 0.7509765625}, {"start": 1593.61, "end": 1593.93, "word": " اللي", "probability": 0.963623046875}, {"start": 1593.93, "end": 1594.57, "word": " بتنتج", "probability": 0.9695638020833334}, {"start": 1594.57, "end": 1595.17, "word": " هذا", "probability": 0.79931640625}, {"start": 1595.17, "end": 1595.61, "word": " هنشوفه", "probability": 0.98603515625}, {"start": 1595.61, "end": 1595.73, "word": " في", "probability": 0.9814453125}, {"start": 1595.73, "end": 1596.09, "word": " معمل", "probability": 0.81103515625}, {"start": 1596.09, "end": 1596.49, "word": " الجهاز", "probability": 0.9886067708333334}, {"start": 1596.49, "end": 1596.79, "word": " عشان", "probability": 0.9864908854166666}, {"start": 1596.79, "end": 1597.29, "word": " نفهم", "probability": 0.9938151041666666}, {"start": 1597.29, "end": 1598.63, "word": " أكتر", "probability": 0.92822265625}, {"start": 1598.63, "end": 1599.09, "word": " و", "probability": 0.56787109375}, {"start": 1599.09, "end": 1599.29, "word": " هيك", "probability": 0.84912109375}, {"start": 1599.29, "end": 1599.61, "word": " بنكون", "probability": 0.5684814453125}, {"start": 1599.61, "end": 1600.31, "word": " خلصنا", "probability": 0.982666015625}, {"start": 1600.31, "end": 1600.61, "word": " و", "probability": 0.59716796875}, {"start": 1600.61, "end": 1601.11, "word": " يعطيكوا", "probability": 0.8744140625}, {"start": 1601.11, "end": 1601.63, "word": " العافية", "probability": 0.9599609375}], "temperature": 1.0}], "language": "ar", "language_probability": 1.0, "duration": 1602.7109375, "duration_after_vad": 1566.0778437499973}
\ No newline at end of file
diff --git a/PL9fwy3NUQKwZ1Q7zOg1ObgufPMjRI3WY1/h8ODbKj_UcE_raw.srt b/PL9fwy3NUQKwZ1Q7zOg1ObgufPMjRI3WY1/h8ODbKj_UcE_raw.srt
new file mode 100644
index 0000000000000000000000000000000000000000..5f1ab5bea49e892b7403ed12ec6e95d7da445fd8
--- /dev/null
+++ b/PL9fwy3NUQKwZ1Q7zOg1ObgufPMjRI3WY1/h8ODbKj_UcE_raw.srt
@@ -0,0 +1,1448 @@
+1
+00:00:00,000 --> 00:00:04,700
+نحكي في آخر معمل عنا اللي هو عبارة عن معمل energy
+
+2
+00:00:04,700 --> 00:00:09,080
+metabolism in yeast تفاعلات إنتاج الطاقة في
+
+3
+00:00:09,080 --> 00:00:12,900
+الكائنات الحية والكائن الحي اللي هنحكي عنه اليوم
+
+4
+00:00:12,900 --> 00:00:18,510
+هو الخميرةأولا نحكي عن الـ Cellular Respiration
+
+5
+00:00:18,510 --> 00:00:22,690
+اللي هو التنفس الخلوي إيش هو التنفس الخلوي؟ هو set
+
+6
+00:00:22,690 --> 00:00:26,170
+of metabolic reactions and processes that take
+
+7
+00:00:26,170 --> 00:00:29,330
+place in the cell of organisms to convert
+
+8
+00:00:29,330 --> 00:00:34,150
+biochemical energy from nutrients into ATP مجموعة
+
+9
+00:00:34,150 --> 00:00:38,550
+من التفاعلات الحيوية في داخل خلايا الكائنات الحية
+
+10
+00:00:38,550 --> 00:00:45,280
+للحصول على الطاقةأو إنتاج الطاقة على شكل ATP من
+
+11
+00:00:45,280 --> 00:00:49,960
+الطعام اللي بنتناوله إذا هي تفاعلات حيوية في داخل
+
+12
+00:00:49,960 --> 00:00:54,980
+خلايا الجينات الحية لإنتاج الطاقة على شكل ATP من
+
+13
+00:00:54,980 --> 00:01:00,400
+الطعام اللي بنتناولهاتفاعلات إنتاج الطاقة تعتبر
+
+14
+00:01:00,400 --> 00:01:05,980
+catabolic reaction، تفاعلات هدم وليس تفاعلات بناء،
+
+15
+00:01:05,980 --> 00:01:09,180
+break large molecule into smaller one releasing
+
+16
+00:01:09,180 --> 00:01:14,180
+energy عملية تكسير، مركبة كبيرة إلى صغيرة بينتج
+
+17
+00:01:14,180 --> 00:01:19,120
+عنها طاقة نحكي عن الكهن الحي اللي بندرسه اليوم
+
+18
+00:01:19,120 --> 00:01:21,880
+اللي هو ال yeast اسمها العلمي Saccharomyces
+
+19
+00:01:21,880 --> 00:01:26,420
+cerevisiaاليست هي living organisms that require a
+
+20
+00:01:26,420 --> 00:01:29,660
+warm, moist environment and a food source to grow
+
+21
+00:01:29,660 --> 00:01:34,100
+and thrive هي الكائن حي بيحتاج إلى بيئة دافعة و
+
+22
+00:01:34,100 --> 00:01:41,100
+راطبة و يحتاج إلى مصدر طعام لنمو و المعيشة عشان
+
+23
+00:01:41,100 --> 00:01:46,140
+تنمو و تعيش بدها بيئة راطبة تكون و دافعة و بدها
+
+24
+00:01:46,140 --> 00:01:52,060
+مصدر غذاء هي unicellular أحدية الخليةsingle cell
+
+25
+00:01:52,060 --> 00:01:56,920
+facultative anaerobic fungi هي خلايا بتعيش في وجود
+
+26
+00:01:56,920 --> 00:02:00,860
+هي كائنات حية تعيش في وجود ال oxygen أو عدم وجود
+
+27
+00:02:00,860 --> 00:02:04,920
+ال oxygen هي اختيارية تعيش في وجود ال oxygen أو
+
+28
+00:02:04,920 --> 00:02:09,880
+عدم وجود ال oxygen تحت ال microscope كيف شكلها
+
+29
+00:02:09,880 --> 00:02:14,380
+بيكون بيكون شكلها oval بيداوي وإذا كانت بتنقسم
+
+30
+00:02:14,380 --> 00:02:21,150
+بتكون مطلعة تبرع بضمcellular respiration بيحكي
+
+31
+00:02:21,150 --> 00:02:24,450
+لإنه في هذا المعمل will be using yeast to measure
+
+32
+00:02:24,450 --> 00:02:27,770
+the rate of cellular respiration لنستخدم الخميرة
+
+33
+00:02:27,770 --> 00:02:32,050
+عشان نقيس معدل التنفس الخلوي عندها كدهش معدل
+
+34
+00:02:32,050 --> 00:02:37,010
+التنفس الخلوي عند الخميرةالغميرة هي single cell هي
+
+35
+00:02:37,010 --> 00:02:41,590
+حكينا انها حدية الخلية ايو كاريوتك organism حقيقة
+
+36
+00:02:41,590 --> 00:02:46,410
+انها تستهلك الكاربوهيدرات السكريات عشان تنتج ال
+
+37
+00:02:46,410 --> 00:02:50,810
+ATP نفس الطريقة زيها زي النبات والحيوان
+
+38
+00:03:00,090 --> 00:03:04,710
+الخميرة بتحول السكر لمية و Carbon Dioxide عشان
+
+39
+00:03:04,710 --> 00:03:09,730
+تحصل على الطاقة إذا السكر بيدخل و بينتج عنه by
+
+40
+00:03:09,730 --> 00:03:12,610
+product من الcellular respiration بينتج عنه مية
+
+41
+00:03:12,610 --> 00:03:19,100
+water H2O و CO2 و Carbon Dioxide و ATP طاقةThe
+
+42
+00:03:19,100 --> 00:03:21,760
+greater the carbon dioxide production, the greater
+
+43
+00:03:21,760 --> 00:03:25,460
+the rate of cellular respiration كل ما كانت كمية
+
+44
+00:03:25,460 --> 00:03:30,260
+الـ CO2 ناتجة أكبر، كمية أكبر، إذا المعدل التنفسي
+
+45
+00:03:30,260 --> 00:03:35,450
+الخلوي اللي صار عندي أكبرإيش مراحل ال cellular
+
+46
+00:03:35,450 --> 00:03:39,590
+respiration stage of cellular respiration أن تلت
+
+47
+00:03:39,590 --> 00:03:43,750
+مراحل أول مرحلة ال glycolysis اللي بتحدث في ال
+
+48
+00:03:43,750 --> 00:03:48,050
+cytosol أول مرحلة glycolysis في ال cytosol تاني
+
+49
+00:03:48,050 --> 00:03:51,430
+مرحلة ال Krebs Cycle اللي تحدث في ال mitochondrial
+
+50
+00:03:51,430 --> 00:03:56,390
+matrix تالت مرحلة electron transport chain تحدث في
+
+51
+00:03:56,390 --> 00:04:01,190
+inner membrane of mitochondria لذا ال glycolysis
+
+52
+00:04:01,190 --> 00:04:06,070
+في ال cytosol Krebs Cycleالـ mitochondrial matrix
+
+53
+00:04:06,070 --> 00:04:11,030
+التجويف الداخلي للميتوكندرية electron transported
+
+54
+00:04:11,030 --> 00:04:17,420
+chain تحدث في ال membrane الداخلي للميتوكندريةأول
+
+55
+00:04:17,420 --> 00:04:21,100
+مرحلة ال glycolysis هي عملية بتحدث في ال cytosol
+
+56
+00:04:21,100 --> 00:04:24,960
+زي ما حكينا بتحدث في وجود أو عدم وجود ال oxygen
+
+57
+00:04:24,960 --> 00:04:28,080
+with or without the presence of oxygen يعني إذا
+
+58
+00:04:28,080 --> 00:04:32,460
+الجسم محتاج لطاقة إذا في oxygen أو فش oxygen أول
+
+59
+00:04:32,460 --> 00:04:35,980
+مرحلة لازم يبدأها هي ال glycolysis إيش هذه
+
+60
+00:04:35,980 --> 00:04:40,140
+العملية؟ تم تحويل one molecule of glucose into two
+
+61
+00:04:40,140 --> 00:04:45,840
+molecules of pyruvateإنه يكسر سكر الجليكوز وينتج
+
+62
+00:04:45,840 --> 00:04:50,280
+عنه اتنين بيروفات هذه العملية بينتج عنها تونة
+
+63
+00:04:50,280 --> 00:04:55,860
+molecule of ATP بينتج عنها كام محصلة اتنين ATP إذا
+
+64
+00:04:55,860 --> 00:04:59,560
+عملية الـGlycolysis تكسير الجليكوز إلى اتنين
+
+65
+00:04:59,560 --> 00:05:04,640
+بيروفات بينتج عنها في الحقيقة بينتج اربعة ATP ولكن
+
+66
+00:05:04,640 --> 00:05:07,980
+هذه العملية بتستهلك منهم اتنين وبيظل عنده اتنين
+
+67
+00:05:08,560 --> 00:05:12,840
+فهما four molecules of ATP are reproduced ولكن ال
+
+68
+00:05:12,840 --> 00:05:17,080
+net number ال number of ATP جدش العدد هو أربع لكن
+
+69
+00:05:17,080 --> 00:05:19,980
+ال net number الحصل النهائي اللي طلعنا منها من
+
+70
+00:05:19,980 --> 00:05:21,960
+الطاقة هي اتنين ATP
+
+71
+00:05:25,180 --> 00:05:28,420
+the fate of pyruvate هلجت بعد هذه العملية بعد
+
+72
+00:05:28,420 --> 00:05:31,940
+الخطوة الأولى اللى هى ال glycolysis اللى حدثت فيه
+
+73
+00:05:31,940 --> 00:05:35,740
+وجود أو عدم وجود ال oxygen ال pyruvate اللى نتج
+
+74
+00:05:35,740 --> 00:05:41,550
+ايش مصيره مصير ال pyruvateالبيروفيت هذا له مصيرين
+
+75
+00:05:41,550 --> 00:05:45,890
+إذا في حالة وجود الأكسجين بيروح على جهة في حالة
+
+76
+00:05:45,890 --> 00:05:49,710
+عدم وجود الأكسجين بيبدأ حاجة تانية إذا الأكسجين
+
+77
+00:05:49,710 --> 00:05:54,990
+موجود هيدخل هذا البيروفيت على الميتوكندرية يتحول
+
+78
+00:05:54,990 --> 00:05:59,330
+إلى أسيت الكو إيه الأسيت الكو إيه هذه بتدخل على
+
+79
+00:05:59,330 --> 00:06:03,190
+كريبس سايكل وبعدين خطوة تالتة اللي هي ال electron
+
+80
+00:06:03,190 --> 00:06:08,180
+transport chainأول عملية جليكوليسية تحدث في وجود
+
+81
+00:06:08,180 --> 00:06:12,440
+أو عند موجود الأكسجين بيمتج عنها بيروفين بيروفيتها
+
+82
+00:06:12,440 --> 00:06:17,680
+إذا في أكسجين هيكمل aerobic respiration تنفس خلوي
+
+83
+00:06:17,680 --> 00:06:22,640
+هو aerobic respiration اللي هي كربس سايكل و بعدين
+
+84
+00:06:22,640 --> 00:06:27,040
+الكترون تنفس بورتتين إذا فاش أكسجين هيبدأ عملية
+
+85
+00:06:27,040 --> 00:06:31,340
+اسمها fermentation أو anaerobic respiration نسميها
+
+86
+00:06:31,340 --> 00:06:36,100
+fermentation أو anaerobic respirationفي حالة
+
+87
+00:06:36,100 --> 00:06:39,440
+الحيوان والبلاند والنبات في الـ Anaerobic
+
+88
+00:06:39,440 --> 00:06:43,140
+respiration ينتج عنها لاكتك أسد الناتج النهائي
+
+89
+00:06:43,140 --> 00:06:47,520
+لاكتك أسد في حالة اليست والبكتيريا الناتج النهائي
+
+90
+00:06:47,520 --> 00:06:51,660
+هو إيتانول هو كحول إيتانول هذا إذا عدم وجود
+
+91
+00:06:51,660 --> 00:06:55,440
+الأكسجين fermentation في الانمال والبلاند بينتج
+
+92
+00:06:55,440 --> 00:06:58,900
+لاكتك أسد في اليست والبكتيريا بينتج إيتانول أو
+
+93
+00:06:58,900 --> 00:07:04,440
+كحولفعشان هيك بنفسر انه ليش لما تبدأ المجهود عند
+
+94
+00:07:04,440 --> 00:07:07,820
+بدء المجهود بتتعب العضلة ليش؟ لأنه مافيش أكسجين
+
+95
+00:07:07,820 --> 00:07:12,100
+بيوصل للعضلة فالعضلة هتبدأ لعملية fermentation لأن
+
+96
+00:07:12,100 --> 00:07:15,420
+مافيش أكسجين همش هتعمل ال aerobic respiration
+
+97
+00:07:15,420 --> 00:07:18,340
+هتعمل anaerobic respiration اللي هي fermentation
+
+98
+00:07:18,340 --> 00:07:22,900
+وهي ال fermentation بينتج عنها lactic acid بيتراكم
+
+99
+00:07:22,900 --> 00:07:27,480
+في العضلات فبتتعب العضلةهنا مكتوب أن الـ fat of
+
+100
+00:07:27,480 --> 00:07:30,380
+pyruvate مصير الـ pyruvate بيحكي اللي بيدخل الـ
+
+101
+00:07:30,380 --> 00:07:34,440
+mitochondria بيتحول إلى أسيت الكو إيه، الأسيت الكو
+
+102
+00:07:34,440 --> 00:07:38,200
+إيه هي اللي بتبدأ الـ Krebs Cycle بس المركب اللي
+
+103
+00:07:38,200 --> 00:07:41,820
+بيبدأ ال Krebs Cycle هو الأسيت الكو إيه هي اللي
+
+104
+00:07:41,820 --> 00:07:45,380
+بتبدأ ال Krebs Cycle مش ال pyruvate نقطة اتنين و
+
+105
+00:07:45,380 --> 00:07:48,990
+تلاتة بدنا شيهاحكيلي وين ال oxygen is present إذا
+
+106
+00:07:48,990 --> 00:07:52,590
+ال oxygen موجود هيبدأ aerobic respiration اللي هي
+
+107
+00:07:52,590 --> 00:07:56,110
+creeps cycle و بعدين electron transport chain لو
+
+108
+00:07:56,110 --> 00:07:58,870
+ال oxygen not present مش موجود هيصير عندي
+
+109
+00:07:58,870 --> 00:08:02,450
+fermentation ل ال pyruvate هيبدأ anaerobic
+
+110
+00:08:02,450 --> 00:08:07,760
+respiration إذا ال oxygen موجودالاسيت الكو ايه
+
+111
+00:08:07,760 --> 00:08:11,360
+هتحول ال بيروفيت لاسيت الكو ايه اسيت الكو ايه هذا
+
+112
+00:08:11,360 --> 00:08:15,200
+بيبدأ اللي هى ال citric acid cycle ال citric acid
+
+113
+00:08:15,200 --> 00:08:19,620
+cycle هى نفسها ال crib cycle اسم تاني اللى بتحدث
+
+114
+00:08:19,620 --> 00:08:24,100
+فى داخل ال mitochondrial matrix بينتج عنها CO2 و
+
+115
+00:08:24,100 --> 00:08:29,340
+NADH تمام الطاقة اللى بتنتج عنها على شكل NADH ال
+
+116
+00:08:29,340 --> 00:08:33,710
+citric acid cycle هى عبارة عن تمان خطواتهي عبارة
+
+117
+00:08:33,710 --> 00:08:38,490
+عن تمان خطوات تحتاج إلى أنزين و co-enzyme إذا
+
+118
+00:08:38,490 --> 00:08:41,610
+حولها إلى شي حاجة بيصير عندنا glycolysis على
+
+119
+00:08:41,610 --> 00:08:45,090
+اليسار من البداية ال glycolysis بينتج عنها بيروفيت
+
+120
+00:08:45,090 --> 00:08:49,110
+بيروفيت هاد بيدخل على الميتوكندرية بتم تحويله إلى
+
+121
+00:08:49,110 --> 00:08:53,270
+أسيت الكوهين عملية تحويله إلى أسيت الكوهين بينتج
+
+122
+00:08:53,270 --> 00:08:59,250
+عنها CO2 و NADH تمام هذا الأسيت الكوهين هو اللي
+
+123
+00:08:59,250 --> 00:09:05,870
+هيبدأ بـ Krebs Cycleففي عندي اتنين بيروفيات لما
+
+124
+00:09:05,870 --> 00:09:10,970
+تحول لاسد الكو اي نتج عنهم اتنين ناد اش في creeps
+
+125
+00:09:10,970 --> 00:09:15,330
+cycle الاسم التاني حكينا citric acid cycle ويلها
+
+126
+00:09:15,330 --> 00:09:21,590
+اسم تالت تي سي اي تراي كربوكسيليك اسد سايكل اذا ال
+
+127
+00:09:21,590 --> 00:09:25,650
+creeps cycle يلها تلت مسميات creeps cycle, citric
+
+128
+00:09:25,650 --> 00:09:29,610
+acid cycle و تراي كربوكسيليك اسد سايكل تي سي اي
+
+129
+00:09:30,710 --> 00:09:34,290
+تحدث فيه ان الـ Mitochondrial Matrix تحتاج إلى
+
+130
+00:09:34,290 --> 00:09:37,690
+وجود الأكسجين، هذه العملية لا تحدث في عدم وجود
+
+131
+00:09:37,690 --> 00:09:42,030
+الأكسجين، وإنما تحتاج إلى أكسجين، I-Robin في كل
+
+132
+00:09:42,030 --> 00:09:49,320
+واحد جليكوز، بتصير هذه الحلقة مرتينCrip Cycle لكل
+
+133
+00:09:49,320 --> 00:09:53,840
+واحد جليكوز بتصير مرتين عن كل مرة بينتج one ATP
+
+134
+00:09:53,840 --> 00:09:58,220
+تلاتة ندقاش و واحد فادقاش كل حلقة كل دورة بينتج
+
+135
+00:09:58,220 --> 00:10:03,200
+one ATP تلاتة ندقاش و واحد فادقاش طيب لكل واحد
+
+136
+00:10:03,200 --> 00:10:07,480
+جليكوز بتصير مرتين يعني كل واحد جليكوز الناتج
+
+137
+00:10:07,480 --> 00:10:15,000
+هيكون اتنين ATP ستة ندقاش و اتنين فادقاشالنتجة هي
+
+138
+00:10:15,000 --> 00:10:20,240
+انها كريبس سايكل من واحد جيليكوز هو اثنين ATP، ستة
+
+139
+00:10:20,240 --> 00:10:27,180
+ناد أتش واثنين فاد أتشهنا مخطط للعملية ككل أول إشي
+
+140
+00:10:27,180 --> 00:10:31,280
+أن الجليكوز فا الجسم محتاج لطاقة بيبدأ بياخد
+
+141
+00:10:31,280 --> 00:10:34,500
+الجليكوز هاد و بيعمله glycolysis اللي بتحدث في ال
+
+142
+00:10:34,500 --> 00:10:38,020
+cytosol خارج ال mitochondria بيصير ال glycolysis
+
+143
+00:10:38,020 --> 00:10:40,960
+بينتج عنه اتنين بيروفات تحويل الجليكوز لاتنين
+
+144
+00:10:40,960 --> 00:10:44,620
+بيروفات حكينا إنه يتحول لاتنين بيروفات على اليمين
+
+145
+00:10:44,620 --> 00:10:49,620
+جداش بينتج عندي اتنين ATP كمحصلة من تحويل الجليكوز
+
+146
+00:10:49,620 --> 00:10:53,040
+إلى اتنين بيروفات بينتج عندي اتنين ATP و اتنين
+
+147
+00:10:53,040 --> 00:10:56,980
+NADHتمام؟ وجدت الاتنين بيروفيت هاد يدخل
+
+148
+00:10:56,980 --> 00:11:01,380
+الميتوكندريا اللي بدي تم تحويله إلى أست الـKa فتحت
+
+149
+00:11:01,380 --> 00:11:06,980
+البيروفيت في عنا سهمين سهم CO2 وسهم 2 ند اتش هدول
+
+150
+00:11:06,980 --> 00:11:10,700
+من وين أجوا؟ من تحويل البيروفيت إلى أست الـKa
+
+151
+00:11:10,700 --> 00:11:15,440
+فبدنا نكتب تحت البيروفيت أست الـKaبعدين ال acid
+
+152
+00:11:15,440 --> 00:11:19,280
+الكوينيهات بدي يدخل على creeps cycle في ال creeps
+
+153
+00:11:19,280 --> 00:11:23,600
+cycle بتصير عندنا عدة خطوات بينتج عنها اربعة CO2
+
+154
+00:11:23,600 --> 00:11:30,880
+اتنين ATP ستة ناد H و اتنين فاد H2 اللي كانت الناد
+
+155
+00:11:30,880 --> 00:11:34,620
+و الفاد بيدخلوا للنتجين اللي كلهم بيدخلوا على
+
+156
+00:11:34,620 --> 00:11:38,360
+electron transport chain على سلسلة نقل الالكترونات
+
+157
+00:11:38,360 --> 00:11:42,520
+اللي بينتج عنها ميواطربتم تحويل الناد أتش والفاد
+
+158
+00:11:42,520 --> 00:11:48,220
+أتش في هذه السلسلة إلى ATP تمام اجيت هنا عند جدول
+
+159
+00:11:48,220 --> 00:11:52,160
+ملخص للطاقة للناد أتش في عملية ال glycolysis حكينا
+
+160
+00:11:52,160 --> 00:11:56,580
+انه بينتج عنها اتنين ATP كمحصلة و اتنين ناد أتش
+
+161
+00:11:56,580 --> 00:12:00,660
+اذا في ال glycolysis بينتج عنده اتنين ATP و اتنين
+
+162
+00:12:00,660 --> 00:12:04,440
+ناد أتش بتعرفوا ان الناد أتش اذا بدي احوله ل ATP
+
+163
+00:12:04,440 --> 00:12:08,720
+بضربه في تلاتة تمامالناد أتش إذا بدى حوله ل ATP
+
+164
+00:12:08,720 --> 00:12:12,360
+بضربه في تلاتة لكن هذه الناد أتش اللي نتجت في ال
+
+165
+00:12:12,360 --> 00:12:16,440
+glycolysis نتجت في ال cytosol لازم تدخل بالأول على
+
+166
+00:12:16,440 --> 00:12:19,360
+الميتوكندرية و بعدين على ال electrode اسبارتشين
+
+167
+00:12:19,360 --> 00:12:23,200
+عشان تتحول ل ATP هذه الناد أتش كيف ده تدخل ل
+
+168
+00:12:23,200 --> 00:12:27,400
+الميتوكندرية؟ في عندها قناتين في ممرين عشان تدخل
+
+169
+00:12:27,400 --> 00:12:32,250
+الميتوكندرية في ممر بنسميه ملات اسبارتات شطلفي ممر
+
+170
+00:12:32,250 --> 00:12:35,590
+اسمه glycerophosphate shuttle إذا إن الناد أتش
+
+171
+00:12:35,590 --> 00:12:38,870
+اللي نتجته ال glycolysis لازم تدخل الميتوكندريال
+
+172
+00:12:38,870 --> 00:12:42,510
+عشان تحوّلها ل ATB كيف ده تدخل؟ من shuttle، من
+
+173
+00:12:42,510 --> 00:12:46,450
+قناة، في عندنا قناتين لدخول الناد أتش، في قناة
+
+174
+00:12:46,450 --> 00:12:51,170
+اسمها مالية أسبارتية شطر، إذا دخل منها الناد أتش،
+
+175
+00:12:51,170 --> 00:12:55,230
+بعتبره عن تلاتة ATB فالاتنين هدول بدرومهم في تلاتة
+
+176
+00:12:55,230 --> 00:12:59,390
+وبيعطيني ستة ATBإذا دخل النادقاتش من القناة اسمها
+
+177
+00:12:59,390 --> 00:13:04,130
+Glossophosphate Shuttle لأ باعتبر الواحد نادقاتش
+
+178
+00:13:04,130 --> 00:13:08,350
+عن اتنين فبضرب باتنين بتكون اتنين ضرب اتنين بيطلع
+
+179
+00:13:08,350 --> 00:13:11,430
+عندي أربعة فليش كتبلي من أربعة لست ان اتنين
+
+180
+00:13:11,430 --> 00:13:14,210
+نادقاتش هاد لما أحولها ل A to B هيطلع عندي يا
+
+181
+00:13:14,210 --> 00:13:18,490
+أربعة يا ستة حسب النادقاتش هذا من وين دخل إذا دخل
+
+182
+00:13:18,490 --> 00:13:21,770
+من ملية أسبرتات Shuttle يعني ستة إذا دخل من
+
+183
+00:13:21,770 --> 00:13:26,460
+Glossophosphate Shuttle يعني أربعة اتنينبعدين
+
+184
+00:13:26,460 --> 00:13:29,840
+للمرحلة اللي بعدها تحويل ال pyruvate إلى CoA لأسد
+
+185
+00:13:29,840 --> 00:13:34,440
+ال CoA نتج عنها اتنين ندقاتش هذه حادثات في داخل ال
+
+186
+00:13:34,440 --> 00:13:38,580
+mitochondrial فبضربها في تلاتة ع طول الندقاتش ف2×3
+
+187
+00:13:38,580 --> 00:13:43,230
+بيعطيني 6 ايتمCitric Acid Cycle أو الكريبس سايكل
+
+188
+00:13:43,230 --> 00:13:49,350
+نتج عنها 2 ATP 6 ندقاتش و 2 فدقاتش 2 6 ندقاتش
+
+189
+00:13:49,350 --> 00:13:53,870
+بضروبها ب 3 بتصير 18 ATP و 2 فدقاتش 2 بضروبها ب 2
+
+190
+00:13:53,870 --> 00:13:59,150
+بتعطيني 4 ATP فالمحصلة النهائية لل ATP أنها 4 ATP
+
+191
+00:13:59,150 --> 00:14:06,600
+بشكل مباشر و 32 ل 34 عبارة عنATP على شكل ند قدش
+
+192
+00:14:06,600 --> 00:14:13,460
+وفد قدش يعني كل الـ Total ATP كان من 36 ل 38
+
+193
+00:14:13,460 --> 00:14:16,540
+الإلكترون
+
+194
+00:14:16,540 --> 00:14:20,000
+الترانسبورت تشان انه المية التكندرية في إلها 2
+
+195
+00:14:20,000 --> 00:14:23,340
+ممبران في إلها ممبران داخلي وممبران خارج الإنر
+
+196
+00:14:23,340 --> 00:14:28,180
+والأعطد تيجي أيونات الـ H plus أيونات الهيدروجين
+
+197
+00:14:28,180 --> 00:14:31,320
+تنحشر accumulate between these two membranes
+
+198
+00:14:31,320 --> 00:14:35,820
+تتراكم بين هدول الـ two membranesتبدأ تنتقل في أن
+
+199
+00:14:35,820 --> 00:14:39,020
+سايتو كروم موجودة في هذا الـ membrane تبدأ تنتقل
+
+200
+00:14:39,020 --> 00:14:44,200
+الـ H بلاس الالكترون يتحرك من مولوكيل لمولوكيل حتى
+
+201
+00:14:44,200 --> 00:14:48,580
+يتعامل مع أكسجين و هيدروجين أيون تشكل موتر تبدأ
+
+202
+00:14:48,580 --> 00:14:52,200
+الـ H بلاس تنتقل من سايتو كروم إلى الآخر وصولا
+
+203
+00:14:52,200 --> 00:14:56,500
+للمستقبل النهائي للالكترونات اللي هو الـ oxygenالـ
+
+204
+00:14:56,500 --> 00:15:00,540
+Last Electron Acceptor اللي هو الأكسجين بيتحد الـ
+
+205
+00:15:00,540 --> 00:15:05,360
+H بلس مع الـ O2 بيعطيني H2O فبينتج عن الـ Electron
+
+206
+00:15:05,360 --> 00:15:09,080
+Transport Chain مية H2O
+
+207
+00:15:17,380 --> 00:15:21,960
+مجرد أن ينتقل الاتش بلاست في الـ cytochrome تنتقل
+
+208
+00:15:21,960 --> 00:15:27,560
+مع ناد اتش والفاد اتش وتحويلها إلى طاقة شكل ATP
+
+209
+00:15:27,560 --> 00:15:33,490
+وتخزينها لحاجة الجسمنقطة من 1 ل 4 هدول بدنا نشيها
+
+210
+00:15:33,490 --> 00:15:36,730
+النقطة الخامسة بتحكي ليه ان ال electron transport
+
+211
+00:15:36,730 --> 00:15:40,170
+chain generates no ATP directly ان ال electron
+
+212
+00:15:40,170 --> 00:15:46,340
+transport chain بيمتجش عنها طاقة بشكل مباشرهنا شكل
+
+213
+00:15:46,340 --> 00:15:50,820
+كأنه شكل للـ Electron Transport Chain أنه بتكون
+
+214
+00:15:50,820 --> 00:15:54,460
+عبارة عن دوائل Cytochrome بينتقل فيها الـ H Plus
+
+215
+00:15:54,460 --> 00:15:57,760
+من دائرة للتاريخ من الـ Cytochrome للآخر وصولا
+
+216
+00:15:57,760 --> 00:16:00,960
+لآخر أشياء الحمرة اللي تحت اللي هي الـ Oxygen اللي
+
+217
+00:16:00,960 --> 00:16:03,700
+بنعتبرها الـ last electron acceptor والمستقبل
+
+218
+00:16:03,700 --> 00:16:08,440
+النهائي للإلكترونات تحت معها الـ H وبيعطيني ميه
+
+219
+00:16:08,440 --> 00:16:14,910
+H2Oالـ fermentation اللي هي anaerobic respiration
+
+220
+00:16:14,910 --> 00:16:19,290
+إنه إذا مش موجود أكسجين الجسم بعد خطوة ال
+
+221
+00:16:19,290 --> 00:16:22,570
+glycolysis بدي يعمل fermentation anaerobic
+
+222
+00:16:22,570 --> 00:16:26,370
+respiration في الخميرة بنسميها الكحولك
+
+223
+00:16:26,370 --> 00:16:30,590
+fermentation ليش؟ لأنه حكينا إنه النتج النهائي بعد
+
+224
+00:16:30,590 --> 00:16:35,970
+ال fermentation بينتج الكحول أو إيسانونThe rate of
+
+225
+00:16:35,970 --> 00:16:38,830
+fermentation can be affected by several factors
+
+226
+00:16:38,830 --> 00:16:45,930
+معدل التخمر يتأثر بعدد عوام معدل التخمر يتأثر بعدد
+
+227
+00:16:45,930 --> 00:16:52,050
+عوام اول عامل تركيز الخميرة concentration of yeast
+
+228
+00:16:52,400 --> 00:16:56,000
+تركيز الخميرة بيأثر على معدل ال fermentation لأن
+
+229
+00:16:56,000 --> 00:17:00,040
+الكائن الحي هاد هو المسؤول عن التنفس إذا كانت
+
+230
+00:17:00,040 --> 00:17:04,580
+موجودة و تتنفس كمية أكبر كل ما زادت كمية الخميرة
+
+231
+00:17:04,580 --> 00:17:08,440
+هيزيد معدل التنفس، كل كائن حد يعمل تنفس، فكل ما
+
+232
+00:17:08,440 --> 00:17:12,640
+زادت هيزيد التنفس concentration of glucose تركيز
+
+233
+00:17:12,640 --> 00:17:17,990
+السكة، مش هيبدأ سكر علشان تكسر و تنتج الطاقةفكل ما
+
+234
+00:17:17,990 --> 00:17:22,710
+زاد السكر هيزيد عملية التنفس عشان تنتج الطاقة
+
+235
+00:17:22,710 --> 00:17:28,670
+وكذلك التمرد شرق الحرارة بتأثر عليه for the yeast
+
+236
+00:17:28,670 --> 00:17:31,010
+cell, this chemical reaction is necessary to
+
+237
+00:17:31,010 --> 00:17:34,350
+produce energy for life بالنسبة للخميرة هذه
+
+238
+00:17:34,350 --> 00:17:37,150
+العملية اللي هي fermentation أو الcellularism
+
+239
+00:17:37,150 --> 00:17:41,530
+علشان التنفس هي ضرورية لإنتاج الطاقةالكحول
+
+240
+00:17:41,530 --> 00:17:45,130
+والكربون ديوكسيد مصادره من خلال عملية الدييست
+
+241
+00:17:45,130 --> 00:17:51,670
+بينتج النتج أن العملية بيكون عند كحول وCO2The
+
+242
+00:17:51,670 --> 00:17:54,370
+chemical reaction known as fermentation هي العملية
+
+243
+00:17:54,370 --> 00:17:58,070
+مسميها fermentation كيف بدأ أقيصها Watched and
+
+244
+00:17:58,070 --> 00:18:01,250
+measured by the amount of carbon dioxide gas that
+
+245
+00:18:01,250 --> 00:18:06,050
+is produced من كمية الـCO2 الناتجة بعرف جداش كمية
+
+246
+00:18:06,050 --> 00:18:10,770
+التنفس الخلوي الناتجة من تكسير الجليكوز ال yeast
+
+247
+00:18:10,770 --> 00:18:15,090
+اللي هي السكرومي السرفاشية بتعيش في وجود الأكسجين
+
+248
+00:18:15,090 --> 00:18:19,670
+وفي عدم وجود الأكسجينإذا الـ yeast بتعيش في ظروف
+
+249
+00:18:19,670 --> 00:18:23,750
+هوائية أو لا هوائية زي ما حكينا إنها facultative
+
+250
+00:18:23,750 --> 00:18:28,930
+anaerobic in the presence of oxygen إذا الخميرة
+
+251
+00:18:28,930 --> 00:18:33,560
+كانت متوفرة عندها oxygenماذا ستفعل؟ ستفعل ايروبيك
+
+252
+00:18:33,560 --> 00:18:38,680
+ريسبيراشين هي تبدأ بالجليكوليس ثم بالجليكوليس ثم
+
+253
+00:18:38,680 --> 00:18:40,700
+بالجليكوليس ثم بالجليكوليس ثم بالجليكوليس ثم
+
+254
+00:18:40,700 --> 00:18:42,040
+بالجليكوليس ثم بالجليكوليس ثم بالجليكوليس ثم
+
+255
+00:18:42,040 --> 00:18:42,260
+بالجليكوليس ثم بالجليكوليس ثم بالجليكوليس ثم
+
+256
+00:18:42,260 --> 00:18:43,680
+بالجليكوليس ثم بالجليكوليس ثم بالجليكوليس ثم
+
+257
+00:18:43,680 --> 00:18:48,560
+بالجليكوليس ثم بالجليكوليس ثم بالجليكوليس ثم
+
+258
+00:18:48,560 --> 00:18:52,660
+بالجليكوليس ثم بالجليكوليس ثم بالجليكوليس ثم
+
+259
+00:18:52,660 --> 00:18:57,470
+بالجليكوليس ثم بالجليكوليس ثلو الأكسجين ليس موجود
+
+260
+00:18:57,470 --> 00:19:02,750
+لكن ييست فقط تحصل على طاقة من جليكوليسات وبعدها
+
+261
+00:19:02,750 --> 00:19:06,930
+تحول إلى إيتانول إن هي عملية fermentation إذا لم
+
+262
+00:19:06,930 --> 00:19:10,690
+تكن موجودة ستبدأ جليكوليسات وبعدها سيكون هناك
+
+263
+00:19:10,690 --> 00:19:14,960
+fermentation وانتاج للكحول للإيتانولبحكي له إن هذه
+
+264
+00:19:14,960 --> 00:19:18,160
+less efficient than aerobic respiration هي أقل
+
+265
+00:19:18,160 --> 00:19:22,980
+الكفاءة من ال aerobic respiration ال anaerobic أقل
+
+266
+00:19:22,980 --> 00:19:26,600
+الكفاءة كمية الطاقة اللي بتنتج منها أقل the main
+
+267
+00:19:26,600 --> 00:19:30,620
+source of carbon and energy is glucose مصدر الطاقة
+
+268
+00:19:30,620 --> 00:19:34,160
+الرئيسي منه وحكينا الجليكوز بتم في البداية تكسير
+
+269
+00:19:34,160 --> 00:19:40,550
+الجليكوز تمام؟لو الجلوكوز موجود بتركيز عالي بيصير
+
+270
+00:19:40,550 --> 00:19:46,370
+عندنا إشارة لخلايا أنه تبدأ عملية respiration عشان
+
+271
+00:19:46,370 --> 00:19:50,910
+تكسر الجلوكوز وتنتج الطاقة بيحكي للخميرة ممكن
+
+272
+00:19:50,910 --> 00:19:54,250
+تستخدم سكر تاني غير الجلوكوز مش بس الجلوكوز عندها
+
+273
+00:19:54,250 --> 00:19:58,850
+مصدر طاقة ممكن الساكروز تحوله لأن الساكروز هو سكر
+
+274
+00:19:58,850 --> 00:20:03,090
+ثنائي عبارة عن جلوكوز و fractose في عندها أنزيم
+
+275
+00:20:03,090 --> 00:20:10,180
+اسمه invertaseالانزيم الانفرتاز بيكسر السكروز إلى
+
+276
+00:20:10,180 --> 00:20:15,260
+جلوكوز وفركتوز انزيم الانفرتاز بيكسر السكروز إلى
+
+277
+00:20:15,260 --> 00:20:19,320
+جلوكوز وفركتوز بتقدر تاخد الجلوكوز و تستخدمه في
+
+278
+00:20:19,320 --> 00:20:23,360
+إنتاج الطاقة كمان سكر تاني ممكن تستخدمه الخميرة
+
+279
+00:20:23,360 --> 00:20:28,350
+اللي هو الملتوزإنه عندها إنزيم اسمه منازه بيكسر
+
+280
+00:20:28,350 --> 00:20:31,950
+الملتوز إلى جلوكوز وجلوكوز لإن احنا عارفين إن
+
+281
+00:20:31,950 --> 00:20:36,270
+الملتوز هو سكر ثنائي عبارة عن اتحاد جلوكوز مع
+
+282
+00:20:36,270 --> 00:20:41,050
+جلوكوز فبتكسر الإنزيم المنازه بيكسر الملتوز إلى
+
+283
+00:20:41,050 --> 00:20:46,790
+جلوكوز وجلوكوز بتستخدمهم في إنتاج الطاقةهنا عندنا
+
+284
+00:20:46,790 --> 00:20:51,950
+مخططة ان الخميرة ايش هتمشي بالبداية سواء في اكسجين
+
+285
+00:20:51,950 --> 00:20:55,890
+او فيش اكسجين فوق هتستهل اللي هو فوق هي مكتوب انه
+
+286
+00:20:55,890 --> 00:20:59,490
+هتاخد الجليكوز تعمل عليها عملية ال glycolysis
+
+287
+00:20:59,490 --> 00:21:04,490
+هتعطين اتنين بيروفيت تمام؟ اتنين بيروفيت هدول بعدك
+
+288
+00:21:04,490 --> 00:21:08,710
+اذا الخميرة عندها اكسجين هتروح على جهة اليسار اللي
+
+289
+00:21:08,710 --> 00:21:13,630
+هي anaerobic aerobic respiration اللي هو تنفس هواء
+
+290
+00:21:14,300 --> 00:21:17,800
+اليامين إذا الخميرة ما فيش عندها أكسجين الـ
+
+291
+00:21:17,800 --> 00:21:22,900
+pyruvate هتهيأ يلجأ إلى anaerobic respiration اللي
+
+292
+00:21:22,900 --> 00:21:28,420
+هي إنتاج ال Ethanol و الكحول في ما عملنا اليوم
+
+293
+00:21:28,420 --> 00:21:33,200
+نجيب خميرة و نجيب سكةنحطهم مع بعض و ندوبهم في شوية
+
+294
+00:21:33,200 --> 00:21:38,020
+ماء و نشوف قداش معدل التنفس الخلوي اللي نتج من
+
+295
+00:21:38,020 --> 00:21:42,940
+خلال قياس كمية الـCO2 اللي نتجت فاحنا بدنا نعمل
+
+296
+00:21:42,940 --> 00:21:47,140
+study of cellular respiration in yeast ايش الهدف
+
+297
+00:21:47,140 --> 00:21:51,460
+من ما عملنا study cellular respiration in yeast by
+
+298
+00:21:51,460 --> 00:21:56,600
+measuring the amount of CO2 produced من خلال قياس
+
+299
+00:21:56,600 --> 00:22:01,260
+كمية الـCO2 اللي بتنتجبنستخدم جهاز اسمه
+
+300
+00:22:01,260 --> 00:22:05,600
+Respirometer ال Respirometer هي أداء device to
+
+301
+00:22:05,600 --> 00:22:08,420
+measure the rate of respiration of living organism
+
+302
+00:22:08,420 --> 00:22:11,580
+by measuring the rate of the exchange of oxygen
+
+303
+00:22:11,580 --> 00:22:16,480
+and or carbon dioxide هي أداء بنستخدمها لقياس معدل
+
+304
+00:22:16,480 --> 00:22:21,520
+التنفس الخلوالـ Spirometer أداة هنستخدمها لقياس
+
+305
+00:22:21,520 --> 00:22:26,980
+معدل التنفس الخلوة من خلال قياس كمية ال Oxygen أو
+
+306
+00:22:26,980 --> 00:22:30,980
+الـ CO2 النهجة هذه الأداة هنتعرف عليها و هورجيكوا
+
+307
+00:22:30,980 --> 00:22:35,260
+إياها في المعنى بدنا محلول سكر شجر جليكوز طبيعي
+
+308
+00:22:35,260 --> 00:22:39,840
+بدنا سكر و بدنا yeast الكائن الحي أحطله سكر عشان
+
+309
+00:22:39,840 --> 00:22:44,020
+يستهلك السكر هذا و ينتج الطاقة و بدي مي أدومهم فيه
+
+310
+00:22:45,000 --> 00:22:49,540
+خطوات العمل هذا الجدول ناقص و أنا هصورلكم إياه
+
+311
+00:22:49,540 --> 00:22:54,560
+خطوات العمل إن أنا بدي أجيب خمسة تيوبات تمام؟ بدي
+
+312
+00:22:54,560 --> 00:22:59,300
+أجيب خمسة تيوبات الخمسة تيوبات هدول بدي أقمهم من
+
+313
+00:22:59,300 --> 00:23:03,060
+واحد لخمسة و أشتغل عليهم إيش بدي أعمل؟ تيوب رقم
+
+314
+00:23:03,060 --> 00:23:07,560
+واحد بدي أحط عليها أربعة مل من الميه و سفر من
+
+315
+00:23:07,560 --> 00:23:10,820
+اليست بديش أحط عليها يست خالص و بدي أحط عليها
+
+316
+00:23:10,820 --> 00:23:15,370
+تلاتة مل من السكربتيوب رقم واحد أنا ماحطيتش yeast
+
+317
+00:23:15,370 --> 00:23:18,630
+بس حطيت سكر مع ماء هل هيصير فيها cellular
+
+318
+00:23:18,630 --> 00:23:23,570
+respiration؟ أكيد مش هيصير فيها ومش هينتج عنه CO2
+
+319
+00:23:23,570 --> 00:23:27,930
+ولا أكسجين مش هشوف CO2 ناتج ليش؟ لأنه فيش الكائن
+
+320
+00:23:27,930 --> 00:23:30,970
+الحي اللي بديعمللي عملية التنفس الخلوي ال yeast مش
+
+321
+00:23:30,970 --> 00:23:35,370
+موجودة أنا ماضفتش yeast بتيوب رقم اتنين بدي أحط
+
+322
+00:23:35,370 --> 00:23:40,590
+ستة مل من المياه على واحد مل من ال yeast و صفر مل
+
+323
+00:23:40,590 --> 00:23:45,050
+من السكر بديش أحط سكرطب أنا ماحطيتش سكر، بس حطيت
+
+324
+00:23:45,050 --> 00:23:47,990
+خميرة و معمار، هل هيصير فيها برضه cellular
+
+325
+00:23:47,990 --> 00:23:53,190
+respiration؟ لأ برضه مش هيصير، ليش؟ لأنه الكائن
+
+326
+00:23:53,190 --> 00:23:57,630
+الحي موجود، بس مصدر الطاقة اللي هو السكر مش موجود،
+
+327
+00:23:57,630 --> 00:24:00,530
+أنا ماحطيتش سكر، طب إيش هي بدها تستخدم عشان تعمل
+
+328
+00:24:00,530 --> 00:24:03,630
+.. تنتج الطاقة؟ فمش هيصير فيها برضه cellular
+
+329
+00:24:03,630 --> 00:24:08,710
+respirationطبعا تيوب واحد و اتنين عاملهم ك
+
+330
+00:24:08,710 --> 00:24:13,050
+negative control كcontrol عشان اشوف ان انا اشتغل
+
+331
+00:24:13,050 --> 00:24:16,770
+الصح ولا لا المفروض تيوب واحد و اتنين مايطلعش فيها
+
+332
+00:24:16,770 --> 00:24:20,710
+سي واتوا تيوب رقم تلاتة و اربعة و خمسة هم اللي بده
+
+333
+00:24:20,710 --> 00:24:24,310
+يشتغل عليهم اش بده احط على تيوب رقم تلاتة؟ بده احط
+
+334
+00:24:24,310 --> 00:24:30,170
+عليها تلاتة مل من ميه على واحد مل من الخميرةعلى 3
+
+335
+00:24:30,170 --> 00:24:35,450
+مل سكر، ده حاط 3 مل من الميه مع 1 مل من الخميرة
+
+336
+00:24:35,450 --> 00:24:39,270
+على 3 مل من السكر، بشوف كمية التنفس اللي بتصير
+
+337
+00:24:39,270 --> 00:24:44,410
+عندى، الـCO2 اللي بتطلعطيوب رقم 4 بدي أضع عليها 1
+
+338
+00:24:44,410 --> 00:24:50,450
+مل من المي على 3 مل من الخميرة على 3 مل من السكة
+
+339
+00:24:50,450 --> 00:24:55,910
+وأشوف كمية الـCO2 طيوب رقم 5 بدي أضع عليها 2 مل من
+
+340
+00:24:55,910 --> 00:25:02,910
+المي على 2 مل من الخميرة على 3 مل من السكة يعني
+
+341
+00:25:02,910 --> 00:25:07,230
+أنا في هذه الخطواتفي تيوب رقم 3 أو 4 أو 5 ثبتت
+
+342
+00:25:07,230 --> 00:25:11,830
+كمية السكر في التلت قنابيب حطيت 3 مل من السكر كنت
+
+343
+00:25:11,830 --> 00:25:17,630
+أغير كمية الخميرة حطيت 1 مل على تيوب رقم 3 و 3 مل
+
+344
+00:25:17,630 --> 00:25:22,990
+على تيوب رقم 4 و 2 مل على تيوب رقم 5 فالسكر مش
+
+345
+00:25:22,990 --> 00:25:27,850
+هيأثر مين اللي بيأثر هنا كمية الخميرة كل ما تزيد
+
+346
+00:25:27,850 --> 00:25:32,430
+الكائن الحي الموجود هيزيد معدل التنفس الخلونفأي
+
+347
+00:25:32,430 --> 00:25:36,490
+تيوب هنتوقع أن يكون فيها تنفس خلوي أكتر؟ أكيد
+
+348
+00:25:36,490 --> 00:25:40,450
+التيوب رقم أربع لأنه بتحتوي على yeast أكتر بتحتوي
+
+349
+00:25:40,450 --> 00:25:44,290
+على تلاتة مل أما تيوب تلاتة بتحتوي بس على واحد مل
+
+350
+00:25:44,290 --> 00:25:49,130
+تيوب خمسة بتحتوي على اتنين ملفتيوب رقم 4 هي أكتر
+
+351
+00:25:49,130 --> 00:25:52,810
+الكائن الحي موجود فيها بكمية أكبر ال yeast موجودة
+
+352
+00:25:52,810 --> 00:25:58,390
+أكتر then ال CO2 هتنتج عنها أكبر إشي هنا خطوات
+
+353
+00:25:58,390 --> 00:26:02,170
+العمل بعد ما أعمل في الخمسة تيوبات هدول بجيب جهاز
+
+354
+00:26:02,170 --> 00:26:08,890
+ال Respirometer بحطه في ال هاد في التيوب و بسحب
+
+355
+00:26:08,890 --> 00:26:12,990
+السائل بقى أرفعه في هذا الجهازطبعا هنا مش هتفهموا
+
+356
+00:26:12,990 --> 00:26:15,790
+كيف انه هذا الجهاز لما ناخده في المعمل هورجيكوا
+
+357
+00:26:15,790 --> 00:26:20,330
+الجهاز و كيف هنحط فيه المحلول و بعدين لكي اللي
+
+358
+00:26:20,330 --> 00:26:23,810
+عملنا الجدول اللي قبل و بعدين باخد هذا الجهاز و
+
+359
+00:26:23,810 --> 00:26:28,630
+بحطه في ال spectrophotometer بحطه في ال water bath
+
+360
+00:26:28,630 --> 00:26:32,950
+بحطه على درجة حرارة سابطة عشان مايأثرش و بشوف كمية
+
+361
+00:26:32,950 --> 00:26:36,790
+ال CO2 اللي بتنتج هذا هنشوفه في معمل الجهاز عشان
+
+362
+00:26:36,790 --> 00:26:41,630
+نفهم أكتر و هيك بنكون خلصنا و يعطيكوا العافية
+
diff --git a/PL9fwy3NUQKwZ1Q7zOg1ObgufPMjRI3WY1/hVJVg6dDeBU.srt b/PL9fwy3NUQKwZ1Q7zOg1ObgufPMjRI3WY1/hVJVg6dDeBU.srt
new file mode 100644
index 0000000000000000000000000000000000000000..f32e6781a5133691c999999f44e6c38e7d795d4e
--- /dev/null
+++ b/PL9fwy3NUQKwZ1Q7zOg1ObgufPMjRI3WY1/hVJVg6dDeBU.srt
@@ -0,0 +1,734 @@
+1
+00:00:00,000 --> 00:00:03,080
+هنا على الصورة هذه، على الخط اللي فوق أول شيء، هنا
+
+2
+00:00:03,080 --> 00:00:05,680
+عرفنا الصيغة العامة والـ structure العامة للحمض 
+
+3
+00:00:05,680 --> 00:00:09,900
+الأميني إنه بيتكون من جمعة كاربو المركزية مرتبطة
+
+4
+00:00:09,900 --> 00:00:15,820
+بهيدروجين وارتشين وكاربوكسيل وامينوجروب
+
+5
+00:00:15,820 --> 00:00:21,540
+هالخط اللي فوقها على رموز جيت عنه أن أول خط أحمر
+
+6
+00:00:21,540 --> 00:00:25,100
+فوق اللي هي الـ repeat units في عندنا صيغة عاملة
+
+7
+00:00:25,100 --> 00:00:29,320
+الحمض الأميني في في النص مجموعة carbon من فوق مرتبط
+
+8
+00:00:29,320 --> 00:00:33,620
+فيها مجموعة هيدروجين من الأسفل مرتبط فيها الـ R
+
+9
+00:00:33,620 --> 00:00:37,340
+group اللي هي الـ side chain على اليمين مرتبط فيها
+
+10
+00:00:37,340 --> 00:00:41,320
+الـ C double bond O اللي هي الكربوكسيل group على
+
+11
+00:00:41,320 --> 00:00:46,250
+اليسار مرتبط فيها N-H اللي هي مجموعة الأمين
+
+12
+00:00:46,250 --> 00:00:51,070
+الأمينوجروب تمام؟ هي أول حمض أميني جنبه مرتبط فيه
+
+13
+00:00:51,070 --> 00:00:55,550
+حمض أميني تاني اللي هي الكربونة المركزية وهيدروجين
+
+14
+00:00:55,550 --> 00:00:59,730
+في الأعلى و R في الأسفل وعلى اليمين كاربوكسيل جروب
+
+15
+00:00:59,730 --> 00:01:04,810
+وعلى الجمال أمينوجروب هدول مجموعة أحماض أمينية أجت
+
+16
+00:01:04,810 --> 00:01:08,730
+اترتبت جانب بعض مرتبطة مع بعضها البعض في
+
+17
+00:01:08,730 --> 00:01:14,740
+بيبتايدبوند رابطة بيبتيدية فكونتلي البروتين طب وين
+
+18
+00:01:14,740 --> 00:01:19,040
+الـ peptide bond جاية؟ الـ peptide bond أجت ورتبطت
+
+19
+00:01:19,040 --> 00:01:24,780
+بمجموعة كربوكسيل من الحمض الأميني الأول ومجموعة
+
+20
+00:01:24,780 --> 00:01:28,680
+أمين من الحمض الأميني الثاني إذا ملاحظين الحمض
+
+21
+00:01:28,680 --> 00:01:32,300
+الأميني الأول و الحمض الأميني الثاني في بينهم 
+
+22
+00:01:32,300 --> 00:01:35,300
+رابطة هي الرابطة اللي في النص اللي فصلت الحمض
+
+23
+00:01:35,300 --> 00:01:39,680
+الأميني الأول عن الثاني بنسميها peptide bond و
+
+24
+00:01:39,680 --> 00:01:45,880
+موقعها تيجي بين مجموعة كربوكسيل من حمض أميني
+
+25
+00:01:45,880 --> 00:01:51,230
+ومجموعة أمين من حمض أميني آخر ما ش عملة هاي
+
+26
+00:01:51,230 --> 00:01:55,850
+الـpeptide bond هي المفروض ان الـCOOH الكربوكسيلي
+
+27
+00:01:55,850 --> 00:02:01,490
+جروب و الـN و الـAmino group NH2 هي بتعمل على نزع
+
+28
+00:02:01,490 --> 00:02:09,530
+جزء ما تنزع H2O تنزع جزء ما بتاخد من الكربوكسيلي
+
+29
+00:02:09,530 --> 00:02:14,290
+جروب بتاخد منها OH تبدلها C double bond O و بتاخد
+
+30
+00:02:14,290 --> 00:02:19,410
+من الـNH2 بتاخد مجموعة هيدروجين فهيك الصراطين
+
+31
+00:02:19,410 --> 00:02:23,990
+ياخدوا OH من هنا و Hydrogen من هنا يعني H2O اللي
+
+32
+00:02:23,990 --> 00:02:28,450
+هو جزء ماء فالرابطة البيبتيدية تكون الرابطة
+
+33
+00:02:28,450 --> 00:02:34,410
+البيبتيدية بينتج عنها خروج ماء وهذا التفاعل يسمى
+
+34
+00:02:34,410 --> 00:02:39,870
+Condensation Reaction تفاعلات اللي بتنتج عنها خروج
+
+35
+00:02:39,870 --> 00:02:45,970
+ماء بنسميها Condensation Reaction لأنه كون عنها
+
+36
+00:02:45,970 --> 00:02:52,090
+جزء ماء إذا انتفاع التكون الرابط البيبتيدية يعتبر
+
+37
+00:02:52,090 --> 00:02:59,980
+Condensation Reaction لأنه نتج عنه جزيع ماء H2O طيب
+
+38
+00:02:59,980 --> 00:03:03,820
+تمام، إذا البروتين بيتكون عن طريق مجموعة أحماض
+
+39
+00:03:03,820 --> 00:03:08,840
+أمينية ترتبط في روابط بيبتيدية وين بتيجي الرابطة
+
+40
+00:03:08,840 --> 00:03:14,260
+بين مجموعة كربوكسيل ومجموعة أمين؟ إيش بينتج عنها
+
+41
+00:03:14,260 --> 00:03:19,440
+خروج ماء، نزع جزء ماء؟ لذلك سمينا التفاعل تكون
+
+42
+00:03:19,440 --> 00:03:25,990
+رابط البيبتيدية بـ Condensation Reaction اللي جيت
+
+43
+00:03:25,990 --> 00:03:29,370
+على الأطراف في البروتين في البروتين هذا اللي اتكون
+
+44
+00:03:29,370 --> 00:03:32,070
+في السلسلة اللي فوق اللي أنتو شايفينها على
+
+45
+00:03:32,070 --> 00:03:35,910
+الأطراف في حمض أميني فيه مجموعة أمين و group
+
+46
+00:03:35,910 --> 00:03:40,650
+مرتبطتش بحمض أميني آخر وعلى الطرف من الجهة الثانية
+
+47
+00:03:40,650 --> 00:03:44,190
+من اليمين في عندنا مجموعة حمض أميني فيه مجموعة
+
+48
+00:03:44,190 --> 00:03:48,290
+Carboxyl مرتبطتش يعني في عندنا مجموعة Carboxyl
+
+49
+00:03:48,290 --> 00:03:53,330
+free حرة في مجموعة أمين free برضه حرة على الأطراف
+
+50
+00:03:53,330 --> 00:03:57,000
+بتكون هذه بنسميها مجموعة الأمين الحرة بنسميها
+
+51
+00:03:57,000 --> 00:04:02,500
+N-terminus N-terminus هو الحمض الأميني الذي
+
+52
+00:04:02,500 --> 00:04:09,020
+يحتوي على free amino group لم تشارك في تكوين
+
+53
+00:04:09,020 --> 00:04:14,360
+الرابطة البيبتيدية إذا مجموعة الأمين الـ free بتكون
+
+54
+00:04:14,360 --> 00:04:18,700
+الحرة التي لم تشارك في تكوين الرابطة بنسميها
+
+55
+00:04:18,700 --> 00:04:24,380
+N-terminus طب و الـ C-terminus .. C-terminus برضه
+
+56
+00:04:24,380 --> 00:04:29,540
+هي الحمض الأميني اللي يحتوي على free carboxyl
+
+57
+00:04:29,540 --> 00:04:35,580
+group لم تشارك في تكوين الرابط اللي بيبتديها يعني
+
+58
+00:04:35,580 --> 00:04:39,320
+بنفهم احنا عند تكول الـ protein تيجي مجموعة أحماض
+
+59
+00:04:39,320 --> 00:04:44,720
+أمينية ترتبط مع بعضها البعض في روابط بيبتيدية وين
+
+60
+00:04:44,720 --> 00:04:48,440
+بتتكون الروابط البيبتيدية؟ بين Carboxyl group من
+
+61
+00:04:48,440 --> 00:04:53,510
+حمض أميني أميني group من حمض أميني آخر  وشو بتعمل
+
+62
+00:04:53,510 --> 00:04:57,730
+على نزع جزاق ماء؟ بسمّينا التفاعل Condensation
+
+63
+00:04:57,730 --> 00:05:03,110
+Reaction لأنه بينتج عنه جزاق ماء في مجموعة الأحمض
+
+64
+00:05:03,110 --> 00:05:07,350
+الأمينية هاي بيضل على الأطراف مجموعة أمينو جروب مش
+
+65
+00:05:07,350 --> 00:05:12,070
+مرتبطة على الطرف من جهة مجموعة كربوكسيل جروب مش
+
+66
+00:05:12,070 --> 00:05:16,930
+مرتبطة على جهة المجموعة الكربوكسيلة الحرة التي لم
+
+67
+00:05:16,930 --> 00:05:20,450
+ترتبط ولم تشارك في تكوين الرابطة اللي بيبتديها
+
+68
+00:05:20,450 --> 00:05:25,450
+بسميها C-terminus ومجموعة الـ amino group الحرة ال
+
+69
+00:05:25,450 --> 00:05:30,030
+free التي لم تشارك في تكوين الرابطة بسميها N
+
+70
+00:05:30,030 --> 00:05:30,950
+-terminus
+
+71
+00:05:33,570 --> 00:05:37,230
+و أنا classification of amino acid according to source
+
+72
+00:05:37,230 --> 00:05:41,910
+تقسيم الأحماض الأمينية بناء على مصدر بيقسموها
+
+73
+00:05:41,910 --> 00:05:46,330
+لقسمين في عندنا أحماض أمينية أساسية essential
+
+74
+00:05:46,330 --> 00:05:51,470
+amino acid وفي non-essential amino acid غير ضرورية
+
+75
+00:05:51,470 --> 00:05:55,690
+الأحماض الأمينية الأساسية هي الأحماض اللي بيكون
+
+76
+00:05:55,690 --> 00:06:01,130
+الجسم غير قادر على تصنيعها لازم تكون موجودة في
+
+77
+00:06:01,130 --> 00:06:06,020
+الوجبة incapable of forming requests and must be
+
+78
+00:06:06,020 --> 00:06:11,840
+required in time الجسم غير قادر على تصنيعها و لازم
+
+79
+00:06:11,840 --> 00:06:15,340
+تكون موجودة في الواجبات، في الأكل اليومي اللي
+
+80
+00:06:15,340 --> 00:06:21,390
+بيتناوله، فعددها تسعة الـ Non-essential أمينواسد
+
+81
+00:06:21,390 --> 00:06:26,090
+الغير ضرورية الجسم قادر على تصنيحها بيقدر يصنحها
+
+82
+00:06:26,090 --> 00:06:32,130
+مش ضروري أني أتأكد أنها موجودة في وجباتي وعددها 11
+
+83
+00:06:32,130 --> 00:06:36,570
+Non-essential الجسم قادر على تصنيحها Essential
+
+84
+00:06:36,570 --> 00:06:40,610
+الجسم غير قادر على تصنيحها لازم أتأكد أن أنا
+
+85
+00:06:40,610 --> 00:06:46,140
+أخدتها عن طريق الوجبات اليومية وعددها 9 الـ get non
+
+86
+00:06:46,140 --> 00:06:51,240
+-essential أكثر 11 يعني صح ولا خطأ إذا حكيت الجسم
+
+87
+00:06:51,240 --> 00:06:57,480
+قادر على تصنيع معظم الأحماض الأمنية صح الجسم قادر
+
+88
+00:06:57,480 --> 00:07:05,310
+على تصنيع معظم الأحماض الأمنية اللي هي عددها 11 طيب
+
+89
+00:07:05,310 --> 00:07:10,110
+عندنا أبسط حمض أميني هو simplest and smallest
+
+90
+00:07:10,110 --> 00:07:14,050
+amino acid هو عبارة عن الـ glycine الـ glycine هو
+
+91
+00:07:14,050 --> 00:07:19,010
+أبسط حمض أميني ليش؟ لأنه احنا حكينا كل حمض أميني
+
+92
+00:07:19,010 --> 00:07:22,950
+يختلف عن الثاني بالـ side chain بالـ R والـ R في الـ
+
+93
+00:07:22,950 --> 00:07:27,710
+glycine هي عبارة عن ذرة هيدروجين فقط عبارة عن H
+
+94
+00:07:27,710 --> 00:07:33,210
+ذرة هيدروجين فهو بسيط جدا يكون عبارة عن كربونة
+
+95
+00:07:33,210 --> 00:07:33,950
+مركزية
+
+96
+00:07:39,760 --> 00:07:43,140
+ذرة هيدروجين من الجهة الأخرى الـ R-group هي عبارة
+
+97
+00:07:43,140 --> 00:07:47,900
+عن ذرة هيدروجين فهو أبسط حمض أميني لـ glycine أما
+
+98
+00:07:47,900 --> 00:07:52,600
+الحمض الأمين الشهد الفريد هو الـ proline حكينا
+
+99
+00:07:52,600 --> 00:07:57,640
+الـ proline هو الحمض الأمين الوحيد الذي يحتوي على
+
+100
+00:07:57,640 --> 00:08:03,070
+amino group وليس amino group ما يحتويش على مجموعة
+
+101
+00:08:03,070 --> 00:08:06,490
+بنسميها amino group بإنما بنسميها amino group أو
+
+102
+00:08:06,490 --> 00:08:10,630
+secondary amino group بنسميها، ليش؟ لأنه بتكون فيه
+
+103
+00:08:10,630 --> 00:08:15,050
+مجموعة الأمين مرتبطة في الكربون المركزية ومرتبطة
+
+104
+00:08:15,050 --> 00:08:18,730
+مع الـR، مع الـside chain، مع مجموعة الـside chain
+
+105
+00:08:18,730 --> 00:08:23,030
+اللي بتكون موجودة، فهي بنسميها amino group مش
+
+106
+00:08:23,030 --> 00:08:24,610
+amino group
+
+107
+00:08:27,190 --> 00:08:32,330
+بنجي لتصنيف الأحماض الأمنية بناء على الـ polarity
+
+108
+00:08:32,330 --> 00:08:36,290
+كيف بدي أحدد الـ polarity القطبية للأحماض الأمنية؟
+
+109
+00:08:36,290 --> 00:08:39,310
+من خلال النظر إلى الـ sided chain إلى الـ R group
+
+110
+00:08:39,310 --> 00:08:44,010
+هذه مجموعة الـ R إيش أجي مكانها؟ أول شيء تصنيف
+
+111
+00:08:44,010 --> 00:08:47,530
+الأحماض الأمينية بناء على الـ polarity تُقسم إلى
+
+112
+00:08:47,530 --> 00:08:53,250
+قسمين في منها polar قطبية في منها non polar non
+
+113
+00:08:53,250 --> 00:08:57,790
+polar يعني غير قطبية يعني هي الأحماض الأمينية
+
+114
+00:08:57,790 --> 00:09:06,520
+تحتوي على C و H في الـ side chain فقط النانبولان هي
+
+115
+00:09:06,520 --> 00:09:10,420
+الـ amino acid that contains C و H in their side
+
+116
+00:09:10,420 --> 00:09:14,100
+chain لما أجى أطلع على الاري جروب هاي إيش فيها
+
+117
+00:09:14,100 --> 00:09:19,520
+بلاقي فيها بس يا ذرات كربون يا هيدروجين أو كربون
+
+118
+00:09:19,520 --> 00:09:24,470
+وهيدروجين الألاجي في الـ side-chain كربون وهيدروجين
+
+119
+00:09:24,470 --> 00:09:29,050
+فقط، لا تحتوي أي مجموعة قطبية أخرى، وهي غير قادرة
+
+120
+00:09:29,050 --> 00:09:34,250
+على تكوين روابط أيونية ولا هيدروجينية ولا قادرة
+
+121
+00:09:34,250 --> 00:09:39,430
+على كسب أو فقد الإلكترونات، هي الـ non-polar amino
+
+122
+00:09:39,430 --> 00:09:42,930
+acid تكون تحتوي في الـ sided chain على كاربون
+
+123
+00:09:42,930 --> 00:09:47,830
+وهيدروجين فقط غير قادر على تكوين روابط هيدروجينية
+
+124
+00:09:47,830 --> 00:09:54,170
+ولا أيونية ولا قادر على فقد وكسب الإلكترونات الجزء
+
+125
+00:09:54,170 --> 00:09:57,730
+الثاني من الأحماض الأمينية هي الـ polar القطبية
+
+126
+00:09:57,730 --> 00:10:03,810
+القطبية مقسمة لقسمين في منها بتكون قطبية مشحونة
+
+127
+00:10:03,810 --> 00:10:08,050
+وفي قطبية غير مشحونة يعني في منها polar charged
+
+128
+00:10:08,050 --> 00:10:13,530
+وفي polar uncharged الـ Polar سواء كانت charged أو
+
+129
+00:10:13,530 --> 00:10:17,850
+uncharged الـ side chain بيكون يحتوي على مجموعة
+
+130
+00:10:17,850 --> 00:10:22,030
+قطبية لو جيتي على الـ R على الـ side chain تطلع
+
+131
+00:10:22,030 --> 00:10:26,850
+عليه بتلاقي يحتوي على مجموعة قطبية إيش هي
+
+132
+00:10:26,850 --> 00:10:30,930
+المجموعات القطبية؟ ممكن تكون OH اللي هي Hydroxyl
+
+133
+00:10:30,930 --> 00:10:36,290
+group هي مجموعة قطبية OH Hydroxyl group معناها C
+
+134
+00:10:36,290 --> 00:10:42,090
+double bond O اللي هي الكربونيل الـ group CWO هي
+
+135
+00:10:42,090 --> 00:10:46,190
+الكربونيل جروب فهي عندنا الـ sulfhydryl جروب
+
+136
+00:10:46,190 --> 00:10:51,130
+الـ sulfhydryl جروب اللي هي الـSH في عندنا
+
+137
+00:10:51,130 --> 00:10:56,450
+الأمين جروب اللي هي الـH2 أمين جروب هدولة كلهم
+
+138
+00:10:56,450 --> 00:11:00,650
+مجموعات قطبية لو شوفتهم في الـ sided chain بعرف إنه
+
+139
+00:11:00,650 --> 00:11:06,150
+هذا بولا طب هل هو charged ولا uncharged? uncharged
+
+140
+00:11:06,150 --> 00:11:09,110
+يعني ماعلهوش شحنة لا عليه positive ولا عليه
+
+141
+00:11:09,110 --> 00:11:13,170
+negative فيه مجموعة قطبية لكن لا هي positive ولا
+
+142
+00:11:13,170 --> 00:11:17,370
+هي negative طب ليه charged؟ charged معناه إنه
+
+143
+00:11:17,370 --> 00:11:21,470
+يحتوي على مجموعة قطبية بشحنة إما بتكون الشحنة
+
+144
+00:11:21,470 --> 00:11:26,200
+positive لـ charged إذا كانت positive يعني باسأو
+
+145
+00:11:26,200 --> 00:11:32,520
+negative charge يعني أسال إذا الأحماض الأمنية تقسم
+
+146
+00:11:32,520 --> 00:11:37,400
+حسب الـ polarity إلى polar أو non-polar، non-polar
+
+147
+00:11:37,400 --> 00:11:45,790
+لا يحتوي على مجموعة قطبية وإنما يحتوي على بس كربون
+
+148
+00:11:45,790 --> 00:11:49,450
+وهيدروجين في الـ sided chain طب الـ polar يحتوي على
+
+149
+00:11:49,450 --> 00:11:55,290
+مجموعة قطبية إما حكينا هيدروكسيل أو كربونيل أو
+
+150
+00:11:55,290 --> 00:12:00,430
+sulfhydride أو أمين جروب هدول المجموعات القطبية
+
+151
+00:12:00,430 --> 00:12:06,150
+إما تكون uncharged أو charged عليها شحنة أو معلهاش
+
+152
+00:12:06,150 --> 00:12:06,910
+شحنة
+
+153
+00:12:10,230 --> 00:12:13,090
+لو جينا نتفرج على الصور الموجودة هنا أو على الـ
+
+154
+00:12:13,090 --> 00:12:17,190
+structure الأحماض الأمنية الموجودة هنا هذه الصور
+
+155
+00:12:17,190 --> 00:12:21,270
+مقسمة لأحماض الأمنية لـ polar و non-polar في منها
+
+156
+00:12:21,270 --> 00:12:25,310
+charged و في منها uncharged، تمام؟ هلجيت؟ لو جينا
+
+157
+00:12:25,310 --> 00:12:29,890
+على الصورة من فوق لليسار، في عندنا مجموعة، هلجيت؟
+
+158
+00:12:29,890 --> 00:12:33,190
+أنا كيف بتعرف polar ولا non-polar، حاجة هنا؟ نتطلع
+
+159
+00:12:33,190 --> 00:12:35,750
+على الـside chain، على القعر اللي بتكون في الأسفل،
+
+160
+00:12:35,750 --> 00:12:40,190
+هي الملونة لجيت أول حمض أمين لو جينا تطلعنا بنلاقي
+
+161
+00:12:40,190 --> 00:12:43,630
+الـR هي عبارة عن أتش عن هيدروجين حكينا اللي بيكون
+
+162
+00:12:43,630 --> 00:12:47,810
+عنده الـR الـR group عبارة عن هيدروجين أو كربونا
+
+163
+00:12:47,810 --> 00:12:52,400
+أو الاثنين مع بعض بيكون non-polar فهذه المجموعة
+
+164
+00:12:52,400 --> 00:12:57,780
+كلها الجزء اللي فار من اليسار هي non-polar amino
+
+165
+00:12:57,780 --> 00:13:02,980
+acid لأن الـR group تحتوي على كربون وهيدروجين فقط،
+
+166
+00:13:02,980 --> 00:13:08,240
+تمام؟ الجتلة تحت منها على اليسار للأسفل لو طلعنا
+
+167
+00:13:08,240 --> 00:13:14,040
+على الـR group اللي تحت بنلاقيها تحتوي على مجموعة
+
+168
+00:13:14,040 --> 00:13:20,350
+قطبية تحتوي على مجموعة قطبية في منها بتحتوي على
+
+169
+00:13:20,350 --> 00:13:24,670
+هيدروكسيل جروب في منها بتحتوي على أمين جروب في
+
+170
+00:13:24,670 --> 00:13:29,770
+منها بتحتوي على صلفهيدرال جروب S-H المهم كلهم في
+
+171
+00:13:29,770 --> 00:13:34,870
+الـR group يحتوي على واحدة من المجموعات القطبية
+
+172
+00:13:35,420 --> 00:13:39,800
+يعني الأول يحتوي OH، الثاني OH، الثالث الستين
+
+173
+00:13:39,800 --> 00:13:43,440
+يحتوي على SH، self-hydraulic group يعني فيه إنه
+
+174
+00:13:43,440 --> 00:13:47,940
+مجموعات قطبية، يبقى هو polar طب هل عليه شحنة؟ لأ
+
+175
+00:13:47,940 --> 00:13:51,580
+فيش شحنات ولا موجب ولا سالب، إذا هو polar
+
+176
+00:13:51,580 --> 00:13:57,400
+uncharged R group Polar Uncharged Amino Acid لو
+
+177
+00:13:57,400 -->
+
+201
+00:15:41,370 --> 00:15:45,770
+مع الـside chain عشان هيك سميناها amino group أو 
+
+202
+00:15:45,770 --> 00:15:50,830
+secondary amino group مش primary amino group فهو 
+
+203
+00:15:50,830 --> 00:15:52,870
+الحمض الأميني الشاد 
+
+204
+00:15:58,770 --> 00:16:03,530
+لما نشتغل العمل إن شاء الله راح نسير نكتب تقرير
+
+205
+00:16:03,530 --> 00:16:07,230
+نكتب report تقرير عن المعمل اللي احنا اشتغلناه ده
+
+206
+00:16:07,230 --> 00:16:12,330
+نكتب فيه اسم الفحص، الهدف منه، المبدأ العام، خطوات 
+
+207
+00:16:12,330 --> 00:16:17,690
+إجراء التجربة، نكتب النتيجة ونكتب تعليق عليها كمًّا 
diff --git a/PL9fwy3NUQKwZ1Q7zOg1ObgufPMjRI3WY1/hVJVg6dDeBU_postprocess.srt b/PL9fwy3NUQKwZ1Q7zOg1ObgufPMjRI3WY1/hVJVg6dDeBU_postprocess.srt
new file mode 100644
index 0000000000000000000000000000000000000000..8c6082c5a1cf35077eb0ba45f883534b3bb1263a
--- /dev/null
+++ b/PL9fwy3NUQKwZ1Q7zOg1ObgufPMjRI3WY1/hVJVg6dDeBU_postprocess.srt
@@ -0,0 +1,828 @@
+1
+00:00:00,000 --> 00:00:03,080
+هنا على الصورة هذه، على الخط اللي فوق أول شيء، هنا
+
+2
+00:00:03,080 --> 00:00:05,680
+عرفنا الصيغة العامة والـ structure العامة للحمض
+
+3
+00:00:05,680 --> 00:00:09,900
+الأميني إنه بيتكون من جمعة كاربو المركزية مرتبطة
+
+4
+00:00:09,900 --> 00:00:15,820
+بهيدروجين وارتشين وكاربوكسين وامينوبرونلو جينا
+
+5
+00:00:15,820 --> 00:00:21,540
+هالخط اللي فوقها على رموز جيت عنه أن أول خط أحمر
+
+6
+00:00:21,540 --> 00:00:25,100
+فوق اللي هي ال repeat units في عندنا صيغة عاملة
+
+7
+00:00:25,100 --> 00:00:29,320
+الحمد للأمين في في النص مجموعة carbon من فوق مرتبط
+
+8
+00:00:29,320 --> 00:00:33,620
+فيها مجموعة هيدروجين من الأسفل مرتبط فيها ال R
+
+9
+00:00:33,620 --> 00:00:37,340
+group اللي هي ال side chain على اليمين مرتبط فيها
+
+10
+00:00:37,340 --> 00:00:41,320
+ال C double bond O اللي هي الكربوكسيل group على
+
+11
+00:00:41,320 --> 00:00:46,250
+اليسارمرتبط فيها N-H اللي هي مجموعة الأمين
+
+12
+00:00:46,250 --> 00:00:51,070
+الأمينوجروب تمام؟ هي أول حمض أميني جنبه مرتبط فيه
+
+13
+00:00:51,070 --> 00:00:55,550
+حمض أميني تاني اللي هي الكربونة المركزية وهيدروجين
+
+14
+00:00:55,550 --> 00:00:59,730
+في الأعلى وR في الأسفل وعلى اليمين كاربوكسيل جروب
+
+15
+00:00:59,730 --> 00:01:04,810
+وعلى الجمال أمينوجروب هدول مجموعة أحماض أمينية أجت
+
+16
+00:01:04,810 --> 00:01:08,730
+اترتبت جانب بعض مرتبطة مع بعضها البعض في
+
+17
+00:01:08,730 --> 00:01:14,740
+بيبتايدبونرابطة بيبتيديا فكونتلي البروتين طب وين
+
+18
+00:01:14,740 --> 00:01:19,040
+ال peptide bond جاية؟ ال peptide bond أجت ورتبطت
+
+19
+00:01:19,040 --> 00:01:24,780
+بيهمجموعة كربوكسيل من الحمض الأميني الأول ومجموعة
+
+20
+00:01:24,780 --> 00:01:28,680
+أمين من الحمض الأميني التاني إذا ملاحظين الحمض
+
+21
+00:01:28,680 --> 00:01:32,300
+الأميني الأول و الحمض الأميني التاني في بينهم
+
+22
+00:01:32,300 --> 00:01:35,300
+رابطة هي الرابطة اللي في النص اللي فصلت الحمض
+
+23
+00:01:35,300 --> 00:01:39,680
+الأميني الأول عن التاني بنسميها peptide bond و
+
+24
+00:01:39,680 --> 00:01:45,880
+موقعها تيجي بين مجموعة كربوكسيل من حمض أميني
+
+25
+00:01:45,880 --> 00:01:51,230
+ومجموعة أمين من حمض أميني آخرماش عملة هاي
+
+26
+00:01:51,230 --> 00:01:55,850
+الـpeptide bond هي المفروض ان الـCOOH الكربوكسيلي
+
+27
+00:01:55,850 --> 00:02:01,490
+جروب و الـN و الـAmino group NH2 هي بتعمل على نزع
+
+28
+00:02:01,490 --> 00:02:09,530
+جزء ما تنزع H2O تنزع جزء ما بتاخد من الكربوكسيلي
+
+29
+00:02:09,530 --> 00:02:14,290
+جروب بتاخد منها OH تبدلها C double bond O و بتاخد
+
+30
+00:02:14,290 --> 00:02:19,410
+من الـNH2 بتاخد مجموعة هيدروجينفهيك الصراطين
+
+31
+00:02:19,410 --> 00:02:23,990
+ياخدوا OH من هنا و Hydrogen من هنا يعني H2O اللي
+
+32
+00:02:23,990 --> 00:02:28,450
+هو جزء ماء فالرابطة البيبتيدية تكون الرابطة
+
+33
+00:02:28,450 --> 00:02:34,410
+البيبتيدية بينتج عنها خروج ماء وهذا التفاعل يسمى
+
+34
+00:02:34,410 --> 00:02:39,870
+Condensation Reaction تفاعلات اللي بتنتج عنها خروج
+
+35
+00:02:39,870 --> 00:02:45,970
+ماء بنسميها Condensation Reaction لأنه كون عنها
+
+36
+00:02:45,970 --> 00:02:52,090
+جزء ماءإذا انتفاع التكون الرابط البيبتيدية يعتبر
+
+37
+00:02:52,090 --> 00:02:59,980
+Condensation Reaction لأنه نتج عنه جزيع ماء H2Oطيب
+
+38
+00:02:59,980 --> 00:03:03,820
+تمام، إذا البروتين بيتكون عن طريق مجموعة أحماض
+
+39
+00:03:03,820 --> 00:03:08,840
+أمينية ترتبط في روابط بيبتيدية وين بتيجي الرابطة
+
+40
+00:03:08,840 --> 00:03:14,260
+بين مجموعة كربوكسيل ومجموعة أمين؟ إيش بينتج عنها
+
+41
+00:03:14,260 --> 00:03:19,440
+خروج ماء، نزع جزء ماء؟ لذلك سمينا التفاعل تكون
+
+42
+00:03:19,440 --> 00:03:25,990
+رابط البيبتيدية بـ Condensation Reactionاللي جيت
+
+43
+00:03:25,990 --> 00:03:29,370
+على الأطراف في البروتين في البروتين هذا اللي اتكون
+
+44
+00:03:29,370 --> 00:03:32,070
+في السلسلة اللي فوق اللي انتوا شايفينها على
+
+45
+00:03:32,070 --> 00:03:35,910
+الأطراف في حمض أميني فيه مجموعة أمين و group
+
+46
+00:03:35,910 --> 00:03:40,650
+مرتبطتش بحمض أميني آخر وعلى الطرف من الجهة التانية
+
+47
+00:03:40,650 --> 00:03:44,190
+من اليمين في عندنا مجموعة حمض أميني فيه مجموعة
+
+48
+00:03:44,190 --> 00:03:48,290
+Carboxyl مرتبطتش يعني في عندنا مجموعة Carboxyl
+
+49
+00:03:48,290 --> 00:03:53,330
+free حرة في مجموعة أمين free برضه حرة على الأطراف
+
+50
+00:03:53,330 --> 00:03:57,000
+بتكونهذه بنسميها مجموعة الأمين الحرة بنسميها
+
+51
+00:03:57,000 --> 00:04:02,500
+Anterminous Anterminous هو الحمض الأميني الذي
+
+52
+00:04:02,500 --> 00:04:09,020
+يحتوي على free amino group لم تشارك في تكوين
+
+53
+00:04:09,020 --> 00:04:14,360
+الرابطة البيبتدية إذا مجموعة الأمين الـ free بتكون
+
+54
+00:04:14,360 --> 00:04:18,700
+الحرة التي لم تشارك في تكوين الرابطة بنسميها
+
+55
+00:04:18,700 --> 00:04:24,380
+Anterminousطب و الـ C-terminus .. C-terminus برضه
+
+56
+00:04:24,380 --> 00:04:29,540
+هي الحمض الأميني اللي يحتوي على free carboxyl
+
+57
+00:04:29,540 --> 00:04:35,580
+group لم تشارك في تكوين الرابط اللي بيبتديهايعني
+
+58
+00:04:35,580 --> 00:04:39,320
+بنفهم احنا عند تكول ال protein تيجي مجموعة أحماض
+
+59
+00:04:39,320 --> 00:04:44,720
+أمينية ترتبط مع بعضها البعض في روابط بيبتيدية وين
+
+60
+00:04:44,720 --> 00:04:48,440
+بتتكون الروابط البيبتيدية؟ بين Carboxyl group من
+
+61
+00:04:48,440 --> 00:04:53,510
+حمض أميني أميني group من حمض أميني آخروشو بتعمل
+
+62
+00:04:53,510 --> 00:04:57,730
+على نزع جزاق ماء؟ بسمّينا التفاعل Condensation
+
+63
+00:04:57,730 --> 00:05:03,110
+Reaction لأنه بينتج عنه جزاق ماء في مجموعة الأحمض
+
+64
+00:05:03,110 --> 00:05:07,350
+الأميني هاي بيضل على الأطراف مجموعة أمينو جروب مش
+
+65
+00:05:07,350 --> 00:05:12,070
+مرتبطة على الطرف من جهة مجموعة كربوكسيل جروب مش
+
+66
+00:05:12,070 --> 00:05:16,930
+مرتبطة على جهةالمجموعة الكربوكسيلة الحرة اللتي لم
+
+67
+00:05:16,930 --> 00:05:20,450
+ترتبط ولم تشارك في تكوين الرابطة اللي بيبتديها
+
+68
+00:05:20,450 --> 00:05:25,450
+بسميها C-terminus ومجموعة ال amino group الحرة ال
+
+69
+00:05:25,450 --> 00:05:30,030
+free اللتي لم تشارك في تكوين الرابطة بسميها N
+
+70
+00:05:30,030 --> 00:05:30,950
+-terminus
+
+71
+00:05:33,570 --> 00:05:37,230
+وانا classification of amino acid according source
+
+72
+00:05:37,230 --> 00:05:41,910
+تقسيم الأحماض الأمينية بناء على مصدر بيقسموها
+
+73
+00:05:41,910 --> 00:05:46,330
+لقسمين في عندنا أحماض أمينية أساسية essential
+
+74
+00:05:46,330 --> 00:05:51,470
+amino acid وفي non-essential amino acid غير ضرورية
+
+75
+00:05:51,470 --> 00:05:55,690
+أحماض الأمينية الأساسية هي الأحماض اللي بيكون
+
+76
+00:05:55,690 --> 00:06:01,130
+الجسم غير قادر على تصنيعها لازم تكون موجودة في
+
+77
+00:06:01,130 --> 00:06:06,020
+الوجبةincapable of forming requests and must be
+
+78
+00:06:06,020 --> 00:06:11,840
+required in time الجسم غير قادر على تصنيعها و لازم
+
+79
+00:06:11,840 --> 00:06:15,340
+تكون موجودة في الواجبات، في الأكل اليومي اللي
+
+80
+00:06:15,340 --> 00:06:21,390
+بيتناوله، فعددها تسعةالـ Non-essential أمينواسد
+
+81
+00:06:21,390 --> 00:06:26,090
+الغير ضرورية الجسم قادر على تصنيحها بيقدر يصنحها
+
+82
+00:06:26,090 --> 00:06:32,130
+مش ضروري أني أتأكد أنها موجودة في وجباتي وعددها 11
+
+83
+00:06:32,130 --> 00:06:36,570
+Non-essential الجسم قادر على تصنيحها Essential
+
+84
+00:06:36,570 --> 00:06:40,610
+الجسم غير قادر على تصنيحها لازم أتأكد أن أنا
+
+85
+00:06:40,610 --> 00:06:46,140
+أخدتها عن طريق الوجبات اليومية وعددها 9الـ get non
+
+86
+00:06:46,140 --> 00:06:51,240
+-essential أكتر 11 يعني صح ولا خطأ إذا حكيت الجسم
+
+87
+00:06:51,240 --> 00:06:57,480
+قادر على تصنيع معظم الأحماض الأمنية صح الجسم قادر
+
+88
+00:06:57,480 --> 00:07:05,310
+على تصنيع معظم الأحماض الأمنية اللي هي عددها 11طيب
+
+89
+00:07:05,310 --> 00:07:10,110
+عندنا أبسط حمض أميني هو simplest and smallest
+
+90
+00:07:10,110 --> 00:07:14,050
+amino acid هو عبارة عن ال glycine ال glycine هو
+
+91
+00:07:14,050 --> 00:07:19,010
+أبسط حمض أميني ليش؟ لأنه احنا حكينا كل حمض أميني
+
+92
+00:07:19,010 --> 00:07:22,950
+يختلف عن التاني بال side chain بال R وال R في ال
+
+93
+00:07:22,950 --> 00:07:27,710
+glycine هي عبارة عن ذرة هيدروجين فقط عبارة عن H
+
+94
+00:07:27,710 --> 00:07:33,210
+ذرة هيدروجين فهو بسيط جدا يكون عبارة عن كربونة
+
+95
+00:07:33,210 --> 00:07:33,950
+مركزية
+
+96
+00:07:39,760 --> 00:07:43,140
+زرّة هيدروجين من الجهة الأخرى الـ R-group هي عبارة
+
+97
+00:07:43,140 --> 00:07:47,900
+عن زرّة هيدروجين فهو أبسط حمض أميني لـ glycine أما
+
+98
+00:07:47,900 --> 00:07:52,600
+الحمض الأمين الشهد الفريد هو البرولين حكينا
+
+99
+00:07:52,600 --> 00:07:57,640
+البرولين هو الحمض الأمين الوحيد الذي يحتوي على
+
+100
+00:07:57,640 --> 00:08:03,070
+amino group وليس amino groupماحتويش على مجموعة
+
+101
+00:08:03,070 --> 00:08:06,490
+بنسميها amino group بإنما بنسميها amino group أو
+
+102
+00:08:06,490 --> 00:08:10,630
+secondary amino group بنسميها، ليش؟ لأنه بتكون فيه
+
+103
+00:08:10,630 --> 00:08:15,050
+مجموعة الأمين مرتبطة في الكربون المركزية ومرتبطة
+
+104
+00:08:15,050 --> 00:08:18,730
+مع الـR، مع الـside chain، مع مجموعة الـside chain
+
+105
+00:08:18,730 --> 00:08:23,030
+اللي بتكون موجودة، فهي بنسميها amino group مش
+
+106
+00:08:23,030 --> 00:08:24,610
+amino group
+
+107
+00:08:27,190 --> 00:08:32,330
+بنجي لتصنيف الأحماض الأمنية بناء على ال polarity
+
+108
+00:08:32,330 --> 00:08:36,290
+كيف بدي أحدد ال polarity القطبية للأحماض الأمنية؟
+
+109
+00:08:36,290 --> 00:08:39,310
+من خلال النظر إلى ال sided chain إلى ال R group
+
+110
+00:08:39,310 --> 00:08:44,010
+هذه مجموعة ال R إيش أجي مكانها؟أول شيء تصنيف
+
+111
+00:08:44,010 --> 00:08:47,530
+الأحماض الأمينية بناء على الـ polarity تُقسم إلى
+
+112
+00:08:47,530 --> 00:08:53,250
+قسمين في منها polar قطبية في منها non polar non
+
+113
+00:08:53,250 --> 00:08:57,790
+polar يعني غير قطبية يعني هي الأحماض الأمينية
+
+114
+00:08:57,790 --> 00:09:06,520
+تحتوي على C وH في ال side chain فقطالنانبولان هي
+
+115
+00:09:06,520 --> 00:09:10,420
+الـ amino acid that contains C وH in their side
+
+116
+00:09:10,420 --> 00:09:14,100
+chain لما أجى أطلع على الاري جروب هاي إيش فيها
+
+117
+00:09:14,100 --> 00:09:19,520
+بلاقي فيها بس يا ذرات كربون يا هيدروجين أو كربون
+
+118
+00:09:19,520 --> 00:09:24,470
+وهيدروجينالألاجي في الـ side-chain كربون وهيدروجين
+
+119
+00:09:24,470 --> 00:09:29,050
+فقط، لا تحتوي أي مجموعة قطبية أخرى، وهي غير قادرة
+
+120
+00:09:29,050 --> 00:09:34,250
+على تكوين روابط أيونية ولا هيدروجينية ولا قادرة
+
+121
+00:09:34,250 --> 00:09:39,430
+على كسب أو فقد الإلكترونات، هي الـ non-polar amino
+
+122
+00:09:39,430 --> 00:09:42,930
+acidتكون تحتوي في الـ sided chain على كاربون
+
+123
+00:09:42,930 --> 00:09:47,830
+وهيدروجين فقط غير قادر على تكوين روابط هيدروجينية
+
+124
+00:09:47,830 --> 00:09:54,170
+ولا ايونية ولا قادر على فقد وكسب الالكترونال الجزء
+
+125
+00:09:54,170 --> 00:09:57,730
+التاني من الأحماض الأمينية هي الـ polar القطبية
+
+126
+00:09:57,730 --> 00:10:03,810
+القطبية مقسمة لقسمين في منها بتكون قطبية مشحونة
+
+127
+00:10:03,810 --> 00:10:08,050
+وفي قطبية غير مشحونة يعني في منها polar charged
+
+128
+00:10:08,050 --> 00:10:13,530
+وفي polar unchargedالـ Bowler سواء كانت charged أو
+
+129
+00:10:13,530 --> 00:10:17,850
+uncharged الـ side chain بيكون يحتوي على مجموعة
+
+130
+00:10:17,850 --> 00:10:22,030
+قطبية لو جيتي على الـ R على الـ side chain تطلع
+
+131
+00:10:22,030 --> 00:10:26,850
+عليه بتلاقي يحتوي على مجموعة قطبية إيش هي
+
+132
+00:10:26,850 --> 00:10:30,930
+المجموعات القطبية؟ ممكن تكون OH اللي هي Hydroxyl
+
+133
+00:10:30,930 --> 00:10:36,290
+group هي مجموعة قطبية OH Hydroxyl group معناها C
+
+134
+00:10:36,290 --> 00:10:42,090
+double bond O اللي هي الكربوني ال groupCWO هي
+
+135
+00:10:42,090 --> 00:10:46,190
+الكربوني للجروب فهي عندنا الـ sulfhydryl للجروب
+
+136
+00:10:46,190 --> 00:10:51,130
+الـ sulfhydryl للجروب اللي هي الـSH في عندنا
+
+137
+00:10:51,130 --> 00:10:56,450
+الأمين للجروب اللي هي الـH2 أمين للجروب هدولة كلهم
+
+138
+00:10:56,450 --> 00:11:00,650
+مجموعات قطبيةلو شوفتهم في الـ sided chain بعرف إنه
+
+139
+00:11:00,650 --> 00:11:06,150
+هذا بولا طب هل هو charged ولا uncharged? uncharged
+
+140
+00:11:06,150 --> 00:11:09,110
+يعني ماعلهوش شحنة لا عليه positive ولا عليه
+
+141
+00:11:09,110 --> 00:11:13,170
+negative فيه مجموعة قطبية لكن لا هي positive ولا
+
+142
+00:11:13,170 --> 00:11:17,370
+هي negative طب ليه charged؟ charged معناه إنه
+
+143
+00:11:17,370 --> 00:11:21,470
+يحتوي على مجموعة قطبية بشحنة إما بتكون الشحنة
+
+144
+00:11:21,470 --> 00:11:26,200
+positive لـcharged إذا كانت positive يعني باسأو
+
+145
+00:11:26,200 --> 00:11:32,520
+negative charge يعني أسل، إذا الأحماض الأمنية تقسم
+
+146
+00:11:32,520 --> 00:11:37,400
+حسب ال polarity إلى polar أو non-polar، non-polar
+
+147
+00:11:37,400 --> 00:11:45,790
+لا يحتوي على مجموعة قطبيةوإنما يحتوي على بس كربون
+
+148
+00:11:45,790 --> 00:11:49,450
+وهيدروجين في الـ sided chain طب البولر يحتوي على
+
+149
+00:11:49,450 --> 00:11:55,290
+مجموعة قطبية إما حكينا هيدروكسيل أو كربونيل أو
+
+150
+00:11:55,290 --> 00:12:00,430
+sulfahydride أو أمين إجروم هدول المجموعات القطبية
+
+151
+00:12:00,430 --> 00:12:06,150
+إما تكون uncharged أو charged عليها شحنة أو معلهاش
+
+152
+00:12:06,150 --> 00:12:06,910
+شحنة
+
+153
+00:12:10,230 --> 00:12:13,090
+لو جينا نتفرج على الصور الموجودة هان أو على الـ
+
+154
+00:12:13,090 --> 00:12:17,190
+structure الأحماض الأمنية موجودة هان هذه الصور
+
+155
+00:12:17,190 --> 00:12:21,270
+مقسمة لأحماض الأمنية لـ polar و non-polar في منها
+
+156
+00:12:21,270 --> 00:12:25,310
+charged و في منها uncharged، تمام؟ هلجيت؟ لو جينا
+
+157
+00:12:25,310 --> 00:12:29,890
+على الصورة من فوق لليسار، في عندنا مجموعة، هلجيت؟
+
+158
+00:12:29,890 --> 00:12:33,190
+أنا كيف بتعرف polar ولا non-polar، حاجة هنا؟ نتطلع
+
+159
+00:12:33,190 --> 00:12:35,750
+على الـside chain، على القعر اللي بتكون في الأسفل،
+
+160
+00:12:35,750 --> 00:12:40,190
+هي الملونةلجيت أول حمض أمين لو جينا تطلعنا بنلاقي
+
+161
+00:12:40,190 --> 00:12:43,630
+الـR هي عبارة عن أتش عن هيدروجين حكينا اللي بيكون
+
+162
+00:12:43,630 --> 00:12:47,810
+عنده الـR الـR group عبارة عن هيدروجين أو كربونا
+
+163
+00:12:47,810 --> 00:12:52,400
+أو الاتنين مع بعض بيكون non-polarفهذه المجموعة
+
+164
+00:12:52,400 --> 00:12:57,780
+كلها الجزء اللي فار من اليسار هي non-polar amino
+
+165
+00:12:57,780 --> 00:13:02,980
+acid لأن الـR group تحتوي على كربون وهيدروجين فقط،
+
+166
+00:13:02,980 --> 00:13:08,240
+تمام؟ الجتلة تحت منها على اليسار للأسفل لو طلعنا
+
+167
+00:13:08,240 --> 00:13:14,040
+على الـR group اللي تحت بنلاقيها تحتوي على مجموعة
+
+168
+00:13:14,040 --> 00:13:20,350
+قطبية تحتوي على مجموعة قطبيةفي منها بتحتوي على
+
+169
+00:13:20,350 --> 00:13:24,670
+هيدروكسيل جروب في منها بتحتوي على أمين جروب في
+
+170
+00:13:24,670 --> 00:13:29,770
+منها بتحتوي على صلفهيدرال جروب S-H المهم كلهم في
+
+171
+00:13:29,770 --> 00:13:34,870
+الـR group يحتوي على واحدة من المجموعات القطرية
+
+172
+00:13:35,420 --> 00:13:39,800
+يعني الأول يحتوي OH، التاني OH، التالت الستين
+
+173
+00:13:39,800 --> 00:13:43,440
+يحتوي على SH، self-hydraulic group يعني فيه إنه
+
+174
+00:13:43,440 --> 00:13:47,940
+مجموعات قطبية، يبقى هو polar طب هل عليه شحنة؟ لأ
+
+175
+00:13:47,940 --> 00:13:51,580
+فيش شحنات ولا موجم ولا سائل، إذا هو polar
+
+176
+00:13:51,580 --> 00:13:57,400
+uncharged R groupBowler Uncharged Amino Acid لو
+
+177
+00:13:57,400 --> 00:14:00,480
+جينا على جهة اليمين للأسفل، المجمعتين اللي في
+
+178
+00:14:00,480 --> 00:14:04,480
+الأسفل، بنلاقي إن فيهم في الـR group، في عندهم
+
+179
+00:14:04,480 --> 00:14:11,200
+مجموعات قطبية أغلبهم عندهم أمينوجروب أو Carboxyl
+
+180
+00:14:11,200 --> 00:14:15,650
+groupCarbonyl group Amino group او Carbonyl group
+
+181
+00:14:15,650 --> 00:14:20,050
+هدول مجموعات قطبية يبقى polar لكن عليهم شحنات اللي
+
+182
+00:14:20,050 --> 00:14:23,350
+في الأسفل عليهم شحنة سالمة و اللي في الأعلى عليهم
+
+183
+00:14:23,350 --> 00:14:27,490
+شحنة موجبة فبنسميها negatively charged polar و في
+
+184
+00:14:27,490 --> 00:14:32,090
+positively charged polar أما اللي فاض هدولة
+
+185
+00:14:32,090 --> 00:14:36,790
+aromatic لأنه بيحتووا على حلقة إذا كان عندى حمض
+
+186
+00:14:36,790 --> 00:14:42,340
+أمين يحتو على حلقة بنسميه aromaticلو جينا في هذه
+
+187
+00:14:42,340 --> 00:14:45,520
+الصورة بس عشان نرجع لقى اللي قبل حكيناها عشان
+
+188
+00:14:45,520 --> 00:14:49,900
+تفهموه أكتر لو جيتوا على البرولين البرولين موجود
+
+189
+00:14:49,900 --> 00:14:55,080
+في .. على الجهة اليسار في الأسفل في أن حمض الأمين
+
+190
+00:14:55,080 --> 00:14:59,840
+مكتوب تحته برولين طلعوا على الاري جروب إله بنلاقي
+
+191
+00:14:59,840 --> 00:15:05,170
+إنه الاري جروبعمل حلقة مع الـ Amino group يعني
+
+192
+00:15:05,170 --> 00:15:09,110
+عندك ضارة الكربون في النص و فوق مرتبط فيها كربوكسي
+
+193
+00:15:09,110 --> 00:15:12,950
+لـ group COO و من الجانب ضارة هيدروجين و على
+
+194
+00:15:12,950 --> 00:15:16,510
+الأسفل المفروض أن الـ R group من الجانب الـ Amino
+
+195
+00:15:16,510 --> 00:15:21,060
+groupالـ Amino Group هاي مرتبطة مع الـ R مش حرة زي
+
+196
+00:15:21,060 --> 00:15:24,220
+الباقي الأحماض الأمينية لو طلعت على كل الأحماض
+
+197
+00:15:24,220 --> 00:15:28,760
+الأمينية بنلاقي الـ NH2 هاي أو الـ NH3 Plus اللي
+
+198
+00:15:28,760 --> 00:15:31,920
+هي الـ Amino Group حرة بس مرتبطة مع الكربونة
+
+199
+00:15:31,920 --> 00:15:36,290
+المرجزيةإلا في حالة البروتين، في حالة البرولين
+
+200
+00:15:36,290 --> 00:15:41,370
+مرتبطة مع الكربونة المركزية ومرتبطة كمان مع الـR،
+
+201
+00:15:41,370 --> 00:15:45,770
+مع الـside chain عشان هيك سميناها amino group أو
+
+202
+00:15:45,770 --> 00:15:50,830
+secondary amino group مش primary amino group فهو
+
+203
+00:15:50,830 --> 00:15:52,870
+الحمض الأميني الشاد
+
+204
+00:15:58,770 --> 00:16:03,530
+لمّا نشتغل العمل ان شاء الله راح نسير نكتب تقرير
+
+205
+00:16:03,530 --> 00:16:07,230
+نكتب report تقرير عن المعمل اللي احنا اشتغلناه ده
+
+206
+00:16:07,230 --> 00:16:12,330
+نكتب فيه اسم الفحص، الهدف منه، المبدأ العام، خطوات
+
+207
+00:16:12,330 --> 00:16:17,690
+إجارة التجربة، نكتب النتيجة ونكتب تعليق عليها كمّة
+
diff --git a/PL9fwy3NUQKwZ1Q7zOg1ObgufPMjRI3WY1/hVJVg6dDeBU_raw.json b/PL9fwy3NUQKwZ1Q7zOg1ObgufPMjRI3WY1/hVJVg6dDeBU_raw.json
new file mode 100644
index 0000000000000000000000000000000000000000..09143e0eb1b6f1e2b1020c8eb19d64631657f411
--- /dev/null
+++ b/PL9fwy3NUQKwZ1Q7zOg1ObgufPMjRI3WY1/hVJVg6dDeBU_raw.json
@@ -0,0 +1 @@
+{"segments": [{"id": 1, "seek": 1486, "start": 0.0, "end": 14.86, "text": "هنا على الصورة هذه، على الخط اللي فوق أول شيء، هنا عرفنا الصيغة العامة والـ structure العامة للحمض الأميني إنه بيتكون من جمعة كاربو المركزية مرتبطة بهيدروجين وارتشين وكاربوكسين وامينوبرون", "tokens": [3224, 8315, 15844, 31767, 13063, 3660, 29538, 12399, 15844, 33962, 9566, 13672, 1829, 6156, 30543, 5551, 12610, 44049, 38207, 12399, 34105, 6225, 28480, 8315, 31767, 1829, 17082, 3660, 18863, 10943, 3660, 16070, 39184, 3877, 18863, 10943, 3660, 24976, 35571, 11242, 16247, 2304, 9957, 1829, 36145, 3224, 4724, 36081, 30544, 9154, 10874, 2304, 27884, 9122, 9640, 3555, 2407, 9673, 31747, 11622, 10632, 3714, 43500, 3555, 9566, 3660, 39627, 25708, 2288, 29245, 9957, 4032, 9640, 2655, 8592, 9957, 4032, 4117, 9640, 3555, 2407, 4117, 3794, 9957, 4032, 10943, 9957, 2407, 26890, 11536], "avg_logprob": -0.26270604395604397, "compression_ratio": 1.7230769230769232, "no_speech_prob": 8.386373519897461e-05, "words": [{"start": 0.0, "end": 0.1, "word": "هنا", "probability": 0.45361328125}, {"start": 0.1, "end": 0.28, "word": " على", "probability": 0.640625}, {"start": 0.28, "end": 0.7, "word": " الصورة", "probability": 0.9508463541666666}, {"start": 0.7, "end": 0.92, "word": " هذه،", "probability": 0.4307861328125}, {"start": 0.92, "end": 1.02, "word": " على", "probability": 0.84228515625}, {"start": 1.02, "end": 1.32, "word": " الخط", "probability": 0.98388671875}, {"start": 1.32, "end": 1.5, "word": " اللي", "probability": 0.871337890625}, {"start": 1.5, "end": 1.74, "word": " فوق", "probability": 0.989990234375}, {"start": 1.74, "end": 2.02, "word": " أول", "probability": 0.890380859375}, {"start": 2.02, "end": 2.88, "word": " شيء،", "probability": 0.6411946614583334}, {"start": 2.88, "end": 3.08, "word": " هنا", "probability": 0.310791015625}, {"start": 3.08, "end": 3.4, "word": " عرفنا", "probability": 0.9534505208333334}, {"start": 3.4, "end": 3.84, "word": " الصيغة", "probability": 0.9820556640625}, 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قادر على تصنيحها بيقدر يصنحها مش ضروري أني أتأكد أنها موجودة في وجباتي وعددها 11 Non-essential الجسم قادر على تصنيحها Essential الجسم غير قادر على تصنيحها لازم أتأكد أن أنا أخدتها عن طريق الوجبات اليومية وعددها 9", "tokens": [6027, 39184, 8774, 12, 48143, 5551, 2304, 9957, 2407, 32277, 3215, 6024, 118, 13546, 48812, 2288, 13063, 10632, 25724, 38251, 12174, 18513, 2288, 15844, 6055, 9381, 22653, 5016, 11296, 4724, 1829, 28543, 2288, 7251, 9381, 1863, 5016, 11296, 37893, 48812, 2288, 13063, 1829, 14739, 1829, 5551, 2655, 10721, 4117, 3215, 14739, 11296, 3714, 29245, 23328, 3660, 8978, 49610, 3555, 9307, 1829, 4032, 22488, 3215, 11296, 2975, 8774, 12, 48143, 25724, 38251, 12174, 18513, 2288, 15844, 6055, 9381, 22653, 5016, 11296, 49736, 25724, 38251, 32771, 13546, 12174, 18513, 2288, 15844, 6055, 9381, 22653, 5016, 11296, 5296, 31377, 2304, 5551, 2655, 10721, 4117, 3215, 14739, 41850, 5551, 9778, 3215, 2655, 11296, 18871, 23032, 16572, 4587, 2423, 29245, 3555, 9307, 45595, 20498, 10632, 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على C وH في ال side chain فقط", "tokens": [10721, 12610, 44049, 38207, 6055, 9381, 22653, 5172, 16247, 35571, 46958, 16247, 2304, 9957, 10632, 44945, 16606, 15844, 2423, 39184, 12367, 507, 6055, 10859, 4587, 38251, 30731, 12174, 38251, 9957, 8978, 9154, 11296, 12367, 12174, 9566, 3555, 10632, 8978, 9154, 11296, 2107, 12367, 2107, 12367, 37495, 22653, 32771, 13546, 12174, 9566, 3555, 10632, 37495, 22653, 39896, 16247, 35571, 46958, 16247, 2304, 9957, 10632, 6055, 33753, 45865, 15844, 383, 4032, 39, 8978, 2423, 1252, 5021, 6156, 47432], "avg_logprob": -0.16274350339716132, "compression_ratio": 1.7247191011235956, "no_speech_prob": 9.5367431640625e-07, "words": [{"start": 522.99, "end": 523.37, "word": "أول", "probability": 0.97412109375}, {"start": 523.37, "end": 523.59, "word": " شيء", "probability": 0.3636474609375}, {"start": 523.59, "end": 524.01, "word": " تصنيف", "probability": 0.9248046875}, {"start": 524.01, "end": 524.47, "word": " الأحماض", "probability": 0.8987630208333334}, {"start": 524.47, "end": 525.01, "word": " الأمينية", "probability": 0.91357421875}, {"start": 525.01, "end": 525.37, "word": " بناء", "probability": 0.899658203125}, {"start": 525.37, "end": 525.59, "word": " على", "probability": 0.52001953125}, {"start": 525.59, "end": 525.79, "word": " الـ", "probability": 0.828125}, {"start": 525.79, "end": 526.71, "word": " polarity", "probability": 0.6337890625}, {"start": 526.71, "end": 527.29, "word": " تُقسم", "probability": 0.83349609375}, {"start": 527.29, "end": 527.53, "word": " إلى", "probability": 0.96728515625}, {"start": 527.53, "end": 528.19, "word": " قسمين", "probability": 0.9070638020833334}, {"start": 528.19, "end": 528.73, "word": " في", "probability": 0.72412109375}, {"start": 528.73, "end": 529.19, "word": " منها", "probability": 0.97119140625}, {"start": 529.19, "end": 529.63, "word": " polar", "probability": 0.80322265625}, {"start": 529.63, "end": 530.41, "word": " قطبية", "probability": 0.9385986328125}, {"start": 530.41, 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acid", "probability": 0.783203125}, {"start": 547.8, "end": 548.08, "word": " that", "probability": 0.38671875}, {"start": 548.08, "end": 548.66, "word": " contains", "probability": 0.53076171875}, {"start": 548.66, "end": 549.08, "word": " C", "probability": 0.81103515625}, {"start": 549.08, "end": 549.62, "word": " وH", "probability": 0.743896484375}, {"start": 549.62, "end": 549.86, "word": " in", "probability": 0.72509765625}, {"start": 549.86, "end": 550.12, "word": " their", "probability": 0.92431640625}, {"start": 550.12, "end": 550.42, "word": " side", "probability": 0.689453125}, {"start": 550.42, "end": 550.82, "word": " chain", "probability": 0.74560546875}, {"start": 550.82, "end": 551.54, "word": " لما", "probability": 0.824462890625}, {"start": 551.54, "end": 551.78, "word": " أجى", "probability": 0.6490885416666666}, {"start": 551.78, "end": 552.24, "word": " أطلع", "probability": 0.8204345703125}, {"start": 552.24, "end": 552.42, "word": " على", "probability": 0.84765625}, {"start": 552.42, "end": 552.88, "word": " الاري", "probability": 0.6455891927083334}, {"start": 552.88, "end": 553.14, "word": " جروب", "probability": 0.73779296875}, {"start": 553.14, "end": 553.36, "word": " هاي", "probability": 0.684814453125}, {"start": 553.36, "end": 553.6, "word": " إيش", "probability": 0.8221028645833334}, {"start": 553.6, "end": 554.1, "word": " فيها", "probability": 0.974853515625}, {"start": 554.1, "end": 555.32, "word": " بلاقي", "probability": 0.8974609375}, {"start": 555.32, "end": 555.64, "word": " فيها", "probability": 0.9609375}, {"start": 555.64, "end": 555.96, "word": " بس", "probability": 0.9814453125}, {"start": 555.96, "end": 556.18, "word": " يا", "probability": 0.76611328125}, {"start": 556.18, "end": 556.64, "word": " ذرات", "probability": 0.9303385416666666}, {"start": 556.64, "end": 557.24, "word": " كربون", "probability": 0.9444986979166666}, {"start": 557.24, "end": 557.84, "word": " يا", "probability": 0.748046875}, {"start": 557.84, "end": 558.68, "word": " هيدروجين", "probability": 0.93095703125}, {"start": 558.68, "end": 559.0, "word": " أو", "probability": 0.83642578125}, {"start": 559.0, "end": 559.52, "word": " كربون", "probability": 0.9861653645833334}, {"start": 559.52, "end": 560.22, "word": " وهيدروجين", "probability": 0.915771484375}], "temperature": 1.0}, {"id": 26, "seek": 57992, "start": 561.55, "end": 579.93, "text": "الألاجي في الـ side-chain كربون وهيدروجين فقط، لا تحتوي أي مجموعة قطبية أخرى، وهي غير قادرة على تكوين روابط أيونية ولا هيدروجينية ولا قادرة على كسب أو فقد الإلكترونات، هي الـ non-polar amino acid", "tokens": [6027, 10721, 1211, 26108, 1829, 8978, 2423, 39184, 1252, 12, 11509, 9122, 25513, 11536, 4032, 3224, 25708, 2288, 29245, 9957, 6156, 47432, 12399, 20193, 6055, 33753, 45865, 36632, 3714, 7435, 2304, 45367, 3660, 12174, 9566, 3555, 10632, 5551, 34740, 7578, 12399, 37037, 1829, 32771, 13546, 12174, 18513, 25720, 15844, 6055, 4117, 2407, 9957, 12602, 14407, 3555, 9566, 36632, 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"probability": 0.699951171875}, {"start": 601.39, "end": 602.67, "word": " قطبية", "probability": 0.9803466796875}, {"start": 602.67, "end": 603.81, "word": " مشحونة", "probability": 0.9613037109375}, {"start": 603.81, "end": 604.23, "word": " وفي", "probability": 0.765625}, {"start": 604.23, "end": 604.95, "word": " قطبية", "probability": 0.989013671875}, {"start": 604.95, "end": 605.31, "word": " غير", "probability": 0.996826171875}, {"start": 605.31, "end": 606.07, "word": " مشحونة", "probability": 0.982421875}, {"start": 606.07, "end": 606.49, "word": " يعني", "probability": 0.848876953125}, {"start": 606.49, "end": 606.61, "word": " في", "probability": 0.95458984375}, {"start": 606.61, "end": 606.89, "word": " منها", "probability": 0.99560546875}, {"start": 606.89, "end": 607.29, "word": " polar", "probability": 0.83837890625}, {"start": 607.29, "end": 608.05, "word": " charged", "probability": 0.91162109375}, {"start": 608.05, "end": 608.43, "word": " وفي", "probability": 0.900634765625}, {"start": 608.43, "end": 608.87, "word": " polar", "probability": 0.96875}, {"start": 608.87, "end": 609.69, "word": " uncharged", "probability": 0.779296875}], "temperature": 1.0}, {"id": 28, "seek": 63903, "start": 610.79, "end": 639.03, "text": "الـ Bowler سواء كانت charged أو uncharged الـ side chain بيكون يحتوي على مجموعة قطبية لو جيتي على الـ R على الـ side chain تطلع عليه بتلاقي يحتوي على مجموعة قطبية إيش هي المجموعات القطبية؟ ممكن تكون OH اللي هي Hydroxyl group هي مجموعة قطبية OH Hydroxyl group معناها C double bond O اللي هي الكربوني ال group", "tokens": [6027, 39184, 12903, 1918, 8608, 2407, 16606, 25961, 2655, 11109, 34051, 33686, 289, 3004, 2423, 39184, 1252, 5021, 4724, 1829, 30544, 7251, 33753, 45865, 15844, 3714, 7435, 2304, 2407, 27884, 12174, 9566, 3555, 10632, 45164, 10874, 1829, 31371, 15844, 2423, 39184, 497, 15844, 2423, 39184, 1252, 5021, 6055, 9566, 1211, 3615, 47356, 39894, 15040, 38436, 7251, 33753, 45865, 15844, 3714, 7435, 2304, 2407, 27884, 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0.8193359375}, {"start": 613.53, "end": 614.31, "word": " uncharged", "probability": 0.81201171875}, {"start": 614.31, "end": 614.75, "word": " الـ", "probability": 0.789794921875}, {"start": 614.75, "end": 614.93, "word": " side", "probability": 0.399169921875}, {"start": 614.93, "end": 615.57, "word": " chain", "probability": 0.6787109375}, {"start": 615.57, "end": 616.17, "word": " بيكون", "probability": 0.7810872395833334}, {"start": 616.17, "end": 616.81, "word": " يحتوي", "probability": 0.98583984375}, {"start": 616.81, "end": 617.09, "word": " على", "probability": 0.90087890625}, {"start": 617.09, "end": 617.85, "word": " مجموعة", "probability": 0.94736328125}, {"start": 617.85, "end": 618.61, "word": " قطبية", "probability": 0.9788818359375}, {"start": 618.61, "end": 619.47, "word": " لو", "probability": 0.82958984375}, {"start": 619.47, "end": 619.75, "word": " جيتي", "probability": 0.8723958333333334}, {"start": 619.75, "end": 619.97, "word": " على", "probability": 0.8876953125}, {"start": 619.97, "end": 620.15, "word": " الـ", "probability": 0.6739501953125}, {"start": 620.15, "end": 620.35, "word": " R", "probability": 0.494873046875}, {"start": 620.35, "end": 620.53, "word": " على", "probability": 0.390380859375}, {"start": 620.53, "end": 620.61, "word": " الـ", "probability": 0.892578125}, {"start": 620.61, "end": 620.87, "word": " side", "probability": 0.798828125}, {"start": 620.87, "end": 621.39, "word": " chain", "probability": 0.88427734375}, {"start": 621.39, "end": 622.03, "word": " تطلع", "probability": 0.8798828125}, {"start": 622.03, "end": 622.31, "word": " عليه", "probability": 0.9853515625}, {"start": 622.31, "end": 622.75, "word": " بتلاقي", "probability": 0.801513671875}, {"start": 622.75, "end": 623.39, "word": " يحتوي", "probability": 0.9371744791666666}, {"start": 623.39, "end": 623.73, "word": " على", "probability": 0.9208984375}, {"start": 623.73, "end": 624.67, "word": " مجموعة", "probability": 0.94287109375}, {"start": 624.67, "end": 625.45, "word": " قطبية", "probability": 0.9927978515625}, {"start": 625.45, "end": 626.67, "word": " إيش", "probability": 0.7559407552083334}, {"start": 626.67, "end": 626.85, "word": " هي", "probability": 0.91845703125}, {"start": 626.85, "end": 627.39, "word": " المجموعات", "probability": 0.953125}, {"start": 627.39, "end": 628.63, "word": " القطبية؟", "probability": 0.97041015625}, {"start": 628.63, "end": 628.87, "word": " ممكن", "probability": 0.976318359375}, {"start": 628.87, "end": 629.19, "word": " تكون", "probability": 0.99267578125}, {"start": 629.19, "end": 629.81, "word": " OH", "probability": 0.78857421875}, {"start": 629.81, "end": 630.03, "word": " اللي", "probability": 0.70849609375}, {"start": 630.03, "end": 630.17, "word": " هي", "probability": 0.931640625}, {"start": 630.17, "end": 630.93, "word": " Hydroxyl", "probability": 0.578857421875}, {"start": 630.93, "end": 631.29, "word": " group", "probability": 0.70458984375}, {"start": 631.29, "end": 631.59, "word": " هي", "probability": 0.43212890625}, {"start": 631.59, "end": 632.01, "word": " مجموعة", "probability": 0.95}, {"start": 632.01, "end": 632.57, "word": " قطبية", "probability": 0.9927978515625}, {"start": 632.57, "end": 633.39, "word": " OH", "probability": 0.779296875}, {"start": 633.39, "end": 634.57, "word": " Hydroxyl", "probability": 0.75323486328125}, {"start": 634.57, "end": 634.83, "word": " group", "probability": 0.93359375}, {"start": 634.83, "end": 635.37, "word": " معناها", "probability": 0.9524739583333334}, {"start": 635.37, "end": 636.29, "word": " C", "probability": 0.75634765625}, {"start": 636.29, "end": 636.67, "word": " double", "probability": 0.6318359375}, {"start": 636.67, "end": 637.13, "word": " bond", "probability": 0.8798828125}, {"start": 637.13, "end": 637.49, "word": " O", "probability": 0.9462890625}, {"start": 637.49, "end": 637.73, "word": " اللي", "probability": 0.9638671875}, {"start": 637.73, "end": 637.85, "word": " هي", "probability": 0.90673828125}, {"start": 637.85, "end": 638.57, "word": " الكربوني", "probability": 0.9154052734375}, {"start": 638.57, "end": 638.73, "word": " ال", "probability": 0.1259765625}, {"start": 638.73, "end": 639.03, "word": " group", "probability": 0.86279296875}], "temperature": 1.0}, {"id": 29, "seek": 65757, "start": 640.39, "end": 657.57, "text": "CWO هي الكربوني للجروب فهي عندنا الـ sulfhydryl للجروب الـ sulfhydryl للجروب اللي هي الـSH في عندنا الأمين للجروب اللي هي الـH2 أمين للجروب هدولة كلهم مجموعات قطبية", "tokens": [34, 54, 46, 39896, 33251, 25513, 11536, 1829, 24976, 7435, 32887, 3555, 6156, 3224, 1829, 43242, 8315, 2423, 39184, 22925, 21591, 627, 75, 24976, 7435, 32887, 3555, 2423, 39184, 22925, 21591, 627, 75, 24976, 7435, 32887, 3555, 13672, 1829, 39896, 2423, 39184, 17308, 8978, 43242, 8315, 16247, 2304, 9957, 24976, 7435, 32887, 3555, 13672, 1829, 39896, 2423, 39184, 39, 17, 5551, 2304, 9957, 24976, 7435, 32887, 3555, 8032, 3215, 12610, 3660, 28242, 16095, 3714, 7435, 2304, 45367, 9307, 12174, 9566, 3555, 10632], "avg_logprob": -0.32981928644410097, "compression_ratio": 1.8639455782312926, "no_speech_prob": 9.894371032714844e-06, "words": [{"start": 640.39, "end": 641.53, "word": "CWO", "probability": 0.4892985026041667}, {"start": 641.53, "end": 642.09, "word": " هي", "probability": 0.2392578125}, {"start": 642.09, "end": 642.95, "word": " الكربوني", "probability": 0.802978515625}, {"start": 642.95, "end": 643.47, "word": " للجروب", "probability": 0.72979736328125}, {"start": 643.47, "end": 644.13, "word": " فهي", "probability": 0.6759033203125}, {"start": 644.13, "end": 644.39, "word": " عندنا", "probability": 0.6934814453125}, {"start": 644.39, "end": 644.55, "word": " الـ", "probability": 0.7783203125}, {"start": 644.55, "end": 645.51, "word": " sulfhydryl", "probability": 0.607025146484375}, {"start": 645.51, "end": 646.19, "word": " للجروب", "probability": 0.80706787109375}, {"start": 646.19, "end": 646.67, "word": " الـ", "probability": 0.558349609375}, {"start": 646.67, "end": 647.89, "word": " sulfhydryl", "probability": 0.772064208984375}, {"start": 647.89, "end": 648.43, "word": " للجروب", "probability": 0.8956298828125}, {"start": 648.43, "end": 648.59, "word": " اللي", "probability": 0.822998046875}, {"start": 648.59, "end": 648.77, "word": " هي", "probability": 0.7216796875}, {"start": 648.77, "end": 649.51, "word": " الـSH", "probability": 0.69384765625}, {"start": 649.51, "end": 650.69, "word": " في", "probability": 0.407470703125}, {"start": 650.69, "end": 651.13, "word": " عندنا", "probability": 0.94775390625}, {"start": 651.13, "end": 651.73, "word": " الأمين", "probability": 0.707763671875}, {"start": 651.73, "end": 652.39, "word": " للجروب", "probability": 0.82000732421875}, {"start": 652.39, "end": 652.91, "word": " اللي", "probability": 0.8623046875}, {"start": 652.91, "end": 653.05, "word": " هي", "probability": 0.6494140625}, {"start": 653.05, "end": 654.13, "word": " الـH2", "probability": 0.9268798828125}, {"start": 654.13, "end": 655.07, "word": " أمين", "probability": 0.7456868489583334}, {"start": 655.07, "end": 655.65, "word": " للجروب", "probability": 0.95849609375}, {"start": 655.65, "end": 656.21, "word": " هدولة", "probability": 0.832763671875}, {"start": 656.21, "end": 656.45, "word": " كلهم", "probability": 0.922119140625}, {"start": 656.45, "end": 657.07, "word": " مجموعات", "probability": 0.9796875}, {"start": 657.07, "end": 657.57, "word": " قطبية", "probability": 0.9520263671875}], "temperature": 1.0}, {"id": 30, "seek": 68521, "start": 657.85, "end": 685.21, "text": "لو شوفتهم في الـ sided chain بعرف إنه هذا بولا طب هل هو charged ولا uncharged? uncharged يعني ماعلهوش شحنة لا عليه positive ولا عليه negative فيه مجموعة قطبية لكن لا هي positive ولا هي negative طب ليه charged؟ charged معناه إنه يحتوي على مجموعة قطبية بشحنة إما بتكون الشحنة positive لـcharged إذا كانت positive يعني باس", "tokens": [1211, 2407, 13412, 38688, 2655, 16095, 8978, 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Amino", "probability": 0.856689453125}, {"start": 916.51, "end": 916.77, "word": " group", "probability": 0.9619140625}], "temperature": 1.0}, {"id": 42, "seek": 93262, "start": 917.32, "end": 932.62, "text": "الـ Amino Group هاي مرتبطة مع الـ R مش حرة زي الباقي الأحماض الأمينية لو طلعت على كل الأحماض الأمينية بنلاقي الـ NH2 هاي أو الـ NH3 Plus اللي هي الـ Amino Group حرة بس مرتبطة مع الكربونة المرجزية", "tokens": [6027, 39184, 2012, 2982, 10500, 8032, 47302, 3714, 43500, 3555, 9566, 3660, 20449, 2423, 39184, 497, 37893, 11331, 25720, 30767, 1829, 29739, 995, 38436, 16247, 35571, 46958, 16247, 2304, 9957, 10632, 45164, 23032, 1211, 34268, 15844, 28242, 16247, 35571, 46958, 16247, 2304, 9957, 10632, 44945, 15040, 38436, 2423, 39184, 31118, 17, 8032, 47302, 34051, 2423, 39184, 31118, 18, 7721, 13672, 1829, 39896, 2423, 39184, 2012, 2982, 10500, 11331, 25720, 4724, 3794, 3714, 43500, 3555, 9566, 3660, 20449, 33251, 25513, 11536, 3660, 9673, 47341, 11622, 10632], "avg_logprob": -0.13535610118577646, "compression_ratio": 1.8448275862068966, "no_speech_prob": 1.430511474609375e-06, "words": [{"start": 917.32, "end": 917.54, "word": "الـ", "probability": 0.968994140625}, {"start": 917.54, "end": 917.9, "word": " Amino", "probability": 0.85009765625}, {"start": 917.9, "end": 918.22, "word": " Group", "probability": 0.439697265625}, {"start": 918.22, "end": 918.66, "word": " هاي", "probability": 0.6239013671875}, {"start": 918.66, "end": 919.5, "word": " مرتبطة", "probability": 0.98291015625}, {"start": 919.5, "end": 919.68, "word": " مع", "probability": 0.98779296875}, {"start": 919.68, "end": 919.92, "word": " الـ", "probability": 0.980224609375}, {"start": 919.92, "end": 920.16, "word": " R", "probability": 0.73193359375}, {"start": 920.16, "end": 920.38, "word": " مش", "probability": 0.744140625}, {"start": 920.38, "end": 920.76, "word": " حرة", "probability": 0.81591796875}, {"start": 920.76, "end": 921.06, "word": " زي", "probability": 0.953125}, {"start": 921.06, "end": 921.48, "word": " الباقي", "probability": 0.716796875}, {"start": 921.48, "end": 921.86, "word": " الأحماض", "probability": 0.86474609375}, {"start": 921.86, "end": 922.38, "word": " الأمينية", "probability": 0.8118896484375}, {"start": 922.38, "end": 922.9, "word": " لو", "probability": 0.76904296875}, {"start": 922.9, "end": 923.3, "word": " طلعت", "probability": 0.99365234375}, {"start": 923.3, "end": 923.48, "word": " على", "probability": 0.921875}, {"start": 923.48, "end": 923.7, "word": " كل", "probability": 0.97802734375}, {"start": 923.7, "end": 924.22, "word": " الأحماض", "probability": 0.9864908854166666}, {"start": 924.22, "end": 924.72, "word": " الأمينية", "probability": 0.988037109375}, {"start": 924.72, "end": 925.16, "word": " بنلاقي", "probability": 0.8212890625}, {"start": 925.16, "end": 925.34, "word": " الـ", "probability": 0.96728515625}, {"start": 925.34, "end": 926.24, "word": " NH2", "probability": 0.867431640625}, {"start": 926.24, "end": 927.0, "word": " هاي", "probability": 0.75390625}, {"start": 927.0, "end": 927.26, "word": " أو", "probability": 0.96923828125}, {"start": 927.26, "end": 927.54, "word": " الـ", "probability": 0.964111328125}, {"start": 927.54, "end": 928.22, "word": " NH3", "probability": 0.958740234375}, {"start": 928.22, "end": 928.6, "word": " Plus", "probability": 0.1241455078125}, {"start": 928.6, "end": 928.76, "word": " اللي", "probability": 0.87646484375}, {"start": 928.76, "end": 928.88, "word": " هي", "probability": 0.8466796875}, {"start": 928.88, "end": 928.96, "word": " الـ", "probability": 0.89794921875}, {"start": 928.96, "end": 929.28, "word": " Amino", "probability": 0.86767578125}, {"start": 929.28, "end": 929.52, "word": " Group", "probability": 0.88916015625}, {"start": 929.52, "end": 929.96, "word": " حرة", "probability": 0.968505859375}, {"start": 929.96, "end": 930.46, "word": " بس", "probability": 0.96728515625}, {"start": 930.46, "end": 931.14, "word": " مرتبطة", "probability": 0.99453125}, {"start": 931.14, "end": 931.26, "word": " مع", "probability": 0.99462890625}, {"start": 931.26, "end": 931.92, "word": " الكربونة", "probability": 0.8826904296875}, {"start": 931.92, "end": 932.62, "word": " المرجزية", "probability": 0.8662109375}], "temperature": 1.0}, {"id": 43, "seek": 95287, "start": 933.03, "end": 952.87, "text": "إلا في حالة البروتين، في حالة البرولين مرتبطة مع الكربونة المركزية ومرتبطة كمان مع الـR، مع الـside chain عشان هيك سميناها amino group أو secondary amino group مش primary amino group فهو الحمض الأميني الشاد", "tokens": [28814, 15040, 8978, 11331, 6027, 3660, 2423, 26890, 35473, 9957, 12399, 8978, 11331, 6027, 3660, 2423, 26890, 12610, 9957, 3714, 43500, 3555, 9566, 3660, 20449, 33251, 25513, 11536, 3660, 9673, 31747, 11622, 10632, 4032, 2304, 43500, 3555, 9566, 3660, 9122, 2304, 7649, 20449, 2423, 39184, 49, 12399, 20449, 2423, 39184, 1812, 5021, 6225, 8592, 7649, 39896, 4117, 8608, 2304, 1829, 8315, 11296, 24674, 1594, 34051, 11396, 24674, 1594, 37893, 6194, 24674, 1594, 6156, 3224, 2407, 21542, 2304, 11242, 16247, 2304, 9957, 1829, 25124, 18513], "avg_logprob": -0.12334558893652524, "compression_ratio": 1.7037037037037037, "no_speech_prob": 5.245208740234375e-06, "words": [{"start": 933.03, "end": 933.45, "word": "إلا", "probability": 0.84033203125}, {"start": 933.45, "end": 933.61, "word": " في", "probability": 0.94775390625}, {"start": 933.61, "end": 934.01, "word": " حالة", "probability": 0.9973958333333334}, {"start": 934.01, "end": 935.01, "word": " البروتين،", "probability": 0.791357421875}, {"start": 935.01, "end": 935.13, "word": " في", "probability": 0.92529296875}, {"start": 935.13, "end": 935.55, "word": " حالة", "probability": 0.99853515625}, {"start": 935.55, "end": 936.29, "word": " البرولين", "probability": 0.9610595703125}, {"start": 936.29, "end": 937.31, "word": " مرتبطة", "probability": 0.9400390625}, {"start": 937.31, "end": 937.43, "word": " مع", "probability": 0.97509765625}, {"start": 937.43, "end": 938.13, "word": " الكربونة", "probability": 0.868408203125}, {"start": 938.13, "end": 938.93, "word": " المركزية", "probability": 0.9893798828125}, {"start": 938.93, "end": 940.07, "word": " ومرتبطة", "probability": 0.919189453125}, {"start": 940.07, "end": 940.53, "word": " كمان", "probability": 0.9319661458333334}, {"start": 940.53, "end": 940.79, "word": " مع", "probability": 0.96484375}, {"start": 940.79, "end": 941.37, "word": " الـR،", "probability": 0.75}, {"start": 941.37, "end": 941.43, "word": " مع", "probability": 0.9326171875}, {"start": 941.43, "end": 941.83, "word": " الـside", "probability": 0.7430826822916666}, {"start": 941.83, "end": 942.31, "word": " chain", "probability": 0.69775390625}, {"start": 942.31, "end": 943.03, "word": " عشان", "probability": 0.94775390625}, {"start": 943.03, "end": 943.23, "word": " هيك", "probability": 0.898681640625}, {"start": 943.23, "end": 944.03, "word": " سميناها", "probability": 0.87880859375}, {"start": 944.03, "end": 944.63, "word": " amino", "probability": 0.47412109375}, {"start": 944.63, "end": 945.21, "word": " group", "probability": 0.9716796875}, {"start": 945.21, "end": 945.77, "word": " أو", "probability": 0.8974609375}, {"start": 945.77, "end": 946.49, "word": " secondary", "probability": 0.96826171875}, {"start": 946.49, "end": 947.09, "word": " amino", "probability": 0.98779296875}, {"start": 947.09, "end": 947.61, "word": " group", "probability": 0.98388671875}, {"start": 947.61, "end": 947.91, "word": " مش", "probability": 0.7685546875}, {"start": 947.91, "end": 948.73, "word": " primary", "probability": 0.583984375}, {"start": 948.73, "end": 949.61, "word": " amino", "probability": 0.986328125}, {"start": 949.61, "end": 950.33, "word": " group", "probability": 0.982421875}, {"start": 950.33, "end": 950.83, "word": " فهو", "probability": 0.9462890625}, {"start": 950.83, "end": 951.23, "word": " الحمض", "probability": 0.95849609375}, {"start": 951.23, "end": 951.99, "word": " الأميني", "probability": 0.9398193359375}, {"start": 951.99, "end": 952.87, "word": " الشاد", "probability": 0.847900390625}], "temperature": 1.0}, {"id": 44, "seek": 97769, "start": 958.77, "end": 977.69, "text": "لمّا نشتغل العمل ان شاء الله راح نسير نكتب تقرير نكتب report تقرير عن المعمل اللي احنا اشتغلناه ده نكتب فيه اسم الفحص، الهدف منه، المبدأ العام، خطوات إجارة التجربة، نكتب النتيجة ونكتب تعليق عليها كمّة", "tokens": [19528, 11703, 995, 8717, 8592, 2655, 17082, 1211, 18863, 42213, 16472, 13412, 16606, 21984, 12602, 39319, 8717, 3794, 13546, 8717, 4117, 2655, 3555, 6055, 4587, 2288, 13546, 8717, 4117, 2655, 3555, 2275, 6055, 4587, 2288, 13546, 18871, 9673, 25957, 1211, 13672, 1829, 1975, 5016, 8315, 1975, 8592, 2655, 17082, 1211, 8315, 3224, 11778, 3224, 8717, 4117, 2655, 3555, 8978, 3224, 24525, 2304, 27188, 5016, 9381, 12399, 2423, 3224, 3215, 5172, 9154, 3224, 12399, 9673, 44510, 10721, 18863, 10943, 12399, 16490, 9566, 2407, 9307, 11933, 7435, 9640, 3660, 16712, 7435, 25513, 3660, 12399, 8717, 4117, 2655, 3555, 28239, 31371, 7435, 3660, 4032, 1863, 4117, 2655, 3555, 37279, 20292, 4587, 25894, 11296, 9122, 2304, 11703, 3660], "avg_logprob": -0.11209239208179972, "compression_ratio": 1.8223350253807107, "no_speech_prob": 3.4570693969726562e-06, "words": [{"start": 958.77, "end": 959.13, "word": "لمّا", "probability": 0.8209635416666666}, {"start": 959.13, "end": 959.67, "word": " نشتغل", "probability": 0.98779296875}, {"start": 959.67, "end": 960.07, "word": " العمل", "probability": 0.96484375}, {"start": 960.07, "end": 960.29, "word": " ان", "probability": 0.46337890625}, {"start": 960.29, "end": 960.53, "word": " شاء", "probability": 0.988525390625}, {"start": 960.53, "end": 960.87, "word": " الله", "probability": 0.962890625}, {"start": 960.87, "end": 961.55, "word": " راح", "probability": 0.778076171875}, {"start": 961.55, "end": 962.17, "word": " نسير", "probability": 0.8665364583333334}, {"start": 962.17, "end": 962.99, "word": " نكتب", "probability": 0.9482421875}, {"start": 962.99, "end": 963.53, "word": " تقرير", "probability": 0.944091796875}, {"start": 963.53, "end": 964.27, "word": " نكتب", "probability": 0.90869140625}, {"start": 964.27, "end": 964.69, "word": " report", "probability": 0.9501953125}, {"start": 964.69, "end": 965.23, "word": " تقرير", "probability": 0.88916015625}, {"start": 965.23, "end": 965.43, "word": " عن", "probability": 0.9921875}, {"start": 965.43, "end": 965.83, "word": " المعمل", "probability": 0.9720052083333334}, {"start": 965.83, "end": 965.95, "word": " اللي", "probability": 0.927001953125}, {"start": 965.95, "end": 966.09, "word": " احنا", "probability": 0.8689778645833334}, {"start": 966.09, "end": 966.95, "word": " اشتغلناه", "probability": 0.947265625}, {"start": 966.95, "end": 967.23, "word": " ده", "probability": 0.754638671875}, {"start": 967.23, "end": 967.57, "word": " نكتب", "probability": 0.9962158203125}, {"start": 967.57, "end": 967.79, "word": " فيه", "probability": 0.97900390625}, {"start": 967.79, "end": 968.09, "word": " اسم", "probability": 0.945068359375}, {"start": 968.09, "end": 969.15, "word": " الفحص،", "probability": 0.82568359375}, {"start": 969.15, "end": 969.55, "word": " الهدف", "probability": 0.990478515625}, {"start": 969.55, "end": 970.37, "word": " منه،", "probability": 0.9786783854166666}, {"start": 970.37, "end": 970.87, "word": " المبدأ", "probability": 0.9440104166666666}, {"start": 970.87, "end": 971.81, "word": " العام،", "probability": 0.9109700520833334}, {"start": 971.81, "end": 972.33, "word": " خطوات", "probability": 0.96630859375}, {"start": 972.33, "end": 972.73, "word": " إجارة", "probability": 0.81695556640625}, {"start": 972.73, "end": 973.59, "word": " التجربة،", "probability": 0.948046875}, {"start": 973.59, "end": 974.11, "word": " نكتب", "probability": 0.998291015625}, {"start": 974.11, "end": 974.89, "word": " النتيجة", "probability": 0.97314453125}, {"start": 974.89, "end": 975.91, "word": " ونكتب", "probability": 0.83017578125}, {"start": 975.91, "end": 976.69, "word": " تعليق", "probability": 0.98828125}, {"start": 976.69, "end": 977.03, "word": " عليها", "probability": 0.817138671875}, {"start": 977.03, "end": 977.69, "word": " كمّة", "probability": 0.670135498046875}], "temperature": 1.0}], "language": "ar", "language_probability": 1.0, "duration": 984.41, "duration_after_vad": 946.2615937500011}
\ No newline at end of file
diff --git a/PL9fwy3NUQKwZ1Q7zOg1ObgufPMjRI3WY1/iXxjfqDroRk.srt b/PL9fwy3NUQKwZ1Q7zOg1ObgufPMjRI3WY1/iXxjfqDroRk.srt
new file mode 100644
index 0000000000000000000000000000000000000000..435c08614af36711aeb5789fc7f6558aa5f3fe63
--- /dev/null
+++ b/PL9fwy3NUQKwZ1Q7zOg1ObgufPMjRI3WY1/iXxjfqDroRk.srt
@@ -0,0 +1,839 @@
+1
+00:00:05,480 --> 00:00:09,280
+أول فحص هو الـ Ninhydrin الـ Ninhydrin إيش ال
+
+2
+00:00:09,280 --> 00:00:12,700
+objective لو فحصتش الهدف من الفحص to detect the
+
+3
+00:00:12,700 --> 00:00:16,300
+presence of a free alpha amino group بيدور على ال
+
+4
+00:00:16,300 --> 00:00:20,680
+free alpha amino group إذا موجود free alpha amino
+
+5
+00:00:20,680 --> 00:00:25,180
+group هيتغير اللون، بيعطيني اللون purple في حالات
+
+6
+00:00:25,180 --> 00:00:28,800
+اللي موجودة في free alpha amino group مين عنده فيه
+
+7
+00:00:28,800 --> 00:00:31,740
+Alpha Amino Group؟ إذا ذاكرين الحمض الأميني، اعيش
+
+8
+00:00:31,740 --> 00:00:35,040
+الصيغة العامة له، تكون من Center Carbon ومن
+
+9
+00:00:35,040 --> 00:00:38,640
+الهيدروجينة، من ال Side Chain ومن Carboxyl Group
+
+10
+00:00:38,640 --> 00:00:43,160
+ومن Amino Group Amino Group هاي سميناها Primary
+
+11
+00:00:43,160 --> 00:00:47,720
+Amino Group أو Alpha Amino Group، موجودة في حالة
+
+12
+00:00:47,720 --> 00:00:51,920
+البرولين سميناها Amino Groupيعني الأحماض الأمينية
+
+13
+00:00:51,920 --> 00:00:57,380
+كلها تحتوي على free alpha amino group ما عدا
+
+14
+00:00:57,380 --> 00:01:01,600
+البرولين عنده amino group يعني مين هيعطيني positive
+
+15
+00:01:01,600 --> 00:01:04,880
+مع هذا الفحص لو أنا اشتغلت مجموعة أحماض أمينية على
+
+16
+00:01:04,880 --> 00:01:08,400
+هذا الفحص؟ كلهم هيعطوني positive لأنهم كلهم بيحتوي
+
+17
+00:01:08,400 --> 00:01:13,800
+على free alpha amino group تمام؟ طيب، لذا ال
+
+18
+00:01:13,800 --> 00:01:16,260
+objective to detect the presence of free alpha
+
+19
+00:01:16,260 --> 00:01:19,360
+amino group هل موجود free alpha amino group أو لا؟
+
+20
+00:01:19,360 --> 00:01:21,940
+إذا تغير اللون يعني موجود، إذا ما تغيرش يعني مش
+
+21
+00:01:21,940 --> 00:01:25,680
+موجود طيب إيش ال principle؟ مبدأ العمل للفحص؟ كيف
+
+22
+00:01:25,680 --> 00:01:29,120
+يشتغل الفحص؟ ال principle لو نعملها على نظام
+
+23
+00:01:29,120 --> 00:01:32,320
+معادلات بيسهل عليه كيف هي هذا الفحص هو الـ
+
+24
+00:01:32,320 --> 00:01:35,840
+principle أنه عندنا Amino Acid أنا بضيف حمض أميني
+
+25
+00:01:35,840 --> 00:01:38,980
+بضيف عليه Ninhydrin Reagent إيش بيعمل
+
+26
+00:01:38,980 --> 00:01:43,200
+الـ Ninhydrin Reagent؟ بيشتغل على الـ free amino group
+
+27
+00:01:43,200 --> 00:01:47,060
+بيعطيني NH3 لحالها أنا بشتريها وبيعطيني
+
+28
+00:01:47,060 --> 00:01:51,120
+Hydrindantin إذا الأمينو أسد بضيف عليه نينهيدرين
+
+29
+00:01:51,120 --> 00:01:55,300
+بيعطيني NH3 و Hydrindantin مركب اسمه
+
+30
+00:01:55,300 --> 00:02:00,960
+Hydrindantin نجيب الـ NH3 والـ Hydrindantin 
+
+31
+00:02:00,960 --> 00:02:05,100
+هاي ونحط عليهم كمان مرة نينهيدرين بيتفاعلوا مع
+
+32
+00:02:05,100 --> 00:02:08,920
+بعض و بيعطيني حاجة اسمها Ruhemann's Complex Ruhemann's
+
+33
+00:02:08,920 --> 00:02:15,420
+Complex هذا لونه purple color إذا موجود الحنانة حمض
+
+34
+00:02:15,420 --> 00:02:18,100
+أميني، إذا كان يحتوي على Free Amino Alpha
+
+35
+00:02:18,100 --> 00:02:22,320
+Aminogram، بضيف عليه الـ Ninhydrin Reagent، المحصلة
+
+36
+00:02:22,320 --> 00:02:25,740
+كلها أنه في الآخر هيعطيني Ruhemann's Complex،
+
+37
+00:02:25,740 --> 00:02:29,280
+الـ Ninhydrin يشتغل على الـ Amino Acid، اللي يحتوي
+
+38
+00:02:29,280 --> 00:02:32,860
+على Free Alpha Aminogram، يعطيني حاجة بنسميها
+
+39
+00:02:32,860 --> 00:02:37,440
+Ruhemann's Complex، إيش لونها؟ لون purple إذا إذا
+
+40
+00:02:37,440 --> 00:02:41,140
+اتغيرت تغيرت الفحص وتغير اللون ل purple إذا هاي
+
+41
+00:02:41,140 --> 00:02:46,540
+المركب يحتوي على free alpha amino group إذا هو
+
+42
+00:02:46,540 --> 00:02:50,280
+positive يحتوي على free alpha amino group وهو
+
+43
+00:02:50,280 --> 00:02:50,900
+positive
+
+44
+00:02:54,400 --> 00:02:58,660
+هنا عندنا ال principle على شكل معادلة مرسوم فيها
+
+45
+00:02:58,660 --> 00:03:01,040
+structure إحنا مش مطلوب منا ال structure بس على
+
+46
+00:03:01,040 --> 00:03:04,620
+شكل معادلة حتى نلخص ال principle ويكون أثناء ال
+
+47
+00:03:04,620 --> 00:03:08,220
+principle أنه عندي حمض أميني Amino Acid بضيف عليها
+
+48
+00:03:08,220 --> 00:03:14,020
+Ninhydrin Reagent بيعطيني Hydrindantin و NH3 بأخذ
+
+49
+00:03:14,020 --> 00:03:16,920
+الـ Hydrindantin و الـ NH3 بضيف عليهم كمان
+
+50
+00:03:16,920 --> 00:03:20,840
+مرة Ninhydrin Reagent بيعطيني حاجة بنسميها Ruhemann's
+
+51
+00:03:20,840 --> 00:03:25,540
+Complex لونها purple هذا إذا كان المركب ال Amino
+
+52
+00:03:25,540 --> 00:03:29,000
+Acid يحتوي على free alpha amino group بيعطيني
+
+53
+00:03:29,000 --> 00:03:33,700
+Ruhemann's Complex لونها purple هذه المحصلة النهائية
+
+54
+00:03:41,910 --> 00:03:45,930
+البرولين يحتوي على amino group أو secondary amino
+
+55
+00:03:45,930 --> 00:03:50,030
+group لأن البرولين يتفاعل مع ال Ninhydrin ال amino
+
+56
+00:03:50,030 --> 00:03:53,450
+group اللي عنده بتتفاعل مع ال Ninhydrin بس لها طريقة
+
+57
+00:03:53,450 --> 00:03:57,370
+خاصة للتفاعل فبدل ما تعطيني لون purple بيعطيني لون
+
+58
+00:03:57,370 --> 00:04:02,090
+yellow لذلك كل الأحماض الأمينية تعطي قيمة positive
+
+59
+00:04:02,090 --> 00:04:06,570
+مع ال Ninhydrin لكن هل كلها بتعطي لون purple؟ لأ، كل
+
+60
+00:04:06,570 --> 00:04:10,230
+الأحماض الأمنية بتعطي لون purple ما عدا البرولين
+
+61
+00:04:10,230 --> 00:04:13,950
+بيعطيني لون yellow، تفاعل مع Ninhydrin بيعطيني لون
+
+62
+00:04:13,950 --> 00:04:18,770
+yellow Amino Acid Proline and Hydroxyproline react
+
+63
+00:04:18,770 --> 00:04:23,290
+with Ninhydrin but give yellow color من يعطيني
+
+64
+00:04:23,290 --> 00:04:26,610
+positive غير الـ Amino Acid besides Amino Acid من
+
+65
+00:04:26,610 --> 00:04:29,550
+هي يعطيني positive الأحماض الأمينية تعطيني positive مع
+
+66
+00:04:29,550 --> 00:04:33,070
+ال Ninhydrin كمان ال peptides البروتين ال
+
+67
+00:04:33,070 --> 00:04:35,950
+structure ال Primary Structure of البروتين نسميه
+
+68
+00:04:35,950 --> 00:04:36,530
+peptides
+
+69
+00:04:40,710 --> 00:04:44,290
+مرتبطة مع بعضها البعض في Peptide Bond الأحماض الأمينية
+
+70
+00:04:44,290 --> 00:04:47,230
+مرتبطة مع بعضها في Peptide Bond حكينا في جهة من
+
+71
+00:04:47,230 --> 00:04:51,030
+الجهات بيكون فيه Free Amino Group و جهة تانية فيه
+
+72
+00:04:51,030 --> 00:04:54,450
+Free Carboxyl Group إذا متوفر عندي Free Amino
+
+73
+00:04:54,450 --> 00:04:57,990
+Group، يعني إذا انطبق ال objective لـ Ninhydrin،
+
+74
+00:04:57,990 --> 00:05:01,720
+يعني هيعطيني positive الأحماض الأمنية هتعطيني positive
+
+75
+00:05:01,720 --> 00:05:05,540
+من الـ Ninhydrin الـ peptides اللي هي عبارة عن ترتيب
+
+76
+00:05:05,540 --> 00:05:09,680
+من أحماض أمينية مرتبطة مع بعضها البعض ب Peptide
+
+77
+00:05:09,680 --> 00:05:14,500
+Bond هتعطيني برضه positive الـ Peptone هو عبارة عن
+
+78
+00:05:14,500 --> 00:05:17,420
+الـ Protein مكسّر الحين أنا لما أكسر البروتين إيش
+
+79
+00:05:17,420 --> 00:05:21,560
+هينتج عندي؟ بينتج من البروتين Peptide Bond و Amino
+
+80
+00:05:21,560 --> 00:05:24,960
+Acid بدأ من نتج Amino Acid و ال Amino Acid بتحتوي
+
+81
+00:05:24,960 --> 00:05:27,420
+على Free Alpha Amino Group هيعطيني positive مع ال
+
+82
+00:05:27,420 --> 00:05:30,620
+Ninhydrin أو لا، هيعطيني positive إذا أن ال
+
+83
+00:05:30,620 --> 00:05:35,580
+Peptide و ال Peptone و البروتين و ال Amino Acid هم
+
+84
+00:05:35,580 --> 00:05:40,160
+الأربع بيعطوني positive مع ال Ninhydrin Ninhydrin
+
+85
+00:05:40,160 --> 00:05:44,480
+هيعطيني positive معاها طيب، احكي لي تحت إن الـ Ninhydrin
+
+86
+00:05:44,480 --> 00:05:48,480
+فيه إيثانول وهو “flammable” قابل للاشتعال الـ
+
+87
+00:05:48,480 --> 00:05:51,780
+“Reagent” هذا قابل للاشتعال “toxic substance” و
+
+88
+00:05:51,780 --> 00:05:55,560
+“irritant” مهيج فبدنا نحفظه بعيد عن اللهب عشان هو
+
+89
+00:05:55,560 --> 00:05:59,120
+قابل للاشتعال، بعيد عن العينين وعن الجلد لأنه مهيج
+
+90
+00:05:59,120 --> 00:06:05,100
+وبعيد عن الملابس وبدنا نتجنب استنشاق كمية كبيرة
+
+91
+00:06:05,100 --> 00:06:08,800
+منه لأنه مهيج فبدنا نتعامل معاه بحذر
+
+92
+00:06:12,040 --> 00:06:15,700
+هنا معادلة لتفاعل البرولين مع الـ Ninhydrin حكينا
+
+93
+00:06:15,700 --> 00:06:19,480
+له طريقة خاصة يقال له طريقة خاصة للتفاعل مع الـ Ninhydrin
+
+94
+00:06:19,480 --> 00:06:22,260
+بيعطينيش Ruhemann's Complex لونه purple وإنما
+
+95
+00:06:22,260 --> 00:06:26,280
+يعطيني Yellow Product ناتج لونه Yellow إذا تفاعل
+
+96
+00:06:26,280 --> 00:06:29,820
+البرولين مع ال Ninhydrin بيعطيني لون Yellow
+
+97
+00:06:33,100 --> 00:06:37,080
+هنا عندنا سؤال بيحكي لي ليش كل الأحماض الأمينية with
+
+98
+00:06:37,080 --> 00:06:40,880
+all amino acid مع Ninhydrin give purple but with
+
+99
+00:06:40,880 --> 00:06:43,120
+Proline مع Ninhydrin give yellow ليش بيعطيني yellow مع
+
+100
+00:06:43,120 --> 00:06:46,680
+البرولين ومع الباقي بيعطيني purple حكينا لأن
+
+101
+00:06:46,680 --> 00:06:51,520
+البرولين it have amino group but البرولين have
+
+102
+00:06:51,520 --> 00:06:56,350
+amino group not alpha amino group الـ Amino Group
+
+103
+00:06:56,350 --> 00:06:59,910
+هاي بتخلي بتعطيه Rigid Confirmation 
+
+104
+00:06:59,910 --> 00:07:03,310
+Confirmation Rigid Confirmation بتاعة المركب
+
+105
+00:07:03,310 --> 00:07:08,370
+صلابة فبيقلل ال flexibility لإنه بيخليه صعب التكسر
+
+106
+00:07:08,370 --> 00:07:11,770
+فبدل ما يتكسر لـ Hydrindantin و ال Amino Group
+
+107
+00:07:11,770 --> 00:07:16,130
+الـ NH3 ويعطيني Ruhemann's Complex لأ صعب إنه
+
+108
+00:07:16,130 --> 00:07:20,110
+يتكسر فله طريقة خاصة في التفاعل بيعطيني لون
+
+109
+00:07:20,110 --> 00:07:20,610
+yellow
+
+110
+00:07:23,370 --> 00:07:27,170
+في الـ Procedure خطوات العمل أنه نجيب Tube Glass
+
+111
+00:07:27,170 --> 00:07:30,030
+Tube بحط فيها 1 مل من الـ Solution اللي أنا
+
+112
+00:07:30,030 --> 00:07:33,390
+أعطاتكيه يعني أعطتك محلول مجهول بدك تفحصه هل
+
+113
+00:07:33,390 --> 00:07:37,090
+يحتوي على فيه Alpha Amino Group ولا لأ نحط 1 مل من
+
+114
+00:07:37,090 --> 00:07:40,510
+هذا الـ Solution مع 5 Drops من الـ Ninhydrin Agent
+
+115
+00:07:41,650 --> 00:07:45,270
+وبتحطيه في الـ Water Bath اللي بيحتوي على ماء مغلي
+
+116
+00:07:45,270 --> 00:07:50,410
+على Boiling Water Bath بحطه لمدة دقيقة بعد دقيقة
+
+117
+00:07:50,410 --> 00:07:53,970
+بطلع الـ Tube من الـ Water Bath إذا اتغير اللون ل
+
+118
+00:07:53,970 --> 00:07:58,130
+Purple Color إذا هذا المركب يحتوي على Free Alpha
+
+119
+00:07:58,130 --> 00:08:02,710
+Amino Group إذا ما تحولش اللون لـ Purple Color إذا
+
+120
+00:08:02,710 --> 00:08:06,290
+لا يحتوي على Free Alpha Amino Group طب إذا أعطاني
+
+121
+00:08:06,290 --> 00:08:11,920
+Yellow Color إذا بعرف أنه Proline Purple Color هيكون
+
+122
+00:08:11,920 --> 00:08:15,080
+أي مركب يحتوي على Free Alpha Amino Group ممكن يكون Amino
+
+123
+00:08:15,080 --> 00:08:19,380
+Acid، ممكن Peptide، ممكن Peptone، ممكن البروتين،
+
+124
+00:08:19,380 --> 00:08:24,160
+مهم يحتوي على Free Alpha Amino Group لكن if أعطاني
+
+125
+00:08:24,160 --> 00:08:27,060
+Yellow Color، بعرف إنه هذا Proline
+
+126
+00:08:29,890 --> 00:08:33,650
+الفحص الثاني هو فحص الـ Biuret Test، Biuret Test إيش
+
+127
+00:08:33,650 --> 00:08:36,290
+الـ Objective من الفحص؟ to detect the presence of
+
+128
+00:08:36,290 --> 00:08:40,290
+Peptide Bond، يدور على الـ Peptide Bond، إذا موجودة
+
+129
+00:08:40,290 --> 00:08:43,490
+الـ Peptide Bond، بيعطينا Positive، إذا مش موجودة،
+
+130
+00:08:43,490 --> 00:08:48,590
+هيعطينا Negative ومش هيتغير اللون إذا كيف وين
+
+131
+00:08:48,590 --> 00:08:51,410
+موجودة الـ Peptide Bond هتكون؟ هل موجودة في الحمض
+
+132
+00:08:51,410 --> 00:08:54,130
+الأميني؟ Single Amino Acid؟ هل الـ Structure فيه
+
+133
+00:08:54,130 --> 00:08:58,150
+Peptide Bond؟ الـ Structure العام للأمينو أسد؟ لأ،
+
+134
+00:08:58,150 --> 00:09:01,430
+إذا أن الأحماض الأمينية كلها هتعطيني Negative مع الـ Biuret
+
+135
+00:09:01,430 --> 00:09:06,050
+Test مين هيعطيني Positive؟ الـ Protein، لأن الـ
+
+136
+00:09:06,050 --> 00:09:10,010
+Protein هو عبارة عن ترتيب الأحماض الأمينية مرتبطة
+
+137
+00:09:10,010 --> 00:09:13,530
+مع بعضها البعض في Peptide Bond فالـ Protein هيعطيني
+
+138
+00:09:13,530 --> 00:09:20,910
+Positive، كمان عندما يتكسر البروتين لـ Peptide Bond،
+
+139
+00:09:20,910 --> 00:09:23,950
+فهي يعطيني Positive، لذلك البروتين والبيبتون
+
+140
+00:09:23,950 --> 00:09:28,030
+هيعطوني Positive قبل الـ Principle، المبدأ العام
+
+141
+00:09:28,030 --> 00:09:28,650
+للفحص
+
+142
+00:09:33,660 --> 00:09:37,800
+بضيف عليه Biuret Reagent إيش هو الـ Biuret Reagent؟
+
+143
+00:09:37,800 --> 00:09:43,340
+ما يبقى حاجته كبريتات نحاس with strong base كبريتات
+
+144
+00:09:43,340 --> 00:09:49,200
+نحاس CuSO4 في وجود Strong Base قاعدة قوية NaOH أو KOH
+
+145
+00:09:50,000 --> 00:09:53,660
+بيجي الـ Biuret Reagent الكبر اللي فيه بيروح على
+
+146
+00:09:53,660 --> 00:09:56,960
+الـ Peptide Bond هون بروتينات كلها بتتفاعل مع بعضها
+
+147
+00:09:56,960 --> 00:10:02,200
+ويعملهم كإنه مشبكة، بيعمل الكبر كـ Chelting Agent،
+
+148
+00:10:02,200 --> 00:10:06,500
+الكبر بيشتغل، بيشتغل كـ Chelting Agent، كعامل
+
+149
+00:10:06,500 --> 00:10:11,590
+متمخلب عامل متمخلب، يعني عامل زي مخلب، له أكتار
+
+150
+00:10:11,590 --> 00:10:14,630
+من إيه؟ في Middle من هنا ومن هنا ومن هنا، بيروح
+
+151
+00:10:14,630 --> 00:10:18,390
+كل الروابط البيبتدية الموجودة مع بعضها البعض، في
+
+152
+00:10:18,390 --> 00:10:23,570
+وجود القاعدة القوية، بيعمل Chelting Agent للكبر،
+
+153
+00:10:23,570 --> 00:10:26,670
+بيعمل Chelting Agent، عامل متمخلب
+
+154
+00:10:32,700 --> 00:10:37,240
+لونه Violet إذا تغير اللون لـ Violet إذا موجود عندي
+
+155
+00:10:37,240 --> 00:10:40,780
+الـ Peptide Bond إذا ما تغيرش إذا الـ Negative مش
+
+156
+00:10:40,780 --> 00:10:45,040
+موجودة بيحكي لي هذا اللون This Color is dependent on
+
+157
+00:10:45,040 --> 00:10:48,200
+the number of Peptide Bonds بيعتمد على عدد الـ
+
+158
+00:10:48,200 --> 00:10:51,400
+Peptide Bonds إذا الـ Peptide Bonds موجودة عندي اثنتين
+
+159
+00:10:51,400 --> 00:10:54,860
+بيكون Violet بس بشدة أخف من لما يكون عندي مثلًا
+
+160
+00:10:54,860 --> 00:10:59,460
+أربع روابط بيبتدية فكل ما زادت عدد روابط البيبتدية
+
+161
+00:10:59,460 --> 00:11:01,880
+كل ما زادت شدة اللون
+
+162
+00:11:06,510 --> 00:11:09,350
+في الـ Reagent الـ Biuret حكينا بيحتوي على كبريتات
+
+163
+00:11:09,350 --> 00:11:14,030
+نحاس with a Strong Base NaOH أو KOH ليش موجودة
+
+164
+00:11:14,030 --> 00:11:17,730
+الـ Strong Base الـ NaOH is there to raise the pH
+
+165
+00:11:17,730 --> 00:11:22,530
+عشان ترفع الـ pH لتوفير Alkaline Media لتوفير
+
+166
+00:11:22,530 --> 00:11:26,330
+Alkaline Media الـ pH اللي نحتاجها لتوفير Alkaline
+
+167
+00:11:26,330 --> 00:11:30,870
+Media طيب الميني المركب المهم في هذه في هذا
+
+168
+00:11:30,870 --> 00:11:31,930
+الـ Reagent الـ Copper
+
+169
+00:11:39,380 --> 00:11:43,180
+المركب المهم هو الـ Copper الـ Copper حكينا هو أهم
+
+170
+00:11:43,180 --> 00:11:47,440
+مركب موجود في الـ Reagent بيجي من الـ Copper Sulfate في
+
+171
+00:11:47,440 --> 00:11:51,740
+وجود Alkaline Solution إذا وجدت الـ Peptide Bonds و
+
+172
+00:11:51,740 --> 00:11:54,660
+الـ Peptide Bonds are present الـ Alkaline Solution
+
+173
+00:11:54,660 --> 00:11:58,220
+الـ Copper من الـ Copper Sulfate بيجي بيعطيني Complex
+
+174
+00:11:58,220 --> 00:12:03,600
+لونه Violet بيحكي لي إنه Two Peptide Bonds at least
+
+175
+00:12:03,600 --> 00:12:07,220
+are required for the formation of this Complex على
+
+176
+00:12:07,220 --> 00:12:11,660
+الأقل يكون موجود في المركب Two Peptide Bonds إذا
+
+177
+00:12:11,660 --> 00:12:15,660
+كان موجود رابطتين بيبتديتين أو أكثر بيشتغل الـ
+
+178
+00:12:15,660 --> 00:12:19,620
+Reagent وبيعطيني لون إذا رابطة بيبتدية واحدة بيقدرش
+
+179
+00:12:19,620 --> 00:12:23,000
+يشتغل ويعطيني Negative لأنه لو أجى الكبر على رابطة
+
+180
+00:12:23,000 --> 00:12:26,260
+بيبتدية واحدة الكي ليت يعمل عامل متمخلب وبأنه بد
+
+201
+00:13:57,990 --> 00:14:01,810
+إذا positive هيتغير لـviolet، فإذا ما تغيرش اللون
+
+202
+00:14:01,810 --> 00:14:05,230
+وضلوا أزرق، معناها البروتين مش موجود يعني ممكن
+
+203
+00:14:05,230 --> 00:14:08,370
+يكون موجود عندي حمض أميني، المهم الـpeptide bond مش
+
+204
+00:14:08,370 --> 00:14:13,110
+موجودة، فاللون ما تغيرش، ماصارش تفاعل إذا اللون
+
+205
+00:14:13,110 --> 00:14:17,470
+اتغير، بيحكيلي إلى violet من blue لـviolet لدرجة ال
+
+206
+00:14:17,470 --> 00:14:21,710
+purple، الغامق، إذا البروتين موجود طب إذا اتغير لل
+
+207
+00:14:21,710 --> 00:14:25,930
+pink لدرجة أفتح من الـviolet، اللون pink، إذا اللي
+
+208
+00:14:25,930 --> 00:14:30,900
+موجود بـpeptide bond، ولكن عددها قليل، أقليعني ممكن
+
+209
+00:14:30,900 --> 00:14:35,280
+يكون موجود وعندي بيبتون اللي هو عند بروتين مكسّر،
+
+210
+00:14:35,280 --> 00:14:39,460
+عدد الـpeptide bond اللي فيه قليل، فدرجة
+
+211
+00:14:39,460 --> 00:14:40,980
+اللون بتكون أقل.
+
+212
+00:14:43,720 --> 00:14:48,300
+البحث اللي بعد هيك هو الـxanthoproteic test الـ
+
+213
+00:14:48,300 --> 00:14:51,140
+xanthoproteic test إيش الـobjective منه؟ to
+
+214
+00:14:51,140 --> 00:14:55,720
+differentiate between aromatic and amino acid، لأ،
+
+215
+00:14:55,720 --> 00:14:59,320
+بس ده الـamino acid اللي بيحتوي على benzene ring
+
+216
+00:14:59,320 --> 00:15:02,660
+على حلقة بنزين، إذا فاكرين الصورة دي اللي حكينا
+
+217
+00:15:02,660 --> 00:15:05,880
+إنه في بعض الأحماض الأمينية تسميها aromatic amino
+
+218
+00:15:05,880 --> 00:15:10,250
+acid تحتوي على حلقة بنزين، بس منضيف هنا إنها تكون
+
+219
+00:15:10,250 --> 00:15:14,350
+Activated Aromatic Amino Acid تكون حلقة البنزين
+
+220
+00:15:14,350 --> 00:15:18,270
+مشطعة، أنه في حمض أميني، حلقة البنزين اللي عنده
+
+221
+00:15:18,270 --> 00:15:22,790
+بتكون Inactive، إذا يحتوي على Aromatic Activated
+
+222
+00:15:22,790 --> 00:15:26,610
+Aromatic Benzene Ring أو Aromatic Amino Acid بيكون
+
+223
+00:15:26,610 --> 00:15:29,570
+... بيعطيني لون Yellow، بيكون Positive، إذا لا
+
+224
+00:15:29,570 --> 00:15:33,690
+يحتوي، هيكون Negative، فكيف الـprinciple مبدأ عمل
+
+225
+00:15:33,690 --> 00:15:38,950
+الفحص أنه بيجي عنده amino acid with aromatic ring
+
+226
+00:15:38,950 --> 00:15:42,930
+حمض أميني بيحتوي على حلقة بنزين بحط عليه
+
+227
+00:15:42,930 --> 00:15:47,190
+concentrated nitric acid concentrated nitric acid
+
+228
+00:15:47,190 --> 00:15:54,110
+HNO3 في وجود قاعدة قوية زي الـNaOH في وجود NaOH
+
+229
+00:15:54,110 --> 00:15:58,050
+ليش هييجي يعمل الـnitric acid لحمض الأميني اللي
+
+230
+00:15:58,050 --> 00:16:02,910
+يحتوي على بنزين ring؟ يعمل نيترياشيل of بنزين رينغ. 
diff --git a/PL9fwy3NUQKwZ1Q7zOg1ObgufPMjRI3WY1/iXxjfqDroRk_raw.json b/PL9fwy3NUQKwZ1Q7zOg1ObgufPMjRI3WY1/iXxjfqDroRk_raw.json
new file mode 100644
index 0000000000000000000000000000000000000000..d8c1356201c4ee7b8cb5d127a822a96d6dfe71fa
--- /dev/null
+++ b/PL9fwy3NUQKwZ1Q7zOg1ObgufPMjRI3WY1/iXxjfqDroRk_raw.json
@@ -0,0 +1 @@
+{"segments": [{"id": 1, "seek": 2710, "start": 5.48, "end": 27.1, "text": "أول فحص هو الـ Nihidrine الـ Nihidrine إيش ال objective لو فحصتش الهدف من الفحص to detect the presence of a free alpha amino group بيدور على ال free alpha amino group إذا موجود free alpha amino group هيتغير اللون، بيعطيني اللون purple في حالات اللي موجودة في free alpha amino group", "tokens": [10721, 12610, 6156, 5016, 9381, 31439, 2423, 39184, 426, 4247, 327, 15140, 2423, 39184, 426, 4247, 327, 15140, 11933, 1829, 8592, 2423, 10024, 45164, 6156, 5016, 9381, 2655, 8592, 2423, 3224, 3215, 5172, 9154, 27188, 5016, 9381, 281, 5531, 264, 6814, 295, 257, 1737, 8961, 24674, 1594, 4724, 25708, 13063, 15844, 2423, 1737, 8961, 24674, 1594, 11933, 15730, 3714, 29245, 23328, 1737, 8961, 24674, 1594, 39896, 2655, 17082, 13546, 13672, 11536, 12399, 4724, 40228, 9566, 1829, 22653, 13672, 11536, 9656, 8978, 11331, 6027, 9307, 13672, 1829, 3714, 29245, 23328, 3660, 8978, 1737, 8961, 24674, 1594], "avg_logprob": -0.22688801943634948, "compression_ratio": 1.7757009345794392, "no_speech_prob": 3.427267074584961e-05, "words": [{"start": 5.48, "end": 5.84, "word": "أول", "probability": 0.916748046875}, {"start": 5.84, "end": 6.28, "word": " فحص", "probability": 0.9521484375}, {"start": 6.28, "end": 6.52, "word": " هو", "probability": 0.89599609375}, {"start": 6.52, "end": 6.66, "word": " الـ", "probability": 0.84912109375}, {"start": 6.66, "end": 7.32, "word": " Nihidrine", "probability": 0.3489990234375}, {"start": 7.32, "end": 8.2, "word": " الـ", "probability": 0.68994140625}, {"start": 8.2, "end": 8.84, "word": " Nihidrine", "probability": 0.872314453125}, {"start": 8.84, "end": 9.18, "word": " إيش", "probability": 0.6677042643229166}, {"start": 9.18, "end": 9.28, "word": " ال", "probability": 0.97607421875}, {"start": 9.28, "end": 9.74, "word": " objective", "probability": 0.490478515625}, {"start": 9.74, "end": 9.98, "word": " لو", "probability": 0.8671875}, {"start": 9.98, "end": 10.38, "word": " فحصتش", "probability": 0.741748046875}, {"start": 10.38, "end": 10.7, "word": " الهدف", "probability": 0.9306640625}, {"start": 10.7, "end": 10.88, "word": " من", "probability": 0.95556640625}, {"start": 10.88, "end": 11.38, "word": " الفحص", "probability": 0.9757486979166666}, {"start": 11.38, "end": 11.82, "word": " to", "probability": 0.473876953125}, {"start": 11.82, "end": 12.26, "word": " detect", "probability": 0.9873046875}, {"start": 12.26, "end": 12.7, "word": " the", "probability": 0.92138671875}, {"start": 12.7, "end": 13.18, "word": " presence", "probability": 0.95068359375}, {"start": 13.18, "end": 13.38, "word": " of", "probability": 0.9853515625}, {"start": 13.38, "end": 13.52, "word": " a", "probability": 0.345458984375}, {"start": 13.52, "end": 13.74, "word": " free", "probability": 0.84033203125}, {"start": 13.74, "end": 14.2, "word": " alpha", "probability": 0.69677734375}, {"start": 14.2, "end": 14.58, "word": " amino", "probability": 0.8505859375}, 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41184, 12399, 2423, 39184, 45, 545, 6655, 12629, 7251, 8592, 2655, 17082, 1211, 15844, 2423, 39184, 32, 2367, 78, 5097, 327, 12399, 13672, 1829, 7251, 33753, 45865, 15844, 11551, 20588, 2012, 259, 12820, 12399, 37495, 9566, 9957, 1829, 11331, 26108, 3660, 4724, 1863, 38251, 1829, 11296, 20252, 41184, 12399, 11933, 1829, 8592, 5296, 11536, 11296, 22807, 5296, 11536, 4724, 13546, 3555, 11536], "avg_logprob": -0.2527067046465836, "compression_ratio": 1.6901960784313725, "no_speech_prob": 5.662441253662109e-06, "words": [{"start": 133.26, "end": 133.58, "word": "إذا", "probability": 0.75390625}, {"start": 133.58, "end": 134.58, "word": " موجود", "probability": 0.7913411458333334}, {"start": 134.58, "end": 135.14, "word": " الحنانة", "probability": 0.68670654296875}, {"start": 135.14, "end": 135.42, "word": " حمض", "probability": 0.7416178385416666}, {"start": 135.42, "end": 136.06, "word": " أميني،", "probability": 0.740966796875}, {"start": 136.06, "end": 136.24, "word": " إذا", "probability": 0.909423828125}, {"start": 136.24, "end": 136.48, "word": " كان", "probability": 0.98486328125}, {"start": 136.48, "end": 136.86, "word": " يحتوي", "probability": 0.97509765625}, {"start": 136.86, "end": 137.0, "word": " على", "probability": 0.9140625}, {"start": 137.0, "end": 137.32, "word": " Free", "probability": 0.265380859375}, {"start": 137.32, "end": 137.86, "word": " Amino", "probability": 0.76025390625}, {"start": 137.86, "end": 138.1, "word": " Alpha", "probability": 0.5048828125}, {"start": 138.1, "end": 139.24, "word": " Aminogram،", "probability": 0.654541015625}, {"start": 139.24, "end": 139.66, "word": " بضيف", "probability": 0.85009765625}, {"start": 139.66, "end": 139.98, "word": " عليه", "probability": 0.87353515625}, {"start": 139.98, "end": 140.66, "word": " الـNahydrin", "probability": 0.6421915690104166}, {"start": 140.66, "end": 141.64, "word": " Reagent،", "probability": 0.75732421875}, {"start": 141.64, "end": 142.32, "word": " المحصلة", "probability": 0.8720703125}, {"start": 142.32, "end": 142.74, "word": " كلها", "probability": 0.9951171875}, {"start": 142.74, "end": 142.96, "word": " أنه", "probability": 0.7177734375}, {"start": 142.96, "end": 143.04, "word": " في", "probability": 0.984375}, {"start": 143.04, "end": 143.42, "word": " الآخر", "probability": 0.9825846354166666}, {"start": 143.42, "end": 144.2, "word": " هيعطيني", "probability": 0.920703125}, {"start": 144.2, "end": 145.0, "word": " Romans", "probability": 0.30322265625}, {"start": 145.0, "end": 145.74, "word": " Complex،", "probability": 0.6697998046875}, {"start": 145.74, "end": 146.58, "word": " الـNahydrin", "probability": 0.9302571614583334}, {"start": 146.58, "end": 147.36, "word": " يشتغل", "probability": 0.82958984375}, {"start": 147.36, "end": 147.62, "word": " على", "probability": 0.95654296875}, {"start": 147.62, "end": 148.22, "word": " الـAmino", "probability": 0.8173828125}, {"start": 148.22, "end": 148.72, "word": " Acid،", "probability": 0.7493489583333334}, {"start": 148.72, "end": 148.82, "word": " اللي", "probability": 0.952880859375}, {"start": 148.82, "end": 149.28, "word": " يحتوي", "probability": 0.974609375}, {"start": 149.28, "end": 149.44, "word": " على", "probability": 0.9384765625}, {"start": 149.44, "end": 149.76, "word": " Free", "probability": 0.84814453125}, {"start": 149.76, "end": 150.18, "word": " Alpha", "probability": 0.81591796875}, {"start": 150.18, "end": 151.44, "word": " Aminogram،", "probability": 0.8607177734375}, {"start": 151.44, "end": 152.0, "word": " يعطيني", "probability": 0.941650390625}, {"start": 152.0, "end": 152.26, "word": " حاجة", "probability": 0.9964192708333334}, {"start": 152.26, "end": 152.86, "word": " بنسميها", "probability": 0.82939453125}, {"start": 152.86, "end": 153.42, "word": " Romans", "probability": 0.8740234375}, {"start": 153.42, "end": 154.46, "word": " Complex،", "probability": 0.877197265625}, {"start": 154.46, "end": 154.72, "word": " إيش", "probability": 0.8636067708333334}, {"start": 154.72, "end": 155.48, "word": " لونها؟", "probability": 0.73956298828125}, {"start": 155.48, "end": 155.88, "word": " لون", "probability": 0.98486328125}, {"start": 155.88, "end": 156.52, "word": " بيربون", "probability": 0.6959228515625}], "temperature": 1.0}, {"id": 8, "seek": 17090, "start": 156.78, "end": 170.9, "text": "إذا إذا اتغيرت تغييرت الفحص وتغير اللون ل purple إذا هاي المركب يحتوي على free alpha amino group إذا هو positive يحتوي على free alpha amino group وهو positive", "tokens": [28814, 15730, 11933, 15730, 1975, 2655, 17082, 13546, 2655, 6055, 17082, 1829, 13546, 2655, 27188, 5016, 9381, 34683, 17082, 13546, 13672, 11536, 5296, 9656, 11933, 15730, 8032, 47302, 9673, 31747, 3555, 7251, 33753, 45865, 15844, 1737, 8961, 24674, 1594, 11933, 15730, 31439, 3353, 7251, 33753, 45865, 15844, 1737, 8961, 24674, 1594, 37037, 2407, 3353], "avg_logprob": -0.32045455195687034, "compression_ratio": 1.7014925373134329, "no_speech_prob": 1.4901161193847656e-06, "words": [{"start": 156.78, "end": 157.2, "word": "إذا", "probability": 0.779541015625}, {"start": 157.2, "end": 157.44, "word": " إذا", "probability": 0.60400390625}, {"start": 157.44, "end": 157.94, "word": " اتغيرت", "probability": 0.79990234375}, {"start": 157.94, "end": 158.26, "word": " تغييرت", "probability": 0.60205078125}, {"start": 158.26, "end": 158.74, "word": " الفحص", "probability": 0.92724609375}, {"start": 158.74, "end": 159.26, "word": " وتغير", "probability": 0.8203125}, {"start": 159.26, "end": 159.52, "word": " اللون", "probability": 0.71337890625}, {"start": 159.52, "end": 159.74, "word": " ل", "probability": 0.92919921875}, {"start": 159.74, "end": 160.16, "word": " purple", "probability": 0.09039306640625}, {"start": 160.16, "end": 160.76, "word": " إذا", "probability": 0.781005859375}, {"start": 160.76, "end": 161.14, "word": " هاي", "probability": 0.5443115234375}, {"start": 161.14, "end": 161.76, "word": " المركب", "probability": 0.8611653645833334}, {"start": 161.76, "end": 162.38, "word": " يحتوي", "probability": 0.9817708333333334}, {"start": 162.38, "end": 162.64, "word": " على", "probability": 0.9375}, {"start": 162.64, "end": 163.08, "word": " free", "probability": 0.720703125}, {"start": 163.08, "end": 164.0, "word": " alpha", "probability": 0.66845703125}, {"start": 164.0, "end": 164.8, "word": " amino", "probability": 0.8994140625}, {"start": 164.8, "end": 165.78, "word": " group", "probability": 0.97705078125}, {"start": 165.78, "end": 166.2, "word": " إذا", "probability": 0.5380859375}, {"start": 166.2, "end": 166.54, "word": " هو", "probability": 0.9462890625}, {"start": 166.54, "end": 167.2, "word": " positive", "probability": 0.90283203125}, {"start": 167.2, "end": 167.9, "word": " يحتوي", "probability": 0.8736979166666666}, {"start": 167.9, "end": 168.42, "word": " على", "probability": 0.94140625}, {"start": 168.42, "end": 168.72, "word": " free", "probability": 0.93408203125}, {"start": 168.72, "end": 169.04, "word": " alpha", "probability": 0.8583984375}, {"start": 169.04, "end": 169.38, "word": " amino", "probability": 0.966796875}, {"start": 169.38, "end": 169.8, "word": " group", "probability": 0.98193359375}, {"start": 169.8, "end": 170.28, "word": " وهو", "probability": 0.761474609375}, {"start": 170.28, "end": 170.9, "word": " positive", "probability": 0.912109375}], "temperature": 1.0}, {"id": 9, "seek": 19160, "start": 174.4, "end": 191.6, "text": "هنا عندنا ال principle على شكل معادلة مرسوم فيها structure احنا مش مطلوب منها ال structure بس على شكل معادلة حتى نلخص ال principle و يكون أثناء ال principle أنه عندي حمض أميني أمينو أسد بضيف عليها نهيدرين راجع بيعطيني هيدرين دنتين", "tokens": [3224, 8315, 43242, 8315, 2423, 8665, 15844, 13412, 28820, 20449, 18513, 37977, 3714, 2288, 3794, 20498, 8978, 11296, 3877, 1975, 5016, 8315, 37893, 3714, 9566, 1211, 37746, 9154, 11296, 2423, 3877, 4724, 3794, 15844, 13412, 28820, 20449, 18513, 37977, 11331, 49975, 8717, 1211, 9778, 9381, 2423, 8665, 4032, 7251, 30544, 5551, 12984, 1863, 16606, 2423, 8665, 14739, 3224, 18871, 16254, 11331, 2304, 11242, 5551, 2304, 9957, 1829, 5551, 2304, 9957, 2407, 5551, 3794, 3215, 4724, 11242, 33911, 25894, 11296, 8717, 3224, 25708, 2288, 9957, 12602, 26108, 3615, 4724, 40228, 9566, 1829, 22653, 8032, 25708, 2288, 9957, 11778, 29399, 9957], "avg_logprob": -0.20109375, "compression_ratio": 1.87, "no_speech_prob": 1.0073184967041016e-05, "words": [{"start": 174.4, "end": 174.74, "word": "هنا", "probability": 0.925048828125}, {"start": 174.74, "end": 175.06, "word": " عندنا", "probability": 0.747802734375}, {"start": 175.06, "end": 175.18, "word": " ال", "probability": 0.89697265625}, {"start": 175.18, "end": 175.62, "word": " principle", "probability": 0.372314453125}, {"start": 175.62, "end": 175.98, "word": " على", "probability": 0.86572265625}, {"start": 175.98, "end": 176.46, "word": " شكل", "probability": 0.9833984375}, {"start": 176.46, "end": 177.22, "word": " معادلة", "probability": 0.99365234375}, {"start": 177.22, "end": 178.46, "word": " مرسوم", "probability": 0.7369384765625}, {"start": 178.46, "end": 178.66, "word": " فيها", "probability": 0.9326171875}, {"start": 178.66, "end": 179.12, "word": " structure", "probability": 0.79541015625}, {"start": 179.12, "end": 179.38, "word": " احنا", "probability": 0.78955078125}, {"start": 179.38, "end": 179.54, "word": " مش", "probability": 0.99072265625}, {"start": 179.54, "end": 179.86, "word": " مطلوب", "probability": 0.8541259765625}, {"start": 179.86, "end": 180.06, "word": " منها", "probability": 0.852783203125}, {"start": 180.06, "end": 180.14, "word": " ال", "probability": 0.8857421875}, {"start": 180.14, "end": 180.58, "word": " structure", "probability": 0.7646484375}, {"start": 180.58, "end": 180.9, "word": " بس", "probability": 0.943115234375}, {"start": 180.9, "end": 181.04, "word": " على", "probability": 0.9150390625}, {"start": 181.04, "end": 181.32, "word": " شكل", "probability": 0.98095703125}, {"start": 181.32, "end": 181.78, "word": " معادلة", "probability": 0.9736328125}, {"start": 181.78, "end": 182.0, "word": " حتى", "probability": 0.869140625}, {"start": 182.0, "end": 182.46, "word": " نلخص", "probability": 0.9664306640625}, {"start": 182.46, "end": 182.62, "word": " ال", "probability": 0.98876953125}, {"start": 182.62, "end": 183.08, "word": " principle", "probability": 0.9365234375}, {"start": 183.08, "end": 183.3, "word": " و", "probability": 0.9326171875}, {"start": 183.3, "end": 183.52, "word": " يكون", "probability": 0.848876953125}, {"start": 183.52, "end": 184.08, "word": " أثناء", "probability": 0.474212646484375}, {"start": 184.08, "end": 184.62, "word": " ال", "probability": 0.9912109375}, {"start": 184.62, "end": 185.06, "word": " principle", "probability": 0.93017578125}, {"start": 185.06, "end": 185.46, "word": " أنه", "probability": 0.6768798828125}, {"start": 185.46, "end": 185.72, "word": " عندي", "probability": 0.89599609375}, {"start": 185.72, "end": 186.04, "word": " حمض", "probability": 0.9768880208333334}, {"start": 186.04, "end": 186.42, "word": " أميني", "probability": 0.867431640625}, {"start": 186.42, "end": 186.8, "word": " أمينو", "probability": 0.8250732421875}, {"start": 186.8, "end": 187.16, "word": " أسد", "probability": 0.8307291666666666}, {"start": 187.16, "end": 187.82, "word": " بضيف", "probability": 0.8473307291666666}, {"start": 187.82, "end": 188.22, "word": " عليها", "probability": 0.689453125}, {"start": 188.22, "end": 188.92, "word": " نهيدرين", "probability": 0.90673828125}, {"start": 188.92, "end": 189.5, "word": " راجع", "probability": 0.84033203125}, {"start": 189.5, "end": 190.54, "word": " بيعطيني", "probability": 0.8041015625}, {"start": 190.54, "end": 191.08, "word": " هيدرين", "probability": 0.7593994140625}, {"start": 191.08, "end": 191.6, "word": " دنتين", "probability": 0.7594401041666666}], "temperature": 1.0}, {"id": 10, "seek": 21370, "start": 192.12, "end": 213.7, "text": "و Anacetrate باخد الهيدرين دنتين و ال Anacetrate باطيف عليهم كمان مرانين هيدرين رياجان بيعطيني حاجة بنسميها romance complex لغنها purple هذا إذا كان المركب ال amino acid يحتوي على free alpha amino group بيعطيني romance complex لغنه purple هذه المحصلة النهائية", "tokens": [2407, 1107, 326, 302, 4404, 4724, 47283, 3215, 2423, 3224, 25708, 2288, 9957, 11778, 29399, 9957, 4032, 2423, 1107, 326, 302, 4404, 4724, 41193, 33911, 25894, 16095, 9122, 2304, 7649, 3714, 2288, 7649, 9957, 8032, 25708, 2288, 9957, 12602, 1829, 26108, 7649, 4724, 40228, 9566, 1829, 22653, 11331, 26108, 3660, 44945, 38251, 1829, 11296, 19064, 3997, 5296, 17082, 1863, 11296, 9656, 23758, 11933, 15730, 25961, 9673, 31747, 3555, 2423, 24674, 8258, 7251, 33753, 45865, 15844, 1737, 8961, 24674, 1594, 4724, 40228, 9566, 1829, 22653, 19064, 3997, 5296, 17082, 1863, 3224, 9656, 29538, 9673, 5016, 9381, 37977, 28239, 3224, 16373, 10632], "avg_logprob": -0.3013613790568739, "compression_ratio": 1.7522522522522523, "no_speech_prob": 0.0002474784851074219, "words": [{"start": 192.12, "end": 192.42, "word": "و", "probability": 0.77197265625}, {"start": 192.42, "end": 193.24, "word": " Anacetrate", "probability": 0.318634033203125}, {"start": 193.24, "end": 194.02, "word": " باخد", "probability": 0.9488932291666666}, {"start": 194.02, "end": 194.54, "word": " الهيدرين", "probability": 0.8024169921875}, {"start": 194.54, "end": 195.0, "word": " دنتين", "probability": 0.7137858072916666}, {"start": 195.0, "end": 195.1, "word": " و", "probability": 0.32861328125}, {"start": 195.1, "end": 195.2, "word": " ال", "probability": 0.73974609375}, {"start": 195.2, "end": 195.94, "word": " Anacetrate", "probability": 0.7908935546875}, {"start": 195.94, "end": 196.32, "word": " باطيف", "probability": 0.7537434895833334}, {"start": 196.32, "end": 196.6, "word": " عليهم", "probability": 0.953125}, {"start": 196.6, "end": 196.92, "word": " كمان", "probability": 0.9122721354166666}, {"start": 196.92, "end": 197.4, "word": " مرانين", "probability": 0.85009765625}, {"start": 197.4, "end": 197.84, "word": " هيدرين", "probability": 0.784820556640625}, {"start": 197.84, "end": 198.4, "word": " رياجان", "probability": 0.787109375}, {"start": 198.4, "end": 199.54, "word": " بيعطيني", "probability": 0.8380859375}, {"start": 199.54, "end": 199.82, "word": " حاجة", "probability": 0.98974609375}, {"start": 199.82, "end": 200.34, "word": " بنسميها", "probability": 0.833740234375}, {"start": 200.34, "end": 200.84, "word": " romance", "probability": 0.267822265625}, {"start": 200.84, "end": 201.54, "word": " complex", "probability": 0.9072265625}, {"start": 201.54, "end": 202.42, "word": " لغنها", "probability": 0.77349853515625}, {"start": 202.42, "end": 202.82, "word": " purple", "probability": 0.79541015625}, {"start": 202.82, "end": 203.6, "word": " هذا", "probability": 0.638671875}, {"start": 203.6, "end": 203.84, "word": " إذا", "probability": 0.912109375}, {"start": 203.84, "end": 204.14, "word": " كان", "probability": 0.9951171875}, {"start": 204.14, "end": 204.7, "word": " المركب", "probability": 0.8741861979166666}, {"start": 204.7, "end": 204.88, "word": " ال", "probability": 0.70947265625}, {"start": 204.88, "end": 205.54, "word": " amino", "probability": 0.265380859375}, {"start": 205.54, "end": 205.82, "word": " acid", "probability": 0.82763671875}, {"start": 205.82, "end": 206.18, "word": " يحتوي", "probability": 0.9641927083333334}, {"start": 206.18, "end": 206.34, "word": " على", "probability": 0.9150390625}, {"start": 206.34, "end": 206.66, "word": " free", "probability": 0.6328125}, {"start": 206.66, "end": 207.18, "word": " alpha", "probability": 0.6826171875}, {"start": 207.18, "end": 207.6, "word": " amino", "probability": 0.9423828125}, {"start": 207.6, "end": 208.06, "word": " group", "probability": 0.96533203125}, {"start": 208.06, "end": 209.0, "word": " بيعطيني", "probability": 0.7623046875}, {"start": 209.0, "end": 209.52, "word": " romance", "probability": 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0.9879557291666666}, {"start": 365.1, "end": 365.44, "word": " منه", "probability": 0.99072265625}, {"start": 365.44, "end": 365.9, "word": " لأنه", "probability": 0.9436848958333334}, {"start": 365.9, "end": 366.58, "word": " مهيج", "probability": 0.9967041015625}, {"start": 366.58, "end": 367.48, "word": " فبدنا", "probability": 0.9182942708333334}, {"start": 367.48, "end": 368.02, "word": " نتعامل", "probability": 0.996875}, {"start": 368.02, "end": 368.34, "word": " معاه", "probability": 0.9931640625}, {"start": 368.34, "end": 368.8, "word": " بحذر", "probability": 0.909912109375}], "temperature": 1.0}, {"id": 18, "seek": 38982, "start": 372.04, "end": 389.82, "text": "هنا معادلة لاتفاعل البرولين مع ال نينهيدرين حكينا إله خاص يقاله طريقة خاصة لاتفاعل مع ال نينهيدرين بيعطينيش romance complex اللونه purple وإنما يعطيني yellow product ناتج لونه yellow إذا تفاعل البرولين مع ال نينهيدرين بيعطيني لون yellow", "tokens": [3224, 8315, 20449, 18513, 37977, 5296, 9307, 5172, 995, 30241, 2423, 26890, 12610, 9957, 20449, 2423, 8717, 9957, 3224, 25708, 2288, 9957, 11331, 4117, 1829, 8315, 11933, 43761, 16490, 33546, 7251, 4587, 6027, 3224, 23032, 16572, 28671, 16490, 33546, 3660, 5296, 9307, 5172, 995, 30241, 20449, 2423, 8717, 9957, 3224, 25708, 2288, 9957, 4724, 40228, 9566, 1829, 22653, 8592, 19064, 3997, 13672, 11536, 3224, 9656, 4032, 28814, 1863, 15042, 37495, 9566, 9957, 1829, 5566, 1674, 8717, 9307, 7435, 5296, 11536, 3224, 5566, 11933, 15730, 6055, 5172, 995, 30241, 2423, 26890, 12610, 9957, 20449, 2423, 8717, 9957, 3224, 25708, 2288, 9957, 4724, 40228, 9566, 9957, 1829, 5296, 11536, 5566], "avg_logprob": -0.20255160058310273, "compression_ratio": 2.0526315789473686, "no_speech_prob": 2.86102294921875e-06, "words": [{"start": 372.04, "end": 372.44, "word": "هنا", "probability": 0.960693359375}, {"start": 372.44, "end": 373.1, "word": " معادلة", "probability": 0.9830729166666666}, {"start": 373.1, "end": 373.68, "word": " لاتفاعل", "probability": 0.79365234375}, {"start": 373.68, "end": 374.2, "word": " البرولين", "probability": 0.75762939453125}, {"start": 374.2, "end": 374.38, "word": " مع", "probability": 0.98486328125}, {"start": 374.38, "end": 374.5, "word": " ال", "probability": 0.96435546875}, {"start": 374.5, "end": 375.06, "word": " نينهيدرين", "probability": 0.6305948893229166}, {"start": 375.06, "end": 375.7, "word": " حكينا", "probability": 0.9571533203125}, {"start": 375.7, "end": 375.92, "word": " إله", "probability": 0.4202880859375}, {"start": 375.92, "end": 376.4, "word": " خاص", "probability": 0.9765625}, {"start": 376.4, "end": 377.12, "word": " يقاله", "probability": 0.66986083984375}, {"start": 377.12, "end": 377.82, "word": " طريقة", "probability": 0.9622395833333334}, {"start": 377.82, "end": 378.18, "word": " خاصة", "probability": 0.9640299479166666}, {"start": 378.18, "end": 378.58, "word": " لاتفاعل", "probability": 0.85927734375}, {"start": 378.58, "end": 378.74, "word": " مع", "probability": 0.98828125}, {"start": 378.74, "end": 378.84, "word": " ال", "probability": 0.95703125}, {"start": 378.84, "end": 379.48, "word": " نينهيدرين", "probability": 0.9315592447916666}, {"start": 379.48, "end": 380.08, "word": " بيعطينيش", "probability": 0.7124837239583334}, {"start": 380.08, "end": 380.44, "word": " romance", "probability": 0.429931640625}, {"start": 380.44, "end": 381.0, "word": " complex", "probability": 0.916015625}, {"start": 381.0, "end": 381.44, "word": " اللونه", "probability": 0.75146484375}, {"start": 381.44, "end": 381.72, "word": " purple", "probability": 0.95458984375}, {"start": 381.72, "end": 382.26, "word": " وإنما", "probability": 0.892822265625}, {"start": 382.26, "end": 382.84, "word": " يعطيني", "probability": 0.880859375}, {"start": 382.84, "end": 383.08, "word": " yellow", "probability": 0.7607421875}, {"start": 383.08, "end": 383.62, "word": " product", "probability": 0.97412109375}, {"start": 383.62, "end": 384.52, "word": " ناتج", "probability": 0.9339192708333334}, {"start": 384.52, "end": 385.0, "word": " لونه", "probability": 0.9524739583333334}, {"start": 385.0, "end": 385.36, "word": " yellow", "probability": 0.8251953125}, {"start": 385.36, "end": 385.76, "word": " إذا", "probability": 0.9130859375}, {"start": 385.76, "end": 386.28, "word": " تفاعل", "probability": 0.873046875}, {"start": 386.28, "end": 386.78, "word": " البرولين", "probability": 0.8934326171875}, {"start": 386.78, "end": 386.94, "word": " مع", "probability": 0.99609375}, {"start": 386.94, "end": 387.08, "word": " ال", "probability": 0.97119140625}, {"start": 387.08, "end": 387.72, "word": " نينهيدرين", "probability": 0.95166015625}, {"start": 387.72, "end": 388.58, "word": " بيعطيني", "probability": 0.841796875}, {"start": 388.58, "end": 389.3, "word": " لون", "probability": 0.95263671875}, {"start": 389.3, "end": 389.82, "word": " yellow", "probability": 0.7802734375}], "temperature": 1.0}, {"id": 19, "seek": 41470, "start": 393.1, "end": 414.7, "text": "هنا عنا سؤال بيحكي لي ليش كل الأحماض الأمينية with all amino acid منها يدرين give purple but with proline يدرين give yellow ليش ميعتنين yellow مع البرولين و مع الباقي بيعطيني purple حكينا لأن البرولين it have amino group but البرولين have amino group not alpha amino group", "tokens": [3224, 8315, 6225, 8315, 8608, 33604, 6027, 4724, 1829, 5016, 4117, 1829, 32239, 32239, 8592, 28242, 16247, 35571, 46958, 16247, 2304, 9957, 10632, 365, 439, 24674, 8258, 9154, 11296, 7251, 3215, 2288, 9957, 976, 9656, 457, 365, 447, 1889, 7251, 3215, 2288, 9957, 976, 5566, 32239, 8592, 3714, 40228, 2655, 1863, 9957, 5566, 20449, 2423, 26890, 12610, 9957, 4032, 20449, 29739, 995, 38436, 4724, 40228, 9566, 1829, 22653, 9656, 11331, 4117, 1829, 8315, 5296, 33456, 2423, 26890, 12610, 9957, 309, 362, 24674, 1594, 457, 2423, 26890, 12610, 9957, 362, 24674, 1594, 406, 8961, 24674, 1594], "avg_logprob": -0.2875976621483763, "compression_ratio": 1.8592233009708738, "no_speech_prob": 1.430511474609375e-06, "words": [{"start": 393.1, 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"seek": 44061, "start": 415.43, "end": 440.61, "text": "الـ Amino group هاي بتخلي .. بتعطيه rigid confirmation .. confirmation .. rigid confirmation بتاعة المركب صلابة فبيقلل ال flexibility لإنه بيخليه صعب التكسر فبدل ما يتكسر لهيدر اندانتينو الأمينو group الأناتشي تريه ويعطيني romance complex لأ صعب إنه يتكسر فإله طريقة خاصة في التفاعل بيعطيني لون yellow", "tokens": [6027, 39184, 2012, 2982, 1594, 8032, 47302, 39894, 9778, 20292, 4386, 39894, 3615, 9566, 1829, 3224, 22195, 21871, 4386, 21871, 4386, 22195, 21871, 39894, 995, 27884, 9673, 31747, 3555, 20328, 15040, 49401, 6156, 21292, 4587, 1211, 1211, 2423, 12635, 5296, 28814, 1863, 3224, 4724, 1829, 9778, 20292, 3224, 20328, 3615, 3555, 16712, 4117, 3794, 2288, 6156, 44510, 1211, 19446, 7251, 2655, 4117, 3794, 2288, 46740, 25708, 2288, 16472, 3215, 7649, 2655, 9957, 2407, 16247, 2304, 9957, 2407, 1594, 16247, 8315, 2655, 8592, 1829, 6055, 16572, 3224, 4032, 40228, 9566, 1829, 22653, 19064, 3997, 5296, 10721, 20328, 3615, 3555, 36145, 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confirmation", "probability": 0.62060546875}, {"start": 419.87, "end": 419.91, "word": " ..", "probability": 0.365234375}, {"start": 419.91, "end": 420.83, "word": " confirmation", "probability": 0.78564453125}, {"start": 420.83, "end": 420.97, "word": " ..", "probability": 0.391845703125}, {"start": 420.97, "end": 421.57, "word": " rigid", "probability": 0.8984375}, {"start": 421.57, "end": 422.29, "word": " confirmation", "probability": 0.97998046875}, {"start": 422.29, "end": 422.67, "word": " بتاعة", "probability": 0.9251302083333334}, {"start": 422.67, "end": 423.31, "word": " المركب", "probability": 0.8258463541666666}, {"start": 423.31, "end": 424.07, "word": " صلابة", "probability": 0.96337890625}, {"start": 424.07, "end": 425.19, "word": " فبيقلل", "probability": 0.8830078125}, {"start": 425.19, "end": 425.27, "word": " ال", "probability": 0.61279296875}, {"start": 425.27, "end": 425.79, "word": " flexibility", "probability": 0.9228515625}, {"start": 425.79, "end": 426.53, 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432.77, "word": " تريه", "probability": 0.7596842447916666}, {"start": 432.77, "end": 433.53, "word": " ويعطيني", "probability": 0.828515625}, {"start": 433.53, "end": 433.93, "word": " romance", "probability": 0.6806640625}, {"start": 433.93, "end": 434.53, "word": " complex", "probability": 0.89208984375}, {"start": 434.53, "end": 434.99, "word": " لأ", "probability": 0.797119140625}, {"start": 434.99, "end": 435.93, "word": " صعب", "probability": 0.9710286458333334}, {"start": 435.93, "end": 436.13, "word": " إنه", "probability": 0.82275390625}, {"start": 436.13, "end": 436.65, "word": " يتكسر", "probability": 0.99501953125}, {"start": 436.65, "end": 436.97, "word": " فإله", "probability": 0.6394856770833334}, {"start": 436.97, "end": 437.33, "word": " طريقة", "probability": 0.9903971354166666}, {"start": 437.33, "end": 437.83, "word": " خاصة", "probability": 0.97900390625}, {"start": 437.83, "end": 437.93, "word": " في", "probability": 0.955078125}, {"start": 437.93, "end": 438.47, 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take the presence of peptide bond، يدور على ال peptide bond، اذا موجودة ال peptide bond، بيعطينا positive، اذا مش موجودة، هيعطينا negative ومش هيتغير اللون", "tokens": [6027, 5172, 5016, 9381, 16712, 7649, 1829, 31439, 6156, 5016, 9381, 2423, 12198, 12, 1505, 377, 12399, 12198, 12, 1505, 377, 1975, 1829, 8592, 2423, 10024, 9154, 27188, 5016, 9381, 22807, 281, 747, 264, 6814, 295, 41781, 482, 6086, 12399, 7251, 3215, 13063, 15844, 2423, 41781, 482, 6086, 12399, 1975, 15730, 3714, 29245, 23328, 3660, 2423, 41781, 482, 6086, 12399, 4724, 1829, 3615, 9566, 1829, 8315, 3353, 12399, 1975, 15730, 37893, 3714, 29245, 23328, 3660, 12399, 39896, 3615, 9566, 1829, 8315, 3671, 4032, 2304, 8592, 8032, 36081, 17082, 13546, 13672, 11536], "avg_logprob": -0.19548233728046002, "compression_ratio": 1.7210526315789474, "no_speech_prob": 3.4570693969726562e-06, "words": [{"start": 509.89, "end": 510.29, "word": "الفحص", "probability": 0.8837890625}, {"start": 510.29, "end": 510.75, "word": " التاني", 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903.94, "word": " الأحماض", "probability": 0.8045247395833334}, {"start": 903.94, "end": 904.44, "word": " الأمينية", "probability": 0.70989990234375}, {"start": 904.44, "end": 904.84, "word": " تسميها", "probability": 0.779937744140625}, {"start": 904.84, "end": 905.3, "word": " aromatic", "probability": 0.86572265625}, {"start": 905.3, "end": 905.88, "word": " amino", "probability": 0.9814453125}, {"start": 905.88, "end": 906.28, "word": " acid", "probability": 0.974609375}, {"start": 906.28, "end": 907.02, "word": " تحتوي", "probability": 0.8518880208333334}, {"start": 907.02, "end": 907.18, "word": " على", "probability": 0.951171875}, {"start": 907.18, "end": 907.54, "word": " حلقة", "probability": 0.9876302083333334}, {"start": 907.54, "end": 908.02, "word": " بنزين", "probability": 0.9817708333333334}], "temperature": 1.0}, {"id": 41, "seek": 93073, "start": 908.67, "end": 930.73, "text": "بس مانضيف هنا إنها تكون Activated Aromatic Amino Acid تكون حلقة البنزين مشطعة أنه في حمض أمينة، حلقة البنزين اللي عنده بتكون Inactive إذا يحتوي على Aromatic Activated Aromatic Benzene Ring أو Aromatic Amino Acid بيكون .. بيعطيني لون Yellow، بيكون Positive إذا لا يحتوي، هيكون Negative", "tokens": [3555, 3794, 3714, 7649, 11242, 33911, 34105, 36145, 11296, 6055, 30544, 28550, 770, 1587, 13143, 2012, 2982, 5097, 327, 6055, 30544, 11331, 1211, 28671, 29739, 1863, 11622, 9957, 37893, 9566, 27884, 14739, 3224, 8978, 11331, 2304, 11242, 5551, 2304, 9957, 3660, 12399, 11331, 1211, 28671, 29739, 1863, 11622, 9957, 13672, 1829, 43242, 3224, 39894, 30544, 682, 12596, 11933, 15730, 7251, 33753, 45865, 15844, 1587, 13143, 28550, 770, 1587, 13143, 3964, 89, 1450, 19844, 34051, 1587, 13143, 2012, 2982, 5097, 327, 4724, 1829, 30544, 4386, 4724, 40228, 9566, 9957, 1829, 5296, 11536, 17550, 12399, 4724, 1829, 30544, 46326, 11933, 15730, 20193, 7251, 33753, 45865, 12399, 39896, 30544, 43230], "avg_logprob": -0.20052083153967504, "compression_ratio": 1.7639484978540771, "no_speech_prob": 2.4437904357910156e-06, "words": [{"start": 908.67, "end": 908.91, "word": "بس", "probability": 0.74462890625}, {"start": 908.91, "end": 909.35, "word": " مانضيف", "probability": 0.79193115234375}, {"start": 909.35, "end": 909.71, "word": " هنا", "probability": 0.62353515625}, {"start": 909.71, "end": 909.91, "word": " إنها", "probability": 0.6871337890625}, {"start": 909.91, "end": 910.25, "word": " تكون", "probability": 0.961181640625}, {"start": 910.25, "end": 911.77, "word": " Activated", "probability": 0.6387939453125}, {"start": 911.77, "end": 912.39, "word": " Aromatic", "probability": 0.757080078125}, {"start": 912.39, "end": 912.77, "word": " Amino", "probability": 0.9560546875}, {"start": 912.77, "end": 913.11, "word": " Acid", "probability": 0.881591796875}, {"start": 913.11, "end": 913.37, "word": " تكون", "probability": 0.6883544921875}, {"start": 913.37, "end": 913.75, "word": " حلقة", "probability": 0.9391276041666666}, {"start": 913.75, "end": 914.35, "word": " البنزين", "probability": 0.9573974609375}, {"start": 914.35, "end": 915.51, "word": " مشطعة", "probability": 0.7249348958333334}, {"start": 915.51, "end": 916.09, "word": " أنه", "probability": 0.6737060546875}, {"start": 916.09, "end": 916.21, "word": " في", "probability": 0.84521484375}, {"start": 916.21, "end": 916.59, "word": " حمض", "probability": 0.8538411458333334}, {"start": 916.59, "end": 917.07, "word": " أمينة،", "probability": 0.70224609375}, {"start": 917.07, "end": 917.33, "word": " حلقة", "probability": 0.9666341145833334}, {"start": 917.33, "end": 917.83, "word": " البنزين", "probability": 0.9837646484375}, {"start": 917.83, "end": 917.99, "word": " اللي", "probability": 0.913818359375}, {"start": 917.99, "end": 918.27, "word": " عنده", "probability": 0.972412109375}, {"start": 918.27, "end": 918.59, "word": " بتكون", "probability": 0.7369384765625}, {"start": 918.59, "end": 919.27, "word": " Inactive", "probability": 0.772216796875}, {"start": 919.27, "end": 920.39, "word": " إذا", "probability": 0.941650390625}, {"start": 920.39, "end": 921.05, "word": " يحتوي", "probability": 0.9754231770833334}, {"start": 921.05, "end": 921.35, "word": " على", "probability": 0.9052734375}, {"start": 921.35, "end": 922.01, "word": " Aromatic", "probability": 0.8408203125}, {"start": 922.01, "end": 922.79, "word": " Activated", "probability": 0.939453125}, {"start": 922.79, "end": 923.31, "word": " Aromatic", "probability": 0.768310546875}, {"start": 923.31, "end": 923.89, "word": " Benzene", "probability": 0.7188313802083334}, {"start": 923.89, "end": 924.17, "word": " Ring", "probability": 0.85400390625}, {"start": 924.17, "end": 924.37, "word": " أو", "probability": 0.943359375}, {"start": 924.37, "end": 924.87, "word": " Aromatic", "probability": 0.863037109375}, {"start": 924.87, "end": 925.27, "word": " Amino", "probability": 0.95068359375}, {"start": 925.27, "end": 925.91, "word": " Acid", "probability": 0.974853515625}, {"start": 925.91, "end": 926.61, "word": " بيكون", "probability": 0.709228515625}, {"start": 926.61, "end": 926.83, "word": " ..", "probability": 0.459716796875}, {"start": 926.83, "end": 927.37, "word": " بيعطيني", "probability": 0.84638671875}, {"start": 927.37, "end": 927.55, "word": " لون", "probability": 0.969482421875}, {"start": 927.55, "end": 928.09, "word": " Yellow،", "probability": 0.5753173828125}, {"start": 928.09, "end": 928.31, "word": " بيكون", "probability": 0.93798828125}, {"start": 928.31, "end": 928.81, "word": " Positive", "probability": 0.89794921875}, {"start": 928.81, "end": 929.37, "word": " إذا", "probability": 0.8818359375}, {"start": 929.37, "end": 929.57, "word": " لا", "probability": 0.9091796875}, {"start": 929.57, "end": 930.17, "word": " يحتوي،", "probability": 0.9632568359375}, {"start": 930.17, "end": 930.53, "word": " هيكون", "probability": 0.937255859375}, {"start": 930.53, "end": 930.73, "word": " Negative", "probability": 0.90283203125}], "temperature": 1.0}, {"id": 42, "seek": 95947, "start": 931.85, "end": 959.47, "text": "فكيف الـ principle مبدأ عمل الفحص أنه بيجي عنده amino acid with aromatic ring حمض أميني بيحتوي على حلقة بنزين بحط عليه concentrated nitric acid concentrated nitric acid HNO3 في وجود قاعدة قوية زي الـNaO2 في وجود NaO2 ليش هييجي يعمل الـ nitric acid لحمض الأميني اللي يحتوي على بنزين ring؟", "tokens": [5172, 4117, 33911, 2423, 39184, 8665, 3714, 44510, 10721, 6225, 42213, 27188, 5016, 9381, 14739, 3224, 4724, 1829, 7435, 1829, 43242, 3224, 24674, 8258, 365, 45831, 4875, 11331, 2304, 11242, 5551, 2304, 9957, 1829, 4724, 1829, 33753, 45865, 15844, 11331, 1211, 28671, 44945, 11622, 9957, 4724, 5016, 9566, 47356, 21321, 10900, 1341, 8258, 21321, 10900, 1341, 8258, 389, 12861, 18, 8978, 49610, 23328, 12174, 995, 22488, 3660, 12174, 2407, 10632, 30767, 1829, 2423, 39184, 33824, 46, 17, 8978, 49610, 23328, 6056, 46, 17, 32239, 8592, 39896, 1829, 7435, 1829, 7251, 25957, 1211, 2423, 39184, 10900, 1341, 8258, 5296, 35571, 11242, 16247, 2304, 9957, 1829, 13672, 1829, 7251, 33753, 45865, 15844, 44945, 11622, 9957, 4875, 22807], "avg_logprob": -0.22710129361728143, "compression_ratio": 1.7142857142857142, "no_speech_prob": 1.5497207641601562e-06, "words": [{"start": 931.85, "end": 932.35, "word": "فكيف", "probability": 0.5356648763020834}, {"start": 932.35, "end": 932.53, "word": " الـ", "probability": 0.80078125}, {"start": 932.53, "end": 932.89, "word": " principle", "probability": 0.367431640625}, {"start": 932.89, "end": 933.35, "word": " مبدأ", "probability": 0.8092447916666666}, {"start": 933.35, "end": 933.69, "word": " عمل", "probability": 0.74365234375}, {"start": 933.69, "end": 934.25, "word": " الفحص", "probability": 0.9365234375}, {"start": 934.25, "end": 934.89, "word": " أنه", "probability": 0.651123046875}, {"start": 934.89, "end": 935.31, "word": " بيجي", "probability": 0.9239501953125}, {"start": 935.31, "end": 935.97, "word": " عنده", "probability": 0.906005859375}, {"start": 935.97, "end": 936.49, "word": " amino", "probability": 0.65771484375}, {"start": 936.49, "end": 936.99, "word": " acid", "probability": 0.92919921875}, {"start": 936.99, "end": 937.31, "word": " with", "probability": 0.8095703125}, {"start": 937.31, "end": 937.77, "word": " aromatic", "probability": 0.94580078125}, {"start": 937.77, "end": 938.95, "word": " ring", "probability": 0.93798828125}, {"start": 938.95, "end": 939.91, "word": " حمض", "probability": 0.8447265625}, {"start": 939.91, "end": 940.29, "word": " أميني", "probability": 0.77545166015625}, {"start": 940.29, "end": 940.71, "word": " بيحتوي", "probability": 0.965087890625}, {"start": 940.71, "end": 940.91, "word": " على", "probability": 0.90576171875}, {"start": 940.91, "end": 941.25, "word": " حلقة", "probability": 0.978515625}, {"start": 941.25, "end": 941.69, "word": " بنزين", "probability": 0.8811848958333334}, {"start": 941.69, "end": 942.55, "word": " بحط", "probability": 0.896484375}, {"start": 942.55, "end": 942.93, "word": " عليه", "probability": 0.9580078125}, {"start": 942.93, "end": 943.87, "word": " concentrated", "probability": 0.77880859375}, {"start": 943.87, "end": 944.59, "word": " nitric", "probability": 0.96142578125}, {"start": 944.59, "end": 945.03, "word": " acid", "probability": 0.96728515625}, {"start": 945.03, "end": 946.07, "word": " concentrated", "probability": 0.1966552734375}, {"start": 946.07, "end": 946.89, "word": " nitric", "probability": 0.972412109375}, {"start": 946.89, "end": 947.19, "word": " acid", "probability": 0.98193359375}, {"start": 947.19, "end": 948.49, "word": " HNO3", "probability": 0.81982421875}, {"start": 948.49, "end": 949.11, "word": " في", "probability": 0.9013671875}, {"start": 949.11, "end": 949.69, "word": " وجود", "probability": 0.95068359375}, {"start": 949.69, "end": 950.41, "word": " قاعدة", "probability": 0.985595703125}, {"start": 950.41, "end": 951.09, "word": " قوية", "probability": 0.9905598958333334}, {"start": 951.09, "end": 951.79, "word": " زي", "probability": 0.932861328125}, {"start": 951.79, "end": 952.61, "word": " الـNaO2", "probability": 0.5451416015625}, {"start": 952.61, "end": 952.79, "word": " في", "probability": 0.8681640625}, {"start": 952.79, "end": 953.27, "word": " وجود", "probability": 0.93359375}, {"start": 953.27, "end": 954.11, "word": " NaO2", "probability": 0.8347981770833334}, {"start": 954.11, "end": 954.71, "word": " ليش", "probability": 0.59112548828125}, {"start": 954.71, "end": 955.03, "word": " هييجي", "probability": 0.8641357421875}, {"start": 955.03, "end": 955.35, "word": " يعمل", "probability": 0.9913736979166666}, {"start": 955.35, "end": 955.53, "word": " الـ", "probability": 0.798583984375}, {"start": 955.53, "end": 955.83, "word": " nitric", "probability": 0.779541015625}, {"start": 955.83, "end": 956.21, "word": " acid", "probability": 0.97607421875}, {"start": 956.21, "end": 957.51, "word": " لحمض", "probability": 0.6865234375}, {"start": 957.51, "end": 957.87, "word": " الأميني", "probability": 0.927734375}, {"start": 957.87, "end": 958.05, "word": " اللي", "probability": 0.848876953125}, {"start": 958.05, "end": 958.47, "word": " يحتوي", "probability": 0.96875}, {"start": 958.47, "end": 958.65, "word": " على", "probability": 0.90283203125}, {"start": 958.65, "end": 959.07, "word": " بنزين", "probability": 0.93505859375}, {"start": 959.07, "end": 959.47, "word": " ring؟", "probability": 0.6650390625}], "temperature": 1.0}, {"id": 43, "seek": 96291, "start": 959.71, "end": 962.91, "text": "يعمل نيترياشيل of بنزين ريكو", "tokens": [1829, 25957, 1211, 8717, 36081, 16572, 33599, 26895, 295, 44945, 11622, 9957, 12602, 1829, 4117, 2407], "avg_logprob": -0.4834558823529412, "compression_ratio": 0.9090909090909091, "no_speech_prob": 3.5762786865234375e-07, "words": [{"start": 959.71, "end": 960.27, "word": "يعمل", "probability": 0.816162109375}, {"start": 960.27, "end": 961.35, "word": " نيترياشيل", "probability": 0.573974609375}, {"start": 961.35, "end": 961.59, "word": " of", "probability": 0.625}, {"start": 961.59, "end": 962.17, "word": " بنزين", "probability": 0.9417317708333334}, {"start": 962.17, "end": 962.91, "word": " ريكو", "probability": 0.63262939453125}], "temperature": 1.0}], "language": "ar", "language_probability": 1.0, "duration": 963.7674375, "duration_after_vad": 893.2081250000015}
\ No newline at end of file
diff --git a/PL9fwy3NUQKwZ1Q7zOg1ObgufPMjRI3WY1/iXxjfqDroRk_raw.srt b/PL9fwy3NUQKwZ1Q7zOg1ObgufPMjRI3WY1/iXxjfqDroRk_raw.srt
new file mode 100644
index 0000000000000000000000000000000000000000..e02b2b385b2a372d027e0c3aa88339d64bbbac35
--- /dev/null
+++ b/PL9fwy3NUQKwZ1Q7zOg1ObgufPMjRI3WY1/iXxjfqDroRk_raw.srt
@@ -0,0 +1,920 @@
+1
+00:00:05,480 --> 00:00:09,280
+أول فحص هو الـ Nihidrine الـ Nihidrine إيش ال
+
+2
+00:00:09,280 --> 00:00:12,700
+objective لو فحصتش الهدف من الفحص to detect the
+
+3
+00:00:12,700 --> 00:00:16,300
+presence of a free alpha amino group بيدور على ال
+
+4
+00:00:16,300 --> 00:00:20,680
+free alpha amino group إذا موجود free alpha amino
+
+5
+00:00:20,680 --> 00:00:25,180
+group هيتغير اللون، بيعطيني اللون purple في حالات
+
+6
+00:00:25,180 --> 00:00:28,800
+اللي موجودة في free alpha amino groupمين عنده فيه
+
+7
+00:00:28,800 --> 00:00:31,740
+Alpha Amino Group؟ إذا ذاكرين الحمض الأميني، عيش
+
+8
+00:00:31,740 --> 00:00:35,040
+الصيغة العامة له، تكون من Center Carbon ومن
+
+9
+00:00:35,040 --> 00:00:38,640
+الهيدروجينة، من ال Side Chain ومن Carboxyl Group
+
+10
+00:00:38,640 --> 00:00:43,160
+ومن Amino Group Amino Group هاي سميناها Primary
+
+11
+00:00:43,160 --> 00:00:47,720
+Amino Group أو Alpha Amino Group، معادة في حالة
+
+12
+00:00:47,720 --> 00:00:51,920
+البرولين سميناها Amino Groupيعني الأحماض الأمينية
+
+13
+00:00:51,920 --> 00:00:57,380
+كلها تحتوي على free alpha amino group ما يعدى
+
+14
+00:00:57,380 --> 00:01:01,600
+لبرولين عنده amino group يعني مين هيعطيني positive
+
+15
+00:01:01,600 --> 00:01:04,880
+مع هذا الفحص لو أنا اشتغلت مجموعة أحماض أمينية على
+
+16
+00:01:04,880 --> 00:01:08,400
+هذا الفحص؟ كلهم هيعطوني positive لأنهم كلهم بيحتوي
+
+17
+00:01:08,400 --> 00:01:13,800
+على free alpha amino group تمام؟طيب، لذا ال
+
+18
+00:01:13,800 --> 00:01:16,260
+objective to detect the presence of free alpha
+
+19
+00:01:16,260 --> 00:01:19,360
+amino group هل موجود free alpha amino group أو لا؟
+
+20
+00:01:19,360 --> 00:01:21,940
+إذا تغير اللغة يعني موجود، إذا ما تغيرش يعني مش
+
+21
+00:01:21,940 --> 00:01:25,680
+موجود طيب إيش ال principle؟ مبدأ العمل للفحص؟ كيف
+
+22
+00:01:25,680 --> 00:01:29,120
+يشتغل الفحص؟ ال principle لو نعملها على نظام
+
+23
+00:01:29,120 --> 00:01:32,320
+معادلات بيسهل عليهكيف هي هذا الفحص الهو الـ
+
+24
+00:01:32,320 --> 00:01:35,840
+principle أنه عندنا أمينو أسد أنا بضيف حمض أميني
+
+25
+00:01:35,840 --> 00:01:38,980
+بضيف عليه نهيدرينرياجانت إيش بيعمل
+
+26
+00:01:38,980 --> 00:01:43,200
+النهيدرينرياجانت؟ بيشتغل على الـ free amino group
+
+27
+00:01:43,200 --> 00:01:47,060
+بيعطيني amino group لحالها أنا تشتريه وبيعطيني
+
+28
+00:01:47,060 --> 00:01:51,120
+هيدريندنتينإذا الأمينو أسد بضيف عليه نينهيدرين
+
+29
+00:01:51,120 --> 00:01:55,300
+بيعطيني أن أتش تري و هيدرين دانتين مركب اسمه
+
+30
+00:01:55,300 --> 00:02:00,960
+هيدرين دانتين نجيب الان أتش تري و الهيدرين دانتين
+
+31
+00:02:00,960 --> 00:02:05,100
+هاي و نحط عليهم كمان مرة نينهيدرين بيتفاعلوا مع
+
+32
+00:02:05,100 --> 00:02:08,920
+بعض و بيعطيني حاجة اسمها Romans Complex Romans
+
+33
+00:02:08,920 --> 00:02:15,420
+Complex هذا لونه purple colorإذا موجود الحنانة حمض
+
+34
+00:02:15,420 --> 00:02:18,100
+أميني، إذا كان يحتوي على Free Amino Alpha
+
+35
+00:02:18,100 --> 00:02:22,320
+Aminogram، بضيف عليه الـNahydrin Reagent، المحصلة
+
+36
+00:02:22,320 --> 00:02:25,740
+كلها أنه في الآخر هيعطيني Romans Complex،
+
+37
+00:02:25,740 --> 00:02:29,280
+الـNahydrin يشتغل على الـAmino Acid، اللي يحتوي
+
+38
+00:02:29,280 --> 00:02:32,860
+على Free Alpha Aminogram، يعطيني حاجة بنسميها
+
+39
+00:02:32,860 --> 00:02:37,440
+Romans Complex، إيش لونها؟ لون بيربونإذا إذا
+
+40
+00:02:37,440 --> 00:02:41,140
+اتغيرت تغييرت الفحص وتغير اللون ل purple إذا هاي
+
+41
+00:02:41,140 --> 00:02:46,540
+المركب يحتوي على free alpha amino group إذا هو
+
+42
+00:02:46,540 --> 00:02:50,280
+positive يحتوي على free alpha amino group وهو
+
+43
+00:02:50,280 --> 00:02:50,900
+positive
+
+44
+00:02:54,400 --> 00:02:58,660
+هنا عندنا ال principle على شكل معادلة مرسوم فيها
+
+45
+00:02:58,660 --> 00:03:01,040
+structure احنا مش مطلوب منها ال structure بس على
+
+46
+00:03:01,040 --> 00:03:04,620
+شكل معادلة حتى نلخص ال principle و يكون أثناء ال
+
+47
+00:03:04,620 --> 00:03:08,220
+principle أنه عندي حمض أميني أمينو أسد بضيف عليها
+
+48
+00:03:08,220 --> 00:03:14,020
+نهيدرين راجع بيعطيني هيدرين دنتينو Anacetrate باخد
+
+49
+00:03:14,020 --> 00:03:16,920
+الهيدرين دنتين و ال Anacetrate باطيف عليهم كمان
+
+50
+00:03:16,920 --> 00:03:20,840
+مرانين هيدرين رياجان بيعطيني حاجة بنسميها romance
+
+51
+00:03:20,840 --> 00:03:25,540
+complex لغنها purple هذا إذا كان المركب ال amino
+
+52
+00:03:25,540 --> 00:03:29,000
+acid يحتوي على free alpha amino group بيعطيني
+
+53
+00:03:29,000 --> 00:03:33,700
+romance complex لغنه purple هذه المحصلة النهائية
+
+54
+00:03:41,910 --> 00:03:45,930
+البرولين يحتوي على amino group او secondary amino
+
+55
+00:03:45,930 --> 00:03:50,030
+group لأن البرولين يتفاعل مع ال نهيدرين ال amino
+
+56
+00:03:50,030 --> 00:03:53,450
+group اللي عنده بتتفاعل مع ال نهيدرين بس لها طريقة
+
+57
+00:03:53,450 --> 00:03:57,370
+خاصة للتفاعل فبدل ما تعطيني لون purple بيعطيني لون
+
+58
+00:03:57,370 --> 00:04:02,090
+yellow لذن كل الأحماض الأمينية تعطي قيمة positive
+
+59
+00:04:02,090 --> 00:04:06,570
+مع ال نهيدرينلكن هالكلها بتعطي لون purple؟ لأ، كل
+
+60
+00:04:06,570 --> 00:04:10,230
+الأحمد الأمنية بتعطي لون purple معادا البرولين
+
+61
+00:04:10,230 --> 00:04:13,950
+بيعطيني لون yellow، زفاعل معنين هيدرين بيعطيني لون
+
+62
+00:04:13,950 --> 00:04:18,770
+yellowأمينو أسد برولين and هيدروكس برولين react
+
+63
+00:04:18,770 --> 00:04:23,290
+with ninhydrin but give yellow color من يعطيني
+
+64
+00:04:23,290 --> 00:04:26,610
+positive غير الأمينو أسد besides أمينو أسد من
+
+65
+00:04:26,610 --> 00:04:29,550
+هييعطيني positive أحمد الأمينية تعطيني positive مع
+
+66
+00:04:29,550 --> 00:04:33,070
+ال ninhydrin كمان ال peptides البروتين ال
+
+67
+00:04:33,070 --> 00:04:35,950
+structure ال primary structure of البروتين يسميه
+
+68
+00:04:35,950 --> 00:04:36,530
+peptides
+
+69
+00:04:40,710 --> 00:04:44,290
+مرتبطة مع بعضها البعض في peptide bond محماض أمينية
+
+70
+00:04:44,290 --> 00:04:47,230
+مرتبطة مع بعضها في peptide bond حكينا في جهة من
+
+71
+00:04:47,230 --> 00:04:51,030
+الجهات بيكون فيه free amino group وجهة تانية فيه
+
+72
+00:04:51,030 --> 00:04:54,450
+free carboxyl group إذا متوفر عندي free amino
+
+73
+00:04:54,450 --> 00:04:57,990
+group، يعني إذا انتوفر ال objective لإنين هيدرين،
+
+74
+00:04:57,990 --> 00:05:01,720
+يعني هيعطيني positiveالأمينو أسد هتعطيني positive
+
+75
+00:05:01,720 --> 00:05:05,540
+من النيلهيدرين ال peptides اللي هي عبارة عن ترتيب
+
+76
+00:05:05,540 --> 00:05:09,680
+من أحماض أمينية مرتبطة مع بعضها البعض ب peptide
+
+77
+00:05:09,680 --> 00:05:14,500
+bond هتعطيني برضه positive ال peptone هو عبارة عن
+
+78
+00:05:14,500 --> 00:05:17,420
+ال protein مكسّرالحين أنا لما أكسر البروتين إيش
+
+79
+00:05:17,420 --> 00:05:21,560
+هينتج عندى؟ بينتج من البروتين peptide bond و amino
+
+80
+00:05:21,560 --> 00:05:24,960
+acid بدأ من نتج amino acid و ال amino acid تحتكوي
+
+81
+00:05:24,960 --> 00:05:27,420
+على free alpha amino group هيعطيني positive مع ال
+
+82
+00:05:27,420 --> 00:05:30,620
+نينهيدرين أو لا، هيعطيني positive إذا أن ال
+
+83
+00:05:30,620 --> 00:05:35,580
+peptide و ال peptone و البروتين و ال amino acid هم
+
+84
+00:05:35,580 --> 00:05:40,160
+الأربع بيعطوني positive مع ال نينهيدرين نينهيدرين
+
+85
+00:05:40,160 --> 00:05:44,480
+هيعطيني positive معاهاطيب، أحكيلي تحت إن النهيدرين
+
+86
+00:05:44,480 --> 00:05:48,480
+فيه إيثانول وهو «flammable» قابل الإشتعال الـ
+
+87
+00:05:48,480 --> 00:05:51,780
+«regent» هذا قابل الإشتعال «toxic sam» و
+
+88
+00:05:51,780 --> 00:05:55,560
+«irritant» مهيج فبدنا نحفظه بعيد عن اللهب عشان هو
+
+89
+00:05:55,560 --> 00:05:59,120
+قابل الإشتعال، بعيد عن العينين وعن الجلد لأنه مهيج
+
+90
+00:05:59,120 --> 00:06:05,100
+وبعيد عن الملابس وبدنا نتجنب استنشاق كمية كبيرة
+
+91
+00:06:05,100 --> 00:06:08,800
+منه لأنه مهيج فبدنا نتعامل معاه بحذر
+
+92
+00:06:12,040 --> 00:06:15,700
+هنا معادلة لاتفاعل البرولين مع ال نينهيدرين حكينا
+
+93
+00:06:15,700 --> 00:06:19,480
+إله خاص يقاله طريقة خاصة لاتفاعل مع ال نينهيدرين
+
+94
+00:06:19,480 --> 00:06:22,260
+بيعطينيش romance complex اللونه purple وإنما
+
+95
+00:06:22,260 --> 00:06:26,280
+يعطيني yellow product ناتج لونه yellow إذا تفاعل
+
+96
+00:06:26,280 --> 00:06:29,820
+البرولين مع ال نينهيدرين بيعطيني لون yellow
+
+97
+00:06:33,100 --> 00:06:37,080
+هنا عنا سؤال بيحكي لي ليش كل الأحماض الأمينية with
+
+98
+00:06:37,080 --> 00:06:40,880
+all amino acid منها يدرين give purple but with
+
+99
+00:06:40,880 --> 00:06:43,120
+proline يدرين give yellow ليش ميعتنين yellow مع
+
+100
+00:06:43,120 --> 00:06:46,680
+البرولين و مع الباقي بيعطيني purple حكينا لأن
+
+101
+00:06:46,680 --> 00:06:51,520
+البرولين it have amino group but البرولين have
+
+102
+00:06:51,520 --> 00:06:56,350
+amino group not alpha amino groupالـ Amino group
+
+103
+00:06:56,350 --> 00:06:59,910
+هاي بتخلي .. بتعطيه rigid confirmation ..
+
+104
+00:06:59,910 --> 00:07:03,310
+confirmation .. rigid confirmation بتاعة المركب
+
+105
+00:07:03,310 --> 00:07:08,370
+صلابة فبيقلل ال flexibility لإنه بيخليه صعب التكسر
+
+106
+00:07:08,370 --> 00:07:11,770
+فبدل ما يتكسر لهيدر اندانتينو الأمينو group
+
+107
+00:07:11,770 --> 00:07:16,130
+الأناتشي تريه ويعطيني romance complex لأ صعب إنه
+
+108
+00:07:16,130 --> 00:07:20,110
+يتكسر فإله طريقة خاصة في التفاعل بيعطيني لون
+
+109
+00:07:20,110 --> 00:07:20,610
+yellow
+
+110
+00:07:23,370 --> 00:07:27,170
+فى ال procedure خطوات العمل انه نجيب تيوب glass
+
+111
+00:07:27,170 --> 00:07:30,030
+تيوب بحط فيها واحد مل من ال solution اللى انا
+
+112
+00:07:30,030 --> 00:07:33,390
+اعطاتكى يعنى اعطاتك محلول مجهول بديك تفحصى هل
+
+113
+00:07:33,390 --> 00:07:37,090
+يحتوي على فيه alpha منوبروب ولا لأ نحط واحد مل من
+
+114
+00:07:37,090 --> 00:07:40,510
+هذا ال solution مع خمسة دروب من ال nonhydric agent
+
+115
+00:07:41,650 --> 00:07:45,270
+و بتحطيه في ال water bath اللي بيحتوي على ماء مغلي
+
+116
+00:07:45,270 --> 00:07:50,410
+على boil water bath بحطه لمدة دقيقتهم بعد دقيقتهم
+
+117
+00:07:50,410 --> 00:07:53,970
+بطلّع ال tube من ال water bath إذا اتغير اللون ل
+
+118
+00:07:53,970 --> 00:07:58,130
+purple color إذا هذا المركب يحتوي على free alpha
+
+119
+00:07:58,130 --> 00:08:02,710
+amino group إذا مااتحولش اللون ل purple color إذا
+
+120
+00:08:02,710 --> 00:08:06,290
+لا يحتوي على free alpha amino group طب إذا أعطاني
+
+121
+00:08:06,290 --> 00:08:11,920
+yellow color إذا بعرف أنه brûléPurple color هيكون
+
+122
+00:08:11,920 --> 00:08:15,080
+أي مركب يحتوي على 3000 amino group ممكن يكون amino
+
+123
+00:08:15,080 --> 00:08:19,380
+acid، ممكن peptide، ممكن peptone، ممكن البروتين،
+
+124
+00:08:19,380 --> 00:08:24,160
+مهم يحتوي على free 1000 amino group لكن if أعطاني
+
+125
+00:08:24,160 --> 00:08:27,060
+yellow color، بعرف إنه هذا Proline
+
+126
+00:08:29,890 --> 00:08:33,650
+الفحص التاني هو فحص ال bio-retest، bio-retest ايش
+
+127
+00:08:33,650 --> 00:08:36,290
+ال objective من الفحص؟ to take the presence of
+
+128
+00:08:36,290 --> 00:08:40,290
+peptide bond، يدور على ال peptide bond، اذا موجودة
+
+129
+00:08:40,290 --> 00:08:43,490
+ال peptide bond، بيعطينا positive، اذا مش موجودة،
+
+130
+00:08:43,490 --> 00:08:48,590
+هيعطينا negative ومش هيتغير اللونإذا كيف .. وين
+
+131
+00:08:48,590 --> 00:08:51,410
+موجودة الـ peptide bond هتكون؟ هل موجودة في الحمض
+
+132
+00:08:51,410 --> 00:08:54,130
+الأميني؟ single amino acid؟ هل ال structure فيه
+
+133
+00:08:54,130 --> 00:08:58,150
+peptide bond؟ ال structure العام للأمينو أسد؟ لأ،
+
+134
+00:08:58,150 --> 00:09:01,430
+إذا أن الأمينو أسد كلها هتعطيني negative مع ال bio
+
+135
+00:09:01,430 --> 00:09:06,050
+rate test مين هيعطيني positive؟ ال protein، لأن ال
+
+136
+00:09:06,050 --> 00:09:10,010
+protein هو عبارة عن ترتيب الأحماض الأمينية مرتبطة
+
+137
+00:09:10,010 --> 00:09:13,530
+مع بعضها البعض في peptide bond فال protein هيعطيني
+
+138
+00:09:13,530 --> 00:09:20,910
+positive، كمانعندما يتكسر البروتين في بيبتايد بون،
+
+139
+00:09:20,910 --> 00:09:23,950
+فهي يعطيني positive، لذلك البروتين والبيبتون
+
+140
+00:09:23,950 --> 00:09:28,030
+هيعطوني positive قبل ال principle، المبدأ العام
+
+141
+00:09:28,030 --> 00:09:28,650
+للفحص
+
+142
+00:09:33,660 --> 00:09:37,800
+بضيف عليه bio-retriation إيش هو ال bio-retriation؟
+
+143
+00:09:37,800 --> 00:09:43,340
+مايبقى حاجته كبر صلفات with strong base كبر صلفات
+
+144
+00:09:43,340 --> 00:09:49,200
+CUSO4 في وجود strong base قاعدة قوية NaOH أو KOH
+
+145
+00:09:50,000 --> 00:09:53,660
+بيجي الـ bioretary agent الكبر اللي فيه بيروح على
+
+146
+00:09:53,660 --> 00:09:56,960
+الـ peptide هون بروبوتها كلها يتهم مع بعضها
+
+147
+00:09:56,960 --> 00:10:02,200
+ويعملهم كإنه مشبكة، بيعمل الكبر ك-chelating agent،
+
+148
+00:10:02,200 --> 00:10:06,500
+الكبر يشتغل، بيشتغل ك-chelating agent، كعامل
+
+149
+00:10:06,500 --> 00:10:11,590
+متمخلقعامل متمخلب، يعني عامل زي مخ ضبوط، إله أكتر
+
+150
+00:10:11,590 --> 00:10:14,630
+من إيه؟ في mid من هنا و من هنا و من هنا، بيروبوت
+
+151
+00:10:14,630 --> 00:10:18,390
+كل الروابط البيبجية الموجودة مع بعضها البعض، في
+
+152
+00:10:18,390 --> 00:10:23,570
+وجود القاعدة القوية، بيعمل gelating agent لكبر،
+
+153
+00:10:23,570 --> 00:10:26,670
+بيعمل gelating agent، عامل متمخلب
+
+154
+00:10:32,700 --> 00:10:37,240
+لونه violet إذا تغير اللون لviolet إذا موجود عندي
+
+155
+00:10:37,240 --> 00:10:40,780
+ال peptide bond إذا ما تغيرش إذا ال negative مش
+
+156
+00:10:40,780 --> 00:10:45,040
+موجودة بحكيلي هذا اللون this color is dependent on
+
+157
+00:10:45,040 --> 00:10:48,200
+the number of peptide bond بيعتمد على عدد ال
+
+158
+00:10:48,200 --> 00:10:51,400
+peptide bond إذا ال peptide bond موجودة عندى تنتين
+
+159
+00:10:51,400 --> 00:10:54,860
+بيكون violet بس بشدة أخف من لما يكون عندى مثلا
+
+160
+00:10:54,860 --> 00:10:59,460
+أربع روابط بيبتدية فكل ما زادت عدد روابط البيبتدية
+
+161
+00:10:59,460 --> 00:11:01,880
+كل ما زادت شدت اللون
+
+162
+00:11:06,510 --> 00:11:09,350
+في الـ Reagent البيوريد حاكينا بيحتوي على كبر
+
+163
+00:11:09,350 --> 00:11:14,030
+صلفات with a strong base NaOH أو KOH ليش موجودة
+
+164
+00:11:14,030 --> 00:11:17,730
+الـ strong base الـ NaOH is there to raise the pH
+
+165
+00:11:17,730 --> 00:11:22,530
+عشان ترفع الـ pH لتوفير alkaline media لتوفير
+
+166
+00:11:22,530 --> 00:11:26,330
+alkaline media الـ pH اللي نحتاجها لتوفير alkaline
+
+167
+00:11:26,330 --> 00:11:30,870
+media طيب الميني المركب المهم في هذه الـ في هذا
+
+168
+00:11:30,870 --> 00:11:31,930
+الـ Reagent الـ circular
+
+169
+00:11:39,380 --> 00:11:43,180
+المركب المهم هو الـ cover ال cover حاكينا هو أهم
+
+170
+00:11:43,180 --> 00:11:47,440
+مركب موجود في ال region بيجي من ال cover صلفية في
+
+171
+00:11:47,440 --> 00:11:51,740
+وجود alkaline solution إذا وجدت ال peptide bond و
+
+172
+00:11:51,740 --> 00:11:54,660
+ال peptide bond are present ال alkaline solution
+
+173
+00:11:54,660 --> 00:11:58,220
+ال cover من ال cover صلفية بيجي بيعطيني complex
+
+174
+00:11:58,220 --> 00:12:03,600
+لونه violetبيحكيلي إنه two peptide bonds at least
+
+175
+00:12:03,600 --> 00:12:07,220
+are required for the formation of this complex على
+
+176
+00:12:07,220 --> 00:12:11,660
+الأقل يكون موجود في المُركّب two peptide bonds إذا
+
+177
+00:12:11,660 --> 00:12:15,660
+كان موجود رابطتين ببتديات أو أكتر بيشتغل ال
+
+178
+00:12:15,660 --> 00:12:19,620
+reagent وبيعطيني لهم إذا رابطة ببتدية واحدة بيقدرش
+
+179
+00:12:19,620 --> 00:12:23,000
+يشتغل ويعطيني negative لأنه لو أجل كبر على رابطة
+
+180
+00:12:23,000 --> 00:12:26,260
+ببتدية واحدةالكيب دي عمل عامل متمخلب وبإنه بدي
+
+181
+00:12:26,260 --> 00:12:29,760
+ربطهم كلهم مع بعض مافيش غير رابط واحد يربطها مع
+
+182
+00:12:29,760 --> 00:12:33,580
+مين فمش هيعطيني لون فعلى الأقل لازم يكون موجود
+
+183
+00:12:33,580 --> 00:12:35,260
+رابطتين فأكثر
+
+184
+00:12:38,260 --> 00:12:41,960
+إيش هو الكليات؟ الكليات هو chemical compound مركب
+
+185
+00:12:41,960 --> 00:12:47,320
+كيميائي بيتكون من metal ion مع كليات انجازان مركب
+
+186
+00:12:47,320 --> 00:12:52,380
+بيتكون من metal ion مع كليات انجازان طب مين الكلية
+
+187
+00:12:52,380 --> 00:12:55,820
+انجازان؟ هو ال substance whose molecule can form
+
+188
+00:12:55,820 --> 00:13:00,040
+several bonds to a single metal ion هو المركب اللي
+
+189
+00:13:00,040 --> 00:13:06,060
+له ID كثيرة هو المركب القادر على عمل عدة روابط
+
+190
+00:13:15,860 --> 00:13:19,240
+إيش الـ procedure خطوات عمل الفحص أني بجيب الـ
+
+191
+00:13:19,240 --> 00:13:21,820
+tube بحط فيها واحد مل من ال solution اللي أنا
+
+192
+00:13:21,820 --> 00:13:25,320
+أعطاتكيها سواء أعطاتكي protein أو amino acid تحطي
+
+193
+00:13:25,320 --> 00:13:29,420
+واحد منه على اتنين مل من ال bioretarian بتحطي
+
+194
+00:13:29,420 --> 00:13:33,920
+بتعملي mix إذا تغير اللون ل violet إذا هذا المركب
+
+195
+00:13:33,920 --> 00:13:37,400
+اللي أنا أعطاتكيه يحتوي على peptide bond إذا ما
+
+196
+00:13:37,400 --> 00:13:44,430
+تغيرش إذا ال negative له يحتوي على peptide bondطيب
+
+197
+00:13:44,430 --> 00:13:47,990
+هنا جدول يحتوي على الألوان اللي ممكن تظهر لما أنا
+
+198
+00:13:47,990 --> 00:13:50,990
+أشتغل فحص ال بيرون، بحكيلي if a note changed،
+
+199
+00:13:50,990 --> 00:13:54,170
+الفحص هو لون ال بيروترياجنت الطبيعي، يعني لونه
+
+200
+00:13:54,170 --> 00:13:57,990
+أزرق، فإذا كان negative هيظل لون أزرق زي ما هو،
+
+201
+00:13:57,990 --> 00:14:01,810
+إذا positive هيتغير لviolinفإذا ما تغيرش اللون
+
+202
+00:14:01,810 --> 00:14:05,230
+وضلوا أزرق، معناها البروتين مش موجود يعني ممكن
+
+203
+00:14:05,230 --> 00:14:08,370
+يكون موجود عندي حمض أمين، المهم الـbutyde bond مش
+
+204
+00:14:08,370 --> 00:14:13,110
+موجودة، فاللون ما تغيرش، ماصارش تفاعل إذا اللون
+
+205
+00:14:13,110 --> 00:14:17,470
+اتغير، بيحكيلي إلى violet من blue لviolet لدرجة ال
+
+206
+00:14:17,470 --> 00:14:21,710
+purple، الغامق، إذا البروتين موجود طب إذا اتغير لل
+
+207
+00:14:21,710 --> 00:14:25,930
+pink لدرجة أفتح من الviolet، اللون pink، إذا اللي
+
+208
+00:14:25,930 --> 00:14:30,900
+موجود بـbutyde bond، ولكن عددها قليل، أقليعني ممكن
+
+209
+00:14:30,900 --> 00:14:35,280
+يكون موجود وعندي بيبتون اللي هو عند بروتين مكسّة،
+
+210
+00:14:35,280 --> 00:14:39,460
+عدد البيبتايد فوق الـ bond اللي فيه قليل، فدرجة
+
+211
+00:14:39,460 --> 00:14:40,980
+اللون بتكون أقل.
+
+212
+00:14:43,720 --> 00:14:48,300
+البحث اللي بعد هيك هو الـ xanthoproteic test الـ
+
+213
+00:14:48,300 --> 00:14:51,140
+xanthoproteic test إيش ال objective منه؟ to
+
+214
+00:14:51,140 --> 00:14:55,720
+differentiate between aromatic and amino acidلأ،
+
+215
+00:14:55,720 --> 00:14:59,320
+بس ده الـ amino acid اللي بيحتوي على benzene ring
+
+216
+00:14:59,320 --> 00:15:02,660
+على حلقة بنزين، إذا فاكرين الصورة دي اللي حكينا
+
+217
+00:15:02,660 --> 00:15:05,880
+إنه في بعض الأحماض الأمينية تسميها aromatic amino
+
+218
+00:15:05,880 --> 00:15:10,250
+acid تحتوي على حلقة بنزينبس مانضيف هنا إنها تكون
+
+219
+00:15:10,250 --> 00:15:14,350
+Activated Aromatic Amino Acid تكون حلقة البنزين
+
+220
+00:15:14,350 --> 00:15:18,270
+مشطعة أنه في حمض أمينة، حلقة البنزين اللي عنده
+
+221
+00:15:18,270 --> 00:15:22,790
+بتكون Inactive إذا يحتوي على Aromatic Activated
+
+222
+00:15:22,790 --> 00:15:26,610
+Aromatic Benzene Ring أو Aromatic Amino Acid بيكون
+
+223
+00:15:26,610 --> 00:15:29,570
+.. بيعطيني لون Yellow، بيكون Positive إذا لا
+
+224
+00:15:29,570 --> 00:15:33,690
+يحتوي، هيكون Negativeفكيف الـ principle مبدأ عمل
+
+225
+00:15:33,690 --> 00:15:38,950
+الفحص أنه بيجي عنده amino acid with aromatic ring
+
+226
+00:15:38,950 --> 00:15:42,930
+حمض أميني بيحتوي على حلقة بنزين بحط عليه
+
+227
+00:15:42,930 --> 00:15:47,190
+concentrated nitric acid concentrated nitric acid
+
+228
+00:15:47,190 --> 00:15:54,110
+HNO3 في وجود قاعدة قوية زي الـNaO2 في وجود NaO2
+
+229
+00:15:54,110 --> 00:15:58,050
+ليش هييجي يعمل الـ nitric acid لحمض الأميني اللي
+
+230
+00:15:58,050 --> 00:16:02,910
+يحتوي على بنزين ring؟يعمل نيترياشيل of بنزين ريكو
+
diff --git a/PL9fwy3NUQKwZ1Q7zOg1ObgufPMjRI3WY1/nTcz4I3nh3A.srt b/PL9fwy3NUQKwZ1Q7zOg1ObgufPMjRI3WY1/nTcz4I3nh3A.srt
new file mode 100644
index 0000000000000000000000000000000000000000..e90f2d56944df375c51865215f4d7a58d28a29f5
--- /dev/null
+++ b/PL9fwy3NUQKwZ1Q7zOg1ObgufPMjRI3WY1/nTcz4I3nh3A.srt
@@ -0,0 +1,1466 @@
+1
+00:00:01,890 --> 00:00:06,410
+اليوم راح نحكي بمعمل هو isolation of casein from 
+
+2
+00:00:06,410 --> 00:00:10,890
+milk اللي هو عزل الـ protein الـ casein من الحليب
+
+3
+00:00:10,890 --> 00:00:15,770
+وهذا يعتبر تطبيق على المعمل الماضي إن أحنا صارنا 
+
+4
+00:00:15,770 --> 00:00:19,710
+عارفين الـ isoelectric point للـ casein بدنا نوصل
+
+5
+00:00:19,710 --> 00:00:24,150
+الحليب للـ isoelectric point بتبعت الـ casein
+
+6
+00:00:24,150 --> 00:00:29,350
+وبالتالي بيترسب الـ protein الـ casein و تبقى باقي
+
+7
+00:00:29,350 --> 00:00:30,410
+الـ proteinات
+
+8
+00:00:33,330 --> 00:00:37,510
+في البداية بدنا نحكي عن الـ protein أنه ينقسم إلى
+
+9
+00:00:37,510 --> 00:00:40,830
+قسمين نوع الـ protein في simple protein و في
+
+10
+00:00:40,830 --> 00:00:45,530
+conjugated protein بروتينات بسيطة أو بروتينات 
+
+11
+00:00:45,530 --> 00:00:52,330
+معقدة simple protein هي عبارة عن أحماض أمينية
+
+12
+00:00:52,330 --> 00:00:56,750
+مرتبطة مع بعضها البعض بروابط ببتيدية يعني لو كسرتها
+
+13
+00:00:56,750 --> 00:01:01,030
+hydrolyzed to yield only amino acid زي الألبومين 
+
+14
+00:01:01,030 --> 00:01:04,690
+والجلوبيلين لو كسرتها بس منتج منها أحماض أمينية
+
+15
+00:01:04,690 --> 00:01:09,260
+مثال عليها الألبومين والجلوبيلين conjugated protein
+
+16
+00:01:09,260 --> 00:01:13,780
+بروتينات معقدة اللي بتحتوي على أحماض أمنية ومتصل
+
+17
+00:01:13,780 --> 00:01:18,820
+فيها another group other chemical group  بروتين 
+
+18
+00:01:18,820 --> 00:01:21,440
+that function in interaction with other chemical 
+
+19
+00:01:21,440 --> 00:01:24,940
+group attached by covalent bond or by weak 
+
+20
+00:01:24,940 --> 00:01:28,780
+interaction المتصل مع الأحماض الأمنية مجموعة تانية
+
+21
+00:01:28,780 --> 00:01:32,240
+مجموعة group تانية ممكن تكون هذه المجموعة في صفية 
+
+22
+00:01:32,240 --> 00:01:36,220
+group ممكن تكون carbohydrate ممكن والـ lipid ممكن 
+
+23
+00:01:36,220 --> 00:01:39,740
+مجموعة هيم فعش ما نسميها أو some example of
+
+24
+00:01:39,740 --> 00:01:43,400
+conjugated protein هو زي الـ lipoprotein اللي هو 
+
+25
+00:01:43,400 --> 00:01:47,620
+protein متصل فيه مجموعة lipid الـ glycoprotein
+
+26
+00:01:47,620 --> 00:01:51,920
+اللي هو protein متصل فيه carbohydrate سكريات الـ
+
+27
+00:01:51,920 --> 00:01:54,660
+phosphoprotein هو عبارة عن protein مرتبط فيه 
+
+28
+00:01:54,660 --> 00:01:59,300
+مجموعة فوسفان والـ hemoprotein هو protein متصل فيه 
+
+29
+00:01:59,570 --> 00:02:04,010
+مجموعة heme إذا كانت أحماض أمينية متصلة معها group 
+
+30
+00:02:04,010 --> 00:02:08,290
+تانية ساميها conjugated protein إذا كان بس أحماض
+
+31
+00:02:08,290 --> 00:02:12,510
+أمينية متصلة مع بعض البعض بسميه simple protein
+
+32
+00:02:15,400 --> 00:02:18,960
+ممكن نحكي عن الـ Phosphoprotein حكينا إن هو عبارة
+
+33
+00:02:18,960 --> 00:02:22,500
+عن بروتين أو عن أحماض أمينية bonded to a substance
+
+34
+00:02:22,500 --> 00:02:25,540
+containing phosphoric acid متصلة فيه مجموعة 
+
+35
+00:02:25,540 --> 00:02:30,040
+phosphate group أو أي مركّب مادة تحتوي على
+
+36
+00:02:30,040 --> 00:02:33,540
+phosphoric acid إذا كان موجود مجموعة phosphate
+
+37
+00:02:33,540 --> 00:02:38,870
+بسميه phosphoprotein لو جبنا أيونات الكالسيوم 
+
+38
+00:02:38,870 --> 00:02:42,310
+أيونات الكالسيوم معروفة أنها بيكون plus والفوسفات
+
+39
+00:02:42,310 --> 00:02:46,370
+جروب الفوسفات تكون minus إذا حطينا أيونات
+
+40
+00:02:46,370 --> 00:02:50,890
+الكالسيوم على بروتين يحتوي على فوسفات جروب بيصير
+
+41
+00:02:50,890 --> 00:02:56,190
+عملية ionization تأيّن للفوسفات جروب موجب مع سلب
+
+42
+00:02:56,190 --> 00:03:00,370
+الكلسيوم موجب و الفوسفات سلب فبأي نوع الكالسيوم
+
+43
+00:03:00,370 --> 00:03:04,070
+بأيّن الفوسفات تصير شحنات الموجب والسلبة متساوية
+
+44
+00:03:04,070 --> 00:03:07,660
+الشحنة الكل هي zero فهذا بيزود الـ hydrophobic
+
+45
+00:03:07,660 --> 00:03:13,460
+interaction بيزود أنه يكون كاره للماء أو أنه يكون
+
+46
+00:03:13,460 --> 00:03:17,820
+غير قطبي فهذا بيأد إلى ال precipitation ionized
+
+47
+00:03:17,820 --> 00:03:20,920
+phosphate group by calcium ion increase
+
+48
+00:03:20,920 --> 00:03:24,040
+hydrophobic interaction which lead to 
+
+49
+00:03:24,040 --> 00:03:27,680
+precipitation إذا جيبت أيانات الكالسيوم، بحطها على 
+
+50
+00:03:27,680 --> 00:03:31,200
+بروتين يحتوي على فوسفات، تكون الكالسيوم موجب 
+
+51
+00:03:31,200 --> 00:03:35,720
+والفوسفات minus، بتأين الشحنات، بتعادلوا يعني،
+
+52
+00:03:35,720 --> 00:03:39,380
+الشحنات الموجبة أو السالبة، بزود الـhydrophobic، و
+
+53
+00:03:39,380 --> 00:03:44,900
+بيصير ال precipitation بترسب هذا البروتين طيب هنا
+
+54
+00:03:44,900 --> 00:03:48,160
+الصورة بتوضح الـ milk composition اللي هي مكونات 
+
+55
+00:03:48,160 --> 00:03:51,460
+الحليب ليش بتكون الحليب؟ بيتكون من carbohydrates
+
+56
+00:03:51,460 --> 00:03:56,400
+بيحتوي على سكريات و هو سكر اللاكتوز يحتوي على واطر
+
+57
+00:03:56,400 --> 00:04:00,020
+على ميه وهي أكبر نسبة في الحليب هي ميه منيرال 
+
+58
+00:04:00,020 --> 00:04:03,660
+بيحتوي على منيرال على معادن زي الكالسيوم بوتاسيوم 
+
+59
+00:04:03,660 --> 00:04:08,440
+صوديوم فوسفورس يحتوي على فيتامين اللي هي فيتامين
+
+60
+00:04:08,440 --> 00:04:14,260
+A وD وK بيحتوي على proteins زي بروتين الكازين 
+
+61
+00:04:14,260 --> 00:04:20,790
+بيحتوي على lipid على دهون زي ما حكينا تكرار إن 
+
+62
+00:04:20,790 --> 00:04:25,530
+الحليب يتكون من فيتامين، فيتامين A وD وK، يتكون من
+
+63
+00:04:25,530 --> 00:04:28,590
+مينيرال زي الكالسيوم، بوتاسيوم، صوديوم، and
+
+64
+00:04:28,590 --> 00:04:32,570
+فوسفرس، يتكون من البروتين، و البروتين هذا يحتوي
+
+65
+00:04:32,570 --> 00:04:35,690
+على كل الأحمض الأمينية الأساسية، all essential
+
+66
+00:04:35,690 --> 00:04:38,570
+amino acid، يعني كل الأحمض الأمينية الأساسية
+
+67
+00:04:38,570 --> 00:04:42,070
+موجودة في الحليب، يحتوي على carbohydrates، على 
+
+68
+00:04:42,070 --> 00:04:46,440
+سكريات، وأشهرها، و السكر الموجود في الحليب هو سكر 
+
+69
+00:04:46,440 --> 00:04:51,460
+اللاكتوز وهو عبارة عن disaccharide سكر ثنائي عبارة 
+
+70
+00:04:51,460 --> 00:04:55,620
+عن اتحاد سكر الـ Galactose مع الـ Glucose ويحتوي
+
+71
+00:04:55,620 --> 00:05:02,010
+على لبن أهم عنصرين مش موجودين في الحليب مفقدين هو 
+
+72
+00:05:02,010 --> 00:05:06,750
+الحديد و vitamin C إذا الحليب يعتبر مغذ رئيسي 
+
+73
+00:05:06,750 --> 00:05:11,670
+يحتوي على مكونات كتيرة يحتاجها للجسم لكن في عنصرين
+
+74
+00:05:11,670 --> 00:05:15,450
+أساسيا رئيسيا يحتاجه الجسم و غير موجودة في الحليب
+
+75
+00:05:18,800 --> 00:05:22,540
+in which milk is deficient are iron and vitamin C
+
+76
+00:05:22,540 --> 00:05:26,720
+هو الحديد و vitamin C طب بيحكي للبعبي الـ infants
+
+77
+00:05:26,720 --> 00:05:30,640
+are usually born كيف الرضع الرضع بيعتمد بس على 
+
+78
+00:05:30,640 --> 00:05:33,940
+حليب الأم بياخد كل الأشياء الأساسية طب من وين
+
+79
+00:05:33,940 --> 00:05:37,960
+بيجيب الحديد و من وين بيجيب vitamin C بيحكي للحديد 
+
+80
+00:05:37,960 --> 00:05:43,350
+موجود في جسمه منذ الولادة بكمية تكفيه لعدة أسابيع
+
+81
+00:05:43,350 --> 00:05:48,390
+عنده مخزن born with a storage supply of iron large 
+
+82
+00:05:48,390 --> 00:05:52,130
+enough to meet their need for several weeks إنه 
+
+83
+00:05:52,130 --> 00:05:56,050
+عنده مخزن في جسمه حديد كميات كبيرة تكفيه لعدة
+
+84
+00:05:56,050 --> 00:06:00,650
+أسابيع أما vitamin C سهل الحصول عليه عن طريق أنه 
+
+85
+00:06:00,650 --> 00:06:06,810
+تتناول الأم عصير البرتقال وبيتزود في الحليب للطفل
+
+86
+00:06:09,270 --> 00:06:12,530
+الواحد البروتينات الموجودة في الحليب في أن تلت 
+
+87
+00:06:12,530 --> 00:06:16,730
+أنواع من البروتينات موجودة في الحليب هو الكازين،
+
+88
+00:06:16,730 --> 00:06:21,610
+lactalbumin و lactoglobulin البروتين الكازين بيشكل 
+
+89
+00:06:21,610 --> 00:06:26,390
+في الحليب نسبة 80% من البروتينة أما lactalbumin و
+
+90
+00:06:26,390 --> 00:06:31,490
+lactoglobulin بتسميهم واي بروتين تشكل 20% من نسبة
+
+91
+00:06:31,490 --> 00:06:35,430
+البروتينات الموجودة في الحليب فالنسبة الكبرى
+
+92
+00:06:35,430 --> 00:06:37,610
+للبروتينات هي للكازين
+
+93
+00:06:39,720 --> 00:06:43,680
+ممكن نتعرف على الـ casein، structure of casein الـ
+
+94
+00:06:43,680 --> 00:06:48,300
+casein يعتبر phosphoprotein، إذا هو conjugated
+
+95
+00:06:48,300 --> 00:06:52,720
+protein، not simply protein، هو conjugate مثلًا
+
+96
+00:06:52,720 --> 00:06:55,800
+على phosphoprotein، وهو conjugate، ليش؟ بتتصل فيه
+
+97
+00:06:55,800 --> 00:06:59,380
+مجموعة phosphate group attached to some of the 
+
+98
+00:06:59,380 --> 00:07:02,940
+amino acid مرتبطة مع بعض الأحمض الأمينية الموجودة
+
+99
+00:07:02,940 --> 00:07:07,210
+فيه أي أحماض أمينية مرتبطة فيها الـ Phosphate Group
+
+100
+00:07:07,210 --> 00:07:12,670
+غالباً عند الحمض الأميني سيرين وثريونين مرتبطة
+
+101
+00:07:12,670 --> 00:07:15,610
+مجموعة الـ Phosphate Group في الحمض الأميني
+
+102
+00:07:15,610 --> 00:07:20,860
+السيرين وثريونين الموجودين في تركيبة الكازين بحكي
+
+103
+00:07:20,860 --> 00:07:24,580
+لي إن الكازين هو mixture of at least three similar
+
+104
+00:07:24,580 --> 00:07:30,140
+proteins وعبارة عن خليط من ثلاث بروتينات الكازين هو
+
+105
+00:07:30,140 --> 00:07:34,020
+خليط من ثلاث بروتينات هذه الثلاث بروتينات الفرق
+
+106
+00:07:34,020 --> 00:07:38,540
+بينها بتختلف هما متشابهين بس بيختلفوا في الـ
+
+107
+00:07:38,540 --> 00:07:43,600
+molecular weight الوزن الجزائي بتختلف في كمية الـ 
+
+108
+00:07:43,600 --> 00:07:46,900
+phosphorus اللي بتحتويها number of phosphate group
+
+109
+00:07:46,900 --> 00:07:48,420
+amount of phosphorus
+
+110
+00:07:51,160 --> 00:07:54,460
+بينهم، اللي هي كمية الـ Phosphate group و الـ
+
+111
+00:07:54,460 --> 00:07:58,280
+Molecular weight إيش هم الثلاث بروتينات؟ هم 
+
+112
+00:07:58,280 --> 00:08:04,120
+Alpha-Casein و Beta-Casein و Kappa-Casein ثلاث،
+
+113
+00:08:04,120 --> 00:08:08,420
+Alpha-Casein و Beta-Casein و Kappa-Casein بحكيلي
+
+114
+00:08:08,420 --> 00:08:12,180
+هدول الثلاث كمان اللي هم different hydrophobic
+
+115
+00:08:12,180 --> 00:08:15,640
+and hydrophilic regions بختلف اللي هو 
+
+116
+00:08:20,490 --> 00:08:23,670
+في منها كاره للماء، في منها محب للماء، في منها
+
+117
+00:08:23,670 --> 00:08:29,390
+بيذوب في الماء، وفي لا نيجي 
+
+118
+00:08:29,390 --> 00:08:33,090
+نحكي عن الثلاث بروتينات المقاومة للكازين Alpha 
+
+119
+00:08:33,090 --> 00:08:36,890
+-Casein هو الـ major casein هو الأكبر أعلى 
+
+120
+00:08:36,890 --> 00:08:40,350
+molecular weight أعلى من بيتا و أعلى من كابا 
+
+121
+00:08:40,350 --> 00:08:44,950
+بيتكون من ثمانية إلى عشرة فوسفات جروب الـ Alpha 
+
+122
+00:08:44,950 --> 00:08:50,220
+-Casein هو أكتر هو الـ major هو الـ highest
+
+123
+00:08:50,220 --> 00:08:53,020
+molecular weight عدد الـ phosphate group فيه من
+
+124
+00:08:53,020 --> 00:08:57,620
+ثمانية لعشرة الـ beta casein هو أقل molecular
+
+125
+00:08:57,620 --> 00:09:01,260
+weight من ألفا وأعلى من كابا molecular weight له 
+
+126
+00:09:01,260 --> 00:09:06,080
+أقل من ألفا وأعلى من كابا بيتكون من خمسة phosphate 
+
+127
+00:09:06,080 --> 00:09:10,610
+group متصلة فيه بحكيلي الـ Beta-Casein more 
+
+128
+00:09:10,610 --> 00:09:14,910
+hydrophobic than Alpha-Casein and Kappa-Casein الـ 
+
+129
+00:09:14,910 --> 00:09:20,290
+Beta-Casein أعلى هيدروفوبك من Alpha ومن Kappa الـ
+
+130
+00:09:20,290 --> 00:09:24,410
+Beta أعلى هيدروفوبك كاره للماء أكتر من Alpha و
+
+131
+00:09:24,410 --> 00:09:28,750
+أكتر من Kappa ليش؟ لأن الأحماض الأمينية المكونة
+
+132
+00:09:28,750 --> 00:09:33,650
+إلي أغلبها non-polar أغلبها هيدروفوبك كاره للماء
+
+133
+00:09:34,660 --> 00:09:38,960
+حكيلي، بسبب أن ألفا كازين وبيتا كازين مرتبطين
+
+134
+00:09:38,960 --> 00:09:45,860
+بالكالسيوم هم مرتبطين بالكالسيوم لأنهم مرتبطين
+
+135
+00:09:45,860 --> 00:09:50,240
+بالكالسيوم لأنهم مرتبطين بالكالسيوم لأنهم مرتبطين
+
+136
+00:09:50,240 --> 00:09:53,080
+بالكالسيوم لأنهم مرتبطين بالكالسيوم لأنهم مرتبطين
+
+137
+00:09:53,080 --> 00:09:54,960
+بالكالسيوم لأنهم مرتبطين بالكالسيوم لأنهم مرتبطين
+
+138
+00:09:54,960 --> 00:09:57,260
+بالكالسيوم لأنهم مرتبطين بالكالسيوم لأنهم مرتبطين
+
+139
+00:09:57,260 --> 00:10:00,740
+بالكالسيوم لأنهم مرتبطين بالكالسيوم لأنهم مرتبطين
+
+140
+00:10:00,740 --> 00:10:04,480
+بالكالسيوم لأنهم مرتبطين بالكالسيوم لأنهم مرتبطين
+
+141
+00:10:04,480 --> 00:10:09,090
+بالكحطيت عليهم كالسيوم، هيترسبه، ليش؟ لأنه بيصير
+
+142
+00:10:09,090 --> 00:10:13,110
+ionization للفوسفات، ووجود الكالسيوم، فبيصير
+
+143
+00:10:13,110 --> 00:10:17,770
+مترسب، بيبطل soluble، بيصير insoluble زي المعادل
+
+144
+00:10:17,770 --> 00:10:20,670
+اللي تحت مكتوب، أن البروتين موطنه لسه فيه 
+
+145
+00:10:20,670 --> 00:10:23,850
+phosphate group، يعني phosphoprotein، زائد 
+
+146
+00:10:23,850 --> 00:10:27,190
+كالسيوم، لو ضفنا عليه كالسيوم، بيصير ionization
+
+147
+00:10:27,190 --> 00:10:30,930
+للفوسفات في وجود الكالسيوم، فبيصير insoluble
+
+148
+00:10:34,010 --> 00:10:39,450
+طب بالنسبة لـ كابا كازين الكابا كازين مش زي Alpha و
+
+149
+00:10:39,450 --> 00:10:43,470
+Beta كازين يحتوي فقط هو أقل Molecular weight و
+
+150
+00:10:43,470 --> 00:10:46,930
+يحتوي فقط على 2 Phosphate Group بس على 2 Phosphate 
+
+151
+00:10:46,930 --> 00:10:51,270
+Group النسبة الأكبر فيه هي Carbohydrate هي التكوين
+
+152
+00:10:51,270 --> 00:10:56,910
+من Carbohydrate مليء بالسكر الكابا كازين مش زي
+
+153
+00:10:56,910 --> 00:11:02,270
+Alpha و Beta كازين يحتوي فقط هو أقل يحتوي فقط على 
+
+154
+00:11:02,270 --> 00:11:04,710
+two phosphate group بس على اثنين من الـ phosphate
+
+155
+00:11:04,710 --> 00:11:09,550
+group النسب الأكبر فيه هي carbohydrate التكون من 
+
+156
+00:11:09,550 --> 00:11:14,950
+carbohydrate مليء بالسكر فبسموه glycoprotein
+
+157
+00:11:14,950 --> 00:11:19,890
+الكابا كازين هو glycoprotein يحتوي على سكر ياد فهو 
+
+158
+00:11:19,890 --> 00:11:24,530
+hydrophilic They are stable in the presence of
+
+159
+00:11:24,530 --> 00:11:28,250
+calcium لأنه فيه بس اثنين فوسفات جروب أما الأغلب
+
+160
+00:11:28,250 --> 00:11:32,310
+هو سكريات فهو stable هو ثابت في وجود الكالسيوم
+
+161
+00:11:32,310 --> 00:11:36,830
+مابتأثرش في الكالسيوم زي الألفة والبيتا لو حصلهم
+
+162
+00:11:36,830 --> 00:11:40,610
+كالسيوم حاجة بيصيروا insoluble بيترسبوا لأنه عندهم
+
+163
+00:11:40,610 --> 00:11:43,910
+فوسفات جروب بيصيرلها ionized بالكالسيوم مابترسبوا 
+
+164
+00:11:43,910 --> 00:11:48,290
+لكن الكابا كازين لأن كمية الفوسفات عنده أقل
+
+165
+00:11:48,290 --> 00:11:51,920
+وإنما كمية الكاربوهيدرات السكر هي اللي عالية أنا لو 
+
+166
+00:11:51,920 --> 00:11:56,500
+حطيت calcium مابيتأثر، بيكون steric وهذا بيلعب دور 
+
+167
+00:11:56,500 --> 00:12:00,520
+في حماية الـ alpha و الـ beta كازين 
+
+168
+00:12:06,820 --> 00:12:10,580
+أن هما المفروض يكونوا مترسبين مش ضايبين في الحليب 
+
+169
+00:12:10,580 --> 00:12:13,880
+بس احنا لما نشوف الحليب هل بنشوف فيه حاجة مترسبة
+
+170
+00:12:13,880 --> 00:12:17,880
+تحت ولا كله خليط مع بعض، بنشوفه كله خليط، مانشوفش
+
+171
+00:12:17,880 --> 00:12:20,340
+أنه فيه حاجة مترسبة تحت، يعني Alpha و Beta ما
+
+172
+00:12:20,340 --> 00:12:23,180
+بيكونوا مترسبين، ليش Alpha و Beta ما بيكونوا
+
+173
+00:12:23,180 --> 00:12:27,760
+مترسبين؟ لأن الكابا كازين التي لا تتأثر في وجود
+
+174
+00:12:27,760 --> 00:12:33,380
+الكالسيوم تعمل على حماية Alpha و Beta من الترسب
+
+175
+00:12:33,840 --> 00:12:37,820
+they protecting play a role in protecting other
+
+176
+00:12:37,820 --> 00:12:41,460
+casein from precipitation and make casein more
+
+177
+00:12:41,460 --> 00:12:46,220
+soluble كيف بتكوين حاجة اسمها الـ micelles؟ كيف
+
+178
+00:12:46,220 --> 00:12:49,720
+بتحمي Alpha و Beta بتكوين حاجة بتسميها الـ
+
+201
+00:14:25,020 --> 00:14:30,560
+المركبات الكبيرة للـ  Very Very Large Molecules الـ
+
+202
+00:14:30,560 --> 00:14:32,800
+Very Very Large Molecules
+
+203
+00:14:37,840 --> 00:14:41,800
+أما المركبات الكبيرة، حقيقة هي كبيرة جدا أنها تكون
+
+204
+00:14:41,800 --> 00:14:44,380
+ضائبة في الماء، المركبات الكبيرة كيف بدأت تدوب في 
+
+205
+00:14:44,380 --> 00:14:49,280
+الماء، بل هذه الجزيئات الكبيرة ستصبح مصطلحات تسمح
+
+206
+00:14:49,280 --> 00:14:51,880
+لهم بالدخول في الماء
+
+207
+00:15:06,030 --> 00:15:10,890
+ماء لأنها مركبات كبيرة فتوزيع هذه الـ large
+
+208
+00:15:10,890 --> 00:15:14,590
+structure in water dispersion أو انتشار this large
+
+209
+00:15:14,590 --> 00:15:18,570
+molecule structure in water is known as a colloidal
+
+210
+00:15:18,570 --> 00:15:22,410
+suspension بنسميها توزيع هذه المركب الكبير اللي
+
+211
+00:15:22,410 --> 00:15:26,230
+هي القعبة كازين برا ال فوبيتا بتكون في الداخل
+
+212
+00:15:26,230 --> 00:15:29,670
+فهدولة كون مركب كامل كأنه دائرة جواها
+
+213
+00:15:35,820 --> 00:15:40,240
+ماشى فى الميّه و هو كبير فكيف هو توزع فى الماء هي؟
+
+214
+00:15:40,240 --> 00:15:43,240
+هل هو دائب فى الماء؟ بيبين كأنه colloidal
+
+215
+00:15:43,240 --> 00:15:47,460
+suspension كأنه محلول غروي أو محلول معلق فى الماء
+
+216
+00:15:47,460 --> 00:15:51,340
+لأنه بيسمح بوجود الـ structure that allow large
+
+217
+00:15:51,340 --> 00:15:54,520
+molecules to remain colloidally suspended in water
+
+218
+00:15:54,520 --> 00:15:58,300
+هذا المركب اللي بيكون معلق فى الماء our term
+
+219
+00:15:58,300 --> 00:16:02,940
+بنسميه الـ micelles الـ micelles هو المركب المعلق فى
+
+220
+00:16:02,940 --> 00:16:08,760
+الماء طيب، دي حالة الكازينة بيحكي ليه؟ اللي هو 
+
+221
+00:16:08,760 --> 00:16:12,500
+الـ micelles اللي بتتكونها الكازين اللي بتلت انواع من
+
+222
+00:16:12,500 --> 00:16:16,160
+البروتينات بيكون للخارج the part of the casein
+
+223
+00:16:16,160 --> 00:16:19,760
+molecule that have an affinity for water from the
+
+224
+00:16:19,760 --> 00:16:24,020
+outside of casein micelle اللي بيكون محبب للماء،
+
+225
+00:16:24,020 --> 00:16:25,200
+وعنده جاذبية للماء
+
+226
+00:16:30,120 --> 00:16:33,700
+في خارج الـ micelles ومن هو هنا في حالة الـ casein
+
+227
+00:16:33,700 --> 00:16:38,120
+كل الخارج هو الـ kappa-casein على العكس ال part of
+
+228
+00:16:38,120 --> 00:16:40,720
+casein molecules that are hidden ده هو طرف اللي
+
+229
+00:16:40,720 --> 00:16:44,140
+بيكره الميه اللي بيحبش الميه، بيذوبش في الميه،
+
+230
+00:16:44,140 --> 00:16:47,740
+form the inner core of the micelles، بيكون في
+
+231
+00:16:47,740 --> 00:16:51,480
+الداخل، في الجزء الداخلي ومن هم اللي بيكره الميه
+
+232
+00:16:51,480 --> 00:16:56,980
+كل الداخل هي الـ α و الـ β-casein بحكيلي الكزين
+
+233
+00:16:56,980 --> 00:17:00,300
+is present as Caseinates which mean that it's bind to
+
+234
+00:17:00,300 --> 00:17:04,340
+calcium and magnesium الكزين لما يترسب إذا صار
+
+235
+00:17:04,340 --> 00:17:08,840
+لترسيب بنسميه كازينات بتحول اللي عادي اسمها كازينات
+
+236
+00:17:08,840 --> 00:17:13,940
+يعني هو الكزين المترسب متى هاد هيترسب إذا قدر
+
+237
+00:17:13,940 --> 00:17:18,540
+يرتبط مع كالسيوم أو مغنيسيوم بتعمل ionization و
+
+238
+00:17:18,540 --> 00:17:20,060
+بترسب الكزين
+
+239
+00:17:23,090 --> 00:17:26,910
+طب هنا عندنا صور للألفا والبيتا والكابا كزين في
+
+240
+00:17:26,910 --> 00:17:30,570
+عندنا لليمين الصورة اللي تحت الأبيض بالأسم ده هي
+
+241
+00:17:30,570 --> 00:17:35,010
+عبارة عن صور ل ال micelles للكزين micelles اللي هو
+
+242
+00:17:35,010 --> 00:17:39,130
+الكابا والبيتا والألفا كزين مع بعض كيف شكل ال
+
+243
+00:17:39,130 --> 00:17:44,250
+micelles تحت المجهد ممكنها كرة كريات تحتوي من
+
+244
+00:17:44,250 --> 00:17:48,190
+الكابا من الخارج وبيتا وألفا في الداخل
+
+245
+00:17:50,410 --> 00:17:55,170
+هنا صورة الـ Micelles الكورة هذه الكتلة تتكوين من
+
+246
+00:17:55,170 --> 00:17:58,950
+α و β و κ α أو β للداخل و κ
+
+247
+00:17:58,950 --> 00:18:04,130
+للخارج هي مكبرة بالإلكترون مايكروسكوبي تحت المجهر
+
+248
+00:18:04,130 --> 00:18:09,750
+الإلكتروني صورة من المجهر الإلكتروني حكينا في
+
+249
+00:18:09,750 --> 00:18:12,730
+المعمل السابق أن الـ isoelectric point لمعظم
+
+250
+00:18:12,730 --> 00:18:18,470
+البروتينات تتروح من pH 4 إلى 6 اللي بترا الـ γ كمان
+
+251
+00:18:18,470 --> 00:18:22,970
+من البروتينات هي من pH 4 إلى 6 طيب ليش ال كازين
+
+252
+00:18:22,970 --> 00:18:27,630
+دائب في الحليب؟ why casein is soluble in milk؟
+
+253
+00:18:27,630 --> 00:18:31,650
+هلجد؟ هو هيتوب حكينا بيكون دائب إذا ال pH أعلى من
+
+254
+00:18:31,650 --> 00:18:36,230
+ال PI أو أقل من ال PI لكن متى فاتساوة ال pH مع ال
+
+255
+00:18:36,230 --> 00:18:42,690
+PI يترسب؟ هلجد؟ الحليب بيحكيني أنه ليش هو دائب
+
+256
+00:18:42,690 --> 00:18:50,050
+لأنه ال PI للـ pH للحليب هي 6.6 الـ PI
+
+257
+00:18:50,050 --> 00:18:55,590
+للكازين 4.7 الـ pH للحليب هي القاعدي 6.6 فالـ
+
+258
+00:18:55,590 --> 00:19:00,570
+pH للحليب أعلى من الـ PI مش متساوية، هي أعلى، بما
+
+259
+00:19:00,570 --> 00:19:01,290
+أنها أعلى
+
+260
+00:19:04,750 --> 00:19:08,190
+بتكون الشحنات نيجاتيف اللي على الكزيد، pH أعلى من
+
+261
+00:19:08,190 --> 00:19:14,830
+الـ PI، شحنات متشابه حاجانة بتتنافر، بيصير قوة
+
+262
+00:19:14,830 --> 00:19:17,450
+تنافر، بتتوزع فيه السائل و بتكون
+
+263
+00:19:22,570 --> 00:19:27,230
+بالانمال فكيف بدي أداوبها لازم أوصل الـ pH تاعت
+
+264
+00:19:27,230 --> 00:19:32,150
+الحنيب للـ PI للكازين ممكن هادي أعملها عن طريق
+
+265
+00:19:32,150 --> 00:19:35,110
+ال bacterial action بفعل البكتيريا وحكينا في
+
+266
+00:19:35,110 --> 00:19:36,010
+المعمل السابق
+
+267
+00:19:39,810 --> 00:19:44,030
+بتجي بكتيريا تحول سكر اللاكتوز اللي موجود في
+
+268
+00:19:44,030 --> 00:19:48,530
+الحليب إلى lactic acid اللاكتك أسد هو عبارة عن حمض
+
+269
+00:19:48,530 --> 00:19:52,850
+والحمض هذا بيقلل ال pH فهيقلل ال pH بتاعة الملك من
+
+270
+00:19:52,850 --> 00:19:57,870
+6.6 اللي هو الطبيعي إلى 4.7 ال pH بتاعة الحليب
+
+271
+00:19:57,870 --> 00:20:04,450
+هيتقل من 6.6 إلى 4.7 4.7 صارت ال pH للحليب هي 4.7
+
+272
+00:20:04,450 --> 00:20:08,470
+طب و ال isoelectric point بتاعة الكازين 4.7
+
+273
+00:20:08,940 --> 00:20:13,100
+فصارت الـ pH للحليب مساوية للـ PI تاعت الكازين
+
+274
+00:20:13,100 --> 00:20:17,500
+وبالتالي بيترسب لأن حكينا ما تمت ساوية الـ pH مع
+
+275
+00:20:17,500 --> 00:20:20,160
+الـ PI، لذا بيترسب
+
+276
+00:20:24,520 --> 00:20:29,420
+طرق ترسيب الكازين كيف ممكن أعمل precipitation of
+
+277
+00:20:29,420 --> 00:20:33,520
+casein؟ أول طريقة ممكن بإضافة excess calcium أيون،
+
+278
+00:20:33,520 --> 00:20:37,760
+إنه إضافة كميات كبيرة من أيونات الكالسيوم بحيث
+
+279
+00:20:37,760 --> 00:20:41,520
+إنها توصل للـ α و الـ β-casein وتأين
+
+280
+00:20:41,520 --> 00:20:46,400
+الفسفات جروب اللي موجودة فيها ويترسب يصير Intolerant
+
+281
+00:20:46,400 --> 00:20:50,640
+طريقة تانية ممكن بإضافة acid أنه أضيف حمض زي ال
+
+282
+00:20:50,640 --> 00:20:55,280
+HCl أو ال Acetic acid لأن وسط الحليب حكينا أنه وسط
+
+283
+00:20:55,280 --> 00:21:00,520
+قاعدي بيكون alkaline 6.6 ال pH للملك و ال PI
+
+284
+00:21:00,520 --> 00:21:06,720
+للكازين هي 4.7 فانا بدي أوصل ال pH بتاع الملك من 6
+
+285
+00:21:06,720 --> 00:21:11,400
+و 6 إلى 4 و 7 كيف بدي أجللها أنه أضيف حمض الحمض
+
+286
+00:21:11,400 --> 00:21:17,290
+هيقلل ال pH فتوصل ال pH للـ PI للـ casein
+
+287
+00:21:17,290 --> 00:21:21,490
+ويترسب الـ casein هذا مبدأ عملنا لليوم إن أنا بدأ
+
+288
+00:21:21,490 --> 00:21:26,150
+أضيف حمض عشان يقلل ال pH و أرسب ال casein
+
+289
+00:21:26,150 --> 00:21:29,330
+طريقة التالتة ممكن بضعفة الـ Renin إنزيم
+
+290
+00:21:29,330 --> 00:21:33,390
+طريقة الرابعة بضعفة عن طريقة الـ Bacterial action
+
+291
+00:21:33,390 --> 00:21:37,010
+اللي حكينا عنه إنه كيف تشتغل البكتيريا وتحويل سكر
+
+292
+00:21:37,010 --> 00:21:41,190
+اللاكتوز إلى Lactic acid مدام و أسد بيقلل ال pH
+
+293
+00:21:41,190 --> 00:21:46,650
+لحليب بسبب الـ Renin enzyme كيف بيشتغل، كيف
+
+294
+00:21:46,650 --> 00:21:50,250
+بيرسل بالكاتين الـ Renin enzyme هو أنزيم باللاجئ
+
+295
+00:21:50,250 --> 00:21:54,270
+موجود فيه الـ calves و ال goats موجود فيه العجل و
+
+296
+00:21:54,270 --> 00:21:58,830
+العنزة أو الماعز، هو عبارة عن هيدروليسز إنزيم هو
+
+297
+00:21:58,830 --> 00:22:02,590
+إنزيم مكسر، بيطلق عليه peptidase، peptidase يعني
+
+298
+00:22:02,590 --> 00:22:06,410
+إنزيمات بتكسر، هو بيكسر الـ peptide bond بين
+
+299
+00:22:06,410 --> 00:22:12,710
+الأحماض الأمينية طب كيف يشتغل؟ كيف الميكانيزم للـ
+
+300
+00:22:12,710 --> 00:22:16,150
+Renin؟ الـ Renin هو إنزيم حكينا Hydrolysis
+
+301
+00:22:16,150 --> 00:22:19,950
+إنزيم، إنزيم مكسر، بيروح على الكابا-casein، على
+
+302
+00:22:19,950 --> 00:22:22,170
+الموجود اللي يمين للخارج، اللي بالظهر،
+
+303
+00:22:22,170 --> 00:22:25,330
+الكابا-casein، بيروح على الـ Renin، الكابا-casein
+
+304
+00:22:25,330 --> 00:22:28,470
+بيعمل عليها تكسيرها، بيعمل Hydrolysis breaking the
+
+305
+00:22:28,470 --> 00:22:33,250
+peptide bond، بيحولها لحاجة بسميها Para-kappa-casein
+
+306
+00:22:34,590 --> 00:22:38,790
+هو كان عامل حماية كجدار حماية للـ β و الـ α
+
+307
+00:22:38,790 --> 00:22:42,810
+اللي في داخله لما يتكسر ويتحول لـ Para-kappa-casein
+
+308
+00:22:42,810 --> 00:22:46,530
+اللي هو عبارة عن كازين مكسر فيه فتحات يعني صارت
+
+309
+00:22:46,530 --> 00:22:51,310
+α و β ظاهرين للخارج فهو بيقدرش يحميهم،
+
+310
+00:22:51,310 --> 00:22:55,770
+Cannot prevent casein from precipitate فالبرا كابا
+
+311
+00:22:55,770 --> 00:22:58,430
+كازين هذا ما عادوش قدرة أنه يحمي الكازين من الترسب
+
+312
+00:22:58,430 --> 00:23:02,910
+بليش؟ لأنه Para-kappa-casein مكسر لدهرة الألفا
+
+313
+00:23:02,910 --> 00:23:06,530
+والبيتا لوجود الكالسيوم اللي موجود في الحليب بيقدر
+
+314
+00:23:06,530 --> 00:23:11,350
+يدخل لجوا ويعمل ionization للألفا والبيتا كازين
+
+315
+00:23:11,350 --> 00:23:16,070
+لمجموعات الفسبات الموجودة فيه مدام اتأينه خلاص
+
+316
+00:23:16,070 --> 00:23:19,910
+بيصير ال precipitate بيترسب بحكي ليه لو الـ Calcium
+
+317
+00:23:19,910 --> 00:23:23,070
+removed from milk، the renin cannot form a
+
+318
+00:23:23,070 --> 00:23:26,390
+precipitate to milk. إذا الـ Calcium شيلته أنا من
+
+319
+00:23:26,390 --> 00:23:30,190
+الحليب، وكسر .. واجه الـ renin انزعي، كسر الكابا
+
+320
+00:23:30,190 --> 00:23:33,230
+كازين، خلاص، مافيش Calcium، مش هيدخل على الـ α
+
+321
+00:23:33,230 --> 00:23:36,650
+والـ β، ومش هيعمل الـ precipitation. لكن الـ
+
+322
+00:23:36,650 --> 00:23:40,050
+renin لازم مين يشتغل معاه؟ الـ renin يكسر ويجي الـ
+
+323
+00:23:40,050 --> 00:23:43,470
+Calcium، يعمل Ionization، عشان يعمل ال
+
+324
+00:23:43,470 --> 00:23:48,430
+precipitation لكازين. طب البكتيريا الـ Action إيه
+
+325
+00:23:48,430 --> 00:23:52,290
+عادة اللي ما حكيناها؟ البكتيريا اللي حكيناها إنها
+
+326
+00:23:52,290 --> 00:23:56,430
+بتيجي على الحليب، بتعمل على هضم اللاكتوز، بتكون
+
+327
+00:23:56,430 --> 00:23:59,910
+منه lactic acid، بتحول سكر اللاكتوز اللي موجود في
+
+328
+00:23:59,910 --> 00:24:04,340
+الحليب إلى lactic acid Lactic acid هذا حمض فبقلل
+
+329
+00:24:04,340 --> 00:24:10,260
+الـ pH للحليب من 6.6 إلى 4.7 تصير الـ pH للحليب 4
+
+330
+00:24:10,260 --> 00:24:14,240
+.7 4.7 هي نفسها الـ isoelectric point بتاعة
+
+331
+00:24:14,240 --> 00:24:19,240
+الكازين بدل من الـ pH تساوي الـ PI إذا بيترسب
+
+332
+00:24:19,240 --> 00:24:25,050
+الكازين اللي إذا جلت البيئة و البيئة ليه في ال
+
+333
+00:24:25,050 --> 00:24:28,530
+degrees of milk جلت البيئة تحت الحليب ووصلت إلى ال
+
+334
+00:24:28,530 --> 00:24:32,170
+isoelectric point بتاعت الكازين صاروا متساوين
+
+335
+00:24:32,170 --> 00:24:36,270
+بينفصل الحليب إلى طبقتين حاجة بتسميها Curds and
+
+336
+00:24:36,270 --> 00:24:40,600
+Whey بينفصل الحليب إلى Curds and Whey الـ Curds هي
+
+337
+00:24:40,600 --> 00:24:43,160
+عبارة عن الكازين هو اللي بيكون بالأسفل، الـ
+
+338
+00:24:43,160 --> 00:24:45,500
+precipitate اللي بينزل للأسفل اللي هو بروتين
+
+339
+00:24:45,500 --> 00:24:48,560
+الكازين اللي هو الـ dairy product obtained by
+
+340
+00:24:48,560 --> 00:24:51,440
+precipitation of milk with renin or acidic
+
+341
+00:24:51,440 --> 00:24:56,400
+substance and then the draining of the liquid
+
+342
+00:24:56,400 --> 00:25:00,610
+portion الـ Curds هاد عبارة عن المنتج اليومي اللي
+
+343
+00:25:00,610 --> 00:25:04,030
+بينتج من تلصيب الحليب باستخدام سواء الـ renin
+
+344
+00:25:04,030 --> 00:25:09,050
+enzyme أو باستخدام مادة حمضية فتنزل لتحت هذا
+
+345
+00:25:09,050 --> 00:25:12,110
+البريس الكيردس و تكون فيه liquid portion في الأعلى
+
+346
+00:25:12,110 --> 00:25:16,110
+ال liquid portion هذا اللي في الأعلى بيسوي whey أو
+
+347
+00:25:16,110 --> 00:25:19,930
+milk serum is the liquid remaining after milk has
+
+348
+00:25:19,930 --> 00:25:23,670
+been coagulated and removal of curds الحليب إذا
+
+349
+00:25:23,670 --> 00:25:27,730
+قللت ال pH ووصلتها ل ال PI تاعت الكازين ينفصل إلى
+
+350
+00:25:27,730 --> 00:25:31,250
+طبقتين حاجة بتسميها Curds بتكون في الأسفل اللي هو
+
+351
+00:25:31,250 --> 00:25:34,690
+الطبقة السميكة اللي بتكون في الأسفل اللي هي المنتج
+
+352
+00:25:34,690 --> 00:25:38,550
+اليومي اللي هو عبارة عن الكازين في الأعلى بيكون ال
+
+353
+00:25:38,550 --> 00:25:41,610
+whey أو الملك سيروم اللي هو الجزء السائل اللي هو
+
+354
+00:25:41,610 --> 00:25:43,130
+باقي المكونات
+
+355
+00:25:45,120 --> 00:25:49,760
+طيب إيش هو معملنا لليوم؟ معملنا لليوم هو عبارة عن
+
+356
+00:25:49,760 --> 00:25:54,260
+إيش هو يعني ال objective منه إنه isolate casein
+
+357
+00:25:54,260 --> 00:25:57,980
+from milk and calculate a percentage إن أنا بتعزل
+
+358
+00:25:57,980 --> 00:26:01,960
+ال casein من الحليب وود أحسبله قداش النسبة
+
+359
+00:26:01,960 --> 00:26:04,600
+المقاوية اللي هو ال percentage، قداش نسبة موجودة
+
+360
+00:26:04,600 --> 00:26:08,460
+في الحليب بنان على أي مبتدأ الـ principle استخدام
+
+361
+00:26:08,460 --> 00:26:11,260
+Acetic Acid اللي أنا بتستخدم حمض هو الـ Acetic
+
+362
+00:26:11,260 --> 00:26:16,440
+Acid أضيفه على الكازين يعطيني Caseinates اللي هو
+
+363
+00:26:16,440 --> 00:26:20,980
+ترسيب للكازين، لذا هو المبتدأ ان الـ principle أن
+
+364
+00:26:20,980 --> 00:26:27,450
+أنا أضيف Acetic Acid زي كزين يعطيني كزينة طيب كيف
+
+365
+00:26:27,450 --> 00:26:32,450
+الخطوات؟ بدي أجيب cup أحط عليه فيه عشرة مل من
+
+366
+00:26:32,450 --> 00:26:37,950
+الحليب و أحطه في ال water bath على أربعين درجة
+
+367
+00:26:37,950 --> 00:26:41,710
+مئوية لخمسة minutes أحطها على درجة حرارة في
+
+368
+00:26:41,710 --> 00:26:45,370
+الميه على درجة حرارة عارفين درجة لمدة خمس دقائق
+
+369
+00:26:45,370 --> 00:26:51,160
+هذا بس عشان أدفئ الحليب بعدين هطلعه و هضيف عليه 15
+
+370
+00:26:51,160 --> 00:26:55,460
+drop من ال Acetic Acid هضيف عليه Acetic Acid حمض
+
+371
+00:26:55,460 --> 00:26:59,240
+عشان يقلل البيئة عشان هيبقى على 15 drop Acetic
+
+372
+00:26:59,240 --> 00:27:03,720
+Acid لتقليل البيئة و أعمل mix هلاجي الحليب فصل
+
+373
+00:27:03,720 --> 00:27:08,320
+عندي لطبقتين بيكون كل المكونات فوق و تحت بينزل
+
+374
+00:27:
+
+401
+00:28:54,920 --> 00:28:58,880
+حطيتها اللي اجت فيها بتطير فبيضل بس عليها الكازين
+
+402
+00:28:58,880 --> 00:29:03,720
+بعد ما تجف بوزلها في الميزان بحطها وبسجل عندي 
+
+403
+00:29:03,720 --> 00:29:06,200
+الوزنة كتاش الوزنة عندي
+
+404
+00:29:09,850 --> 00:29:12,190
+يعني الوزن اللي أنا طلعتها بتحسب منها ال
+
+405
+00:29:12,190 --> 00:29:16,050
+percentage of casein in milk قداش نسبة الـ casein
+
+406
+00:29:16,050 --> 00:29:19,450
+الموجود في الحليب الـ percentage of casein بتساوي
+
+407
+00:29:19,450 --> 00:29:22,530
+الـ gram of casein قداش أنا وزنت الـ casein اللي
+
+408
+00:29:22,530 --> 00:29:27,030
+طلعتها على الـ gram of milk وزنة الحليب درمية في
+
+409
+00:29:27,030 --> 00:29:30,220
+المية الـ gram of casein هذه الوزن اللي أنا وزنتها
+
+410
+00:29:30,220 --> 00:29:32,520
+في الآخر اللي كانت على الورقة ووزنتها على الميزان
+
+411
+00:29:32,520 --> 00:29:36,240
+جديش طلعت ماني الـ gram of milk اللي هي الحليب جديش
+
+412
+00:29:36,240 --> 00:29:39,540
+أنا أخد كمية أول شي عشرة ml من الحليب فهو عشرة
+
+413
+00:29:39,540 --> 00:29:44,020
+ضرب مية في المية وبطلع النسبة وبالـ normal range
+
+414
+00:29:44,020 --> 00:29:49,160
+المفروض تطلع معنا نسبة الـ casein من تلاتة إلى خمسة
+
+415
+00:29:49,160 --> 00:29:52,000
+في المية تلاتة إلى خمسة في المية 
diff --git a/PL9fwy3NUQKwZ1Q7zOg1ObgufPMjRI3WY1/nTcz4I3nh3A_raw.json b/PL9fwy3NUQKwZ1Q7zOg1ObgufPMjRI3WY1/nTcz4I3nh3A_raw.json
new file mode 100644
index 0000000000000000000000000000000000000000..2c4a3b69faab210b53efea5d401c6c133d40a88a
--- /dev/null
+++ b/PL9fwy3NUQKwZ1Q7zOg1ObgufPMjRI3WY1/nTcz4I3nh3A_raw.json
@@ -0,0 +1 @@
+{"segments": [{"id": 1, "seek": 3041, "start": 1.89, "end": 30.41, "text": "اليوم راح نحكي بمعمل هو isolation of casein from milk اللي هو عزل ال protein ال casein من الحليب وهذا يعتبر تطبيق على المعمل الماضي إن أحنا صارنا عارفين ال isoelectric point لل casein بدنا نوصل البيت لحليب لل isoelectric point بتبعت ال casein وبالتالي بيترسب ال protein ال casein و تبقى باقي ال proteinات", "tokens": [6027, 1829, 20498, 12602, 39319, 8717, 5016, 4117, 1829, 4724, 2304, 25957, 1211, 31439, 16001, 295, 1389, 259, 490, 5392, 13672, 1829, 31439, 6225, 11622, 1211, 2423, 7944, 2423, 1389, 259, 9154, 21542, 20292, 3555, 37037, 15730, 7251, 34268, 26890, 6055, 9566, 21292, 4587, 15844, 9673, 25957, 1211, 9673, 46958, 1829, 36145, 5551, 5016, 8315, 20328, 9640, 8315, 6225, 9640, 5172, 9957, 2423, 307, 7921, 38421, 935, 24976, 1389, 259, 47525, 8315, 8717, 2407, 36520, 2423, 21292, 2655, 5296, 5016, 20292, 3555, 24976, 307, 7921, 38421, 935, 39894, 3555, 34268, 2423, 1389, 259, 46599, 6027, 2655, 6027, 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"probability": 0.861328125}, {"start": 19.35, "end": 19.71, "word": " نوصل", "probability": 0.8636067708333334}, {"start": 19.71, "end": 20.19, "word": " البيت", "probability": 0.5890299479166666}, {"start": 20.19, "end": 21.75, "word": " لحليب", "probability": 0.68853759765625}, {"start": 21.75, "end": 22.03, "word": " لل", "probability": 0.7197265625}, {"start": 22.03, "end": 22.61, "word": " isoelectric", "probability": 0.9111328125}, {"start": 22.61, "end": 23.05, "word": " point", "probability": 0.98876953125}, {"start": 23.05, "end": 23.37, "word": " بتبعت", "probability": 0.6220703125}, {"start": 23.37, "end": 23.57, "word": " ال", "probability": 0.8779296875}, {"start": 23.57, "end": 24.15, "word": " casein", "probability": 0.983154296875}, {"start": 24.15, "end": 25.33, "word": " وبالتالي", "probability": 0.89833984375}, {"start": 25.33, "end": 26.31, "word": " بيترسب", "probability": 0.852783203125}, {"start": 26.31, "end": 26.85, "word": " ال", "probability": 0.94384765625}, {"start": 26.85, "end": 27.41, "word": " protein", "probability": 0.95556640625}, {"start": 27.41, "end": 27.67, "word": " ال", "probability": 0.88671875}, {"start": 27.67, "end": 28.21, "word": " casein", "probability": 0.981689453125}, {"start": 28.21, "end": 28.39, "word": " و", "probability": 0.80859375}, {"start": 28.39, "end": 28.77, "word": " تبقى", "probability": 0.83349609375}, {"start": 28.77, "end": 29.35, "word": " باقي", "probability": 0.9479166666666666}, {"start": 29.35, "end": 29.57, "word": " ال", "probability": 0.94384765625}, {"start": 29.57, "end": 30.41, "word": " proteinات", "probability": 0.67333984375}], "temperature": 1.0}, {"id": 2, "seek": 5467, "start": 33.33, "end": 54.67, "text": "في البداية بدنا نحكي عن البروتين أنه ينقسم إلى قسمين نوع البروتين في simple protein و في conjugated protein بروتينات بسيطة أو بروتينات معقدة simple protein هي عبارة عن حماض أمينية مرتبطة مع بعضها البعض بروابط بيبتديا", "tokens": [41185, 29739, 28259, 10632, 47525, 8315, 8717, 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0.9814453125}, {"start": 34.89, "end": 35.19, "word": " عن", "probability": 0.9853515625}, {"start": 35.19, "end": 36.13, "word": " البروتين", "probability": 0.834228515625}, {"start": 36.13, "end": 36.89, "word": " أنه", "probability": 0.5291748046875}, {"start": 36.89, "end": 37.33, "word": " ينقسم", "probability": 0.894775390625}, {"start": 37.33, "end": 37.51, "word": " إلى", "probability": 0.9658203125}, {"start": 37.51, "end": 38.03, "word": " قسمين", "probability": 0.9851888020833334}, {"start": 38.03, "end": 38.35, "word": " نوع", "probability": 0.529205322265625}, {"start": 38.35, "end": 39.01, "word": " البروتين", "probability": 0.9736328125}, {"start": 39.01, "end": 39.53, "word": " في", "probability": 0.45556640625}, {"start": 39.53, "end": 39.85, "word": " simple", "probability": 0.45263671875}, {"start": 39.85, "end": 40.49, "word": " protein", "probability": 0.70556640625}, {"start": 40.49, "end": 40.69, "word": " و", "probability": 0.9501953125}, {"start": 40.69, "end": 40.83, "word": " في", "probability": 0.69873046875}, {"start": 40.83, "end": 41.81, "word": " conjugated", "probability": 0.960205078125}, {"start": 41.81, "end": 42.37, "word": " protein", "probability": 0.921875}, {"start": 42.37, "end": 43.49, "word": " بروتينات", "probability": 0.82998046875}, {"start": 43.49, "end": 44.19, "word": " بسيطة", "probability": 0.99248046875}, {"start": 44.19, "end": 44.83, "word": " أو", "probability": 0.9140625}, {"start": 44.83, "end": 45.53, "word": " بروتينات", "probability": 0.9396484375}, {"start": 45.53, "end": 46.31, "word": " معقدة", "probability": 0.9860026041666666}, {"start": 46.31, "end": 47.53, "word": " simple", "probability": 0.37158203125}, {"start": 47.53, "end": 48.21, "word": " protein", "probability": 0.91943359375}, {"start": 48.21, "end": 48.47, "word": " هي", "probability": 0.87158203125}, {"start": 48.47, "end": 48.99, "word": " عبارة", "probability": 0.9954833984375}, {"start": 48.99, "end": 49.47, "word": " عن", "probability": 0.99267578125}, {"start": 49.47, "end": 51.87, "word": " حماض", "probability": 0.6085611979166666}, {"start": 51.87, "end": 52.33, "word": " أمينية", "probability": 0.9156494140625}, {"start": 52.33, "end": 52.83, "word": " مرتبطة", "probability": 0.98193359375}, {"start": 52.83, "end": 52.97, "word": " مع", "probability": 0.982421875}, {"start": 52.97, "end": 53.29, "word": " بعضها", "probability": 0.89404296875}, {"start": 53.29, "end": 53.57, "word": " البعض", "probability": 0.98388671875}, {"start": 53.57, "end": 54.05, "word": " بروابط", "probability": 0.90146484375}, {"start": 54.05, "end": 54.67, "word": " بيبتديا", "probability": 0.79541015625}], "temperature": 1.0}, {"id": 3, "seek": 6737, "start": 55.67, "end": 67.37, "text": "يعني لو كسرتها هيدرولايزed to yield only amino acid زي الألبومين والجلوبيلين لو كسرتها بس منتج منها أحماط أمينية مثال عليها الألبومين والجلوبيلين", "tokens": [40228, 22653, 45164, 9122, 3794, 43500, 11296, 8032, 25708, 2288, 12610, 47302, 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"probability": 0.72412109375}, {"start": 58.83, "end": 59.41, "word": " amino", "probability": 0.791015625}, {"start": 59.41, "end": 60.07, "word": " acid", "probability": 0.52978515625}, {"start": 60.07, "end": 60.35, "word": " زي", "probability": 0.846923828125}, {"start": 60.35, "end": 61.03, "word": " الألبومين", "probability": 0.7474365234375}, {"start": 61.03, "end": 61.95, "word": " والجلوبيلين", "probability": 0.7388392857142857}, {"start": 61.95, "end": 62.13, "word": " لو", "probability": 0.6826171875}, {"start": 62.13, "end": 62.67, "word": " كسرتها", "probability": 0.9906005859375}, {"start": 62.67, "end": 62.89, "word": " بس", "probability": 0.943359375}, {"start": 62.89, "end": 63.33, "word": " منتج", "probability": 0.7696940104166666}, {"start": 63.33, "end": 63.61, "word": " منها", "probability": 0.981201171875}, {"start": 63.61, "end": 64.09, "word": " أحماط", "probability": 0.7023518880208334}, {"start": 64.09, "end": 64.69, "word": " أمينية", "probability": 0.8935546875}, {"start": 64.69, "end": 65.49, "word": " مثال", "probability": 0.6708984375}, {"start": 65.49, "end": 65.85, "word": " عليها", "probability": 0.983642578125}, {"start": 65.85, "end": 66.53, "word": " الألبومين", "probability": 0.9901123046875}, {"start": 66.53, "end": 67.37, "word": " والجلوبيلين", "probability": 0.9132254464285714}], "temperature": 1.0}, {"id": 4, "seek": 9514, "start": 68.06, "end": 95.14, "text": "conjugated protein بروتينات معقدة اللي بتحتوي على أحماض أمنية ومتصل فيها another group other chemical group بروتين that function in interaction with other chemical group attached by covalent bond or by weak interaction المتصل مع الأحماض الأمنية مجموعة تانية مجموعة group تانية ممكن تكون هذه المجموعة في صفية group ممكن تكون carbohydrate ممكن", "tokens": [1671, 42068, 770, 7944, 4724, 32887, 31371, 8315, 2655, 20449, 28543, 3660, 13672, 1829, 39894, 33753, 45865, 15844, 5551, 35571, 46958, 5551, 27842, 10632, 4032, 2304, 2655, 36520, 8978, 11296, 1071, 1594, 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"word": " protein", "probability": 0.89892578125}, {"start": 126.69, "end": 127.47, "word": " إذا", "probability": 0.912353515625}, {"start": 127.47, "end": 127.67, "word": " كان", "probability": 0.97412109375}, {"start": 127.67, "end": 127.89, "word": " بس", "probability": 0.811767578125}, {"start": 127.89, "end": 128.29, "word": " أحماض", "probability": 0.97705078125}, {"start": 128.29, "end": 128.97, "word": " أمينية", "probability": 0.9892578125}, {"start": 128.97, "end": 130.23, "word": " متصلة", "probability": 0.7803955078125}, {"start": 130.23, "end": 130.33, "word": " مع", "probability": 0.99072265625}, {"start": 130.33, "end": 130.57, "word": " بعض", "probability": 0.996826171875}, {"start": 130.57, "end": 130.89, "word": " البعض", "probability": 0.9396158854166666}, {"start": 130.89, "end": 131.37, "word": " بسميه", "probability": 0.7286376953125}, {"start": 131.37, "end": 131.81, "word": " simple", "probability": 0.363037109375}, {"start": 131.81, "end": 132.51, "word": " protein", "probability": 0.90625}], "temperature": 1.0}, {"id": 7, "seek": 15469, "start": 135.4, "end": 154.7, "text": "ممكن نحكي عن الـ Phosphoprotein حكينا إن هو عبارة عن بروتين أو عن أحماض أمينيا bonded to a substance containing phosphoric acid متصلة فيه مجموعة phosphate group أو أي مركّب مادة تحتوي على phosphoric acid إذا كان موجود مجموعة phosphate بسميه phosphoprotein", "tokens": [2304, 43020, 8717, 5016, 4117, 1829, 18871, 2423, 39184, 2623, 16378, 404, 81, 1370, 259, 11331, 4117, 1829, 8315, 36145, 31439, 6225, 3555, 9640, 3660, 18871, 4724, 32887, 2655, 9957, 34051, 18871, 5551, 35571, 46958, 5551, 2304, 9957, 25528, 41194, 281, 257, 12961, 19273, 19775, 16345, 8258, 44650, 36520, 3660, 8978, 3224, 3714, 7435, 2304, 2407, 27884, 46542, 1594, 34051, 36632, 3714, 31747, 11703, 3555, 3714, 18513, 3660, 6055, 33753, 45865, 15844, 19775, 16345, 8258, 11933, 15730, 25961, 3714, 29245, 23328, 3714, 7435, 2304, 2407, 27884, 903, 329, 950, 473, 4724, 38251, 1829, 3224, 19775, 404, 81, 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{"start": 456.92, "end": 457.7, "word": " بيختلفوا", "probability": 0.90087890625}, {"start": 457.7, "end": 457.94, "word": " في", "probability": 0.98583984375}, {"start": 457.94, "end": 458.54, "word": " الـ", "probability": 0.76416015625}, {"start": 458.54, "end": 458.84, "word": " molecular", "probability": 0.53125}, {"start": 458.84, "end": 459.52, "word": " weight", "probability": 0.93017578125}, {"start": 459.52, "end": 460.92, "word": " الوزن", "probability": 0.9757080078125}, {"start": 460.92, "end": 461.6, "word": " الجزائي", "probability": 0.879638671875}, {"start": 461.6, "end": 462.52, "word": " بتختلف", "probability": 0.8111165364583334}, {"start": 462.52, "end": 462.68, "word": " في", "probability": 0.97802734375}, {"start": 462.68, "end": 463.3, "word": " كمية", "probability": 0.99560546875}, {"start": 463.3, "end": 463.6, "word": " الـ", "probability": 0.8427734375}, {"start": 463.6, "end": 464.0, "word": " phosphorus", "probability": 0.352783203125}, {"start": 464.0, 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هدول التلاتة كمان اللي هم different hydrophobic and hydrophilic regions بختلف اللي هو", "tokens": [3555, 9957, 16095, 12399, 13672, 1829, 39896, 9122, 2304, 10632, 2423, 39184, 2623, 16378, 473, 1594, 4032, 2423, 39184, 46914, 17792, 3364, 11933, 1829, 8592, 8032, 2304, 16712, 1211, 9307, 3660, 2423, 26890, 35473, 9957, 9307, 22807, 8032, 2304, 20588, 12, 34, 651, 259, 4032, 33286, 12, 34, 651, 259, 4032, 591, 25637, 12, 34, 651, 259, 6055, 1211, 9307, 3660, 12399, 20588, 12, 34, 651, 259, 4032, 33286, 12, 34, 651, 259, 4032, 591, 25637, 12, 34, 651, 259, 4724, 5016, 4117, 1829, 20292, 8032, 3215, 12610, 16712, 1211, 9307, 3660, 9122, 2304, 7649, 13672, 1829, 8032, 2304, 819, 5796, 11741, 22234, 293, 5796, 11741, 388, 299, 10682, 4724, 46456, 46538, 13672, 1829, 31439], "avg_logprob": -0.2570043170246585, "compression_ratio": 1.7547169811320755, "no_speech_prob": 0.00013375282287597656, "words": [{"start": 471.16, "end": 472.12, "word": "بينهم،", "probability": 0.536407470703125}, 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ال major casein هو الأكبر أعلى molecular weight أعلى من بيتا و أعلى من كعبة بيتكون من تمانية إلى عشرة فسفات جروم ال Alpha-Casein هو أكتر", "tokens": [41185, 9154, 11296, 9122, 9640, 3224, 5296, 19528, 16606, 12399, 8978, 9154, 11296, 3714, 5016, 3555, 5296, 19528, 16606, 12399, 8978, 9154, 11296, 4724, 1829, 12984, 38688, 8978, 9673, 16606, 12399, 4032, 41185, 20193, 8717, 1829, 7435, 1829, 8717, 5016, 4117, 1829, 18871, 16712, 1211, 9307, 2423, 26890, 35473, 9957, 9307, 9673, 4587, 995, 20498, 3660, 24976, 4117, 31377, 9957, 20588, 12, 34, 651, 259, 31439, 2423, 2563, 1389, 259, 31439, 16247, 4117, 26890, 5551, 3615, 23942, 19046, 3364, 5551, 3615, 23942, 9154, 4724, 36081, 995, 4032, 5551, 3615, 23942, 9154, 9122, 3615, 49401, 4724, 36081, 30544, 9154, 46811, 7649, 10632, 30731, 6225, 8592, 25720, 6156, 3794, 5172, 9307, 10874, 32887, 2304, 2423, 20588, 12, 34, 651, 259, 31439, 5551, 4117, 2655, 2288], "avg_logprob": -0.2993951713846576, "compression_ratio": 1.8451882845188285, 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يحتوي فقط هو أقل Molecular weight و يحتوي فقط على 2 Phosphate Group بس على 2 Phosphate Group النسبة الأكبر فيه هي Carbohydrate هي التأول من Carbohydrate مليء بالسُكَر الكابا كازين مش زي Alpha و Beta كازين يحتوي فقط هو أقل", "tokens": [9566, 3555, 20666, 1863, 35457, 3660, 5296, 9122, 16758, 995, 9122, 31377, 9957, 33251, 16758, 995, 9122, 31377, 9957, 37893, 30767, 1829, 20588, 4032, 33286, 9122, 31377, 9957, 7251, 33753, 45865, 6156, 47432, 31439, 5551, 4587, 1211, 46914, 17792, 3364, 4032, 7251, 33753, 45865, 6156, 47432, 15844, 568, 2623, 16378, 473, 10500, 4724, 3794, 15844, 568, 2623, 16378, 473, 10500, 28239, 35457, 3660, 16247, 4117, 26890, 8978, 3224, 39896, 2741, 65, 1445, 6655, 4404, 39896, 16712, 10721, 12610, 9154, 2741, 65, 1445, 6655, 4404, 3714, 20292, 38207, 20666, 3794, 10859, 4117, 6808, 2288, 33251, 16758, 995, 9122, 31377, 9957, 37893, 30767, 1829, 20588, 4032, 33286, 9122, 31377, 9957, 7251, 33753, 45865, 6156, 47432, 31439, 5551, 4587, 1211], "avg_logprob": 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1141.29, "text": "والـ pH للحليب هي 6 و 6 الـ Bi للكازين 4 و 7 الـ pH للحليب هي القاعدي 6 و 6 فالـ pH للحليب أعلى من الـ Bi مش متساوية، هي أعلى، بما أنها أعلى", "tokens": [2407, 6027, 39184, 21677, 24976, 5016, 20292, 3555, 39896, 1386, 4032, 1386, 2423, 39184, 13007, 24976, 4117, 31377, 9957, 1017, 4032, 1614, 2423, 39184, 21677, 24976, 5016, 20292, 3555, 39896, 25062, 995, 22488, 1829, 1386, 4032, 1386, 6156, 6027, 39184, 21677, 24976, 5016, 20292, 3555, 5551, 3615, 23942, 9154, 2423, 39184, 13007, 37893, 44650, 3794, 995, 2407, 10632, 12399, 39896, 5551, 3615, 23942, 12399, 4724, 15042, 14739, 11296, 5551, 3615, 23942], "avg_logprob": -0.24262153026130465, "compression_ratio": 1.7829457364341086, "no_speech_prob": 0.002384185791015625, "words": [{"start": 1126.17, "end": 1126.59, "word": "والـ", "probability": 0.3908284505208333}, {"start": 1126.59, "end": 1126.85, "word": " pH", "probability": 0.6787109375}, {"start": 1126.85, "end": 1127.75, "word": " للحليب", "probability": 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{"start": 1132.35, "end": 1132.91, "word": " للحليب", "probability": 0.9840087890625}, {"start": 1132.91, "end": 1133.05, "word": " هي", "probability": 0.75927734375}, {"start": 1133.05, "end": 1133.71, "word": " القاعدي", "probability": 0.7369384765625}, {"start": 1133.71, "end": 1134.11, "word": " 6", "probability": 0.9833984375}, {"start": 1134.11, "end": 1134.47, "word": " و", "probability": 0.98046875}, {"start": 1134.47, "end": 1134.87, "word": " 6", "probability": 0.8798828125}, {"start": 1134.87, "end": 1135.59, "word": " فالـ", "probability": 0.9054361979166666}, {"start": 1135.59, "end": 1135.95, "word": " pH", "probability": 0.9755859375}, {"start": 1135.95, "end": 1136.77, "word": " للحليب", "probability": 0.9859619140625}, {"start": 1136.77, "end": 1137.19, "word": " أعلى", "probability": 0.9459635416666666}, {"start": 1137.19, "end": 1137.35, "word": " من", "probability": 0.994140625}, {"start": 1137.35, "end": 1137.57, "word": " الـ", "probability": 0.906982421875}, {"start": 1137.57, "end": 1137.89, "word": " Bi", "probability": 0.81787109375}, {"start": 1137.89, "end": 1138.27, "word": " مش", "probability": 0.7109375}, {"start": 1138.27, "end": 1139.07, "word": " متساوية،", "probability": 0.8585205078125}, {"start": 1139.07, "end": 1139.21, "word": " هي", "probability": 0.9404296875}, {"start": 1139.21, "end": 1140.35, "word": " أعلى،", "probability": 0.8837890625}, {"start": 1140.35, "end": 1140.57, "word": " بما", "probability": 0.6500244140625}, {"start": 1140.57, "end": 1140.87, "word": " أنها", "probability": 0.839599609375}, {"start": 1140.87, "end": 1141.29, "word": " أعلى", "probability": 0.9646809895833334}], "temperature": 1.0}, {"id": 48, "seek": 115745, "start": 1144.75, "end": 1157.45, "text": "بتكون الشحنات نيجاتيف اللي على الكزيد، pH أعلى من الـPI، شحنات متشابه حاجانة بتتنافر، بيصير قوة تنافر، بتتوزع فيه السائل و بتكون", "tokens": [3555, 2655, 30544, 25124, 5016, 8315, 2655, 8717, 1829, 7435, 9307, 33911, 13672, 1829, 15844, 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بدل من الـ pH تساوي الـ PI إذا بيترسم الكازين", "tokens": [43, 19892, 8258, 23758, 11331, 2304, 11242, 6156, 3555, 4587, 1211, 1211, 2423, 39184, 21677, 24976, 5016, 20292, 3555, 9154, 1386, 13, 21, 30731, 1017, 13, 22, 6055, 9381, 13546, 2423, 39184, 21677, 24976, 5016, 20292, 3555, 1017, 13, 22, 1017, 13, 22, 39896, 8717, 36178, 11296, 2423, 39184, 307, 7921, 38421, 935, 39894, 995, 27884, 33251, 31377, 9957, 47525, 1211, 9154, 2423, 39184, 21677, 6055, 3794, 995, 45865, 2423, 39184, 27176, 11933, 15730, 4724, 36081, 2288, 38251, 33251, 31377, 9957], "avg_logprob": -0.262576228598269, "compression_ratio": 1.4748603351955307, "no_speech_prob": 3.0994415283203125e-06, "words": [{"start": 1442.14, "end": 1442.6, "word": "Lactic", "probability": 0.5968017578125}, {"start": 1442.6, "end": 1442.92, "word": " acid", "probability": 0.7041015625}, {"start": 1442.92, "end": 1443.16, "word": " هذا", "probability": 0.66357421875}, {"start": 1443.16, "end": 1443.58, "word": " حمض", "probability": 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like dissolve like إن المضيبات بديبانه إيش المادة اللي بتجيبها ال lipid؟ زي ال ethanol أو ether ethanol فبدي أضيف على ال cup على ال casein و ال lipid ده أضيف خمسة من ال ethanol", "tokens": [45340, 29245, 23328, 4724, 47283, 3215, 16095, 9154, 2423, 13063, 7435, 11296, 11778, 12610, 4032, 4724, 5016, 9566, 16095, 8978, 4414, 4724, 5016, 9566, 16095, 8978, 4414, 5296, 7435, 3224, 3660, 2423, 4414, 23758, 20328, 28480, 1829, 3224, 1389, 259, 4032, 8280, 327, 41850, 4724, 16254, 4724, 3794, 2423, 1389, 259, 3714, 16758, 16254, 8592, 2423, 8280, 327, 9122, 33911, 4724, 16254, 5551, 2655, 9778, 1211, 9381, 9154, 2423, 8280, 327, 22807, 11778, 3224, 5551, 7435, 1829, 3555, 3714, 18513, 3660, 4724, 25708, 37746, 8978, 11296, 2423, 8280, 327, 15844, 3714, 44510, 10721, 411, 30150, 411, 36145, 9673, 11242, 1829, 3555, 9307, 4724, 16254, 3555, 7649, 3224, 11933, 1829, 8592, 9673, 18513, 3660, 13672, 1829, 39894, 7435, 1829, 3555, 11296, 2423, 8280, 327, 22807, 30767, 1829, 2423, 43150, 34051, 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new file mode 100644
index 0000000000000000000000000000000000000000..3b66668801ed4787617a3ef3a97f547992d8eb64
--- /dev/null
+++ b/PL9fwy3NUQKwZ1Q7zOg1ObgufPMjRI3WY1/pG0PJIJ68bY_raw.json
@@ -0,0 +1 @@
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some contain فسفرة استحتوي على فسفرة إذا كلها فيها كربون وهيدروجين وأكسجين ونيتروجين all ال protein", "tokens": [28820, 2423, 26890, 35473, 9957, 9307, 4724, 8592, 28820, 6225, 10943, 8978, 2423, 3877, 39894, 2655, 30544, 9154, 9122, 25513, 11536, 12399, 8032, 25708, 2288, 29245, 9957, 12399, 5551, 4117, 3794, 7435, 9957, 4032, 22653, 2655, 2288, 29245, 9957, 28242, 11296, 28242, 2423, 26890, 35473, 9957, 9307, 2423, 3877, 8978, 3224, 9122, 25513, 11536, 12399, 8032, 25708, 2288, 29245, 9957, 12399, 5551, 4117, 3794, 7435, 9957, 4032, 22653, 2655, 2288, 29245, 9957, 20449, 19913, 2304, 11296, 7251, 33753, 45865, 15844, 8608, 46538, 3660, 46599, 3615, 11242, 11296, 512, 5304, 6156, 3794, 5172, 25720, 44713, 33753, 45865, 15844, 6156, 3794, 5172, 25720, 11933, 15730, 28242, 11296, 8978, 11296, 9122, 25513, 11536, 4032, 3224, 25708, 2288, 29245, 9957, 4032, 10721, 4117, 3794, 7435, 9957, 4032, 22653, 2655, 2288, 29245, 9957, 439, 2423, 7944], "avg_logprob": -0.1730587071541584, 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"probability": 0.991455078125}], "temperature": 1.0}, {"id": 6, "seek": 14391, "start": 118.21, "end": 143.91, "text": "هنا عندنا توضيح كيف بيتكون البروتين هنا بيجي حمضيين حمض أميني بيرتبط مع حمض أميني آخر ب peptide bond وين بتتكون ال peptide bond؟ بين كربوكسيل من حمض أميني الأول و amino group من حمض أميني آخر بتيجي الرابطة بالنص بين الكربوكسيل و ال amino group بين الحمضيين الأمينية نسميها رابطة peptide bond", "tokens": [3224, 8315, 43242, 8315, 6055, 2407, 11242, 1829, 5016, 9122, 33911, 4724, 36081, 30544, 2423, 26890, 35473, 9957, 34105, 4724, 1829, 7435, 1829, 11331, 2304, 11242, 1829, 9957, 11331, 2304, 11242, 5551, 2304, 9957, 1829, 4724, 13546, 2655, 3555, 9566, 20449, 11331, 2304, 11242, 5551, 2304, 9957, 1829, 19753, 34740, 4724, 41781, 482, 6086, 4032, 9957, 39894, 2655, 30544, 2423, 41781, 482, 6086, 22807, 49374, 9122, 25513, 2407, 4117, 3794, 26895, 9154, 11331, 2304, 11242, 5551, 2304, 9957, 1829, 16247, 12610, 4032, 24674, 1594, 9154, 11331, 2304, 11242, 5551, 2304, 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{"start": 262.71, "end": 263.61, "word": " أثناء", "probability": 0.9580078125}, {"start": 263.61, "end": 263.75, "word": " ال", "probability": 0.9560546875}, {"start": 263.75, "end": 264.41, "word": " exercise", "probability": 0.8974609375}, {"start": 264.41, "end": 264.97, "word": " بيخزن", "probability": 0.98466796875}, {"start": 264.97, "end": 265.07, "word": " ال", "probability": 0.9248046875}, {"start": 265.07, "end": 265.39, "word": " oxygen", "probability": 0.876953125}, {"start": 265.39, "end": 265.57, "word": " في", "probability": 0.93701171875}, {"start": 265.57, "end": 265.97, "word": " العضلات", "probability": 0.804443359375}], "temperature": 1.0}, {"id": 12, "seek": 29301, "start": 266.39, "end": 293.01, "text": "إذا الجسم مثلا في أثناء التمرين الشاق نقص الـ Oxygen على العضلة، مين بيزود بال Oxygen؟ الـ Myoglobin بيكون خزين من ال Oxygen، بيوزح على العضلات و للجسم عند الحاجة. Hormones، بتعمل لبروتينات كهرمون، some hormones are البروتين، بعض الهرمونات هي عبارة عن البروتين، و أشهر مثال على بروتين .. على هرمون يعمل .. يعتبر البروتين هو", "tokens": [28814, 15730, 25724, 38251, 50113, 15040, 8978, 5551, 12984, 1863, 16606, 16712, 29973, 9957, 25124, 995, 4587, 8717, 4587, 9381, 2423, 39184, 16489, 8647, 15844, 18863, 11242, 37977, 12399, 3714, 9957, 4724, 1829, 11622, 23328, 20666, 16489, 8647, 22807, 2423, 39184, 1222, 45841, 13496, 4724, 1829, 30544, 16490, 11622, 9957, 9154, 2423, 16489, 8647, 12399, 4724, 1829, 2407, 11622, 5016, 15844, 18863, 11242, 1211, 9307, 4032, 24976, 7435, 38251, 43242, 21542, 26108, 3660, 13, 389, 687, 2213, 12399, 39894, 25957, 1211, 5296, 26890, 35473, 9957, 9307, 9122, 3224, 2288, 2304, 11536, 12399, 512, 22453, 366, 2423, 26890, 35473, 9957, 12399, 45030, 11242, 2423, 3224, 2288, 2304, 2407, 8315, 2655, 39896, 6225, 3555, 9640, 3660, 18871, 2423, 26890, 35473, 9957, 12399, 4032, 5551, 8592, 3224, 2288, 50113, 6027, 15844, 4724, 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{"start": 359.4, "end": 359.68, "word": " amino", "probability": 0.88427734375}, {"start": 359.68, "end": 360.24, "word": " group", "probability": 0.9833984375}, {"start": 360.24, "end": 360.64, "word": " منه", "probability": 0.6435546875}, {"start": 360.64, "end": 360.94, "word": " حمض", "probability": 0.9051106770833334}, {"start": 360.94, "end": 361.5, "word": " أميني", "probability": 0.893798828125}, {"start": 361.5, "end": 361.88, "word": " مع", "probability": 0.95703125}, {"start": 361.88, "end": 362.0, "word": " ال", "probability": 0.78076171875}, {"start": 362.0, "end": 362.66, "word": " carboxyl", "probability": 0.84326171875}, {"start": 362.66, "end": 363.04, "word": " end", "probability": 0.95654296875}, {"start": 363.04, "end": 363.2, "word": " اللي", "probability": 0.9248046875}, {"start": 363.2, "end": 363.32, "word": " هي", "probability": 0.80810546875}, {"start": 363.32, "end": 363.9, "word": " carboxyl", "probability": 0.8885091145833334}, {"start": 363.9, "end": 364.2, "word": " group", "probability": 0.9697265625}, {"start": 364.2, "end": 364.44, "word": " of", "probability": 0.9677734375}, {"start": 364.44, "end": 364.8, "word": " another", "probability": 0.93212890625}, {"start": 364.8, "end": 365.18, "word": " amino", "probability": 0.98486328125}, {"start": 365.18, "end": 365.5, "word": " acid", "probability": 0.9619140625}], "temperature": 1.0}, {"id": 16, "seek": 39204, "start": 365.9, "end": 392.04, "text": "بتيجي الرابطة باه ال amino group و ال carboxyl group amino group من حمض أميني مع carboxyl group من حمض أميني أخر بتيجي الرابطة باه بتنزع جزء ما و تتكون الرابطة بنسميها it's called a peptide bond this amide bond is called peptide bond وحكينا إنه في حاجة بتنتج منها لما يتكون ال protein بيكون في عندنا حاجة اسمها N-terminus و حاجة اسمها C-terminus", "tokens": [3555, 31371, 7435, 1829, 34892, 16758, 9566, 3660, 4724, 40294, 2423, 24674, 1594, 4032, 2423, 1032, 4995, 5088, 1594, 24674, 1594, 9154, 11331, 2304, 11242, 5551, 2304, 9957, 1829, 20449, 1032, 4995, 5088, 1594, 9154, 11331, 2304, 11242, 5551, 2304, 9957, 1829, 5551, 34740, 4724, 31371, 7435, 1829, 34892, 16758, 9566, 3660, 4724, 40294, 39894, 1863, 11622, 3615, 10874, 11622, 38207, 19446, 4032, 6055, 2655, 30544, 34892, 16758, 9566, 3660, 44945, 38251, 1829, 11296, 309, 311, 1219, 257, 41781, 482, 6086, 341, 669, 482, 6086, 307, 1219, 41781, 482, 6086, 4032, 5016, 4117, 1829, 8315, 36145, 3224, 8978, 11331, 26108, 3660, 39894, 29399, 7435, 9154, 11296, 5296, 15042, 7251, 2655, 30544, 2423, 7944, 4724, 1829, 30544, 8978, 43242, 8315, 11331, 26108, 3660, 24525, 2304, 11296, 426, 12, 29725, 301, 4032, 11331, 26108, 3660, 24525, 2304, 11296, 383, 12, 29725, 301], "avg_logprob": -0.19060283476579273, "compression_ratio": 1.953125, "no_speech_prob": 1.043081283569336e-05, "words": [{"start": 365.9, "end": 366.24, "word": "بتيجي", "probability": 0.898681640625}, {"start": 366.24, "end": 366.86, "word": " الرابطة", "probability": 0.91162109375}, {"start": 366.86, "end": 367.1, "word": " باه", "probability": 0.3348388671875}, {"start": 367.1, "end": 367.52, "word": " ال", "probability": 0.66015625}, {"start": 367.52, "end": 367.86, "word": " amino", "probability": 0.5693359375}, {"start": 367.86, "end": 368.42, "word": " group", "probability": 0.962890625}, {"start": 368.42, "end": 368.74, "word": " و", "probability": 0.84716796875}, {"start": 368.74, "end": 368.96, "word": " ال", "probability": 0.56640625}, {"start": 368.96, "end": 369.64, "word": " carboxyl", "probability": 0.7941080729166666}, {"start": 369.64, "end": 370.0, "word": " group", "probability": 0.9599609375}, {"start": 370.0, "end": 370.36, "word": " amino", "probability": 0.60107421875}, {"start": 370.36, "end": 370.66, "word": " group", "probability": 0.9833984375}, {"start": 370.66, "end": 370.82, "word": " من", "probability": 0.87158203125}, {"start": 370.82, "end": 371.06, "word": " حمض", "probability": 0.9357096354166666}, {"start": 371.06, "end": 371.58, "word": " أميني", "probability": 0.8602294921875}, {"start": 371.58, "end": 371.94, "word": " مع", "probability": 0.87744140625}, {"start": 371.94, "end": 372.5, "word": " carboxyl", "probability": 0.8961588541666666}, {"start": 372.5, "end": 372.76, "word": " group", "probability": 0.86767578125}, {"start": 372.76, "end": 372.92, "word": " من", "probability": 0.97607421875}, {"start": 372.92, "end": 373.16, "word": " حمض", "probability": 0.99267578125}, {"start": 373.16, "end": 373.5, "word": " أميني", "probability": 0.9710693359375}, {"start": 373.5, "end": 373.78, "word": " أخر", "probability": 0.6600341796875}, {"start": 373.78, "end": 374.16, "word": " بتيجي", "probability": 0.8446044921875}, {"start": 374.16, "end": 374.7, "word": " الرابطة", "probability": 0.968505859375}, {"start": 374.7, "end": 374.96, "word": " باه", "probability": 0.92724609375}, {"start": 374.96, "end": 375.8, "word": " بتنزع", "probability": 0.9130859375}, {"start": 375.8, "end": 376.14, "word": " جزء", "probability": 0.7945963541666666}, {"start": 376.14, "end": 376.4, "word": " ما", "probability": 0.666015625}, {"start": 376.4, "end": 376.64, "word": " و", "probability": 0.51416015625}, {"start": 376.64, "end": 377.2, "word": " تتكون", "probability": 0.7853190104166666}, {"start": 377.2, "end": 378.44, "word": " الرابطة", "probability": 0.97998046875}, {"start": 378.44, "end": 379.44, "word": " بنسميها", "probability": 0.85333251953125}, {"start": 379.44, "end": 379.62, "word": " it's", "probability": 0.625732421875}, {"start": 379.62, "end": 380.0, "word": " called", "probability": 0.90673828125}, {"start": 380.0, "end": 380.2, "word": " a", "probability": 0.7783203125}, {"start": 380.2, "end": 380.76, "word": " peptide", "probability": 0.98193359375}, {"start": 380.76, "end": 381.02, "word": " bond", "probability": 0.9482421875}, {"start": 381.02, "end": 381.3, "word": " this", "probability": 0.6865234375}, {"start": 381.3, "end": 381.74, "word": " amide", "probability": 0.9482421875}, {"start": 381.74, "end": 382.04, "word": " bond", "probability": 0.94775390625}, {"start": 382.04, "end": 382.28, "word": " is", "probability": 0.7841796875}, {"start": 382.28, "end": 382.68, "word": " called", "probability": 0.90673828125}, {"start": 382.68, "end": 383.3, "word": " peptide", "probability": 0.8857421875}, {"start": 383.3, "end": 383.78, "word": " bond", "probability": 0.94921875}, {"start": 383.78, "end": 384.58, "word": " وحكينا", "probability": 0.89931640625}, {"start": 384.58, "end": 384.84, "word": " إنه", "probability": 0.4459228515625}, {"start": 384.84, "end": 384.92, "word": " في", "probability": 0.85595703125}, {"start": 384.92, "end": 385.28, "word": " حاجة", "probability": 0.9894205729166666}, {"start": 385.28, "end": 385.68, "word": " بتنتج", "probability": 0.523193359375}, {"start": 385.68, "end": 385.92, "word": " منها", "probability": 0.79248046875}, {"start": 385.92, "end": 386.14, "word": " لما", "probability": 0.9306640625}, {"start": 386.14, "end": 386.54, "word": " يتكون", "probability": 0.8994140625}, {"start": 386.54, "end": 386.7, "word": " ال", "probability": 0.8583984375}, {"start": 386.7, "end": 387.06, "word": " protein", "probability": 0.66845703125}, {"start": 387.06, "end": 387.38, "word": " بيكون", "probability": 0.8426106770833334}, {"start": 387.38, "end": 387.54, "word": " في", "probability": 0.9345703125}, {"start": 387.54, "end": 388.14, "word": " عندنا", "probability": 0.5672607421875}, {"start": 388.14, "end": 388.54, "word": " حاجة", "probability": 0.9959309895833334}, {"start": 388.54, "end": 389.0, "word": " اسمها", "probability": 0.9025065104166666}, {"start": 389.0, "end": 389.34, "word": " N", "probability": 0.90234375}, {"start": 389.34, "end": 389.96, "word": "-terminus", "probability": 0.7464192708333334}, {"start": 389.96, "end": 390.32, "word": " و", "probability": 0.96875}, {"start": 390.32, "end": 390.64, "word": " حاجة", "probability": 0.8702799479166666}, {"start": 390.64, "end": 391.02, "word": " اسمها", "probability": 0.9612630208333334}, {"start": 391.02, "end": 391.34, "word": " C", "probability": 0.90869140625}, {"start": 391.34, "end": 392.04, "word": "-terminus", "probability": 0.9560546875}], "temperature": 1.0}, {"id": 17, "seek": 41299, "start": 392.43, "end": 412.99, "text": "حكينا الـ N-terminus هو الحمض الأميني اللي بيحتوي على amino group لم تشارك في تكوين الرابطة البيبتدية not involved in a peptide bond free amino group اللي بيكون على الطرف فيه free amino group مش مشاركة في تكوين الرابطة البيبتدية بتكون في النهاية بنسميها N-terminus", "tokens": [5016, 4117, 1829, 8315, 2423, 39184, 426, 12, 29725, 301, 31439, 21542, 2304, 11242, 16247, 2304, 9957, 1829, 13672, 1829, 4724, 1829, 33753, 45865, 15844, 24674, 1594, 32767, 6055, 8592, 9640, 4117, 8978, 6055, 4117, 2407, 9957, 34892, 16758, 9566, 3660, 2423, 21292, 3555, 2655, 3215, 10632, 406, 3288, 294, 257, 41781, 482, 6086, 1737, 24674, 1594, 13672, 1829, 4724, 1829, 30544, 15844, 41950, 28480, 8978, 3224, 1737, 24674, 1594, 37893, 37893, 9640, 4117, 3660, 8978, 6055, 4117, 2407, 9957, 34892, 16758, 9566, 3660, 2423, 21292, 3555, 2655, 3215, 10632, 39894, 30544, 8978, 28239, 11296, 10632, 44945, 38251, 1829, 11296, 426, 12, 29725, 301], "avg_logprob": -0.1574404716491699, "compression_ratio": 1.8915094339622642, "no_speech_prob": 1.5020370483398438e-05, "words": [{"start": 392.43, "end": 392.89, "word": "حكينا", "probability": 0.6826171875}, {"start": 392.89, "end": 393.11, "word": " الـ", "probability": 0.84765625}, {"start": 393.11, "end": 393.27, "word": " N", "probability": 0.202880859375}, {"start": 393.27, "end": 393.85, "word": "-terminus", "probability": 0.6016438802083334}, {"start": 393.85, "end": 394.03, "word": " هو", "probability": 0.78564453125}, {"start": 394.03, "end": 394.43, "word": " الحمض", "probability": 0.9254557291666666}, {"start": 394.43, "end": 394.99, "word": " الأميني", "probability": 0.9049072265625}, {"start": 394.99, "end": 395.11, "word": " اللي", "probability": 0.812255859375}, {"start": 395.11, "end": 395.73, "word": " بيحتوي", "probability": 0.9716796875}, {"start": 395.73, "end": 396.21, "word": " على", "probability": 0.890625}, {"start": 396.21, "end": 397.01, "word": " amino", "probability": 0.457763671875}, {"start": 397.01, "end": 397.63, "word": " group", "probability": 0.97607421875}, {"start": 397.63, "end": 398.17, "word": " لم", "probability": 0.9072265625}, {"start": 398.17, "end": 398.85, "word": " تشارك", "probability": 0.9932861328125}, {"start": 398.85, "end": 399.01, "word": " في", "probability": 0.96923828125}, {"start": 399.01, "end": 399.57, "word": " تكوين", "probability": 0.9754638671875}, {"start": 399.57, "end": 400.21, "word": " الرابطة", "probability": 0.886962890625}, {"start": 400.21, "end": 400.89, "word": " البيبتدية", "probability": 0.6906331380208334}, {"start": 400.89, "end": 401.21, "word": " not", "probability": 0.5859375}, {"start": 401.21, "end": 401.97, "word": " involved", "probability": 0.8125}, {"start": 401.97, "end": 402.21, "word": " in", "probability": 0.96630859375}, {"start": 402.21, "end": 402.35, "word": " a", "probability": 0.475830078125}, {"start": 402.35, "end": 402.89, "word": " peptide", "probability": 0.96826171875}, {"start": 402.89, "end": 403.19, "word": " bond", "probability": 0.958984375}, {"start": 403.19, "end": 403.79, "word": " free", "probability": 0.482421875}, {"start": 403.79, "end": 404.23, "word": " amino", "probability": 0.9814453125}, {"start": 404.23, "end": 404.61, "word": " group", "probability": 0.97705078125}, {"start": 404.61, "end": 405.07, "word": " اللي", "probability": 0.97607421875}, {"start": 405.07, "end": 405.33, "word": " بيكون", "probability": 0.9716796875}, {"start": 405.33, "end": 405.53, "word": " على", "probability": 0.88671875}, {"start": 405.53, "end": 405.97, "word": " الطرف", "probability": 0.975341796875}, {"start": 405.97, "end": 406.35, "word": " فيه", "probability": 0.961669921875}, {"start": 406.35, "end": 406.59, "word": " free", "probability": 0.96044921875}, {"start": 406.59, "end": 407.01, "word": " amino", "probability": 0.9814453125}, {"start": 407.01, "end": 407.51, "word": " group", "probability": 0.98583984375}, {"start": 407.51, "end": 407.77, "word": " مش", "probability": 0.974609375}, {"start": 407.77, "end": 408.33, "word": " مشاركة", "probability": 0.9691162109375}, {"start": 408.33, "end": 408.43, "word": " في", "probability": 0.97265625}, {"start": 408.43, "end": 408.85, "word": " تكوين", "probability": 0.9830322265625}, {"start": 408.85, "end": 409.33, "word": " الرابطة", "probability": 0.9423828125}, {"start": 409.33, "end": 410.03, "word": " البيبتدية", "probability": 0.9820149739583334}, {"start": 410.03, "end": 410.39, "word": " بتكون", "probability": 0.8154296875}, {"start": 410.39, "end": 410.51, "word": " في", "probability": 0.92529296875}, {"start": 410.51, "end": 411.11, "word": " النهاية", "probability": 0.97021484375}, {"start": 411.11, "end": 412.11, "word": " بنسميها", "probability": 0.85638427734375}, {"start": 412.11, "end": 412.45, "word": " N", "probability": 0.9208984375}, {"start": 412.45, "end": 412.99, "word": "-terminus", "probability": 0.87353515625}], "temperature": 1.0}, {"id": 18, "seek": 44059, "start": 413.45, "end": 440.59, "text": "الـ C-terminus هي الحمض الأمين اللي بيحتوي على كربوكسيل جروب not involved in a peptide bond يحتوي على كربوكسيل جروب لم تشارك في تكوين الرابطة البيبتدية free carboxyl group طب إيش لنا ال protein structure؟ و أكيد أخدته بالنظرى في منه primary structure secondary structure tertiary و quaternary", "tokens": [6027, 39184, 383, 12, 29725, 301, 39896, 21542, 2304, 11242, 16247, 2304, 9957, 13672, 1829, 4724, 1829, 33753, 45865, 15844, 9122, 25513, 2407, 4117, 3794, 26895, 10874, 32887, 3555, 406, 3288, 294, 257, 41781, 482, 6086, 7251, 33753, 45865, 15844, 9122, 25513, 2407, 4117, 3794, 26895, 10874, 32887, 3555, 32767, 6055, 8592, 9640, 4117, 8978, 6055, 4117, 2407, 9957, 34892, 16758, 9566, 3660, 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group الشحنات اللي عليهم هكذا بتختلف حسب الوسط اللي أنا بحطها فيه هل هي في وسط حمضي، في وسط قاعدي، في وسط متعدد فأنا خصائص الحمض الأميني بدي أحددها من خلال الـ R group وشحنة الـ Amino group and Carboxyl group", "tokens": [6027, 39184, 2012, 2982, 1594, 16070, 39184, 2741, 4995, 5088, 1594, 25124, 5016, 8315, 2655, 13672, 1829, 25894, 16095, 8032, 4117, 15730, 39894, 46456, 46538, 11331, 35457, 2423, 41779, 9566, 13672, 1829, 41850, 4724, 5016, 9566, 11296, 8978, 3224, 8032, 1211, 39896, 8978, 46952, 9566, 11331, 2304, 11242, 1829, 12399, 8978, 46952, 9566, 12174, 995, 22488, 1829, 12399, 8978, 46952, 9566, 44650, 22488, 3215, 6156, 10721, 8315, 16490, 9381, 16373, 9381, 21542, 2304, 11242, 16247, 2304, 9957, 1829, 4724, 16254, 5551, 24401, 3215, 11296, 9154, 16490, 1211, 6027, 2423, 39184, 497, 1594, 4032, 8592, 5016, 1863, 3660, 2423, 39184, 2012, 2982, 1594, 293, 2741, 4995, 5088, 1594], "avg_logprob": -0.12745948494584472, "compression_ratio": 1.8076923076923077, "no_speech_prob": 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بيئات في acidic ولا في alkaline ولا في neutral طب مين صار عليها protonated؟", "tokens": [3615, 8592, 7649, 39896, 4117, 4724, 1829, 5016, 4117, 1829, 20292, 309, 311, 1021, 281, 458, 597, 1594, 295, 41781, 1875, 293, 15577, 486, 312, 31728, 770, 412, 1629, 4724, 1829, 19986, 9307, 6156, 11242, 2288, 13063, 1829, 16472, 1975, 3615, 28480, 1975, 8315, 3714, 29245, 23328, 3660, 8978, 1975, 1829, 46952, 9566, 8978, 1975, 1829, 4724, 1829, 19986, 9307, 8978, 39514, 49429, 8978, 44220, 533, 49429, 8978, 10598, 23032, 3555, 3714, 9957, 20328, 9640, 25894, 11296, 31728, 770, 22807], "avg_logprob": -0.1402391916439857, "compression_ratio": 1.471698113207547, "no_speech_prob": 1.6689300537109375e-06, "words": [{"start": 600.02, "end": 600.58, "word": "عشان", "probability": 0.9627278645833334}, {"start": 600.58, "end": 600.94, "word": " هيك", "probability": 0.9345703125}, {"start": 600.94, "end": 601.68, "word": " بيحكيلي", "probability": 0.9226888020833334}, {"start": 601.68, "end": 602.06, 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"probability": 0.942138671875}, {"start": 607.72, "end": 608.1, "word": " at", "probability": 0.8369140625}, {"start": 608.1, "end": 608.64, "word": " certain", "probability": 0.91357421875}, {"start": 608.64, "end": 609.1, "word": " بيئات", "probability": 0.8916015625}, {"start": 609.1, "end": 610.46, "word": " فضروري", "probability": 0.9298828125}, {"start": 610.46, "end": 610.78, "word": " ان", "probability": 0.292724609375}, {"start": 610.78, "end": 611.46, "word": " اعرف", "probability": 0.9459635416666666}, {"start": 611.46, "end": 611.92, "word": " انا", "probability": 0.796142578125}, {"start": 611.92, "end": 612.42, "word": " موجودة", "probability": 0.984619140625}, {"start": 612.42, "end": 612.58, "word": " في", "probability": 0.96533203125}, {"start": 612.58, "end": 612.86, "word": " اي", "probability": 0.855224609375}, {"start": 612.86, "end": 613.36, "word": " وسط", "probability": 0.844970703125}, {"start": 613.36, "end": 613.52, "word": " في", "probability": 0.5849609375}, {"start": 613.52, "end": 613.76, "word": " اي", "probability": 0.93212890625}, {"start": 613.76, "end": 614.34, "word": " بيئات", "probability": 0.9776611328125}, {"start": 614.34, "end": 614.74, "word": " في", "probability": 0.81689453125}, {"start": 614.74, "end": 615.14, "word": " acidic", "probability": 0.8720703125}, {"start": 615.14, "end": 615.42, "word": " ولا", "probability": 0.61962890625}, {"start": 615.42, "end": 615.6, "word": " في", "probability": 0.974609375}, {"start": 615.6, "end": 616.16, "word": " alkaline", "probability": 0.978515625}, {"start": 616.16, "end": 616.3, "word": " ولا", "probability": 0.966796875}, {"start": 616.3, "end": 616.44, "word": " في", "probability": 0.9599609375}, {"start": 616.44, "end": 616.9, "word": " neutral", "probability": 0.9755859375}, {"start": 616.9, "end": 617.52, "word": " طب", "probability": 0.775146484375}, {"start": 617.52, "end": 617.74, "word": " مين", "probability": 0.943359375}, {"start": 617.74, "end": 617.92, "word": " صار", "probability": 0.963134765625}, {"start": 617.92, "end": 618.2, "word": " عليها", "probability": 0.98095703125}, {"start": 618.2, "end": 619.2, "word": " protonated؟", "probability": 0.92822265625}], "temperature": 1.0}, {"id": 26, "seek": 63426, "start": 619.24, "end": 634.26, "text": "ومين صار لها deprotonated؟ مين فقدت ومين كسبت؟ الشحنة هنا صارت جديش النت ت charge وهنا جديش النت ت charge لأن حاجة الشحنة بتحدد خصائص الحمض الأميني وبالتالي هيعرف خاصية خصائص البروتين", "tokens": [20498, 9957, 20328, 9640, 5296, 11296, 1367, 10536, 266, 770, 22807, 3714, 9957, 6156, 28543, 2655, 4032, 2304, 9957, 9122, 35457, 2655, 22807, 25124, 5016, 1863, 3660, 34105, 20328, 9640, 2655, 10874, 16254, 8592, 2423, 29399, 6055, 4602, 37037, 8315, 10874, 16254, 8592, 2423, 29399, 6055, 4602, 5296, 33456, 11331, 26108, 3660, 25124, 5016, 1863, 3660, 39894, 24401, 3215, 16490, 9381, 16373, 9381, 21542, 2304, 11242, 16247, 2304, 9957, 1829, 46599, 6027, 2655, 6027, 1829, 39896, 3615, 28480, 16490, 33546, 10632, 16490, 9381, 16373, 9381, 2423, 26890, 35473, 9957], "avg_logprob": -0.19461805704567167, "compression_ratio": 1.7277777777777779, "no_speech_prob": 2.4437904357910156e-06, "words": [{"start": 619.24, "end": 619.66, "word": "ومين", "probability": 0.819091796875}, {"start": 619.66, "end": 619.92, "word": " صار", "probability": 0.924072265625}, {"start": 619.92, "end": 620.18, "word": " لها", "probability": 0.6826171875}, {"start": 620.18, "end": 621.5, "word": " deprotonated؟", "probability": 0.742822265625}, {"start": 621.5, "end": 621.9, "word": " مين", "probability": 0.95166015625}, {"start": 621.9, "end": 622.48, "word": " فقدت", "probability": 0.9934895833333334}, {"start": 622.48, "end": 622.78, "word": " ومين", "probability": 0.86083984375}, {"start": 622.78, "end": 623.6, "word": " كسبت؟", "probability": 0.9761962890625}, {"start": 623.6, "end": 624.14, "word": " الشحنة", "probability": 0.82537841796875}, {"start": 624.14, "end": 624.3, "word": " هنا", "probability": 0.51171875}, {"start": 624.3, "end": 624.72, "word": " صارت", "probability": 0.9422200520833334}, {"start": 624.72, "end": 625.1, "word": " جديش", "probability": 0.7034505208333334}, {"start": 625.1, "end": 625.34, "word": " النت", "probability": 0.579833984375}, {"start": 625.34, "end": 625.52, "word": " ت", "probability": 0.87939453125}, {"start": 625.52, "end": 625.86, "word": " charge", "probability": 0.40380859375}, {"start": 625.86, "end": 626.16, "word": " وهنا", "probability": 0.714599609375}, {"start": 626.16, "end": 626.58, "word": " جديش", "probability": 0.9772135416666666}, {"start": 626.58, "end": 626.86, "word": " النت", "probability": 0.844482421875}, {"start": 626.86, "end": 627.02, "word": " ت", "probability": 0.9345703125}, {"start": 627.02, "end": 627.34, "word": " charge", "probability": 0.65087890625}, {"start": 627.34, "end": 627.94, "word": " لأن", "probability": 0.730712890625}, {"start": 627.94, "end": 628.3, "word": " حاجة", "probability": 0.603759765625}, {"start": 628.3, "end": 628.9, "word": " الشحنة", "probability": 0.9796142578125}, {"start": 628.9, "end": 629.48, "word": " بتحدد", "probability": 0.9334309895833334}, {"start": 629.48, "end": 630.2, "word": " خصائص", "probability": 0.9852294921875}, {"start": 630.2, "end": 630.64, "word": " الحمض", "probability": 0.9554036458333334}, {"start": 630.64, "end": 631.36, "word": " الأميني", "probability": 0.8621826171875}, {"start": 631.36, "end": 632.1, "word": " وبالتالي", "probability": 0.9404296875}, {"start": 632.1, "end": 632.42, "word": " هيعرف", "probability": 0.8614908854166666}, {"start": 632.42, "end": 632.96, "word": " خاصية", "probability": 0.783203125}, {"start": 632.96, "end": 633.64, "word": " خصائص", "probability": 0.9105224609375}, {"start": 633.64, "end": 634.26, "word": " البروتين", "probability": 0.9464111328125}], "temperature": 1.0}, {"id": 27, "seek": 65149, "start": 634.95, "end": 651.49, "text": "protein molecules carry charge according to their amino acid sequence and the aqueous solvent pH they are dissolved in بحكيلي شحنة البروتين كيف بده أحددها من خلال الأحماض الأمينية المكونة لها ال amino acid sequence", "tokens": [1424, 1370, 259, 13093, 3985, 4602, 4650, 281, 641, 24674, 8258, 8310, 293, 264, 257, 1077, 563, 33575, 21677, 436, 366, 30651, 294, 4724, 5016, 4117, 1829, 20292, 13412, 5016, 1863, 3660, 2423, 26890, 35473, 9957, 9122, 33911, 47525, 3224, 5551, 24401, 3215, 11296, 9154, 16490, 1211, 6027, 16247, 35571, 46958, 16247, 2304, 9957, 10632, 9673, 30544, 3660, 5296, 11296, 2423, 24674, 8258, 8310], "avg_logprob": -0.1795673076923077, "compression_ratio": 1.4656084656084656, "no_speech_prob": 1.1920928955078125e-06, "words": [{"start": 634.95, "end": 635.61, "word": "protein", "probability": 0.69708251953125}, {"start": 635.61, "end": 636.41, "word": " molecules", "probability": 0.7275390625}, {"start": 636.41, "end": 636.75, "word": " carry", "probability": 0.93701171875}, {"start": 636.75, "end": 637.29, "word": " charge", "probability": 0.94140625}, {"start": 637.29, "end": 637.83, "word": " according", "probability": 0.93994140625}, {"start": 637.83, "end": 638.15, "word": " to", "probability": 0.9453125}, {"start": 638.15, "end": 638.39, "word": " their", "probability": 0.94677734375}, {"start": 638.39, "end": 638.75, "word": " amino", "probability": 0.9658203125}, {"start": 638.75, "end": 639.27, "word": " acid", "probability": 0.880859375}, {"start": 639.27, "end": 640.09, "word": " sequence", "probability": 0.8955078125}, {"start": 640.09, "end": 640.67, "word": " and", "probability": 0.880859375}, {"start": 640.67, "end": 640.85, "word": " the", "probability": 0.64501953125}, {"start": 640.85, "end": 641.33, "word": " aqueous", "probability": 0.7815755208333334}, {"start": 641.33, "end": 641.83, "word": " solvent", "probability": 0.83056640625}, {"start": 641.83, "end": 642.37, "word": " pH", "probability": 0.74951171875}, {"start": 642.37, "end": 642.71, "word": " they", "probability": 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{"start": 717.41, "end": 717.61, "word": " in", "probability": 0.93701171875}, {"start": 717.61, "end": 717.93, "word": " water", "probability": 0.93359375}, {"start": 717.93, "end": 718.17, "word": " أو", "probability": 0.900390625}, {"start": 718.17, "end": 718.41, "word": " not", "probability": 0.826171875}, {"start": 718.41, "end": 718.95, "word": " soluble", "probability": 0.908447265625}, {"start": 718.95, "end": 719.71, "word": " the", "probability": 0.19921875}, {"start": 719.71, "end": 720.03, "word": " most", "probability": 0.90234375}, {"start": 720.03, "end": 720.57, "word": " important", "probability": 0.94775390625}, {"start": 720.57, "end": 721.45, "word": " determinants", "probability": 0.6796875}, {"start": 721.45, "end": 721.83, "word": " is", "probability": 0.38330078125}, {"start": 721.83, "end": 723.07, "word": " electrostatic", "probability": 0.9000651041666666}, {"start": 723.07, "end": 723.55, "word": " charge", "probability": 0.96240234375}, {"start": 723.55, "end": 724.05, "word": " أهم", "probability": 0.973388671875}, {"start": 724.05, "end": 724.49, "word": " عامل", "probability": 0.9952799479166666}, {"start": 724.49, "end": 724.99, "word": " بحد", "probability": 0.924072265625}, {"start": 724.99, "end": 725.63, "word": " ذائبية", "probability": 0.949951171875}, {"start": 725.63, "end": 726.29, "word": " البروتين", "probability": 0.98193359375}, {"start": 726.29, "end": 726.89, "word": " هي", "probability": 0.9482421875}, {"start": 726.89, "end": 727.47, "word": " شحنة", "probability": 0.9703369140625}, {"start": 727.47, "end": 728.07, "word": " البروتين", "probability": 0.9871826171875}], "temperature": 1.0}, {"id": 31, "seek": 75076, "start": 729.18, "end": 750.76, "text": "إذا كان البروتين مثلا شحنته موجبة أو شحنته سلب، انت تشارجي اللي عليه جداش Solubility of البروتين كمان بتعتمد على distribution of hydrophilic and hydrophobic amino acid on the protein surface، هي اللي بحدد شحنة البروتين حكينا هي ال amino acid sequence، ترتيب الأحمار الأمينية اللي عليها", "tokens": [28814, 15730, 25961, 2423, 26890, 35473, 9957, 50113, 15040, 13412, 5016, 1863, 47395, 3714, 29245, 49401, 34051, 13412, 5016, 29399, 3224, 8608, 46152, 12399, 16472, 2655, 6055, 8592, 9640, 7435, 1829, 13672, 1829, 47356, 10874, 28259, 8592, 7026, 836, 1140, 295, 2423, 26890, 35473, 9957, 9122, 2304, 7649, 39894, 34268, 2304, 3215, 15844, 7316, 295, 5796, 11741, 388, 299, 293, 5796, 11741, 22234, 24674, 8258, 322, 264, 7944, 3753, 12399, 39896, 13672, 1829, 4724, 24401, 3215, 13412, 5016, 1863, 3660, 2423, 26890, 35473, 9957, 11331, 4117, 1829, 8315, 39896, 2423, 24674, 8258, 8310, 12399, 6055, 2288, 31371, 3555, 16247, 35571, 9640, 16247, 2304, 9957, 10632, 13672, 1829, 25894, 11296], "avg_logprob": -0.25653409497304397, "compression_ratio": 1.7469387755102042, "no_speech_prob": 3.4570693969726562e-06, "words": [{"start": 729.18, "end": 729.46, "word": "إذا", "probability": 0.714599609375}, {"start": 729.46, "end": 729.7, "word": " كان", "probability": 0.99365234375}, {"start": 729.7, "end": 730.2, "word": " البروتين", "probability": 0.8341064453125}, {"start": 730.2, "end": 730.56, "word": " مثلا", "probability": 0.595947265625}, {"start": 730.56, "end": 731.1, "word": " شحنته", "probability": 0.9034423828125}, {"start": 731.1, "end": 731.52, "word": " موجبة", "probability": 0.8190104166666666}, {"start": 731.52, "end": 731.72, "word": " أو", "probability": 0.9453125}, {"start": 731.72, "end": 732.24, "word": " شحنته", "probability": 0.868896484375}, {"start": 732.24, "end": 732.54, "word": " سلب،", "probability": 0.5740559895833334}, {"start": 732.54, "end": 732.8, "word": " انت", "probability": 0.45404052734375}, {"start": 732.8, "end": 733.26, "word": " تشارجي", "probability": 0.751220703125}, {"start": 733.26, "end": 733.36, "word": " اللي", "probability": 0.901123046875}, {"start": 733.36, "end": 733.62, "word": " عليه", "probability": 0.58837890625}, {"start": 733.62, "end": 734.32, "word": " جداش", "probability": 0.65625}, {"start": 734.32, "end": 735.54, "word": " Solubility", "probability": 0.7235107421875}, {"start": 735.54, "end": 735.76, "word": " of", "probability": 0.94970703125}, {"start": 735.76, "end": 736.28, "word": " البروتين", "probability": 0.93701171875}, {"start": 736.28, "end": 736.66, "word": " كمان", "probability": 0.9368489583333334}, {"start": 736.66, "end": 737.36, "word": " بتعتمد", "probability": 0.91015625}, {"start": 737.36, "end": 737.74, "word": " على", "probability": 0.89599609375}, {"start": 737.74, "end": 739.0, "word": " distribution", "probability": 0.7724609375}, {"start": 739.0, "end": 739.38, "word": " of", "probability": 0.9580078125}, {"start": 739.38, "end": 740.3, "word": " hydrophilic", "probability": 0.8543701171875}, {"start": 740.3, "end": 740.78, "word": " and", "probability": 0.76806640625}, {"start": 740.78, "end": 741.66, "word": " hydrophobic", "probability": 0.8953450520833334}, {"start": 741.66, "end": 742.18, "word": " amino", "probability": 0.978515625}, {"start": 742.18, "end": 742.7, "word": " acid", "probability": 0.86083984375}, {"start": 742.7, "end": 743.02, "word": " on", "probability": 0.6064453125}, {"start": 743.02, "end": 743.2, "word": " the", "probability": 0.83154296875}, {"start": 743.2, "end": 743.56, "word": " protein", "probability": 0.931640625}, {"start": 743.56, "end": 744.76, "word": " surface،", "probability": 0.52740478515625}, {"start": 744.76, "end": 744.82, "word": " هي", "probability": 0.303955078125}, {"start": 744.82, "end": 745.0, "word": " اللي", "probability": 0.93798828125}, {"start": 745.0, "end": 745.46, "word": " بحدد", "probability": 0.89111328125}, {"start": 745.46, "end": 745.84, "word": " شحنة", "probability": 0.9635009765625}, {"start": 745.84, "end": 746.34, "word": " البروتين", "probability": 0.9833984375}, {"start": 746.34, "end": 746.92, "word": " حكينا", "probability": 0.68829345703125}, {"start": 746.92, "end": 747.46, "word": " هي", "probability": 0.5537109375}, {"start": 747.46, "end": 747.6, "word": " ال", "probability": 0.9794921875}, {"start": 747.6, "end": 747.94, "word": " amino", "probability": 0.39892578125}, {"start": 747.94, "end": 748.24, "word": " acid", "probability": 0.93505859375}, {"start": 748.24, "end": 748.92, "word": " sequence،", "probability": 0.82568359375}, {"start": 748.92, "end": 749.28, "word": " ترتيب", "probability": 0.8509521484375}, {"start": 749.28, "end": 749.68, "word": " الأحمار", "probability": 0.7884114583333334}, {"start": 749.68, "end": 750.28, "word": " الأمينية", "probability": 0.8536376953125}, {"start": 750.28, "end": 750.44, "word": " اللي", "probability": 0.898193359375}, {"start": 750.44, "end": 750.76, "word": " عليها", "probability": 0.73291015625}], "temperature": 1.0}, {"id": 32, "seek": 78146, "start": 752.44, "end": 781.46, "text": "هذه الشحنة كمان هتحدد الـ solubility للبروتين من خلال توزيع الـhydrophilic وhydrophobic أمينو أسد الموجودين على البروتين البروتين هو عبارة عن sequence من أمينو أسد هتطلع هاي الأحمض الأمينية شو شحنتها هتطلع إيش الأحمض الأمينية المكونة له هل هي hydrophilic ولا hydrophobic مين الأغلب عنده hydrophilic ولا hydrophobic موجود الـhydrophobic مثلا للداخل متخبيه والـhydrophilic للخارج", "tokens": [3224, 24192, 25124, 5016, 1863, 3660, 9122, 2304, 7649, 8032, 2655, 24401, 3215, 2423, 39184, 1404, 836, 1140, 24976, 26890, 35473, 9957, 9154, 16490, 1211, 6027, 33427, 11622, 40228, 2423, 39184, 21591, 11741, 388, 299, 4032, 21591, 11741, 22234, 5551, 2304, 9957, 2407, 5551, 3794, 3215, 9673, 29245, 23328, 9957, 15844, 2423, 26890, 35473, 9957, 2423, 26890, 35473, 9957, 31439, 6225, 3555, 9640, 3660, 18871, 8310, 9154, 5551, 2304, 9957, 2407, 5551, 3794, 3215, 8032, 2655, 9566, 1211, 3615, 8032, 47302, 16247, 35571, 11242, 16247, 2304, 9957, 10632, 13412, 2407, 13412, 5016, 29399, 11296, 8032, 2655, 9566, 1211, 3615, 11933, 1829, 8592, 16247, 35571, 11242, 16247, 2304, 9957, 10632, 9673, 30544, 3660, 46740, 8032, 1211, 39896, 5796, 11741, 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0.8465576171875}, {"start": 755.68, "end": 756.32, "word": " من", "probability": 0.93994140625}, {"start": 756.32, "end": 756.9, "word": " خلال", "probability": 0.9972330729166666}, {"start": 756.9, "end": 757.66, "word": " توزيع", "probability": 0.8216145833333334}, {"start": 757.66, "end": 758.88, "word": " الـhydrophilic", "probability": 0.7279052734375}, {"start": 758.88, "end": 759.78, "word": " وhydrophobic", "probability": 0.83319091796875}, {"start": 759.78, "end": 760.3, "word": " أمينو", "probability": 0.79266357421875}, {"start": 760.3, "end": 760.6, "word": " أسد", "probability": 0.8444010416666666}, {"start": 760.6, "end": 761.28, "word": " الموجودين", "probability": 0.9798583984375}, {"start": 761.28, "end": 761.44, "word": " على", "probability": 0.859375}, {"start": 761.44, "end": 762.0, "word": " البروتين", "probability": 0.9617919921875}, {"start": 762.0, "end": 763.14, "word": " البروتين", "probability": 0.9664306640625}, {"start": 763.14, "end": 763.3, "word": " هو", "probability": 0.87451171875}, {"start": 763.3, "end": 763.64, "word": " عبارة", "probability": 0.99609375}, {"start": 763.64, "end": 763.84, "word": " عن", "probability": 0.9921875}, {"start": 763.84, "end": 764.36, "word": " sequence", "probability": 0.62451171875}, {"start": 764.36, "end": 764.6, "word": " من", "probability": 0.99267578125}, {"start": 764.6, "end": 764.98, "word": " أمينو", "probability": 0.9052734375}, {"start": 764.98, "end": 765.34, "word": " أسد", "probability": 0.9905598958333334}, {"start": 765.34, "end": 766.16, "word": " هتطلع", "probability": 0.84375}, {"start": 766.16, "end": 766.56, "word": " هاي", "probability": 0.6640625}, {"start": 766.56, "end": 767.04, "word": " الأحمض", "probability": 0.8326822916666666}, {"start": 767.04, "end": 767.5, "word": " الأمينية", "probability": 0.900146484375}, {"start": 767.5, "end": 767.66, "word": " شو", "probability": 0.955322265625}, {"start": 767.66, "end": 768.26, "word": " شحنتها", "probability": 0.9727783203125}, {"start": 768.26, "end": 769.28, "word": " هتطلع", "probability": 0.88974609375}, {"start": 769.28, "end": 769.6, "word": " إيش", "probability": 0.7861328125}, {"start": 769.6, "end": 770.04, "word": " الأحمض", "probability": 0.9840494791666666}, {"start": 770.04, "end": 770.4, "word": " الأمينية", "probability": 0.966796875}, {"start": 770.4, "end": 770.92, "word": " المكونة", "probability": 0.8582356770833334}, {"start": 770.92, "end": 771.1, "word": " له", "probability": 0.52294921875}, {"start": 771.1, "end": 771.38, "word": " هل", "probability": 0.844482421875}, {"start": 771.38, "end": 771.54, "word": " هي", "probability": 0.76318359375}, {"start": 771.54, "end": 772.42, "word": " hydrophilic", "probability": 0.85296630859375}, {"start": 772.42, "end": 772.92, "word": " ولا", "probability": 0.71240234375}, {"start": 772.92, "end": 773.74, "word": " hydrophobic", "probability": 0.8595377604166666}, {"start": 773.74, "end": 774.34, "word": " مين", "probability": 0.754150390625}, {"start": 774.34, "end": 774.78, "word": " الأغلب", "probability": 0.9894205729166666}, {"start": 774.78, "end": 775.08, "word": " عنده", "probability": 0.984375}, {"start": 775.08, "end": 775.9, "word": " hydrophilic", "probability": 0.7962646484375}, {"start": 775.9, "end": 776.28, "word": " ولا", "probability": 0.8310546875}, {"start": 776.28, "end": 777.0, "word": " hydrophobic", "probability": 0.94921875}, {"start": 777.0, "end": 777.68, "word": " موجود", "probability": 0.9267578125}, {"start": 777.68, "end": 778.38, "word": " الـhydrophobic", "probability": 0.77958984375}, {"start": 778.38, "end": 778.78, "word": " مثلا", "probability": 0.964111328125}, {"start": 778.78, "end": 779.42, "word": " للداخل", "probability": 0.97314453125}, {"start": 779.42, "end": 780.0, "word": " متخبيه", "probability": 0.7855224609375}, {"start": 780.0, "end": 780.76, "word": " والـhydrophilic", "probability": 0.7766462053571429}, {"start": 780.76, "end": 781.46, "word": " للخارج", "probability": 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"word": " the", "probability": 0.91064453125}, {"start": 791.22, "end": 791.86, "word": " surface", "probability": 0.86865234375}, {"start": 791.86, "end": 792.68, "word": " have", "probability": 0.7626953125}, {"start": 792.68, "end": 793.46, "word": " low", "probability": 0.74462890625}, {"start": 793.46, "end": 794.36, "word": " solubility", "probability": 0.96826171875}, {"start": 794.36, "end": 794.56, "word": " in", "probability": 0.86181640625}, {"start": 794.56, "end": 795.04, "word": " aqueous", "probability": 0.41790771484375}, {"start": 795.04, "end": 795.44, "word": " solvent", "probability": 0.779296875}, {"start": 795.44, "end": 796.3, "word": " فجدت", "probability": 0.481353759765625}, {"start": 796.3, "end": 797.1, "word": " hydrophobic", "probability": 0.7979329427083334}, {"start": 797.1, "end": 797.7, "word": " معناها", "probability": 0.93603515625}, {"start": 797.7, "end": 798.4, "word": " كاره", "probability": 0.9754231770833334}, {"start": 798.4, "end": 798.92, "word": " للماء", "probability": 0.9365234375}], "temperature": 1.0}, {"id": 34, "seek": 82675, "start": 799.43, "end": 826.75, "text": "Hydrophobic كاره للماء فإذا البروتين بيتكون من أحماض أمينية كارهة للماء وده كرمية كبيرة من أحماض أمينية كارهة للماء موجودة على السطح فهذا بيقلل الذائبية على مبدأ like dissolve like المديبات مديب بعضها اللي لهم نفس الخصائص بدوبوا ببعض إذا كان هذا الحمض الأميني كاره للماء كيف بيدوب في الماء إذا هيكون له solubility", "tokens": [39, 6655, 11741, 22234, 9122, 9640, 3224, 5296, 19528, 16606, 6156, 28814, 15730, 2423, 26890, 35473, 9957, 4724, 36081, 30544, 9154, 5551, 35571, 46958, 5551, 2304, 9957, 10632, 9122, 9640, 3224, 3660, 5296, 19528, 16606, 4032, 3215, 3224, 9122, 2288, 2304, 10632, 9122, 3555, 48923, 9154, 5551, 35571, 46958, 5551, 2304, 9957, 10632, 9122, 9640, 3224, 3660, 5296, 19528, 16606, 3714, 29245, 23328, 3660, 15844, 21136, 9566, 5016, 6156, 3224, 15730, 4724, 1829, 4587, 1211, 1211, 32545, 16373, 3555, 10632, 15844, 3714, 44510, 10721, 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"word": " كارهة", "probability": 0.9881591796875}, {"start": 809.69, "end": 810.09, "word": " للماء", "probability": 0.9527994791666666}, {"start": 810.09, "end": 810.71, "word": " موجودة", "probability": 0.9656982421875}, {"start": 810.71, "end": 810.93, "word": " على", "probability": 0.92529296875}, {"start": 810.93, "end": 811.63, "word": " السطح", "probability": 0.9462890625}, {"start": 811.63, "end": 812.65, "word": " فهذا", "probability": 0.9716796875}, {"start": 812.65, "end": 813.55, "word": " بيقلل", "probability": 0.92353515625}, {"start": 813.55, "end": 814.45, "word": " الذائبية", "probability": 0.9359130859375}, {"start": 814.45, "end": 814.87, "word": " على", "probability": 0.88427734375}, {"start": 814.87, "end": 815.45, "word": " مبدأ", "probability": 0.92431640625}, {"start": 815.45, "end": 815.93, "word": " like", "probability": 0.1478271484375}, {"start": 815.93, "end": 816.43, "word": " dissolve", "probability": 0.1815185546875}, {"start": 816.43, "end": 817.03, "word": " like", "probability": 0.95458984375}, {"start": 817.03, "end": 817.97, "word": " المديبات", "probability": 0.7823486328125}, {"start": 817.97, "end": 818.39, "word": " مديب", "probability": 0.5205078125}, {"start": 818.39, "end": 818.75, "word": " بعضها", "probability": 0.8450520833333334}, {"start": 818.75, "end": 818.91, "word": " اللي", "probability": 0.935546875}, {"start": 818.91, "end": 819.09, "word": " لهم", "probability": 0.6634521484375}, {"start": 819.09, "end": 819.39, "word": " نفس", "probability": 0.98779296875}, {"start": 819.39, "end": 819.89, "word": " الخصائص", "probability": 0.9478759765625}, {"start": 819.89, "end": 820.29, "word": " بدوبوا", "probability": 0.76513671875}, {"start": 820.29, "end": 820.65, "word": " ببعض", "probability": 0.84320068359375}, {"start": 820.65, "end": 821.43, "word": " إذا", "probability": 0.82763671875}, {"start": 821.43, "end": 821.73, "word": " كان", "probability": 0.9873046875}, {"start": 821.73, "end": 821.99, "word": " هذا", "probability": 0.96630859375}, {"start": 821.99, "end": 822.31, "word": " الحمض", "probability": 0.9329427083333334}, {"start": 822.31, "end": 822.69, "word": " الأميني", "probability": 0.904052734375}, {"start": 822.69, "end": 822.97, "word": " كاره", "probability": 0.9957682291666666}, {"start": 822.97, "end": 823.35, "word": " للماء", "probability": 0.96533203125}, {"start": 823.35, "end": 823.65, "word": " كيف", "probability": 0.853759765625}, {"start": 823.65, "end": 823.99, "word": " بيدوب", "probability": 0.7662760416666666}, {"start": 823.99, "end": 824.13, "word": " في", "probability": 0.93359375}, {"start": 824.13, "end": 824.49, "word": " الماء", "probability": 0.993408203125}, {"start": 824.49, "end": 825.05, "word": " إذا", "probability": 0.718017578125}, {"start": 825.05, "end": 825.63, "word": " هيكون", "probability": 0.771728515625}, {"start": 825.63, "end": 825.91, "word": " له", "probability": 0.69287109375}, {"start": 825.91, "end": 826.75, "word": " solubility", "probability": 0.8606770833333334}], "temperature": 1.0}, {"id": 35, "seek": 84176, "start": 831.36, "end": 841.76, "text": "بحكي ليه هناك هي أن أمثلة على Hydrophilic Amino Acid و Hydrophobic Amino Acid زي مين Hydrophilic و مين Hydrophobic و هذا مش مطلوب أنها نفسها", "tokens": [49628, 4117, 1829, 32239, 3224, 34105, 4117, 39896, 14739, 5551, 2304, 12984, 37977, 15844, 24231, 11741, 388, 299, 2012, 2982, 5097, 327, 4032, 24231, 11741, 22234, 2012, 2982, 5097, 327, 30767, 1829, 3714, 9957, 24231, 11741, 388, 299, 4032, 3714, 9957, 24231, 11741, 22234, 4032, 23758, 37893, 3714, 9566, 1211, 37746, 14739, 11296, 8717, 36178, 11296], "avg_logprob": -0.27713816207751896, "compression_ratio": 1.4296296296296296, "no_speech_prob": 1.1920928955078125e-07, "words": [{"start": 831.36, "end": 831.96, "word": "بحكي", "probability": 0.8185221354166666}, {"start": 831.96, "end": 832.44, "word": " ليه", "probability": 0.9326171875}, {"start": 832.44, "end": 832.98, "word": " هناك", "probability": 0.4962158203125}, {"start": 832.98, "end": 833.12, "word": " هي", "probability": 0.34423828125}, {"start": 833.12, "end": 833.22, "word": " أن", "probability": 0.60205078125}, {"start": 833.22, "end": 833.7, "word": " أمثلة", "probability": 0.9024658203125}, {"start": 833.7, "end": 833.9, "word": " على", "probability": 0.853515625}, {"start": 833.9, "end": 834.6, "word": " Hydrophilic", "probability": 0.670684814453125}, {"start": 834.6, "end": 834.94, "word": " Amino", "probability": 0.67822265625}, {"start": 834.94, "end": 835.38, "word": " Acid", "probability": 0.775390625}, {"start": 835.38, "end": 835.48, "word": " و", "probability": 0.91162109375}, {"start": 835.48, "end": 836.14, "word": " Hydrophobic", "probability": 0.83349609375}, {"start": 836.14, "end": 836.5, "word": " Amino", "probability": 0.968994140625}, {"start": 836.5, "end": 836.82, "word": " Acid", "probability": 0.977294921875}, {"start": 836.82, "end": 837.08, "word": " زي", "probability": 0.8232421875}, {"start": 837.08, "end": 837.4, "word": " مين", "probability": 0.933349609375}, {"start": 837.4, "end": 838.5, "word": " Hydrophilic", "probability": 0.8780517578125}, {"start": 838.5, "end": 838.6, "word": " و", "probability": 0.97265625}, {"start": 838.6, "end": 838.78, "word": " مين", "probability": 0.88232421875}, {"start": 838.78, "end": 839.7, "word": " Hydrophobic", "probability": 0.8831380208333334}, {"start": 839.7, "end": 840.1, "word": " و", "probability": 0.496337890625}, {"start": 840.1, "end": 840.22, "word": " هذا", "probability": 0.64453125}, {"start": 840.22, "end": 840.38, "word": " مش", "probability": 0.96484375}, {"start": 840.38, "end": 840.88, "word": " مطلوب", "probability": 0.75341796875}, {"start": 840.88, "end": 841.22, "word": " أنها", "probability": 0.54296875}, {"start": 841.22, "end": 841.76, "word": " نفسها", "probability": 0.7684733072916666}], "temperature": 1.0}, {"id": 36, "seek": 86267, "start": 842.85, "end": 862.67, "text": "بعد هيك بيحكيني إنه إذا كانت charged and polar surfaced، إذا كان الحمق للبروتينة بتكون من أحماض أمينية مشفونة و polar، قطبية، إذن القطبية هيتفاعل مع جزيئات الماء، مع ال solvent، interact with the solvent and increase solubility وهتزود الذائبية، وهيكون ذائب", "tokens": [3555, 22488, 39896, 4117, 4724, 1829, 5016, 4117, 1829, 22653, 36145, 3224, 11933, 15730, 25961, 2655, 1290, 70, 292, 293, 12367, 9684, 3839, 12399, 11933, 15730, 25961, 21542, 2304, 4587, 24976, 26890, 35473, 9957, 3660, 39894, 30544, 9154, 5551, 35571, 46958, 5551, 2304, 9957, 10632, 37893, 5172, 11536, 3660, 4032, 12367, 12399, 12174, 9566, 3555, 10632, 12399, 11933, 8848, 1863, 25062, 9566, 3555, 10632, 39896, 2655, 5172, 995, 30241, 20449, 10874, 11622, 1829, 19986, 9307, 9673, 16606, 12399, 20449, 2423, 33575, 12399, 4648, 365, 264, 33575, 293, 3488, 1404, 836, 1140, 37037, 2655, 11622, 23328, 32545, 16373, 3555, 10632, 12399, 37037, 1829, 30544, 29910, 16373, 3555], "avg_logprob": -0.2178738345609647, "compression_ratio": 1.6040816326530611, "no_speech_prob": 2.6226043701171875e-06, "words": [{"start": 842.85, "end": 843.21, "word": "بعد", "probability": 0.900390625}, {"start": 843.21, "end": 843.35, "word": " هيك", "probability": 0.6824951171875}, {"start": 843.35, "end": 843.87, "word": " بيحكيني", "probability": 0.8824869791666666}, {"start": 843.87, "end": 844.15, "word": " إنه", "probability": 0.6832275390625}, {"start": 844.15, "end": 844.31, "word": " إذا", "probability": 0.94287109375}, {"start": 844.31, "end": 844.71, "word": " كانت", "probability": 0.899658203125}, {"start": 844.71, "end": 845.33, "word": " charged", "probability": 0.6600748697916666}, {"start": 845.33, "end": 845.59, "word": " and", "probability": 0.69970703125}, {"start": 845.59, "end": 845.97, "word": " polar", "probability": 0.7685546875}, {"start": 845.97, "end": 847.01, "word": " surfaced،", "probability": 0.5819498697916666}, {"start": 847.01, "end": 847.39, "word": " إذا", "probability": 0.975341796875}, {"start": 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854.09, "word": " القطبية", "probability": 0.8211669921875}, {"start": 854.09, "end": 854.61, "word": " هيتفاعل", "probability": 0.84638671875}, {"start": 854.61, "end": 854.77, "word": " مع", "probability": 0.994140625}, {"start": 854.77, "end": 855.25, "word": " جزيئات", "probability": 0.91552734375}, {"start": 855.25, "end": 855.65, "word": " الماء،", "probability": 0.8859049479166666}, {"start": 855.65, "end": 855.75, "word": " مع", "probability": 0.98095703125}, {"start": 855.75, "end": 855.89, "word": " ال", "probability": 0.92431640625}, {"start": 855.89, "end": 856.45, "word": " solvent،", "probability": 0.843994140625}, {"start": 856.45, "end": 856.75, "word": " interact", "probability": 0.7216796875}, {"start": 856.75, "end": 857.23, "word": " with", "probability": 0.947265625}, {"start": 857.23, "end": 857.43, "word": " the", "probability": 0.88037109375}, {"start": 857.43, "end": 857.91, "word": " solvent", "probability": 0.91259765625}, {"start": 857.91, "end": 858.45, "word": " and", "probability": 0.72216796875}, {"start": 858.45, "end": 858.99, "word": " increase", "probability": 0.869140625}, {"start": 858.99, "end": 859.59, "word": " solubility", "probability": 0.7200113932291666}, {"start": 859.59, "end": 860.31, "word": " وهتزود", "probability": 0.740234375}, {"start": 860.31, "end": 861.37, "word": " الذائبية،", "probability": 0.8810546875}, {"start": 861.37, "end": 861.87, "word": " وهيكون", "probability": 0.74365234375}, {"start": 861.87, "end": 862.67, "word": " ذائب", "probability": 0.9552408854166666}], "temperature": 1.0}, {"id": 37, "seek": 88905, "start": 863.39, "end": 889.05, "text": "إيش هي الـ net charge of البروتين؟ إيش تخريف الـ net charge المحصلة النهائية للشحنات؟ هي average of all charge هي محصلة كل الشحنات، بجمع كل الشحنات مع بعضها، بشوف الشحنة النهائية at a certain pH بروتين نت charge والـ P0، this is called the isoelectric point حكينا إن الـ isoelectric في نقطة اسمها الـ isoelectric point، وهي قيمة pH", "tokens": [28814, 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"word": "إيش", "probability": 0.6795654296875}, {"start": 863.71, "end": 863.89, "word": " هي", "probability": 0.84619140625}, {"start": 863.89, "end": 864.01, "word": " الـ", "probability": 0.66552734375}, {"start": 864.01, "end": 864.11, "word": " net", "probability": 0.740234375}, {"start": 864.11, "end": 864.49, "word": " charge", "probability": 0.966796875}, {"start": 864.49, "end": 864.71, "word": " of", "probability": 0.93505859375}, {"start": 864.71, "end": 865.25, "word": " البروتين؟", "probability": 0.82080078125}, {"start": 865.25, "end": 865.33, "word": " إيش", "probability": 0.8310546875}, {"start": 865.33, "end": 865.73, "word": " تخريف", "probability": 0.50640869140625}, {"start": 865.73, "end": 865.87, "word": " الـ", "probability": 0.735595703125}, {"start": 865.87, "end": 865.99, "word": " net", "probability": 0.9345703125}, {"start": 865.99, "end": 866.35, "word": " charge", "probability": 0.97119140625}, {"start": 866.35, "end": 866.97, "word": " المحصلة", "probability": 0.7625732421875}, {"start": 866.97, "end": 867.55, "word": " النهائية", "probability": 0.99267578125}, {"start": 867.55, "end": 868.49, "word": " للشحنات؟", "probability": 0.8856608072916666}, {"start": 868.49, "end": 868.85, "word": " هي", "probability": 0.689453125}, {"start": 868.85, "end": 869.31, "word": " average", "probability": 0.7890625}, {"start": 869.31, "end": 869.61, "word": " of", "probability": 0.98486328125}, {"start": 869.61, "end": 869.99, "word": " all", "probability": 0.95751953125}, {"start": 869.99, "end": 870.55, "word": " charge", "probability": 0.56640625}, {"start": 870.55, "end": 870.79, "word": " هي", "probability": 0.6611328125}, {"start": 870.79, "end": 871.55, "word": " محصلة", "probability": 0.941650390625}, {"start": 871.55, "end": 871.95, "word": " كل", "probability": 0.98046875}, {"start": 871.95, "end": 872.61, "word": " الشحنات،", "probability": 0.876611328125}, {"start": 872.61, "end": 872.91, "word": " بجمع", "probability": 0.877197265625}, {"start": 872.91, "end": 873.11, "word": " كل", "probability": 0.9853515625}, {"start": 873.11, "end": 873.55, "word": " الشحنات", "probability": 0.989501953125}, {"start": 873.55, "end": 873.73, "word": " مع", "probability": 0.99658203125}, {"start": 873.73, "end": 874.57, "word": " بعضها،", "probability": 0.8924560546875}, {"start": 874.57, "end": 874.87, "word": " بشوف", "probability": 0.9451497395833334}, {"start": 874.87, "end": 875.45, "word": " الشحنة", "probability": 0.95849609375}, {"start": 875.45, "end": 876.43, "word": " النهائية", "probability": 0.9906005859375}, {"start": 876.43, "end": 877.09, "word": " at", "probability": 0.7177734375}, {"start": 877.09, "end": 877.31, "word": " a", "probability": 0.97607421875}, {"start": 877.31, "end": 877.77, "word": " certain", "probability": 0.916015625}, {"start": 877.77, "end": 878.45, "word": " pH", "probability": 0.88037109375}, {"start": 878.45, "end": 879.37, "word": " بروتين", "probability": 0.77679443359375}, {"start": 879.37, "end": 879.61, "word": " نت", "probability": 0.6702880859375}, {"start": 879.61, "end": 879.99, "word": " charge", "probability": 0.5400390625}, {"start": 879.99, "end": 880.27, "word": " والـ", "probability": 0.680908203125}, {"start": 880.27, "end": 881.25, "word": " P0،", "probability": 0.5555013020833334}, {"start": 881.25, "end": 881.43, "word": " this", "probability": 0.88330078125}, {"start": 881.43, "end": 881.65, "word": " is", "probability": 0.96484375}, {"start": 881.65, "end": 882.19, "word": " called", "probability": 0.92138671875}, {"start": 882.19, "end": 882.61, "word": " the", "probability": 0.77392578125}, {"start": 882.61, "end": 883.25, "word": " isoelectric", "probability": 0.8401692708333334}, {"start": 883.25, "end": 883.75, "word": " point", "probability": 0.68603515625}, {"start": 883.75, "end": 884.43, "word": " حكينا", "probability": 0.8870849609375}, {"start": 884.43, "end": 884.57, "word": " إن", "probability": 0.7431640625}, {"start": 884.57, "end": 884.65, "word": " الـ", "probability": 0.20654296875}, {"start": 884.65, "end": 885.11, "word": " isoelectric", "probability": 0.779296875}, {"start": 885.11, "end": 885.31, "word": " في", "probability": 0.64697265625}, {"start": 885.31, "end": 885.77, "word": " نقطة", "probability": 0.7943522135416666}, {"start": 885.77, "end": 886.23, "word": " اسمها", "probability": 0.9650065104166666}, {"start": 886.23, "end": 886.41, "word": " الـ", "probability": 0.863037109375}, {"start": 886.41, "end": 887.05, "word": " isoelectric", "probability": 0.93310546875}, {"start": 887.05, "end": 887.79, "word": " point،", "probability": 0.756591796875}, {"start": 887.79, "end": 888.25, "word": " وهي", "probability": 0.834716796875}, {"start": 888.25, "end": 888.75, "word": " قيمة", "probability": 0.9957682291666666}, {"start": 888.75, "end": 889.05, "word": " pH", "probability": 0.5712890625}], "temperature": 1.0}, {"id": 38, "seek": 89501, "start": 889.51, "end": 895.01, "text": "اللي عندها بيكون ال mid charge بتساوي zero الشحنة الكلية بتساوي صفر", "tokens": [6027, 20292, 43242, 11296, 4724, 1829, 30544, 2423, 2062, 4602, 39894, 3794, 995, 45865, 4018, 25124, 5016, 1863, 3660, 33251, 1211, 10632, 39894, 3794, 995, 45865, 20328, 5172, 2288], "avg_logprob": -0.24518230458100637, "compression_ratio": 1.1578947368421053, "no_speech_prob": 0.0, "words": [{"start": 889.51, "end": 889.83, "word": "اللي", "probability": 0.82421875}, {"start": 889.83, "end": 890.17, "word": " عندها", "probability": 0.9892578125}, {"start": 890.17, "end": 890.57, "word": " بيكون", "probability": 0.89501953125}, {"start": 890.57, "end": 890.75, "word": " ال", "probability": 0.84765625}, {"start": 890.75, "end": 890.99, "word": " mid", "probability": 0.1666259765625}, {"start": 890.99, "end": 891.59, "word": " charge", "probability": 0.8515625}, {"start": 891.59, "end": 892.39, "word": " بتساوي", "probability": 0.87548828125}, {"start": 892.39, "end": 892.71, "word": " zero", "probability": 0.6025390625}, {"start": 892.71, "end": 893.19, "word": " الشحنة", "probability": 0.841552734375}, {"start": 893.19, "end": 893.75, "word": " الكلية", "probability": 0.7279459635416666}, {"start": 893.75, "end": 894.49, "word": " بتساوي", "probability": 0.967041015625}, {"start": 894.49, "end": 895.01, "word": " صفر", "probability": 0.6901041666666666}], "temperature": 1.0}], "language": "ar", "language_probability": 1.0, "duration": 899.2391875, "duration_after_vad": 867.9806250000005}
\ No newline at end of file
diff --git a/PL9fwy3NUQKwZ1Q7zOg1ObgufPMjRI3WY1/qvn-U_MgMmU.srt b/PL9fwy3NUQKwZ1Q7zOg1ObgufPMjRI3WY1/qvn-U_MgMmU.srt
new file mode 100644
index 0000000000000000000000000000000000000000..1c581f5e921ad7b60992eddea96491995c3ede80
--- /dev/null
+++ b/PL9fwy3NUQKwZ1Q7zOg1ObgufPMjRI3WY1/qvn-U_MgMmU.srt
@@ -0,0 +1,794 @@
+1
+00:00:00,000 --> 00:00:03,860
+At the active site هذا كيف بيثبت الـ non
+
+2
+00:00:03,860 --> 00:00:07,100
+-competitive؟ الـ non-competitive بيجي بيرتبط في 
+
+3
+00:00:07,100 --> 00:00:11,560
+موقع اسمه الـ allosteric site موجود في الإنزيم تمام؟
+
+4
+00:00:12,150 --> 00:00:16,930
+مجرد ما يرتبط الـ Non-competitive Inhibitor بيعمل تغيير
+
+5
+00:00:16,930 --> 00:00:21,110
+وتحوير لشكل الـ Active Site بيغير شكل الـ Active
+
+6
+00:00:21,110 --> 00:00:24,790
+Site فبيبطل الـ Active Site شكله ملائم للـ
+
+7
+00:00:24,790 --> 00:00:27,930
+Substrate بتيجي الـ Substrate ده ترتبط بتلاقي
+
+8
+00:00:27,930 --> 00:00:32,250
+الموقع مختلف الـ Active Site مش ملائم لها، غير 
+
+9
+00:00:32,250 --> 00:00:36,850
+ملائم للارتباط فبتقدرش ترتبط فبيثبت ارتباط الـ
+
+10
+00:00:36,850 --> 00:00:41,550
+«substrate» مع الـ «enzyme» في موقع الـ «active 
+
+11
+00:00:41,550 --> 00:00:46,410
+site» من خلال تغيير شكل الـ «active site» فبيغير
+
+12
+00:00:46,410 --> 00:00:49,150
+شكل الـ «active site» وبيدّرش يرتبط فيها الـ
+
+13
+00:00:49,150 --> 00:00:51,650
+«substrate» طب هل أنا لو زودت تركيز الـ
+
+14
+00:00:51,650 --> 00:00:55,050
+«substrate» هدي تنحل المشكلة؟ لأ، بتنحلش لأن
+
+15
+00:00:55,050 --> 00:01:00,190
+المشكلة أساسها ارتباطه في موقع آخر وتغيير شكل الـ
+
+16
+00:01:00,190 --> 00:01:01,690
+«active site»
+
+17
+00:01:05,860 --> 00:01:09,660
+الكلمة «KM» لم تتغير «KM» اللي بتعبر عن تركيز
+
+18
+00:01:09,660 --> 00:01:14,100
+السبسترات في حالة non-competitive inhibitor ما
+
+19
+00:01:14,100 --> 00:01:17,700
+بتتغير، ليش؟  هاي non-competitive inhibitor don't
+
+20
+00:01:17,700 --> 00:01:20,660
+interfere with the binding of substrate to enzyme
+
+21
+00:01:20,660 --> 00:01:26,980
+ما بتأثر على ارتباط السبسترات مع الإنزيم لأن الإنزيم
+
+22
+00:01:26,980 --> 00:01:30,620
+يظهر نفس الـKM في الـpresence أو عدم وجود
+
+23
+00:01:30,620 --> 00:01:34,580
+نانكومبيتيف انهيبتور بيحكي إن في حال وجود 
+
+24
+00:01:34,580 --> 00:01:39,580
+نانكومبيتيف انهيبتور أو عدم وجوده، KM نفسها، KM مش
+
+25
+00:01:39,580 --> 00:01:42,380
+هتتغير، لأنه أنا مهما أزود مش زي الأولى
+
+26
+00:01:42,380 --> 00:01:45,400
+الـcompetitive inhibitor، مش هنحل المشكلة، حيث أنا
+
+27
+00:01:45,400 --> 00:01:49,260
+بنزود تركيز السبستريات، فبتغير KM، بتزيد، لكن في
+
+28
+00:01:49,260 --> 00:01:52,300
+هذه الحالة أنا ما فيش داعي أزود أصلا تركيز
+
+29
+00:01:52,300 --> 00:01:55,920
+السبستريات لأن أنا مش هتأثر، مش هتحل المشكلة فـ KM
+
+30
+00:01:55,920 --> 00:02:02,140
+نفسها Vmax بتقل هلجيت أنا لو ما فيش عندي inhibitor
+
+31
+00:02:02,140 --> 00:02:05,200
+هاصل لـ Vmax لكن لو في عندي non-competitive
+
+32
+00:02:05,200 --> 00:02:08,760
+inhibitor خلاص شوّه شكل الـ active site مش هتقدر
+
+33
+00:02:08,760 --> 00:02:12,960
+ترتبط الـ substrate فانا مش هاصل لـ Vmax فVmax في
+
+34
+00:02:12,960 --> 00:02:16,520
+حالة non-competitive inhibitor هتقل increasing
+
+35
+00:02:16,520 --> 00:02:19,380
+the concentration of substrate doesn't overcome
+
+36
+00:02:19,380 --> 00:02:23,640
+non-competitive inhibitorNon-competitive inhibitor
+
+37
+00:02:23,640 --> 00:02:26,480
+decreases the Vmax of the reaction الـ Non
+
+38
+00:02:26,480 --> 00:02:30,600
+-competitive inhibitor بيقلل الـ Vmax Non
+
+39
+00:02:30,600 --> 00:02:33,680
+-competitive inhibitor therefore simply reduces
+
+40
+00:02:33,680 --> 00:02:37,880
+the amount of active enzyme بيقلل كمية الإنزيم
+
+41
+00:02:37,880 --> 00:02:41,560
+النشطة اللي هو موقع الـ Active enzyme فهيقلل الـ
+
+42
+00:02:41,560 --> 00:02:49,490
+Vmax ولكن مش هيؤثر على KM هنا مخطط بيوضح في حال
+
+43
+00:02:49,490 --> 00:02:53,150
+وجود non-inhibitor أو non-competitive inhibitor
+
+44
+00:02:53,150 --> 00:02:56,210
+إذا ما في حالة وجود أو عدم وجود inhibitor في حالة
+
+45
+00:02:56,210 --> 00:02:59,990
+وجود non-competitive inhibitor في حالة عدم وجود
+
+46
+00:02:59,990 --> 00:03:03,710
+inhibitor القاعدة المعروفة اللي حكينا عنها إنه كل
+
+47
+00:03:03,710 --> 00:03:07,330
+ما زاد تركيز الـsubstrate بتزيد سرعة التفاعل
+
+48
+00:03:07,330 --> 00:03:12,330
+الكيميائي ونشاط الإنزيم وصولًا إلى نقطة التشبع
+
+49
+00:03:12,330 --> 00:03:16,250
+للـSaturation Point اللي هي عندها Vmax يثبت
+
+50
+00:03:16,250 --> 00:03:19,970
+المنحنى وصولًا إلى Vmax طيب، في حال وجود Non
+
+51
+00:03:19,970 --> 00:03:24,350
+-competitive inhibitor لكن هذا بيأثر على الـActive
+
+52
+00:03:24,350 --> 00:03:28,110
+site فما بتقدر ترتبط الـSubstrate في الـActive
+
+53
+00:03:28,110 --> 00:03:35,030
+site فهيقل الـ Vmax هل هتتأثر KM هتتأثر تركيز الـ
+
+54
+00:03:35,030 --> 00:03:39,630
+Substrate لأ نفسها وإنما مين اللي هتقل الـ Vmax
+
+55
+00:03:39,630 --> 00:03:43,330
+اللي هي سرعة التفاعل أقصى سرعة لأن أنا مش هقدر 
+
+56
+00:03:43,330 --> 00:03:46,130
+أصلًا اللي هتقل Vmax
+
+57
+00:03:47,790 --> 00:03:50,750
+في النهاية الملخص «competitive inhibitor» عندنا 
+
+58
+00:03:50,750 --> 00:03:53,070
+أنواع الـ «inhibitor» «competitive» و «non
+
+59
+00:03:53,070 --> 00:03:55,610
+-competitive» في حالة الـ «competitive»، إيش
+
+60
+00:03:55,610 --> 00:04:00,010
+هيتغير؟ «KM» بتزيد، تركيز الـ «substrate» بيزيد أما
+
+61
+00:04:00,010 --> 00:04:04,070
+«Vmax» ما بتتغير، أقصى سرعة التفاعل هي نفسها «non
+
+62
+00:04:04,070 --> 00:04:08,430
+-competitive inhibitor» «KM» ما بتتغير، no change،
+
+63
+00:04:08,430 --> 00:04:15,040
+بتقل «Vmax» بتقلّ، لكن أقصى سرعة التفاعل بتقل بعض
+
+64
+00:04:15,040 --> 00:04:18,500
+المصطلحات الأغلبية حكينا عنها أولًا الـ Active
+
+65
+00:04:18,500 --> 00:04:22,580
+site الـ Active site حكينا هو موقع في الإنزيم
+
+66
+00:04:22,580 --> 00:04:26,920
+بيرتبط فيه الـ substrate لحدوث التفاعل region of
+
+67
+00:04:26,920 --> 00:04:30,340
+enzyme molecule find the substrate and carry the
+
+68
+00:04:30,340 --> 00:04:33,920
+catalytic reaction موقع في الإنزيم بيرتبط فيه الـ
+
+69
+00:04:33,920 --> 00:04:37,540
+substrate لحدوث التفاعل فبالإنزيم، حكينا أنا تعرفي 
+
+70
+00:04:37,540 --> 00:04:41,540
+الإنزيم هو محفز بيولوجي غالباً بيكون globular
+
+71
+00:04:41,540 --> 00:04:45,760
+protein عبارة عن البروتين بينتج من أجسام الكائنات 
+
+72
+00:04:45,760 --> 00:04:50,640
+الحية لتسريع التفاعل الكيميائي وبيطلع بعد التفاعل 
+
+73
+00:04:50,640 --> 00:04:56,520
+كما هو، بدون حدوث أي تدمير أو تغيير على شكله طيب،
+
+74
+00:04:56,520 --> 00:05:00,100
+«KM»، «KM» برضه حكينا هنا إنه هي عبارة عن تركيز
+
+75
+00:05:00,100 --> 00:05:03,920
+الـsubstrate عند نص Vmax عند الـhalf maximum
+
+76
+00:05:03,920 --> 00:05:07,540
+velocity substrate concentration at half maximum
+
+77
+00:05:07,540 --> 00:05:12,620
+velocity «Vmax» حكينا إنه هي أقصى سرعة للتفاعل
+
+78
+00:05:12,620 --> 00:05:17,240
+«Maximum initial velocity of enzyme reaction» أقصى
+
+79
+00:05:17,240 --> 00:05:21,680
+سرعة لمعدل حدود التفاعل الكيميائي وعمل الإنزيم
+
+80
+00:05:21,680 --> 00:05:26,720
+عندها بموصل إلى نقطة الـ «saturation point» إنه
+
+81
+00:05:26,720 --> 00:05:30,680
+بيصير التفاعل constant ثابت بيصير خلاص بيثبت 
+
+82
+00:05:30,680 --> 00:05:34,740
+التفاعل لأنه عندنا «saturating substrate level»
+
+83
+00:05:34,740 --> 00:05:39,530
+عنده نقطة تشبع من السبسترات الـ catalyst اللي هو 
+
+84
+00:05:39,530 --> 00:05:45,570
+المحفز وأي مادة بتسرّع حدوث التفاعل البطيء anything
+
+85
+00:05:45,570 --> 00:05:47,930
+that speeds up a chemical reaction that is
+
+86
+00:05:47,930 --> 00:05:52,890
+occurring slowly أي مادة بتسرّع حدوث التفاعل اللي
+
+87
+00:05:52,890 --> 00:05:57,990
+كان يمشي ببطء بسميها catalyst محفز اليوم تطبيقنا 
+
+88
+00:05:57,990 --> 00:06:01,030
+العمل هيكون على إنزيم اسمه alkaline phosphate
+
+89
+00:06:04,010 --> 00:06:07,170
+الـ alkaline phosphatase هذا بيعمل على تكسير فوسفات
+
+90
+00:06:07,170 --> 00:06:10,030
+جروب بيشتغل على المادة اللي بتحتوي على مجموعة
+
+91
+00:06:10,030 --> 00:06:13,710
+فوسفات بيكسر هذه الفوسفات إذا member of
+
+92
+00:06:13,710 --> 00:06:17,390
+phosphomonoesterase group هو أحد أعضاء مجموعة 
+
+93
+00:06:17,390 --> 00:06:22,180
+إنزيمات يطلق عليها phosphomonoesterase group They
+
+94
+00:06:22,180 --> 00:06:25,880
+are highly specific and act on only one substrate
+
+95
+00:06:25,880 --> 00:06:31,300
+ومتخصص هيشتغل بس على substrates معين حكينا كل 
+
+96
+00:06:31,300 --> 00:06:34,620
+إنزيم متخصص بـ substrate معين وهذا لقناع
+
+97
+00:06:34,620 --> 00:06:38,680
+الفسفاتيز متخصص بـ substrate معينهذه الـ
+
+98
+00:06:38,680 --> 00:06:42,100
+Substrate اللي بتحتوي على فوسفات جروب «الكالاين 
+
+99
+00:06:42,100 --> 00:06:46,180
+فوسفاتيز» لديه فوسفات كبير بشكل خاص بـ pNP عنده 
+
+100
+00:06:46,180 --> 00:06:49,960
+Range واسع من خصوصيته على الـ Substrate كيف يعني؟
+
+101
+00:06:49,960 --> 00:06:53,560
+هو متخصص بـ Substrate واحد لكن هذا الـ Substrate
+
+102
+00:06:53,560 --> 00:06:56,960
+اللي هو متخصص فيه يحتوي على فوسفات جروب ففي أكثر
+
+103
+00:06:56,960 --> 00:07:00,560
+من مادة في الجسم في أكثر من مكان بتحتوي على فوسفات 
+
+104
+00:07:00,560 --> 00:07:05,690
+جروب عشان هيك هو له Broad Range واسع It is named
+
+105
+00:07:05,690 --> 00:07:08,390
+alkaline phosphatase ليش بيسموها alkaline
+
+106
+00:07:08,390 --> 00:07:14,070
+phosphatase؟ لأنه بيشتغل عن pH9، pH alkaline في 
+
+107
+00:07:14,070 --> 00:07:18,490
+عنده مجموعة أخرى بتشتغل عن pH7 بيسموها acid
+
+108
+00:07:18,490 --> 00:07:24,060
+phosphatase، بتشتغل عن pH7 بيتعمل دراسة للـ
+
+109
+00:07:24,060 --> 00:07:27,620
+«Alkaline Phosphatase in Vitro» ندرست أو نحدد الـ
+
+110
+00:07:27,620 --> 00:07:30,380
+«Alkaline Phosphatase» في المعمل «Alkaline
+
+111
+00:07:30,380 --> 00:07:33,680
+Phosphatase activity by following the rate of
+
+112
+00:07:33,680 --> 00:07:36,300
+formation of a product» كيف بتتبر «Alkaline
+
+113
+00:07:36,300 --> 00:07:39,740
+Phosphatase»؟ من خلال متابعة تكون الـ product،
+
+114
+00:07:39,740 --> 00:07:43,040
+النواتج، إني بدي أجيب الإنزيم هاد أحطله الـ
+
+115
+00:07:43,040 --> 00:07:46,700
+substrate المتخصص فيه يرتبطوا مع بعض ويحولوا إلى
+
+116
+00:07:46,700 --> 00:07:50,960
+product أشوف هذا ال product الناتج من خلالها بدّرس
+
+117
+00:07:50,960 --> 00:07:54,450
+اللي هو هذا الإنزيم الـ Alkaline Phosphatase اللي
+
+118
+00:07:54,450 --> 00:07:57,730
+بيشتغل على حكاية هنا الـ substrate تحتوي على فوسفات
+
+119
+00:07:57,730 --> 00:08:02,570
+grow على alkaline media على pH alkaline بتكون بينجل
+
+120
+00:08:02,570 --> 00:08:05,910
+مجموعة الفسفات ي grow فأنا بدي أحطله اليوم
+
+121
+00:08:05,910 --> 00:08:10,770
+substrate هو 4-nitrophenyl phosphate تمام؟ وأحطله 
+
+122
+00:08:10,770 --> 00:08:15,310
+alkaline pH وبيشتغل عليها ويحولني إياها الـ 4
+
+123
+00:08:15,310 --> 00:08:19,720
+-nitrophenol هذه الـ 4-nitrophenol بقراها على جهاز
+
+124
+00:08:19,720 --> 00:08:25,300
+Spectrophotometer على طول موجي 400 نانومتر بقراها
+
+125
+00:08:25,300 --> 00:08:29,040
+بجيب كمية الضوء اللي امتصتها العين لأن لونها بتكون
+
+126
+00:08:29,040 --> 00:08:33,660
+bright yellow ولكن باقي المكونات والنواتج ما بتكون
+
+127
+00:08:33,660 --> 00:08:37,540
+لونها بتكون colorless فانا بدي أقرا بس للـ 4
+
+128
+00:08:37,540 --> 00:08:42,160
+-nitrophenol فبشوف من خلال هذه القراءات بعرف نشاط
+
+129
+00:08:42,160 --> 00:08:46,610
+الإنزيم إذا أنا عندي إنزيم ألكالينفوسفاتيز بحطله
+
+130
+00:08:46,610 --> 00:08:50,470
+substrate وتتكون عندي product زي المعادلة اللي 
+
+131
+00:08:50,470 --> 00:08:53,790
+حكيناها أولًا بإنزيم زائد substrate بيعطيني
+
+132
+00:08:53,790 --> 00:08:57,050
+إنزيم substrate complex وبعدين بيبتدي عندي إنزيم 
+
+133
+00:08:57,050 --> 00:09:00,210
+و product مين أنا عند الإنزيم والsubstrate؟
+
+134
+00:09:00,210 --> 00:09:03,670
+الإنزيم هو ألكالينفوسفاتيز زائد substrate 4
+
+135
+00:09:03,670 --> 00:09:07,530
+-nitrophenyl phosphate إيش الناتج بدي يطلع عندي؟ بدي 
+
+136
+00:09:07,530 --> 00:09:10,270
+يطلع عندي إنزيم و product الإنزيم هو
+
+137
+00:09:10,270 --> 00:09:14,010
+للكلينفوسفاتيز، هيطلع زي ما هو زائد مجموعة الـ
+
+138
+00:09:14,010 --> 00:09:18,430
+Phosphate Group بيكسرها هذا الإنزيم، زائد 4
+
+139
+00:09:18,430 --> 00:09:22,190
+-nitrophenol. الـ 4-nitrophenol هذه اللي هي yellow
+
+140
+00:09:22,190 --> 00:09:25,630
+color، لونها أصفر، بقرأ الـ absorption، read
+
+141
+00:09:25,630 --> 00:09:30,310
+absorption، لها 400 نانومتر، بقرأ لها الامتصاص على
+
+142
+00:09:30,310 --> 00:09:36,560
+400 نانومتر، طول موجي 400. كيف بدأ أجيز نسمة الـ
+
+143
+00:09:36,560 --> 00:09:39,360
+«measurement of alkaline phosphatase activity»
+
+144
+00:09:39,360 --> 00:09:42,080
+بناء على مادة الـ«substrate» اللي هي هذه
+
+145
+00:09:42,080 --> 00:09:45,260
+الـ«artificial» المصنعة هي الـ«نيتروفينيل فوسفات»
+
+146
+00:09:45,260 --> 00:09:47,560
+هذا الـ«substrate» بيرتبط مع الـ«alkaline
+
+147
+00:09:47,560 --> 00:09:50,520
+phosphatase» وبيعطينا product حكينا الـ«product»
+
+148
+00:09:50,520 --> 00:09:55,430
+ينتج لون yellow بيحكي لي إن شدة اللون، intensity of
+
+149
+00:09:55,430 --> 00:09:58,930
+yellow color شدة اللون هذا الأصفر بتعبّر لي عن 
+
+150
+00:09:58,930 --> 00:10:02,130
+degree to which enzyme has acted upon a substrate
+
+151
+00:10:02,130 --> 00:10:07,430
+عن درجة ارتباط الإنزيم مع الـ substrate، درجة عمل
+
+152
+00:10:07,430 --> 00:10:12,590
+الإنزيم شدة اللون تعبّر على درجة عمل الإنزيم
+
+153
+00:10:14,750 --> 00:10:17,630
+في عندنا هنا تعريف الـ enzyme kinetic وهذا حكينا
+
+154
+00:10:17,630 --> 00:10:20,490
+عنه في الأول أنه ي study في rate at which enzyme
+
+155
+00:10:20,490 --> 00:10:24,690
+works دراسة معدل حدوث التفاعل طب أنا كيف بدي أدرس
+
+156
+00:10:24,690 --> 00:10:27,770
+هنا معدل حدوث التفاعل؟ اليوم الدراسة بتكون على
+
+157
+00:10:27,770 --> 00:10:31,630
+إنزيم الألقالي الفوسفاتين بدي أدرس معدل حدوث
+
+158
+00:10:31,630 --> 00:10:35,570
+الـAlkaline Phosphatase بناءً على إيش؟ اليوم بدي
+
+159
+00:10:35,570 --> 00:10:38,850
+أدرس بناءً على تغيير في تركيز الـsubstrate يعني
+
+160
+00:10:38,850 --> 00:10:43,830
+أنا في نهاية المعمل بعد شغلي بدي أشوف العلاقة ما
+
+161
+00:10:43,830 --> 00:10:47,860
+بين تركيز الـsubstrate وسرعة التفاعل الكيميائي أحنا
+
+162
+00:10:47,860 --> 00:10:52,040
+حكينا إن هذه العلاقة خطية وصولًا إلى Vmax إلى نقطة
+
+163
+00:10:52,040 --> 00:10:55,320
+التشبع اللي هي الـ saturation point بتبطل العلاقة 
+
+164
+00:10:55,320 --> 00:10:58,460
+خطية وإنما بتثبت فأنا اليوم بدي أدرس هذه العلاقة
+
+165
+00:10:58,460 --> 00:11:03,880
+كيف بدي أدرسها؟  بحكي لي إن نجيب test tubes ونحط فيها
+
+166
+00:11:03,880 --> 00:11:07,340
+تركيز مختلفة من الـ substrates يعني مثلًا الأولى
+
+167
+00:11:07,340 --> 00:11:12,560
+بدي أحط فيها تركيز substrates خمسة الثانية تركيز
+
+168
+00:11:12,560 --> 00:11:15,160
+الـ substrates يكون فيها عشرة واللي بعدها تركيز
+
+169
+00:11:15,160 --> 00:11:19,160
+الـ substrates مثلًا خمسة عشر بعدها عشرين بعدها خمسة
+
+170
+00:11:19,160 --> 00:11:23,140
+وعشرين بعدها بدي أحط تلاتين خمسة وثلاثين وهكذا 
+
+171
+00:11:23,140 --> 00:11:26,320
+كل tube ده تكون تركيز الـ substrates فيها أعلى من
+
+172
+00:11:26,320 --> 00:11:29,770
+الـ tube اللي قبلها صار عندي tubes فيها Substrate
+
+173
+00:11:29,770 --> 00:11:34,250
+بتركيز معين بدي هجيب الـ Enzyme عشان يرتبط مع الـ
+
+174
+00:11:34,250 --> 00:11:37,330
+Substrate ويكوّن لي Product هلجيت بحط على الـ Tube
+
+175
+00:11:37,330 --> 00:11:39,950
+الأول كلهم نفس الكمية من الـ Enzyme بدي أحط عليها
+
+176
+00:11:39,950 --> 00:11:43,230
+Enzyme يجي الـ Enzyme بيرتبط مع الـ Substrate في
+
+177
+00:11:43,230 --> 00:11:46,510
+الـ Tube الأول وبيعطيني Product بدي أقرأه في جهاز
+
+178
+00:11:46,51
+
+201
+00:13:10,070 --> 00:13:13,750
+بعد ما أشتغل شغلي وبعد ما أحط التيوبات، أحط فيها 
+
+202
+00:13:13,750 --> 00:13:17,290
+تركيز مختلف من الـ Substrate أضيف عليها Enzyme،
+
+203
+00:13:17,290 --> 00:13:20,410
+كمية Enzyme، يتفاعلوا مع بعض ويعطيني Product
+
+204
+00:13:20,410 --> 00:13:24,130
+أقراها على جهاز الـ Spectrum، تطلع عندي Absorbance
+
+205
+00:13:24,130 --> 00:13:26,830
+صار عندي معروف Concentration للـ Substrate و
+
+206
+00:13:26,830 --> 00:13:31,770
+Absorbance، بدي أرسم مخطط، Standard Curve هل أنت كيف 
+
+207
+00:13:31,770 --> 00:13:35,210
+متوقع أنه يكون شكل المدخول مخطط؟ بناء على العلاقة
+
+208
+00:13:35,210 --> 00:13:37,890
+اللي حكينا عنها اللي هي العلاقة بين الـSubstrate
+
+209
+00:13:37,890 --> 00:13:43,310
+Concentration وسرعة التفاعل أو معدل حدوث التفاعل
+
+210
+00:13:43,310 --> 00:13:47,390
+العلاقة الحكينا عنها الطرضية وصولا إلى Vmax، إلى
+
+211
+00:13:47,390 --> 00:13:51,410
+الـMaximum Velocity فبناء عليها، هشوف النتاج بتظل
+
+212
+00:13:51,410 --> 00:13:56,130
+هتزيد معي وفجأة بتثبت النتاج، هوصل إلى نقطة
+
+213
+00:13:56,130 --> 00:14:01,090
+Saturation Point، إلى ثبات النتاج احنا في النهاية
+
+214
+00:14:01,090 --> 00:14:06,350
+بنرسم هذا المخطط بدي أرسم على الخط الصيني اللي هي
+
+215
+00:14:06,350 --> 00:14:09,890
+نقطة الخط الصيني بدي أرسم عليها الـ Concentration
+
+216
+00:14:09,890 --> 00:14:13,030
+للـ Substrate، الخط الصادي بدي أرسم الـ Absorbance
+
+217
+00:14:13,030 --> 00:14:15,950
+كل نقطة جديش إليها Concentration وجديش إليها
+
+218
+00:14:15,950 --> 00:14:20,170
+Substrate وأرسمها وأرسم اللي يطلع معايا المنحنى
+
+219
+00:14:20,170 --> 00:14:25,230
+هيطلع معايا علاقة طردية وفجأة بيثبت المنحنى متى
+
+220
+00:14:25,230 --> 00:14:31,060
+هيثبت عند وصول إلى Saturation Point، طريقة العمل 
+
+221
+00:14:31,060 --> 00:14:35,340
+بالتفصيل هنحكي عنها لما نشتغلها في المعمل
diff --git a/PL9fwy3NUQKwZ1Q7zOg1ObgufPMjRI3WY1/qvn-U_MgMmU_raw.json b/PL9fwy3NUQKwZ1Q7zOg1ObgufPMjRI3WY1/qvn-U_MgMmU_raw.json
new file mode 100644
index 0000000000000000000000000000000000000000..8d47e948a946cee2bc6fc6eb6874683f2c2bfa8d
--- /dev/null
+++ b/PL9fwy3NUQKwZ1Q7zOg1ObgufPMjRI3WY1/qvn-U_MgMmU_raw.json
@@ -0,0 +1 @@
+{"segments": [{"id": 1, "seek": 1156, "start": 0.0, "end": 11.56, "text": "At the active site هذا كيف بيثبت الـ non-competitive؟ الـ non-competitive بيجي بيرتبط في موقع اسمه الـ steric site موجود في الإنزع تمام؟", "tokens": [18684, 264, 4967, 3621, 23758, 9122, 33911, 4724, 1829, 12984, 3555, 2655, 2423, 39184, 2107, 12, 1112, 7275, 2187, 22807, 2423, 39184, 2107, 12, 1112, 7275, 2187, 4724, 1829, 7435, 1829, 4724, 1829, 43500, 3555, 9566, 8978, 3714, 30543, 3615, 24525, 2304, 3224, 2423, 39184, 18924, 299, 3621, 3714, 29245, 23328, 8978, 33688, 1863, 11622, 3615, 46811, 10943, 22807], "avg_logprob": -0.30885415424903234, "compression_ratio": 1.3541666666666667, "no_speech_prob": 0.0002465248107910156, "words": [{"start": 0.0, "end": 0.56, "word": "At", "probability": 0.06689453125}, {"start": 0.56, "end": 0.98, "word": " the", "probability": 0.78125}, {"start": 0.98, "end": 1.42, "word": " active", "probability": 0.8115234375}, {"start": 1.42, "end": 1.78, "word": " site", 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"end": 469.0, "word": " الناتج", "probability": 0.8885091145833334}, {"start": 469.0, "end": 469.16, "word": " من", "probability": 0.97705078125}, {"start": 469.16, "end": 469.82, "word": " خلالها", "probability": 0.98779296875}, {"start": 469.82, "end": 470.96, "word": " بدرست", "probability": 0.76904296875}, {"start": 470.96, "end": 471.14, "word": " اللي", "probability": 0.9609375}, {"start": 471.14, "end": 471.42, "word": " هو", "probability": 0.978515625}, {"start": 471.42, "end": 471.72, "word": " هذا", "probability": 0.47265625}, {"start": 471.72, "end": 472.22, "word": " اللينزين", "probability": 0.8896484375}], "temperature": 1.0}, {"id": 21, "seek": 49615, "start": 472.87, "end": 496.15, "text": "الـ Alkaline Phosphatase اللي بيشتغل على حكاية هنا الـ substrate تحتوي على فسفات grow على alkaline media على pH alkaline تكون بينجل مجموعة الفسفات ي grow فأنا بدي أحطله اليوم substrate هو أربع نيتروفينال فسفات تمام؟ و أحطله alkaline pH وبيشتغل عليها ويحولني إياها الأربع نيتروفينال", 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" بتثبت", "probability": 0.8355712890625}, {"start": 834.05, "end": 834.95, "word": " النتاج،", "probability": 0.77203369140625}, {"start": 834.95, "end": 835.41, "word": " هوصل", "probability": 0.740478515625}, {"start": 835.41, "end": 835.65, "word": " إلى", "probability": 0.9716796875}, {"start": 835.65, "end": 836.13, "word": " نقطة", "probability": 0.9933268229166666}, {"start": 836.13, "end": 836.97, "word": " Saturation", "probability": 0.759033203125}, {"start": 836.97, "end": 837.51, "word": " Point", "probability": 0.85546875}], "temperature": 1.0}, {"id": 37, "seek": 83892, "start": 837.86, "end": 838.92, "text": "الى ثبات", "tokens": [6027, 7578, 38637, 3555, 9307], "avg_logprob": -0.2249348908662796, "compression_ratio": 0.625, "no_speech_prob": 0.0, "words": [{"start": 837.86, "end": 838.16, "word": "الى", "probability": 0.713623046875}, {"start": 838.16, "end": 838.92, "word": " ثبات", "probability": 0.8372395833333334}], "temperature": 1.0}, {"id": 38, "seek": 86855, "start": 839.01, "end": 868.55, "text": "النتاج احنا في النهاية بنرسم هذا المخطط بدي أرسم على الخط الصيني اللي هي نقطة الخط الصيني بدي أرسم عليها الـ Concentration للـ Substrate الخط الصادى بدي أرسم الـ Absorbance كل نقطة جديش إليها Concentration وجديش إليها Substrate وأرسمها وأرسم اللي يطلع معايا المنحنة هيطلع معايا علاقة ترضية وفجأة بيثبت المنحنة متى هيثبت عند وصول إلى Saturation Point", "tokens": [6027, 29399, 26108, 1975, 5016, 8315, 8978, 28239, 11296, 10632, 44945, 2288, 38251, 23758, 9673, 9778, 9566, 9566, 4724, 16254, 5551, 2288, 38251, 15844, 33962, 9566, 31767, 9957, 1829, 13672, 1829, 39896, 8717, 47432, 3660, 33962, 9566, 31767, 9957, 1829, 4724, 16254, 5551, 2288, 38251, 25894, 11296, 2423, 39184, 2656, 2207, 2405, 24976, 39184, 42090, 4404, 33962, 9566, 31767, 18513, 7578, 4724, 16254, 5551, 2288, 38251, 2423, 39184, 5813, 30907, 719, 28242, 8717, 47432, 3660, 10874, 16254, 8592, 11933, 20292, 11296, 2656, 2207, 2405, 49610, 16254, 8592, 11933, 20292, 11296, 42090, 4404, 36725, 2288, 38251, 11296, 36725, 2288, 38251, 13672, 1829, 7251, 9566, 1211, 3615, 20449, 995, 25528, 9673, 1863, 5016, 1863, 3660, 39896, 9566, 1211, 3615, 20449, 995, 25528, 11203, 995, 28671, 6055, 43042, 10632, 4032, 5172, 7435, 10721, 3660, 4724, 1829, 12984, 3555, 2655, 9673, 1863, 5016, 1863, 3660, 44650, 7578, 39896, 12984, 3555, 2655, 43242, 4032, 9381, 12610, 30731, 5344, 8167, 12387], "avg_logprob": -0.15224359260919768, "compression_ratio": 2.1185185185185187, "no_speech_prob": 0.10296630859375, "words": [{"start": 839.01, "end": 839.85, "word": "النتاج", "probability": 0.8779296875}, {"start": 839.85, "end": 840.37, "word": " احنا", "probability": 0.8743489583333334}, {"start": 840.37, "end": 840.55, "word": " في", "probability": 0.79638671875}, {"start": 840.55, "end": 841.09, "word": " النهاية", "probability": 0.9322916666666666}, {"start": 841.09, "end": 841.75, "word": " بنرسم", "probability": 0.8707682291666666}, {"start": 841.75, "end": 842.03, "word": " هذا", "probability": 0.90966796875}, {"start": 842.03, "end": 842.71, "word": " المخطط", "probability": 0.975341796875}, {"start": 842.71, "end": 843.37, "word": " بدي", "probability": 0.773681640625}, {"start": 843.37, "end": 843.71, "word": " أرسم", "probability": 0.7989095052083334}, {"start": 843.71, "end": 843.95, "word": " على", "probability": 0.87890625}, {"start": 843.95, "end": 844.33, "word": " الخط", "probability": 0.979736328125}, {"start": 844.33, "end": 845.53, "word": " الصيني", "probability": 0.8289388020833334}, {"start": 845.53, "end": 846.01, "word": " اللي", "probability": 0.89794921875}, {"start": 846.01, "end": 846.35, "word": " هي", "probability": 0.8779296875}, {"start": 846.35, "end": 847.17, "word": " نقطة", "probability": 0.81494140625}, {"start": 847.17, "end": 847.89, "word": " الخط", "probability": 0.69384765625}, {"start": 847.89, "end": 848.17, "word": " الصيني", "probability": 0.96484375}, {"start": 848.17, "end": 848.37, "word": " بدي", "probability": 0.92431640625}, {"start": 848.37, "end": 848.69, "word": " أرسم", "probability": 0.9352213541666666}, {"start": 848.69, "end": 849.15, "word": " عليها", "probability": 0.771728515625}, {"start": 849.15, "end": 849.31, "word": " الـ", "probability": 0.85107421875}, {"start": 849.31, "end": 849.89, "word": " Concentration", "probability": 0.7228190104166666}, {"start": 849.89, "end": 850.13, "word": " للـ", "probability": 0.697265625}, {"start": 850.13, "end": 850.63, "word": " Substrate", "probability": 0.619384765625}, {"start": 850.63, "end": 851.25, "word": " الخط", "probability": 0.912353515625}, {"start": 851.25, "end": 851.71, "word": " الصادى", "probability": 0.656982421875}, {"start": 851.71, "end": 851.93, "word": " بدي", "probability": 0.970703125}, {"start": 851.93, "end": 852.19, "word": " أرسم", "probability": 0.9690755208333334}, {"start": 852.19, "end": 852.37, "word": " الـ", "probability": 0.87109375}, {"start": 852.37, "end": 853.03, "word": " Absorbance", "probability": 0.7490234375}, {"start": 853.03, "end": 853.61, "word": " كل", "probability": 0.9267578125}, {"start": 853.61, "end": 853.99, "word": " نقطة", "probability": 0.9884440104166666}, {"start": 853.99, "end": 854.35, "word": " جديش", "probability": 0.8103841145833334}, {"start": 854.35, "end": 854.57, "word": " إليها", "probability": 0.837890625}, {"start": 854.57, "end": 855.27, "word": " Concentration", "probability": 0.823974609375}, {"start": 855.27, "end": 855.69, "word": " وجديش", "probability": 0.8782552083333334}, {"start": 855.69, "end": 855.95, "word": " إليها", "probability": 0.9842122395833334}, {"start": 855.95, "end": 856.57, "word": " Substrate", "probability": 0.94287109375}, {"start": 856.57, "end": 857.29, "word": " وأرسمها", "probability": 0.8665771484375}, {"start": 857.29, "end": 858.21, "word": " وأرسم", "probability": 0.86767578125}, {"start": 858.21, "end": 858.89, "word": " اللي", "probability": 0.94140625}, {"start": 858.89, "end": 859.29, "word": " يطلع", "probability": 0.9732666015625}, {"start": 859.29, "end": 859.59, "word": " معايا", "probability": 0.8260091145833334}, {"start": 859.59, "end": 860.17, "word": " المنحنة", "probability": 0.94833984375}, {"start": 860.17, "end": 860.83, "word": " هيطلع", "probability": 0.90625}, {"start": 860.83, "end": 861.07, "word": " معايا", "probability": 0.9581705729166666}, {"start": 861.07, "end": 861.51, "word": " علاقة", "probability": 0.98046875}, {"start": 861.51, "end": 862.09, "word": " ترضية", "probability": 0.9391276041666666}, {"start": 862.09, "end": 862.95, "word": " وفجأة", "probability": 0.982421875}, {"start": 862.95, "end": 863.77, "word": " بيثبت", "probability": 0.97822265625}, {"start": 863.77, "end": 864.59, "word": " المنحنة", "probability": 0.98984375}, {"start": 864.59, "end": 865.23, "word": " متى", "probability": 0.896240234375}, {"start": 865.23, "end": 865.81, "word": " هيثبت", "probability": 0.9730224609375}, {"start": 865.81, "end": 866.03, "word": " عند", "probability": 0.6455078125}, {"start": 866.03, "end": 866.63, "word": " وصول", "probability": 0.9889322916666666}, {"start": 866.63, "end": 866.87, "word": " إلى", "probability": 0.428955078125}, {"start": 866.87, "end": 867.71, "word": " Saturation", "probability": 0.792236328125}, {"start": 867.71, "end": 868.55, "word": " Point", "probability": 0.318115234375}], "temperature": 1.0}, {"id": 39, "seek": 87534, "start": 870.04, "end": 875.34, "text": "طريقة العمل بالتفصيل هنحكي عنها لما نشتغلها في المعمل", "tokens": [9566, 16572, 28671, 18863, 42213, 20666, 2655, 5172, 9381, 26895, 8032, 1863, 5016, 4117, 1829, 18871, 11296, 5296, 15042, 8717, 8592, 2655, 17082, 1211, 11296, 8978, 9673, 25957, 1211], "avg_logprob": -0.03237304575741291, "compression_ratio": 1.225, "no_speech_prob": 5.960464477539063e-08, "words": [{"start": 870.04, "end": 870.62, "word": "طريقة", "probability": 0.9918619791666666}, {"start": 870.62, "end": 871.06, "word": " العمل", "probability": 0.978759765625}, {"start": 871.06, "end": 871.98, "word": " بالتفصيل", "probability": 0.98388671875}, {"start": 871.98, "end": 872.72, "word": " هنحكي", "probability": 0.94501953125}, {"start": 872.72, "end": 872.96, "word": " عنها", "probability": 0.993896484375}, {"start": 872.96, "end": 873.3, "word": " لما", "probability": 0.91015625}, {"start": 873.3, "end": 874.26, "word": " نشتغلها", "probability": 0.989501953125}, {"start": 874.26, "end": 874.6, "word": " في", "probability": 0.9814453125}, {"start": 874.6, "end": 875.34, "word": " المعمل", "probability": 0.9420572916666666}], "temperature": 1.0}], "language": "ar", "language_probability": 1.0, "duration": 879.8273125, "duration_after_vad": 860.2153437500004}
\ No newline at end of file
diff --git a/PL9fwy3NUQKwZ1Q7zOg1ObgufPMjRI3WY1/qvn-U_MgMmU_raw.srt b/PL9fwy3NUQKwZ1Q7zOg1ObgufPMjRI3WY1/qvn-U_MgMmU_raw.srt
new file mode 100644
index 0000000000000000000000000000000000000000..587e0ca4a208af1e07f357e837e89e9734ccb6bb
--- /dev/null
+++ b/PL9fwy3NUQKwZ1Q7zOg1ObgufPMjRI3WY1/qvn-U_MgMmU_raw.srt
@@ -0,0 +1,884 @@
+1
+00:00:00,000 --> 00:00:03,860
+At the active site هذا كيف بيثبت الـ non
+
+2
+00:00:03,860 --> 00:00:07,100
+-competitive؟ الـ non-competitive بيجي بيرتبط في
+
+3
+00:00:07,100 --> 00:00:11,560
+موقع اسمه الـ steric site موجود في الإنزع تمام؟
+
+4
+00:00:12,150 --> 00:00:16,930
+مجرد ما يرتبط الـ Non-competitive Act بيعمل تغيير
+
+5
+00:00:16,930 --> 00:00:21,110
+وتحوير لشكل الـ Active Site بيغير شكل الـ Active
+
+6
+00:00:21,110 --> 00:00:24,790
+Site فبيبطل الـ Active Site شكله ملائم للـ
+
+7
+00:00:24,790 --> 00:00:27,930
+Substrate بتيجي الـ Substrate ده ترتبط بتلاقي
+
+8
+00:00:27,930 --> 00:00:32,250
+الموقع مختلف الـ Active Site مش ملائم لها، غير
+
+9
+00:00:32,250 --> 00:00:36,850
+ملائم للارتباط فبتقدرش ترتبطفبيثبت ارتباط الـ
+
+10
+00:00:36,850 --> 00:00:41,550
+«substrate» مع الـ «enzyme» في موقع الـ «active
+
+11
+00:00:41,550 --> 00:00:46,410
+site» من غلال تغيير شكل الـ «active site» فبيغير
+
+12
+00:00:46,410 --> 00:00:49,150
+شكل الـ «active site» وبدرش يرتبط فيها الـ
+
+13
+00:00:49,150 --> 00:00:51,650
+«substrate» طب هل أنا لو زودت تركيز الـ
+
+14
+00:00:51,650 --> 00:00:55,050
+«substrate» بدي تنحل المشكلة؟ لأ، بتنحلش لأن
+
+15
+00:00:55,050 --> 00:01:00,190
+المشكلة أساسها ارتباطه في موقع آخر وتغيير شكل الـ
+
+16
+00:01:00,190 --> 00:01:01,690
+«active site»
+
+17
+00:01:05,860 --> 00:01:09,660
+الكلمة «KM» لم تتغير «KM» اللي بتعبر عن تركيز
+
+18
+00:01:09,660 --> 00:01:14,100
+السبسترات في حالة non-competitive inhibitor ما
+
+19
+00:01:14,100 --> 00:01:17,700
+بتتغير، ليش؟ هاي non-competitive inhibitor don't
+
+20
+00:01:17,700 --> 00:01:20,660
+interfere with the binding of substrate to enzyme
+
+21
+00:01:20,660 --> 00:01:26,980
+ما بتأثر على ارتباط السبسترات مع الانزانالأنزين
+
+22
+00:01:26,980 --> 00:01:30,620
+يظهر نفس الـKM في الـprisons أو عدم وجود
+
+23
+00:01:30,620 --> 00:01:34,580
+نانكومبيتيف انهيبتور بيحكي إن في حال وجود
+
+24
+00:01:34,580 --> 00:01:39,580
+نانكومبيتيف انهيبتور أو عدم وجوده، KM نفسها، KM مش
+
+25
+00:01:39,580 --> 00:01:42,380
+هتتغير، لأنه أنا مهما أزود مش زي الأولى
+
+26
+00:01:42,380 --> 00:01:45,400
+الـcompetitive inhibitor، مش هنحل المشكلة، حيث أنا
+
+27
+00:01:45,400 --> 00:01:49,260
+بنزود تركيز السبستريات، فبتغير KM، بتزيد، لكن في
+
+28
+00:01:49,260 --> 00:01:52,300
+هذه الحالة أنا مافيش داعي أزود أصلا تركيز
+
+29
+00:01:52,300 --> 00:01:55,920
+السبستريات لأن أنا مش هتأثر، مش هتحل المشكلةفـ KM
+
+30
+00:01:55,920 --> 00:02:02,140
+نفسها Vmax بتجلب هلجيت أنا لو فيش عندى inhibitor
+
+31
+00:02:02,140 --> 00:02:05,200
+هاصل لـ Vmax لكن لو في عندى non-competitive
+
+32
+00:02:05,200 --> 00:02:08,760
+inhibitor خلاص شوّه شكل الـ active site مش هتقدر
+
+33
+00:02:08,760 --> 00:02:12,960
+ترتبط الـ substrate فانا مش هاصل لـ Vmax فVmax في
+
+34
+00:02:12,960 --> 00:02:16,520
+حالة non-competitive inhibitor هتجلب increasing
+
+35
+00:02:16,520 --> 00:02:19,380
+the concentration of substrate doesn't over count
+
+36
+00:02:19,380 --> 00:02:23,640
+non-competitive inhibitorNon-competitive inhibitor
+
+37
+00:02:23,640 --> 00:02:26,480
+decreases the Vmax of the reaction الـ Non
+
+38
+00:02:26,480 --> 00:02:30,600
+-competitive inhibitor بيجلل الـ Vmax Non
+
+39
+00:02:30,600 --> 00:02:33,680
+-competitive inhibitor therefore simply reduces
+
+40
+00:02:33,680 --> 00:02:37,880
+the amount of active enzyme بيجلل كمية الإنزين
+
+41
+00:02:37,880 --> 00:02:41,560
+النشطة اللي هو موقع الـ Active enzyme فهيجلل الـ
+
+42
+00:02:41,560 --> 00:02:49,490
+Vmax ولكن مش هيؤثر على KMهنا مخطط بيوضح في حال
+
+43
+00:02:49,490 --> 00:02:53,150
+وجود non-inhibitor أو non-competitive inhibitor
+
+44
+00:02:53,150 --> 00:02:56,210
+إذا ما في حالة وجود أو عدم وجود inhibitor في حالة
+
+45
+00:02:56,210 --> 00:02:59,990
+وجود non-competitive inhibitor في حالة عدم وجود
+
+46
+00:02:59,990 --> 00:03:03,710
+inhibitor القاعدة المعروفة اللي حكينا عنها إنه كل
+
+47
+00:03:03,710 --> 00:03:07,330
+ما زاد تركيز الـsubstrate بتزيد سرعة التفاعل
+
+48
+00:03:07,330 --> 00:03:12,330
+الكيميائي ونشاط الإنزيموصولًا إلى نقطة التشبع
+
+49
+00:03:12,330 --> 00:03:16,250
+للـSaturation Point اللي هي عندها Vmax يثبت
+
+50
+00:03:16,250 --> 00:03:19,970
+المنحنة وصولًا إلى Vmax طيب، في حال وجود Non
+
+51
+00:03:19,970 --> 00:03:24,350
+-competitive inhibitor لكن هذا بيأثر على الـActive
+
+52
+00:03:24,350 --> 00:03:28,110
+site فما بتقدر ترتبط الـSubstrate في الـActive
+
+53
+00:03:28,110 --> 00:03:35,030
+siteفهيجيل الـ Vmax هل هتتأثر KM هتتأثر تركيز الـ
+
+54
+00:03:35,030 --> 00:03:39,630
+Substrate لأ نفسها وانما مين اللي هتقول الـ Vmax
+
+55
+00:03:39,630 --> 00:03:43,330
+اللي هي سرعة التفاعل اقصى سرعة الا انا مش هقدر
+
+56
+00:03:43,330 --> 00:03:46,130
+اصلا اللي هتجيل Vmax
+
+57
+00:03:47,790 --> 00:03:50,750
+في النهاية الملخص «competitive inhibitor» عندنا
+
+58
+00:03:50,750 --> 00:03:53,070
+أنواع الـ «inhibitor» «competitive» و «non
+
+59
+00:03:53,070 --> 00:03:55,610
+-competitive» في حالة الـ «competitive»، إيش
+
+60
+00:03:55,610 --> 00:04:00,010
+هيتغير؟ «KM» بتزيد، تركيز الـ «substrate» بزيد أما
+
+61
+00:04:00,010 --> 00:04:04,070
+«Vmax» ما بتتغير، أقصى سرعة التفاعل هي نفسها «non
+
+62
+00:04:04,070 --> 00:04:08,430
+-competitive inhibitor» «KM» ما بتتغير، no change،
+
+63
+00:04:08,430 --> 00:04:15,040
+بط «Vmax» بتجيلب، لكن أقصى سرعة التفاعل بيتجيلببعض
+
+64
+00:04:15,040 --> 00:04:18,500
+المصطلحات الأغلبية حكينا عنها أولاً الـ Active
+
+65
+00:04:18,500 --> 00:04:22,580
+site الـ Active site حكينا هو موقع في الإنزيم
+
+66
+00:04:22,580 --> 00:04:26,920
+بيرتبط فيها الـ substrate لحدوث التفاعل region of
+
+67
+00:04:26,920 --> 00:04:30,340
+enzyme molecule find the substrate and carry the
+
+68
+00:04:30,340 --> 00:04:33,920
+catalytic reaction موقع في الإنزيم بيرتبط فيها الـ
+
+69
+00:04:33,920 --> 00:04:37,540
+substrate لحدوث التفاعل فبالإنزيم، حكي أنا تعرفي
+
+70
+00:04:37,540 --> 00:04:41,540
+الإنزيم هو محفز بيولوجي غالباً بيكون globular
+
+71
+00:04:41,540 --> 00:04:45,760
+protein عبارة عن البروتينبينتج من أجسام الكائنات
+
+72
+00:04:45,760 --> 00:04:50,640
+الحية لتسريع التفاعل الكيميائي وبيطلع بعد التفاعل
+
+73
+00:04:50,640 --> 00:04:56,520
+كما هو، بدون حدوث أي تدمير أو تغيير على شكله طيب،
+
+74
+00:04:56,520 --> 00:05:00,100
+«KM»، «KM» برضه حكي هنا إنه هي عبارة عن تركيز
+
+75
+00:05:00,100 --> 00:05:03,920
+الـsubstrate عند نص Vmax عند الـhalf maximum
+
+76
+00:05:03,920 --> 00:05:07,540
+velocity substrate concentration at half maximum
+
+77
+00:05:07,540 --> 00:05:12,620
+velocity«Vmax» حكينا إنه هي أقصى سرعة للتفاعل
+
+78
+00:05:12,620 --> 00:05:17,240
+«Maximum initial velocity of enzyme reaction» أقصى
+
+79
+00:05:17,240 --> 00:05:21,680
+سرعة لمعدل حدود التفاعل الكيميائي ولعمل الإنزين
+
+80
+00:05:21,680 --> 00:05:26,720
+عندها بموصل إلى نقطة الـ «saturation point» إنه
+
+81
+00:05:26,720 --> 00:05:30,680
+بيصير التفاعل constant تابت بيصير خلاص بيثبت
+
+82
+00:05:30,680 --> 00:05:34,740
+التفاعل لأنه عندنا «saturating substrate level»
+
+83
+00:05:34,740 --> 00:05:39,530
+عنده نقطة تشبع من السبستراتالـ catalyst اللي هو
+
+84
+00:05:39,530 --> 00:05:45,570
+المحفز وأي مادة بتسرق حدوط التفاعل البطيء anything
+
+85
+00:05:45,570 --> 00:05:47,930
+that speeds up a chemical reaction that is
+
+86
+00:05:47,930 --> 00:05:52,890
+occurring slowly أي مادة بتسرق حدوط التفاعل اللي
+
+87
+00:05:52,890 --> 00:05:57,990
+كان يمشي ببطء بسميها catalyst محفز اليوم تطبيقنا
+
+88
+00:05:57,990 --> 00:06:01,030
+العمل هيكون على أنزيم اسمه alkaline phosphate
+
+89
+00:06:04,010 --> 00:06:07,170
+الـ alkaline phosphatase هذا بيعمل على تكسير فصفات
+
+90
+00:06:07,170 --> 00:06:10,030
+جروب بيشتغل على المادة اللي بتحتوي على مجموعة
+
+91
+00:06:10,030 --> 00:06:13,710
+فصفات بيكسر هذه الفصفات إذا member of
+
+92
+00:06:13,710 --> 00:06:17,390
+phosphomonoesterase group هو أحد أعضاء مجموعة
+
+93
+00:06:17,390 --> 00:06:22,180
+إنزيمات يطلق عليها phosphomonoesterase groupThey
+
+94
+00:06:22,180 --> 00:06:25,880
+are highly specific and act on only one substrate
+
+95
+00:06:25,880 --> 00:06:31,300
+ومتخصص هيشتغل بس على substrates معين حكينا كل
+
+96
+00:06:31,300 --> 00:06:34,620
+أنزين متخصص بsubstrate معين وهذا لقناع
+
+97
+00:06:34,620 --> 00:06:38,680
+الانفسفاتياز متخصص بsubstrate معينهذه الـ
+
+98
+00:06:38,680 --> 00:06:42,100
+Substrate اللي بتحتوي على فوسفات جروب «الكالاين
+
+99
+00:06:42,100 --> 00:06:46,180
+فوسفاتيز» لديه فوسفات كبير بشكل خاص بـ Ccf3 عنده
+
+100
+00:06:46,180 --> 00:06:49,960
+Range واسع من خصوصيته على الـ Substrate كيف يعني؟
+
+101
+00:06:49,960 --> 00:06:53,560
+هو متخصص بـ Substrate واحد لكن هذا الـ Substrate
+
+102
+00:06:53,560 --> 00:06:56,960
+اللي هو متخصص فيه يحتوي على فوسفات جروب ففي أكتر
+
+103
+00:06:56,960 --> 00:07:00,560
+من مادة في الجسم في أكتر من مكان بتحتوي على فوسفات
+
+104
+00:07:00,560 --> 00:07:05,690
+جروب عشان هيك هو له Broad Range واسعIt is named
+
+105
+00:07:05,690 --> 00:07:08,390
+alkaline phosphatase ليش بيسموها alkaline
+
+106
+00:07:08,390 --> 00:07:14,070
+phosphatase؟ لأنه بيشتغل عن BH9، BH alkaline في
+
+107
+00:07:14,070 --> 00:07:18,490
+عنده مجموعة أخرى بتشتغل عن BH7 بيسموها acid
+
+108
+00:07:18,490 --> 00:07:24,060
+phosphatase، بتشتغل عن BH7بيتعمل دراسة للـ
+
+109
+00:07:24,060 --> 00:07:27,620
+«Alkaline Phosphatase in Vitro» ندرست أو نحدد الـ
+
+110
+00:07:27,620 --> 00:07:30,380
+«Alkaline Phosphatase» في المعنى «Alkaline
+
+111
+00:07:30,380 --> 00:07:33,680
+Phosphatase activity by following the rate of
+
+112
+00:07:33,680 --> 00:07:36,300
+formation of a product» كيف بتتبر «Alkaline
+
+113
+00:07:36,300 --> 00:07:39,740
+Phosphatase»؟ من خلال متابعة تكون الـ product،
+
+114
+00:07:39,740 --> 00:07:43,040
+النواتج، إني بدي أجيب اللينزين هاد أحطله الـ
+
+115
+00:07:43,040 --> 00:07:46,700
+substrate المتخصص فيه يرتبطوا مع بعض ويحولوا إلى
+
+116
+00:07:46,700 --> 00:07:50,960
+product أشوف هذا ال product الناتج من خلالها بدرست
+
+117
+00:07:50,960 --> 00:07:54,450
+اللي هو هذا اللينزينالـ Alkaline Phosphatase اللي
+
+118
+00:07:54,450 --> 00:07:57,730
+بيشتغل على حكاية هنا الـ substrate تحتوي على فسفات
+
+119
+00:07:57,730 --> 00:08:02,570
+grow على alkaline media على pH alkaline تكون بينجل
+
+120
+00:08:02,570 --> 00:08:05,910
+مجموعة الفسفات ي grow فأنا بدي أحطله اليوم
+
+121
+00:08:05,910 --> 00:08:10,770
+substrate هو أربع نيتروفينال فسفات تمام؟ و أحطله
+
+122
+00:08:10,770 --> 00:08:15,310
+alkaline pH وبيشتغل عليها ويحولني إياها الأربع
+
+123
+00:08:15,310 --> 00:08:19,720
+نيتروفينالهذه الأربع نيتروفينول بقراها على جهاز
+
+124
+00:08:19,720 --> 00:08:25,300
+Spectrophotometer على طول موجي 400 نانومتر بقراها
+
+125
+00:08:25,300 --> 00:08:29,040
+بجيس كمية الضوء اللي امتصتها العين لأن لونها بتكون
+
+126
+00:08:29,040 --> 00:08:33,660
+bright yellow ولكن باقي المكونات والنواياتش بتكون
+
+127
+00:08:33,660 --> 00:08:37,540
+لونها بتكون colorless فانا بدي أقرا بس للأربع
+
+128
+00:08:37,540 --> 00:08:42,160
+نيتروفينول فبشوف من خلال هذه القراعات بعرف نشاط
+
+129
+00:08:42,160 --> 00:08:46,610
+الانزينإذا أنا عندي إنزيم ألكالينفوسفاتيز بحطله
+
+130
+00:08:46,610 --> 00:08:50,470
+substrate وتتكون عندي product زي المعادل اللي
+
+131
+00:08:50,470 --> 00:08:53,790
+حكيناها أولاً بإنزايم زائد substrate بيعطيني
+
+132
+00:08:53,790 --> 00:08:57,050
+إنزايم substrate complex وبعدين بيبتدي عندي إنزايم
+
+133
+00:08:57,050 --> 00:09:00,210
+و product مين أنا عند الإنزايم والsubstrate؟
+
+134
+00:09:00,210 --> 00:09:03,670
+الإنزايم هو ألكالينفوسفاتيز زائد substrate أربعة
+
+135
+00:09:03,670 --> 00:09:07,530
+نيتروفين والفوسفات إيش الناتج بدي يطلع عندي؟ بدي
+
+136
+00:09:07,530 --> 00:09:10,270
+يطلع عندي إنزيم و product الإنزيم هو
+
+137
+00:09:10,270 --> 00:09:14,010
+للكلينفوسفاتيز، هيطلع زي ما هوزائد مجموعة الـ
+
+138
+00:09:14,010 --> 00:09:18,430
+Phosphate Group بيكسّرها هذا الإنزيم، زاد 4
+
+139
+00:09:18,430 --> 00:09:22,190
+نيتروفينول. الأربع نيتروفينول هذه اللي هي yellow
+
+140
+00:09:22,190 --> 00:09:25,630
+color، بكلها أصفر، بقرأ الـ absorption، read
+
+141
+00:09:25,630 --> 00:09:30,310
+absorption، لها 400 نانومتر، بقرألها الانتصاص على
+
+142
+00:09:30,310 --> 00:09:36,560
+400 نانومتر، طول موجي 400.كيف بدأ أجيز نسمة الـ
+
+143
+00:09:36,560 --> 00:09:39,360
+«measurement of alkaline phosphatase activity»
+
+144
+00:09:39,360 --> 00:09:42,080
+بناء على مادة الـ«substrate» اللي هي هذه
+
+145
+00:09:42,080 --> 00:09:45,260
+الـ«artificial» المصنعة هي الـ«نيتروفينالفوسفات»
+
+146
+00:09:45,260 --> 00:09:47,560
+هذا الـ«substrate» بيرتبط مع الـ«alkaline
+
+147
+00:09:47,560 --> 00:09:50,520
+phosphatase» وبيعطينا product حكينا الـ«product»
+
+148
+00:09:50,520 --> 00:09:55,430
+ينتج لون يلوبيحكيلي إن شدة اللون، intensity of
+
+149
+00:09:55,430 --> 00:09:58,930
+yellow color شدة اللون هذا الأصفر بتعبّرلي عن
+
+150
+00:09:58,930 --> 00:10:02,130
+degree to which enzyme has acted upon a substrate
+
+151
+00:10:02,130 --> 00:10:07,430
+عن درجة ارتباط الانزين مع ال substrate، درجة عمل
+
+152
+00:10:07,430 --> 00:10:12,590
+الانزين شدة اللون تعبّر على درجة عمل الانزين
+
+153
+00:10:14,750 --> 00:10:17,630
+في عندنا هنا تعريف الـ enzyme kinetic وهذا حكينا
+
+154
+00:10:17,630 --> 00:10:20,490
+عنه في الأول أنه ي study في rate at which enzyme
+
+155
+00:10:20,490 --> 00:10:24,690
+works دراسة معدل حدوث التفاعل طب أنا كيف بدي أدرس
+
+156
+00:10:24,690 --> 00:10:27,770
+هنا معدل حدوث التفاعل؟ اليوم الدراسة بتكون على
+
+157
+00:10:27,770 --> 00:10:31,630
+أنزيم الألقالي الفوسفاتينبدي أدرس معدل حدوث
+
+158
+00:10:31,630 --> 00:10:35,570
+الـAlkaline Phosphatase بناءً على أيش؟ اليوم بدي
+
+159
+00:10:35,570 --> 00:10:38,850
+أدرس بناءً على تغيير في تركيز الـsubstrate يعني
+
+160
+00:10:38,850 --> 00:10:43,830
+أنا في نهاية المعمل بعد شغلي بدي أشوف العلاقة ما
+
+161
+00:10:43,830 --> 00:10:47,860
+بين تركيز الـsubstrate وسرعة التفاعل الكيميائيأحنا
+
+162
+00:10:47,860 --> 00:10:52,040
+حكينا إن هذه العلاقة خطية وصولا إلى Vmax إلى نقطة
+
+163
+00:10:52,040 --> 00:10:55,320
+التشبوع اللي هي الـ saturation point بتبطل العلاقة
+
+164
+00:10:55,320 --> 00:10:58,460
+خطية وإنما بيسبق فأنا اليوم بدأ أدرس هذه العلاقة
+
+165
+00:10:58,460 --> 00:11:03,880
+كيف بدأ أدرسها؟محكيلة إن نجيب أدت يوبات ونحط فيها
+
+166
+00:11:03,880 --> 00:11:07,340
+تركيز مختلفة من الـ substrates يعني مثلا الأولى
+
+167
+00:11:07,340 --> 00:11:12,560
+بدي أحط فيها تركيز substrates خمسة التانى تركيز
+
+168
+00:11:12,560 --> 00:11:15,160
+الـ substrates يكون فيها عشرة واللي بعدها تركيز
+
+169
+00:11:15,160 --> 00:11:19,160
+الـ substrates مثلا خمستاشر بعدها عشرين بعدها خمسة
+
+170
+00:11:19,160 --> 00:11:23,140
+وعشرين بعدها بدي أحط تلاتين خمسة وتلاتين وها كده
+
+171
+00:11:23,140 --> 00:11:26,320
+كل تيوب ده تكون تركيز الـ substrates فيها أعلى من
+
+172
+00:11:26,320 --> 00:11:29,770
+الـ tube اللي جابلهصار عندى تيوبات فيها Substrate
+
+173
+00:11:29,770 --> 00:11:34,250
+بتركيز معين بدي هجيبى الـ Enzyme عشان يرتبط مع الـ
+
+174
+00:11:34,250 --> 00:11:37,330
+Substrate ويكوّن لـ Product هلجيت بحط على الـ Tube
+
+175
+00:11:37,330 --> 00:11:39,950
+الأول كلهم نفس الكمية من الـ Enzyme بدي أحط عليها
+
+176
+00:11:39,950 --> 00:11:43,230
+Enzyme ييجى الـ Enzyme بيرتبط مع الـ Substrate في
+
+177
+00:11:43,230 --> 00:11:46,510
+الـ Tube الأول وبيعطيني Product بدي أقرأه في جهاز
+
+178
+00:11:46,510 --> 00:11:49,570
+الـ Spectrophotometer أقرأله الـ Absorbent بعدين
+
+179
+00:11:49,570 --> 00:11:52,690
+باخد الـ Tube التانى و أقرأ الـ Absorbentأخد الـ
+
+180
+00:11:52,690 --> 00:11:54,930
+tube التالتة أحط عليها أنزين و أقرأ الـ absorption
+
+181
+00:11:54,930 --> 00:11:58,750
+و الـ tube الرابع و هكذا لكل tube بيصير كل tube
+
+182
+00:11:58,750 --> 00:12:02,230
+فيها أنزين و substrate حطيت بالأول بعدين أنزين و
+
+183
+00:12:02,230 --> 00:12:05,510
+بعدين ده اقرأ بيتكون عندي بسيبها شوية حتى يتفاعل
+
+184
+00:12:05,510 --> 00:12:08,650
+الانزين مع ال substrate و يعطيني product اتكون ال
+
+185
+00:12:08,650 --> 00:12:11,270
+product باخدها بقراها في جهاز الـ
+
+186
+00:12:11,270 --> 00:12:15,510
+spectrophotometerالجهاز الـ Spectrophotometer
+
+187
+00:12:15,510 --> 00:12:20,250
+هيعطيني Absorbance قراية الانتصار وانا عندي معروف
+
+188
+00:12:20,250 --> 00:12:23,670
+عرفت تركيز الـ Substrate لكل تيوب مختلف عن التانية
+
+189
+00:12:23,670 --> 00:12:26,730
+حكينا على فرض الأولى خمسة، التانية عشرة، التلبعيدة
+
+190
+00:12:26,730 --> 00:12:29,930
+خمستاشر، عشرين، وخمسة وعشرين تركيز الـ Substrate
+
+191
+00:12:29,930 --> 00:12:35,110
+هلجت الـ Absorbance لكل تيوب هيختلف عن التانيةوهذا
+
+192
+00:12:35,110 --> 00:12:38,350
+الـ Absorbance حكينا بيختلف حسب شدة اللون، حسب عمل
+
+193
+00:12:38,350 --> 00:12:42,450
+الانزين فجيتي؟ مثلا الأولى قرأت الـ Absorbance
+
+194
+00:12:42,450 --> 00:12:45,590
+إليها point واحد، التانية point اتنين، اللي بعدها
+
+195
+00:12:45,590 --> 00:12:49,470
+point تلاتة، اللي بعدها point أربع كل مزود تركيز
+
+196
+00:12:49,470 --> 00:12:53,770
+الـ substrate بزيد ارتباط الانزين مع الـ substrate
+
+197
+00:12:53,770 --> 00:12:58,270
+فهيزيد العمل، هيزيد التفاعل، هتزيد الـ Absorbance،
+
+198
+00:12:58,270 --> 00:13:02,190
+تمام؟وصولا إلى وين هذا؟ هي هي تظل يزيد لما لا
+
+199
+00:13:02,190 --> 00:13:06,670
+نهاية؟ لأ، حكينا أول المعمل إنه بيظل يزيد لوصوله
+
+200
+00:13:06,670 --> 00:13:10,070
+إلى نقطة الـ Saturation Point يعني أنا في النهاية
+
+201
+00:13:10,070 --> 00:13:13,750
+بعد ما أشتغل شغلي وبعد ما أحط التيوبات، أحط فيها
+
+202
+00:13:13,750 --> 00:13:17,290
+تركيز مختلف من الـ Substrate أضيف عليها Enzyme،
+
+203
+00:13:17,290 --> 00:13:20,410
+كمية Enzyme، يتفاعلوا مع بعض ويعطيني Product
+
+204
+00:13:20,410 --> 00:13:24,130
+أقراها على جهاز الـ Spectrum، تطلع عندي Absorbance
+
+205
+00:13:24,130 --> 00:13:26,830
+صار عندي معروف Concentration للـ Substrate و
+
+206
+00:13:26,830 --> 00:13:31,770
+Absorbanceبدي أرسم مخطط، Standard K هل أنت كيف
+
+207
+00:13:31,770 --> 00:13:35,210
+متوقع أنه يكون شكل المدخول مخطط؟ بناء على العلاقة
+
+208
+00:13:35,210 --> 00:13:37,890
+اللي حكينا عنها اللي هي العلاقة بين الـSubstrate
+
+209
+00:13:37,890 --> 00:13:43,310
+Concentration وسرعة التفاعل أو معدل حدوث التفاعل
+
+210
+00:13:43,310 --> 00:13:47,390
+العلاقة الحكينا عنها الطرضية وصولا إلى Vmax، إلى
+
+211
+00:13:47,390 --> 00:13:51,410
+الـMaximum Velocity فبناء عليها، هشوف النتاج بتظل
+
+212
+00:13:51,410 --> 00:13:56,130
+هتزيد معي وفجأة بتثبت النتاج، هوصل إلى نقطة
+
+213
+00:13:56,130 --> 00:14:01,090
+Saturation Pointالى ثباتالنتاج احنا في النهاية
+
+214
+00:14:01,090 --> 00:14:06,350
+بنرسم هذا المخطط بدي أرسم على الخط الصيني اللي هي
+
+215
+00:14:06,350 --> 00:14:09,890
+نقطة الخط الصيني بدي أرسم عليها الـ Concentration
+
+216
+00:14:09,890 --> 00:14:13,030
+للـ Substrate الخط الصادى بدي أرسم الـ Absorbance
+
+217
+00:14:13,030 --> 00:14:15,950
+كل نقطة جديش إليها Concentration وجديش إليها
+
+218
+00:14:15,950 --> 00:14:20,170
+Substrate وأرسمها وأرسم اللي يطلع معايا المنحنة
+
+219
+00:14:20,170 --> 00:14:25,230
+هيطلع معايا علاقة ترضية وفجأة بيثبت المنحنة متى
+
+220
+00:14:25,230 --> 00:14:31,060
+هيثبت عند وصول إلى Saturation Pointطريقة العمل
+
+221
+00:14:31,060 --> 00:14:35,340
+بالتفصيل هنحكي عنها لما نشتغلها في المعمل
+
diff --git a/PL9fwy3NUQKwZ1Q7zOg1ObgufPMjRI3WY1/tYZzyB0o5Ps.srt b/PL9fwy3NUQKwZ1Q7zOg1ObgufPMjRI3WY1/tYZzyB0o5Ps.srt
new file mode 100644
index 0000000000000000000000000000000000000000..c8585baedf97c28cb6f545ab4f67076fcb055b1d
--- /dev/null
+++ b/PL9fwy3NUQKwZ1Q7zOg1ObgufPMjRI3WY1/tYZzyB0o5Ps.srt
@@ -0,0 +1,2257 @@
+1
+00:00:04,790 --> 00:00:08,130
+اليوم بدنا نحكي بموضوع الـ quantitative test for 
+
+2
+00:00:08,130 --> 00:00:14,030
+protein هو فحوصات للكشف عن البروتين ولكن هذه 
+
+3
+00:00:14,030 --> 00:00:19,030
+الفحوصات نوعها quantitative، quantitative معناها كم 
+
+4
+00:00:19,030 --> 00:00:23,730
+يعني الفحوصات كمية، يعني بدي يطلع معانا في الآخر 
+
+5
+00:00:23,730 --> 00:00:27,530
+تركيز للبروتين، مش أنه ظهور لون positive أو 
+
+6
+00:00:27,530 --> 00:00:32,010
+negative زي ال qualitative، إنما quantitative يعني 
+
+7
+00:00:32,010 --> 00:00:36,010
+بدي يظهر عندي تركيز للبروتين، بدي يطلع عندي رقم 
+
+8
+00:00:36,010 --> 00:00:43,650
+للبروتين، جداش نسبة البروتين، بدنا نحكي عن إيش اسمه 
+
+9
+00:00:43,650 --> 00:00:48,630
+colorimetric assay، colorimetric assay معناها تحليل 
+
+10
+00:00:48,630 --> 00:00:54,260
+اللون، فحوصات تحليل اللون، لا كيف هذه الفحوصات هي 
+
+11
+00:00:54,260 --> 00:00:59,340
+مجموعة من الفحوصات من خلالها بعرف تركيز أي مادة 
+
+12
+00:00:59,340 --> 00:01:04,820
+بناء على التغير في اللون، مجموعة من الفحوصات من 
+
+13
+00:01:04,820 --> 00:01:10,520
+خلالها بعرف تركيز أي مادة بناء على التغير في اللون 
+
+14
+00:01:10,520 --> 00:01:14,180
+the law for indirect determination of specific 
+
+15
+00:01:14,180 --> 00:01:20,000
+concentration تسمح بمعرفة تركيز المواد، زي إيش هي 
+
+16
+00:01:20,000 --> 00:01:24,500
+المواد الممكن تعرف تركيزها؟ بروتين، كربوهيدرات، أو 
+
+17
+00:01:24,500 --> 00:01:28,460
+انزين بناء على ياش color exchange، بناء على التغير 
+
+18
+00:01:28,460 --> 00:01:32,420
+باللون، this reaction can be performed directly 
+
+19
+00:01:32,420 --> 00:01:35,900
+inside the spectrophotometer، طب وين هي لجيت أنا 
+
+20
+00:01:35,900 --> 00:01:39,360
+بحط المواد و بتغير لونها؟ طب مين بدي يجيس كمية 
+
+21
+00:01:39,360 --> 00:01:43,080
+التغير في هذا اللون و يطلعلي التركيز؟ بناء على 
+
+22
+00:01:43,080 --> 00:01:48,420
+جهاز اسمه جهاز spectrophotometer، جهاز 
+
+23
+00:01:48,420 --> 00:01:56,330
+spectrophotometer، وهو جهاز قياس الطيف الضوء، جهاز 
+
+24
+00:01:56,330 --> 00:02:03,710
+spectrophotometer هو جهاز لقياس كمية الضوء التي 
+
+25
+00:02:03,710 --> 00:02:10,290
+تنتصها العينة بناء على قانون Beer-Lambert Law، هو 
+
+26
+00:02:10,290 --> 00:02:16,070
+جهاز لقياس كمية الضوء التي تنتصها العينة بناء على 
+
+27
+00:02:16,070 --> 00:02:21,520
+قانون Beer-Lambert Law، وقانون هذا إيش بيحكي؟ بيحكي 
+
+28
+00:02:21,520 --> 00:02:26,440
+the intensity of color and absorption is directly 
+
+29
+00:02:26,440 --> 00:02:30,720
+proportional to the concentration، إنه كتافة لون 
+
+30
+00:02:30,720 --> 00:02:34,900
+المادة، اللون اللي أنا بحطه، بحب بعد ما أخلط المادة 
+
+31
+00:02:34,900 --> 00:02:38,800
+المراد قياسها على ال reaction، اللون اللي بيظهر عندي 
+
+32
+00:02:38,800 --> 00:02:43,700
+كتافة اللون هاي بتتناسب تناسب طردي مع تركيز المادة 
+
+33
+00:02:43,700 --> 00:02:48,110
+يعني كل ما زادت شدة اللون، بيكون تركيز المادة 
+
+34
+00:02:48,110 --> 00:02:56,790
+الموجود أعلى، كل ما قلت شدة اللون، هيقل 
+
+35
+00:02:56,790 --> 00:03:02,290
+تركيز المادة، علاقة طردية بين كتافة لون المادة و 
+
+36
+00:03:02,290 --> 00:03:04,790
+تركيز هذه المادة 
+
+37
+00:03:07,450 --> 00:03:11,870
+إيش هي الطرق لتحديد تركيز البروتين؟ method for 
+
+38
+00:03:11,870 --> 00:03:16,010
+protein determination، طرق تحديد تركيز البروتين، كيف 
+
+39
+00:03:16,010 --> 00:03:19,750
+أنا بدي عندي عينة و بدي أعرف تركيز البروتين فيها؟ 
+
+40
+00:03:19,750 --> 00:03:22,890
+في عدة طرق، في أحد الطرق هي الـ 
+
+41
+00:03:22,890 --> 00:03:27,630
+spectrophotometry based on UV on ultraviolet light 
+
+42
+00:03:27,630 --> 00:03:31,110
+اللي هو طريقة الـ spectrophotometry، جهاز الـ 
+
+43
+00:03:31,110 --> 00:03:35,830
+spectrophotometer، بناء على مشاعر فوضوى نفسجية 
+
+44
+00:03:35,830 --> 00:03:43,090
+ultraviolet-law هي طريقة تقليدية وقديمة، البروتين 
+
+45
+00:03:43,090 --> 00:03:48,190
+يُكْسِرُ ultraviolet-light في 280 نانومتر، بتم قياسه 
+
+46
+00:03:48,190 --> 00:03:54,530
+الضوء الممتص عند ضوء طول موجي 280 نانو متر، لكن 
+
+47
+00:03:54,530 --> 00:03:58,010
+اكتشفوا أن الـ ultraviolet light هذي بتأثر على صحة 
+
+48
+00:03:58,010 --> 00:04:03,230
+الإنسان وأنها شعة ضارة، فلجأوا إلى وجود طريقة أخرى 
+
+49
+00:04:03,230 --> 00:04:06,710
+اللي هي الطريقة الثانية، الـ dye binding assay أو 
+
+50
+00:04:06,710 --> 00:04:12,570
+اسمها Bradford assay، dye binding assay أو Bradford 
+
+51
+00:04:12,570 --> 00:04:17,820
+assay، تعتمد على وجود صبغة ترتبط مع البروتين، زي ما 
+
+52
+00:04:17,820 --> 00:04:21,420
+وجود البروتين في العينة، تيجي هذه الصبغة ترتبط مع 
+
+53
+00:04:21,420 --> 00:04:26,520
+البروتين و بتعطيني لون و بجيس هذا اللون، depend on 
+
+54
+00:04:26,520 --> 00:04:30,500
+dye which bind to protein and give color، هذه طريقة 
+
+55
+00:04:30,500 --> 00:04:34,880
+اسمها dye binding assay أو Bradford assay، وبعدين 
+
+56
+00:04:34,880 --> 00:04:39,840
+طوروها لطريقة أخرى هي ال copper based assay، الـ 
+
+57
+00:04:39,840 --> 00:04:43,800
+copper-based assay هي فحوصات تعتمد على وجود الـ 
+
+58
+00:04:43,800 --> 00:04:48,940
+copper، بيجيسه كمية البروتين بناء على وجود الـ copper 
+
+59
+00:04:48,940 --> 00:04:52,920
+وهي تلت طرق تعتمد على وجود الـ copper اللي هي أول 
+
+60
+00:04:52,920 --> 00:04:58,280
+طريقة الـ Lowry-Folin method، بيكنينك كونك أسد أساي 
+
+61
+00:04:58,280 --> 00:05:03,100
+Lowry-Folin method، بيكنينك كونك أسد أساي، فيه هنا 
+
+62
+00:05:03,100 --> 00:05:08,960
+الـ biuret method، وهذول الطرق اللي بتعتمد على وجود 
+
+63
+00:05:08,960 --> 00:05:14,160
+الـ copper هي مستخدمة هي الـ routine use، ال bio-red 
+
+64
+00:05:14,160 --> 00:05:17,720
+هي الأكثر واحدة مستخدمة وهي اللي بيستخدموها هي 
+
+65
+00:05:17,720 --> 00:05:22,360
+simple، بسيطة وسهلة، جميلة وسريعة، بتعتمد على أنه 
+
+66
+00:05:22,360 --> 00:05:26,760
+يتكون لون، هذا اللون بيعتمد على قانون Beer's law 
+
+67
+00:05:26,760 --> 00:05:31,860
+أنه تتناسب تناسب طردي شدة اللون مع تركيز المادة، كل 
+
+68
+00:05:31,860 --> 00:05:36,960
+ما زاد شدة اللون، بزيد تركيز المادة، كل هذه الطرق 
+
+69
+00:05:36,960 --> 00:05:39,820
+اللي هي الـ copper based assay بتحتمد، all this 
+
+70
+00:05:39,820 --> 00:05:43,320
+method depends on the presence of cupric ion، يعني 
+
+71
+00:05:43,320 --> 00:05:46,800
+سواء الـ Lowry-Folin method، الـ bicinchoninic acid 
+
+72
+00:05:46,800 --> 00:05:51,380
+والـ biuret method، هذول التلات طرق بتحتمد على وجود 
+
+73
+00:05:51,380 --> 00:05:56,280
+الـ cupric ion، أن هو ريجنت إيري أيجنت من مكوناته ال 
+
+74
+00:05:56,280 --> 00:06:01,130
+cupric ion، حكينا قبل هيك إنه الـ cupric الـ cupric 
+
+75
+00:06:01,130 --> 00:06:07,410
+بيعمل chelating agent، عامل متمخلب، بيقدر يعمل عدة 
+
+76
+00:06:07,410 --> 00:06:11,950
+روابط، فبيجي على الروابط الببتيدية الموجودة في 
+
+77
+00:06:11,950 --> 00:06:17,590
+البروتين وبيربط فيها من جميع الجهات، تمام؟ فهذا الـ 
+
+78
+00:06:17,590 --> 00:06:21,990
+cupric ion بيكون موجود في الـ reagent بيجي بيعمل 
+
+79
+00:06:21,990 --> 00:06:25,050
+chelating agent، بيرتبط مع الـ peptide bond 
+
+80
+00:06:25,050 --> 00:06:29,980
+وبيعطيني، لهبحكي ليه إنه الـ cupric ion اللي بيكون 
+
+81
+00:06:29,980 --> 00:06:33,140
+موجود في الـ reaction بيكون على شكل cupric ion 
+
+82
+00:06:33,140 --> 00:06:39,460
+اللي هي Cu+2، بمجرد ما يرتبط في البروتين، إذا 
+
+83
+00:06:39,460 --> 00:06:43,440
+موجود البروتين في العينة أجل cupric ion هذا ارتبط 
+
+84
+00:06:43,440 --> 00:06:46,840
+مع البروتين وربط الروابط اللي بيبتديها مع بعضها 
+
+85
+00:06:46,840 --> 00:06:52,320
+البعض بيصير له reduction إلى cupric ion، الـ cupric 
+
+86
+00:06:52,320 --> 00:06:55,420
+ion اللي موجود في الـ reagent بيكون موجود على شكل 
+
+87
+00:06:55,420 --> 00:07:00,020
+cupric ion، Cu+2، مجرد ما يرتبط مع البروتين 
+
+88
+00:07:00,020 --> 00:07:03,960
+بيصير له reduction، اختزال، إلى cupric ion، Cu+ 
+
+89
+00:07:03,960 --> 00:07:09,760
+ففي عندنا تلت طرق لتحديد تركيز البروتين، أول طريقة 
+
+90
+00:07:09,760 --> 00:07:13,580
+الـ spectrophotometry، وتاني طريقة اللي هي الـ dye 
+
+91
+00:07:13,580 --> 00:07:17,960
+binding assay أو Bradford Assay، والتالتة الـ copper 
+
+92
+00:07:17,960 --> 00:07:19,540
+based assay 
+
+93
+00:07:23,090 --> 00:07:26,190
+هيش الـ aim and significance اللي هي الهدف 
+
+94
+00:07:26,190 --> 00:07:31,930
+والأهمية لما عملنا اليوم، أول شي الأهداف، الـ aim to 
+
+95
+00:07:31,930 --> 00:07:36,550
+perform biuret test for البروتين، لقدرة على إجراء فحص 
+
+96
+00:07:36,550 --> 00:07:40,790
+الـ biuret to estimate the amount of total proteins in 
+
+97
+00:07:40,790 --> 00:07:45,470
+sample، قدرة على تقدير كمية البروتين الموجود في 
+
+98
+00:07:45,470 --> 00:07:50,990
+العينة، وآخر شي to make standard curve، لقدرة على رسم 
+
+99
+00:07:50,990 --> 00:07:55,360
+المنحنى المعياري، الـ standard curve اللي هنحكي عنه 
+
+100
+00:07:55,360 --> 00:07:59,860
+لقدام، طيب الـ significance اللي همية، أنا ليش بدي 
+
+101
+00:07:59,860 --> 00:08:03,800
+أقدر تركيز البروتين، أهمية هذا المعمل أني أعرف 
+
+102
+00:08:03,800 --> 00:08:08,080
+quantification of البروتين، لأن هذا is important and 
+
+103
+00:08:08,080 --> 00:08:10,960
+has many applications in clinical laboratory 
+
+104
+00:08:10,960 --> 00:08:15,640
+practice، لتقدير الأهمية من معملنا أني أقدر تركيز 
+
+105
+00:08:15,640 --> 00:08:19,920
+البروتين، لأن كمية هذا البروتين، أني أعرفها إلها 
+
+106
+00:08:19,920 --> 00:08:25,060
+أهميات وإلها ضرورة، تساعدني في تقييم حالة المريض، 
+
+107
+00:08:25,060 --> 00:08:28,020
+هل في نقص في بروتين معين، يعني الخلل من عند هذا 
+
+108
+00:08:28,020 --> 00:08:31,780
+البروتين، في شركات معينة مثلا، لما في عندها مواد 
+
+109
+00:08:31,780 --> 00:08:35,740
+تعرف كم تركيز البروتين فيها، أو نسبة البروتين في 
+
+110
+00:08:35,740 --> 00:08:39,260
+الجسم، بدي أعرف كم نسبة البروتين عند هذا الشخص 
+
+111
+00:08:39,260 --> 00:08:44,760
+فإني أقدر كمية البروتين وأعرف كمية البروتين، هذا 
+
+112
+00:08:44,760 --> 00:08:51,990
+إيش مهم، فهي الأهمية من معملنا هنا بنحكي عن الـ 
+
+113
+00:08:51,990 --> 00:08:56,630
+biuret reagent، إذا فاكرين في معمل qualitative test 
+
+114
+00:08:56,630 --> 00:09:00,570
+of amino acid، كان من ضمن الفحوصات فحص الـ biuret 
+
+115
+00:09:00,570 --> 00:09:05,370
+test، الـ biuret test، كنا بنحط المادة على biuret 
+
+116
+00:09:05,370 --> 00:09:10,500
+reagent، إذا اتغير اللون لـ violet، إذا هذا إن هو 
+
+117
+00:09:10,500 --> 00:09:13,480
+positive، إنه البروتين موجود، إنه فيه peptide bond 
+
+118
+00:09:13,480 --> 00:09:16,860
+كنا بس نشوف هل اتغير اللون ولا لأ، إذا اتغير 
+
+119
+00:09:16,860 --> 00:09:20,160
+positive، إذا ما اتغيرش negative، هنا نفس الأشياء 
+
+120
+00:09:20,160 --> 00:09:24,260
+إننا نشتغل على فحص biuret test، ولكن بعد تغير 
+
+121
+00:09:24,260 --> 00:09:27,600
+اللون، بدنا نش، احنا عارفين إنه فيه بروتين بس هذا 
+
+122
+00:09:27,600 --> 00:09:30,800
+اللون بدنا ناخده نجيسه في جهاز ال 
+
+123
+00:09:30,800 --> 00:09:35,570
+spectrophotometer، نجيسه على طول موجي معين عشان 
+
+124
+00:09:35,570 --> 00:09:40,270
+يطلعلي رقم، يطلعلي اللي هو كمية الضوء اللي انتصها 
+
+125
+00:09:40,270 --> 00:09:45,190
+العينة، بيطلعلي رقم، بيطلعلي absorption، انتصاص، جداش 
+
+126
+00:09:45,190 --> 00:09:49,310
+كمية الانتصاص، عشان أعرف تركيز العينة، أنا في الآخر 
+
+127
+00:09:49,310 --> 00:09:54,790
+بدي أطلع بتركيز للعينة، مش بس تغير في لون positive 
+
+128
+00:09:54,790 --> 00:09:58,100
+ولا negative، لأن حكينا بالأول إن اليوم فحصنا 
+
+129
+00:09:58,100 --> 00:10:02,180
+quantitative test of a protein، طب مين من الفحوصات 
+
+130
+00:10:02,180 --> 00:10:05,100
+اللي أخدناها في أول معمل تكشف عن وجود ال protein 
+
+131
+00:10:05,100 --> 00:10:09,560
+هو فحص ال biuret test، أخدناها في أول معمل إنه 
+
+132
+00:10:09,560 --> 00:10:12,460
+بيكشف على وجود الرابط ال peptide bond الموجودة في ال 
+
+133
+00:10:12,460 --> 00:10:16,120
+protein، اليوم إننا نشتغل هذا ال biuret ولكن بعد 
+
+134
+00:10:16,120 --> 00:10:21,300
+ما نشتغله، ناخده ونجيس كمية اللون أو تركيز المادة 
+
+135
+00:10:21,300 --> 00:10:25,340
+بناء على التغير في اللون في جهاز spectrophotometer 
+
+136
+00:10:26,480 --> 00:10:29,900
+إذا فاكرين حكينا إن الـ biuret reagent بيتكون من 
+
+137
+00:10:29,900 --> 00:10:34,720
+أدة مكونات، حكينا إن هو عبارة عن كبريتات نحاس في وجود 
+
+138
+00:10:34,720 --> 00:10:41,500
+alkaline media اللي هي NaOH أو KOH، وفي مكون تالت ما 
+
+139
+00:10:41,500 --> 00:10:45,120
+حكينا عنه اللي هو بوتاسيوم صوديوم تارتريت، لذا الـ 
+
+140
+00:10:45,120 --> 00:10:48,560
+biuret reagent بيتكون من تلت مكونات اللي هم 
+
+141
+00:10:48,560 --> 00:10:52,220
+كبريتات النحاس، بوتاسيوم هيدروكسيد أو صوديوم 
+
+142
+00:10:52,220 --> 00:10:56,880
+هيدروكسيد، وبوتاسيوم صوديوم تارتريت، بيجي الأول 
+
+143
+00:10:56,880 --> 00:11:02,000
+مكون اللي هو كبريتات النحاس، كبريتات النحاس هذا it's this 
+
+144
+00:11:02,000 --> 00:11:06,300
+provides a cupric ion which form chelate complex 
+
+145
+00:11:06,930 --> 00:11:09,530
+والـ cupric ion gives the reagent its 
+
+146
+00:11:09,530 --> 00:11:13,730
+characteristic blue color، إذا كبريتات النحاس وظيفته 
+
+147
+00:11:13,730 --> 00:11:16,630
+ليش موجود في ال reagent عشان يعطيهم ال cupric ion 
+
+148
+00:11:16,630 --> 00:11:21,810
+بيزودني بال cupric ion اللي حكينا إنه هذا أهم 
+
+149
+00:11:21,810 --> 00:11:26,650
+عامل لازم يكون موجود عشان يشتغل كـ chelating agent اللي 
+
+150
+00:11:26,650 --> 00:11:30,730
+هو عامل متمخلب يربط الروابط اللي بيبتديها مع بعضها 
+
+151
+00:11:30,730 --> 00:11:36,770
+لبعض، تكوين chelate complex، إذا فاكرين لما شوفتوا لون 
+
+152
+00:11:36,770 --> 00:11:40,650
+الـ biuret reagent، لونه أزرق، مين أعطاه هذا اللون 
+
+153
+00:11:40,650 --> 00:11:44,010
+هو الـ cupric ion، هو المسؤول عن اللون الأزرق 
+
+154
+00:11:44,010 --> 00:11:49,270
+الموجود في الـ reaction، المكون الثاني، بوتاسيوم 
+
+155
+00:11:49,270 --> 00:11:52,970
+هيدروكسيد أو صوديوم هيدروكسيد، هذا ما بيساهم 
+
+156
+00:11:52,970 --> 00:11:57,150
+بالتفاعل، doesn't participate in the reaction but 
+
+157
+00:11:57,150 --> 00:12:01,530
+provides the alkaline medium، وإنما بيرفع البيئة مش 
+
+158
+00:12:01,530 --> 00:12:06,560
+بس عشان يعطيني alkaline، بيئة في الإعطاء وسط قاعدة 
+
+159
+00:12:06,560 --> 00:12:10,780
+Potassium Sodium تارتريت، المكون الثالث اللي قد 
+
+160
+00:12:10,780 --> 00:12:14,920
+تتواجدوا أول وظيفة Stabilize the chelate complex 
+
+161
+00:12:14,920 --> 00:12:19,140
+بيحافظ على ثباتية ال complex بعد ما يتكون ال 
+
+162
+00:12:19,140 --> 00:12:22,640
+chelate complex، ييجي الكبر يرتبط مع الروابط 
+
+163
+00:12:22,640 --> 00:12:26,420
+البروتينية الموجودة في البروتين ويكون complex اسم 
+
+164
+00:12:26,420 --> 00:12:30,560
+ال chelate اللي هو هذا بيحول اللون من أزرق إلى 
+
+165
+00:12:30,560 --> 00:12:34,800
+violet، إذا positive، اتحول كان لـ violet من blue to 
+
+166
+00:12:34,800 --> 00:12:38,900
+violet، فالميلّي اللي خلّاه يتحول ال cupric ion ارتبط 
+
+167
+00:12:38,900 --> 00:12:43,360
+مع الروابط الببتيدية وكوّن الكيلات كاملة، الـ 
+
+168
+00:12:43,360 --> 00:12:46,680
+Potassium Sodium تارتريت بيحافظ على ثباتية هذا 
+
+169
+00:12:46,680 --> 00:12:49,300
+ال complex، إنه بعد ما يتكون هذا ال complex 
+
+170
+00:12:49,300 --> 00:12:54,020
+ما يتفككش، ما يتكسرش ثاني، يظل متكون، يظل ثابت عشان 
+
+171
+00:12:54,020 --> 00:12:58,140
+أحافظ على اللون وأروح أجيس تركيز 
+
+172
+00:12:58,810 --> 00:13:02,050
+ثاني وظيفة prevent precipitation of copper 
+
+173
+
+201
+00:15:03,080 --> 00:15:06,980
+الرياجنت بيرتبط مع الـ peptide bond بيعمل كلات
+
+202
+00:15:06,980 --> 00:15:11,820
+Complex Formation of Capric Protein Complex اللي 
+
+203
+00:15:11,820 --> 00:15:14,700
+هو الكبر مع البروتين بيعملوله Complex بيحتاج إلى
+
+204
+00:15:14,700 --> 00:15:19,100
+two peptide bonds حكينا إنه بيحتاج إلى رابطتين
+
+205
+00:15:19,100 --> 00:15:23,240
+ببتيديتين على الأقل عشان يعمل ال complex بيتحول
+
+206
+00:15:23,240 --> 00:15:27,290
+اللون إلى violet color كلية البروداكت هذا اللون
+
+207
+00:15:27,290 --> 00:15:30,610
+اللي اتكون ال complex اللي اتكون ولونه violet
+
+208
+00:15:30,610 --> 00:15:35,370
+بيجى ي measured by absorption spectroscopy at 540
+
+209
+00:15:35,370 --> 00:15:40,630
+nanometer بيروح بيجى كمية امتصاص اللون الداكن اللي
+
+210
+00:15:40,630 --> 00:15:44,710
+امتصها العينة على جهاز spectrophotometer على طول
+
+211
+00:15:44,710 --> 00:15:50,740
+موجي 540 nanometer بحكي للـ molecule containing two
+
+212
+00:15:50,740 --> 00:15:53,620
+or more peptide bonds associated with the capric
+
+213
+00:15:53,620 --> 00:16:02,190
+ion to form a complex بيربول قرر تحوي على 
+
+214
+00:16:02,190 --> 00:16:08,790
+رابطتين ببتديتين وأكثر بيرتبط مع الـ Capric Ion
+
+215
+00:16:08,790 --> 00:16:14,070
+وبيعمل لي كمبليكس كلية كمبليكس لونه بيربول بيجى 
+
+216
+00:16:14,070 --> 00:16:19,690
+على خمسمية وأربعين نانو متر في وجود alkaline pH
+
+217
+00:16:19,690 --> 00:16:24,390
+حكينا لازم يكون أن alkaline pH الكبرك ايون الموجود
+
+218
+00:16:24,390 --> 00:16:29,970
+في ال region بيرتبط مع الـ peptide bond بمجرد ما
+
+219
+00:16:29,970 --> 00:16:34,270
+يرتبط بيصير له reduction of من capric ion to
+
+220
+00:16:34,270 --> 00:16:38,930
+caprous ion يعني ملخص الـ principle لأنه هو نفس الـ
+
+221
+00:16:38,930 --> 00:16:42,950
+principle تبع ال bio-test اللي أخدناه في معمل الـ
+
+222
+00:16:42,950 --> 00:16:46,690
+quality test of amino acid أخدنا أن الـ principle
+
+223
+00:16:46,690 --> 00:16:52,080
+حكينا  بناه على شكل معادلة لأن الـ protein زائد copper
+
+224
+00:16:52,080 --> 00:16:58,520
+sulfate في وجود قاعدة اللي هي NAH أو KOH كمان ضفنا
+
+225
+00:16:58,520 --> 00:17:01,880
+عليها اليوم Sodium Potassium Tertarate المكون
+
+226
+00:17:01,880 --> 00:17:07,620
+التالت بيعطينا كلية Complex لون violet هذا الـ
+
+227
+00:17:07,620 --> 00:17:10,300
+принцип اللي أخدناه في أول معمل إيش بده أضيف عليه؟
+
+228
+00:17:10,300 --> 00:17:13,660
+إنّه كلية Complex هذا اللي لون violet  ريد
+
+229
+00:17:13,660 --> 00:17:19,420
+absorption at 540 نانومتر أنا مش بس بدي اشوف
+
+230
+00:17:19,420 --> 00:17:23,940
+اللون لأ بدي اقرأ الامتصاص لهذا اللون عند خمسمية
+
+231
+00:17:23,940 --> 00:17:28,920
+واربعين نانو متر لأنه فحصنا اليوم quantitative بدي
+
+232
+00:17:28,920 --> 00:17:31,720
+اطلع عندي كمية مش qualitative
+
+233
+00:17:34,470 --> 00:17:37,890
+حكينا إنّه في طرقتين تانيات كمان بيعتمدوا على الـ
+
+234
+00:17:37,890 --> 00:17:40,590
+CABAR-Based السائلي حكينا إنّه إحنا عندنا الـ
+
+235
+00:17:40,590 --> 00:17:43,790
+Bioret في عندنا الـ Bicnic Conic Acid وفي عندنا
+
+236
+00:17:43,790 --> 00:17:48,090
+الـ Lowry Method إيش هي ال Lowry Method؟ ال Lowry
+
+237
+00:17:48,090 --> 00:17:52,590
+Method هو فحص بيعتمد على وجود الـ CABAR إنّه فيه
+
+238
+00:17:52,590 --> 00:17:56,690
+Reagent اسمه الـ Falling Reagent نوكابرس ايون
+
+239
+00:17:56,690 --> 00:18:01,810
+detected with فلين كيوكالتيو فلين reagent هذا
+
+240
+00:18:01,810 --> 00:18:06,830
+الفلين reagent منه مكوّنين يتكون من حامضين من
+
+241
+00:18:06,830 --> 00:18:13,390
+فوسفوموليبدك و فوسفوتانجستك أسيد إذا استخدمت فولين
+
+242
+00:18:13,390 --> 00:18:16,430
+رياجانت اللي بيتكون من فوسفوموليبديك أسيد و
+
+243
+00:18:16,430 --> 00:18:20,150
+فوسفوتانجستيك أسيد بنسمي هذه الطريقة الـ Lowry
+
+244
+00:18:20,150 --> 00:18:24,510
+method this method is commonly referred to as the
+
+245
+00:18:24,510 --> 00:18:28,470
+Lowry method كيف هي بتشتغل؟ بتعمل على تحويل زي
+
+246
+00:18:28,470 --> 00:18:33,880
+البروتين بتحول الكابريك أيون إلى كابرس أيون بيعمل
+
+247
+00:18:33,880 --> 00:18:37,780
+كلات مع الرابطة الـ PPD الموجودة في البروتين
+
+248
+00:18:37,780 --> 00:18:42,820
+بيعطيني لون blue Capric ion reduction of following
+
+249
+00:18:42,820 --> 00:18:46,740
+reagent produce a blue color الـ blue color هي
+
+250
+00:18:46,740 --> 00:18:49,640
+اللون الأزرق اللي بيظهر عندي بروح بقرأ له ال
+
+251
+00:18:49,640 --> 00:18:56,280
+absorption بس على طول موجي 650 إلى 750 nanometer
+
+252
+00:18:58,100 --> 00:19:02,160
+بحكي لي كمية اللون اللي ظهرت عندي أو شدة اللون
+
+253
+00:19:02,160 --> 00:19:06,280
+تعتمد على كمية الرابطة البيبتيدية الـ peptide bond
+
+254
+00:19:06,280 --> 00:19:09,980
+amount of peptide bond كل ما زادت الـ peptide bond
+
+255
+00:19:09,980 --> 00:19:15,520
+عدد رابط البيبتيدية أكيد هتزيد عندي شدة اللون يعني
+
+256
+00:19:15,520 --> 00:19:20,000
+هيزيد تركيز المادة لأن كمية البروتين اللي موجودة
+
+257
+00:19:20,000 --> 00:19:25,820
+هيكون أعلى Disadvantage of biuret test في عندنا
+
+258
+00:19:25,820 --> 00:19:29,700
+عيوب لطريقة الـ biuret test الـ biuret test هي
+
+259
+00:19:29,700 --> 00:19:34,340
+طريقة لقياس البروتين في الهيوم أول مشكلة هي إنّه
+
+260
+00:19:34,340 --> 00:19:38,560
+this method required large amount of protein for
+
+261
+00:19:38,560 --> 00:19:43,980
+detection إنّ هاي الطريقة تحتاج إلى كميات كبيرة من
+
+262
+00:19:43,980 --> 00:19:48,920
+البروتين عشان تقدر تكشف عنه يعني هي بتجيس من واحد
+
+263
+00:19:48,920 --> 00:19:53,610
+لعشرين milligram per minute بتجيس كميات البروتين
+
+264
+00:19:53,610 --> 00:19:57,910
+واحد لعشرين إذا كان في العينة في بروتين ولكن أقل
+
+265
+00:19:57,910 --> 00:20:01,950
+من واحد ملي جرام لكل مل بتقدرش هذه الطريقة تجيسه
+
+266
+00:20:01,950 --> 00:20:07,870
+بتعطيني كميات خاطئة إذاً هذه الطريقة بتحتاج لكميات
+
+267
+00:20:07,870 --> 00:20:11,370
+كبيرة من البروتين عشان تقدر تجيسها لو جبت عينة
+
+268
+00:20:11,370 --> 00:20:14,810
+فيها كمية بروتين من واحد لعشرين ميلي جرام لكل مل
+
+269
+00:20:14,810 --> 00:20:19,050
+بتقدر تجيسها لو فيها أقل من واحد، بتقدرش تجيسها
+
+270
+00:20:19,050 --> 00:20:22,730
+إذا العينة فيها بروتين ولكن أقل من واحد ميلي جرام
+
+271
+00:20:22,730 --> 00:20:27,250
+برميل، بتقدرش تجيسها مش هتعطيكي قراءة وهذه بنسميها
+
+272
+00:20:27,250 --> 00:20:33,270
+sensitivity sensitivity تعريفها أقل كمية قادر
+
+273
+00:20:33,270 --> 00:20:39,700
+الفحص على قياسها أقل كمية بيقدر الفحص يجيسها بسميها
+
+274
+00:20:39,700 --> 00:20:43,560
+الـ sensitivity فلو سألت ال sensitivity للفحص ال
+
+275
+00:20:43,560 --> 00:20:49,000
+bio retest  إيداش هي الـ 1 milligram برميل أقل كمية
+
+276
+00:20:49,000 --> 00:20:52,080
+بيقدر ال bio retest يجيسها هي 1 milligram برميل
+
+277
+00:20:52,080 --> 00:20:55,740
+إذا ال sensitivity لل bio retest هي 1 milligram
+
+278
+00:20:55,740 --> 00:21:00,820
+برميل تعتبر ال sensitivity له قليلة أول عيوب هذه
+
+279
+00:21:00,820 --> 00:21:06,030
+الطريقة أن ال sensitivity له قليلة تاني من عيوبه إنّ
+
+280
+00:21:06,030 --> 00:21:09,530
+هي sensitive to a variety of nitrogen-containing
+
+281
+00:21:09,530 --> 00:21:15,590
+substances إنّ هذه طريقة حساسة لأي مركب كيميائي
+
+282
+00:21:15,590 --> 00:21:21,150
+يحتوي على رابطتين ببتديتين فالكبرك ايون هو بيتمد
+
+283
+00:21:21,150 --> 00:21:25,450
+على وجود رابطة ببتدية تنتن في أكتر بيجى في الـ
+
+284
+00:21:25,450 --> 00:21:28,730
+Cupric Ion اللي موجود في الرياجنت ويرتبط مع هذه
+
+285
+00:21:28,730 --> 00:21:33,430
+الرابطة البيبتدية وبيكوّن كلات Complex اللي لون
+
+286
+00:21:33,430 --> 00:21:38,800
+violet وبيجى يسه على طول موجي 540 نانومتر الرابطتين
+
+287
+00:21:38,800 --> 00:21:42,360
+البيبتيديتين هدول موجودين في البروتين بحط على
+
+288
+00:21:42,360 --> 00:21:46,980
+البروتين bio-retraction وبيجى ي كمية الكلية ال
+
+289
+00:21:46,980 --> 00:21:50,040
+complex اللي اتكون وعطاني اللون بيجى يشدّة هذا اللون
+
+290
+00:21:50,040 --> 00:21:55,080
+في الجهات ممكن في مركبات تانية تحتوي على رابطتين
+
+291
+00:21:55,080 --> 00:21:59,140
+بيبتيديتين فعلاً زي مين؟ زي المركب اسمه الـ Bioria
+
+292
+00:21:59,140 --> 00:22:03,360
+بيوريا معناه إنّ مركبين يوريا بيرتبطوا مع بعض
+
+293
+00:22:03,360 --> 00:22:07,930
+بيقدروا يرتبطوا مع بعض بيتكون بينهم رابطتين
+
+294
+00:22:07,930 --> 00:22:12,270
+بيبتديتين فبما إنّ هذا المركب خلصار فيه رابطتين
+
+295
+00:22:12,270 --> 00:22:16,930
+بيبتديتين إذا أن العينة إذا فيها بيوريا بيوريا هذه
+
+296
+00:22:16,930 --> 00:22:20,630
+فيها رابطتين بيبتديتين حطيت عليها بيوري تريجين
+
+297
+00:22:20,630 --> 00:22:25,110
+بيجي الكبر كعادي بيرتبط مع الرابطة البيبتدية بيكون
+
+298
+00:22:25,110 --> 00:22:29,350
+كلات Complex لونه violet بيجى يشدّة اللون هذا على
+
+299
+00:22:29,350 --> 00:22:33,650
+540 نانومتر وبيعطيني قراءة طب هذه القراءة اللي طلعت
+
+300
+00:22:33,650 --> 00:22:37,670
+معايا مش هتكون لبروتين هتكون لمركب اسمه الـ Bioria
+
+301
+00:22:37,670 --> 00:22:41,630
+مابدهنش يعرفني هل هي للـ Bioria ولا للبروتين يعني
+
+302
+00:22:41,630 --> 00:22:47,060
+ممكن تعطيني نتائج خاطئة إنّه لحساسة لوجود مركبات
+
+303
+00:22:47,060 --> 00:22:51,560
+أخرى غير البروتين يعني هذه الطريقة مش خاصة بس
+
+304
+00:22:51,560 --> 00:22:55,140
+للبروتين إنّها تعطيني قراءة بس للبروتين not
+
+305
+00:22:55,140 --> 00:23:00,120
+specific، specificity عندها قليلة إنّها مش خاصة بس
+
+306
+00:23:00,120 --> 00:23:04,620
+للبروتين وإنّما لأي مركب يحتوي على رابطتين
+
+307
+00:23:04,620 --> 00:23:10,530
+ببتديتين فأكتر زي البيوريا إذنّ طريق العيوب الـ
+
+308
+00:23:10,530 --> 00:23:14,370
+Biorhythm test إنّه ال sensitivity له قليلة و ال
+
+309
+00:23:14,370 --> 00:23:19,370
+specificity له قليلة أقل كمية قادرة على قياسها
+
+310
+00:23:19,370 --> 00:23:23,850
+قليلة ال specificity الخصوصية له برضه قليلة إنّه
+
+311
+00:23:23,850 --> 00:23:29,950
+ممكن تختلط مواد أخرى و يعطيني إليها قراءة إيش هو
+
+312
+00:23:29,950 --> 00:23:33,530
+ال standard curve؟ ال standard curve هو عبارة عن
+
+313
+00:23:33,530 --> 00:23:37,190
+type of graph used for quantitative research
+
+314
+00:23:37,190 --> 00:23:43,540
+technique هو رسم بياني لمعرفة تركيز أي مادة مجهولة
+
+315
+00:23:43,540 --> 00:23:48,680
+رسم بياني برسمه عشان أعرف تركيز أي مادة مجهولة
+
+316
+00:23:48,680 --> 00:23:52,380
+standard care for protein concentration is often
+
+317
+00:23:52,380 --> 00:23:56,520
+created using non-concentration of bovine serum
+
+318
+00:23:56,520 --> 00:24:00,320
+إنّه كيف أنا بدي استخدم هذا الرسم البياني لمعرفة
+
+319
+00:24:00,320 --> 00:24:04,120
+تركيز أي مادة مجهولة باستخدام حاجة اسمها standard
+
+320
+00:24:04,120 --> 00:24:09,430
+مرجع اللي هو عبارة عن البروتين بيكون معروف التركيز
+
+321
+00:24:09,430 --> 00:24:15,490
+هذا البروتين بيكون موجود في الجسم البروتين اللي
+
+322
+00:24:15,490 --> 00:24:22,150
+هنستخدمه هو الـ bovine serum
+
+323
+00:24:22,150 --> 00:24:28,910
+albumin وبروتين الألبومين من عجل صغير الألبومين هو
+
+324
+00:24:28,910 --> 00:24:33,210
+عبارة عن سيرام البروتين اللي بينقل الفاتي أسيد
+
+325
+00:24:33,210 --> 00:24:42,310
+ومهم في تحفظ بلازمة BH هو بروتين موجود في الدم
+
+326
+00:24:42,310 --> 00:24:48,070
+يعمل كناقل لبعض المواد زي الفاتي أسيد ويحافظ على
+
+327
+00:24:48,070 --> 00:24:53,940
+الـ BH في الـ Protein Quantization Assay بوفاين
+
+328
+00:24:53,940 --> 00:24:58,900
+سيرام ألبومين تعمل كـ Reference Protein تستخدم
+
+329
+00:24:58,900 --> 00:25:04,060
+لإصلاح الـ Protein Standard Camp في فحوصات لمعرفة
+
+330
+00:25:04,060 --> 00:25:08,400
+تركيز البروتين نستخدم البروتين للـ Bovine Serum
+
+331
+00:25:08,400 --> 00:25:13,860
+البروتين كبروتين مرجع كستاندر كمان ده بتكون عارفين
+
+332
+00:25:13,860 --> 00:25:18,540
+تركيزها ومن خلالها بتعرف تركيز العينة اللي موجودة
+
+333
+00:25:18,540 --> 00:25:24,690
+عندها هي مادة معروفة التركيز بستخدمها كمرجع لتقدير
+
+334
+00:25:24,690 --> 00:25:30,350
+تركيز مادة مجهولة الـ standard solution هو non
+
+335
+00:25:30,350 --> 00:25:34,330
+-concentration of sample الـ standard هو مادة
+
+336
+00:25:34,330 --> 00:25:40,910
+بتكون معروفة التركيز، مادة أنا عارفة تركيزها الـ
+
+337
+00:25:40,910 --> 00:25:44,050
+standard curve حكى هنا إنّه هو رسم بياني لمعرفة
+
+338
+00:25:44,050 --> 00:25:48,790
+تركيز أي مادة مجهولة بناء على إيش، بناء على وجود
+
+339
+00:25:48,790 --> 00:25:54,870
+standard مادة معروفة التركيز فبيجى أنا ضروري أرسم
+
+340
+00:25:54,870 --> 00:25:58,170
+للـStandard Curve عشان أعرف التركيز العين المجهولة
+
+341
+00:25:58,170 --> 00:26:01,450
+اللي بتكون عندي كيف بأبدأ أعرف هذا التركيز؟ بناء على
+
+342
+00:26:01,450 --> 00:26:05,990
+وجود مادة معروف التركيز، مادة كمرجع المادة هذه
+
+343
+00:26:05,990 --> 00:26:09,410
+المعروفة التركيز اسمها الـStandard بتيجي من
+
+344
+00:26:09,410 --> 00:26:13,310
+الشركة هي بتيجيها من الشركة بيكون معروف تركيزها
+
+345
+00:26:13,310 --> 00:26:17,190
+مكتوب عليها إنّه هو الـStandard وتركيزها هالجان إمّا
+
+346
+00:26:17,190 --> 00:26:21,350
+بتيجي من الشركة مخففة، من هذا الـ standard نفسه
+
+347
+00:26:21,350 --> 00:26:25,450
+مخفف ومعمول منه عدة تخفيفات، كل تخفيف له تركيز
+
+348
+00:26:25,450 --> 00:26:28,850
+مختلف، مثلًا الأولى بيكون مكتوب عليها إنّها هي 5
+
+349
+00:26:28,850 --> 00:26:32,570
+milligram per meal، تانية 10 milligram per meal،
+
+350
+00:26:32,570 --> 00:26:36,510
+25 milligram per meal، 50 milligram per meal، إنّه
+
+351
+00:26:36,510 --> 00:26:41,210
+مخفف وجاهز أو بتيجي standard مركز مثلًا مكتوب عليه
+
+352
+00:26:41,210 --> 00:26:44,510
+إنّه هو 2 milligram per ml وإحنا مطلوب منّا إنّ إحنا
+
+353
+00:26:44,510 --> 00:26:49,270
+نعمل منه عدة تخفيفات عشان نشتغل عليه لو أنا عملت
+
+354
+00:26:49,270 --> 00:26:53,150
+عدة تخفيفات كيف بدي احسب التخفيف في الـ dilution
+
+355
+00:26:53,150 --> 00:27:02,430
+بالقانون C1V1 بتساوي C2V2 اللي هو التركيز الأول تركيز
+
+356
+00:27:02,430 --> 00:27:05,890
+ال standard كده كان جابته لي الشركة إنّه 2 milligram
+
+357
+00:27:05,890 --> 00:27:10,210
+في ال volume ضرب ال volume كده حجم اللي أنا أخدته
+
+358
+00:27:10,210 --> 00:27:14,310
+من هذا ال standard بيساوي C2 التركيز اللي بعد ما
+
+359
+00:27:14,310 --> 00:27:17,860
+خففته هذا اللي أنا بتاعه عارفه مجهول ضرب الـ Volume
+
+360
+00:27:17,860 --> 00:27:22,220
+2 اللي هو أد إيش أنا أخدت من الـ Distilled water و
+
+361
+00:27:22,220 --> 00:27:24,760
+أد إيش أخدت من الـ Standard يعني الـ Volume الـ
+
+362
+00:27:24,760 --> 00:27:28,260
+Total، الكلي اللي هو حاجة أد إيش أنا أخدت من الـ
+
+363
+00:27:28,260 --> 00:27:32,840
+Standard وأد إيش أضفت عليه Distilled water، الـ
+
+364
+00:27:32,840 --> 00:27:35,960
+Volume الكلي هذا هيوضح معنا إن شاء الله في المعمل
+
+365
+00:27:35,960 --> 00:27:38,540
+هنوضح كيف هذا القانون
+
+366
+00:27:40,920 --> 00:27:43,600
+How to calculate the concentration كيف نحسب 
+
+367
+00:27:43,600 --> 00:27:47,460
+التركيز حكى هنا بناء على قانون الـPierre Lambert's
+
+368
+00:27:47,460 --> 00:27:52,680
+law اللي بيحكي إنّه في علاقة بين تركيز المادة وكمية
+
+369
+00:27:52,680 --> 00:27:56,240
+الضوء الممتص إنّ الـconcentration of substance
+
+370
+00:27:56,240 --> 00:27:59,080
+directly proportional to the amount of light
+
+371
+00:27:59,080 --> 00:28:03,440
+absorbed إنّه تركيز المادة بيتناسب تناسب طردي مع
+
+372
+00:28:03,440 --> 00:28:07,840
+كمية الضوء الممتص أهم نقطة هي النقطة الأخيرة في
+
+373
+00:28:07,840 --> 00:28:11,360
+هاي ال slide إنّه ال reference range for total
+
+374
+00:28:11,360 --> 00:28:18,540
+protein كمية البروت
+
+401
+00:30:08,540 --> 00:30:12,640
+spectrophotometer وبقرأ الـ absorption ال
+
+402
+00:30:12,640 --> 00:30:16,400
+absorption على فرض الأولى طلعت point واحد اثنين
+
+403
+00:30:16,400 --> 00:30:21,160
+ثلاثة تمام بطلعها باخد الثانية اللي هي تركيزها عشر
+
+404
+00:30:21,160 --> 00:30:24,860
+بقرأ الـ absorption على فرض point واحد خمسة أربعة
+
+405
+00:30:25,570 --> 00:30:30,090
+باخد 25 لتركيزها 25 وبقرأ لها الـ absorption على
+
+406
+00:30:30,090 --> 00:30:35,490
+فرض point 2 3 5 بعدين باخد لتركيزها 50 وبقرأ أعلى
+
+407
+00:30:35,490 --> 00:30:41,130
+فرض point 3 3 تمام؟ هالجهة أنا بيصير عندي معروف
+
+408
+00:30:41,130 --> 00:30:44,690
+تركيز لهذه المادة وأخدتها قرأتها في جهاز
+
+409
+00:30:44,690 --> 00:30:47,310
+Spectrophotometer فصار عندي معروف اللي هي
+
+410
+00:30:47,310 --> 00:30:51,130
+absorption إذا عندي معروف تركيز، concentration و
+
+411
+00:30:51,130 --> 00:30:55,830
+absorption بجي برسم الـ Standard Curve الـ Standard
+
+412
+00:30:55,830 --> 00:31:00,850
+Curve كيف بدي أرسمه؟ بعبارة عن تقاطع سيني مع صعدي
+
+413
+00:31:00,850 --> 00:31:06,710
+على الخط السيني بيمثل الـ concentration والخط الصعدي 
+
+414
+00:31:06,710 --> 00:31:10,790
+يمثل الـ absorption is a standard curve الرسم
+
+415
+00:31:10,790 --> 00:31:15,630
+وعبارة عن تقاطع سيني وصعدي على الخط السيني بحط ال
+
+416
+00:31:15,630 --> 00:31:20,090
+concentration وعلى الصعدي بحط الـ absorption بحط
+
+417
+00:31:20,090 --> 00:31:25,210
+تقسيمة لخط السيني وخط الصعدي وبجيب أرسم مثلا أول
+
+418
+00:31:25,210 --> 00:31:27,870
+نقطة اللي هي خمسة من السيني من الـ concentration
+
+419
+00:31:27,870 --> 00:31:31,910
+تركيزها كان خمسة مع تقاطع من الـ absorption طلعت
+
+420
+00:31:31,910 --> 00:31:35,510
+point واحد اثنين ثلاثة بحط النقطة الأولى اللي هي
+
+421
+00:31:35,510 --> 00:31:38,890
+تقاطع خمسة مع point واحد اثنين ثلاثة النقطة
+
+422
+00:31:38,890 --> 00:31:41,730
+الثانية اللي هي تركيزها عشرة مع point واحد خمسة
+
+423
+00:31:41,730 --> 00:31:46,430
+أربعة بحط النقطة الثالثة خمسة وعشرين تركيزها من ال
+
+424
+00:31:46,430 --> 00:31:51,200
+concentration مع point اثنين ثلاثة خمسة الرابعة
+
+425
+00:31:51,200 --> 00:31:56,540
+تركيزها الخمسين مع point ثلاثة ثلاثة بحط النقطة
+
+426
+00:31:56,540 --> 00:32:01,300
+وبعدين بوصل النقاط بأكبر عدد من النقاط بحيث يمثل خط
+
+427
+00:32:01,300 --> 00:32:07,000
+مستقيم برسم خط مستقيم يمر بأكبر عدد من النقاط بوصل
+
+428
+00:32:07,000 --> 00:32:12,300
+النقاط مع بعض على بعض بحيث يمثل خط مستقيم بتكون
+
+429
+00:32:12,300 --> 00:32:18,280
+عندي من ضمن هدول في عندي عينة مجهولة التركيز في
+
+430
+00:32:18,280 --> 00:32:21,480
+عندي عينة ماخدينها من مريض مثلًا لأعرف تركيز
+
+431
+00:32:21,480 --> 00:32:24,720
+البروتين فيها بحط عليها الـ Reagent وباخدها على
+
+432
+00:32:24,720 --> 00:32:28,760
+جهاز الـ Spectrophotometer بقرا إليها absorption،
+
+433
+00:32:28,760 --> 00:32:32,360
+انتصار في هذه المادة بيصير عندي معروف إليها
+
+434
+00:32:32,360 --> 00:32:37,360
+الـ absorption بس مش معروف التركيز باجي على التقاط
+
+435
+00:32:37,360 --> 00:32:40,940
+عصاد اللي بيمثل الـ absorption على فرض العينة مثلًا
+
+436
+00:32:40,940 --> 00:32:47,990
+طلعت point 1.7 بأجي على point 1.7 من الـ absorption
+
+437
+00:32:47,990 --> 00:32:53,230
+بوصل خط اللي وصولا إلى الخط المستقيم اللي في الوسط
+
+438
+00:32:53,230 --> 00:32:58,910
+وبنزل عمود على الخط الساني وبشوف جداش .. وين وين
+
+439
+00:32:58,910 --> 00:33:03,430
+وصلت عندي تركيز وبكتب تركيز هذه العينة المجهولة
+
+440
+00:33:04,260 --> 00:33:09,080
+أنا عارف إنه صعب عليكم تفهموه هنا لكن هنوضحه في
+
+441
+00:33:09,080 --> 00:33:11,880
+المعمل ونرسمه إن شاء الله على الصجورة ونوضح كيف
+
+442
+00:33:11,880 --> 00:33:17,810
+بينعمل الـ Standard Curve شايفين هنا الرسم اللي تحت
+
+443
+00:33:17,810 --> 00:33:22,110
+اللي هو تقاطع سيني مع صعدي خط السيني بيمثل الـ
+
+444
+00:33:22,110 --> 00:33:25,790
+concentration والخط الصعدي بيمثل الـ absorption
+
+445
+00:33:25,790 --> 00:33:30,350
+يكون عندي مواد جاني معروف تركيزها كل واحدة إلاها
+
+446
+00:33:30,350 --> 00:33:34,030
+concentration مختلف و absorption على الخط الصعدي
+
+447
+00:33:34,030 --> 00:33:36,890
+أنا أخذت هذه العينات وقرأتها في جهاز
+
+448
+00:33:36,890 --> 00:33:40,860
+Spectrophotometer وطلع معايا absorption هتبقى شيء
+
+449
+00:33:40,860 --> 00:33:45,160
+بمثل أول نقطة مثلًا على فرقة خمسة من السيني ال
+
+450
+00:33:45,160 --> 00:33:48,300
+concentration ومن الصادر طلع الـ absorption لها
+
+451
+00:33:48,300 --> 00:33:52,800
+point واحد اثنين بقى بحط نقطة النقطة اللي بعدها لكل
+
+452
+00:33:52,800 --> 00:33:56,360
+عينة جداش تركيزها ماجداش الـ absorption بحط النقطة
+
+453
+00:33:56,360 --> 00:34:02,940
+بوصل خط مستقيم يمر بمعظم النقاط في عندي عينة بتكون
+
+454
+00:34:02,940 --> 00:34:07,600
+unknown عينة مثلًا ماخدها من مريض وفيها بروتين بدي
+
+455
+00:34:07,600 --> 00:34:10,920
+أعرف كمية هذه البروتين بحط عليها من الـ reagent زي
+
+456
+00:34:10,920 --> 00:34:14,700
+الـ standard بعملها معاملة الـ standard بحط عليها
+
+457
+00:34:14,700 --> 00:34:17,020
+reagent وبعدين باخدها على جهاز الـ
+
+458
+00:34:17,020 --> 00:34:20,580
+spectrophotometer وفي الجهاز بيعطيني absorption
+
+459
+00:34:20,580 --> 00:34:24,800
+بيعطيني امتصاص باجي على الخط الصعدي اللي هي ال
+
+460
+00:34:24,800 --> 00:34:27,500
+absorption أنا عارفة الـ absorption لهذه العينة
+
+461
+00:34:27,500 --> 00:34:31,720
+قرأتها على الجهاز وعلى فرض طلعت point 1 3 5 يعني
+
+462
+00:34:31,720 --> 00:34:36,270
+ده كلمة signal الخط الأحمر على فرض هي أنها طلعت الـ
+
+463
+00:34:36,270 --> 00:34:39,790
+absorption بوصل خط إلى الخط المستقيم اللي في
+
+464
+00:34:39,790 --> 00:34:43,670
+المنتصف وبسقط عمود من عند النقطة اللي وصلت عندها
+
+465
+00:34:43,670 --> 00:34:48,790
+بسقط عمود على الصانع وإن ما سقط هذا العمود بيكون
+
+466
+00:34:48,790 --> 00:34:53,570
+هذا الـ concentration هذا عبارة عن تركيز هذه المادة
+
+467
+00:34:53,570 --> 00:34:57,430
+عبارة طلعت مثلًا بين خمسين وخمسة وعشرين بقدر إنه
+
+468
+00:34:57,430 --> 00:35:01,750
+ممكن تكون هي خمسة وأربعين ممكن تكون خمسة وثلاثين
+
+469
+00:35:01,750 --> 00:35:08,330
+فشوف وين وقعت بالظبط إذا هذا هو تركيز المادة وهي
+
+470
+00:35:08,330 --> 00:35:13,190
+عندنا هنا مخطط ثاني على الخط السيني بحط الـ
+
+471
+00:35:13,190 --> 00:35:16,250
+Concentration وهنا على الخط الصعدي بحط الـ
+
+472
+00:35:16,250 --> 00:35:21,270
+Absorption كل عينة أنا قرأتها جداش إلاها absorption
+
+473
+00:35:21,270 --> 00:35:25,410
+مع جداش concentration بحط نقطة، النقطة اللي بعلى،
+
+474
+00:35:25,410 --> 00:35:31,050
+بعدين بحط خط مستقيم بيمر بمعظم النقاط، والعينة
+
+475
+00:35:31,050 --> 00:35:35,890
+المجهولة بشوف عندها جداش هي الـ absorption بكون أنا
+
+476
+00:35:35,890 --> 00:35:38,370
+قرأها على الجهاز وعارفة إيه لها الـ absorption
+
+477
+00:35:38,370 --> 00:35:43,030
+جداش الامتصاص طلع بوصل على الخط المستقيم وبسقط
+
+478
+00:35:43,030 --> 00:35:47,150
+عمودًا على الـ concentration وبشوف وين يطلع تركيزها
+
+479
+00:35:47,150 --> 00:35:52,250
+عند أي رقم هيك بكون أنا عارفة تركيز هذه المادة
+
+480
+00:35:52,250 --> 00:35:57,390
+وبرضه هنرجع نوضح في المعمل كيف المخطط نرسمه وكيف
+
+481
+00:35:57,390 --> 00:36:05,170
+نعمل على إيجاد تركيز أي مادة مجهولة هنا عندنا مجموع
+
+482
+00:36:05,170 --> 00:36:09,550
+من التعريفات عندنا تعريف الـ sensitivity حكينا
+
+483
+00:36:09,550 --> 00:36:14,390
+عنها sensitivity هي أقل كمية أو أقل تركيز من
+
+484
+00:36:14,390 --> 00:36:19,190
+الأنالايت قادر لفحصها لقياسه أقل كمية بيقدر يقيسها
+
+485
+00:36:19,190 --> 00:36:22,990
+بنسميها sensitivity lowest amount of analyte in
+
+486
+00:36:22,990 --> 00:36:27,110
+sample which can be detected أما الـ specificity
+
+487
+00:36:27,110 --> 00:36:31,990
+اللي هي الخصوصية الخصوصية هي قدرة الفحص إنه يكون
+
+488
+00:36:31,990 --> 00:36:36,730
+خاص أو فريد فقط لـ Analyte معين اللي أنا ببحث عنه
+
+489
+00:36:36,730 --> 00:36:41,710
+is the ability to assess uniquely vocally the
+
+490
+00:36:41,710 --> 00:36:45,490
+analyte in the presence of components which may be
+
+491
+00:36:45,490 --> 00:36:49,150
+expected to be present إنه عندي مثلًا عينة أنا عندي
+
+492
+00:36:49,150 --> 00:36:52,720
+هذا الفحص بدي فحص السكر طب هي العينة اللي أنا أخدتها
+
+493
+00:36:52,720 --> 00:36:56,780
+فيها عدة مكونات من ضمنها السكر لكن فيه حاجات ثانية
+
+494
+00:36:56,780 --> 00:37:01,080
+فيه مثلًا البروتين وفيه carbohydrate سكر لكن أنا
+
+495
+00:37:01,080 --> 00:37:04,600
+هذا الفحص إذا كان عنده specificity عالية فهو بس
+
+496
+00:37:04,600 --> 00:37:08,600
+قادر على قياس السكر ما لوش دخل في المكونات الثانية
+
+497
+00:37:08,600 --> 00:37:13,520
+ما يقيسنيش غير السكر بس قدرة الفحص على أنه يرتبط مع
+
+498
+00:37:13,520 --> 00:37:17,240
+الأنالايت اللي أنا ببحث عنه مع حاجة واحدة بدون
+
+499
+00:37:17,240 --> 00:37:22,040
+التداخلات مع المكونات الأخرى الـ Linearity
+
+500
+00:37:22,040 --> 00:37:37,000
+Linearity of Analytical Procedure هي علاقة
+
+501
+00:37:37,000 --> 00:37:40,600
+الترضية اللي حكينا عنها بالـ absorption و الـ
+
+502
+00:37:40,600 --> 00:37:43,990
+concentration هي الـ directly proportional اللي هي
+
+503
+00:37:43,990 --> 00:37:48,450
+العلاقة الخطية معناها العلاقة الترضية اللي بين
+
+504
+00:37:48,450 --> 00:37:55,150
+تركيز المادة وكمية الضوء اللي بيمتصها إيه شغلنا
+
+505
+00:37:55,150 --> 00:37:59,270
+لليوم؟ اليوم هو فحصنا الـ virus test لنشتغل virus
+
+506
+00:37:59,270 --> 00:38:03,370
+test ولكن كـ quantitative اللي بدي اقرأه في جهاز
+
+507
+00:38:03,370 --> 00:38:06,990
+spectrophotometer وبالنهاية بدي أعمل standard
+
+508
+00:38:06,990 --> 00:38:10,330
+curve بس اعمل هذا الـ standard curve
+
+509
+00:38:15,570 --> 00:38:26,610
+تركيز المادة المجهولة تركيز
+
+510
+00:38:26,610 --> 00:38:27,770
+المادة المجهولة تركيز المادة المجهولة تركيز المادة
+
+511
+00:38:27,770 --> 00:38:32,350
+المجهولة تركيز المادة المجهولة تركيز المادة
+
+512
+00:38:32,350 --> 00:38:40,390
+المجهولة تركيز المادة المجهولة تركيز المادة
+
+513
+00:38:40,390 --> 00:38:46,400
+المجهولة فكيف بدي أخففه؟ بنعمل من هذا الـ Standard
+
+514
+00:38:46,400 --> 00:38:51,700
+6 تيوبات بحكي للتيوب الأول، احنا بشو بنخفف؟ بنحط
+
+515
+00:38:51,700 --> 00:38:54,600
+من المادة المركزة، بنخففها في الـ distilled water
+
+516
+00:38:54,600 --> 00:38:57,860
+فبحكي للتيوب الأول، حطي عليه من الـ distilled
+
+517
+00:38:57,860 --> 00:39:02,560
+water خمسة من عشرة مل فلجيت احنا بشو بنستخدم لما
+
+518
+00:39:02,560 --> 00:39:08,420
+نحط؟ بنستخدم بايبت والبايبت هي بالمايكرون كل واحد
+
+519
+00:39:08,420 --> 00:39:14,710
+مل بيعطيني ألف مايكرون يعني الخمسة مل تعطيني خمسمائة
+
+520
+00:39:14,710 --> 00:39:18,710
+مايكرون كل هدول الأرقام اللي بالجدول بالمل بتحولهم
+
+521
+00:39:18,710 --> 00:39:24,450
+لمايكرون كاب كل واحد مل بيعطيني ألف مايكرون خمسة
+
+522
+00:39:24,450 --> 00:39:28,370
+من عشرة مل يعني خمسمائة مايكرون تطلع فالـ tube
+
+523
+00:39:28,370 --> 00:39:32,890
+الأول بحكي لي نحط عليها خمسمائة مايكرون من الـ
+
+524
+00:39:32,890 --> 00:39:36,130
+distilled water وبدناش نحط عليها من الـ protein
+
+525
+00:39:36,130 --> 00:39:40,960
+standard، نحط صفر طب أنا ما حطتش الـ protein خالص
+
+526
+00:39:40,960 --> 00:39:43,560
+ما جداش تركيز الـ protein في الـ tube الأول
+
+527
+00:39:43,560 --> 00:39:49,200
+concentration له صفر أكيد صفر أنا عندي الـ tube
+
+528
+00:39:49,200 --> 00:39:53,600
+الأول بس حقيته عليه distilled water ما حطيتش عليه من
+
+529
+00:39:53,600 --> 00:39:59,600
+الـ standard فإذا تركيزه هيكون صفر ما فيش تركيز له
+
+530
+00:39:59,600 --> 00:40:05,240
+صفر milligram per ml لأنه ما حطناش عليه ولا شيء من
+
+531
+00:40:05,240 --> 00:40:10,030
+الـ standard "تيوب" رقم 2 بدي أحط عليها من الـ
+
+532
+00:40:10,030 --> 00:40:14,730
+"دستيل واتر" 4 من 10 مل يعني 400 مايكرون ومن الـ
+
+533
+00:40:14,730 --> 00:40:20,690
+"بروتين ستاندرد" بدي أحط عليها 100 مايكرون 1 من 10
+
+534
+00:40:20,690 --> 00:40:24,350
+مل يعني 100 مايكرون لذلك هذه خففة الـ "ستاندرد"
+
+535
+00:40:24,350 --> 00:40:28,270
+الأول إن حطيت 4 من 10 "دستيل واتر" يعني 400
+
+536
+00:40:28,270 --> 00:40:32,450
+مايكرون على الـ "ستاندرد" الـ "بروتين ستاندرد" 1
+
+537
+00:40:32,450 --> 00:40:38,760
+من 10 مل يعني 100 مايكرون قداش تركيزه بيطلع بعد
+
+538
+00:40:38,760 --> 00:40:42,500
+تخفيف 4 من 10؟ كيف حسبتي التركيز؟ ركزوا معايا فيها
+
+539
+00:40:42,500 --> 00:40:47,880
+حكينا بنحسب تركيز الـ Standard المخفف بناء على
+
+540
+00:40:47,880 --> 00:40:55,340
+قانون C1 V1 سواء C2 V2 خلينا في تيوب رقم 2 ركزوا
+
+541
+00:40:55,340 --> 00:41:00,140
+معايا على تيوب رقم 2 كيف بده يحسب التركيز؟ C1 اللي
+
+542
+00:41:00,140 --> 00:41:04,160
+هو التركيز للـ Standard قبل التخفيف احنا حكينا إنه
+
+543
+00:41:04,160 --> 00:41:07,360
+جاينا من الشركة الـ Standard جداش تركيزه 2
+
+544
+00:41:07,360 --> 00:41:13,740
+milligram per ml، إذا C1 هي 2 milligram per ml درب V1
+
+545
+00:41:13,740 --> 00:41:18,800
+الـ V1 هذا قداش أنا أخدت من الـ Standard قداش حطيت
+
+546
+00:41:18,800 --> 00:41:21,080
+على تيوب اثنين من الـ Standard البروتين الـ
+
+547
+00:41:21,080 --> 00:41:24,920
+Standard حكي لي واحد من عشرة مل يعني مائة مايكرو
+
+548
+00:41:24,920 --> 00:41:29,580
+إذاً C1 اللي هو تركيزه الأصلي تركيز الـ Standard
+
+549
+00:41:29,580 --> 00:41:36,320
+قداش كان عندي اثنين درب V1 V1 هي كمية الحجم اللي
+
+550
+00:41:36,320 --> 00:41:39,940
+أنا أخدته من الـ Standard قداش أخدت مائة إذا صار
+
+551
+00:41:39,940 --> 00:41:45,800
+عندي اثنين درب مائة بساوي C2، إيش هي C2؟ هو التركيز
+
+552
+00:41:45,800 --> 00:41:49,120
+اللي أنا بتعرفه، قداش صارت بعد، قداش تركيزها
+
+553
+00:41:49,120 --> 00:41:53,680
+بعد، فهي وسيبنا مجهولة، لسه بديها، V2 هو الـ
+
+554
+00:41:53,680 --> 00:41:57,020
+Total، الحجم كله، قداش حطيت من الـ Distilled
+
+555
+00:41:57,020 --> 00:42:00,640
+water مع الـ Standard، في Tube 2، قداش حطيت، حكي
+
+556
+00:42:00,640 --> 00:42:03,420
+أنا حطينا من الـ Distilled water، 4 من 10 ميلي،
+
+557
+00:42:03,420 --> 00:42:07,550
+يعني 400 مايكرو من الـ Standard حطيت 1 من 10 مل
+
+558
+00:42:07,550 --> 00:42:10,950
+يعني 100 مايكرون يعني 400 مايكرون و 100 مايكرون
+
+559
+00:42:10,950 --> 00:42:16,550
+يعني الـ total كله قداش 500 مايكرون، إذا الـ V2 هي
+
+560
+00:42:16,550 --> 00:42:21,550
+500 مايكرون، إذا صرت بعرف C1 2 عبارة عن 2 ميلي
+
+561
+00:42:21,550 --> 00:42:28,990
+جرام، V1 هي 100 مايكرون، C2 بديها، V2 هي 500
+
+562
+00:42:28,990 --> 00:42:34,730
+مايكرون من المعادلة 2 ضرب 100 على 500 بتطلع التركيز
+
+563
+00:42:34,730 --> 00:42:39,530
+4 من 10 فهمنا كيف جبنا الـ Concentration لـ Tube
+
+564
+00:42:39,530 --> 00:42:47,410
+2 من القانون C1 V1 سواء C2 V2 منهي C1 هو تركيز الـ
+
+565
+00:42:47,410 --> 00:42:50,830
+Standard اللي بدون تخفيف الأصل اللي جانب الشركة
+
+566
+00:42:50,830 --> 00:42:56,050
+قداش كان 2 milligram per ml الـ V1 هي التركيز
+
+567
+00:42:56,050 --> 00:42:58,570
+اللي أنا أخدته من الـ Standard قداش أخدتي حجم من
+
+568
+00:42:58,570 --> 00:43:03,250
+الـ Standard؟ أخذنا 100 مايكرو الـ C2 هذا بديها
+
+569
+00:43:03,250 --> 00:43:07,390
+مجهولة الـ V2 هي عبارة عن الحجم كله distilled water
+
+570
+00:43:07,390 --> 00:43:11,970
+زائد للـ Standard أخدت 400 مع 100 يعني 500 مايكرو
+
+571
+00:43:11,970 --> 00:43:16,690
+بيطلع عند
+
+601
+00:45:25,740 --> 00:45:29,960
+العينة اللي جاية عندي تحط خمسة من عشرة مل، يعني
+
+602
+00:45:29,960 --> 00:45:35,240
+خمسمية مايكروليتر أنا عارف كمية تركيزها، كمية
+
+603
+00:45:35,240 --> 00:45:39,740
+البروتين لهذا المريض؟ لأ، بنحكي لبعد ما نحضر هدول
+
+604
+00:45:39,740 --> 00:45:43,840
+التيوبات كلهم بنحط واحد مل من الـ bioretary agent
+
+605
+00:45:43,840 --> 00:45:48,860
+على كل التيوبات بنحط واحد مل من الـ bioretary 
+
+606
+00:45:48,860 --> 00:45:53,940
+agent على كل التيوبات و أعمل mix بعدين بتعملها 
+
+607
+00:45:53,940 --> 00:45:59,900
+incubation لمدة عشرين دقيقة، تخليها مدة عشرين دقيقة
+
+608
+00:45:59,900 --> 00:46:06,340
+على الـ room تنبرجها عشان يصير ارتفاع، بعدين بدي أخد
+
+609
+00:46:06,340 --> 00:46:09,920
+كل تيوب من هذه التيوبات و أقرأها على جهاز
+
+610
+00:46:09,920 --> 00:46:14,780
+Spectrophotometer على طول موجي خمسمية و أربعين
+
+611
+00:46:14,780 --> 00:46:19,260
+نانومتر، إيش هيطلعلي؟ هيطلعلي absorbance، يعني كل
+
+612
+00:46:19,260 --> 00:46:23,710
+تيوب بحطها بيعطيني قراءة absorption، الـ tube اللي
+
+613
+00:46:23,710 --> 00:46:28,550
+بعدها بيعطيني absorption وهكذا للسبعة tubes، فصار
+
+614
+00:46:28,550 --> 00:46:31,510
+عندي لل tubes من واحد لستة معروف الـ
+
+615
+00:46:31,510 --> 00:46:35,550
+concentration ومعروف عندي absorption، بس ال tube
+
+616
+00:46:35,550 --> 00:46:39,790
+رقم سبعة ال unknown بس معروف عندي absorption، قرأت
+
+617
+00:46:39,790 --> 00:46:43,410
+على جهاز الـ spectrophotometer وعرفت ال absorption
+
+618
+00:46:43,410 --> 00:46:50,350
+ماقدرتش أعرف ال concentration، تمام هالجيت بعمل ال
+
+619
+00:46:50,350 --> 00:46:53,530
+standard curve، برسم ال curve اللي هو عبارة عن
+
+620
+00:46:53,530 --> 00:46:57,450
+مضحنة حكاية سيني وصادي، على الخط السيني بدي أرسم ال
+
+621
+00:46:57,450 --> 00:47:01,550
+concentration وعلى الصادي بدي أرسم ال absorption
+
+622
+00:47:02,360 --> 00:47:07,940
+بحط النقاط لـ Standard و بعدين بطلّع تركيز المادة
+
+623
+00:47:07,940 --> 00:47:11,640
+المجهولة، أنا عارفة إيه لها absorption و بدي أطلّع
+
+624
+00:47:11,640 --> 00:47:15,440
+تركيزها من الـ standard gap، إن شاء الله هذا كله
+
+625
+00:47:15,440 --> 00:47:21,460
+هيوضح معناه بالشغل و لما نوضحها بالمعنى بيحكيلي في
+
+626
+00:47:21,460 --> 00:47:25,820
+حاجة اسمها blank solution، هو solution محلول بيحتوي
+
+627
+00:47:25,820 --> 00:47:29,140
+على little to no alkaline، usually used to
+
+628
+00:47:29,140 --> 00:47:33,060
+calibrate an instrument، إنه هذا هو عبارة عن محلول،
+
+629
+00:47:33,060 --> 00:47:36,480
+بحطش فيه ولا أي شيء، ولا standard ولا sample، بس
+
+630
+00:47:36,480 --> 00:47:40,560
+بحط فيه الـreagent عشان الـreagent إيه اللون، إذا
+
+631
+00:47:40,560 --> 00:47:44,200
+تبين الـbio-reagent، لونه أزرق فجيت أنا لو هذا
+
+632
+00:47:44,200 --> 00:47:47,460
+اللون أنا حاططه على جهاز الـSpectrum، هيقرألي هذا
+
+633
+00:47:47,460 --> 00:47:52,960
+اللون الأزرق، فهي يتلخبط ويعطيني قراءة زيادة من
+
+634
+00:47:52,960 --> 00:47:56,520
+اللون الأزرق، الـ Reagent نفسه واللون بتاع التفاعل
+
+635
+00:47:56,520 --> 00:48:00,260
+مش الجهاز اللي بيقرأ كمية اللون، فهيقرأ لون الـ
+
+636
+00:48:00,260 --> 00:48:05,920
+Reagent  العصني وهيقرأ لون التفاعل، فأنا بحط الـ
+
+637
+00:48:05,920 --> 00:48:09,720
+tube بس تحتوي على الـ Reagent عشان أصفر عليها
+
+638
+00:48:09,720 --> 00:48:14,020
+الجهاز، إن الجهاز يقرأ كمية هذا اللون و بعد هي
+
+639
+00:48:14,020 --> 00:48:17,980
+يخصومها كأنه بده يخصومها من التيوبات التانية زي
+
+640
+00:48:17,980 --> 00:48:21,300
+إذا خدتوا في المقدمة كيف نوزن على الميزان كيف
+
+641
+00:48:21,300 --> 00:48:25,540
+بنستخدم الميزان، بحط ال cup مثلا اللي بدي أوزن فيه
+
+642
+00:48:25,540 --> 00:48:28,660
+بدي أحط فيه المادة، أول أجي بحط ال cup عشان إيه
+
+643
+00:48:28,660 --> 00:48:34,740
+الوزن بصفر عليه و بعدين بحط المادة عشان يشيل وزنة
+
+644
+00:48:34,740 --> 00:48:40,800
+ال cup، وأنا بصفر على لون الـ reagent عشان بعد هيك
+
+645
+00:48:40,800 --> 00:48:46,240
+أشيل لون الـ reagent عشان يعملني calibration، فالمرة
+
+646
+00:48:46,240 --> 00:48:50,620
+الجاي لما نشتغل هذه التجربة بدنا معانا ورق رسم
+
+647
+00:48:50,620 --> 00:48:55,260
+بياني عشان نرسم عليه الـ standard curve
diff --git a/PL9fwy3NUQKwZ1Q7zOg1ObgufPMjRI3WY1/tYZzyB0o5Ps_raw.json b/PL9fwy3NUQKwZ1Q7zOg1ObgufPMjRI3WY1/tYZzyB0o5Ps_raw.json
new file mode 100644
index 0000000000000000000000000000000000000000..e770ebaf73067a18c54c096461970e6bdfb72502
--- /dev/null
+++ b/PL9fwy3NUQKwZ1Q7zOg1ObgufPMjRI3WY1/tYZzyB0o5Ps_raw.json
@@ -0,0 +1 @@
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"probability": 0.862060546875}, {"start": 200.31, "end": 200.97, "word": " طرق", "probability": 0.986328125}, {"start": 200.97, "end": 201.45, "word": " في", "probability": 0.6962890625}, {"start": 201.45, "end": 201.65, "word": " أن", "probability": 0.93310546875}, {"start": 201.65, "end": 201.99, "word": " أحد", "probability": 0.934814453125}, {"start": 201.99, "end": 202.45, "word": " الطرق", "probability": 0.9899088541666666}, {"start": 202.45, "end": 202.65, "word": " هي", "probability": 0.966796875}, {"start": 202.65, "end": 202.89, "word": " الـ", "probability": 0.74560546875}, {"start": 202.89, "end": 204.11, "word": " spectrophotometry", "probability": 0.8408203125}, {"start": 204.11, "end": 204.67, "word": " based", "probability": 0.875}, {"start": 204.67, "end": 205.07, "word": " on", "probability": 0.95361328125}, {"start": 205.07, "end": 205.57, "word": " UV", "probability": 0.96923828125}, {"start": 205.57, "end": 205.91, "word": " on", "probability": 0.338134765625}, 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تقليدية وقديمة البروتين اكسيرت Ultraviolet-Light في 280 نانومتر", "tokens": [3555, 1863, 16606, 15844, 37893, 45761, 2288, 6156, 2407, 11242, 2407, 7578, 8717, 36178, 7435, 10632, 20925, 23566, 302, 12, 43, 1607, 39896, 23032, 16572, 28671, 6055, 4587, 20292, 3215, 10632, 4032, 28543, 32640, 3660, 2423, 26890, 35473, 9957, 1975, 4117, 3794, 13546, 2655, 20925, 23566, 302, 12, 43, 397, 8978, 41229, 8717, 7649, 20498, 2655, 2288], "avg_logprob": -0.42726294131114567, "compression_ratio": 1.221476510067114, "no_speech_prob": 7.569789886474609e-06, "words": [{"start": 212.45, "end": 213.43, "word": "بناء", "probability": 0.7340494791666666}, {"start": 213.43, "end": 213.75, "word": " على", "probability": 0.7470703125}, {"start": 213.75, "end": 214.73, "word": " مشاعر", "probability": 0.694580078125}, {"start": 214.73, "end": 215.13, "word": " فوضوى", "probability": 0.635009765625}, {"start": 215.13, "end": 215.83, "word": " نفسجية", "probability": 0.8897705078125}, {"start": 215.83, "end": 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reaction المكون التاني بوتاسيوم هيدروكسيد أو صوديوم هيدروكسيد هذا ما بيساهم بالتفاعل doesn't participate in the reaction but provides the alkaline medium وإنما بيرفع البيئة مش بس عشان يعطيني alkaline", "tokens": [28814, 15730, 6156, 995, 37983, 9957, 5296, 15042, 13412, 38688, 2655, 14407, 5296, 11536, 2423, 39184, 3228, 26262, 822, 9461, 5296, 11536, 3224, 5551, 11622, 2288, 4587, 3714, 9957, 5551, 3615, 9566, 40294, 23758, 13672, 11536, 31439, 2423, 39184, 1410, 1341, 17437, 31439, 9673, 3794, 33604, 12610, 18871, 13672, 11536, 16247, 11622, 2288, 4587, 9673, 29245, 23328, 8978, 2423, 39184, 5480, 9673, 30544, 16712, 7649, 1829, 4724, 35473, 32277, 1829, 20498, 8032, 25708, 32887, 4117, 3794, 25708, 34051, 20328, 2407, 16254, 20498, 8032, 25708, 32887, 4117, 3794, 25708, 23758, 19446, 4724, 1829, 3794, 995, 16095, 20666, 2655, 5172, 995, 30241, 1177, 380, 8197, 294, 264, 5480, 457, 6417, 264, 44220, 533, 6399, 4032, 28814, 1863, 15042, 4724, 13546, 5172, 3615, 2423, 21292, 19986, 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{"id": 35, "seek": 88937, "start": 860.69, "end": 889.37, "text": "اللي هو الـ Potassium Sodium Tertiary فعنا تلت وظائف للـ Potassium Sodium Tertiary إيش ال principle اللي عملنا اليوم ال principle أنه أنا بدي أجيس كمية ال protein determining total protein in sample is the biorhythm method by biorhythm method أن أنا بدي أجيس كمية البروتين الموجودة في العينة بطريقة ال biorhythm method", "tokens": [6027, 20292, 31439, 2423, 39184, 9145, 27609, 42059, 2197, 314, 911, 29104, 6156, 3615, 8315, 6055, 1211, 2655, 4032, 19913, 16373, 5172, 24976, 39184, 9145, 27609, 42059, 2197, 314, 911, 29104, 11933, 1829, 8592, 2423, 8665, 13672, 1829, 6225, 42213, 8315, 45595, 20498, 2423, 8665, 14739, 3224, 41850, 4724, 16254, 5551, 7435, 1829, 3794, 9122, 2304, 10632, 2423, 7944, 23751, 3217, 7944, 294, 6889, 307, 264, 272, 1973, 34494, 3170, 538, 272, 1973, 34494, 3170, 14739, 41850, 4724, 16254, 5551, 7435, 1829, 3794, 9122, 2304, 10632, 2423, 26890, 35473, 9957, 9673, 29245, 23328, 3660, 8978, 18863, 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559, 317, 24525, 2304, 3224, 2423, 39184, 7465, 278, 1300, 559, 317], "avg_logprob": -0.2853822215529513, "compression_ratio": 1.811659192825112, "no_speech_prob": 6.496906280517578e-06, "words": [{"start": 1054.47, "end": 1054.91, "word": "حكينا", "probability": 0.9075927734375}, {"start": 1054.91, "end": 1055.13, "word": " إنه", "probability": 0.5546875}, {"start": 1055.13, "end": 1055.25, "word": " في", "probability": 0.89306640625}, {"start": 1055.25, "end": 1056.23, "word": " طرقتين", "probability": 0.8414306640625}, {"start": 1056.23, "end": 1056.63, "word": " تانيات", "probability": 0.83990478515625}, {"start": 1056.63, "end": 1056.89, "word": " كمان", "probability": 0.8196614583333334}, {"start": 1056.89, "end": 1057.51, "word": " بيعتمدوا", "probability": 0.930908203125}, {"start": 1057.51, "end": 1057.65, "word": " على", "probability": 0.8974609375}, {"start": 1057.65, "end": 1057.89, "word": " الـ", "probability": 0.79833984375}, {"start": 1057.89, "end": 1058.21, "word": " 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"end": 1062.49, "word": " Bicnic", "probability": 0.34912109375}, {"start": 1062.49, "end": 1062.79, "word": " Conic", "probability": 0.8291015625}, {"start": 1062.79, "end": 1063.15, "word": " Acid", "probability": 0.910400390625}, {"start": 1063.15, "end": 1063.43, "word": " وفي", "probability": 0.83349609375}, {"start": 1063.43, "end": 1063.79, "word": " عندنا", "probability": 0.975341796875}, {"start": 1063.79, "end": 1064.11, "word": " الـ", "probability": 0.928466796875}, {"start": 1064.11, "end": 1064.41, "word": " Lowry", "probability": 0.3154296875}, {"start": 1064.41, "end": 1064.89, "word": " Method", "probability": 0.469970703125}, {"start": 1064.89, "end": 1065.85, "word": " إيش", "probability": 0.8826497395833334}, {"start": 1065.85, "end": 1066.07, "word": " هي", "probability": 0.95947265625}, {"start": 1066.07, "end": 1066.21, "word": " ال", "probability": 0.86181640625}, {"start": 1066.21, "end": 1066.51, "word": " Lowry", "probability": 0.850341796875}, {"start": 1066.51, "end": 1067.53, "word": " Method؟", "probability": 0.734619140625}, {"start": 1067.53, "end": 1067.75, "word": " ال", "probability": 0.94287109375}, {"start": 1067.75, "end": 1068.09, "word": " Lowry", "probability": 0.824951171875}, {"start": 1068.09, "end": 1068.59, "word": " Method", "probability": 0.79833984375}, {"start": 1068.59, "end": 1069.37, "word": " هو", "probability": 0.9599609375}, {"start": 1069.37, "end": 1070.07, "word": " فحص", "probability": 0.95703125}, {"start": 1070.07, "end": 1071.03, "word": " بيعتمد", "probability": 0.973828125}, {"start": 1071.03, "end": 1071.17, "word": " على", "probability": 0.94775390625}, {"start": 1071.17, "end": 1071.49, "word": " وجود", "probability": 0.98876953125}, {"start": 1071.49, "end": 1071.71, "word": " الـ", "probability": 0.665283203125}, {"start": 1071.71, "end": 1072.05, "word": " CABAR", "probability": 0.8006998697916666}, {"start": 1072.05, "end": 1072.37, "word": " إنه", "probability": 0.833740234375}, {"start": 1072.37, "end": 1072.59, "word": " فيه", "probability": 0.92578125}, {"start": 1072.59, "end": 1073.15, "word": " Reagent", "probability": 0.7373860677083334}, {"start": 1073.15, "end": 1073.69, "word": " اسمه", "probability": 0.9265950520833334}, {"start": 1073.69, "end": 1073.97, "word": " الـ", "probability": 0.894287109375}, {"start": 1073.97, "end": 1074.35, "word": " Falling", "probability": 0.805419921875}, {"start": 1074.35, "end": 1075.25, "word": " Reagent", "probability": 0.9401041666666666}], "temperature": 1.0}, {"id": 45, "seek": 108989, "start": 1075.47, "end": 1089.89, "text": "نوكابرس ايون detected with فلين كيوكالتيو فلين reagent هذا الفلين reagent منه مكوّنين يتكون من حشتين من فوسفوموليبدك و فوسفوتانجستك أسن", "tokens": [1863, 2407, 4117, 16758, 2288, 3794, 1975, 1829, 11536, 1141, 39963, 365, 6156, 1211, 9957, 9122, 1829, 2407, 4117, 6027, 2655, 1829, 2407, 6156, 1211, 9957, 26949, 317, 23758, 27188, 1211, 9957, 26949, 317, 9154, 3224, 3714, 4117, 2407, 11703, 1863, 9957, 7251, 2655, 30544, 9154, 11331, 8592, 2655, 9957, 9154, 6156, 41779, 5172, 20498, 12610, 1829, 3555, 3215, 4117, 4032, 6156, 41779, 5172, 35473, 7649, 7435, 14851, 4117, 5551, 3794, 1863], "avg_logprob": -0.42808220484485365, "compression_ratio": 1.5342465753424657, "no_speech_prob": 1.1920928955078125e-07, "words": [{"start": 1075.47, "end": 1076.27, "word": "نوكابرس", "probability": 0.6059672037760416}, {"start": 1076.27, "end": 1076.69, "word": " ايون", "probability": 0.7285970052083334}, {"start": 1076.69, "end": 1077.27, "word": " detected", "probability": 0.478515625}, {"start": 1077.27, "end": 1077.59, "word": " with", "probability": 0.8740234375}, {"start": 1077.59, "end": 1078.09, "word": " فلين", "probability": 0.4181925455729167}, {"start": 1078.09, "end": 1079.65, "word": " كيوكالتيو", "probability": 0.8564453125}, {"start": 1079.65, "end": 1080.67, "word": " فلين", "probability": 0.6712646484375}, {"start": 1080.67, "end": 1081.23, "word": " reagent", "probability": 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فوسفوتانجستك", "probability": 0.7772216796875}, {"start": 1089.51, "end": 1089.89, "word": " أسن", "probability": 0.7288411458333334}], "temperature": 1.0}, {"id": 46, "seek": 111207, "start": 1091.61, "end": 1112.07, "text": "إذا استخدمت فولين رياجانت اللي بيتكون من فوسفوموليبديك أسد و فوسفوتانجستيك أسن بنسمي هذه الطريقة الـ Lowry method this method is commonly referred to as the Lowry method كيف هي بتشتغل؟ بتعمل على تحويل زي البييروت بتحول كابريك أيون إلى كابرس أيون", "tokens": [28814, 15730, 44713, 9778, 40448, 2655, 6156, 12610, 9957, 12602, 1829, 26108, 7649, 2655, 13672, 1829, 4724, 36081, 30544, 9154, 6156, 41779, 5172, 20498, 12610, 1829, 3555, 16254, 4117, 5551, 3794, 3215, 4032, 6156, 41779, 5172, 35473, 7649, 7435, 14851, 1829, 4117, 5551, 3794, 1863, 44945, 38251, 1829, 29538, 41950, 16572, 28671, 2423, 39184, 17078, 627, 3170, 341, 3170, 307, 12719, 10839, 281, 382, 264, 17078, 627, 3170, 9122, 33911, 39896, 39894, 8592, 2655, 17082, 1211, 22807, 39894, 25957, 1211, 15844, 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في البروتين بيعطيني لون blue Capric ion reduction of following reagent produce a blue color الـ blue color هي اللون الأزرق اللي بيظهر عندي بروح بقرأ له ال absorption بس على طول موجي 650 إلى 750 nanometer", "tokens": [21292, 25957, 1211, 28242, 9307, 20449, 34892, 16758, 9566, 2423, 39184, 430, 17349, 9673, 29245, 23328, 3660, 8978, 2423, 26890, 35473, 9957, 4724, 40228, 9566, 1829, 22653, 5296, 11536, 3344, 8363, 1341, 17437, 11004, 295, 3480, 26949, 317, 5258, 257, 3344, 2017, 2423, 39184, 3344, 2017, 39896, 13672, 11536, 16247, 11622, 2288, 4587, 13672, 1829, 4724, 1829, 19913, 3224, 2288, 18871, 16254, 4724, 32887, 5016, 4724, 4587, 2288, 10721, 46740, 2423, 27557, 4724, 3794, 15844, 23032, 12610, 3714, 29245, 1829, 38566, 30731, 31682, 14067, 13606], "avg_logprob": -0.2463662754311118, "compression_ratio": 1.4743589743589745, "no_speech_prob": 3.814697265625e-06, "words": [{"start": 1113.34, "end": 1113.88, "word": "بيعمل", "probability": 0.9171549479166666}, {"start": 1113.88, 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0.9775390625}, {"start": 1131.14, "end": 1131.36, "word": " على", "probability": 0.92333984375}, {"start": 1131.36, "end": 1131.66, "word": " طول", "probability": 0.99609375}, {"start": 1131.66, "end": 1132.24, "word": " موجي", "probability": 0.9560546875}, {"start": 1132.24, "end": 1133.66, "word": " 650", "probability": 0.78173828125}, {"start": 1133.66, "end": 1133.98, "word": " إلى", "probability": 0.77294921875}, {"start": 1133.98, "end": 1135.12, "word": " 750", "probability": 0.9453125}, {"start": 1135.12, "end": 1136.28, "word": " nanometer", "probability": 0.637451171875}], "temperature": 1.0}, {"id": 48, "seek": 116098, "start": 1138.1, "end": 1160.98, "text": "بحكيلي كمية اللون اللى زهرت عندى او شدة اللون تعتمد على كمية الرابطة البيبتيدية الـ peptide bond amount of peptide bond كل ما زادت الـ peptide bond عدد رابط البيبتيدية اكيد هتزيد عندى شدة اللون يعني هيزيد تركيز المادة لان كمية البروتين اللى موجوده هيكون اعلى", "tokens": [49628, 4117, 1829, 20292, 9122, 2304, 10632, 13672, 11536, 13672, 7578, 30767, 3224, 43500, 43242, 7578, 1975, 2407, 13412, 41891, 13672, 11536, 6055, 34268, 2304, 3215, 15844, 9122, 2304, 10632, 34892, 16758, 9566, 3660, 2423, 21292, 3555, 31371, 3215, 10632, 2423, 39184, 41781, 482, 6086, 2372, 295, 41781, 482, 6086, 28242, 19446, 30767, 18513, 2655, 2423, 39184, 41781, 482, 6086, 6225, 3215, 3215, 12602, 16758, 9566, 2423, 21292, 3555, 31371, 3215, 10632, 1975, 4117, 25708, 8032, 2655, 11622, 25708, 43242, 7578, 13412, 41891, 13672, 11536, 37495, 22653, 39896, 11622, 25708, 6055, 31747, 1829, 11622, 9673, 18513, 3660, 5296, 7649, 9122, 2304, 10632, 2423, 26890, 35473, 9957, 13672, 7578, 3714, 29245, 23328, 3224, 39896, 30544, 1975, 3615, 23942], "avg_logprob": -0.16710804642762167, "compression_ratio": 2.0673076923076925, "no_speech_prob": 1.4483928680419922e-05, "words": [{"start": 1138.1, "end": 1138.8, "word": "بحكيلي", "probability": 0.8271484375}, {"start": 1138.8, "end": 1139.34, "word": " كمية", "probability": 0.9816080729166666}, {"start": 1139.34, "end": 1139.92, "word": " اللون", "probability": 0.98046875}, {"start": 1139.92, "end": 1140.18, "word": " اللى", "probability": 0.76416015625}, {"start": 1140.18, "end": 1140.6, "word": " زهرت", "probability": 0.8984375}, {"start": 1140.6, "end": 1141.06, "word": " عندى", "probability": 0.794189453125}, {"start": 1141.06, "end": 1141.24, "word": " او", "probability": 0.772216796875}, {"start": 1141.24, "end": 1141.58, "word": " شدة", "probability": 0.7093505859375}, {"start": 1141.58, "end": 1142.16, "word": " اللون", "probability": 0.995849609375}, {"start": 1142.16, "end": 1143.16, "word": " تعتمد", "probability": 0.869140625}, {"start": 1143.16, "end": 1143.42, "word": " على", "probability": 0.78076171875}, {"start": 1143.42, "end": 1144.08, "word": " كمية", "probability": 0.994140625}, {"start": 1144.08, "end": 1144.72, "word": " الرابطة", "probability": 0.919677734375}, {"start": 1144.72, "end": 1145.38, "word": " البيبتيدية", "probability": 0.7163899739583334}, {"start": 1145.38, "end": 1145.56, "word": " الـ", "probability": 0.55413818359375}, {"start": 1145.56, "end": 1146.02, "word": " peptide", "probability": 0.797607421875}, {"start": 1146.02, "end": 1146.28, "word": " bond", "probability": 0.83837890625}, {"start": 1146.28, "end": 1146.68, "word": " amount", "probability": 0.763671875}, {"start": 1146.68, "end": 1146.9, "word": " of", "probability": 0.8857421875}, {"start": 1146.9, "end": 1147.42, "word": " peptide", "probability": 0.949951171875}, {"start": 1147.42, "end": 1147.66, "word": " bond", "probability": 0.9267578125}, {"start": 1147.66, "end": 1148.42, "word": " كل", "probability": 0.896484375}, {"start": 1148.42, "end": 1148.66, "word": " ما", "probability": 0.654296875}, {"start": 1148.66, "end": 1149.16, "word": " زادت", "probability": 0.9855143229166666}, {"start": 1149.16, "end": 1149.34, "word": " الـ", "probability": 0.7030029296875}, {"start": 1149.34, "end": 1149.76, "word": " peptide", "probability": 0.906494140625}, {"start": 1149.76, "end": 1149.98, "word": " bond", "probability": 0.953125}, {"start": 1149.98, "end": 1150.34, "word": " عدد", "probability": 0.9554036458333334}, {"start": 1150.34, "end": 1150.7, "word": " رابط", "probability": 0.873046875}, {"start": 1150.7, "end": 1151.32, "word": " البيبتيدية", "probability": 0.8802897135416666}, {"start": 1151.32, "end": 1151.98, "word": " اكيد", "probability": 0.8312174479166666}, {"start": 1151.98, "end": 1152.94, "word": " هتزيد", "probability": 0.98779296875}, {"start": 1152.94, "end": 1153.52, "word": " عندى", "probability": 0.88720703125}, {"start": 1153.52, "end": 1154.18, "word": " شدة", "probability": 0.9140625}, {"start": 1154.18, "end": 1154.68, "word": " اللون", "probability": 0.996826171875}, {"start": 1154.68, "end": 1155.52, "word": " يعني", "probability": 0.716796875}, {"start": 1155.52, "end": 1156.2, "word": " هيزيد", "probability": 0.865234375}, {"start": 1156.2, "end": 1157.08, "word": " تركيز", "probability": 0.9915771484375}, {"start": 1157.08, "end": 1158.02, "word": " المادة", "probability": 0.984375}, {"start": 1158.02, "end": 1158.24, "word": " لان", "probability": 0.4765625}, {"start": 1158.24, "end": 1158.72, "word": " كمية", "probability": 0.9920247395833334}, {"start": 1158.72, "end": 1159.3, "word": " البروتين", "probability": 0.9456787109375}, {"start": 1159.3, "end": 1159.44, "word": " اللى", "probability": 0.83251953125}, {"start": 1159.44, "end": 1160.0, "word": " موجوده", "probability": 0.8603515625}, {"start": 1160.0, "end": 1160.46, "word": " هيكون", "probability": 0.834228515625}, {"start": 1160.46, "end": 1160.98, "word": " اعلى", "probability": 0.9029947916666666}], "temperature": 1.0}, {"id": 49, "seek": 119106, "start": 1163.14, "end": 1191.06, "text": "Disadvantage of biuret test في عندنا عيوب لطريقة الـ biuret test الـ biuret test هي طريقة لقياس البروتين في الهيوم أول مشكلة هي إنه this method required large amount of protein for detection إن هاي الطريقة تحتاج إلى كميات كبيرة من البروتين عشان تقدر تكشفانه يعني هي بتجيس من واحد لعشرين milligram per minute", "tokens": [38742, 345, 23408, 295, 3228, 540, 83, 1500, 8978, 43242, 8315, 6225, 1829, 37746, 5296, 9566, 16572, 28671, 2423, 39184, 3228, 540, 83, 1500, 2423, 39184, 3228, 540, 83, 1500, 39896, 23032, 16572, 28671, 5296, 38436, 32277, 2423, 26890, 35473, 9957, 8978, 2423, 3224, 1829, 20498, 5551, 12610, 37893, 28820, 3660, 39896, 36145, 3224, 341, 3170, 4739, 2416, 2372, 295, 7944, 337, 17784, 36145, 8032, 47302, 41950, 16572, 28671, 6055, 33753, 26108, 30731, 9122, 2304, 1829, 9307, 9122, 3555, 48923, 9154, 2423, 26890, 35473, 9957, 6225, 8592, 7649, 6055, 28543, 2288, 6055, 4117, 8592, 5172, 7649, 3224, 37495, 22653, 39896, 39894, 7435, 1829, 3794, 9154, 36764, 24401, 5296, 3615, 46309, 9957, 38298, 680, 3456], "avg_logprob": -0.1944293447162794, "compression_ratio": 1.6386861313868613, "no_speech_prob": 5.0067901611328125e-06, "words": [{"start": 1163.1399999999999, "end": 1164.1, "word": "Disadvantage", "probability": 0.6474609375}, {"start": 1164.1, "end": 1164.44, "word": " of", "probability": 0.9501953125}, {"start": 1164.44, "end": 1164.92, "word": " biuret", "probability": 0.5404459635416666}, {"start": 1164.92, "end": 1165.22, "word": " test", "probability": 0.60400390625}, {"start": 1165.22, "end": 1165.5, "word": " في", "probability": 0.40185546875}, {"start": 1165.5, "end": 1165.82, "word": " عندنا", "probability": 0.72998046875}, {"start": 1165.82, "end": 1166.38, "word": " عيوب", "probability": 0.9803059895833334}, {"start": 1166.38, "end": 1167.14, "word": " لطريقة", "probability": 0.980224609375}, {"start": 1167.14, "end": 1167.44, "word": " الـ", "probability": 0.6824951171875}, {"start": 1167.44, "end": 1168.0, "word": " biuret", "probability": 0.8401692708333334}, {"start": 1168.0, "end": 1168.4, "word": " test", "probability": 0.9208984375}, {"start": 1168.4, "end": 1169.1, "word": " الـ", "probability": 0.51806640625}, {"start": 1169.1, "end": 1169.38, "word": " biuret", "probability": 0.9562174479166666}, {"start": 1169.38, "end": 1169.58, "word": " test", "probability": 0.9169921875}, {"start": 1169.58, "end": 1169.7, "word": " هي", "probability": 0.97265625}, {"start": 1169.7, "end": 1170.04, "word": " طريقة", "probability": 0.9788411458333334}, {"start": 1170.04, "end": 1170.52, "word": " لقياس", "probability": 0.7923177083333334}, {"start": 1170.52, "end": 1171.16, "word": " البروتين", "probability": 0.8822021484375}, {"start": 1171.16, "end": 1171.38, "word": " في", "probability": 0.9736328125}, {"start": 1171.38, "end": 1171.88, "word": " الهيوم", "probability": 0.79833984375}, {"start": 1171.88, "end": 1173.04, "word": " أول", "probability": 0.948486328125}, {"start": 1173.04, "end": 1173.74, "word": " مشكلة", "probability": 0.9739583333333334}, {"start": 1173.74, "end": 1173.96, "word": " هي", "probability": 0.873046875}, {"start": 1173.96, "end": 1174.34, "word": " إنه", "probability": 0.3958740234375}, {"start": 1174.34, "end": 1174.54, "word": " this", "probability": 0.35107421875}, {"start": 1174.54, "end": 1174.94, "word": " method", "probability": 0.9755859375}, {"start": 1174.94, "end": 1175.34, "word": " required", "probability": 0.83642578125}, {"start": 1175.34, "end": 1176.18, "word": " large", "probability": 0.73974609375}, {"start": 1176.18, "end": 1177.02, "word": " amount", "probability": 0.9345703125}, {"start": 1177.02, "end": 1177.8, "word": " of", "probability": 0.94677734375}, {"start": 1177.8, "end": 1178.3, "word": " protein", "probability": 0.736328125}, {"start": 1178.3, "end": 1178.56, "word": " for", "probability": 0.94677734375}, {"start": 1178.56, "end": 1179.08, "word": " detection", "probability": 0.93994140625}, {"start": 1179.08, "end": 1179.52, "word": " إن", "probability": 0.5751953125}, {"start": 1179.52, "end": 1179.98, "word": " هاي", "probability": 0.5765380859375}, {"start": 1179.98, "end": 1180.76, "word": " الطريقة", "probability": 0.9920247395833334}, {"start": 1180.76, "end": 1182.12, "word": " تحتاج", "probability": 0.9357096354166666}, {"start": 1182.12, "end": 1182.36, "word": " إلى", "probability": 0.9140625}, {"start": 1182.36, "end": 1183.14, "word": " كميات", "probability": 0.98388671875}, {"start": 1183.14, "end": 1183.72, "word": " كبيرة", "probability": 0.9905598958333334}, {"start": 1183.72, "end": 1183.98, "word": " من", "probability": 0.9931640625}, {"start": 1183.98, "end": 1184.64, "word": " البروتين", "probability": 0.9764404296875}, {"start": 1184.64, "end": 1185.0, "word": " عشان", "probability": 0.970703125}, {"start": 1185.0, "end": 1185.54, "word": " تقدر", "probability": 0.9796549479166666}, {"start": 1185.54, "end": 1186.58, "word": " تكشفانه", "probability": 0.914794921875}, {"start": 1186.58, "end": 1187.42, "word": " يعني", "probability": 0.91943359375}, {"start": 1187.42, "end": 1187.64, "word": " هي", "probability": 0.85888671875}, {"start": 1187.64, "end": 1188.24, "word": " بتجيس", "probability": 0.8717041015625}, {"start": 1188.24, "end": 1188.44, "word": " من", "probability": 0.98974609375}, {"start": 1188.44, "end": 1188.92, "word": " واحد", "probability": 0.849365234375}, {"start": 1188.92, "end": 1189.66, "word": " لعشرين", "probability": 0.9375}, {"start": 1189.66, "end": 1190.18, "word": " milligram", "probability": 0.7119140625}, {"start": 1190.18, "end": 1190.76, "word": " per", "probability": 0.8759765625}, {"start": 1190.76, "end": 1191.06, "word": " minute", "probability": 0.3310546875}], "temperature": 1.0}, {"id": 50, "seek": 120445, "start": 1191.71, "end": 1204.45, "text": "بتجيس كميات البروتين واحد لعشرين إذا كان في العينة في بروتين ولكن أقل من واحد ملي جرام لكل ميل بتقدرش هذه الطريقة تجيسه بتعطيني كميات خاطر", "tokens": [3555, 2655, 7435, 1829, 3794, 9122, 2304, 1829, 9307, 2423, 26890, 35473, 9957, 36764, 24401, 5296, 3615, 46309, 9957, 11933, 15730, 25961, 8978, 18863, 9957, 3660, 8978, 4724, 32887, 2655, 9957, 35525, 19452, 5551, 4587, 1211, 9154, 36764, 24401, 3714, 20292, 10874, 2288, 10943, 5296, 28820, 3714, 26895, 39894, 28543, 2288, 8592, 29538, 41950, 16572, 28671, 6055, 7435, 1829, 3794, 3224, 39894, 3615, 9566, 9957, 1829, 9122, 2304, 1829, 9307, 16490, 41193, 2288], "avg_logprob": -0.15540540057259636, "compression_ratio": 1.7430555555555556, "no_speech_prob": 1.0609626770019531e-05, "words": [{"start": 1191.71, "end": 1192.27, "word": "بتجيس", "probability": 0.9421875}, {"start": 1192.27, "end": 1192.91, "word": " كميات", "probability": 0.9791259765625}, {"start": 1192.91, "end": 1193.61, "word": " البروتين", "probability": 0.868408203125}, {"start": 1193.61, "end": 1194.13, "word": " واحد", "probability": 0.60150146484375}, {"start": 1194.13, "end": 1194.75, "word": " لعشرين", "probability": 0.8878173828125}, {"start": 1194.75, "end": 1195.47, "word": " إذا", "probability": 0.764404296875}, {"start": 1195.47, "end": 1195.77, "word": " كان", "probability": 0.9912109375}, {"start": 1195.77, "end": 1195.91, "word": " في", "probability": 0.96240234375}, {"start": 1195.91, "end": 1196.53, "word": " العينة", "probability": 0.9239908854166666}, {"start": 1196.53, "end": 1196.63, "word": " في", "probability": 0.89697265625}, {"start": 1196.63, "end": 1197.15, "word": " بروتين", "probability": 0.801513671875}, {"start": 1197.15, "end": 1197.55, "word": " ولكن", "probability": 0.94287109375}, {"start": 1197.55, "end": 1197.91, "word": " أقل", "probability": 0.94921875}, {"start": 1197.91, "end": 1198.09, "word": " من", "probability": 0.99609375}, {"start": 1198.09, "end": 1198.43, "word": " واحد", "probability": 0.954833984375}, {"start": 1198.43, "end": 1198.71, "word": " ملي", "probability": 0.7900390625}, {"start": 1198.71, "end": 1199.07, "word": " جرام", "probability": 0.8600260416666666}, {"start": 1199.07, "end": 1199.45, "word": " لكل", "probability": 0.981201171875}, {"start": 1199.45, "end": 1199.77, "word": " ميل", "probability": 0.561767578125}, {"start": 1199.77, "end": 1200.79, "word": " بتقدرش", "probability": 0.9288330078125}, {"start": 1200.79, "end": 1200.97, "word": " هذه", "probability": 0.5166015625}, {"start": 1200.97, "end": 1201.35, "word": " الطريقة", "probability": 0.98583984375}, {"start": 1201.35, "end": 1201.95, "word": " تجيسه", "probability": 0.9166015625}, {"start": 1201.95, "end": 1203.27, "word": " بتعطيني", "probability": 0.807275390625}, {"start": 1203.27, "end": 1203.95, "word": " كميات", "probability": 0.995361328125}, {"start": 1203.95, "end": 1204.45, "word": " خاطر", "probability": 0.7831217447916666}], "temperature": 1.0}, {"id": 51, "seek": 123477, "start": 1205.55, "end": 1234.77, "text": "إذاً هذه الطريقة بتحتاج لكميات كبيرة من البروتين عشان تقدر تجيسها لو جبت عينة فيها كمية بروتين من واحد لعشرين ميلي جرام لكل ملت بتقدر تجيسها لو فيها أقل من واحد، بتقدرش تجيسها إذا العينة فيها بروتين ولكن أقل من واحد ميلي جرام برمال، بتقدرش تجيسها مش هتعطيكي قرار وهذه بنسميها sensitivity sensitivity تعريفها أقل كمية قادر 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"probability": 0.390625}, {"start": 1584.91, "end": 1585.45, "word": " تركيز", "probability": 0.994873046875}, {"start": 1585.45, "end": 1586.65, "word": " مختلف،", "probability": 0.931396484375}, {"start": 1586.65, "end": 1586.99, "word": " مثلا", "probability": 0.94970703125}, {"start": 1586.99, "end": 1587.41, "word": " الأولى", "probability": 0.95166015625}, {"start": 1587.41, "end": 1587.61, "word": " بيكون", "probability": 0.689697265625}, {"start": 1587.61, "end": 1587.93, "word": " مكتوب", "probability": 0.97119140625}, {"start": 1587.93, "end": 1588.21, "word": " عليها", "probability": 0.97021484375}, {"start": 1588.21, "end": 1588.39, "word": " إنه", "probability": 0.5089111328125}, {"start": 1588.39, "end": 1588.53, "word": " هي", "probability": 0.62353515625}, {"start": 1588.53, "end": 1588.85, "word": " 5", "probability": 0.708984375}, {"start": 1588.85, "end": 1589.33, "word": " milligram", "probability": 0.6396484375}, {"start": 1589.33, "end": 1589.71, "word": " per", 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0.9461263020833334}, {"start": 1820.08, "end": 1820.2, "word": " اللي", "probability": 0.976318359375}, {"start": 1820.2, "end": 1820.32, "word": " هي", "probability": 0.9599609375}, {"start": 1820.32, "end": 1820.86, "word": " تركيزها", "probability": 0.97890625}, {"start": 1820.86, "end": 1821.16, "word": " عشر", "probability": 0.888427734375}, {"start": 1821.16, "end": 1822.02, "word": " بقرأ", "probability": 0.8060302734375}, {"start": 1822.02, "end": 1822.14, "word": " ال", "probability": 0.98974609375}, {"start": 1822.14, "end": 1822.64, "word": " absorption", "probability": 0.9794921875}, {"start": 1822.64, "end": 1823.04, "word": " على", "probability": 0.91455078125}, {"start": 1823.04, "end": 1823.36, "word": " فرض", "probability": 0.956787109375}, {"start": 1823.36, "end": 1823.72, "word": " point", "probability": 0.9619140625}, {"start": 1823.72, "end": 1824.12, "word": " واحد", "probability": 0.971923828125}, {"start": 1824.12, "end": 1824.56, "word": " خمسة", "probability": 0.9903564453125}, {"start": 1824.56, "end": 1824.86, "word": " أربع", "probability": 0.8458658854166666}], "temperature": 1.0}, {"id": 78, "seek": 183701, "start": 1825.57, "end": 1837.01, "text": "باخد 25 لتركيزها 25 وبقرألها الـ absorption على فرد point 2 3 5 بعدين باخد لتركيزها 50 وبقرأ أعلى فرد point 3 3", "tokens": [3555, 47283, 3215, 3552, 5296, 2655, 31747, 1829, 11622, 11296, 3552, 46599, 4587, 2288, 10721, 1211, 11296, 2423, 39184, 27557, 15844, 6156, 2288, 3215, 935, 568, 805, 1025, 39182, 9957, 4724, 47283, 3215, 5296, 2655, 31747, 1829, 11622, 11296, 2625, 46599, 4587, 2288, 10721, 5551, 3615, 23942, 6156, 2288, 3215, 935, 805, 805], "avg_logprob": -0.21614582947006933, "compression_ratio": 1.4201680672268908, "no_speech_prob": 9.5367431640625e-07, "words": [{"start": 1825.57, "end": 1826.05, "word": "باخد", "probability": 0.8802083333333334}, {"start": 1826.05, "end": 1826.73, "word": " 25", "probability": 0.7763671875}, {"start": 1826.73, "end": 1827.53, "word": " لتركيزها", "probability": 0.89892578125}, {"start": 1827.53, "end": 1828.21, "word": " 25", "probability": 0.7626953125}, {"start": 1828.21, "end": 1828.81, "word": " وبقرألها", "probability": 0.7266845703125}, {"start": 1828.81, "end": 1828.95, "word": " الـ", "probability": 0.599609375}, {"start": 1828.95, "end": 1829.35, "word": " absorption", "probability": 0.7353515625}, {"start": 1829.35, "end": 1830.09, "word": " على", "probability": 0.44482421875}, {"start": 1830.09, "end": 1830.39, "word": " فرد", "probability": 0.6484375}, {"start": 1830.39, "end": 1830.67, "word": " point", "probability": 0.82275390625}, {"start": 1830.67, "end": 1831.09, "word": " 2", "probability": 0.8388671875}, {"start": 1831.09, "end": 1831.47, "word": " 3", "probability": 0.525390625}, {"start": 1831.47, "end": 1831.93, "word": " 5", "probability": 0.9853515625}, {"start": 1831.93, "end": 1833.09, "word": " بعدين", "probability": 0.875244140625}, {"start": 1833.09, "end": 1833.51, "word": " باخد", "probability": 0.9713541666666666}, {"start": 1833.51, "end": 1834.17, "word": " لتركيزها", "probability": 0.94921875}, {"start": 1834.17, "end": 1834.63, "word": " 50", "probability": 0.8017578125}, {"start": 1834.63, "end": 1835.13, "word": " وبقرأ", "probability": 0.75872802734375}, {"start": 1835.13, "end": 1835.49, "word": " أعلى", "probability": 0.8191731770833334}, {"start": 1835.49, "end": 1835.83, "word": " فرد", "probability": 0.9705403645833334}, {"start": 1835.83, "end": 1836.23, "word": " point", "probability": 0.95947265625}, {"start": 1836.23, "end": 1836.73, "word": " 3", "probability": 0.9619140625}, {"start": 1836.73, "end": 1837.01, "word": " 3", "probability": 0.98583984375}], "temperature": 1.0}, {"id": 79, "seek": 186479, "start": 1837.65, "end": 1864.79, "text": "تمام؟ هالجهة أنا بيصير عندي معروف تركيز لهذه المادة وأخدتها قرأتها في جهاز Spectrophotometer فصار عندي معروف اللي هي absorption إذا عندي معروف تركيز، concentration و absorption بجي برسم 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"word": " قرأتها", "probability": 0.8611328125}, {"start": 1844.23, "end": 1844.35, "word": " في", "probability": 0.96533203125}, {"start": 1844.35, "end": 1844.69, "word": " جهاز", "probability": 0.9944661458333334}, {"start": 1844.69, "end": 1845.85, "word": " Spectrophotometer", "probability": 0.83489990234375}, {"start": 1845.85, "end": 1846.31, "word": " فصار", "probability": 0.8854166666666666}, {"start": 1846.31, "end": 1846.57, "word": " عندي", "probability": 0.945556640625}, {"start": 1846.57, "end": 1847.01, "word": " معروف", "probability": 0.9894205729166666}, {"start": 1847.01, "end": 1847.17, "word": " اللي", "probability": 0.86767578125}, {"start": 1847.17, "end": 1847.31, "word": " هي", "probability": 0.298583984375}, {"start": 1847.31, "end": 1847.93, "word": " absorption", "probability": 0.95263671875}, {"start": 1847.93, "end": 1848.75, "word": " إذا", "probability": 0.702392578125}, {"start": 1848.75, "end": 1849.05, "word": " عندي", "probability": 0.794189453125}, {"start": 1849.05, "end": 1849.41, "word": " معروف", "probability": 0.99169921875}, {"start": 1849.41, "end": 1850.21, "word": " تركيز،", "probability": 0.90556640625}, {"start": 1850.21, "end": 1850.89, "word": " concentration", "probability": 0.83056640625}, {"start": 1850.89, "end": 1851.13, "word": " و", "probability": 0.912109375}, {"start": 1851.13, "end": 1851.65, "word": " absorption", "probability": 0.7177734375}, {"start": 1851.65, "end": 1852.75, "word": " بجي", "probability": 0.73583984375}, {"start": 1852.75, "end": 1853.15, "word": " برسم", "probability": 0.8811848958333334}, {"start": 1853.15, "end": 1854.01, "word": " الـStandard", "probability": 0.7447265625}, {"start": 1854.01, "end": 1854.59, "word": " Curve", "probability": 0.79296875}, {"start": 1854.59, "end": 1855.83, "word": " الـStandard", "probability": 0.9306640625}, {"start": 1855.83, "end": 1856.27, "word": " Curve", "probability": 0.97265625}, {"start": 1856.27, "end": 1856.55, "word": " كيف", 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1978.55, "word": " وين", "probability": 0.925048828125}, {"start": 1978.55, "end": 1978.91, "word": " وين", "probability": 0.809814453125}, {"start": 1978.91, "end": 1979.35, "word": " وصلت", "probability": 0.9713541666666666}, {"start": 1979.35, "end": 1979.73, "word": " عنديا", "probability": 0.892822265625}, {"start": 1979.73, "end": 1980.43, "word": " تركيز", "probability": 0.9188232421875}, {"start": 1980.43, "end": 1981.41, "word": " وبكتب", "probability": 0.9132080078125}, {"start": 1981.41, "end": 1982.05, "word": " تركيز", "probability": 0.990234375}, {"start": 1982.05, "end": 1982.31, "word": " هذه", "probability": 0.7001953125}, {"start": 1982.31, "end": 1982.83, "word": " العينة", "probability": 0.7687174479166666}, {"start": 1982.83, "end": 1983.43, "word": " المجهولة", "probability": 0.98759765625}], "temperature": 1.0}, {"id": 85, "seek": 199400, "start": 1984.26, "end": 1994.0, "text": "أنا عارف إنه صعب عليكم تفهموه هنا لكن هنوضحه في المعمل ونورسمه إن شاء الله على الصجورة ونوضح كيف بينعمل الـ Standard K", "tokens": [10721, 8315, 6225, 9640, 5172, 36145, 3224, 20328, 3615, 3555, 25894, 24793, 6055, 5172, 16095, 2407, 3224, 34105, 44381, 8032, 1863, 2407, 11242, 5016, 3224, 8978, 9673, 25957, 1211, 4032, 1863, 13063, 38251, 3224, 36145, 13412, 16606, 21984, 15844, 31767, 7435, 13063, 3660, 4032, 1863, 2407, 11242, 5016, 9122, 33911, 4724, 1829, 1863, 25957, 1211, 2423, 39184, 21298, 591], "avg_logprob": -0.2225260486205419, "compression_ratio": 1.4137931034482758, "no_speech_prob": 6.121397018432617e-05, "words": [{"start": 1984.26, "end": 1984.9, "word": "أنا", "probability": 0.80126953125}, {"start": 1984.9, "end": 1985.18, "word": " عارف", "probability": 0.9169921875}, {"start": 1985.18, "end": 1985.4, "word": " إنه", "probability": 0.802978515625}, {"start": 1985.4, "end": 1985.68, "word": " صعب", "probability": 0.9944661458333334}, {"start": 1985.68, "end": 1986.0, "word": " عليكم", "probability": 0.813720703125}, {"start": 1986.0, "end": 1986.62, "word": " تفهموه", "probability": 0.89931640625}, {"start": 1986.62, "end": 1986.9, "word": " هنا", "probability": 0.313232421875}, {"start": 1986.9, "end": 1987.96, "word": " لكن", "probability": 0.5791015625}, {"start": 1987.96, "end": 1988.98, "word": " هنوضحه", "probability": 0.9295247395833334}, {"start": 1988.98, "end": 1989.08, "word": " في", "probability": 0.96533203125}, {"start": 1989.08, "end": 1989.54, "word": " المعمل", "probability": 0.9913736979166666}, {"start": 1989.54, "end": 1990.08, "word": " ونورسمه", "probability": 0.7609375}, {"start": 1990.08, "end": 1990.14, "word": " إن", "probability": 0.70458984375}, {"start": 1990.14, "end": 1990.32, "word": " شاء", "probability": 0.981689453125}, {"start": 1990.32, "end": 1990.46, "word": " الله", "probability": 0.96533203125}, {"start": 1990.46, "end": 1990.64, "word": " على", "probability": 0.94873046875}, {"start": 1990.64, "end": 1991.06, "word": " الصجورة", "probability": 0.7393798828125}, {"start": 1991.06, "end": 1991.6, "word": " ونوضح", "probability": 0.78408203125}, {"start": 1991.6, "end": 1991.88, "word": " كيف", "probability": 0.9658203125}, {"start": 1991.88, "end": 1992.84, "word": " بينعمل", "probability": 0.723876953125}, {"start": 1992.84, "end": 1993.22, "word": " الـ", "probability": 0.700927734375}, {"start": 1993.22, "end": 1993.7, "word": " Standard", "probability": 0.52978515625}, {"start": 1993.7, "end": 1994.0, "word": " K", "probability": 0.70556640625}], "temperature": 1.0}, {"id": 86, "seek": 201935, "start": 1996.01, "end": 2019.35, "text": "شايفين هنا الرسم اللي تحت اللي هو تقاطع سيني مع صعدي خط السيني بيمثل الـ concentration والخط الصعدي بيمثل الـ absorption يكون عندي مواد جياني معروف تركيزها كل واحدة إلاها concentration مختلف وabsorption على الخط الصعدي أنا أخدت هذه العينات وقرأتها في جهاز Spectrophotometer وطلع معايا absorption", "tokens": [8592, 995, 33911, 9957, 34105, 34892, 38251, 13672, 1829, 6055, 33753, 13672, 1829, 31439, 6055, 4587, 41193, 3615, 8608, 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الها point واحد نات بقى بحط نقطة النقطة اللي بعدها لكل عينة جداش تركيزها ماجداش ال absorption بحط النقطة بوصل خط مستقيم يمر بمعظم النقاط", "tokens": [3224, 2655, 3555, 4587, 7578, 1975, 8592, 1829, 4724, 2304, 12984, 1211, 1975, 12610, 8717, 47432, 3660, 50113, 15040, 15844, 6156, 2288, 28671, 16490, 2304, 3794, 3660, 9154, 31767, 9957, 1829, 2423, 9856, 4032, 9154, 31767, 18513, 2288, 23032, 1211, 3615, 2423, 27557, 2423, 11296, 935, 36764, 24401, 8717, 995, 2655, 4724, 4587, 7578, 4724, 5016, 9566, 8717, 47432, 3660, 28239, 47432, 3660, 13672, 1829, 45030, 3215, 11296, 5296, 28820, 6225, 9957, 3660, 10874, 3215, 33599, 6055, 31747, 1829, 11622, 11296, 19446, 7435, 3215, 33599, 2423, 27557, 4724, 5016, 9566, 28239, 47432, 3660, 4724, 2407, 36520, 16490, 9566, 3714, 14851, 4587, 32640, 7251, 29973, 4724, 2304, 3615, 19913, 2304, 28239, 4587, 41193], "avg_logprob": -0.27820795194237635, "compression_ratio": 1.8271028037383177, "no_speech_prob": 0.00012683868408203125, "words": 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بحط عليها reagent و بعدين باخدها على جهاز الـ spectrophotometer", "tokens": [41185, 18871, 16254, 6225, 1829, 8315, 3660, 39894, 30544, 9841, 6225, 1829, 8315, 3660, 50113, 15040, 3714, 47283, 3215, 11296, 9154, 3714, 16572, 11242, 4032, 41185, 11296, 4724, 32887, 2655, 9957, 4724, 16254, 5551, 3615, 28480, 9122, 2304, 10632, 29538, 2423, 26890, 35473, 9957, 4724, 5016, 9566, 25894, 11296, 9154, 2423, 39184, 26949, 317, 30767, 1829, 2423, 39184, 3832, 4724, 25957, 1211, 11296, 20449, 10943, 37977, 2423, 39184, 3832, 4724, 5016, 9566, 25894, 11296, 26949, 317, 4032, 39182, 9957, 4724, 47283, 3215, 11296, 15844, 10874, 3224, 31377, 2423, 39184, 6177, 11741, 310, 13606], "avg_logprob": -0.22307180216971864, "compression_ratio": 1.8080808080808082, "no_speech_prob": 2.491474151611328e-05, "words": [{"start": 2041.0, "end": 2041.46, "word": "في", "probability": 0.1903076171875}, {"start": 2041.46, "end": 2041.94, "word": " عندي", "probability": 0.75732421875}, {"start": 2041.94, "end": 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وعارفة إيه لها الـ absorption جدّش الإمتصاص طلع بوصل على الخط المستقيم وبسقط عمودة على الـ concentration وبشوف وين يطلع تركيزها عند أي رقم هيك بكون أنا عارفة تركيز هذه المادة وبرضه هنرجع نوضح في المعمل كيف المخطط نرسمه وكيف نعمل على إيجاد تركيز أي مادة مشهولة", "tokens": [7435, 3215, 11703, 8592, 39896, 2423, 39184, 27557, 4724, 30544, 41850, 12174, 2288, 10721, 11296, 15844, 25724, 3224, 31377, 4032, 3615, 9640, 5172, 3660, 11933, 1829, 3224, 5296, 11296, 2423, 39184, 27557, 10874, 3215, 11703, 8592, 33688, 2304, 2655, 9381, 33546, 23032, 1211, 3615, 4724, 2407, 36520, 15844, 33962, 9566, 9673, 14851, 4587, 32640, 46599, 3794, 47432, 6225, 2304, 23328, 3660, 15844, 2423, 39184, 9856, 46599, 8592, 38688, 4032, 9957, 7251, 9566, 1211, 3615, 6055, 31747, 1829, 11622, 11296, 43242, 36632, 12602, 4587, 2304, 39896, 4117, 4724, 30544, 41850, 6225, 9640, 5172, 3660, 6055, 31747, 1829, 11622, 29538, 9673, 18513, 3660, 4032, 26890, 11242, 3224, 8032, 1863, 47341, 3615, 8717, 2407, 11242, 5016, 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"seek": 254979, "start": 2524.95, "end": 2549.79, "text": "ومن الـ Standard حطيت 1 من 10 مل يعني 100 مايكرون يعني 400 مايكرون و100 مايكرون يعني الـ total كله قداش 500 مايكرون، إذا الـ V2 هي 500 مايكرون، إذا صرت بعرف C1 2 عبارة عن 2 ميلي جرام، V1 هي 100 مايكرون، C2 بتديها، V2 هي 500 مايكرون", "tokens": [20498, 1863, 2423, 39184, 21298, 11331, 9566, 36081, 502, 9154, 1266, 3714, 1211, 37495, 22653, 2319, 19446, 1829, 37983, 11536, 37495, 22653, 8423, 19446, 1829, 37983, 11536, 4032, 6879, 19446, 1829, 37983, 11536, 37495, 22653, 2423, 39184, 3217, 28242, 3224, 12174, 3215, 33599, 5923, 19446, 1829, 37983, 11536, 12399, 11933, 15730, 2423, 39184, 691, 17, 39896, 5923, 19446, 1829, 37983, 11536, 12399, 11933, 15730, 20328, 43500, 45030, 28480, 383, 16, 568, 6225, 3555, 9640, 3660, 18871, 568, 3714, 1829, 20292, 10874, 2288, 10943, 12399, 691, 16, 39896, 2319, 19446, 1829, 37983, 11536, 12399, 383, 17, 39894, 16254, 11296, 12399, 691, 17, 39896, 5923, 19446, 1829, 37983, 11536], 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هي", "probability": 0.8662109375}, {"start": 2548.43, "end": 2548.99, "word": " 500", "probability": 0.98779296875}, {"start": 2548.99, "end": 2549.79, "word": " مايكرون", "probability": 0.99072265625}], "temperature": 1.0}, {"id": 109, "seek": 257373, "start": 2550.33, "end": 2573.73, "text": "من المعادلة 2 ضرب 100 على 500 بتطلع التركيز 4 من 10 فهمنا كيف جيبنا الـ Concentration لـ Tube 2 من القانون C1 V1 سواء C2 V2 منهي C1 هو تركيز الـ Standard اللي بدون تخفيف الأصل اللي جانب الشركة جدش كان 2 milligram per minute", "tokens": [27842, 9673, 3615, 18513, 37977, 568, 48812, 25513, 2319, 15844, 5923, 39894, 9566, 1211, 3615, 16712, 31747, 1829, 11622, 1017, 9154, 1266, 6156, 16095, 8315, 9122, 33911, 10874, 1829, 3555, 8315, 2423, 39184, 2656, 2207, 2405, 5296, 39184, 39313, 568, 9154, 25062, 7649, 11536, 383, 16, 691, 16, 8608, 2407, 16606, 383, 17, 691, 17, 9154, 3224, 1829, 383, 16, 31439, 6055, 31747, 1829, 11622, 2423, 39184, 21298, 13672, 1829, 47525, 11536, 6055, 9778, 5172, 33911, 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ل3", "probability": 0.729736328125}], "temperature": 1.0}, {"id": 112, "seek": 264371, "start": 2621.93, "end": 2643.71, "text": "ولا أربعة ولا خمسة وتعطوني الإجابة تسلموني الواجب إنه تحسبولي لتيوب رقم تلاتة واربعة وخمسة تيوب رقم تلاتة بيحكي إننا نحط عليها تلاتة من عشرة distilled water يعني 300 مايكرون واتنين من عشرة من البروتين ال standard يعني 200 مايكرون", "tokens": [12610, 995, 5551, 25513, 27884, 49429, 16490, 2304, 3794, 3660, 34683, 3615, 9566, 11536, 1829, 33688, 7435, 16758, 3660, 6055, 3794, 19528, 11536, 1829, 2423, 2407, 26108, 3555, 36145, 3224, 6055, 5016, 35457, 12610, 1829, 5296, 31371, 37746, 12602, 4587, 2304, 6055, 1211, 9307, 3660, 4032, 9640, 3555, 27884, 4032, 9778, 2304, 3794, 3660, 6055, 1829, 37746, 12602, 4587, 2304, 6055, 1211, 9307, 3660, 4724, 1829, 5016, 4117, 1829, 36145, 8315, 8717, 5016, 9566, 25894, 11296, 6055, 1211, 9307, 3660, 9154, 6225, 8592, 25720, 1483, 6261, 1281, 37495, 22653, 6641, 19446, 1829, 37983, 11536, 4032, 9307, 1863, 9957, 9154, 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0.8594563802083334}, {"start": 2879.8, "end": 2880.1, "word": " لون", "probability": 0.930908203125}, {"start": 2880.1, "end": 2880.26, "word": " الـ", "probability": 0.994384765625}, {"start": 2880.26, "end": 2880.66, "word": " Reagent", "probability": 0.8904622395833334}, {"start": 2880.66, "end": 2881.26, "word": " العصني", "probability": 0.7350260416666666}, {"start": 2881.26, "end": 2882.54, "word": " وهيقرأ", "probability": 0.8724772135416666}, {"start": 2882.54, "end": 2883.16, "word": " لون", "probability": 0.9794921875}, {"start": 2883.16, "end": 2883.84, "word": " التفاعل", "probability": 0.9801025390625}, {"start": 2883.84, "end": 2884.62, "word": " فأنا", "probability": 0.8382161458333334}, {"start": 2884.62, "end": 2885.44, "word": " بحط", "probability": 0.9562174479166666}, {"start": 2885.44, "end": 2885.92, "word": " الـ", "probability": 0.6513671875}, {"start": 2885.92, "end": 2886.18, "word": " Tube", "probability": 0.6044921875}, {"start": 2886.18, "end": 2886.7, "word": " بس", "probability": 0.964111328125}, {"start": 2886.7, "end": 2887.26, "word": " تحتوي", "probability": 0.8025716145833334}, {"start": 2887.26, "end": 2887.46, "word": " على", "probability": 0.89208984375}, {"start": 2887.46, "end": 2887.62, "word": " الـ", "probability": 0.987548828125}, {"start": 2887.62, "end": 2888.1, "word": " Reagent", "probability": 0.9529622395833334}, {"start": 2888.1, "end": 2888.72, "word": " عشان", "probability": 0.9752604166666666}, {"start": 2888.72, "end": 2889.34, "word": " أصفر", "probability": 0.924560546875}, {"start": 2889.34, "end": 2889.72, "word": " عليها", "probability": 0.964111328125}, {"start": 2889.72, "end": 2890.4, "word": " الجهاز", "probability": 0.99072265625}], "temperature": 1.0}, {"id": 124, "seek": 291530, "start": 2890.8, "end": 2915.3, "text": "إن الجهاز يقرأ كمية هذا اللون و بعد هي يخصومها كأنه بده يخصومها من التيوبات التانية زي إذا خدتوا في المقدمة كيف نوزن على الميزان كيف بنستخدم الميزان بحط ال cup مثلا اللي بدي أوزن فيه بدي أحط فيه المادة أول أجي بحط ال cup عشان إيه الوزن بصفر عليه و بعدين بحط المادة عشان يشيل وزنة ال cup", "tokens": [28814, 1863, 25724, 3224, 31377, 7251, 4587, 2288, 10721, 9122, 2304, 10632, 23758, 13672, 11536, 4032, 39182, 39896, 7251, 9778, 9381, 20498, 11296, 9122, 33456, 3224, 47525, 3224, 7251, 9778, 9381, 20498, 11296, 9154, 16712, 1829, 37746, 9307, 16712, 7649, 10632, 30767, 1829, 11933, 15730, 16490, 3215, 2655, 14407, 8978, 9673, 28543, 46007, 9122, 33911, 8717, 2407, 11622, 1863, 15844, 9673, 1829, 11622, 7649, 9122, 33911, 44945, 14851, 9778, 40448, 9673, 1829, 11622, 7649, 4724, 5016, 9566, 2423, 4414, 50113, 15040, 13672, 1829, 4724, 16254, 34051, 11622, 1863, 8978, 3224, 4724, 16254, 5551, 5016, 9566, 8978, 3224, 9673, 18513, 3660, 5551, 12610, 5551, 7435, 1829, 4724, 5016, 9566, 2423, 4414, 6225, 8592, 7649, 11933, 1829, 3224, 2423, 2407, 11622, 1863, 4724, 9381, 5172, 2288, 47356, 4032, 39182, 9957, 4724, 5016, 9566, 9673, 18513, 3660, 6225, 8592, 7649, 7251, 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{"start": 2894.82, "end": 2895.1, "word": " كأنه", "probability": 0.8727213541666666}, {"start": 2895.1, "end": 2895.32, "word": " بده", "probability": 0.474853515625}, {"start": 2895.32, "end": 2895.84, "word": " يخصومها", "probability": 0.98125}, {"start": 2895.84, "end": 2896.04, "word": " من", "probability": 0.97802734375}, {"start": 2896.04, "end": 2896.62, "word": " التيوبات", "probability": 0.78790283203125}, {"start": 2896.62, "end": 2897.3, "word": " التانية", "probability": 0.9747721354166666}, {"start": 2897.3, "end": 2897.98, "word": " زي", "probability": 0.904052734375}, {"start": 2897.98, "end": 2898.16, "word": " إذا", "probability": 0.96923828125}, {"start": 2898.16, "end": 2898.5, "word": " خدتوا", "probability": 0.7392578125}, {"start": 2898.5, "end": 2898.6, "word": " في", "probability": 0.95703125}, {"start": 2898.6, "end": 2899.18, "word": " المقدمة", "probability": 0.9798177083333334}, {"start": 2899.18, "end": 2899.44, "word": " كيف", "probability": 0.95849609375}, {"start": 2899.44, "end": 2899.94, "word": " نوزن", "probability": 0.9886474609375}, {"start": 2899.94, "end": 2900.12, "word": " على", "probability": 0.86572265625}, {"start": 2900.12, "end": 2900.68, "word": " الميزان", "probability": 0.988037109375}, {"start": 2900.68, "end": 2901.3, "word": " كيف", "probability": 0.825927734375}, {"start": 2901.3, "end": 2902.18, "word": " بنستخدم", "probability": 0.9622802734375}, {"start": 2902.18, "end": 2902.76, "word": " الميزان", "probability": 0.9923095703125}, {"start": 2902.76, "end": 2903.44, "word": " بحط", "probability": 0.8002115885416666}, {"start": 2903.44, "end": 2903.64, "word": " ال", "probability": 0.888671875}, {"start": 2903.64, "end": 2903.84, "word": " cup", "probability": 0.432373046875}, {"start": 2903.84, "end": 2904.16, "word": " مثلا", "probability": 0.969970703125}, {"start": 2904.16, "end": 2904.38, "word": " اللي", "probability": 0.926513671875}, {"start": 2904.38, "end": 2904.62, "word": " بدي", "probability": 0.863037109375}, {"start": 2904.62, "end": 2905.08, "word": " أوزن", "probability": 0.9187825520833334}, {"start": 2905.08, "end": 2905.54, "word": " فيه", "probability": 0.9951171875}, {"start": 2905.54, "end": 2905.9, "word": " بدي", "probability": 0.491455078125}, {"start": 2905.9, "end": 2906.12, "word": " أحط", "probability": 0.8958333333333334}, {"start": 2906.12, "end": 2906.28, "word": " فيه", "probability": 0.98828125}, {"start": 2906.28, "end": 2906.7, "word": " المادة", "probability": 0.9814453125}, {"start": 2906.7, "end": 2907.22, "word": " أول", "probability": 0.9111328125}, {"start": 2907.22, "end": 2907.38, "word": " أجي", "probability": 0.641845703125}, {"start": 2907.38, "end": 2907.74, "word": " بحط", "probability": 0.9000651041666666}, {"start": 2907.74, "end": 2907.88, "word": " ال", "probability": 0.85791015625}, {"start": 2907.88, "end": 2908.14, "word": " cup", "probability": 0.97509765625}, {"start": 2908.14, "end": 2908.48, "word": " عشان", "probability": 0.9205729166666666}, {"start": 2908.48, "end": 2908.66, "word": " إيه", "probability": 0.7239583333333334}, {"start": 2908.66, "end": 2909.14, "word": " الوزن", "probability": 0.9906005859375}, {"start": 2909.14, "end": 2910.24, "word": " بصفر", "probability": 0.833251953125}, {"start": 2910.24, "end": 2910.72, "word": " عليه", "probability": 0.9775390625}, {"start": 2910.72, "end": 2911.42, "word": " و", "probability": 0.6708984375}, {"start": 2911.42, "end": 2911.9, "word": " بعدين", "probability": 0.91162109375}, {"start": 2911.9, "end": 2912.34, "word": " بحط", "probability": 0.9944661458333334}, {"start": 2912.34, "end": 2912.88, "word": " المادة", "probability": 0.9912109375}, {"start": 2912.88, "end": 2913.4, "word": " عشان", "probability": 0.9879557291666666}, {"start": 2913.4, "end": 2914.02, "word": " يشيل", "probability": 0.9890950520833334}, {"start": 2914.02, "end": 2914.74, "word": " وزنة", "probability": 0.904296875}, {"start": 2914.74, "end": 2915.04, "word": " ال", "probability": 0.88134765625}, {"start": 2915.04, "end": 2915.3, "word": " cup", "probability": 0.97021484375}], "temperature": 1.0}, {"id": 125, "seek": 293526, "start": 2916.22, "end": 2935.26, "text": "وأنا بصفر على لون الـ region عشان بعد هيك أشيل لون الـ region عشان يعملني calibration فالمرة الجاي لما نشتغل هذه التجربة بدنا معانا ورق رسم بياني عشان نرسم عليه الـ standard curve", "tokens": [2407, 10721, 8315, 4724, 9381, 5172, 2288, 15844, 5296, 11536, 2423, 39184, 4458, 6225, 8592, 7649, 39182, 39896, 4117, 5551, 8592, 26895, 5296, 11536, 2423, 39184, 4458, 6225, 8592, 7649, 7251, 25957, 1211, 22653, 38732, 6156, 45340, 25720, 25724, 47302, 5296, 15042, 8717, 8592, 2655, 17082, 1211, 29538, 16712, 7435, 25513, 3660, 47525, 8315, 20449, 7649, 995, 4032, 2288, 4587, 12602, 38251, 4724, 1829, 7649, 1829, 6225, 8592, 7649, 8717, 2288, 38251, 47356, 2423, 39184, 3832, 7605], "avg_logprob": -0.151542471578488, "compression_ratio": 1.5846994535519126, "no_speech_prob": 3.5762786865234375e-07, "words": [{"start": 2916.22, "end": 2916.72, "word": "وأنا", "probability": 0.6558430989583334}, {"start": 2916.72, "end": 2917.78, "word": " بصفر", "probability": 0.9208984375}, {"start": 2917.78, "end": 2918.0, "word": " على", "probability": 0.90380859375}, {"start": 2918.0, "end": 2918.38, "word": " لون", "probability": 0.873291015625}, {"start": 2918.38, "end": 2918.58, "word": " الـ", "probability": 0.92626953125}, {"start": 2918.58, "end": 2919.02, "word": " region", "probability": 0.256591796875}, {"start": 2919.02, "end": 2920.0, "word": " عشان", "probability": 0.8914388020833334}, {"start": 2920.0, "end": 2920.36, "word": " بعد", "probability": 0.93310546875}, {"start": 2920.36, "end": 2920.8, "word": " هيك", "probability": 0.85302734375}, {"start": 2920.8, "end": 2921.34, "word": " أشيل", "probability": 0.784912109375}, {"start": 2921.34, "end": 2922.14, "word": " لون", "probability": 0.97509765625}, {"start": 2922.14, "end": 2922.64, "word": " الـ", "probability": 0.787841796875}, {"start": 2922.64, "end": 2922.96, "word": " region", "probability": 0.80419921875}, {"start": 2922.96, "end": 2923.26, "word": " عشان", "probability": 0.958984375}, {"start": 2923.26, "end": 2923.92, "word": " يعملني", "probability": 0.8712158203125}, {"start": 2923.92, "end": 2924.86, "word": " calibration", "probability": 0.88818359375}, {"start": 2924.86, "end": 2926.24, "word": " فالمرة", "probability": 0.9285481770833334}, {"start": 2926.24, "end": 2926.78, "word": " الجاي", "probability": 0.611572265625}, {"start": 2926.78, "end": 2927.48, "word": " لما", "probability": 0.832763671875}, {"start": 2927.48, "end": 2928.0, "word": " نشتغل", "probability": 0.9619140625}, {"start": 2928.0, "end": 2928.22, "word": " هذه", "probability": 0.74560546875}, {"start": 2928.22, "end": 2928.74, "word": " التجربة", "probability": 0.923828125}, {"start": 2928.74, "end": 2929.06, "word": " بدنا", "probability": 0.878173828125}, {"start": 2929.06, "end": 2929.46, "word": " معانا", "probability": 0.7779134114583334}, {"start": 2929.46, "end": 2929.98, "word": " ورق", "probability": 0.9869791666666666}, {"start": 2929.98, "end": 2930.62, "word": " رسم", "probability": 0.979248046875}, {"start": 2930.62, "end": 2931.5, "word": " بياني", "probability": 0.9656982421875}, {"start": 2931.5, "end": 2932.54, "word": " عشان", "probability": 0.9873046875}, {"start": 2932.54, "end": 2932.96, "word": " نرسم", "probability": 0.9915364583333334}, {"start": 2932.96, "end": 2933.36, "word": " عليه", "probability": 0.96875}, {"start": 2933.36, "end": 2933.82, "word": " الـ", "probability": 0.859619140625}, {"start": 2933.82, "end": 2934.18, "word": " standard", "probability": 0.63623046875}, {"start": 2934.18, "end": 2935.26, "word": " curve", "probability": 0.9326171875}], "temperature": 1.0}], "language": "ar", "language_probability": 1.0, "duration": 2937.835125, "duration_after_vad": 2872.936874999989}
\ No newline at end of file
diff --git a/PL9fwy3NUQKwZ1Q7zOg1ObgufPMjRI3WY1/tYZzyB0o5Ps_raw.srt b/PL9fwy3NUQKwZ1Q7zOg1ObgufPMjRI3WY1/tYZzyB0o5Ps_raw.srt
new file mode 100644
index 0000000000000000000000000000000000000000..3a2bcfe8b44a03c6ac54094fc579aa5b71c52383
--- /dev/null
+++ b/PL9fwy3NUQKwZ1Q7zOg1ObgufPMjRI3WY1/tYZzyB0o5Ps_raw.srt
@@ -0,0 +1,2588 @@
+1
+00:00:04,790 --> 00:00:08,130
+اليوم بدنا نحكي بموضوع الـ quantitative test for
+
+2
+00:00:08,130 --> 00:00:14,030
+protein هو فحوصات للكشف عن البروتين ولكن هذه
+
+3
+00:00:14,030 --> 00:00:19,030
+الفحوصات نوعها quantitative quantitative معناها كم
+
+4
+00:00:19,030 --> 00:00:23,730
+يعني الفحوصات كميةيعني بدي يطلع معانا في الآخر
+
+5
+00:00:23,730 --> 00:00:27,530
+تركيز للبروتين مش انه ظهور لون positive أو
+
+6
+00:00:27,530 --> 00:00:32,010
+negative زي ال qualitative انما quantitative يعني
+
+7
+00:00:32,010 --> 00:00:36,010
+بدي يظهر عندي تركيز للبروتين بدي يطلع عندي رقم
+
+8
+00:00:36,010 --> 00:00:43,650
+للبروتين جداش نسبة البروتين بدنا نحكي عن إيش اسمه
+
+9
+00:00:43,650 --> 00:00:48,630
+colorimetric assay colorimetric assay معناها تحليل
+
+10
+00:00:48,630 --> 00:00:54,260
+اللون فحوصات تحليل اللونلا كيف هذه الفحوصات هي
+
+11
+00:00:54,260 --> 00:00:59,340
+مجموعة من الفحوصات من خلالها بعرف تركيز أي مادة
+
+12
+00:00:59,340 --> 00:01:04,820
+بناء على التغير في اللون مجموعة من الفحوصات من
+
+13
+00:01:04,820 --> 00:01:10,520
+خلالها بعرف تركيز أي مادة بناء على التغير في اللون
+
+14
+00:01:10,520 --> 00:01:14,180
+ال law for indirect determination of specific
+
+15
+00:01:14,180 --> 00:01:20,000
+concentration تسمح بمعرفة تركيز المواد زي إيش هي
+
+16
+00:01:20,000 --> 00:01:24,500
+المواد الممكن تعرف تركيزهابروتين كربوهيدرات أو
+
+17
+00:01:24,500 --> 00:01:28,460
+انزين بناء على ياش color exchange بناء على التغير
+
+18
+00:01:28,460 --> 00:01:32,420
+باللون this reaction can be performed directly
+
+19
+00:01:32,420 --> 00:01:35,900
+inside the spectrophotometer طب وين هي لجيت أنا
+
+20
+00:01:35,900 --> 00:01:39,360
+بحط المواد و بتغير لونها؟ طب مين بدي يجيس كمية
+
+21
+00:01:39,360 --> 00:01:43,080
+التغير في هذا اللون و يطلعلي التركيز؟ بناء على
+
+22
+00:01:43,080 --> 00:01:48,420
+جهاز اسمه جهاز spectrophotometer جهاز
+
+23
+00:01:48,420 --> 00:01:56,330
+spectrophotometerوهو جهاز قياس الطيف الضوء جهاز
+
+24
+00:01:56,330 --> 00:02:03,710
+Spectrophotometer هو جهاز لقياس كمية الضوء التي
+
+25
+00:02:03,710 --> 00:02:10,290
+تنتصها العينة بناء على قانون Beer-Lambert Law هو
+
+26
+00:02:10,290 --> 00:02:16,070
+جهاز لقياس كمية الضوء التي تنتصها العينة بناء على
+
+27
+00:02:16,070 --> 00:02:21,520
+قانون Beer-Lambert Lawوقانون هذا إيش بيحكي؟ بيحكي
+
+28
+00:02:21,520 --> 00:02:26,440
+الـ intensity of color and absorption is directly
+
+29
+00:02:26,440 --> 00:02:30,720
+proportional to the concentration إنه كتافة لون
+
+30
+00:02:30,720 --> 00:02:34,900
+المادة اللون اللي أنا بحطه بحب بعد ما أخلط المادة
+
+31
+00:02:34,900 --> 00:02:38,800
+المراد قياسة على ال reaction اللون اللي بيظهر عندي
+
+32
+00:02:38,800 --> 00:02:43,700
+كتافة اللون هاي بتتناسب تناسب ترضي مع تركيز المادة
+
+33
+00:02:43,700 --> 00:02:48,110
+يعني كل ما زادت شدية اللونهيكون تركيز المادة
+
+34
+00:02:48,110 --> 00:02:56,790
+الموجود أعلى كل ما قلت شدة اللون هيقل
+
+35
+00:02:56,790 --> 00:03:02,290
+تركيز المادة علاقة ترضية بين كتافة لون المادة و
+
+36
+00:03:02,290 --> 00:03:04,790
+تركيز هذه المادة
+
+37
+00:03:07,450 --> 00:03:11,870
+إيش هي الطرق لتحديد تركيز البروتين؟ method for
+
+38
+00:03:11,870 --> 00:03:16,010
+protein determination طرق تحديد تركيز البروتين كيف
+
+39
+00:03:16,010 --> 00:03:19,750
+أنا بدي عندي عينة و بدي أعرف تركيز البروتين فيها؟
+
+40
+00:03:19,750 --> 00:03:22,890
+في أن عدة طرق في أن أحد الطرق هي الـ
+
+41
+00:03:22,890 --> 00:03:27,630
+spectrophotometry based on UV on ultraviolet light
+
+42
+00:03:27,630 --> 00:03:31,110
+اللي هو طريقة الـ spectrophotometry جهاز الـ
+
+43
+00:03:31,110 --> 00:03:35,830
+spectrophotometerبناء على مشاعر فوضوى نفسجية
+
+44
+00:03:35,830 --> 00:03:43,090
+Ultraviolet-Law هي طريقة تقليدية وقديمة البروتين
+
+45
+00:03:43,090 --> 00:03:48,190
+اكسيرت Ultraviolet-Light في 280 نانومتربتم قياسة
+
+46
+00:03:48,190 --> 00:03:54,530
+الضوء الممتاز عند ضوء طول موجي 280 نانو متر لكن
+
+47
+00:03:54,530 --> 00:03:58,010
+اكتشفوا ان الـ ultraviolet light هذي بتأثر على صحة
+
+48
+00:03:58,010 --> 00:04:03,230
+الإنسان وانها شعة ضارة فلجأوا إلى وجود طريقة أخرى
+
+49
+00:04:03,230 --> 00:04:06,710
+اللي هي الطريقة التانية الـ dye binding assay أو
+
+50
+00:04:06,710 --> 00:04:12,570
+اسمها barford assay dye binding assay أو barford
+
+51
+00:04:12,570 --> 00:04:17,820
+assayتعتمد على وجود صبغة ترتبط مع البروتين زي ما
+
+52
+00:04:17,820 --> 00:04:21,420
+وجود البروتين في العينة تيجي هذه الصبغة ترتبط مع
+
+53
+00:04:21,420 --> 00:04:26,520
+البروتين و بتعطيني لون و بجيس هذا اللون دي بند on
+
+54
+00:04:26,520 --> 00:04:30,500
+dye which bind to protein and give color هذه طريقة
+
+55
+00:04:30,500 --> 00:04:34,880
+اسمها dye binding assay أو parford assay و بعدين
+
+56
+00:04:34,880 --> 00:04:39,840
+اكتوروها لطريقة اخرى هي ال cover based assayالـ
+
+57
+00:04:39,840 --> 00:04:43,800
+cover-based assay هي فحوصات تعتمد على وجود الـ
+
+58
+00:04:43,800 --> 00:04:48,940
+cover بيجيسه كمية البروتين بناء على وجود الـ cover
+
+59
+00:04:48,940 --> 00:04:52,920
+وهي تلت طرق تعتمد على وجود الـ cover اللي هي أول
+
+60
+00:04:52,920 --> 00:04:58,280
+طريقة الـ Lawry-fullin method بيكنك كونك أسد أساي
+
+61
+00:04:58,280 --> 00:05:03,100
+Lawry-fullin method بيكنك كونك أسد أساي فيه هنا
+
+62
+00:05:03,100 --> 00:05:08,960
+الـ biuret methodوهدول الطرق اللي بتعتمد على وجود
+
+63
+00:05:08,960 --> 00:05:14,160
+الـ cover هي مستخدمة هي الـ routine use ال bio-red
+
+64
+00:05:14,160 --> 00:05:17,720
+هي الأكتر واحدة مستخدمة وهي اللي بيستخدموها هي
+
+65
+00:05:17,720 --> 00:05:22,360
+simple بسيطة وسهلة جمال وسريعة بتعتمد على أنه
+
+66
+00:05:22,360 --> 00:05:26,760
+يتكون لون هذا اللون بيعتمد على قانون Beer's law
+
+67
+00:05:26,760 --> 00:05:31,860
+أنه تتناسب تناسل ترضي شدت اللون مع تركيز المادة كل
+
+68
+00:05:31,860 --> 00:05:36,960
+ما زاد شدت اللون بزيد تركيز المادةكل هذه الطرق
+
+69
+00:05:36,960 --> 00:05:39,820
+اللي هي الـ copper based assay بتحتمد all this
+
+70
+00:05:39,820 --> 00:05:43,320
+method depends on the presence of cupric ion يعني
+
+71
+00:05:43,320 --> 00:05:46,800
+سواء الـ Lawry-fullon method الـ bicniconic acid
+
+72
+00:05:46,800 --> 00:05:51,380
+والبيروت method هدول التلات الطرق بتحتمد على وجود
+
+73
+00:05:51,380 --> 00:05:56,280
+ال cupric ion أن هو ريج إيري أيجاند من مكوناته ال
+
+74
+00:05:56,280 --> 00:06:01,130
+cupric ionحكينا قبل هيك إنه الـ Capric الـ Capar
+
+75
+00:06:01,130 --> 00:06:07,410
+بيعمل Cachelating Agent عامل متمخلب بيقدر يعمل عدة
+
+76
+00:06:07,410 --> 00:06:11,950
+روابط فبيجي على الروابط الببتيدية الموجودة في
+
+77
+00:06:11,950 --> 00:06:17,590
+البروتين وبيربط فيها من جميع الجهات تمام؟ فهذا الـ
+
+78
+00:06:17,590 --> 00:06:21,990
+Capric Ion بيكون موجود في الـ Reagent بيجي بيعمل
+
+79
+00:06:21,990 --> 00:06:25,050
+Cachelate Agent بيرتبط مع الـ peptide bond
+
+80
+00:06:25,050 --> 00:06:29,980
+وبيعطيني لهبحكي ليه إنه الـ Capric Ion اللي بيكون
+
+81
+00:06:29,980 --> 00:06:33,140
+موجود في الـ Reaction بيكون على شكل Capric Ion
+
+82
+00:06:33,140 --> 00:06:39,460
+اللي هي Cu Plus 2 بمجرد ما يرتبط في البروتين إذا
+
+83
+00:06:39,460 --> 00:06:43,440
+موجود البروتين في العينة أجل Capric Ion هذا ارتبط
+
+84
+00:06:43,440 --> 00:06:46,840
+مع البروتين وربط الروابط اللي بيبتديها مع بعضها
+
+85
+00:06:46,840 --> 00:06:52,320
+البعض بيصير له reduction إلى Capric Ionالـ Capric
+
+86
+00:06:52,320 --> 00:06:55,420
+Ion اللي موجود في الـ reagent بيكون موجود على شكل
+
+87
+00:06:55,420 --> 00:07:00,020
+Capric Ion Cu Plus II مجرد ما يرتبط مع البروتين
+
+88
+00:07:00,020 --> 00:07:03,960
+بيصير له reduction اختزال إلى Capric Ion Cu Plus
+
+89
+00:07:03,960 --> 00:07:09,760
+ففي عندنا تلت طرق لتحديد تركيز البروتين أول طريقة
+
+90
+00:07:09,760 --> 00:07:13,580
+الـ spectrophotometry وتاني طريقة اللي هي الـ dye
+
+91
+00:07:13,580 --> 00:07:17,960
+binding assay أو Barford Assay والتالتة الـ copper
+
+92
+00:07:17,960 --> 00:07:19,540
+based assay
+
+93
+00:07:23,090 --> 00:07:26,190
+هيش الـ aim and significance اللي هي الهدف
+
+94
+00:07:26,190 --> 00:07:31,930
+والأهمية لما عملنا اليوم أول شي الأهداف الـ aim to
+
+95
+00:07:31,930 --> 00:07:36,550
+perform bioretest for البروتين لقدرة على إجراء فحص
+
+96
+00:07:36,550 --> 00:07:40,790
+ال bio to estimate the amount of total proteins in
+
+97
+00:07:40,790 --> 00:07:45,470
+sample قدرة على تقدير كمية البروتين الموجود في
+
+98
+00:07:45,470 --> 00:07:50,990
+العينة وآخر شي to make standard كافي لقدرة على رسم
+
+99
+00:07:50,990 --> 00:07:55,360
+المنحنة المعياريالـ Standard K اللي هنحكي عنه
+
+100
+00:07:55,360 --> 00:07:59,860
+لقدام طيب الـ significance اللي همية أنا ليش بدي
+
+101
+00:07:59,860 --> 00:08:03,800
+أقدر تركيز البروتين أهمية هذا المعمل أني أعرف
+
+102
+00:08:03,800 --> 00:08:08,080
+quantization of البروتين لأن هذا is important and
+
+103
+00:08:08,080 --> 00:08:10,960
+has many applications in clinical laboratory
+
+104
+00:08:10,960 --> 00:08:15,640
+practice لتقدير الأهمية من معملنا أني أقدر تركيز
+
+105
+00:08:15,640 --> 00:08:19,920
+البروتينلأن كمية هذا البروتين أني أعرفها إلها
+
+106
+00:08:19,920 --> 00:08:25,060
+أهميات وإلها ضرورة تساعدني في تقييم حالة المريض،
+
+107
+00:08:25,060 --> 00:08:28,020
+هل في نقص في البروتين معين، يعني الخلل من عند هذا
+
+108
+00:08:28,020 --> 00:08:31,780
+البروتين في شركات معينة مثلا، لما في عندها مواد
+
+109
+00:08:31,780 --> 00:08:35,740
+تعرف كم تركيز البروتين فيها أو نسبة البروتين في
+
+110
+00:08:35,740 --> 00:08:39,260
+الجسم، بدي أعرف كم نسبة البروتين عند هذا الشخص
+
+111
+00:08:39,260 --> 00:08:44,760
+فإني أقدر كمية البروتين و أعرف كمية البروتين، هذا
+
+112
+00:08:44,760 --> 00:08:51,990
+إيش مهمفهي الأهمية من معملنا هنا بنحكي عن الـ
+
+113
+00:08:51,990 --> 00:08:56,630
+Bioret reagent إذا فاكرين في معمل qualitative test
+
+114
+00:08:56,630 --> 00:09:00,570
+of amino acid كان من ضمن الفحوصات فحص الـ Bioret
+
+115
+00:09:00,570 --> 00:09:05,370
+test الـ Bioret test كنا بنحط المادة على Bioret
+
+116
+00:09:05,370 --> 00:09:10,500
+reagent إذا اتغير اللون لأ الviolet إذا هذاإن هو
+
+117
+00:09:10,500 --> 00:09:13,480
+positive إنه البروتين موجود إنه فيه peptide bond
+
+118
+00:09:13,480 --> 00:09:16,860
+كنا بس نشوف هل اتغير اللون ولا لأ إذا اتغير
+
+119
+00:09:16,860 --> 00:09:20,160
+positive إذا ما اتغيرش negative هنا نفس الأشياء
+
+120
+00:09:20,160 --> 00:09:24,260
+إننا نشتغل على فحص بيور ال test ولكن بعد تغير
+
+121
+00:09:24,260 --> 00:09:27,600
+اللون بدلاش احنا عارفين إنه فيه بروتين بس هذا
+
+122
+00:09:27,600 --> 00:09:30,800
+اللون بدنا ناخده نجيسه في جهاز ال
+
+123
+00:09:30,800 --> 00:09:35,570
+spectrophotometerانجيزه على طول موجي معين عشان
+
+124
+00:09:35,570 --> 00:09:40,270
+يطلعلي رقم يطلعلي اللي هو كمية الضوء اللي انتصها
+
+125
+00:09:40,270 --> 00:09:45,190
+العينة بيطلعلي رقم بيطلعلي absorption انتصاص جديش
+
+126
+00:09:45,190 --> 00:09:49,310
+كمية الانتصاص عشان اعرف تركيز العينة انا في الآخر
+
+127
+00:09:49,310 --> 00:09:54,790
+بدي اطلع بتركيز للعينة مش بس تغير في لون positive
+
+128
+00:09:54,790 --> 00:09:58,100
+ولا negativeلأن حكينا بالأول إن اليوم فحصنا
+
+129
+00:09:58,100 --> 00:10:02,180
+quantitative test of a protein طب مين من الفحوصات
+
+130
+00:10:02,180 --> 00:10:05,100
+اللي أخدناها في أول معمل تكشف عن وجود ال protein
+
+131
+00:10:05,100 --> 00:10:09,560
+هو فحص ال bio rate test أخدناها في أول معمل إنه
+
+132
+00:10:09,560 --> 00:10:12,460
+بيكشف على وجود الرابط ال BPD الموجودة في ال
+
+133
+00:10:12,460 --> 00:10:16,120
+protein اليوم إننا نشتغل هذا ال bio rate ولكن بعد
+
+134
+00:10:16,120 --> 00:10:21,300
+ما نشتغله ناخده ونجيس كمية اللون أو تركيز المادة
+
+135
+00:10:21,300 --> 00:10:25,340
+بناء على التغير في اللون في جهاز spectrophotometer
+
+136
+00:10:26,480 --> 00:10:29,900
+إذا فاكرين حكينا إن الـ Bioretary agent بيتكون من
+
+137
+00:10:29,900 --> 00:10:34,720
+أدة مكونات حكينا إن هو عبارة عن قبر صلفات في وجود
+
+138
+00:10:34,720 --> 00:10:41,500
+alkaline media اللي هي NaOH أو KOHوفي مكون تالت ما
+
+139
+00:10:41,500 --> 00:10:45,120
+حكينا عنه اللي هو بوتاسيوم صوديوم ترتريت لذا الـ
+
+140
+00:10:45,120 --> 00:10:48,560
+bioreactient بيتكون من تلت مكونات اللي هم
+
+141
+00:10:48,560 --> 00:10:52,220
+الكبرسالفيت بوتاسيوم هيدروكسايد أو صوديوم
+
+142
+00:10:52,220 --> 00:10:56,880
+هيدروكسايد و بوتاسيوم صوديوم ترتريت بيجي الأول
+
+143
+00:10:56,880 --> 00:11:02,000
+مكون اللي هو الكبرسالفيت الكبرسالفيت هذا it's this
+
+144
+00:11:02,000 --> 00:11:06,300
+provides a capric ion which form chelate complex
+
+145
+00:11:06,930 --> 00:11:09,530
+والـ Capric Ion gives the reagent its
+
+146
+00:11:09,530 --> 00:11:13,730
+characteristic blue color إذا الكبرسالفيات وظيفته
+
+147
+00:11:13,730 --> 00:11:16,630
+ليش موجود في ال reagent عشان يعطيهم ال Capric Ion
+
+148
+00:11:16,630 --> 00:11:21,810
+بيزوّتني بال Capric Ion اللي حكينا إنه هذا أهم
+
+149
+00:11:21,810 --> 00:11:26,650
+عامل لازم يكون موجود عشان يشتغل ككلّات agent اللي
+
+150
+00:11:26,650 --> 00:11:30,730
+هو عامل متمخلب يربط الروابط اللي بيبتديها مع بعضها
+
+151
+00:11:30,730 --> 00:11:36,770
+لبعض تكوين كلّات complexإذا فاكرين لما شوفتوا لون
+
+152
+00:11:36,770 --> 00:11:40,650
+الـ bioretary agent لونه أزرق مين أعطاه هذا اللون
+
+153
+00:11:40,650 --> 00:11:44,010
+هو الـ capric ion هو المسؤول عن اللون الأزرق
+
+154
+00:11:44,010 --> 00:11:49,270
+الموجود في الـ reaction المكون التاني بوتاسيوم
+
+155
+00:11:49,270 --> 00:11:52,970
+هيدروكسيد أو صوديوم هيدروكسيد هذا ما بيساهم
+
+156
+00:11:52,970 --> 00:11:57,150
+بالتفاعل doesn't participate in the reaction but
+
+157
+00:11:57,150 --> 00:12:01,530
+provides the alkaline medium وإنما بيرفع البيئة مش
+
+158
+00:12:01,530 --> 00:12:06,560
+بس عشان يعطيني alkalineبيئة في الإعطاء وسط قاعدة
+
+159
+00:12:06,560 --> 00:12:10,780
+Potassium Sodium ترتريت المكون التالت اللي قد
+
+160
+00:12:10,780 --> 00:12:14,920
+تتواظهان أول وظيفة Stabilize the chelate complex
+
+161
+00:12:14,920 --> 00:12:19,140
+بيحافظ على ثباتية ال complex بعد ما يتكون ال
+
+162
+00:12:19,140 --> 00:12:22,640
+chelate complex ييجي الكبر يرتبط مع الرواد
+
+163
+00:12:22,640 --> 00:12:26,420
+البروتينية الموجودة في البروتين ويكون complex اسم
+
+164
+00:12:26,420 --> 00:12:30,560
+ال chelate اللي هو هذا بيحول اللور من أزرق إلى
+
+165
+00:12:30,560 --> 00:12:34,800
+violetإذا positive اتحول كان لـviolet من blue to
+
+166
+00:12:34,800 --> 00:12:38,900
+violet فالميلي اللي خلّاه يتحول الهول كابر أجر طبط
+
+167
+00:12:38,900 --> 00:12:43,360
+مع الروابط البوكتيرية وكوان الكليات كامة الـ
+
+168
+00:12:43,360 --> 00:12:46,680
+Potassium Sodium Tertarate بيحافظ على ثباتية هذا
+
+169
+00:12:46,680 --> 00:12:49,300
+ال complex إنه بعد ما يتكون هذا ال complex
+
+170
+00:12:49,300 --> 00:12:54,020
+مايتفككش، مايتكسرش تاني، يظل متكون، يظل ثابت عشان
+
+171
+00:12:54,020 --> 00:12:58,140
+أظن اللون موجود أحافظ على اللون و أروح أجهز تركيز
+
+172
+00:12:58,810 --> 00:13:02,050
+تاني وظيفة prevent precipitation of copper
+
+173
+00:13:02,050 --> 00:13:07,950
+hydroxide بيمنع ترصب الكبر لأنه لو ترصبها دو نزل
+
+174
+00:13:07,950 --> 00:13:11,810
+في القائز مش هيقدر يجي يرتبط مع الروابط الببتدية
+
+175
+00:13:11,810 --> 00:13:16,230
+ولا يقدر يعمل الكلات complex فهو بيحافظ على أنه
+
+176
+00:13:16,230 --> 00:13:20,890
+يمنع ترصبالـ cover بوتاسيوم بـ prevent auto
+
+177
+00:13:20,890 --> 00:13:25,790
+reduction of cover البوتاسيوم الموجود بهذه المكوّن
+
+178
+00:13:25,790 --> 00:13:30,150
+بيمنع الاختزال الذاتي للـ cover انه يصير ان الـ
+
+179
+00:13:30,150 --> 00:13:33,570
+Capric Ion اللي موجود في الـ reagent يختزل بدون ما
+
+180
+00:13:33,570 --> 00:13:36,830
+يرتبط في البروتين يصير الاختزال الذاتي ويتحول إلى
+
+181
+00:13:36,830 --> 00:13:40,690
+Capric Ion نحكي لبي مجرد ما الـ Capric Ion هذا
+
+182
+00:13:40,690 --> 00:13:44,530
+اللي موجود في الـ reagent يرتبط مع البروتين بيتحول
+
+183
+00:13:44,530 --> 00:13:50,250
+إلى Capric Ionوبيتغير اللون فمجرد ما يرتبط لازم
+
+184
+00:13:50,250 --> 00:13:55,440
+يصير اختزال ويتغير اللونطب ممكن يصير له auto
+
+185
+00:13:55,440 --> 00:13:58,860
+reduction اختزال ذاتي من نفسه يختزل حاله بدون ما
+
+186
+00:13:58,860 --> 00:14:02,900
+يرتبط يتحول من Capric Ion لCapric Ion ويتغير اللون
+
+187
+00:14:02,900 --> 00:14:06,880
+وممكن ما يكونش موجود البروتين لكنه اختزل ذاتيا
+
+188
+00:14:06,880 --> 00:14:10,840
+فعطاني false positive فعطاني نتيجة positive و
+
+189
+00:14:10,840 --> 00:14:15,020
+البروتين مش موجود فمين بده يمنح حدوث هذا الاختزال
+
+190
+00:14:15,020 --> 00:14:19,620
+الذاتي هو البوتاسيوم الموجود فيه مكون من مكونات
+
+191
+00:14:19,620 --> 00:14:23,150
+الـ reagentاللي هو الـ Potassium Sodium Tertiary
+
+192
+00:14:23,150 --> 00:14:30,710
+فعنا تلت وظائف للـ Potassium Sodium Tertiary إيش
+
+193
+00:14:30,710 --> 00:14:35,810
+ال principle اللي عملنا اليوم ال principle أنه أنا
+
+194
+00:14:35,810 --> 00:14:40,890
+بدي أجيس كمية ال protein determining total protein
+
+195
+00:14:40,890 --> 00:14:43,570
+in sample is the biorhythm method by biorhythm
+
+196
+00:14:43,570 --> 00:14:46,830
+method أن أنا بدي أجيس كمية البروتين الموجودة في
+
+197
+00:14:46,830 --> 00:14:51,620
+العينة بطريقة ال biorhythm methodالـ Biuret Method
+
+198
+00:14:51,620 --> 00:14:56,200
+حكي أنها بتعتمد على وجود الـ Capric Ion الـ Capric
+
+199
+00:14:56,200 --> 00:15:00,200
+بيجي بيعمل Complexation to the peptide bond in the
+
+200
+00:15:00,200 --> 00:15:03,080
+protein بيروح الـ Capric Ion اللي موجود في
+
+201
+00:15:03,080 --> 00:15:06,980
+الرياجان بيرتبط مع الـ peptide bond بيعمل كلات
+
+202
+00:15:06,980 --> 00:15:11,820
+Complex Formation of Capric Protein Complex اللي
+
+203
+00:15:11,820 --> 00:15:14,700
+هو الكبر مع البروتين بيعملوله Complex بيحتاج إلى
+
+204
+00:15:14,700 --> 00:15:19,100
+two peptide bondsحكينا إنه بحتاج إلى رابطتين
+
+205
+00:15:19,100 --> 00:15:23,240
+بتدياتين على الأقل عشان يعمل ال complex بيتحول
+
+206
+00:15:23,240 --> 00:15:27,290
+اللون إلى violet colorكلية البروداكت هذا اللون
+
+207
+00:15:27,290 --> 00:15:30,610
+اللي اتكون ال complex اللي اتكون ولونه violet
+
+208
+00:15:30,610 --> 00:15:35,370
+بجيثه measured by absorption spectroscopy at 540
+
+209
+00:15:35,370 --> 00:15:40,630
+nanometer بروح باجيث كمية امتصاص اللون الدوق اللي
+
+210
+00:15:40,630 --> 00:15:44,710
+امتصها العينة على جهاز spectrophotometer على طول
+
+211
+00:15:44,710 --> 00:15:50,740
+موجي 540 nanometerبحكي للـ molecule containing two
+
+212
+00:15:50,740 --> 00:15:53,620
+or more peptide bonds associated with the capric
+
+213
+00:15:53,620 --> 00:16:02,190
+ion to form a complexبيربول قرر تحوي على
+
+214
+00:16:02,190 --> 00:16:08,790
+رابطتين ببتديتين وأكتر بيرتبط مع الـ Capric Ion
+
+215
+00:16:08,790 --> 00:16:14,070
+وبيعمل لي كمبليكس كيلية كمبليكس لونه بيربول بجيسه
+
+216
+00:16:14,070 --> 00:16:19,690
+على خمسمية وأربعين نانو متر في وجود alkaline pH
+
+217
+00:16:19,690 --> 00:16:24,390
+حكينا لازم يكون أن alkaline pH الكبرك ايون الموجود
+
+218
+00:16:24,390 --> 00:16:29,970
+في ال regionبيرتبط مع الـ peptide bond بمجرد ما
+
+219
+00:16:29,970 --> 00:16:34,270
+يرتبط بيصير له reduction of من capric ion to
+
+220
+00:16:34,270 --> 00:16:38,930
+caprous ion يعني ملخص الـ principle لأن هو نفس الـ
+
+221
+00:16:38,930 --> 00:16:42,950
+principle تبع ال bio-test اللي أخدناه في معمل الـ
+
+222
+00:16:42,950 --> 00:16:46,690
+quality test of amino acid أخدنا أن الـ principle
+
+223
+00:16:46,690 --> 00:16:52,080
+حكيتبناه على شكل معادلة لأن الـ proteinزائد copper
+
+224
+00:16:52,080 --> 00:16:58,520
+sulfate في وجود قاعدة اللي هي NAH أو KOH كمان ضفنا
+
+225
+00:16:58,520 --> 00:17:01,880
+عليها اليوم Sodium Potassium Tertarate المكون
+
+226
+00:17:01,880 --> 00:17:07,620
+التالت بيعطينا كلية Complex Lonoviolet هذا الـ
+
+227
+00:17:07,620 --> 00:17:10,300
+принцип اللي أخدناه في أول معمل إيش بده أضيف عليه؟
+
+228
+00:17:10,300 --> 00:17:13,660
+إنه كلية Complex هذا اللي Lonoviolet ريد
+
+229
+00:17:13,660 --> 00:17:19,420
+absorption at 540 نانومتران انا مش بس بدي اشوف
+
+230
+00:17:19,420 --> 00:17:23,940
+اللون لأ بدي اقرأ الانتصاص لهذا اللون عند خمسمية
+
+231
+00:17:23,940 --> 00:17:28,920
+واربعين نانو متر لانه فحصنا اليوم quantitative بدي
+
+232
+00:17:28,920 --> 00:17:31,720
+اطلع عندي كمية مش qualitative
+
+233
+00:17:34,470 --> 00:17:37,890
+حكينا إنه في طرقتين تانيات كمان بيعتمدوا على الـ
+
+234
+00:17:37,890 --> 00:17:40,590
+CABAR-Based السائلي حكينا إنه إحنا عندنا الـ
+
+235
+00:17:40,590 --> 00:17:43,790
+Bioret في عندنا الـ Bicnic Conic Acid وفي عندنا
+
+236
+00:17:43,790 --> 00:17:48,090
+الـ Lowry Method إيش هي ال Lowry Method؟ ال Lowry
+
+237
+00:17:48,090 --> 00:17:52,590
+Method هو فحص بيعتمد على وجود الـ CABAR إنه فيه
+
+238
+00:17:52,590 --> 00:17:56,690
+Reagent اسمه الـ Falling Reagentنوكابرس ايون
+
+239
+00:17:56,690 --> 00:18:01,810
+detected with فلين كيوكالتيو فلين reagent هذا
+
+240
+00:18:01,810 --> 00:18:06,830
+الفلين reagent منه مكوّنين يتكون من حشتين من
+
+241
+00:18:06,830 --> 00:18:13,390
+فوسفوموليبدك و فوسفوتانجستك أسنإذا استخدمت فولين
+
+242
+00:18:13,390 --> 00:18:16,430
+رياجانت اللي بيتكون من فوسفوموليبديك أسد و
+
+243
+00:18:16,430 --> 00:18:20,150
+فوسفوتانجستيك أسن بنسمي هذه الطريقة الـ Lowry
+
+244
+00:18:20,150 --> 00:18:24,510
+method this method is commonly referred to as the
+
+245
+00:18:24,510 --> 00:18:28,470
+Lowry method كيف هي بتشتغل؟ بتعمل على تحويل زي
+
+246
+00:18:28,470 --> 00:18:33,880
+البييروت بتحول كابريك أيون إلى كابرس أيونبيعمل
+
+247
+00:18:33,880 --> 00:18:37,780
+كلات مع الرابط الـ PPD الموجودة في البروتين
+
+248
+00:18:37,780 --> 00:18:42,820
+بيعطيني لون blue Capric ion reduction of following
+
+249
+00:18:42,820 --> 00:18:46,740
+reagent produce a blue color الـ blue color هي
+
+250
+00:18:46,740 --> 00:18:49,640
+اللون الأزرق اللي بيظهر عندي بروح بقرأ له ال
+
+251
+00:18:49,640 --> 00:18:56,280
+absorption بس على طول موجي 650 إلى 750 nanometer
+
+252
+00:18:58,100 --> 00:19:02,160
+بحكيلي كمية اللون اللى زهرت عندى او شدة اللون
+
+253
+00:19:02,160 --> 00:19:06,280
+تعتمد على كمية الرابطة البيبتيدية الـ peptide bond
+
+254
+00:19:06,280 --> 00:19:09,980
+amount of peptide bond كل ما زادت الـ peptide bond
+
+255
+00:19:09,980 --> 00:19:15,520
+عدد رابط البيبتيدية اكيد هتزيد عندى شدة اللون يعني
+
+256
+00:19:15,520 --> 00:19:20,000
+هيزيد تركيز المادة لان كمية البروتين اللى موجوده
+
+257
+00:19:20,000 --> 00:19:25,820
+هيكون اعلىDisadvantage of biuret test في عندنا
+
+258
+00:19:25,820 --> 00:19:29,700
+عيوب لطريقة الـ biuret test الـ biuret test هي
+
+259
+00:19:29,700 --> 00:19:34,340
+طريقة لقياس البروتين في الهيوم أول مشكلة هي إنه
+
+260
+00:19:34,340 --> 00:19:38,560
+this method required large amount of protein for
+
+261
+00:19:38,560 --> 00:19:43,980
+detection إن هاي الطريقة تحتاج إلى كميات كبيرة من
+
+262
+00:19:43,980 --> 00:19:48,920
+البروتين عشان تقدر تكشفانه يعني هي بتجيس من واحد
+
+263
+00:19:48,920 --> 00:19:53,610
+لعشرين milligram per minuteبتجيس كميات البروتين
+
+264
+00:19:53,610 --> 00:19:57,910
+واحد لعشرين إذا كان في العينة في بروتين ولكن أقل
+
+265
+00:19:57,910 --> 00:20:01,950
+من واحد ملي جرام لكل ميل بتقدرش هذه الطريقة تجيسه
+
+266
+00:20:01,950 --> 00:20:07,870
+بتعطيني كميات خاطرإذاً هذه الطريقة بتحتاج لكميات
+
+267
+00:20:07,870 --> 00:20:11,370
+كبيرة من البروتين عشان تقدر تجيسها لو جبت عينة
+
+268
+00:20:11,370 --> 00:20:14,810
+فيها كمية بروتين من واحد لعشرين ميلي جرام لكل ملت
+
+269
+00:20:14,810 --> 00:20:19,050
+بتقدر تجيسها لو فيها أقل من واحد، بتقدرش تجيسها
+
+270
+00:20:19,050 --> 00:20:22,730
+إذا العينة فيها بروتين ولكن أقل من واحد ميلي جرام
+
+271
+00:20:22,730 --> 00:20:27,250
+برمال، بتقدرش تجيسها مش هتعطيكي قرار وهذه بنسميها
+
+272
+00:20:27,250 --> 00:20:33,270
+sensitivity sensitivity تعريفها أقل كمية قادر
+
+273
+00:20:33,270 --> 00:20:39,700
+الفحص على قياسهاأقل كمية بيقدر الفحص يجيسها بسميها
+
+274
+00:20:39,700 --> 00:20:43,560
+الـ sensitivity فلو سألت ال sensitivity للفحص ال
+
+275
+00:20:43,560 --> 00:20:49,000
+bio retest يداش هي الـ 1 milligram برميل أقل كمية
+
+276
+00:20:49,000 --> 00:20:52,080
+بيقدر ال bio retest يجيسها هي 1 milligram برميل
+
+277
+00:20:52,080 --> 00:20:55,740
+إذا ال sensitivity لل bio retest هي 1 milligram
+
+278
+00:20:55,740 --> 00:21:00,820
+برميل تعتبر ال sensitivity له قليلة أول عيوب هذه
+
+279
+00:21:00,820 --> 00:21:06,030
+الطريقة أن ال sensitivity له قليلةتاني من عيوبه ان
+
+280
+00:21:06,030 --> 00:21:09,530
+هي sensitive to a variety of nitrogen-containing
+
+281
+00:21:09,530 --> 00:21:15,590
+substances ان هذه طريقة حساسة لأي مركب كيميائي
+
+282
+00:21:15,590 --> 00:21:21,150
+يحتوي على رابطتين بيبتديتين فاكترالجيت هو بيتمد
+
+283
+00:21:21,150 --> 00:21:25,450
+على وجود رابطة بيبتدية تنتن في أكتر بيجي في الـ
+
+284
+00:21:25,450 --> 00:21:28,730
+Cupric Ion اللي موجود في الرياجان ويرتبط مع هذه
+
+285
+00:21:28,730 --> 00:21:33,430
+الرابطة البيبتدية وبيكوّن لكلات Complex اللي لون
+
+286
+00:21:33,430 --> 00:21:38,800
+violet وبجيسه على طول موجي 540 نانومترالرابطتين
+
+287
+00:21:38,800 --> 00:21:42,360
+البيبتيديتين هدول موجودين في البروتين بحط على
+
+288
+00:21:42,360 --> 00:21:46,980
+البروتين bio-retraction وبجيس كمية الكلية ال
+
+289
+00:21:46,980 --> 00:21:50,040
+complex اللي اتكون وعطاني اللون بجيس شدت هذا اللون
+
+290
+00:21:50,040 --> 00:21:55,080
+في الجهات ممكن في مركبات تانية تحتوي على رابطتين
+
+291
+00:21:55,080 --> 00:21:59,140
+بيبتيديتين فعلا زي مين؟ زي المركب اسمه الـ Bioria
+
+292
+00:21:59,140 --> 00:22:03,360
+بيوريا معناهت ان مركبين يوريا بيرتبطوا مع بعض
+
+293
+00:22:03,360 --> 00:22:07,930
+بيقدروا يرتبطوا مع بعضبيتكون بينهم رابطتين
+
+294
+00:22:07,930 --> 00:22:12,270
+بيبتديتين فبما أن هذا المراكب خلصار فيه رابطتين
+
+295
+00:22:12,270 --> 00:22:16,930
+بيبتديتين إذا أن العينة إذا فيها بيوريا بيوريا هذه
+
+296
+00:22:16,930 --> 00:22:20,630
+فيها رابطتين بيبتديتين حطيت عليها بيوري تريجين
+
+297
+00:22:20,630 --> 00:22:25,110
+بيجي الكبر كعادي بيرتبط مع الرابط البيبتديةبيكون
+
+298
+00:22:25,110 --> 00:22:29,350
+كلات Complex لونه violet بجيس شديد اللون هذا على
+
+299
+00:22:29,350 --> 00:22:33,650
+540 نانومتر وبيعطيني قراعه طب هذه القراع اللي طلعت
+
+300
+00:22:33,650 --> 00:22:37,670
+معايا مش هتكون لبروتين هتكون لمركب اسمه الـ Bioria
+
+301
+00:22:37,670 --> 00:22:41,630
+مابهناش يعرفني هل هي للـ Bioria ولا للبروتين يعني
+
+302
+00:22:41,630 --> 00:22:47,060
+ممكن تعطيني نتائج خاطئةإنه لحساسة لوجود مُركّبات
+
+303
+00:22:47,060 --> 00:22:51,560
+أخرى غير البروتين يعني هذه الطريقة مش خاصة بس
+
+304
+00:22:51,560 --> 00:22:55,140
+للبروتين إنها تعطيني قراءة بس للبروتين not
+
+305
+00:22:55,140 --> 00:23:00,120
+specific، specificity عندها قليلة إنها مش خاصة بس
+
+306
+00:23:00,120 --> 00:23:04,620
+للبروتين وإنما لأي مُركّب يحتوي على رابطتين
+
+307
+00:23:04,620 --> 00:23:10,530
+ببتديتين فأكتر زي البيوريأذن طريق العيوب الـ
+
+308
+00:23:10,530 --> 00:23:14,370
+Biorhythm test أنه ال sensitivity له قليلة و ال
+
+309
+00:23:14,370 --> 00:23:19,370
+specificity له قليلة أقل كمية قادرة على قياسها
+
+310
+00:23:19,370 --> 00:23:23,850
+قليلة ال specificity الخصوصية له برضه قليلة أنه
+
+311
+00:23:23,850 --> 00:23:29,950
+ممكن تختلط مواد أخرى و يعطيني إليها قراءة إيش هو
+
+312
+00:23:29,950 --> 00:23:33,530
+ال standard curve؟ ال standard curve هو عبارة عن
+
+313
+00:23:33,530 --> 00:23:37,190
+type of graph used for quantitative research
+
+314
+00:23:37,190 --> 00:23:43,540
+techniqueهو رسم بياني لمعرفة تركيز أي مادة مجهولة
+
+315
+00:23:43,540 --> 00:23:48,680
+رسم بياني برسمه عشان أعرف تركيز أي مادة مجهولة
+
+316
+00:23:48,680 --> 00:23:52,380
+standard care for protein concentration is often
+
+317
+00:23:52,380 --> 00:23:56,520
+created using non-concentration of bovine serum
+
+318
+00:23:56,520 --> 00:24:00,320
+أنه كيف أنا بدي استخدم هذا الرسم البياني لمعرفة
+
+319
+00:24:00,320 --> 00:24:04,120
+تركيز أي مادة مجهولة باستخدام حاجة اسمها standard
+
+320
+00:24:04,120 --> 00:24:09,430
+مرجعاللي هو عبارة عن البروتين بيكون معروف التركيز
+
+321
+00:24:09,430 --> 00:24:15,490
+هذا البروتين بيكون موجود في الجسم البروتين اللي
+
+322
+00:24:15,490 --> 00:24:22,150
+هنستخدمه هو الـ bovine serum
+
+323
+00:24:22,150 --> 00:24:28,910
+albumin وبروتين الألبومن من عجل صغيرالألبومين هو
+
+324
+00:24:28,910 --> 00:24:33,210
+عبارة عن سيرام البروتين اللي يترانسبورت الفاتي أسد
+
+325
+00:24:33,210 --> 00:24:42,310
+ومهم في تحفظ بلازمة BH هو بروتين موجود في الدم
+
+326
+00:24:42,310 --> 00:24:48,070
+يعمل كناقل لبعض المواد زي الفاتي أسد ويحافظ على
+
+327
+00:24:48,070 --> 00:24:53,940
+الـ BHفي الـ Protein Quantization Assay بوفاين
+
+328
+00:24:53,940 --> 00:24:58,900
+سيرام ألبومين تعمل كـ Reference Protein تستخدم
+
+329
+00:24:58,900 --> 00:25:04,060
+لإصلاح الـ Protein Standard Camp في فحوصات لمعرفة
+
+330
+00:25:04,060 --> 00:25:08,400
+تركيز البروتين نستخدم البروتين للـ Bovine Serum
+
+331
+00:25:08,400 --> 00:25:13,860
+البروتين كبروتين مرجع كستاندر كما ده بتكون عارفين
+
+332
+00:25:13,860 --> 00:25:18,540
+تركيزها ومن خلالها بتدعرف تركيز العينة اللي موجودة
+
+333
+00:25:18,540 --> 00:25:24,690
+عندههي مادة معروفة التركيز بستخدمها كمرجع لتقدير
+
+334
+00:25:24,690 --> 00:25:30,350
+تركيز مادة مجهولة الـ standard solution هو non
+
+335
+00:25:30,350 --> 00:25:34,330
+-concentration of sample الـ standard هو مادة
+
+336
+00:25:34,330 --> 00:25:40,910
+بتكون معروفة التركيز، مادة أنا عارفة تركيزهاالـ
+
+337
+00:25:40,910 --> 00:25:44,050
+standard curve حاكي هنا إن هو رسم بياني لمعرفة
+
+338
+00:25:44,050 --> 00:25:48,790
+تركيز أي مادة مجهولة بناء على إياش، بناء على وجود
+
+339
+00:25:48,790 --> 00:25:54,870
+standard مادة معروفة التركيزفجيت أنا ضروري أرسم
+
+340
+00:25:54,870 --> 00:25:58,170
+للـStandard Curve عشان أعرف التركيز العين المجهولة
+
+341
+00:25:58,170 --> 00:26:01,450
+اللي بتكون عندى كيف بدأ أعرف هذا التركيز؟ بناء على
+
+342
+00:26:01,450 --> 00:26:05,990
+وجود مادة معروف التركيز، مادة كمرجع المادة هذه
+
+343
+00:26:05,990 --> 00:26:09,410
+المعروفة التركيز اسمها الـStandard بتجيني من
+
+344
+00:26:09,410 --> 00:26:13,310
+الشركة هي بتجينها من الشركة بيكون معروف تركيزها
+
+345
+00:26:13,310 --> 00:26:17,190
+مكتوب عليها أن هو الـStandard وتركيزها هالجانإما
+
+346
+00:26:17,190 --> 00:26:21,350
+بتجيني من الشركة مخففة، منه هذا الـ standard نفسه
+
+347
+00:26:21,350 --> 00:26:25,450
+مخفف ومعمول منه عدة تخفيفات، كل تخفيف له تركيز
+
+348
+00:26:25,450 --> 00:26:28,850
+مختلف، مثلا الأولى بيكون مكتوب عليها إنه هي 5
+
+349
+00:26:28,850 --> 00:26:32,570
+milligram per meal، تانية 10 milligram per meal،
+
+350
+00:26:32,570 --> 00:26:36,510
+25 milligram per meal، 50 milligram per meal، إنه
+
+351
+00:26:36,510 --> 00:26:41,210
+مخفف واجازةأو بتجيني standard مركز مثلا مكتوب عليه
+
+352
+00:26:41,210 --> 00:26:44,510
+ان هو 2 milligram per ml و احنا مطلوب منه ان احنا
+
+353
+00:26:44,510 --> 00:26:49,270
+نعمل منه عدة تخفيفات عشان نشتغل عليه لو انا عملت
+
+354
+00:26:49,270 --> 00:26:53,150
+عدة تخفيفات كيف بدي احسب التخفي في الـ dilution
+
+355
+00:26:53,150 --> 00:27:02,430
+بالقانون C1V1 بتساوي C2V2الهو التركيز الأول تركيز
+
+356
+00:27:02,430 --> 00:27:05,890
+ال standard كده كان جبتلي الشركة أنه 2 milligram
+
+357
+00:27:05,890 --> 00:27:10,210
+في ال volume ضرب ال volume كده حجم اللي أنا أخدته
+
+358
+00:27:10,210 --> 00:27:14,310
+من هذا ال standard بساوي C2 التركيز اللي بعد ما
+
+359
+00:27:14,310 --> 00:27:17,860
+خففته هذا اللي أنا بتاعه عرفه مجهولدرب الـ Volume
+
+360
+00:27:17,860 --> 00:27:22,220
+2 اللي هو جدّيش أنا أخدت من الـ Distilled water و
+
+361
+00:27:22,220 --> 00:27:24,760
+جدّيش أخدت من الـ Standard يعني الـ Volume الـ
+
+362
+00:27:24,760 --> 00:27:28,260
+Total، الكلّي اللي هو حاجة جدّيش أنا أخدت من الـ
+
+363
+00:27:28,260 --> 00:27:32,840
+Standard و جدّيش أضفت عليه Distilled water، الـ
+
+364
+00:27:32,840 --> 00:27:35,960
+Volume الكلّي هذا هيوضح معنا إن شاء الله في المعمل
+
+365
+00:27:35,960 --> 00:27:38,540
+هنوضح كيف هذا القانون
+
+366
+00:27:40,920 --> 00:27:43,600
+How to calculate the concentration كيف نحسب
+
+367
+00:27:43,600 --> 00:27:47,460
+التركيز حكي هنا بناء على قانون الـPierre Lambert's
+
+368
+00:27:47,460 --> 00:27:52,680
+law اللي بيحكي إنه في علاقة بين تركيز المادة وكمية
+
+369
+00:27:52,680 --> 00:27:56,240
+الضوء الممتص إن الـconcentration of substance
+
+370
+00:27:56,240 --> 00:27:59,080
+directly proportional to the amount of light
+
+371
+00:27:59,080 --> 00:28:03,440
+absorbedانه تركيز المادة بيتناسب تناسط طرطي مع
+
+372
+00:28:03,440 --> 00:28:07,840
+كمية الضوء الممتص أهم نقطة هي النقطة الأخيرة في
+
+373
+00:28:07,840 --> 00:28:11,360
+هاي ال slide انه ال reference range for total
+
+374
+00:28:11,360 --> 00:28:18,540
+protein كمية البروتين الطبيعية هي من 66.6 ل 81.4
+
+375
+00:28:18,540 --> 00:28:24,460
+gram per liter او 6.6 ل 8.1 gram per deciliter
+
+376
+00:28:24,460 --> 00:28:30,420
+تختلف هنا الوحدات فركزوا على الوحداتكيف بدي أعمل
+
+377
+00:28:30,420 --> 00:28:33,860
+الـ Standard Curve اللي هو المخطط أو الرسم البياني
+
+378
+00:28:33,860 --> 00:28:37,100
+كيف بدي أعمله؟ كيف بدي يساعدني في معرفة تركيز
+
+379
+00:28:37,100 --> 00:28:41,020
+المادة المجهولة؟ حاجة الـ Standard Curve بينعمل
+
+380
+00:28:41,020 --> 00:28:44,540
+بناء على وجود مادة معروفة التركيز اسمها الـ
+
+381
+00:28:44,540 --> 00:28:49,000
+Standard إنه عندي مادة صار معروف تركيزها هذه
+
+382
+00:28:49,000 --> 00:28:52,160
+المادة سواء أجتني من الشركة بخففها وجهزة أو أنا
+
+383
+00:28:52,160 --> 00:28:56,580
+عملتلها تخفيفات وعرفت تركيز كل تخفيف المهم في
+
+384
+00:28:56,580 --> 00:29:01,720
+النهاية إنه عنديالـ Standard بيدّي التخفيفات مثلًا
+
+385
+00:29:01,720 --> 00:29:06,000
+أنا عندي أربعة standard واحد تخفيف وخمسة milligram
+
+386
+00:29:06,000 --> 00:29:11,820
+per ml milligram per ml وعشرة gram per ml وخمسة
+
+387
+00:29:11,820 --> 00:29:15,300
+وعشرين وخمسين هي عندي أربعة standard تخفيفات
+
+388
+00:29:15,300 --> 00:29:18,580
+مختلفة معروفة أن هي تركيزة التركيز الأول خمسة
+
+389
+00:29:18,580 --> 00:29:24,140
+والتاني عشرة التالت خمسة وعشرين الرابع خمسينإذا
+
+390
+00:29:24,140 --> 00:29:27,560
+عندي multiple sample with non-properties بياخدها
+
+391
+00:29:27,560 --> 00:29:31,980
+وبيعملها measure هذه العينات العدة اللي هي الـ
+
+392
+00:29:31,980 --> 00:29:37,460
+standard لمعروف خصائصها، معروف تركيزها بروح بجيسها
+
+393
+00:29:37,460 --> 00:29:41,840
+في جهاز الـ spectrophotometer باخدها، بقراها على
+
+394
+00:29:41,840 --> 00:29:45,320
+جهاز الـ spectrophotometer إيش بده أقرألها؟ بده
+
+395
+00:29:45,320 --> 00:29:49,220
+أقرألها الـ absorption هذا الجهاز بيجيس كمية الضوء
+
+396
+00:29:49,220 --> 00:29:53,090
+اللي بتمطسها العينة يعني بيجيس الـ absorptionبعد
+
+397
+00:29:53,090 --> 00:29:56,390
+ما حط طبعا ال reagent عليها بكون حاطة على هذا ال
+
+398
+00:29:56,390 --> 00:30:01,680
+standard باخده بحط عليه من ال reagent تمام؟هلجان
+
+399
+00:30:01,680 --> 00:30:05,920
+باخده بحط أول واحدة اللي هي تركيزها الخمسة أنا
+
+400
+00:30:05,920 --> 00:30:08,540
+عارفة تركيزها الخمسة بحطها في جهاز الـ
+
+401
+00:30:08,540 --> 00:30:12,640
+spectrophotometer و بقرأ ال absorption ال
+
+402
+00:30:12,640 --> 00:30:16,400
+absorption على فرض الأولى طلعت point واحد اتنين
+
+403
+00:30:16,400 --> 00:30:21,160
+تلاتة تمام بطلعها باخد التاني اللي هي تركيزها عشر
+
+404
+00:30:21,160 --> 00:30:24,860
+بقرأ ال absorption على فرض point واحد خمسة أربع
+
+405
+00:30:25,570 --> 00:30:30,090
+باخد 25 لتركيزها 25 وبقرألها الـ absorption على
+
+406
+00:30:30,090 --> 00:30:35,490
+فرد point 2 3 5 بعدين باخد لتركيزها 50 وبقرأ أعلى
+
+407
+00:30:35,490 --> 00:30:41,130
+فرد point 3 3تمام؟ هالجهة أنا بيصير عندي معروف
+
+408
+00:30:41,130 --> 00:30:44,690
+تركيز لهذه المادة وأخدتها قرأتها في جهاز
+
+409
+00:30:44,690 --> 00:30:47,310
+Spectrophotometer فصار عندي معروف اللي هي
+
+410
+00:30:47,310 --> 00:30:51,130
+absorption إذا عندي معروف تركيز، concentration و
+
+411
+00:30:51,130 --> 00:30:55,830
+absorption بجي برسم الـStandard Curve الـStandard
+
+412
+00:30:55,830 --> 00:31:00,850
+Curve كيف بدي أرسمه؟ بعبارة عن تقاطع صيني مع صعب
+
+413
+00:31:00,850 --> 00:31:06,710
+على الخط الصيني بيمثل الـconcentrationوالخط الصعدي
+
+414
+00:31:06,710 --> 00:31:10,790
+يمثل ال absorption is a standard curve الرسم
+
+415
+00:31:10,790 --> 00:31:15,630
+وعبارة عن تقاطع سيني وصعدي على الخط السيني بحط ال
+
+416
+00:31:15,630 --> 00:31:20,090
+concentration وعلى الصعدي بحط ال absorptionبحط
+
+417
+00:31:20,090 --> 00:31:25,210
+تقسيمة لخط الصيني وخط الصيني وبجيب أرسم مثلا أول
+
+418
+00:31:25,210 --> 00:31:27,870
+نقطة اللي هي خمسة من الصيني من ال concentration
+
+419
+00:31:27,870 --> 00:31:31,910
+تركيزها كان خمسة مع تقاطع من ال absorption طلعت
+
+420
+00:31:31,910 --> 00:31:35,510
+point واحد اتنين تلاتة بحط النقطة الأولى اللي هي
+
+421
+00:31:35,510 --> 00:31:38,890
+تقاطع خمسة مع point واحد اتنين تلاتة النقطة
+
+422
+00:31:38,890 --> 00:31:41,730
+التانية اللي هي تركيزها عشرة مع point واحد خمسة
+
+423
+00:31:41,730 --> 00:31:46,430
+أربعة بحط النقطة التالتة خمسة وعشرين تركيزها من ال
+
+424
+00:31:46,430 --> 00:31:51,200
+concentration مع point اتنين تلاتة خمسةالرابع
+
+425
+00:31:51,200 --> 00:31:56,540
+تركيزها الخمسين مع point تلاتة تلاتة بحط النقطة
+
+426
+00:31:56,540 --> 00:32:01,300
+وبعدين بوصل النقاط أكبر عدد من النقاط بحيث يمثل خط
+
+427
+00:32:01,300 --> 00:32:07,000
+مستقيم برسم خط مستقيم يمر بأكبر عدد من النقاط بوصل
+
+428
+00:32:07,000 --> 00:32:12,300
+النقاط مع بعض على بعض بحيث يمثل خط مستقيم بتكون
+
+429
+00:32:12,300 --> 00:32:18,280
+عندى من ضمن هدول في عندى عينة مجهولة التركيزفي
+
+430
+00:32:18,280 --> 00:32:21,480
+عندي عينة ماخدينها من مريض مثلًا لأعرف تركيز
+
+431
+00:32:21,480 --> 00:32:24,720
+البروتين فيها بحط عليها الـReagent و باخدها على
+
+432
+00:32:24,720 --> 00:32:28,760
+جهاز الـSpectrophotometer بقرا إليها absorption،
+
+433
+00:32:28,760 --> 00:32:32,360
+انتصار في هذه المادة بيصير عندي معروف إليها
+
+434
+00:32:32,360 --> 00:32:37,360
+الـabsorption بس مش معروف التركيز باجي على التقاط
+
+435
+00:32:37,360 --> 00:32:40,940
+عصاد اللي بيمثل الـabsorption على فرض العينة مثلًا
+
+436
+00:32:40,940 --> 00:32:47,990
+طلعت point1.7 بأجي على point 1.7 من الـ absorption
+
+437
+00:32:47,990 --> 00:32:53,230
+بوصل خط اللي وصولا إلى الخط المستقيم اللي في الوسط
+
+438
+00:32:53,230 --> 00:32:58,910
+وبنزل عمود على الخط الساني وبشوف جداش .. وين وين
+
+439
+00:32:58,910 --> 00:33:03,430
+وصلت عنديا تركيز وبكتب تركيز هذه العينة المجهولة
+
+440
+00:33:04,260 --> 00:33:09,080
+أنا عارف إنه صعب عليكم تفهموه هنا لكن هنوضحه في
+
+441
+00:33:09,080 --> 00:33:11,880
+المعمل ونورسمه إن شاء الله على الصجورة ونوضح كيف
+
+442
+00:33:11,880 --> 00:33:17,810
+بينعمل الـ Standard Kشايفين هنا الرسم اللي تحت
+
+443
+00:33:17,810 --> 00:33:22,110
+اللي هو تقاطع سيني مع صعدي خط السيني بيمثل الـ
+
+444
+00:33:22,110 --> 00:33:25,790
+concentration والخط الصعدي بيمثل الـ absorption
+
+445
+00:33:25,790 --> 00:33:30,350
+يكون عندي مواد جياني معروف تركيزها كل واحدة إلاها
+
+446
+00:33:30,350 --> 00:33:34,030
+concentration مختلف وabsorption على الخط الصعدي
+
+447
+00:33:34,030 --> 00:33:36,890
+أنا أخدت هذه العينات وقرأتها في جهاز
+
+448
+00:33:36,890 --> 00:33:40,860
+Spectrophotometer وطلع معايا absorptionهتبقى اشي
+
+449
+00:33:40,860 --> 00:33:45,160
+بمثل اول نقطة مثلا على فرقة خمسة من الصيني ال
+
+450
+00:33:45,160 --> 00:33:48,300
+concentration و من الصادر طلع ال absorption الها
+
+451
+00:33:48,300 --> 00:33:52,800
+point واحد نات بقى بحط نقطة النقطة اللي بعدها لكل
+
+452
+00:33:52,800 --> 00:33:56,360
+عينة جداش تركيزها ماجداش ال absorption بحط النقطة
+
+453
+00:33:56,360 --> 00:34:02,940
+بوصل خط مستقيم يمر بمعظم النقاطفي عندي عيناة بتكون
+
+454
+00:34:02,940 --> 00:34:07,600
+unknown عيناة مثلا ماخدها من مريض وفيها بروتين بدي
+
+455
+00:34:07,600 --> 00:34:10,920
+أعرف كمية هذه البروتين بحط عليها من الـ reagent زي
+
+456
+00:34:10,920 --> 00:34:14,700
+الـ standard بعملها معاملة الـ standard بحط عليها
+
+457
+00:34:14,700 --> 00:34:17,020
+reagent و بعدين باخدها على جهاز الـ
+
+458
+00:34:17,020 --> 00:34:20,580
+spectrophotometerوفي الجهاز بيعطيني absorption
+
+459
+00:34:20,580 --> 00:34:24,800
+بيعطيني امتصاد باجي على الخط الصعدي اللي هي ال
+
+460
+00:34:24,800 --> 00:34:27,500
+absorption أنا عارفة ال absorption لهذه العينة
+
+461
+00:34:27,500 --> 00:34:31,720
+قرأتها على الجهاز وعلى فرض طلعت point 1 3 5 يعني
+
+462
+00:34:31,720 --> 00:34:36,270
+ده كلمة signal الخط الأحمرعلى فرض هي أنها طلعت الـ
+
+463
+00:34:36,270 --> 00:34:39,790
+absorption بوصل خط إلى الخط المستقيم اللي في
+
+464
+00:34:39,790 --> 00:34:43,670
+المنتصف وبسقط عمود من عند النقطة اللي وصلت عندها
+
+465
+00:34:43,670 --> 00:34:48,790
+بسقط عمود على الصانع وإن ما سقط هذا العمود بيكون
+
+466
+00:34:48,790 --> 00:34:53,570
+هذا الـ concentration هذا عبارة عنتركيز هذه المادة
+
+467
+00:34:53,570 --> 00:34:57,430
+عفارة طلعت مثلا بين خمسين وخمسة وعشرين بقدر انه
+
+468
+00:34:57,430 --> 00:35:01,750
+ممكن تكون هي خمسة واربعين ممكن تكون خمسة وتلاتين
+
+469
+00:35:01,750 --> 00:35:08,330
+فشوف وين وقعت بالظبط اذا هذا هو تركيز المادة وهي
+
+470
+00:35:08,330 --> 00:35:13,190
+عندنا هنا مخطط تاني على الخط الصيني بحط الـ
+
+471
+00:35:13,190 --> 00:35:16,250
+Concentration وهنا على الخط الصدي بحط الـ
+
+472
+00:35:16,250 --> 00:35:21,270
+Absorptionكل عينة أنا قرأتها جداش إلها absorption
+
+473
+00:35:21,270 --> 00:35:25,410
+مع جداش concentration بحط نقطة، النقطة اللي بعلى،
+
+474
+00:35:25,410 --> 00:35:31,050
+بعدين بحط خط مستقيم بيمر بمعظم النقاط، والعينة
+
+475
+00:35:31,050 --> 00:35:35,890
+المجهولة بشوف عندهاجدّش هي الـ absorption بكون أنا
+
+476
+00:35:35,890 --> 00:35:38,370
+قرأها على الجهاز وعارفة إيه لها الـ absorption
+
+477
+00:35:38,370 --> 00:35:43,030
+جدّش الإمتصاص طلع بوصل على الخط المستقيم وبسقط
+
+478
+00:35:43,030 --> 00:35:47,150
+عمودة على الـ concentration وبشوف وين يطلع تركيزها
+
+479
+00:35:47,150 --> 00:35:52,250
+عند أي رقم هيك بكون أنا عارفة تركيز هذه المادة
+
+480
+00:35:52,250 --> 00:35:57,390
+وبرضه هنرجع نوضح في المعمل كيف المخطط نرسمه وكيف
+
+481
+00:35:57,390 --> 00:36:05,170
+نعمل على إيجاد تركيز أي مادة مشهولةهنا عندنا مجموع
+
+482
+00:36:05,170 --> 00:36:09,550
+من التعريفات عندنا تعريف الـ sensitivity حكينا
+
+483
+00:36:09,550 --> 00:36:14,390
+عنها sensitivity هي أقل كمية أو أقل تركيز من
+
+484
+00:36:14,390 --> 00:36:19,190
+الانالايت قادر لفحصها لقياسه أقل كمية بيقدر يجيسها
+
+485
+00:36:19,190 --> 00:36:22,990
+بنسميها sensitivity lowest amount of analyte in
+
+486
+00:36:22,990 --> 00:36:27,110
+sample which can be detectedأما ال specificity
+
+487
+00:36:27,110 --> 00:36:31,990
+اللي هي الخصوصية الخصوصية هي قدرة الفحص إنه يكون
+
+488
+00:36:31,990 --> 00:36:36,730
+خاص أو فريد فقط لـ Analyte معين اللي أنا ببحث عنه
+
+489
+00:36:36,730 --> 00:36:41,710
+is the ability to assess uniquely vocally the
+
+490
+00:36:41,710 --> 00:36:45,490
+analyte in the presence of components which may be
+
+491
+00:36:45,490 --> 00:36:49,150
+expected to be present إنه عندي مثلا عينة أنا عندي
+
+492
+00:36:49,150 --> 00:36:52,720
+هذا الفحص بدي فحص السكرطب هى العين اللى أنا أخدتها
+
+493
+00:36:52,720 --> 00:36:56,780
+فيها عدة مكونات من ضمنها السكر لكن فيه حاجات تانية
+
+494
+00:36:56,780 --> 00:37:01,080
+فيه مثلا البروتين وفيه carbohydrate سكر لكن أنا
+
+495
+00:37:01,080 --> 00:37:04,600
+هذا الفحص إذا كان عنده specificity عالية فهو بس
+
+496
+00:37:04,600 --> 00:37:08,600
+قادر على قياس السكر مالوش دخل في المكونات التانية
+
+497
+00:37:08,600 --> 00:37:13,520
+مايجسنيش غير السكر بس قدرة الفحص على أنه يرتبط مع
+
+498
+00:37:13,520 --> 00:37:17,240
+الانالايت اللى أنا ببحث عنه مع حاجة واحدة بدون
+
+499
+00:37:17,240 --> 00:37:22,040
+الاختلاعات مع المكونات الأخرىالـ Linearity
+
+500
+00:37:22,040 --> 00:37:37,000
+Linearity of Analytical Procedure هي علاقة
+
+501
+00:37:37,000 --> 00:37:40,600
+الترضية اللي حكينا عنها بالـ absorption و الـ
+
+502
+00:37:40,600 --> 00:37:43,990
+concentrationهي ال directly proportional اللي هي
+
+503
+00:37:43,990 --> 00:37:48,450
+العلاقة الخطية معناها العلاقة الترضية اللي بين
+
+504
+00:37:48,450 --> 00:37:55,150
+تركيز المادة وكمية الضوء اللي بيمتصها ايه شغلنا
+
+505
+00:37:55,150 --> 00:37:59,270
+لليوم؟ اليوم هو فحصنا ال virus test لنشتغل virus
+
+506
+00:37:59,270 --> 00:38:03,370
+test ولكن ك quantitative اللي بدي اقرأه في جهاز
+
+507
+00:38:03,370 --> 00:38:06,990
+spectrophotometer و بالنهاية بدي اعمل standard
+
+508
+00:38:06,990 --> 00:38:10,330
+curve بس اعمل هذا ال standard curve
+
+509
+00:38:15,570 --> 00:38:26,610
+تركيز المادة المجهولة تركيز
+
+510
+00:38:26,610 --> 00:38:27,770
+المادة المجهولة تركيز المادة المجهولة تركيز المادة
+
+511
+00:38:27,770 --> 00:38:32,350
+المجهولة تركيز المادة المجهولة تركيز المادة
+
+512
+00:38:32,350 --> 00:38:40,390
+المجهولة تركيز المادة المجهولة تركيز المادة
+
+513
+00:38:40,390 --> 00:38:46,400
+المجهولةفكيف بدي أخففه؟ بنيعمل من هذا الـ Standard
+
+514
+00:38:46,400 --> 00:38:51,700
+6 تيوبات بحكي للتيوب الأول، احنا بشو بنخفف؟ بنحط
+
+515
+00:38:51,700 --> 00:38:54,600
+من المادة المركزة، بنخففها في الـ distilled water
+
+516
+00:38:54,600 --> 00:38:57,860
+فبحكي للتيوب الأول، حطي عليه من الـ distilled
+
+517
+00:38:57,860 --> 00:39:02,560
+water خمسة من عشرة مل فلجيت احنا بشو بنستخدم لما
+
+518
+00:39:02,560 --> 00:39:08,420
+نحط؟ بنستخدم بايبت والبايبت هي بالمايكرون كل واحد
+
+519
+00:39:08,420 --> 00:39:14,710
+مل بيعطيني ألف مايكرونيعني الخمسة مل تعطيني خمسمية
+
+520
+00:39:14,710 --> 00:39:18,710
+مايكرون كل هدول الأرقام اللي بالجدول بالمل بتحولهم
+
+521
+00:39:18,710 --> 00:39:24,450
+لمايكرون كاب كل واحد مل بيعطيني الف مايكرون خمسة
+
+522
+00:39:24,450 --> 00:39:28,370
+من عشرة مل يعني خمسمية مايكرون تطلع فالـ tube
+
+523
+00:39:28,370 --> 00:39:32,890
+الأول بحكيلي نحط عليها خمسمية مايكرون من الـ
+
+524
+00:39:32,890 --> 00:39:36,130
+distilled water و بدناش نحط عليها من ال protein
+
+525
+00:39:36,130 --> 00:39:40,960
+standard، نحط صفرطب أنا ماحطتش ال protein خالص
+
+526
+00:39:40,960 --> 00:39:43,560
+ماجددش تركيز ال protein في ال tube الأول
+
+527
+00:39:43,560 --> 00:39:49,200
+concentration له سفر أكيد سفر أنا عندي ال tube
+
+528
+00:39:49,200 --> 00:39:53,600
+الأول بس حقيت عليه distill water ماحطيتش عليه من
+
+529
+00:39:53,600 --> 00:39:59,600
+ال standard فإذا تركيزه هيكون سفر مافيش تركيز له
+
+530
+00:39:59,600 --> 00:40:05,240
+سفر milligram per ml لأنه ماحطناش عليه ولا إشي من
+
+531
+00:40:05,240 --> 00:40:10,030
+ال standard«تيوب» رقم 2 بدي أحط عليها من الـ
+
+532
+00:40:10,030 --> 00:40:14,730
+«دستيل واتر» 4 من 10 مل يعني 400 مايكرون ومن الـ
+
+533
+00:40:14,730 --> 00:40:20,690
+«بروتين ستاندرد» بدي أحط عليها 100 مايكرون 1 من 10
+
+534
+00:40:20,690 --> 00:40:24,350
+مل يعني 100 مايكرون لذلك هذه خففة الـ «ستاندرد»
+
+535
+00:40:24,350 --> 00:40:28,270
+الأول إن حطيت 4 من 10 «دستيل واتر» يعني 400
+
+536
+00:40:28,270 --> 00:40:32,450
+مايكرون على الـ «ستاندرد» الـ «بروتين ستاندرد» 1
+
+537
+00:40:32,450 --> 00:40:38,760
+من 10 مل يعني 100 مايكرونقدش تركيزه بيطلع بعد
+
+538
+00:40:38,760 --> 00:40:42,500
+تخفيف 4 من 10؟ كيف حسبتي التركيز؟ رجزوا معايا فيها
+
+539
+00:40:42,500 --> 00:40:47,880
+حكينا بنحسب تركيز الـ Standard المخفف بناء على
+
+540
+00:40:47,880 --> 00:40:55,340
+قانونC1 V1 سواء C2 V2 خلّينا في تيوب رقم 2 ركزوا
+
+541
+00:40:55,340 --> 00:41:00,140
+معايا على تيوب رقم 2 كيف بده أحسب التركيز؟ C1 اللي
+
+542
+00:41:00,140 --> 00:41:04,160
+هو التركيز للـ Standard قبل التخفير احنا حكينا إنه
+
+543
+00:41:04,160 --> 00:41:07,360
+جاينا من الشركة الـ Standard جدا تركيزه 2
+
+544
+00:41:07,360 --> 00:41:13,740
+milligram برميل، إذا C1 هي 2 milligram برميلدرب V1
+
+545
+00:41:13,740 --> 00:41:18,800
+الـ V1 هذا كداش أنا أخدت من الـ Standard كداش حطيت
+
+546
+00:41:18,800 --> 00:41:21,080
+على تيوب اتنين من الـ Standard البروتين الـ
+
+547
+00:41:21,080 --> 00:41:24,920
+Standard هكالي واحد من عشرة مل يعني مائة مايكرو
+
+548
+00:41:24,920 --> 00:41:29,580
+اذاً C1 اللي هو تركيزه الأصلي تركيز الـ Standard
+
+549
+00:41:29,580 --> 00:41:36,320
+كداش كان عندي اتنين درب V1 V1 هي كمية الحجم اللي
+
+550
+00:41:36,320 --> 00:41:39,940
+أنا أخدته من الـ Standard كداش أخدت مية اذا صار
+
+551
+00:41:39,940 --> 00:41:45,800
+عندي اتنين درب ميةبساوي C2، إيش هي C2؟ هو التركيز
+
+552
+00:41:45,800 --> 00:41:49,120
+اللي أنا بتعرفه، جدّاش صارت بعد، جدّاش تركيزها
+
+553
+00:41:49,120 --> 00:41:53,680
+بعد، فهي و سيبنا مجهولة، لسه بديها، V2 هو الـ
+
+554
+00:41:53,680 --> 00:41:57,020
+Total، الحجم الكل، جدّاش حطيت من الـDistilled
+
+555
+00:41:57,020 --> 00:42:00,640
+water مع الـStandard، في Tube 2، جدّاش حطيت، حكي
+
+556
+00:42:00,640 --> 00:42:03,420
+أنا حطينا من الـDistilled water، 4 من 10 ميلي،
+
+557
+00:42:03,420 --> 00:42:07,550
+يعني 400 مايكروومن الـ Standard حطيت 1 من 10 مل
+
+558
+00:42:07,550 --> 00:42:10,950
+يعني 100 مايكرون يعني 400 مايكرون و100 مايكرون
+
+559
+00:42:10,950 --> 00:42:16,550
+يعني الـ total كله قداش 500 مايكرون، إذا الـ V2 هي
+
+560
+00:42:16,550 --> 00:42:21,550
+500 مايكرون، إذا صرت بعرف C1 2 عبارة عن 2 ميلي
+
+561
+00:42:21,550 --> 00:42:28,990
+جرام، V1 هي 100 مايكرون، C2 بتديها، V2 هي 500
+
+562
+00:42:28,990 --> 00:42:34,730
+مايكرونمن المعادلة 2 ضرب 100 على 500 بتطلع التركيز
+
+563
+00:42:34,730 --> 00:42:39,530
+4 من 10 فهمنا كيف جيبنا الـ Concentration لـ Tube
+
+564
+00:42:39,530 --> 00:42:47,410
+2 من القانون C1 V1 سواء C2 V2 منهي C1 هو تركيز الـ
+
+565
+00:42:47,410 --> 00:42:50,830
+Standard اللي بدون تخفيف الأصل اللي جانب الشركة
+
+566
+00:42:50,830 --> 00:42:56,050
+جدش كان 2 milligram per minuteالـ V1 هي التركيز
+
+567
+00:42:56,050 --> 00:42:58,570
+اللي أنا أخدته من الـ Standard كده أخدتي حجم من
+
+568
+00:42:58,570 --> 00:43:03,250
+الـ Standard؟ أخدنا 100 مايكرو الـ C2 هذا بتديها
+
+569
+00:43:03,250 --> 00:43:07,390
+مجهولة الـ V2 هي عبارة عن الحجم الكل ديستل واتر
+
+570
+00:43:07,390 --> 00:43:11,970
+زائد للـ Standard أخدت 400 مع 100 يعني 500 مايكرو
+
+571
+00:43:11,970 --> 00:43:16,690
+بيطلع عند الـ concentration 4 من 10نجي على تيوب 3
+
+572
+00:43:16,690 --> 00:43:20,950
+تيوب 3 حطيت عليها 3 من 10 مل من الـ distilled
+
+573
+00:43:20,950 --> 00:43:25,290
+water يعني 300 مايكرون و 2 من 10 مل من الـ
+
+574
+00:43:25,290 --> 00:43:30,150
+standard يعني 200 مايكرون عليكوا تحسبولي إيها الـ
+
+575
+00:43:30,150 --> 00:43:33,790
+concentration و تسلمونيها هو التركيز أنا طلع 8 من
+
+576
+00:43:33,790 --> 00:43:38,170
+10 إليها بدي أكم تعرفولي كيف طلع 8 من 10 بناء على
+
+577
+00:43:38,170 --> 00:43:43,450
+القانون زي ما حسبنا لتيوب 2 لنحسب ل3ولا أربعة ولا
+
+578
+00:43:43,450 --> 00:43:49,110
+خمسة وتعطوني الإجابة تسلموني الواجب إنه تحسبولي
+
+579
+00:43:49,110 --> 00:43:54,850
+لتيوب رقم تلاتة واربعة وخمسة تيوب رقم تلاتة بيحكي
+
+580
+00:43:54,850 --> 00:43:57,850
+إننا نحط عليها تلاتة من عشرة distilled water يعني
+
+581
+00:43:57,850 --> 00:44:02,230
+300 مايكرون واتنين من عشرة من البروتين ال standard
+
+582
+00:44:02,230 --> 00:44:08,380
+يعني 200 مايكرونوبعد أن تيوب رقم 4 بتحط عليها 2 من
+
+583
+00:44:08,380 --> 00:44:11,640
+10 ميلي من الـ distilled water يعني 2 مايكرون
+
+584
+00:44:11,640 --> 00:44:15,060
+ديستل واتر تلاتة من عشرة من البروتين ال standard
+
+585
+00:44:15,060 --> 00:44:21,380
+يعني 300 مايكرونتيوب رقم 5 بدي أحط عليها 1 من 10
+
+586
+00:44:21,380 --> 00:44:25,100
+ملي من الـ distilled water 1 من 10 ملي يعني 100
+
+587
+00:44:25,100 --> 00:44:29,060
+مايكرون ومن الـ protein standard بدي أحط 4 من 10
+
+588
+00:44:29,060 --> 00:44:34,760
+ملي يعني 400 مايكرون تيوب رقم 6 بديش أحط عليها
+
+589
+00:44:34,760 --> 00:44:38,540
+distilled water بس بدي أحط عليها من الـ protein
+
+590
+00:44:38,540 --> 00:44:42,920
+standardخمسة من عشرة ملي، يعني خمسمائة مايكرون،
+
+591
+00:44:42,920 --> 00:44:46,100
+إذا انتيوب رقم ست، أنا ماعملتلهااش تخفيف، حطيتها
+
+592
+00:44:46,100 --> 00:44:49,120
+زي مياه، خدت بس ماحطش عليها دستل ووتر، يعني
+
+593
+00:44:49,120 --> 00:44:53,020
+ماخففتاش، يعني من الأصل، الـ standard الأصل، حطيت
+
+594
+00:44:53,020 --> 00:44:58,080
+عليها خمسمائة مايكرون، فتركيزها جداش هيكون، أكيد
+
+595
+00:44:58,080 --> 00:45:02,380
+مدام ماخففتاش، بجي تركيزها نفس تركيز الأصلي، اللي
+
+596
+00:45:02,380 --> 00:45:08,800
+هو اتنين ملي جرام per mil، ماتغيرش تركيزها،أخر
+
+597
+00:45:08,800 --> 00:45:13,500
+تيوب أحنا نجيب سبعة تيوبات، ستة منهم هم تخفيفات
+
+598
+00:45:13,500 --> 00:45:19,040
+للـStandard والسبعة هي Unknown، هي عينة مريض بتاعه
+
+599
+00:45:19,040 --> 00:45:22,640
+عارف كمية البروتين فيها، بديش أحط عليها ولايش من
+
+600
+00:45:22,640 --> 00:45:25,740
+الـDistilled water ولا من الـStandard، وإنما من
+
+601
+00:45:25,740 --> 00:45:29,960
+العينة اللي جاية عندي تحط خمسة من عشرة مل، يعني
+
+602
+00:45:29,960 --> 00:45:35,240
+خمسمية مايكروم هل أنا عارف كمية تركيزها، كمية
+
+603
+00:45:35,240 --> 00:45:39,740
+البروتين لهذا المريض؟ لأبنحكي لبعد ما نحضر هدول
+
+604
+00:45:39,740 --> 00:45:43,840
+التيوبات كلهم بنحط واحد مل من الـ bioretary agent
+
+605
+00:45:43,840 --> 00:45:48,860
+على كل التيوبات بنحط واحد مل من الـ bioretary
+
+606
+00:45:48,860 --> 00:45:53,940
+agent على كل التيوبات و أعمل mix بعدين بتعملها
+
+607
+00:45:53,940 --> 00:45:59,900
+incubation لمدة عشرين منه تخليها مدة عشرين دقيقة
+
+608
+00:45:59,900 --> 00:46:06,340
+على الـ room تنبرجها عشان يصير ارتفاعبعدين بدي أخد
+
+609
+00:46:06,340 --> 00:46:09,920
+كل تيوب من هذه التيوبات و أقرأها على جهاز
+
+610
+00:46:09,920 --> 00:46:14,780
+Spectrophotometer على طول موجي خمسمية و أربعين
+
+611
+00:46:14,780 --> 00:46:19,260
+نانو متر، إيش هيطلعلي؟ هيطلعلي absorb، يعني كل
+
+612
+00:46:19,260 --> 00:46:23,710
+تيوب بحطبيعطيني قراءة absorption الـ tube اللي
+
+613
+00:46:23,710 --> 00:46:28,550
+بعدها بيعطيني absorption وهكذا للسبعة tubeات فصار
+
+614
+00:46:28,550 --> 00:46:31,510
+عندي لل tubeات من واحد لستة معروف الـ
+
+615
+00:46:31,510 --> 00:46:35,550
+concentration ومعروف عندي absorption بس ال tube
+
+616
+00:46:35,550 --> 00:46:39,790
+رقم سبعة ال unknown بس معروف عندي absorption قرأت
+
+617
+00:46:39,790 --> 00:46:43,410
+على جهاز الـ spectrophotometer وعرفت ال absorption
+
+618
+00:46:43,410 --> 00:46:50,350
+ماقدرتش أعرف ال concentrationتمام هالجيت بعمل ال
+
+619
+00:46:50,350 --> 00:46:53,530
+standard curve برسم ال curve اللي هو عبارة عن
+
+620
+00:46:53,530 --> 00:46:57,450
+مضحنة حكاية سيني وصادي على الخط السيني بدي أرسم ال
+
+621
+00:46:57,450 --> 00:47:01,550
+concentration وعلى الصادي بدي أرسم ال absorption
+
+622
+00:47:02,360 --> 00:47:07,940
+بحط النقاط لـ Standard و بعدين بطلّع تركيز المادة
+
+623
+00:47:07,940 --> 00:47:11,640
+المجهولة أنا عارفة إيه لها absorption و بدي أطلّع
+
+624
+00:47:11,640 --> 00:47:15,440
+تركيزها من الـ standard gap إن شاء الله هذا كله
+
+625
+00:47:15,440 --> 00:47:21,460
+هيوضح معناه بالشغل و لما نوضحها بالمعنى بيحكيلي في
+
+626
+00:47:21,460 --> 00:47:25,820
+حاجة اسمها blank solution هو solution محلول بيحتوي
+
+627
+00:47:25,820 --> 00:47:29,140
+على little to no alkaline usually used to
+
+628
+00:47:29,140 --> 00:47:33,060
+calibrate an instrumentإنه هذا هو عبارة عن محلول،
+
+629
+00:47:33,060 --> 00:47:36,480
+بحطش فيه ولا أي شيء، ولا standard ولا sample، بس
+
+630
+00:47:36,480 --> 00:47:40,560
+بحط فيه الـreagent عشان الـreagent إيه اللون، إذا
+
+631
+00:47:40,560 --> 00:47:44,200
+فاتبين الـbio-reagent، لونه أزرق فجيت أنا لو هذا
+
+632
+00:47:44,200 --> 00:47:47,460
+اللون أنا حاططه على جهاز الـSpectrum، هيقرألي هذا
+
+633
+00:47:47,460 --> 00:47:52,960
+اللون الأزرقفهي يتلخبط ويعطيني قراءة زيادة من
+
+634
+00:47:52,960 --> 00:47:56,520
+اللون الأزرق الـ Reagent نفسه واللون بتاع التفاعل
+
+635
+00:47:56,520 --> 00:48:00,260
+مش الجهاز اللي بيقرأ كمية اللون فهيقرأ لون الـ
+
+636
+00:48:00,260 --> 00:48:05,920
+Reagent العصني وهيقرأ لون التفاعل فأنا بحط الـ
+
+637
+00:48:05,920 --> 00:48:09,720
+Tube بس تحتوي على الـ Reagent عشان أصفر عليها
+
+638
+00:48:09,720 --> 00:48:14,020
+الجهازإن الجهاز يقرأ كمية هذا اللون و بعد هي
+
+639
+00:48:14,020 --> 00:48:17,980
+يخصومها كأنه بده يخصومها من التيوبات التانية زي
+
+640
+00:48:17,980 --> 00:48:21,300
+إذا خدتوا في المقدمة كيف نوزن على الميزان كيف
+
+641
+00:48:21,300 --> 00:48:25,540
+بنستخدم الميزان بحط ال cup مثلا اللي بدي أوزن فيه
+
+642
+00:48:25,540 --> 00:48:28,660
+بدي أحط فيه المادة أول أجي بحط ال cup عشان إيه
+
+643
+00:48:28,660 --> 00:48:34,740
+الوزن بصفر عليه و بعدين بحط المادة عشان يشيل وزنة
+
+644
+00:48:34,740 --> 00:48:40,800
+ال cupوأنا بصفر على لون الـ region عشان بعد هيك
+
+645
+00:48:40,800 --> 00:48:46,240
+أشيل لون الـ region عشان يعملني calibration فالمرة
+
+646
+00:48:46,240 --> 00:48:50,620
+الجاي لما نشتغل هذه التجربة بدنا معانا ورق رسم
+
+647
+00:48:50,620 --> 00:48:55,260
+بياني عشان نرسم عليه الـ standard curve
+
diff --git a/PL9fwy3NUQKwZ1Q7zOg1ObgufPMjRI3WY1/vIoMDwX27cM.srt b/PL9fwy3NUQKwZ1Q7zOg1ObgufPMjRI3WY1/vIoMDwX27cM.srt
new file mode 100644
index 0000000000000000000000000000000000000000..01549ccfc50216169b847a1593f70e04bc5e50e1
--- /dev/null
+++ b/PL9fwy3NUQKwZ1Q7zOg1ObgufPMjRI3WY1/vIoMDwX27cM.srt
@@ -0,0 +1,1386 @@
+1
+00:00:00,000 --> 00:00:04,660
+العمل ليه لأ المعمل؟ المعمل بدنا نشتغل فيه
+
+2
+00:00:04,660 --> 00:00:08,220
+determination و vitamin C اللي هو ascorbic acid
+
+3
+00:00:08,220 --> 00:00:12,200
+concentration by redox titration with potassium 
+
+4
+00:00:12,200 --> 00:00:18,290
+iodate بنحدد كمية vitamin C اللي هو الـ ascorbic
+
+5
+00:00:18,290 --> 00:00:24,210
+acid من خلال عملية المعايرة الـ titration بواسطة
+
+6
+00:00:24,210 --> 00:00:28,250
+بوتاسيوم ايودات ايش الـ titrant اللي بدي استخدمه
+
+7
+00:00:28,250 --> 00:00:32,470
+الـ titrant هو البوتاسيوم ايودات، لذا البوتاسيوم
+
+8
+00:00:32,470 --> 00:00:36,070
+ايودات هو عبارة عن الـ titrant فاليوم أنا بدي أجيب
+
+9
+00:00:36,070 --> 00:00:38,910
+pure ascorbic acid، ascorbic acid فيتامين،
+
+10
+00:00:38,910 --> 00:00:43,350
+ascorbic acid مش مضاعف عليه ولا أي شيء، هو pure، و
+
+11
+00:00:43,350 --> 00:00:46,750
+أعمله معايرة مع بوتاسيوم أيودات، أشوف كده كميته،
+
+12
+00:00:46,750 --> 00:00:49,250
+أحسبله الـmolarity، و أحسبله الـmillimole، و
+
+13
+00:00:49,250 --> 00:00:55,830
+أحسبله الـwater، و بدي أعمل كمان مرة معايرة تانية على
+
+14
+00:00:55,830 --> 00:00:59,770
+عصير برتقال طبيعي بدي أجيب عصير برتقال طبيعي ليه؟
+
+15
+00:00:59,770 --> 00:01:03,030
+بيحتوي على vitamin C ال ascorbic acid بدي أشوف جدّش
+
+16
+00:01:03,030 --> 00:01:07,990
+كمية ال ascorbic acid الموجودة فيه ومعايرة تالتة
+
+17
+00:01:07,990 --> 00:01:14,510
+بدي أجيب عصير برتقال صناعي عصير برتقال صناعي أشوف
+
+18
+00:01:14,510 --> 00:01:18,950
+كمية ال ascorbic acid الموجودة فيه vitamin C جدّش
+
+19
+00:01:18,950 --> 00:01:24,700
+موجود فيه إذا ال objective to determine vitamin C
+
+20
+00:01:24,700 --> 00:01:28,420
+by potassium iodate titration أن أنا بدي أحدد كمية
+
+21
+00:01:28,420 --> 00:01:32,620
+vitamin C باستخدام potassium iodate titration وين
+
+22
+00:01:32,620 --> 00:01:38,500
+بدي أحدده في البيور ascorbic acid في البيور وين in
+
+23
+00:01:38,500 --> 00:01:42,640
+artificial orange juice وين in natural orange
+
+24
+00:01:42,640 --> 00:01:48,170
+juice الـ principle أو خلينا نحكي عن المعادلة .. عن
+
+25
+00:01:48,170 --> 00:01:52,870
+ال .. عن التجربة ككل Vitamin C حكينا أن هو
+
+26
+00:01:52,870 --> 00:01:57,090
+antioxidant يعني reducing agent بيسموها خاصية أي
+
+27
+00:01:57,090 --> 00:02:03,390
+تخزين يوائل الصيادلة باسم reducing agent لأن هو ضد
+
+28
+00:02:03,390 --> 00:02:08,590
+الأكسدة يعني عامل مختزل يعني بختزل غيره هو يصير له
+
+29
+00:02:08,590 --> 00:02:12,640
+أكسدة Suitable method for the determination of
+
+30
+00:02:12,640 --> 00:02:15,420
+vitamin C quantities is titration with potassium
+
+31
+00:02:15,420 --> 00:02:20,320
+iodate بحكي لأفضل طريقة لتحديد كمية vitamin C عشان
+
+32
+00:02:20,320 --> 00:02:23,980
+أعرف كمية vitamin C هي أن تعمل عملية معايرة
+
+33
+00:02:23,980 --> 00:02:28,400
+titration مع ال potassium iodate potassium iodate
+
+34
+00:02:28,400 --> 00:02:32,850
+اللي هو ال KIO3 اللي بستخدمه كـ titrant Iodometric
+
+35
+00:02:32,850 --> 00:02:37,850
+titration أنه بتصير عملية معايرة في بداية المعايرة
+
+36
+00:02:37,850 --> 00:02:43,450
+الـ analyte بيتفاعل بيصير له reduce with excess
+
+37
+00:02:43,450 --> 00:02:50,210
+iodine I- ل I2 which turn blue in the presence of
+
+38
+00:02:50,210 --> 00:02:54,450
+starch بعدين ال I2 هاي بتتفاعل مع ال starch مع
+
+39
+00:02:54,450 --> 00:02:59,470
+النشا كـ indicator يعني وبيعطيني اللون، بيتغير اللون
+
+40
+00:03:01,270 --> 00:03:05,850
+عشان نفهم كل شيء بيصير فيها و نحكي هذا الكلام بدنا
+
+41
+00:03:05,850 --> 00:03:09,370
+نكون فاهمين إنه ال buret أنا بدي أعبيها بـ titrant
+
+42
+00:03:09,370 --> 00:03:14,660
+ايش هو الـ titrant اللي بدي يكون موجود هو ال KIO3 إذا
+
+43
+00:03:14,660 --> 00:03:18,660
+وظيفتي الـ KIO3 هو titrant تمام هو البوتاسيوم
+
+44
+00:03:18,660 --> 00:03:21,920
+ايودات هذا بعبيه في البيور هجيب ال flask ال
+
+45
+00:03:21,920 --> 00:03:24,460
+volumetric flask اللي بدي أعمل فيها معايرة بدي أحط
+
+46
+00:03:24,460 --> 00:03:27,760
+فيها المادة اللي أنا بدي أعيرها بحط فيها كمية من
+
+47
+00:03:27,760 --> 00:03:31,080
+ال ascorbic acid اللي أنا بدي أعيره إذا بيور أو
+
+48
+00:03:31,080 --> 00:03:35,900
+إذا orange juice artificial أو natural بدي أحط أول
+
+49
+00:03:35,900 --> 00:03:39,960
+مرة مثلا على البيور بحط اسكوربيك أسيد في ال flask
+
+50
+00:03:39,960 --> 00:03:45,300
+بنضيف عليها كمان potassium iodide potassium iodide
+
+51
+00:03:45,300 --> 00:03:49,760
+اللي هو ال KI potassium iodide هو ال KI بضيفه في
+
+52
+00:03:49,760 --> 00:03:53,660
+ال flask على ال ascorbic acid بيساهم في التفاعل و
+
+53
+00:03:53,660 --> 00:03:59,680
+عندنا هنشوف كده و بضيف كمية من HCl ليش ال HCl
+
+54
+00:03:59,680 --> 00:04:05,180
+بيعطيني ظروف حمضية للتفاعل و بضيف كمية من ال starch
+
+55
+00:04:05,180 --> 00:04:09,860
+اللي بيشتغل كـ indicator إذا التفاعل مكوناته
+
+56
+00:04:09,860 --> 00:04:14,980
+potassium iodide iodate اللي هو ال KIO3 هذا لل
+
+57
+00:04:14,980 --> 00:04:19,560
+titrant المعينة وفيه عندنا potassium iodide اللي هو
+
+58
+00:04:19,560 --> 00:04:23,920
+ال KI هذا بيساهم في التفاعل وفيه عندنا HCL اللي
+
+59
+00:04:23,920 --> 00:04:28,270
+بيعطينا ظروف حمضية فيه ال starch بستخدمه كـ
+
+60
+00:04:28,270 --> 00:04:31,950
+indicator و ال ascorbic acid اللي بتعمله معايرة
+
+61
+00:04:31,950 --> 00:04:36,210
+عشان نفهم ال principle فجيت كيف اللي بدي اشتغل
+
+62
+00:04:36,210 --> 00:04:41,390
+التفاعل ال potassium iodate اللي هو ال KIO3 ركزوا
+
+63
+00:04:41,390 --> 00:04:47,550
+هان ال potassium iodate اللي هو ال KIO3 بيتفاعل مع
+
+64
+00:04:47,550 --> 00:04:55,170
+ال KI اللي هو ال potassium iodide KIO3 زائد KI زائد
+
+65
+00:04:55,170 --> 00:05:01,370
+potassium iodide زائد HCL في وجود ظروف حمضية وسط
+
+66
+00:05:01,370 --> 00:05:08,180
+حمضية بيتكون عندي molecular iodine I2 إذن أول ما
+
+67
+00:05:08,180 --> 00:05:11,260
+يبدأ التفاعل أبدأ أنزل من ال بيوريت ال potassium
+
+68
+00:05:11,260 --> 00:05:14,440
+iodate بفتح ال بيوريت و أنزل potassium iodate ال
+
+69
+00:05:14,440 --> 00:05:17,980
+titrant بيجي هذا ال potassium iodate ال KIO3
+
+70
+00:05:17,980 --> 00:05:21,640
+بيتفاعل مع ال KI مع ال potassium iodide اللي أنا
+
+71
+00:05:21,640 --> 00:05:26,000
+ضفتها في ال flask في وجود HCl ظروف حمضية بيعطيني
+
+72
+00:05:26,000 --> 00:05:32,650
+molecular iodine I2 ال I2 هاي ال Molecular Iodine
+
+73
+00:05:32,650 --> 00:05:37,490
+بس تتكون بتتفاعل مع ال Ascorbic Acid مع الشيء اللي
+
+74
+00:05:37,490 --> 00:05:40,010
+أنا بدي أعمله معايرة مع ال Ascorbic Acid
+
+75
+00:05:40,770 --> 00:05:44,970
+الأسكوربيك أسيد هو reducing agent يعني بيختزل غيره
+
+76
+00:05:44,970 --> 00:05:50,010
+لما يتفاعل مع ال أيودين بيعمل reducing ل I2
+
+77
+00:05:50,010 --> 00:05:57,110
+وبيحوله إلى reduced form اللي هو I- تمام؟ وهو
+
+78
+00:05:57,110 --> 00:06:02,650
+الأسكوربيك أسيد بيصير له أكسدة وبيتحول إلى ديهيدرو
+
+79
+00:06:02,650 --> 00:06:08,370
+أسكوربيك أسيد أو بنسميه oxidized ascorbic acid إذن
+
+80
+00:06:08,370 --> 00:06:12,230
+أول ما يبدأ التفاعل بقى نزل من الـBurette titrant
+
+81
+00:06:12,230 --> 00:06:16,570
+اللي هو الـ Potassium Iodide الـ KIO3 بتتفاعل مع
+
+82
+00:06:16,570 --> 00:06:20,890
+Potassium Iodide اللي هي الـ KI في وجود HCl
+
+83
+00:06:20,890 --> 00:06:26,480
+بيعطيني Molecular Iodine I2 بتيجي ال I2 بتتفاعل مع
+
+84
+00:06:26,480 --> 00:06:31,160
+ال Ascorbic Acid بيصير عملية أكسدة و اختزال ال I2
+
+85
+00:06:31,160 --> 00:06:36,080
+بتتحول ل I- و ال Ascorbic Acid بتتحول إلى Oxidized
+
+86
+00:06:36,080 --> 00:06:41,580
+Ascorbic Acid أو Dehydro Ascorbic Acid الجدّ بس
+
+87
+00:06:41,580 --> 00:06:45,640
+يتفاعل ال Iodine مع ال Ascorbic Acid بيضلوا يتفاعل
+
+88
+00:06:45,640 --> 00:06:48,440
+ال Iodine مع Ascorbic Acid ويحاولوا لـ Dehydro
+
+89
+00:06:48,440 --> 00:06:52,500
+Ascorbic Acid ويظل يستمر التفاعل لحد ما يخلص ال
+
+90
+00:06:52,500 --> 00:06:57,980
+Ascorbic Acid مدام ما خلص ال Ascorbic Acid بيجي ال
+
+91
+00:06:57,980 --> 00:07:02,700
+Iodine هذا اللي ضايل بيتفاعل مع ال starch اللي
+
+92
+00:07:02,700 --> 00:07:09,360
+موجود عشان يعطيني لون permanent dark blue color لون
+
+93
+00:07:09,360 --> 00:07:14,180
+أزرق غامق و يكون permanent هذا اللون يعني أنا بعمل
+
+94
+00:07:14,180 --> 00:07:19,000
+mix لل flask ما يتغيرش اللون يظل مستمر اللون إذا
+
+95
+00:07:19,000 --> 00:07:22,940
+ظلّ مستمر اللون هاي بتكون ال end point عندها
+
+96
+00:07:22,940 --> 00:07:27,380
+خلاص ووقف التفاعل خلص التفاعل بقرأ ال buret لوين
+
+97
+00:07:27,380 --> 00:07:31,940
+وصل حجمه و بحمل حسابات هنحكي عنها لـ بعدين إذن
+
+98
+00:07:31,940 --> 00:07:37,040
+التفاعل أول ما يبدأ بيتفاعل KIO3 مع KI في وجود HCl
+
+99
+00:07:37,040 --> 00:07:41,400
+بيعطيني موليكولار Iodine I2 I2 بتتفاعل مع Ascorbic
+
+100
+00:07:41,400 --> 00:07:45,440
+Acid بيصير عملية أكسدة واختزال إلى Dehydro
+
+101
+00:07:45,440 --> 00:07:50,390
+Ascorbic Acid و I- هذا الجهاز بيخلص كل الـ ascorbic acid 
+
+102
+00:07:50,390 --> 00:07:54,430
+الـ iodine اللي ضايل ال I2 موليكولار أيودين هذا
+
+103
+00:07:54,430 --> 00:07:59,530
+بيتفعل مع ال starch مع ال indicator و بيتغير اللون
+
+104
+00:07:59,530 --> 00:08:04,910
+وبيعطيني dark blue color تمام و لازم يكون permanent
+
+105
+00:08:04,910 --> 00:08:10,010
+هذا اللون يعني لون دائم مستمر مش أنه يكون مؤقت
+
+106
+00:08:10,010 --> 00:08:15,610
+يجي ويختفي فعشان .. عشان الـ iodine أضمن أنه كمية
+
+107
+00:08:15,610 --> 00:08:20,870
+ال I2 بتكون كميات كبيرة بده أحط ال KI في ال flask
+
+108
+00:08:20,870 --> 00:08:25,250
+البوتاسيوم أيودايد بده أحط منه كميات زائدة in
+
+109
+00:08:25,250 --> 00:08:30,450
+excess amount فاحط ال KI ب excess amount بكميات
+
+110
+00:08:30,450 --> 00:08:34,900
+زائدة عشان أضمن استمرارية التفاعل ويظل ال KI
+
+111
+00:08:34,900 --> 00:08:39,480
+يتفاعل مع ال KIO3 ويعطيني molecular Iodine و ال
+
+112
+00:08:39,480 --> 00:08:43,280
+Iodine بيتفاعل مع ال Ascorbic Acid لعند ما يخلص و
+
+113
+00:08:43,280 --> 00:08:47,080
+يزيد من هذا ال Iodine بيتفاعل مع ال starch مش أنه ال
+
+114
+00:08:47,080 --> 00:08:51,200
+Iodine يكون كميته قليلة ال Potassium Iodide يكون
+
+115
+00:08:51,200 --> 00:08:56,360
+كميته قليلة، ما يعطينيش Iodine بكميات كفاية، هيخلص
+
+116
+00:08:56,360 --> 00:08:59,380
+ال Iodine قبل أن يخلص ال Ascorbic Acid، بالتالي
+
+117
+00:08:59,380 --> 00:09:02,400
+ما فيش Iodine زيادة مش هيتفاعل مع ال starch ولا
+
+118
+00:09:02,400 --> 00:09:06,990
+هيغير اللون، فبيحكي لـ Potassium Iodide ال KI
+
+119
+00:09:06,990 --> 00:09:11,010
+بنحطه ب excess amount بكميات زيادة عشان أضمن
+
+120
+00:09:11,010 --> 00:09:15,630
+استمرارية التفاعل ويعطيني molecular iodine بكميات
+
+121
+00:09:15,630 --> 00:09:19,910
+زايدة بحيث إنه يتفاعل مع ال ascorbic acid يظل
+
+122
+00:09:19,910 --> 00:09:24,230
+يتفاعل لحد ما يخلص ال ascorbic acid يجيب له و هو
+
+123
+00:09:24,230 --> 00:09:28,590
+بعدين يروح يتفاعل مع ال starch ويعطيني اللون ال
+
+124
+00:09:28,590 --> 00:09:34,180
+permanent dark blue color هلجيت؟ كل slide هي محصلة
+
+125
+00:09:34,180 --> 00:09:38,240
+اللي حاكيناه ايش مكتوب؟ potassium iodate بنستخدمه
+
+126
+00:09:38,240 --> 00:09:42,360
+كـ titrant اللي هو ال KIO3 بنستخدمه كـ titrant بنضيفه
+
+127
+00:09:42,360 --> 00:09:46,320
+على ال Ascorbic acid وعلى strong acid على flask
+
+128
+00:09:46,320 --> 00:09:49,000
+هذا ال potassium iodate بيكون موجود في ال burette
+
+129
+00:09:49,000 --> 00:09:53,880
+بنزله على flask بتحتوي على Ascorbic acid وعلى
+
+130
+00:09:53,880 --> 00:09:58,570
+strong acid اللي هو ال HCl و potassium iodide Ki
+
+131
+00:09:58,570 --> 00:10:02,330
+اجيت ال potassium iodate أول ما ينزل من ال buret
+
+132
+00:10:02,330 --> 00:10:06,170
+على ال flask بيتفاعل مع مين؟ بيتفاعل مع HCl و
+
+133
+00:10:06,170 --> 00:10:10,610
+potassium iodide بيتفاعل مع Ki في وجود HCl ايش
+
+134
+00:10:10,610 --> 00:10:14,850
+بيعطيني؟ ال molecular iodine ال I2 ال molecular
+
+135
+00:10:14,850 --> 00:10:19,030
+iodine هذا بيتفاعل مع ال ascorbic acid بيعمل له
+
+136
+00:10:19,030 --> 00:10:24,000
+أكسدة بيكون له oxidized ascorbic acid اللي اسمه
+
+137
+00:10:24,000 --> 00:10:30,900
+dehydroascorbic acid إضافة الحمض بيحكي لي ليش فقط
+
+138
+00:10:30,900 --> 00:10:35,000
+ليه تعمل acidic condition بتعطيني ظروف حمضية إذا
+
+139
+00:10:35,000 --> 00:10:40,100
+ال acid وظيفته ظروف حمضية potassium iodide must be
+
+140
+00:10:40,100 --> 00:10:43,220
+added in excess to keep iodine dissolved بيحكي لي
+
+141
+00:10:43,220 --> 00:10:47,100
+ال KI اللي هي ال potassium iodide بنحطها بكميات
+
+142
+00:10:47,100 --> 00:10:52,550
+زائدة عشان أضمن أنها تعطيني molecular iodine بكميات
+
+143
+00:10:52,550 --> 00:10:56,650
+كبيرة يضلوا يتفاعل مع ال ascorbic acid لعند ما
+
+144
+00:10:56,650 --> 00:11:01,150
+يخلص ولما يخلص يروح يتفاعل كمان هذا ال iodine
+
+145
+00:11:01,150 --> 00:11:06,470
+لزيادة مع ال starch و يعطيني ال end point once all
+
+146
+00:11:06,470 --> 00:11:09,410
+the ascorbic acid has been consumed بس يخلص ال
+
+147
+00:11:09,410 --> 00:11:13,690
+ascorbic acid ال excess من ال iodine اللي ضايل من
+
+148
+00:11:13,690 --> 00:11:18,630
+ال iodine من ال I2 بيروح بيتفاعل مع ال starch
+
+149
+00:11:18,630 --> 00:11:22,530
+وبيعطيني ال end point بيحكي لي excess iodine قال
+
+150
+00:11:22,530 --> 00:11:26,490
+جت ال iodine ايش لونه؟ لونه لون بني فال excess من ال
+
+151
+00:11:26,490 --> 00:11:29,350
+iodine إذا يضلوا ال iodine بتفاعل مع كل ال ascorbic
+
+152
+00:11:29,350 --> 00:11:33,180
+acid وخلص ال ascorbic acid لون ال solution بيكون
+
+153
+00:11:33,180 --> 00:11:36,940
+لونه بني فممكن أعتمد على هذا اللون البني و أحكي
+
+154
+00:11:36,940 --> 00:11:39,960
+إنه خلاص مدام صار لون بني يعني ال iodine صار
+
+155
+00:11:39,960 --> 00:11:43,240
+بكميات زيادة يجي هذه ال end point وما أضيفش ال
+
+156
+00:11:43,240 --> 00:11:47,060
+starch أن يكون هذا ال iodine كـ it is on indicator
+
+157
+00:11:47,060 --> 00:11:49,880
+و أنه هستخدمه كـ indicator و خلاص و أواجه ما أحطش
+
+158
+00:11:49,880 --> 00:11:54,400
+starch بيحكي لي هو بنفع أنه يستخدمه كـ indicator
+
+159
+00:11:54,400 --> 00:11:58,000
+لكن في هذه التجربة هنا difficult اني أحدد ال end
+
+160
+00:11:58,000 --> 00:12:03,290
+point باستخدام ال iodine واللون صعب علي ان أحدد ال
+
+161
+00:12:03,290 --> 00:12:07,570
+end point باستخدام iodine لون ال iodine ليه؟ لأن
+
+162
+00:12:07,570 --> 00:12:11,110
+احنا حاولنا أن نعمل عملية معايرة مع عصير برتقال
+
+163
+00:12:11,110 --> 00:12:15,350
+عصير البرتقال لونه برتقالي قريب من لون ال iodine
+
+164
+00:12:15,350 --> 00:12:20,020
+فممكن يصير عندي التباس أنه هل هذا من ال iodine اللي
+
+165
+00:12:20,020 --> 00:12:25,700
+زاد لونه ولا اللون أصلاً عصير البرتقال فالأفضل أني
+
+166
+00:12:25,700 --> 00:12:30,060
+بيحكي لي بنضيف ال starch عشان يتفاعل مع ال iodine
+
+167
+00:12:30,060 --> 00:12:33,860
+وبيعطيني لون blue ال starch بيتفاعل مع ال
+
+168
+00:12:33,860 --> 00:12:39,060
+iodine و بيعطيني لون blue لون أزرق هذا اللون لازم
+
+169
+00:12:39,060 --> 00:12:42,440
+يكون permanent بيحكي لي the first permanent trace
+
+170
+00:12:42,440 --> 00:12:45,920
+of dark blue black color due to starch iodine
+
+171
+00:12:45,920 --> 00:12:49,300
+complex ما دام أضفت ال starch مع الايودين بيكون لي
+
+172
+00:12:49,300 --> 00:12:53,510
+complex لونه blue black color بيكون لونه اللون
+
+173
+00:12:53,510 --> 00:12:57,230
+الأزرق اللون الأزرق الغامق اللون أزرق غامق اللون
+
+174
+00:12:57,230 --> 00:12:57,770
+أزرق غامق اللون أزرق غامق اللون أزرق غامق اللون
+
+175
+00:12:57,770 --> 00:13:00,610
+أزرق غامق اللون أزرق غامق اللون أزرق غامق اللون
+
+176
+00:13:00,610 --> 00:13:00,750
+أزرق غامق اللون أزرق غامق اللون أزرق غامق اللون
+
+177
+00:13:00,750 --> 00:13:03,750
+أزرق غامق اللون أزرق غامق اللون أزرق غامق اللون
+
+178
+00
+
+201
+00:14:42,580 --> 00:14:49,340
+كل واحد مول من الـ KIO3 بيعطيني تلاتة مول من
+
+202
+00:14:49,340 --> 00:14:54,800
+الـ I2 من الـ Iodine إذا كل واحد مول من الـ KIO3
+
+203
+00:14:54,800 --> 00:14:59,340
+بيعطيني تلاتة مول من الـ Iodine كل واحد Iodine من
+
+204
+00:14:59,340 --> 00:15:03,780
+هدول بيتفعل مع الـ Ascorbic Acid أنا بدي أصل
+
+205
+00:15:03,780 --> 00:15:10,260
+علاقة بين الـ KIO3 و الـ Ascorbic Acid بدي علاقة بين الـ
+
+206
+00:15:10,260 --> 00:15:14,540
+KIO3 و الـ ascorbic acid كيف بدي أجيب هذه العلاقة؟
+
+207
+00:15:14,540 --> 00:15:18,780
+كل واحد mol من الـ KIO3 بيعطيني تلاتة mol من ال
+
+208
+00:15:18,780 --> 00:15:22,220
+iodine بعدين كل واحد mol من الـ iodine بيعطيني واحد
+
+209
+00:15:22,220 --> 00:15:25,320
+mol من الـ ascorbic acid يعني التلاتة mol من ال
+
+210
+00:15:25,320 --> 00:15:30,970
+iodine مع تلاتة ascorbic acid مع واحد KIO3 تمام
+
+211
+00:15:30,970 --> 00:15:36,350
+فواحد مول من الـ KIO3 
+
+212
+00:15:36,350 --> 00:15:41,050
+بتعطيني تلاتة مول ascorbic acid هذه علاقة مهمة في
+
+213
+00:15:41,050 --> 00:15:47,530
+حساباتنا لقدام كل واحد مول من الـ KIO3 يعطيني تلاتة
+
+214
+00:15:47,530 --> 00:15:53,100
+مول من الـ ascorbic acid هنا الـ structure مش مطلوب
+
+215
+00:15:53,100 --> 00:15:56,880
+منها للاسكوربيك أسيد ولا الديهيدرو أسكوربيك أسيد
+
+216
+00:15:56,880 --> 00:16:02,900
+بيحكيلي هنا ملاحظة الـ ink point بأوصلها لما يتحول
+
+217
+00:16:02,900 --> 00:16:08,080
+اللون لـ permanent dark blue color مركز على كلمة
+
+218
+00:16:08,080 --> 00:16:11,500
+permanent ومن وين أجت هذا الـ permanent dark blue
+
+219
+00:16:11,500 --> 00:16:14,540
+color؟ من وين أجه هذا اللون؟ بسبب التكوين complex
+
+220
+00:16:14,540 --> 00:16:18,890
+بين الـ starch و الـ iodine During an iodometric
+
+221
+00:16:18,890 --> 00:16:21,870
+titration intermediate dark blue iodine starch
+
+222
+00:16:21,870 --> 00:16:25,930
+complex may form imminently before the iodine
+
+223
+00:16:25,930 --> 00:16:29,770
+react with ascorbic acid بيحكيلي أثناء المعايرة
+
+224
+00:16:29,770 --> 00:16:35,520
+ممكن يتكون عندك اللون الـ dark blue بسبب اصطدام ال
+
+225
+00:16:35,520 --> 00:16:39,300
+iodine مع الـ starch كونه complex لكن سرعان ما يفكه
+
+226
+00:16:39,300 --> 00:16:43,400
+و يروح الـ iodine يتفاعل مع الـ ascorbic acid لأنه لسه
+
+227
+00:16:43,400 --> 00:16:47,800
+موجود فلو اللون هذا اختفى disappear مع الـ mixing
+
+228
+00:16:47,800 --> 00:16:51,580
+لما تعملي mix يختفي إذا هذي مش الـ end point أنا
+
+229
+00:16:51,580 --> 00:16:57,180
+بكونش وصلت للـ end point Iodometric Titration
+
+230
+00:16:57,180 --> 00:17:02,320
+موليكولير Iodine بيحيل الموليكولير I2 لكن هذا
+
+231
+00:17:02,320 --> 00:17:06,700
+بيتفاعل مع الـ Ascorbic Acid هو ضعيف الذائبية
+
+232
+00:17:06,700 --> 00:17:12,780
+ذائبيته ضعيفة slightly سوليومي فبيتفاعل مع الـ KI
+
+233
+00:17:12,780 --> 00:17:17,760
+الـ I2 هاي بس تنتج بتتفاعل هي حقيقة في التفاعل ال
+
+234
+00:17:17,760 --> 00:17:22,120
+I2 ذائبيتها ضعيفة صعب تتفاعل مع الـ Ascorbic Acid
+
+235
+00:17:22,120 --> 00:17:26,750
+فماشي اللي بيصير في التفاعل بتحول الـ I2I بتتفاعل
+
+236
+00:17:26,750 --> 00:17:31,710
+مع الـ Potassium Iodide مع الـ Ki و بتعطي مركب
+
+237
+00:17:31,710 --> 00:17:38,450
+اسمه Triiodide I-3 هذا الـ Triiodide ذائب هو اللي
+
+238
+00:17:38,450 --> 00:17:42,890
+بيتفاعل مع الـ Ascorbic Acid حقيقة احنا حكينا عن
+
+239
+00:17:42,890 --> 00:17:46,310
+المعادلة ان المونيكولار I2I هو اللي بتفاعل مع الـ
+
+240
+00:17:46,310 --> 00:17:51,930
+Ascorbic Acid عشان بدناش نفرق لكن هو حقيقة الـ I2I
+
+241
+00:17:52,170 --> 00:17:57,350
+ذقبيتها ضعيفة تتفاعل مع الـ Ki و بتعطيني Triiodide
+
+242
+00:17:57,350 --> 00:18:01,630
+هو اللي بيتفاعل مع الـ Ascorbic Acid الـ starch is
+
+243
+00:18:01,630 --> 00:18:05,770
+used as indicator إيش وظيفة الـ starch هان ك
+
+244
+00:18:05,770 --> 00:18:09,170
+indicator ككاشف بيعطيني اللون و بيعطيني الـ end
+
+245
+00:18:09,170 --> 00:18:13,230
+point بيعمل complex مع الـ iodine و بيعطيني اللون
+
+246
+00:18:15,100 --> 00:18:18,740
+طيب التجربة كيف خطوات إجراء التجربة؟ التجربة هذه
+
+247
+00:18:18,740 --> 00:18:23,040
+بدي أعمل عملية معايرة على تلت مرة أول مرة بدي
+
+248
+00:18:23,040 --> 00:18:26,660
+أعملها على pure ascorbic acid تاني مرة على عصير
+
+249
+00:18:26,660 --> 00:18:32,060
+برتقال طبيعي و تالت مرة على عصير برتقال صناعي هلجت
+
+250
+00:18:32,060 --> 00:18:34,700
+إن الـ buret في التلت حالات الـ buret إيش بدي
+
+251
+00:18:34,700 --> 00:18:37,300
+أعبيها؟ بدي أعبيها بالـ titrant اللي هو البوتاسيوم
+
+252
+00:18:37,300 --> 00:18:41,840
+ايودات فأنا بدي أخد الـ buret و أعبيها للآخر بـ KIO3
+
+253
+00:18:41,840 --> 00:18:46,770
+اللي هو بوتاسيوم ايودات الـ Flask بدي أضع عليها في
+
+254
+00:18:46,770 --> 00:18:50,970
+أول واحد في الـ pure ascorbic acid بدي أضع 25ml من
+
+255
+00:18:50,970 --> 00:18:57,310
+الـ pure ascorbic acid على 4ml HCl على 3ml من ال
+
+256
+00:18:57,310 --> 00:19:03,490
+starch و 5ml من البوتاسيوم أيودان بنضع 25ml
+
+257
+00:19:03,490 --> 00:19:10,130
+Ascorbic Acid على 4ml HCl على 3ml Starch على 5ml
+
+258
+00:19:10,130 --> 00:19:17,050
+Ki وبعدين بفتح صمام لبيوريت أكيد استخدمته في
+
+259
+00:19:17,050 --> 00:19:22,740
+التحليلية في المعمل بفتح الصمام مجرد مافتح الصمام
+
+260
+00:19:22,740 --> 00:19:26,780
+بيبدأ ينزل الـ KIO3 نقطة نقطة على الـ flask و هو
+
+261
+00:19:26,780 --> 00:19:30,880
+بينزل و أنا بعمل mix بنزل و بعمل mix متى ما اتكون
+
+262
+00:19:30,880 --> 00:19:35,000
+اللون اللي هو dark blue color و يكون permanent
+
+263
+00:19:35,000 --> 00:19:40,300
+اللون ثابت و بيختفيش بوقف المعايرة بسكر الصمام و
+
+264
+00:19:40,300 --> 00:19:44,680
+خلاص هنا انا وصلت للـ end point بسجل قراءة البوريت
+
+265
+00:19:44,680 --> 00:19:48,840
+اللي هو الـ volume بتاع الـ KIO3 لوين وصلت البوريت
+
+266
+00:19:48,980 --> 00:19:53,480
+هذا الـ volume من الـ KIO3 أنا جداش استهلكت بسجل
+
+267
+00:19:53,480 --> 00:19:58,280
+عندي الـ volume تمام بعد هيك بدي أعيد التجربة بدي
+
+268
+00:19:58,280 --> 00:20:02,160
+أكب الـ flask و أعيد التجربة مرة تانية على natural
+
+269
+00:20:02,160 --> 00:20:07,800
+orange juice بدي أجيب برضه الـ buret فيها KIO3 و ال
+
+270
+00:20:07,800 --> 00:20:12,140
+flask بدي أحط فيها 25 مل من الـ ascorbic acid على 4
+
+271
+00:20:12,140 --> 00:20:18,450
+مل HCl 3 من الـ star و 5 من الـ KI تمام و بفتح صمام
+
+272
+00:20:18,450 --> 00:20:22,810
+الـ buret بيبدأ ينزل عندي من الـ KIO3 على ال
+
+273
+00:20:22,810 --> 00:20:26,410
+solution على الـ flask و بعمل mix بضلني اعمل mix
+
+274
+00:20:26,410 --> 00:20:29,470
+لحد ما يظهر عندي اللون الـ permanent dark blue
+
+275
+00:20:29,470 --> 00:20:36,360
+color و بسجل عندى خلاص وصل اللون بسكر الصمام و بقرأ
+
+276
+00:20:36,360 --> 00:20:40,340
+الـ volume للـ KIO3 وين وصل و بسجل عندى هذا ال
+
+277
+00:20:40,340 --> 00:20:45,280
+volume للـ KIO3 في حالة الـ natural juice بعدين بعمل
+
+278
+00:20:45,280 --> 00:20:49,460
+كمان مرة نفس الخطوات بس بدل الـ natural juice بحط
+
+279
+00:20:49,460 --> 00:20:54,090
+25 ml من الـ artificial juice و زي ما هم أربعة من ال
+
+280
+00:20:54,090 --> 00:20:59,370
+hcl تلاتة من الـ starch خمسة من الـ ki و بفتح الصمم
+
+281
+00:20:59,370 --> 00:21:03,610
+بعمل معايرة مدى ما اتحول اللون لـ bare من الـ dark
+
+282
+00:21:03,610 --> 00:21:09,270
+blue color بسكر الصمم هاد و بسجل عندي النتيجة هسجل
+
+283
+00:21:09,270 --> 00:21:13,090
+النتيجة اللي زهرت عندي اللي هو الـ volume اللي أنا
+
+284
+00:21:13,090 --> 00:21:21,280
+قرعته هاي مش مهمة مش مطلوبة منها هو هاي ملخص
+
+285
+00:21:21,280 --> 00:21:25,200
+الفيتامينات إيش هي كم من الـ vitamin عندنا هلجت
+
+286
+00:21:25,200 --> 00:21:29,360
+احنا بعد هاي التجربة بعد ما نسجل الـ نتائج بنسجل
+
+287
+00:21:29,360 --> 00:21:34,840
+قراءة الـ KIO3 بنبدأ بعملية حسابات بنحسب الـ molarity
+
+288
+00:21:34,840 --> 00:21:40,220
+و بنحسب الـ millimol في حالة artificial و في حالة
+
+289
+00:21:40,220 --> 00:21:44,320
+الـ natural
+
+290
+00:21:44,320 --> 00:21:49,180
+و في حالة الـ بيون مش عارف واضحين هال ولا لأ، يعني
+
+291
+00:21:49,180 --> 00:21:52,760
+كبرتهم، أنا كتبتهم، هلجت احنا من المعادلة اللي
+
+292
+00:21:52,760 --> 00:21:56,780
+تاو حكينا عنها، المعادلة اللي تاو حكينا عنها،
+
+293
+00:21:56,780 --> 00:22:01,440
+حكينا إن 1 مول من الـ KIO3 بيتفعل مع 3 مول
+
+294
+00:22:01,440 --> 00:22:06,640
+Ascorbic Acid، 1 مول KIO3 يعطيني 3 مول Ascorbic
+
+295
+00:22:06,640 --> 00:22:10,030
+Acid لو بنحولها لـ millimol أخدته في التحليلية أنه
+
+296
+00:22:10,030 --> 00:22:15,250
+بتصير 1 على N millimol KiO3 1 على N millimol
+
+297
+00:22:15,250 --> 00:22:20,490
+ascorbic acid و N هي عدد المولاها هلجيت؟ 1 على N
+
+298
+00:22:20,490 --> 00:22:25,030
+millimol KiO3 اللي هي 1 على 1 1 على N من millimol
+
+299
+00:22:25,030 --> 00:22:30,100
+ascorbic acid هي 1 على 3 الـ millimole ايش بتساوي
+
+300
+00:22:30,100 --> 00:22:34,120
+بتساوي الـ molarity ضرب الـ volume فبدي احول الـ KIO3
+
+301
+00:22:34,120 --> 00:22:37,180
+بدل ما هي millimole بدي اكتب فيها molarity ضرب
+
+302
+00:22:37,180 --> 00:22:41,660
+volume من الـ KIO3 بيساوي تلت الـ millimole من ال
+
+303
+00:22:41,660 --> 00:22:45,540
+ascorbic acid أنا بدي احسب الـ millimole لل
+
+304
+00:22:45,540 --> 00:22:49,140
+ascorbic acid فبيطلع عندى من هذا القانون بس ننتج
+
+305
+00:22:49,140 --> 00:22:52,780
+ان الـ millimole للـ ascorbic acid ايش بتساوي تلاتة
+
+306
+00:22:52,780 --> 00:22:59,460
+ضرب الـ molarity ضرب الـ volume تمام تلاتة ضرب الـ
+
+307
+00:22:59,460 --> 00:23:03,480
+molarity ضرب الـ volume إذا أول قانون أول حساب ده
+
+308
+00:23:03,480 --> 00:23:09,000
+نحسبه هو الـ millimol للأسكار بكهسين اللي هو قانونه
+
+309
+00:23:09,000 --> 00:23:12,320
+تلاتة في الـ molarity في الـ volume بدي أحسبه هذا
+
+310
+00:23:12,320 --> 00:23:17,260
+مرة للبيور و مرة للـ natural و مرة للـ artificial
+
+311
+00:23:17,260 --> 00:23:20,720
+إيش بدي أعوض الـ millimol للأسكار بكهسين مثلا في
+
+312
+00:23:20,720 --> 00:23:24,270
+حالة البيور؟ إيش بدي أكتب؟ بتساوي تلاتة ضرب ال
+
+313
+00:23:24,270 --> 00:23:30,210
+molarity ثابت الـ molarity هي 0.00038 هي تمانية
+
+314
+00:23:30,210 --> 00:23:34,570
+و تلاتين من عشر تلاف الـ molarity للـ ascorbic acid
+
+315
+00:23:34,570 --> 00:23:41,160
+ثمانية و تلاتين من عشر تلاف 0.00038 ضرب الـ volume
+
+316
+00:23:41,160 --> 00:23:45,620
+للـ KIO3 الـ volume بتاع الـ KIO3 هذا بتاخده من ال
+
+317
+00:23:45,620 --> 00:23:49,120
+burette اللي هي وين وصلت الـ burette في أول معايرة
+
+318
+00:23:49,120 --> 00:23:55,380
+جداش وصلت بكتب مثلا طلع عندي 25 او خمسة ميل في أول
+
+319
+00:23:55,380 --> 00:23:59,480
+واحدة خمسة ميل فبقول تلاتة ضرب صفر point صفر صفر
+
+320
+00:23:59,480 --> 00:24:02,980
+تلاتة تمانية ضرب خمسة وبيطلع معايا الـ minimum لل
+
+321
+00:24:02,980 --> 00:24:07,580
+pure ascorbic acid بعدين ده حسب كمان مرة الملي مول
+
+322
+00:24:07,580 --> 00:24:12,860
+لـ natural ascorbic acid بقول تلاتة ضرب الـ molarity
+
+323
+00:24:12,860 --> 00:24:17,060
+للـ KIO3 ضرب الـ volume بتاع الـ KIO3 اللي هي تلاتة
+
+324
+00:24:17,060 --> 00:24:22,180
+ضرب 0.00038 ضرب الـ volume في حالة الـ natural لما
+
+325
+00:24:22,180 --> 00:24:26,660
+عملتي معايرة للـ natural لوين وصلت الـ pure عفوا مثلا
+
+326
+00:24:26,660 --> 00:24:31,020
+كان عندك تلاتة فتلاتة ميل فبقول تلاتة ضرب صفر
+
+327
+00:24:31,020 --> 00:24:36,020
+point 00308 ضرب تلاتة وبيطلع الجواب بعدين ده حسب
+
+328
+00:24:36,020 --> 00:24:40,180
+كمان مرة المول ملي مول للـ ascorbic acid في ال
+
+329
+00:24:40,180 --> 00:24:45,370
+artificial في الصناعي على فرض كانت اتنين ميل بقول 3
+
+330
+00:24:45,370 --> 00:24:52,030
+ضرب 0.000380 ضرب 2 وبيطلع الجواب هذه أول حسابات
+
+331
+00:24:52,030 --> 00:24:55,630
+أني بدي أحسب الـ millimole لـ pure ascorbic acid و
+
+332
+00:24:55,630 --> 00:25:00,970
+لـ الـ natural و لـ الـ artificial لو بدي أحسب ال
+
+333
+00:25:00,970 --> 00:25:04,630
+molarity إيش قانون الـ molarity الـ molarity بتساوي
+
+334
+00:25:04,630 --> 00:25:09,620
+الـ moles على الـ volume المولز على الـ volume بال
+
+335
+00:25:09,620 --> 00:25:14,040
+letter طيب المولز احنا عندنا ملي مول طلعت ملي مول
+
+336
+00:25:14,040 --> 00:25:16,960
+من القانون اللي قبل بكون مطلعة ملي مول و الـ volume
+
+337
+00:25:16,960 --> 00:25:22,060
+باخده بالـ milliliter إذا الـ molarity بتساوي المولز
+
+338
+00:25:22,060 --> 00:25:24,860
+على الـ volume لو بالـ letter عشان انا بتعامل ملي
+
+339
+00:25:24,860 --> 00:25:28,000
+مول فبدي احكي ملي مول على الـ volume بالـ milliliter
+
+340
+00:25:28,620 --> 00:25:31,820
+الـ milli مول هي اللي طلعتها من القانون اللي جابه لكل
+
+341
+00:25:31,820 --> 00:25:34,840
+واحدة الـ volume بالـ لتر اللي هو الـ volume
+
+342
+00:25:34,840 --> 00:25:37,940
+للـ ascorbic acid جداش أخدنا في أول واحدة خدنا من
+
+343
+00:25:37,940 --> 00:25:41,540
+الـ بيور 25 مل من الـ ascorbic acid في حالة الـ natural
+
+344
+00:25:41,540 --> 00:25:46,700
+برضه حطينا 25 مل من الـ natural juice في حالة ال
+
+345
+00:25:46,700 --> 00:25:51,180
+artificial برضه 25 مل من الـ artificial juice فهي
+
+346
+00:25:51,180 --> 00:25:55,520
+الـ volume ثابتة 25 مين هيتغير في الـ molarity؟ اللي
+
+347
+00:25:55,520 --> 00:26:00,100
+هو الـ millimoles اللي هي طلعتها من القانون اللي
+
+348
+00:26:00,100 --> 00:26:03,200
+جابه، جداش طلعتها للـ natural، جداش طلعتها للـ
+
+349
+00:26:03,200 --> 00:26:09,760
+artificial و جداش طلعتها للـ بيوت بعدين، أول حساب
+
+350
+00:26:09,760 --> 00:26:13,660
+حسبنا الـ millimoles ثاني حسب حسبنا الـ molarity
+
+351
+00:26:13,660 --> 00:26:17,700
+الـ milli مول حسبناها تلت مرات مرة للـ بيور مرة لل
+
+352
+00:26:17,700 --> 00:26:21,740
+natural مرة للـ artificial الـ molarity برضه بده
+
+353
+00:26:21,740 --> 00:26:25,980
+احسبها للـ بيور للـ natural و للـ artificial بعدين بده
+
+354
+00:26:25,980 --> 00:26:30,000
+احسب percentage of ascorbic acid in natural جداش
+
+355
+00:26:30,000 --> 00:26:34,600
+نسبة الـ ascorbic acid اللي موجودة في الجحيم الطبيعي
+
+356
+00:26:34,600 --> 00:26:39,180
+اللي بتساوي الـ molarity of natural على الـ molarity
+
+357
+00:26:39,180 --> 00:26:44,220
+of pure ascorbic acid اللي هي الـ molarity جداش طلعت
+
+358
+00:26:44,220 --> 00:26:47,160
+الـ molarity في الـ natural في الطبيعي على ال
+
+359
+00:26:47,160 --> 00:26:52,040
+molarity في الـ ascorbic acid الـ pure ضرب 100% هنا
+
+360
+00:26:52,040 --> 00:26:55,960
+مش مكتوب ضرب 100% إذا الـ percent للـ ascorbic acid
+
+361
+00:26:55,960 --> 00:26:59,320
+اللي في الـ natural بتساوي الـ molarity of natural
+
+362
+00:26:59,320 --> 00:27:03,160
+على الـ molarity of pure ضرب 100% طيب و لل
+
+363
+00:27:03,160 --> 00:27:06,520
+artificial percent للـ artificial molarity of
+
+364
+00:27:06,520 --> 00:27:12,530
+artificial على الـ molarity of pure ضرب 100% لو بدى
+
+365
+00:27:12,530 --> 00:27:15,910
+احسب الـ weight للـ Ascorbic Acid بتساوي المول ضرب
+
+366
+00:27:15,910 --> 00:27:18,350
+الـ molecular weight المول احنا حسبناه بنكون
+
+367
+00:27:18,350 --> 00:27:23,850
+حاسبينها الـ molecular weight 176.13 الـ molecular
+
+368
+00:27:23,850 --> 00:27:30,010
+weight للـ Ascorbic Acid 176.13 يمكن نحسبتها شوية
+
+369
+00:27:30,010 --> 00:27:35,110
+صعبة هنحكي عنها
\ No newline at end of file
diff --git a/PL9fwy3NUQKwZ1Q7zOg1ObgufPMjRI3WY1/vIoMDwX27cM_postprocess.srt b/PL9fwy3NUQKwZ1Q7zOg1ObgufPMjRI3WY1/vIoMDwX27cM_postprocess.srt
new file mode 100644
index 0000000000000000000000000000000000000000..24a5f5fdcc2895f224a785a44c3fb0cee11e460f
--- /dev/null
+++ b/PL9fwy3NUQKwZ1Q7zOg1ObgufPMjRI3WY1/vIoMDwX27cM_postprocess.srt
@@ -0,0 +1,1480 @@
+1
+00:00:00,000 --> 00:00:04,660
+العمل ليه لأ المعمل؟ المعمل بدنا نشتغل فيه
+
+2
+00:00:04,660 --> 00:00:08,220
+determination و vitamin C اللي هو ascorbic acid
+
+3
+00:00:08,220 --> 00:00:12,200
+concentration by redux titration with potassium
+
+4
+00:00:12,200 --> 00:00:18,290
+iodateبنحدد كمية vitamin C اللي هو الـ ascorbic
+
+5
+00:00:18,290 --> 00:00:24,210
+acid من خلال عملية المعايرة الـ titration بواسطة
+
+6
+00:00:24,210 --> 00:00:28,250
+بوتاسيوم ايودات ايش الـ titrant اللي بدي استخدمه
+
+7
+00:00:28,250 --> 00:00:32,470
+الـ titrant هو البوتاسيوم ايودات، لذا البوتاسيوم
+
+8
+00:00:32,470 --> 00:00:36,070
+ايودات هو عبارة عن الـ titrantفاليوم أنا بدي أجيب
+
+9
+00:00:36,070 --> 00:00:38,910
+pure ascorbic acid، ascorbic acid فيتامين،
+
+10
+00:00:38,910 --> 00:00:43,350
+ascorbic acid مش مضاعف عليه ولا أي شيء، هو pure، و
+
+11
+00:00:43,350 --> 00:00:46,750
+أعمله معايرة مع بوتاسيو مؤيدته، أشوف كده كميته،
+
+12
+00:00:46,750 --> 00:00:49,250
+أحسبله الـmolarity، و أحسبله الـmillimole، و
+
+13
+00:00:49,250 --> 00:00:55,830
+أحسبله الواتر، و بدي أعمل كمان مرة معايرة تانيةعلى
+
+14
+00:00:55,830 --> 00:00:59,770
+عصير برتقال طبيعي بدي أجيب عصير برتقال طب ليه؟
+
+15
+00:00:59,770 --> 00:01:03,030
+بيحتوي على vitamin C ال ascorbic acid بدي أشوف جدش
+
+16
+00:01:03,030 --> 00:01:07,990
+كمية ال ascorbic acid الموجودة فيه ومعايرة تالتة
+
+17
+00:01:07,990 --> 00:01:14,510
+بدي أجيب عصير برتقال صناعي عصير برتقال صناعي أشوف
+
+18
+00:01:14,510 --> 00:01:18,950
+كمية ال ascorbic acid الموجودة فيه vitamin C جدش
+
+19
+00:01:18,950 --> 00:01:24,700
+موجود فيهإذا ال objective to determine vitamin C
+
+20
+00:01:24,700 --> 00:01:28,420
+by potassium iodate titration أن أنا بدي أحدد كمية
+
+21
+00:01:28,420 --> 00:01:32,620
+vitamin C باستخدام potassium iodate titration وين
+
+22
+00:01:32,620 --> 00:01:38,500
+بدي أحدده في البيور ascorbic acid في البيور وين in
+
+23
+00:01:38,500 --> 00:01:42,640
+artificial orange juice وين in natural orange
+
+24
+00:01:42,640 --> 00:01:48,170
+juiceالـ principle أو خلّينا نحكي عن المعادل .. عن
+
+25
+00:01:48,170 --> 00:01:52,870
+ال .. عن التجربة ككل Vitamin C حكينا أن هو
+
+26
+00:01:52,870 --> 00:01:57,090
+antioxidant يعني reducing agent بيسموها خصة أي
+
+27
+00:01:57,090 --> 00:02:03,390
+تخزين يوائل الصيادلة باسم reducing agent لأن هو ضد
+
+28
+00:02:03,390 --> 00:02:08,590
+الأكسدا يعني عامل مختزن يعني بختزن غيره هو يصير له
+
+29
+00:02:08,590 --> 00:02:12,640
+أكسداSuitable method for the determination of
+
+30
+00:02:12,640 --> 00:02:15,420
+vitamin C quantities is titration with potassium
+
+31
+00:02:15,420 --> 00:02:20,320
+iodate بحكي لأفضل طريقة لتحديد كمية vitamin C عشان
+
+32
+00:02:20,320 --> 00:02:23,980
+أعرف كمية vitamin C هي أن يعمل عملية معايرة
+
+33
+00:02:23,980 --> 00:02:28,400
+titration مع ال potassium iodate potassium iodate
+
+34
+00:02:28,400 --> 00:02:32,850
+اللي هو ال KIO3 اللي بستخدمه كتيترانIodometric
+
+35
+00:02:32,850 --> 00:02:37,850
+titration أنه بتصير عملية معايرة في بداية المعايرة
+
+36
+00:02:37,850 --> 00:02:43,450
+الانالايت بيتفاعل بيصيرله reduce with excess
+
+37
+00:02:43,450 --> 00:02:50,210
+iodine I- ل I2 which turn blue in the presence of
+
+38
+00:02:50,210 --> 00:02:54,450
+starch بعدين ال I2 هاي بتتفاعل مع ال starch مع
+
+39
+00:02:54,450 --> 00:02:59,470
+النشط كإنديكاتور يعني وبيعطيني اللون، بيتغير اللون
+
+40
+00:03:01,270 --> 00:03:05,850
+عشان نفهم كل إشي بيصير فيها و نحكي هذا الكلام بدنا
+
+41
+00:03:05,850 --> 00:03:09,370
+نكون فاهمين إنه ال buret أنا بدي أعبيها بتايتران
+
+42
+00:03:09,370 --> 00:03:14,660
+إيش هو التايتران اللي بدي يكون موجود هو ال KIO3إذا
+
+43
+00:03:14,660 --> 00:03:18,660
+وظيفتي الـ KIO3 هو تيتران تمام هو البوتاسيوم
+
+44
+00:03:18,660 --> 00:03:21,920
+ايودات هذا بعبيه في البيور هجيب ال flask ال
+
+45
+00:03:21,920 --> 00:03:24,460
+volumetric flask اللي بدي أعمل فيها معايرة بدي أحط
+
+46
+00:03:24,460 --> 00:03:27,760
+فيها المادة اللي أنا بدي أعيرها بحط فيها كمية من
+
+47
+00:03:27,760 --> 00:03:31,080
+ال ascorbic acid اللي أنا بدي أعيره إذا بيور أو
+
+48
+00:03:31,080 --> 00:03:35,900
+إذا orange juice artificial أو naturalبدي أحط أول
+
+49
+00:03:35,900 --> 00:03:39,960
+مرة مثلا على البيورو بحط اسكوربيك أسد في ال flask
+
+50
+00:03:39,960 --> 00:03:45,300
+بنضيف عليها كمان potassium iodide potassium iodide
+
+51
+00:03:45,300 --> 00:03:49,760
+اللي هو ال KI potassium iodide هو ال KI بضيفه في
+
+52
+00:03:49,760 --> 00:03:53,660
+ال flask على ال ascorbic acid بيساهم في التفاعل و
+
+53
+00:03:53,660 --> 00:03:59,680
+عندنا هنشوف كده و بضيف كمية من HCl ليش ال HCl
+
+54
+00:03:59,680 --> 00:04:05,180
+بيعطيني ظروف حمضية للتفاعلو بضيف كمية من ال starch
+
+55
+00:04:05,180 --> 00:04:09,860
+اللى بيشتغل ك indicator اذا التفاعل مكوناته
+
+56
+00:04:09,860 --> 00:04:14,980
+potassium iodide iodate اللى هو ال KIO3 هذا لل
+
+57
+00:04:14,980 --> 00:04:19,560
+titrant المعينة وفى عندنا potassium iodide اللى هو
+
+58
+00:04:19,560 --> 00:04:23,920
+ال KI هذا بيسبب في التفاعل وفى عندنا HCL اللى
+
+59
+00:04:23,920 --> 00:04:28,270
+بيعطينا دروف حمضيةفيه ال starch بستخدمه ك
+
+60
+00:04:28,270 --> 00:04:31,950
+indicator و ال ascorbic acid اللي بتعمله معاهرة
+
+61
+00:04:31,950 --> 00:04:36,210
+عشان نفهم ال principle فجيت كيف اللي بدي اشتغل
+
+62
+00:04:36,210 --> 00:04:41,390
+التفاعل ال potassium iodate اللي هو ال KIO3 ركزوا
+
+63
+00:04:41,390 --> 00:04:47,550
+هان ال potassium iodate اللي هو ال KIO3 بيتفاعل مع
+
+64
+00:04:47,550 --> 00:04:55,170
+ال KI اللي هو ال potassium iodideKIO3 زائد KI زائد
+
+65
+00:04:55,170 --> 00:05:01,370
+potassium iodide زائد HCL في وجود دروف حمضية وسط
+
+66
+00:05:01,370 --> 00:05:08,180
+حمضية بيتكون عندى molecular iodine I2إذن أول ما
+
+67
+00:05:08,180 --> 00:05:11,260
+يبدأ التفاعل أبدأ أنزل من ال بيوريت ال potassium
+
+68
+00:05:11,260 --> 00:05:14,440
+iodate بفتح ال بيوريت و أنزل potassium iodate ال
+
+69
+00:05:14,440 --> 00:05:17,980
+titrant بيجي هذا ال potassium iodate ال KIO3
+
+70
+00:05:17,980 --> 00:05:21,640
+بيتفاعل مع ال KI مع ال potassium iodide اللي أنا
+
+71
+00:05:21,640 --> 00:05:26,000
+ضفتها في ال flask في وجود HCl دروف حمضية بيعطيني
+
+72
+00:05:26,000 --> 00:05:32,650
+molecular iodine I2الـ I2 هاي الـ Molecular Iodine
+
+73
+00:05:32,650 --> 00:05:37,490
+بس تتكون تتفاعل مع الـ Ascorbic Acid مع الاشي اللي
+
+74
+00:05:37,490 --> 00:05:40,010
+أنا بدي أعمله معايرة مع الـ Ascorbic Acid
+
+75
+00:05:40,770 --> 00:05:44,970
+الأسكوربيك أسد هو reducing agent يعني بيختز الغيره
+
+76
+00:05:44,970 --> 00:05:50,010
+لما يتفاعل مع ال أيودين بيعمل reducing ل I2
+
+77
+00:05:50,010 --> 00:05:57,110
+وبيحوله إلى reduce form اللي هو I- تمام؟ وهو
+
+78
+00:05:57,110 --> 00:06:02,650
+الأسكوربيك أسد بيصيرله أكسدة وبيتحول إلى ديهيدرو
+
+79
+00:06:02,650 --> 00:06:08,370
+أسكوربيك أسد أو بنسميه oxidized ascorbic acidإذن
+
+80
+00:06:08,370 --> 00:06:12,230
+أول ما يبدأ التفاعل بقى نزل من الـBurette titrant
+
+81
+00:06:12,230 --> 00:06:16,570
+اللي هو الـ Potassium Iodide الـ KIO3 بتتفاعل مع
+
+82
+00:06:16,570 --> 00:06:20,890
+Potassium Iodide اللي هي الـ KI في وجود HCl
+
+83
+00:06:20,890 --> 00:06:26,480
+بيعطيني Molecular Iodine I2بتيجي ال I2I بتتفعل مع
+
+84
+00:06:26,480 --> 00:06:31,160
+ال Ascorbic Acid بيصير عملية أكسدة و اختزاة ال I2
+
+85
+00:06:31,160 --> 00:06:36,080
+بتتحول ل I- و ال Ascorbic Acid بتتحول إلى Oxidized
+
+86
+00:06:36,080 --> 00:06:41,580
+Ascorbic Acid أو Dehydro Ascorbic Acidالجد بس
+
+87
+00:06:41,580 --> 00:06:45,640
+يتفاعل ال Iodine مع ال Ascorbic Acid بيضلوا يتفاعل
+
+88
+00:06:45,640 --> 00:06:48,440
+ال Iodine مع Ascorbic Acid ويحاولوا لديه Hydro
+
+89
+00:06:48,440 --> 00:06:52,500
+Ascorbic Acid ويظل يستمر التفاعل لحد ما يخلص ال
+
+90
+00:06:52,500 --> 00:06:57,980
+Ascorbic Acid مدى ما خلص ال Ascorbic Acid بيجي ال
+
+91
+00:06:57,980 --> 00:07:02,700
+Iodine هاد اللي ضايل بيتفاعل مع ال starch اللي
+
+92
+00:07:02,700 --> 00:07:09,360
+موجود عشان يعطيني لون permanent dark blue colorلون
+
+93
+00:07:09,360 --> 00:07:14,180
+أزرق غامق و يكون permanent هذا اللون يعني أنا بعمل
+
+94
+00:07:14,180 --> 00:07:19,000
+mix لل flask مايتغيرش اللون يظل مستمر اللون إذا
+
+95
+00:07:19,000 --> 00:07:22,940
+ضالوا مستمر اللون هاي بتكون ال end point عندها
+
+96
+00:07:22,940 --> 00:07:27,380
+خلاص وجف التفاعل خلص التفاعل بقرأ ال buret لوين
+
+97
+00:07:27,380 --> 00:07:31,940
+وصل حجمه و بحمل حسابات هنحكي عنها لبعدينإذن
+
+98
+00:07:31,940 --> 00:07:37,040
+التفاعل أول ما يبدأ بيتفعل KIO3 مع KI في وجود HCl
+
+99
+00:07:37,040 --> 00:07:41,400
+بيعطيني موليكولار Iodine I2 I2 بتتفاعل مع Ascorbic
+
+100
+00:07:41,400 --> 00:07:45,440
+Acid بيصير عملية أكسدة واختزال إلى Dehydro
+
+101
+00:07:45,440 --> 00:07:50,390
+Ascorbic Acid وI-هذا الجهاز يخلص كل الاسكوربيك اسن
+
+102
+00:07:50,390 --> 00:07:54,430
+الايضاين اللي ضايل ال I2 مليكولار ايضاين هاد
+
+103
+00:07:54,430 --> 00:07:59,530
+بيتفعل مع ال starch مع ال indicator وبيتغير اللون
+
+104
+00:07:59,530 --> 00:08:04,910
+وبيعطيني dark blue colorتمام و لازم يكون permanent
+
+105
+00:08:04,910 --> 00:08:10,010
+هذا اللون يعني لون دائم مستمر مش انه يكون مؤقت
+
+106
+00:08:10,010 --> 00:08:15,610
+ييجي و يختفي فعشان .. عشان ال iodine اضمن انه كمية
+
+107
+00:08:15,610 --> 00:08:20,870
+ال I2 بتكون كميات كبيرة بده احط ال KI في ال flask
+
+108
+00:08:20,870 --> 00:08:25,250
+البوتاسيوم iodide بده احط منه كميات زائدة in
+
+109
+00:08:25,250 --> 00:08:30,450
+excess amount فاحط ال KI ب excess amount بكميات
+
+110
+00:08:30,450 --> 00:08:34,900
+زائدةعشان أضمن استمراريتي التفاعل ويظل الـ KI
+
+111
+00:08:34,900 --> 00:08:39,480
+يتفاعل مع الـ KIO3 ويعطيني molecular Iodine و ال
+
+112
+00:08:39,480 --> 00:08:43,280
+Iodine يتفاعل مع ال Ascorbic Acid لعند ما يخلص و
+
+113
+00:08:43,280 --> 00:08:47,080
+يزيد من هذا ال Iodine يتفاعل مع ال star مش أنه ال
+
+114
+00:08:47,080 --> 00:08:51,200
+Iodine يكون كميته قليلةالـ Potassium Iodide يكون
+
+115
+00:08:51,200 --> 00:08:56,360
+كميته قليلة، مايعطنيش Iodine بكميات كفاية، هيخلص
+
+116
+00:08:56,360 --> 00:08:59,380
+ال Iodine قبل أن يخلص ال Ascorbic Acid، بالتالي
+
+117
+00:08:59,380 --> 00:09:02,400
+مافيش Iodine زيادة مش هيتفاعل مع الـ starch ولا
+
+118
+00:09:02,400 --> 00:09:06,990
+هيغير اللون، فبيحكي للـ Potassium Iodideالـ KI
+
+119
+00:09:06,990 --> 00:09:11,010
+بنحطه ب excess amount بكميات زيادة عشان أضمن
+
+120
+00:09:11,010 --> 00:09:15,630
+استمرارية التفاعل ويعطيني molecular iodine بكميات
+
+121
+00:09:15,630 --> 00:09:19,910
+زايدة بحيث انه يتفاعل مع ال ascorbic acid يظل
+
+122
+00:09:19,910 --> 00:09:24,230
+يتفاعل لحد ما يخلص ال ascorbic acid جابله و هو
+
+123
+00:09:24,230 --> 00:09:28,590
+بعدين يروح يتفاعل مع ال starch ويعطيني اللون ال
+
+124
+00:09:28,590 --> 00:09:34,180
+permanent dark blue colorهلجيت؟ كل slide هي محصلة
+
+125
+00:09:34,180 --> 00:09:38,240
+اللي حاكيناه إيش مكتوب؟ potassium iodate بنستخدمه
+
+126
+00:09:38,240 --> 00:09:42,360
+كtitrant اللي هو ال KIO3 بنستخدمه كtitrant بنضيفه
+
+127
+00:09:42,360 --> 00:09:46,320
+على ال Ascorbic acid وعلى strong acid على flask
+
+128
+00:09:46,320 --> 00:09:49,000
+هذا ال potassium iodate بيكون موجود في ال burette
+
+129
+00:09:49,000 --> 00:09:53,880
+بنزله على flask بتحتوي على Ascorbic acid وعلى
+
+130
+00:09:53,880 --> 00:09:58,570
+strong acid اللي هو ال HClو potassium iodide Ki
+
+131
+00:09:58,570 --> 00:10:02,330
+أجيت ال potassium iodate أول ما ينزل من ال buret
+
+132
+00:10:02,330 --> 00:10:06,170
+على ال flask بيتفاعل مع مين؟ بيتفاعل مع HCl و
+
+133
+00:10:06,170 --> 00:10:10,610
+potassium iodide بيتفاعل مع Ki في وجود HCl إيش
+
+134
+00:10:10,610 --> 00:10:14,850
+بيعطيني؟ ال molecular iodine ال I2 ال molecular
+
+135
+00:10:14,850 --> 00:10:19,030
+iodine هذا بيتفاعل مع ال ascorbic acid بيعمله
+
+136
+00:10:19,030 --> 00:10:24,000
+أكسدة بيكونليoxidized ascorbic acid اللي اسمه
+
+137
+00:10:24,000 --> 00:10:30,900
+dehydroascorbic acid اضافة الحمض بيحكيلي ليش فقط
+
+138
+00:10:30,900 --> 00:10:35,000
+ليه تعمل acidic condition بتعطيني ظروف حمضية إذا
+
+139
+00:10:35,000 --> 00:10:40,100
+ال acid وظيفته ظروف حمضية potassium iodide must be
+
+140
+00:10:40,100 --> 00:10:43,220
+added in excess to keep iodine dissolved بيحكيلي
+
+141
+00:10:43,220 --> 00:10:47,100
+ال KI اللي هي ال potassium iodide بنحطها بكميات
+
+142
+00:10:47,100 --> 00:10:52,550
+زائدةعشان اضمن انها تعطيني molecular iodine بكميات
+
+143
+00:10:52,550 --> 00:10:56,650
+كبيرة يدلوا يتفاعل مع ال ascorbic acid لعند ما
+
+144
+00:10:56,650 --> 00:11:01,150
+يخلص و لما يخلص يروح يتفاعل كمان هذا ال iodine
+
+145
+00:11:01,150 --> 00:11:06,470
+لزيادة مع ال starch و يعطيني ال end point once all
+
+146
+00:11:06,470 --> 00:11:09,410
+the ascorbic acid has been consumed بس يخلص ال
+
+147
+00:11:09,410 --> 00:11:13,690
+ascorbic acid ال excess من ال iodine اللي ضايل من
+
+148
+00:11:13,690 --> 00:11:18,630
+ال iodine من ال I2بيروح بيتفاعل مع ال starch
+
+149
+00:11:18,630 --> 00:11:22,530
+وبيعطيني ال end point بيحكي لي excess iodine قال
+
+150
+00:11:22,530 --> 00:11:26,490
+جت ال iod ايش لونه؟ لونه لون بني فال excess من ال
+
+151
+00:11:26,490 --> 00:11:29,350
+iodine اذا دلوا ال iodine بتفاعل مع كل ال ascorbic
+
+152
+00:11:29,350 --> 00:11:33,180
+acid وخلص ال ascorbic acidلون ال solution بيكون
+
+153
+00:11:33,180 --> 00:11:36,940
+لونه بني فممكن اعتمد على هذا اللون البني و احكي
+
+154
+00:11:36,940 --> 00:11:39,960
+انه خلاص مدام صار لون بني يعني ال iodine صار
+
+155
+00:11:39,960 --> 00:11:43,240
+بكميات زيادة يبجي هذه ال end point و ماضيفش ال
+
+156
+00:11:43,240 --> 00:11:47,060
+starch ان يكون هذا ال iodine ك it is on indicator
+
+157
+00:11:47,060 --> 00:11:49,880
+و انه هستخدمه ك indicator و خلاص و اواجه محطش
+
+158
+00:11:49,880 --> 00:11:54,400
+starch بيحكي لي هو بنفع انه يستخدمه ك indicator
+
+159
+00:11:54,400 --> 00:11:58,000
+لكن في هذه التجربة هنا difficult اني احدد ال end
+
+160
+00:11:58,000 --> 00:12:03,290
+point باستخدام ال iodine و اللونصعب علي ان احدد ال
+
+161
+00:12:03,290 --> 00:12:07,570
+end point باستخدام iodine لون ال iodine ليش؟ لان
+
+162
+00:12:07,570 --> 00:12:11,110
+احنا حاولنا ان نعمل عملية معايرة مع عصير برتقال
+
+163
+00:12:11,110 --> 00:12:15,350
+عصير البرتقال لونه برتقالي قريب من لون ال iodine
+
+164
+00:12:15,350 --> 00:12:20,020
+فممكن يصير عنديالتباس انه هل هذا من ال iodine اللي
+
+165
+00:12:20,020 --> 00:12:25,700
+زاد اللون ولا اللون اصلا عصير البرتقال فالافضل اني
+
+166
+00:12:25,700 --> 00:12:30,060
+بحكيلي بنضيف ال starch عشان يتفاعل مع ال iodine
+
+167
+00:12:30,060 --> 00:12:33,860
+وبيعطيني لون blue ال starch الناس بتتفاعل مع ال
+
+168
+00:12:33,860 --> 00:12:39,060
+iodine وبتعطيني لون blue لون أزرقوهذا اللون لازم
+
+169
+00:12:39,060 --> 00:12:42,440
+يكون permanent بيحكي لي the first permanent trace
+
+170
+00:12:42,440 --> 00:12:45,920
+of dark blue black color due to starch iodine
+
+171
+00:12:45,920 --> 00:12:49,300
+complex ماتما ادفع ال starch مع الايودين بيكونولي
+
+172
+00:12:49,300 --> 00:12:53,510
+complex لونه blue black color بيكون لونهاللون
+
+173
+00:12:53,510 --> 00:12:57,230
+الأزرق اللون الأزرق غامق اللون أزرق غامق اللون
+
+174
+00:12:57,230 --> 00:12:57,770
+أزرق غامق اللون أزرق غامق اللون أزرق غامق اللون
+
+175
+00:12:57,770 --> 00:13:00,610
+أزرق غامق اللون أزرق غامق اللون أزرق غامق اللون
+
+176
+00:13:00,610 --> 00:13:00,750
+أزرق غامق اللون أزرق غامق اللون أزرق غامق اللون
+
+177
+00:13:00,750 --> 00:13:03,750
+أزرق غامق اللون أزرق غامق اللون أزرق غامق اللون
+
+178
+00:13:03,750 --> 00:13:07,950
+أزرق غامق اللون أزرق غامق اللون أزرق غامق اللون
+
+179
+00:13:07,950 --> 00:13:11,750
+أزرق غامق اللون أزرق غامق اللون أزرق غامق اللون
+
+180
+00:13:11,750 --> 00:13:18,040
+أزرق غامق اللكيف بيختفي؟ انه بيكون نتيجة اتصاده من
+
+181
+00:13:18,040 --> 00:13:20,920
+ان انت لما نزلت وتكون عندك ال molecular iodine
+
+182
+00:13:20,920 --> 00:13:24,860
+اتصاده من ال I2 مع ال starch لكن سرعان ما يفك ال
+
+183
+00:13:24,860 --> 00:13:28,400
+iodine عن ال starch عشان فيه ascorbic acid بيفكوا
+
+184
+00:13:28,400 --> 00:13:31,240
+عن بعض و بيروح ال iodine بيتفعل مع ascorbic acid
+
+185
+00:13:31,940 --> 00:13:34,880
+انت لما تعملي mix هيفكوا مع بعض ويتفعل مع ascorbic
+
+186
+00:13:34,880 --> 00:13:38,200
+acid إذا خلص ال ascorbic acid إذا اتصادى من ال
+
+187
+00:13:38,200 --> 00:13:41,640
+iodine مع ال starch بيضل اللون مهما تعملي mix مش
+
+188
+00:13:41,640 --> 00:13:45,700
+هيفكوا مع بعض عن بعض لأنه مافيش ascorbic acid فانا
+
+189
+00:13:45,700 --> 00:13:48,900
+بدي ال permanent بدي اللون يكون permanent مش انه
+
+190
+00:13:48,900 --> 00:13:54,660
+يظهر و يختفيالـ Principle هي المعادلة اللي حكيناها
+
+191
+00:13:54,660 --> 00:14:00,060
+يعني الـ Principle هان بقدر ألخصها إنه KIO3 زائد
+
+192
+00:14:00,060 --> 00:14:05,340
+KI زائد HCl بيعطيني molecular iodine اللي هو الـI2
+
+193
+00:14:05,340 --> 00:14:10,020
+I2 زائد ascorbic acid بتتفهم من ال ascorbic acid؟
+
+194
+00:14:10,020 --> 00:14:15,070
+بيعطيني oxidized ascorbic acid زائد I minusهلجيت
+
+195
+00:14:15,070 --> 00:14:19,950
+ال excess من ال iodine ال I2 reaction with starch
+
+196
+00:14:19,950 --> 00:14:24,690
+and give permanent dark blue color هذه ملخص
+
+197
+00:14:24,690 --> 00:14:29,590
+التفاعل هذه المعادلة بتهمنا في حسابات لقدامها نحسب
+
+198
+00:14:29,590 --> 00:14:32,490
+احنا ال molarity و ال percentage و ال millimole و
+
+199
+00:14:32,490 --> 00:14:36,930
+ال watt كيف لو طلعنا على المعادلة انه مكتوب KIO3
+
+200
+00:14:36,930 --> 00:14:42,580
+مع KI مع H plus اللي بيعطيني تلاتة من ال I2إنه الـ
+
+201
+00:14:42,580 --> 00:14:49,340
+KIO3 كل واحد مول من الـ KIO3 بيعطيني تلاتة مول من
+
+202
+00:14:49,340 --> 00:14:54,800
+ال I2 من ال Iodine إذا كل واحد مول من ال KIO3
+
+203
+00:14:54,800 --> 00:14:59,340
+بيعطيني تلاتة مول من ال Iodine كل واحد Iodine من
+
+204
+00:14:59,340 --> 00:15:03,780
+هدول بيتفعل مع واحد Ascorbic Acid أنا بدي أصل
+
+205
+00:15:03,780 --> 00:15:10,260
+علاقة بين ال KIO3 و Ascorbic Acidبدي علاقة بين الـ
+
+206
+00:15:10,260 --> 00:15:14,540
+KIO3 و الـ ascorbic acid كيف بدي أجيب هذه العلاقة؟
+
+207
+00:15:14,540 --> 00:15:18,780
+كل واحد mol من الـ KIO3 بيعطيني تلاتة mol من ال
+
+208
+00:15:18,780 --> 00:15:22,220
+iodine بعدين كل واحد mol من ال iodine بيعطيني واحد
+
+209
+00:15:22,220 --> 00:15:25,320
+mol من ال ascorbic acid يعني التلاتة mol من ال
+
+210
+00:15:25,320 --> 00:15:30,970
+iodineمع تلاتة ascorbic acid مع واحد KIO3 تمام
+
+211
+00:15:30,970 --> 00:15:36,350
+فواحد مول من ال KIO3
+
+212
+00:15:36,350 --> 00:15:41,050
+بتعطيني تلاتة مول ascorbic acid هذه علاقة مهمة في
+
+213
+00:15:41,050 --> 00:15:47,530
+حساباتنا لجدام كل واحد مول من ال KIO3 يعطيني تلاتة
+
+214
+00:15:47,530 --> 00:15:53,100
+مول من ال ascorbic acidهنا الـ structure مش مطلوب
+
+215
+00:15:53,100 --> 00:15:56,880
+منها للاسكوربيك أسد ولا الديهيدرو أسكوربيك أسد
+
+216
+00:15:56,880 --> 00:16:02,900
+بيحكيلي هنا ملاحظة ال ink point بأوصلها لما يتحول
+
+217
+00:16:02,900 --> 00:16:08,080
+اللون ل permanent dark blue color مركز على كلمة
+
+218
+00:16:08,080 --> 00:16:11,500
+permanent ومن وين أجت هذا ال permanent dark blue
+
+219
+00:16:11,500 --> 00:16:14,540
+color؟ من وين أجه هذا اللون؟ بسبب التكوين complex
+
+220
+00:16:14,540 --> 00:16:18,890
+بين ال starch و الiodineDuring an iodometric
+
+221
+00:16:18,890 --> 00:16:21,870
+titration intermediate dark blue iodine starch
+
+222
+00:16:21,870 --> 00:16:25,930
+complex may form imminently before the iodine
+
+223
+00:16:25,930 --> 00:16:29,770
+react with ascorbic acid بيحكيلي أثناء المعايرة
+
+224
+00:16:29,770 --> 00:16:35,520
+ممكن يتكون عندك اللون ال dark blueبسبب اصطدام ال
+
+225
+00:16:35,520 --> 00:16:39,300
+iodine مع ال starch كونه complex لكن سرعان ما يفكه
+
+226
+00:16:39,300 --> 00:16:43,400
+و يروح ال iodine يتفاعل مع ascorbic acid لأنه لسه
+
+227
+00:16:43,400 --> 00:16:47,800
+موجود فلو اللون هذا اختفى disappear مع ال mixing
+
+228
+00:16:47,800 --> 00:16:51,580
+لما تعملي mix يختفي اذا هذي مش ال end point انا
+
+229
+00:16:51,580 --> 00:16:57,180
+بكونش وصلت لل end pointIodometric Titration
+
+230
+00:16:57,180 --> 00:17:02,320
+موليكولير Iodine بيحيل الموليكولير I2 لكن هذا
+
+231
+00:17:02,320 --> 00:17:06,700
+بيتفاعل مع ال Ascorbic Acid هو ضعيف الذائبية
+
+232
+00:17:06,700 --> 00:17:12,780
+ذائبيته ضعيفة slightly سوليومي فبيتفاعل مع ال KI
+
+233
+00:17:12,780 --> 00:17:17,760
+ال I2 هاي بس تنتج بتتفاعل هي حقيقة في التفاعل ال
+
+234
+00:17:17,760 --> 00:17:22,120
+I2 ذائبيتها ضعيفة صعب تتفاعل مع ال Ascorbic Acid
+
+235
+00:17:22,120 --> 00:17:26,750
+فماشي اللي بيصير في التفاعلتتحول الـ I2I بتتفاعل
+
+236
+00:17:26,750 --> 00:17:31,710
+مع الـ Potassium Iodide مع الـ Ki و بتعطي مركب
+
+237
+00:17:31,710 --> 00:17:38,450
+اسمه Triiodide I-3 هذا الـ Triiodide ذائب هو اللي
+
+238
+00:17:38,450 --> 00:17:42,890
+بيتفاعل مع الـ Ascorbic Acid حقيقة احنا حكينا عن
+
+239
+00:17:42,890 --> 00:17:46,310
+المعادلة ان المونيكولار I2I هو اللي بتفاعل مع الـ
+
+240
+00:17:46,310 --> 00:17:51,930
+Ascorbic Acid عشان بدناش نفرق لكن هو حقيقة الـ I2I
+
+241
+00:17:52,170 --> 00:17:57,350
+ذقبيتها ضعيفة تتفاعل مع الـ Ki و بتعطيني Triiodide
+
+242
+00:17:57,350 --> 00:18:01,630
+هو اللي بيتفاعل مع ال Ascorbic Acid ال starch is
+
+243
+00:18:01,630 --> 00:18:05,770
+used as indicator إيش وظيفة ال starch هان ك
+
+244
+00:18:05,770 --> 00:18:09,170
+indicator ككاشف بيعطيني اللون و بيعطيني ال end
+
+245
+00:18:09,170 --> 00:18:13,230
+point بيعمل complex مع ال iodine و بيعطيني اللون
+
+246
+00:18:15,100 --> 00:18:18,740
+طيب التجربة كيف خطوات إجراء التجربة؟ التجربة هذه
+
+247
+00:18:18,740 --> 00:18:23,040
+بدي أعمل عملية معايرة على تلت مرة أول مرة بدي
+
+248
+00:18:23,040 --> 00:18:26,660
+أعملها على pure ascorbic acid تاني مرة على عصير
+
+249
+00:18:26,660 --> 00:18:32,060
+برتقال طبيعي و تالت مرة على عصير برتقال صناعي هلجت
+
+250
+00:18:32,060 --> 00:18:34,700
+إن ال buret في التلت حالات ال buret إيش بدي
+
+251
+00:18:34,700 --> 00:18:37,300
+أعبيها؟ بدي أعبيها بال titrant اللي هو البوتاسيوم
+
+252
+00:18:37,300 --> 00:18:41,840
+ايودات فأنا بدي أخد ال buret و أعبيها للآخر ب KIO3
+
+253
+00:18:41,840 --> 00:18:46,770
+اللي هو بوتاسيوم ايوداتالـ Flask بدي أضع عليها في
+
+254
+00:18:46,770 --> 00:18:50,970
+أول واحد في ال pure ascorbic acid بدي أضع 25ml من
+
+255
+00:18:50,970 --> 00:18:57,310
+ال pure ascorbic acid على 4ml HCl على 3ml من ال
+
+256
+00:18:57,310 --> 00:19:03,490
+starch و 5ml من البوتاسيوم أيودانبنضع 25ml
+
+257
+00:19:03,490 --> 00:19:10,130
+Ascorbic Acid على 4ml HCl على 3ml Starch على 5ml
+
+258
+00:19:10,130 --> 00:19:17,050
+Ki وبعدين بفتح صمام لبيوريت أكيد استخدمته في
+
+259
+00:19:17,050 --> 00:19:22,740
+التحليلية في المعمل بفتح الصماممجرد مافتح الصمام
+
+260
+00:19:22,740 --> 00:19:26,780
+بيبدأ ينزل الـ KIO3 نقطة نقطة على الـ flask و هو
+
+261
+00:19:26,780 --> 00:19:30,880
+بينزل و أنا بعمل mix بنزل و بعمل mix متى ما اتكون
+
+262
+00:19:30,880 --> 00:19:35,000
+اللون اللي هو dark blue color و يكون permanent
+
+263
+00:19:35,000 --> 00:19:40,300
+اللون ثابت و بيختفيش بوقف المعايرة بسكر الصمام و
+
+264
+00:19:40,300 --> 00:19:44,680
+خلاص هنا انا وصلت لل end point بسجل قراعة البوريت
+
+265
+00:19:44,680 --> 00:19:48,840
+اللي هو ال volume بتاع ال KIO3 لوين وصلت البوريت
+
+266
+00:19:48,980 --> 00:19:53,480
+هذا ال volume من ال KIO3 أنا جداش استهلكت بسجل
+
+267
+00:19:53,480 --> 00:19:58,280
+عندي ال volume تمام بعد هيك بدي أعيد التجربة بدي
+
+268
+00:19:58,280 --> 00:20:02,160
+أكب ال flask و أعيد التجربة مرة تانية على natural
+
+269
+00:20:02,160 --> 00:20:07,800
+orange juice بدي أجيب برضه ال buret فيها KIO3 و ال
+
+270
+00:20:07,800 --> 00:20:12,140
+flask بدي أحط فيها 25 مل من ال ascorbic acid على 4
+
+271
+00:20:12,140 --> 00:20:18,450
+مل HCl3 من ال star و 5 من ال KI تمام و بفتح صمام
+
+272
+00:20:18,450 --> 00:20:22,810
+ال buret بيبدأ ينزل عندي من ال KIO3 على ال
+
+273
+00:20:22,810 --> 00:20:26,410
+solution على ال flask و بعمل mix بضلني اعمل mix
+
+274
+00:20:26,410 --> 00:20:29,470
+لحد ما يظهر عندي اللون ال permanent dark blue
+
+275
+00:20:29,470 --> 00:20:36,360
+colorو بسجل عندى خلاص وصل اللون بسكر الصمام و بقرأ
+
+276
+00:20:36,360 --> 00:20:40,340
+ال volume لل KIO3 وين وصل و بسجل عندى هذا ال
+
+277
+00:20:40,340 --> 00:20:45,280
+volume لل KIO3 فى حالة ال natural juice بعدين بعمل
+
+278
+00:20:45,280 --> 00:20:49,460
+كمان مرة نفس الخطوات بس بدل ال natural juice بحط
+
+279
+00:20:49,460 --> 00:20:54,090
+25 ml من ال artificial juiceوزي ما هم أربعة من ال
+
+280
+00:20:54,090 --> 00:20:59,370
+hcl تلاتة من ال starch خمسة من ال ki و بفتح الصمم
+
+281
+00:20:59,370 --> 00:21:03,610
+بعمل معاهرة مدى ما اتحول اللون ل bare من ال dark
+
+282
+00:21:03,610 --> 00:21:09,270
+blue color بسكر الصمم هاد و بسجل عندي النتيجة هسجل
+
+283
+00:21:09,270 --> 00:21:13,090
+النتيجة اللي زهرت عندي اللي هو ال volume اللي أنا
+
+284
+00:21:13,090 --> 00:21:21,280
+قرعته هاي مش مهمة مش مطلوبة منهاهو هاي ملخص
+
+285
+00:21:21,280 --> 00:21:25,200
+الفيتامينات إيش هي كم من ال vitamin عندنا هلجيت
+
+286
+00:21:25,200 --> 00:21:29,360
+احنا بعد هاي التجربة بعد ما نسجل ال نتائج بنسجل
+
+287
+00:21:29,360 --> 00:21:34,840
+قراة ال KIO3 بنبدأ بعملية حسابات بنحسب ال molarity
+
+288
+00:21:34,840 --> 00:21:40,220
+و بنحسب ال millimol في حالة artificial و في حالة
+
+289
+00:21:40,220 --> 00:21:44,320
+ال natural
+
+290
+00:21:44,320 --> 00:21:49,180
+و في حالة البيونمش عارف واضحين هال ولا لأ، يعني
+
+291
+00:21:49,180 --> 00:21:52,760
+كبرتهم، أنا كتبتهم، هلجيت احنا من المعادلة اللي
+
+292
+00:21:52,760 --> 00:21:56,780
+تاو حكينا عنها، المعادلة اللي تاو حكينا عنها،
+
+293
+00:21:56,780 --> 00:22:01,440
+حكينا إن 1 مول من الـ KIO3 بيتفعل مع 3 مول
+
+294
+00:22:01,440 --> 00:22:06,640
+Ascorbic Acid، 1 مول KIO3 يعطيني 3 مول Ascorbic
+
+295
+00:22:06,640 --> 00:22:10,030
+Acidلو بنحولها لـ millimol أخدته في التحليلية أنه
+
+296
+00:22:10,030 --> 00:22:15,250
+بتصير 1 على N millimol KiO3 1 على N millimol
+
+297
+00:22:15,250 --> 00:22:20,490
+ascorbic acid و N هي عدد المولاها هلجيت؟ 1 على N
+
+298
+00:22:20,490 --> 00:22:25,030
+millimol KiO3 اللي هي 1 على 1 1 على N من millimol
+
+299
+00:22:25,030 --> 00:22:30,100
+ascorbic acid هي 1 على 3الـ millimole ايش بتساوي
+
+300
+00:22:30,100 --> 00:22:34,120
+بتساوي ال molarity ضرب ال volume فبدي احول ال KIO3
+
+301
+00:22:34,120 --> 00:22:37,180
+بدل ما هي millimole بدي اكتب فيها molarity ضرب
+
+302
+00:22:37,180 --> 00:22:41,660
+volume من ال KIO3 بيساوي تلت ال millimole من ال
+
+303
+00:22:41,660 --> 00:22:45,540
+ascorbic acid انا بدي احسب ال millimole لل
+
+304
+00:22:45,540 --> 00:22:49,140
+ascorbic acid فبيطلع عندى من هذا القانون بس ننتج
+
+305
+00:22:49,140 --> 00:22:52,780
+ان ال millimole للascorbic acid ايش بتساوي تلاتة
+
+306
+00:22:52,780 --> 00:22:59,460
+ضرب ال molarity ضرب ال volume تمام تلاتةضرب الـ
+
+307
+00:22:59,460 --> 00:23:03,480
+molarity ضرب ال volume إذا أول قانون أول حساب ده
+
+308
+00:23:03,480 --> 00:23:09,000
+نحسبه هو ال millimol للأسكار بكهسين اللي هو قانونه
+
+309
+00:23:09,000 --> 00:23:12,320
+تلاتة في ال molarity في ال volume بدي أحسبه هذا
+
+310
+00:23:12,320 --> 00:23:17,260
+مرة للبيور و مرة لل natural و مرة لل artificial
+
+311
+00:23:17,260 --> 00:23:20,720
+إيش بدي أعوض ال millimol للأسكار بكهسين مثلا في
+
+312
+00:23:20,720 --> 00:23:24,270
+حالة البيور؟ إيش بدي أكتب؟ بتساويتلاتة ضرب ال
+
+313
+00:23:24,270 --> 00:23:30,210
+molarity ثابت ال molarity هي 0.00038 هي تمانية
+
+314
+00:23:30,210 --> 00:23:34,570
+وتلاتين من عشر تلاف ال molarity لل ascorbic acid
+
+315
+00:23:34,570 --> 00:23:41,160
+تمانية وتلاتين من عشر تلاف 0.00038دارب ال volume
+
+316
+00:23:41,160 --> 00:23:45,620
+لل KIO3 ال volume بتاع ال KIO3 هذا بتاخده من ال
+
+317
+00:23:45,620 --> 00:23:49,120
+burette اللي هي وين وصلت ال burette في أول معايرة
+
+318
+00:23:49,120 --> 00:23:55,380
+جدش وصلت بكتب مثلا طلع عندي 25 او خمسة ميل في أول
+
+319
+00:23:55,380 --> 00:23:59,480
+واحدة خمسة ميل فبقول تلاتة دارب سفر point سفر سفر
+
+320
+00:23:59,480 --> 00:24:02,980
+تلاتة تمانية دارب خمسة وبيطلع معايا ال minimum لل
+
+321
+00:24:02,980 --> 00:24:07,580
+pure ascorbic acidبعدين ده حسب كمان مرة الملي مول
+
+322
+00:24:07,580 --> 00:24:12,860
+لـnatural ascorbic acid بقول تلاتة ضرب الـmolarity
+
+323
+00:24:12,860 --> 00:24:17,060
+للـKIO3 ضرب ال volume بتاع الـKIO3 اللي هي تلاتة
+
+324
+00:24:17,060 --> 00:24:22,180
+ضرب 0.00038 ضرب ال volume في حالة ال natural لما
+
+325
+00:24:22,180 --> 00:24:26,660
+عملتي معايرة لل natural لوين وصلت ال pureعفرد مثلا
+
+326
+00:24:26,660 --> 00:24:31,020
+كان عندك تلاتة فتلاتة ميل فبقول تلاتة ضرب سفر
+
+327
+00:24:31,020 --> 00:24:36,020
+point 00308 ضرب تلاتة وبطلع الجواب بعدين ده حسب
+
+328
+00:24:36,020 --> 00:24:40,180
+كمان مرة المول ملي مول لل ascorbic acid في ال
+
+329
+00:24:40,180 --> 00:24:45,370
+artificial في الصناعي على فرض كانت اتنين ميلبقول 3
+
+330
+00:24:45,370 --> 00:24:52,030
+ضارب 0.000380 ضارب 2 وبطلّع الجواب هذه أول حسابات
+
+331
+00:24:52,030 --> 00:24:55,630
+أني بدي أحسب ال millimole لـ pure ascorbic acid و
+
+332
+00:24:55,630 --> 00:25:00,970
+ل ال natural و ل ال artificial لو بدي أحسب ال
+
+333
+00:25:00,970 --> 00:25:04,630
+molarity إيش قانون ال molarity ال molarity بتساوي
+
+334
+00:25:04,630 --> 00:25:09,620
+ال moles على ال volumeالمولز على ال volume بال
+
+335
+00:25:09,620 --> 00:25:14,040
+letter طيب المولز احنا عندنا ملي مول طلعت ملي مول
+
+336
+00:25:14,040 --> 00:25:16,960
+من القانون اللي قبل بكون مطلعة ملي مول وال volume
+
+337
+00:25:16,960 --> 00:25:22,060
+باخده بال milliliter اذا المولارتي بتساوي المولز
+
+338
+00:25:22,060 --> 00:25:24,860
+على ال volume لو بال letter عشان انا بتعامل ملي
+
+339
+00:25:24,860 --> 00:25:28,000
+مول فبدي احكي ملي مول على ال volume بال milliliter
+
+340
+00:25:28,620 --> 00:25:31,820
+الملي مول هي اللي طلعتها من القانون اللي جابه لكل
+
+341
+00:25:31,820 --> 00:25:34,840
+واحدة ال volume بال لتر اللي هو ال volume
+
+342
+00:25:34,840 --> 00:25:37,940
+للascorbic acid جدش أخدنا في أول واحدة خدنا من
+
+343
+00:25:37,940 --> 00:25:41,540
+البيور 25 مل من الascorbic acid في حالة ال natural
+
+344
+00:25:41,540 --> 00:25:46,700
+برضه حطينا 25 مل من ال natural juice في حالة ال
+
+345
+00:25:46,700 --> 00:25:51,180
+artificial برضه 25 مل من ال artificial juice فهي
+
+346
+00:25:51,180 --> 00:25:55,520
+ال volume سابت 25مين هيتغير في ال molarity؟ اللي
+
+347
+00:25:55,520 --> 00:26:00,100
+هو ال millimoles اللي هي طلعتها من القانون اللي
+
+348
+00:26:00,100 --> 00:26:03,200
+جابه، جدش طلعتها لل natural، جدش طلعتها لل
+
+349
+00:26:03,200 --> 00:26:09,760
+artificial و جدش طلعتها لل بيوت بعدين، اول حساب
+
+350
+00:26:09,760 --> 00:26:13,660
+حسبنا ال millimolesتانى حسب حسبنا الـ molarity
+
+351
+00:26:13,660 --> 00:26:17,700
+الملي مول حسبناها تلت مرات مرة للبيور مرة لل
+
+352
+00:26:17,700 --> 00:26:21,740
+natural مرة لل artificial ال molarity برضه بده
+
+353
+00:26:21,740 --> 00:26:25,980
+احسبها للبيور لل natural و لل artificial بعدين بده
+
+354
+00:26:25,980 --> 00:26:30,000
+احسب percentage of ascorbic acid in natural جداش
+
+355
+00:26:30,000 --> 00:26:34,600
+نسبة الascorbic acid اللى موجودة في الجحيم الطبيعى
+
+356
+00:26:34,600 --> 00:26:39,180
+اللى بتساوي ال molarity of natural على ال molarity
+
+357
+00:26:39,180 --> 00:26:44,220
+of pure ascorbic acidاللي هي ال molarity جدش طلعت
+
+358
+00:26:44,220 --> 00:26:47,160
+ال molarity في ال natural في الطبيعي على ال
+
+359
+00:26:47,160 --> 00:26:52,040
+molarity في ال ascorbic acid ال pure ضرب 100% هنا
+
+360
+00:26:52,040 --> 00:26:55,960
+مش مكتوب ضرب 100% إذا ال percent لل ascorbic acid
+
+361
+00:26:55,960 --> 00:26:59,320
+اللي في ال natural بتساوي ال molarity of natural
+
+362
+00:26:59,320 --> 00:27:03,160
+على ال molarity of pure ضرب 100% طيب و لل
+
+363
+00:27:03,160 --> 00:27:06,520
+artificial percent لل artificial molarity of
+
+364
+00:27:06,520 --> 00:27:12,530
+artificial على ال molarity of pure ضرب 100%لو بدى
+
+365
+00:27:12,530 --> 00:27:15,910
+احسب ال weight لل Ascorbic Acid بتساوي المول ضرب
+
+366
+00:27:15,910 --> 00:27:18,350
+ال molecular weight المول احنا حسبناه بنكون
+
+367
+00:27:18,350 --> 00:27:23,850
+حاسبينها ال molecular weight 176.13 ال molecular
+
+368
+00:27:23,850 --> 00:27:30,010
+weight لل Ascorbic Acid 176.13 يمكن نحسبتها شوية
+
+369
+00:27:30,010 --> 00:27:35,110
+صعبة هنحكي عنها في المعمل لما ناخد التجربة وهك
+
+370
+00:27:35,110 --> 00:27:36,970
+بيكون خلص هذا المعمل
+
diff --git a/PL9fwy3NUQKwZ1Q7zOg1ObgufPMjRI3WY1/vIoMDwX27cM_raw.json b/PL9fwy3NUQKwZ1Q7zOg1ObgufPMjRI3WY1/vIoMDwX27cM_raw.json
new file mode 100644
index 0000000000000000000000000000000000000000..fa2d2609d1c3c4dbc5bf4ca2d4ffea7ba654c6b2
--- /dev/null
+++ b/PL9fwy3NUQKwZ1Q7zOg1ObgufPMjRI3WY1/vIoMDwX27cM_raw.json
@@ -0,0 +1 @@
+{"segments": [{"id": 1, "seek": 1282, "start": 0.0, "end": 12.82, "text": "العمل ليه لأ المعمل؟ المعمل بدنا نشتغل فيه determination و vitamin C اللي هو ascorbic acid concentration by redux titration with potassium iodate", "tokens": [6027, 25957, 1211, 32239, 3224, 5296, 10721, 9673, 25957, 1211, 22807, 9673, 25957, 1211, 47525, 8315, 8717, 8592, 2655, 17082, 1211, 8978, 3224, 18432, 4032, 17163, 383, 13672, 1829, 31439, 15526, 30907, 299, 8258, 9856, 538, 2783, 87, 3459, 2405, 365, 29547, 44422, 473], "avg_logprob": -0.24288194047080147, "compression_ratio": 1.2808219178082192, "no_speech_prob": 1.0251998901367188e-05, "words": [{"start": 0.0, "end": 0.96, "word": "العمل", "probability": 0.59716796875}, {"start": 0.96, "end": 1.22, "word": " ليه", "probability": 0.771240234375}, {"start": 1.22, "end": 1.48, "word": " لأ", "probability": 0.815185546875}, {"start": 1.48, "end": 2.76, "word": " المعمل؟", "probability": 0.838134765625}, {"start": 2.76, "end": 3.36, "word": " المعمل", 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593.88, "word": " وعلى", "probability": 0.97216796875}, {"start": 593.88, "end": 594.38, "word": " strong", "probability": 0.95556640625}, {"start": 594.38, "end": 594.78, "word": " acid", "probability": 0.9599609375}, {"start": 594.78, "end": 594.96, "word": " اللي", "probability": 0.97607421875}, {"start": 594.96, "end": 595.08, "word": " هو", "probability": 0.98681640625}, {"start": 595.08, "end": 595.18, "word": " ال", "probability": 0.876953125}, {"start": 595.18, "end": 595.84, "word": " HCl", "probability": 0.7216796875}], "temperature": 1.0}, {"id": 29, "seek": 62045, "start": 596.01, "end": 620.45, "text": "و potassium iodide Ki أجيت ال potassium iodate أول ما ينزل من ال buret على ال flask بيتفاعل مع مين؟ بيتفاعل مع HCl و potassium iodide بيتفاعل مع Ki في وجود HCl إيش بيعطيني؟ ال molecular iodine ال I2 ال molecular iodine هذا بيتفاعل مع ال ascorbic acid بيعمله أكسدة بيكونلي", "tokens": [2407, 29547, 44422, 482, 17459, 5551, 7435, 36081, 2423, 29547, 44422, 473, 5551, 12610, 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ليش فقط ليه تعمل acidic condition بتعطيني ظروف حمضية إذا ال acid وظيفته ظروف حمضية potassium iodide must be added in excess to keep iodine dissolved بيحكيلي ال KI اللي هي ال potassium iodide بنحطها بكميات زائدة", "tokens": [5230, 327, 1602, 15526, 30907, 299, 8258, 13672, 1829, 24525, 2304, 3224, 32102, 340, 4806, 30907, 299, 8258, 1975, 11242, 31845, 3660, 21542, 2304, 11242, 4724, 1829, 5016, 4117, 1829, 20292, 32239, 8592, 6156, 47432, 32239, 3224, 6055, 25957, 1211, 39514, 4188, 39894, 3615, 9566, 9957, 1829, 1357, 116, 32887, 5172, 11331, 2304, 11242, 10632, 11933, 15730, 2423, 8258, 4032, 19913, 33911, 47395, 1357, 116, 32887, 5172, 11331, 2304, 11242, 10632, 29547, 44422, 482, 1633, 312, 3869, 294, 9310, 281, 1066, 44422, 533, 30651, 4724, 1829, 5016, 4117, 1829, 20292, 2423, 47261, 13672, 1829, 39896, 2423, 29547, 44422, 482, 44945, 5016, 9566, 11296, 4724, 24793, 1829, 9307, 30767, 16373, 41891], "avg_logprob": -0.15709459244667948, "compression_ratio": 1.6265560165975104, 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"word": " ظروف", "probability": 0.880126953125}, {"start": 636.54, "end": 637.08, "word": " حمضية", "probability": 0.997314453125}, {"start": 637.08, "end": 638.3, "word": " potassium", "probability": 0.94677734375}, {"start": 638.3, "end": 639.22, "word": " iodide", "probability": 0.96923828125}, {"start": 639.22, "end": 639.8, "word": " must", "probability": 0.859375}, {"start": 639.8, "end": 640.1, "word": " be", "probability": 0.9521484375}, {"start": 640.1, "end": 640.46, "word": " added", "probability": 0.8603515625}, {"start": 640.46, "end": 640.7, "word": " in", "probability": 0.9208984375}, {"start": 640.7, "end": 641.1, "word": " excess", "probability": 0.90283203125}, {"start": 641.1, "end": 641.34, "word": " to", "probability": 0.9794921875}, {"start": 641.34, "end": 641.56, "word": " keep", "probability": 0.939453125}, {"start": 641.56, "end": 641.98, "word": " iodine", "probability": 0.973876953125}, {"start": 641.98, "end": 642.4, "word": " dissolved", "probability": 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"probability": 0.80029296875}, {"start": 1633.37, "end": 1633.93, "word": " Ascorbic", "probability": 0.761444091796875}, {"start": 1633.93, "end": 1634.31, "word": " Acid", "probability": 0.793212890625}, {"start": 1634.31, "end": 1635.17, "word": " بتساوي", "probability": 0.8797607421875}, {"start": 1635.17, "end": 1635.61, "word": " المول", "probability": 0.78662109375}, {"start": 1635.61, "end": 1635.91, "word": " ضرب", "probability": 0.739990234375}, {"start": 1635.91, "end": 1636.07, "word": " ال", "probability": 0.91064453125}, {"start": 1636.07, "end": 1636.45, "word": " molecular", "probability": 0.90087890625}, {"start": 1636.45, "end": 1636.91, "word": " weight", "probability": 0.9521484375}, {"start": 1636.91, "end": 1637.33, "word": " المول", "probability": 0.89013671875}, {"start": 1637.33, "end": 1637.55, "word": " احنا", "probability": 0.784912109375}, {"start": 1637.55, "end": 1638.09, "word": " حسبناه", "probability": 0.892822265625}, {"start": 1638.09, "end": 1638.35, "word": " بنكون", "probability": 0.914794921875}, {"start": 1638.35, "end": 1639.15, "word": " حاسبينها", "probability": 0.9380859375}, {"start": 1639.15, "end": 1639.67, "word": " ال", "probability": 0.337158203125}, {"start": 1639.67, "end": 1640.01, "word": " molecular", "probability": 0.94091796875}, {"start": 1640.01, "end": 1640.49, "word": " weight", "probability": 0.96533203125}, {"start": 1640.49, "end": 1641.77, "word": " 176", "probability": 0.88134765625}, {"start": 1641.77, "end": 1642.81, "word": ".13", "probability": 0.97900390625}, {"start": 1642.81, "end": 1643.49, "word": " ال", "probability": 0.54443359375}, {"start": 1643.49, "end": 1643.85, "word": " molecular", "probability": 0.9140625}, {"start": 1643.85, "end": 1644.19, "word": " weight", "probability": 0.94140625}, {"start": 1644.19, "end": 1644.35, "word": " لل", "probability": 0.892578125}, {"start": 1644.35, "end": 1644.87, "word": " Ascorbic", "probability": 0.9632568359375}, {"start": 1644.87, "end": 1645.21, "word": " Acid", "probability": 0.966796875}, {"start": 1645.21, "end": 1646.73, "word": " 176", "probability": 0.96875}, {"start": 1646.73, "end": 1648.19, "word": ".13", "probability": 0.995849609375}, {"start": 1648.19, "end": 1649.01, "word": " يمكن", "probability": 0.974365234375}, {"start": 1649.01, "end": 1649.63, "word": " نحسبتها", "probability": 0.88115234375}, {"start": 1649.63, "end": 1650.01, "word": " شوية", "probability": 0.6778971354166666}, {"start": 1650.01, "end": 1650.45, "word": " صعبة", "probability": 0.9938151041666666}, {"start": 1650.45, "end": 1650.93, "word": " هنحكي", "probability": 0.910546875}, {"start": 1650.93, "end": 1651.29, "word": " عنها", "probability": 0.992919921875}, {"start": 1651.29, "end": 1651.83, "word": " في", "probability": 0.87255859375}, {"start": 1651.83, "end": 1652.59, "word": " المعمل", "probability": 0.85546875}, {"start": 1652.59, "end": 1652.83, "word": " لما", "probability": 0.951171875}, {"start": 1652.83, "end": 1653.53, "word": " ناخد", "probability": 0.9788411458333334}, {"start": 1653.53, "end": 1654.79, "word": " التجربة", "probability": 0.95556640625}, {"start": 1654.79, "end": 1655.11, "word": " وهك", "probability": 0.714599609375}, {"start": 1655.11, "end": 1655.49, "word": " بيكون", "probability": 0.8468424479166666}, {"start": 1655.49, "end": 1656.05, "word": " خلص", "probability": 0.9908854166666666}, {"start": 1656.05, "end": 1656.39, "word": " هذا", "probability": 0.50390625}, {"start": 1656.39, "end": 1656.97, "word": " المعمل", "probability": 0.9944661458333334}], "temperature": 1.0}], "language": "ar", "language_probability": 1.0, "duration": 1658.137, "duration_after_vad": 1627.2022187499967}
\ No newline at end of file
diff --git a/PL9fwy3NUQKwZ1Q7zOg1ObgufPMjRI3WY1/vIoMDwX27cM_raw.srt b/PL9fwy3NUQKwZ1Q7zOg1ObgufPMjRI3WY1/vIoMDwX27cM_raw.srt
new file mode 100644
index 0000000000000000000000000000000000000000..b52ddf660d18a467f3b92d20860b758331d3bd1b
--- /dev/null
+++ b/PL9fwy3NUQKwZ1Q7zOg1ObgufPMjRI3WY1/vIoMDwX27cM_raw.srt
@@ -0,0 +1,1484 @@
+1
+00:00:00,000 --> 00:00:04,660
+العمل ليه لأ المعمل؟ المعمل بدنا نشتغل فيه
+
+2
+00:00:04,660 --> 00:00:08,220
+determination و vitamin C اللي هو ascorbic acid
+
+3
+00:00:08,220 --> 00:00:12,200
+concentration by redux titration with potassium
+
+4
+00:00:12,200 --> 00:00:18,290
+iodateبنحدد كمية vitamin C اللي هو الـ ascorbic
+
+5
+00:00:18,290 --> 00:00:24,210
+acid من خلال عملية المعايرة الـ titration بواسطة
+
+6
+00:00:24,210 --> 00:00:28,250
+بوتاسيوم ايودات ايش الـ titrant اللي بدي استخدمه
+
+7
+00:00:28,250 --> 00:00:32,470
+الـ titrant هو البوتاسيوم ايودات، لذا البوتاسيوم
+
+8
+00:00:32,470 --> 00:00:36,070
+ايودات هو عبارة عن الـ titrantفاليوم أنا بدي أجيب
+
+9
+00:00:36,070 --> 00:00:38,910
+pure ascorbic acid، ascorbic acid فيتامين،
+
+10
+00:00:38,910 --> 00:00:43,350
+ascorbic acid مش مضاعف عليه ولا أي شيء، هو pure، و
+
+11
+00:00:43,350 --> 00:00:46,750
+أعمله معايرة مع بوتاسيو مؤيدته، أشوف كده كميته،
+
+12
+00:00:46,750 --> 00:00:49,250
+أحسبله الـmolarity، و أحسبله الـmillimole، و
+
+13
+00:00:49,250 --> 00:00:55,830
+أحسبله الواتر، و بدي أعمل كمان مرة معايرة تانيةعلى
+
+14
+00:00:55,830 --> 00:00:59,770
+عصير برتقال طبيعي بدي أجيب عصير برتقال طب ليه؟
+
+15
+00:00:59,770 --> 00:01:03,030
+بيحتوي على vitamin C ال ascorbic acid بدي أشوف جدش
+
+16
+00:01:03,030 --> 00:01:07,990
+كمية ال ascorbic acid الموجودة فيه ومعايرة تالتة
+
+17
+00:01:07,990 --> 00:01:14,510
+بدي أجيب عصير برتقال صناعي عصير برتقال صناعي أشوف
+
+18
+00:01:14,510 --> 00:01:18,950
+كمية ال ascorbic acid الموجودة فيه vitamin C جدش
+
+19
+00:01:18,950 --> 00:01:24,700
+موجود فيهإذا ال objective to determine vitamin C
+
+20
+00:01:24,700 --> 00:01:28,420
+by potassium iodate titration أن أنا بدي أحدد كمية
+
+21
+00:01:28,420 --> 00:01:32,620
+vitamin C باستخدام potassium iodate titration وين
+
+22
+00:01:32,620 --> 00:01:38,500
+بدي أحدده في البيور ascorbic acid في البيور وين in
+
+23
+00:01:38,500 --> 00:01:42,640
+artificial orange juice وين in natural orange
+
+24
+00:01:42,640 --> 00:01:48,170
+juiceالـ principle أو خلّينا نحكي عن المعادل .. عن
+
+25
+00:01:48,170 --> 00:01:52,870
+ال .. عن التجربة ككل Vitamin C حكينا أن هو
+
+26
+00:01:52,870 --> 00:01:57,090
+antioxidant يعني reducing agent بيسموها خصة أي
+
+27
+00:01:57,090 --> 00:02:03,390
+تخزين يوائل الصيادلة باسم reducing agent لأن هو ضد
+
+28
+00:02:03,390 --> 00:02:08,590
+الأكسدا يعني عامل مختزن يعني بختزن غيره هو يصير له
+
+29
+00:02:08,590 --> 00:02:12,640
+أكسداSuitable method for the determination of
+
+30
+00:02:12,640 --> 00:02:15,420
+vitamin C quantities is titration with potassium
+
+31
+00:02:15,420 --> 00:02:20,320
+iodate بحكي لأفضل طريقة لتحديد كمية vitamin C عشان
+
+32
+00:02:20,320 --> 00:02:23,980
+أعرف كمية vitamin C هي أن يعمل عملية معايرة
+
+33
+00:02:23,980 --> 00:02:28,400
+titration مع ال potassium iodate potassium iodate
+
+34
+00:02:28,400 --> 00:02:32,850
+اللي هو ال KIO3 اللي بستخدمه كتيترانIodometric
+
+35
+00:02:32,850 --> 00:02:37,850
+titration أنه بتصير عملية معايرة في بداية المعايرة
+
+36
+00:02:37,850 --> 00:02:43,450
+الانالايت بيتفاعل بيصيرله reduce with excess
+
+37
+00:02:43,450 --> 00:02:50,210
+iodine I- ل I2 which turn blue in the presence of
+
+38
+00:02:50,210 --> 00:02:54,450
+starch بعدين ال I2 هاي بتتفاعل مع ال starch مع
+
+39
+00:02:54,450 --> 00:02:59,470
+النشط كإنديكاتور يعني وبيعطيني اللون، بيتغير اللون
+
+40
+00:03:01,270 --> 00:03:05,850
+عشان نفهم كل إشي بيصير فيها و نحكي هذا الكلام بدنا
+
+41
+00:03:05,850 --> 00:03:09,370
+نكون فاهمين إنه ال buret أنا بدي أعبيها بتايتران
+
+42
+00:03:09,370 --> 00:03:14,660
+إيش هو التايتران اللي بدي يكون موجود هو ال KIO3إذا
+
+43
+00:03:14,660 --> 00:03:18,660
+وظيفتي الـ KIO3 هو تيتران تمام هو البوتاسيوم
+
+44
+00:03:18,660 --> 00:03:21,920
+ايودات هذا بعبيه في البيور هجيب ال flask ال
+
+45
+00:03:21,920 --> 00:03:24,460
+volumetric flask اللي بدي أعمل فيها معايرة بدي أحط
+
+46
+00:03:24,460 --> 00:03:27,760
+فيها المادة اللي أنا بدي أعيرها بحط فيها كمية من
+
+47
+00:03:27,760 --> 00:03:31,080
+ال ascorbic acid اللي أنا بدي أعيره إذا بيور أو
+
+48
+00:03:31,080 --> 00:03:35,900
+إذا orange juice artificial أو naturalبدي أحط أول
+
+49
+00:03:35,900 --> 00:03:39,960
+مرة مثلا على البيورو بحط اسكوربيك أسد في ال flask
+
+50
+00:03:39,960 --> 00:03:45,300
+بنضيف عليها كمان potassium iodide potassium iodide
+
+51
+00:03:45,300 --> 00:03:49,760
+اللي هو ال KI potassium iodide هو ال KI بضيفه في
+
+52
+00:03:49,760 --> 00:03:53,660
+ال flask على ال ascorbic acid بيساهم في التفاعل و
+
+53
+00:03:53,660 --> 00:03:59,680
+عندنا هنشوف كده و بضيف كمية من HCl ليش ال HCl
+
+54
+00:03:59,680 --> 00:04:05,180
+بيعطيني ظروف حمضية للتفاعلو بضيف كمية من ال starch
+
+55
+00:04:05,180 --> 00:04:09,860
+اللى بيشتغل ك indicator اذا التفاعل مكوناته
+
+56
+00:04:09,860 --> 00:04:14,980
+potassium iodide iodate اللى هو ال KIO3 هذا لل
+
+57
+00:04:14,980 --> 00:04:19,560
+titrant المعينة وفى عندنا potassium iodide اللى هو
+
+58
+00:04:19,560 --> 00:04:23,920
+ال KI هذا بيسبب في التفاعل وفى عندنا HCL اللى
+
+59
+00:04:23,920 --> 00:04:28,270
+بيعطينا دروف حمضيةفيه ال starch بستخدمه ك
+
+60
+00:04:28,270 --> 00:04:31,950
+indicator و ال ascorbic acid اللي بتعمله معاهرة
+
+61
+00:04:31,950 --> 00:04:36,210
+عشان نفهم ال principle فجيت كيف اللي بدي اشتغل
+
+62
+00:04:36,210 --> 00:04:41,390
+التفاعل ال potassium iodate اللي هو ال KIO3 ركزوا
+
+63
+00:04:41,390 --> 00:04:47,550
+هان ال potassium iodate اللي هو ال KIO3 بيتفاعل مع
+
+64
+00:04:47,550 --> 00:04:55,170
+ال KI اللي هو ال potassium iodideKIO3 زائد KI زائد
+
+65
+00:04:55,170 --> 00:05:01,370
+potassium iodide زائد HCL في وجود دروف حمضية وسط
+
+66
+00:05:01,370 --> 00:05:08,180
+حمضية بيتكون عندى molecular iodine I2إذن أول ما
+
+67
+00:05:08,180 --> 00:05:11,260
+يبدأ التفاعل أبدأ أنزل من ال بيوريت ال potassium
+
+68
+00:05:11,260 --> 00:05:14,440
+iodate بفتح ال بيوريت و أنزل potassium iodate ال
+
+69
+00:05:14,440 --> 00:05:17,980
+titrant بيجي هذا ال potassium iodate ال KIO3
+
+70
+00:05:17,980 --> 00:05:21,640
+بيتفاعل مع ال KI مع ال potassium iodide اللي أنا
+
+71
+00:05:21,640 --> 00:05:26,000
+ضفتها في ال flask في وجود HCl دروف حمضية بيعطيني
+
+72
+00:05:26,000 --> 00:05:32,650
+molecular iodine I2الـ I2 هاي الـ Molecular Iodine
+
+73
+00:05:32,650 --> 00:05:37,490
+بس تتكون تتفاعل مع الـ Ascorbic Acid مع الاشي اللي
+
+74
+00:05:37,490 --> 00:05:40,010
+أنا بدي أعمله معايرة مع الـ Ascorbic Acid
+
+75
+00:05:40,770 --> 00:05:44,970
+الأسكوربيك أسد هو reducing agent يعني بيختز الغيره
+
+76
+00:05:44,970 --> 00:05:50,010
+لما يتفاعل مع ال أيودين بيعمل reducing ل I2
+
+77
+00:05:50,010 --> 00:05:57,110
+وبيحوله إلى reduce form اللي هو I- تمام؟ وهو
+
+78
+00:05:57,110 --> 00:06:02,650
+الأسكوربيك أسد بيصيرله أكسدة وبيتحول إلى ديهيدرو
+
+79
+00:06:02,650 --> 00:06:08,370
+أسكوربيك أسد أو بنسميه oxidized ascorbic acidإذن
+
+80
+00:06:08,370 --> 00:06:12,230
+أول ما يبدأ التفاعل بقى نزل من الـBurette titrant
+
+81
+00:06:12,230 --> 00:06:16,570
+اللي هو الـ Potassium Iodide الـ KIO3 بتتفاعل مع
+
+82
+00:06:16,570 --> 00:06:20,890
+Potassium Iodide اللي هي الـ KI في وجود HCl
+
+83
+00:06:20,890 --> 00:06:26,480
+بيعطيني Molecular Iodine I2بتيجي ال I2I بتتفعل مع
+
+84
+00:06:26,480 --> 00:06:31,160
+ال Ascorbic Acid بيصير عملية أكسدة و اختزاة ال I2
+
+85
+00:06:31,160 --> 00:06:36,080
+بتتحول ل I- و ال Ascorbic Acid بتتحول إلى Oxidized
+
+86
+00:06:36,080 --> 00:06:41,580
+Ascorbic Acid أو Dehydro Ascorbic Acidالجد بس
+
+87
+00:06:41,580 --> 00:06:45,640
+يتفاعل ال Iodine مع ال Ascorbic Acid بيضلوا يتفاعل
+
+88
+00:06:45,640 --> 00:06:48,440
+ال Iodine مع Ascorbic Acid ويحاولوا لديه Hydro
+
+89
+00:06:48,440 --> 00:06:52,500
+Ascorbic Acid ويظل يستمر التفاعل لحد ما يخلص ال
+
+90
+00:06:52,500 --> 00:06:57,980
+Ascorbic Acid مدى ما خلص ال Ascorbic Acid بيجي ال
+
+91
+00:06:57,980 --> 00:07:02,700
+Iodine هاد اللي ضايل بيتفاعل مع ال starch اللي
+
+92
+00:07:02,700 --> 00:07:09,360
+موجود عشان يعطيني لون permanent dark blue colorلون
+
+93
+00:07:09,360 --> 00:07:14,180
+أزرق غامق و يكون permanent هذا اللون يعني أنا بعمل
+
+94
+00:07:14,180 --> 00:07:19,000
+mix لل flask مايتغيرش اللون يظل مستمر اللون إذا
+
+95
+00:07:19,000 --> 00:07:22,940
+ضالوا مستمر اللون هاي بتكون ال end point عندها
+
+96
+00:07:22,940 --> 00:07:27,380
+خلاص وجف التفاعل خلص التفاعل بقرأ ال buret لوين
+
+97
+00:07:27,380 --> 00:07:31,940
+وصل حجمه و بحمل حسابات هنحكي عنها لبعدينإذن
+
+98
+00:07:31,940 --> 00:07:37,040
+التفاعل أول ما يبدأ بيتفعل KIO3 مع KI في وجود HCl
+
+99
+00:07:37,040 --> 00:07:41,400
+بيعطيني موليكولار Iodine I2 I2 بتتفاعل مع Ascorbic
+
+100
+00:07:41,400 --> 00:07:45,440
+Acid بيصير عملية أكسدة واختزال إلى Dehydro
+
+101
+00:07:45,440 --> 00:07:50,390
+Ascorbic Acid وI-هذا الجهاز يخلص كل الاسكوربيك اسن
+
+102
+00:07:50,390 --> 00:07:54,430
+الايضاين اللي ضايل ال I2 مليكولار ايضاين هاد
+
+103
+00:07:54,430 --> 00:07:59,530
+بيتفعل مع ال starch مع ال indicator وبيتغير اللون
+
+104
+00:07:59,530 --> 00:08:04,910
+وبيعطيني dark blue colorتمام و لازم يكون permanent
+
+105
+00:08:04,910 --> 00:08:10,010
+هذا اللون يعني لون دائم مستمر مش انه يكون مؤقت
+
+106
+00:08:10,010 --> 00:08:15,610
+ييجي و يختفي فعشان .. عشان ال iodine اضمن انه كمية
+
+107
+00:08:15,610 --> 00:08:20,870
+ال I2 بتكون كميات كبيرة بده احط ال KI في ال flask
+
+108
+00:08:20,870 --> 00:08:25,250
+البوتاسيوم iodide بده احط منه كميات زائدة in
+
+109
+00:08:25,250 --> 00:08:30,450
+excess amount فاحط ال KI ب excess amount بكميات
+
+110
+00:08:30,450 --> 00:08:34,900
+زائدةعشان أضمن استمراريتي التفاعل ويظل الـ KI
+
+111
+00:08:34,900 --> 00:08:39,480
+يتفاعل مع الـ KIO3 ويعطيني molecular Iodine و ال
+
+112
+00:08:39,480 --> 00:08:43,280
+Iodine يتفاعل مع ال Ascorbic Acid لعند ما يخلص و
+
+113
+00:08:43,280 --> 00:08:47,080
+يزيد من هذا ال Iodine يتفاعل مع ال star مش أنه ال
+
+114
+00:08:47,080 --> 00:08:51,200
+Iodine يكون كميته قليلةالـ Potassium Iodide يكون
+
+115
+00:08:51,200 --> 00:08:56,360
+كميته قليلة، مايعطنيش Iodine بكميات كفاية، هيخلص
+
+116
+00:08:56,360 --> 00:08:59,380
+ال Iodine قبل أن يخلص ال Ascorbic Acid، بالتالي
+
+117
+00:08:59,380 --> 00:09:02,400
+مافيش Iodine زيادة مش هيتفاعل مع الـ starch ولا
+
+118
+00:09:02,400 --> 00:09:06,990
+هيغير اللون، فبيحكي للـ Potassium Iodideالـ KI
+
+119
+00:09:06,990 --> 00:09:11,010
+بنحطه ب excess amount بكميات زيادة عشان أضمن
+
+120
+00:09:11,010 --> 00:09:15,630
+استمرارية التفاعل ويعطيني molecular iodine بكميات
+
+121
+00:09:15,630 --> 00:09:19,910
+زايدة بحيث انه يتفاعل مع ال ascorbic acid يظل
+
+122
+00:09:19,910 --> 00:09:24,230
+يتفاعل لحد ما يخلص ال ascorbic acid جابله و هو
+
+123
+00:09:24,230 --> 00:09:28,590
+بعدين يروح يتفاعل مع ال starch ويعطيني اللون ال
+
+124
+00:09:28,590 --> 00:09:34,180
+permanent dark blue colorهلجيت؟ كل slide هي محصلة
+
+125
+00:09:34,180 --> 00:09:38,240
+اللي حاكيناه إيش مكتوب؟ potassium iodate بنستخدمه
+
+126
+00:09:38,240 --> 00:09:42,360
+كtitrant اللي هو ال KIO3 بنستخدمه كtitrant بنضيفه
+
+127
+00:09:42,360 --> 00:09:46,320
+على ال Ascorbic acid وعلى strong acid على flask
+
+128
+00:09:46,320 --> 00:09:49,000
+هذا ال potassium iodate بيكون موجود في ال burette
+
+129
+00:09:49,000 --> 00:09:53,880
+بنزله على flask بتحتوي على Ascorbic acid وعلى
+
+130
+00:09:53,880 --> 00:09:58,570
+strong acid اللي هو ال HClو potassium iodide Ki
+
+131
+00:09:58,570 --> 00:10:02,330
+أجيت ال potassium iodate أول ما ينزل من ال buret
+
+132
+00:10:02,330 --> 00:10:06,170
+على ال flask بيتفاعل مع مين؟ بيتفاعل مع HCl و
+
+133
+00:10:06,170 --> 00:10:10,610
+potassium iodide بيتفاعل مع Ki في وجود HCl إيش
+
+134
+00:10:10,610 --> 00:10:14,850
+بيعطيني؟ ال molecular iodine ال I2 ال molecular
+
+135
+00:10:14,850 --> 00:10:19,030
+iodine هذا بيتفاعل مع ال ascorbic acid بيعمله
+
+136
+00:10:19,030 --> 00:10:24,000
+أكسدة بيكونليoxidized ascorbic acid اللي اسمه
+
+137
+00:10:24,000 --> 00:10:30,900
+dehydroascorbic acid اضافة الحمض بيحكيلي ليش فقط
+
+138
+00:10:30,900 --> 00:10:35,000
+ليه تعمل acidic condition بتعطيني ظروف حمضية إذا
+
+139
+00:10:35,000 --> 00:10:40,100
+ال acid وظيفته ظروف حمضية potassium iodide must be
+
+140
+00:10:40,100 --> 00:10:43,220
+added in excess to keep iodine dissolved بيحكيلي
+
+141
+00:10:43,220 --> 00:10:47,100
+ال KI اللي هي ال potassium iodide بنحطها بكميات
+
+142
+00:10:47,100 --> 00:10:52,550
+زائدةعشان اضمن انها تعطيني molecular iodine بكميات
+
+143
+00:10:52,550 --> 00:10:56,650
+كبيرة يدلوا يتفاعل مع ال ascorbic acid لعند ما
+
+144
+00:10:56,650 --> 00:11:01,150
+يخلص و لما يخلص يروح يتفاعل كمان هذا ال iodine
+
+145
+00:11:01,150 --> 00:11:06,470
+لزيادة مع ال starch و يعطيني ال end point once all
+
+146
+00:11:06,470 --> 00:11:09,410
+the ascorbic acid has been consumed بس يخلص ال
+
+147
+00:11:09,410 --> 00:11:13,690
+ascorbic acid ال excess من ال iodine اللي ضايل من
+
+148
+00:11:13,690 --> 00:11:18,630
+ال iodine من ال I2بيروح بيتفاعل مع ال starch
+
+149
+00:11:18,630 --> 00:11:22,530
+وبيعطيني ال end point بيحكي لي excess iodine قال
+
+150
+00:11:22,530 --> 00:11:26,490
+جت ال iod ايش لونه؟ لونه لون بني فال excess من ال
+
+151
+00:11:26,490 --> 00:11:29,350
+iodine اذا دلوا ال iodine بتفاعل مع كل ال ascorbic
+
+152
+00:11:29,350 --> 00:11:33,180
+acid وخلص ال ascorbic acidلون ال solution بيكون
+
+153
+00:11:33,180 --> 00:11:36,940
+لونه بني فممكن اعتمد على هذا اللون البني و احكي
+
+154
+00:11:36,940 --> 00:11:39,960
+انه خلاص مدام صار لون بني يعني ال iodine صار
+
+155
+00:11:39,960 --> 00:11:43,240
+بكميات زيادة يبجي هذه ال end point و ماضيفش ال
+
+156
+00:11:43,240 --> 00:11:47,060
+starch ان يكون هذا ال iodine ك it is on indicator
+
+157
+00:11:47,060 --> 00:11:49,880
+و انه هستخدمه ك indicator و خلاص و اواجه محطش
+
+158
+00:11:49,880 --> 00:11:54,400
+starch بيحكي لي هو بنفع انه يستخدمه ك indicator
+
+159
+00:11:54,400 --> 00:11:58,000
+لكن في هذه التجربة هنا difficult اني احدد ال end
+
+160
+00:11:58,000 --> 00:12:03,290
+point باستخدام ال iodine و اللونصعب علي ان احدد ال
+
+161
+00:12:03,290 --> 00:12:07,570
+end point باستخدام iodine لون ال iodine ليش؟ لان
+
+162
+00:12:07,570 --> 00:12:11,110
+احنا حاولنا ان نعمل عملية معايرة مع عصير برتقال
+
+163
+00:12:11,110 --> 00:12:15,350
+عصير البرتقال لونه برتقالي قريب من لون ال iodine
+
+164
+00:12:15,350 --> 00:12:20,020
+فممكن يصير عنديالتباس انه هل هذا من ال iodine اللي
+
+165
+00:12:20,020 --> 00:12:25,700
+زاد اللون ولا اللون اصلا عصير البرتقال فالافضل اني
+
+166
+00:12:25,700 --> 00:12:30,060
+بحكيلي بنضيف ال starch عشان يتفاعل مع ال iodine
+
+167
+00:12:30,060 --> 00:12:33,860
+وبيعطيني لون blue ال starch الناس بتتفاعل مع ال
+
+168
+00:12:33,860 --> 00:12:39,060
+iodine وبتعطيني لون blue لون أزرقوهذا اللون لازم
+
+169
+00:12:39,060 --> 00:12:42,440
+يكون permanent بيحكي لي the first permanent trace
+
+170
+00:12:42,440 --> 00:12:45,920
+of dark blue black color due to starch iodine
+
+171
+00:12:45,920 --> 00:12:49,300
+complex ماتما ادفع ال starch مع الايودين بيكونولي
+
+172
+00:12:49,300 --> 00:12:53,510
+complex لونه blue black color بيكون لونهاللون
+
+173
+00:12:53,510 --> 00:12:57,230
+الأزرق اللون الأزرق غامق اللون أزرق غامق اللون
+
+174
+00:12:57,230 --> 00:12:57,770
+أزرق غامق اللون أزرق غامق اللون أزرق غامق اللون
+
+175
+00:12:57,770 --> 00:13:00,610
+أزرق غامق اللون أزرق غامق اللون أزرق غامق اللون
+
+176
+00:13:00,610 --> 00:13:00,610
+أزرق غامق اللون أزرق غامق اللون أزرق غامق اللون
+
+177
+00:13:00,610 --> 00:13:00,750
+أزرق غامق اللون أزرق غامق اللون أزرق غامق اللون
+
+178
+00:13:00,750 --> 00:13:03,750
+أزرق غامق اللون أزرق غامق اللون أزرق غامق اللون
+
+179
+00:13:03,750 --> 00:13:07,950
+أزرق غامق اللون أزرق غامق اللون أزرق غامق اللون
+
+180
+00:13:07,950 --> 00:13:11,750
+أزرق غامق اللون أزرق غامق اللون أزرق غامق اللون
+
+181
+00:13:11,750 --> 00:13:18,040
+أزرق غامق اللكيف بيختفي؟ انه بيكون نتيجة اتصاده من
+
+182
+00:13:18,040 --> 00:13:20,920
+ان انت لما نزلت وتكون عندك ال molecular iodine
+
+183
+00:13:20,920 --> 00:13:24,860
+اتصاده من ال I2 مع ال starch لكن سرعان ما يفك ال
+
+184
+00:13:24,860 --> 00:13:28,400
+iodine عن ال starch عشان فيه ascorbic acid بيفكوا
+
+185
+00:13:28,400 --> 00:13:31,240
+عن بعض و بيروح ال iodine بيتفعل مع ascorbic acid
+
+186
+00:13:31,940 --> 00:13:34,880
+انت لما تعملي mix هيفكوا مع بعض ويتفعل مع ascorbic
+
+187
+00:13:34,880 --> 00:13:38,200
+acid إذا خلص ال ascorbic acid إذا اتصادى من ال
+
+188
+00:13:38,200 --> 00:13:41,640
+iodine مع ال starch بيضل اللون مهما تعملي mix مش
+
+189
+00:13:41,640 --> 00:13:45,700
+هيفكوا مع بعض عن بعض لأنه مافيش ascorbic acid فانا
+
+190
+00:13:45,700 --> 00:13:48,900
+بدي ال permanent بدي اللون يكون permanent مش انه
+
+191
+00:13:48,900 --> 00:13:54,660
+يظهر و يختفيالـ Principle هي المعادلة اللي حكيناها
+
+192
+00:13:54,660 --> 00:14:00,060
+يعني الـ Principle هان بقدر ألخصها إنه KIO3 زائد
+
+193
+00:14:00,060 --> 00:14:05,340
+KI زائد HCl بيعطيني molecular iodine اللي هو الـI2
+
+194
+00:14:05,340 --> 00:14:10,020
+I2 زائد ascorbic acid بتتفهم من ال ascorbic acid؟
+
+195
+00:14:10,020 --> 00:14:15,070
+بيعطيني oxidized ascorbic acid زائد I minusهلجيت
+
+196
+00:14:15,070 --> 00:14:19,950
+ال excess من ال iodine ال I2 reaction with starch
+
+197
+00:14:19,950 --> 00:14:24,690
+and give permanent dark blue color هذه ملخص
+
+198
+00:14:24,690 --> 00:14:29,590
+التفاعل هذه المعادلة بتهمنا في حسابات لقدامها نحسب
+
+199
+00:14:29,590 --> 00:14:32,490
+احنا ال molarity و ال percentage و ال millimole و
+
+200
+00:14:32,490 --> 00:14:36,930
+ال watt كيف لو طلعنا على المعادلة انه مكتوب KIO3
+
+201
+00:14:36,930 --> 00:14:42,580
+مع KI مع H plus اللي بيعطيني تلاتة من ال I2إنه الـ
+
+202
+00:14:42,580 --> 00:14:49,340
+KIO3 كل واحد مول من الـ KIO3 بيعطيني تلاتة مول من
+
+203
+00:14:49,340 --> 00:14:54,800
+ال I2 من ال Iodine إذا كل واحد مول من ال KIO3
+
+204
+00:14:54,800 --> 00:14:59,340
+بيعطيني تلاتة مول من ال Iodine كل واحد Iodine من
+
+205
+00:14:59,340 --> 00:15:03,780
+هدول بيتفعل مع واحد Ascorbic Acid أنا بدي أصل
+
+206
+00:15:03,780 --> 00:15:10,260
+علاقة بين ال KIO3 و Ascorbic Acidبدي علاقة بين الـ
+
+207
+00:15:10,260 --> 00:15:14,540
+KIO3 و الـ ascorbic acid كيف بدي أجيب هذه العلاقة؟
+
+208
+00:15:14,540 --> 00:15:18,780
+كل واحد mol من الـ KIO3 بيعطيني تلاتة mol من ال
+
+209
+00:15:18,780 --> 00:15:22,220
+iodine بعدين كل واحد mol من ال iodine بيعطيني واحد
+
+210
+00:15:22,220 --> 00:15:25,320
+mol من ال ascorbic acid يعني التلاتة mol من ال
+
+211
+00:15:25,320 --> 00:15:30,970
+iodineمع تلاتة ascorbic acid مع واحد KIO3 تمام
+
+212
+00:15:30,970 --> 00:15:36,350
+فواحد مول من ال KIO3
+
+213
+00:15:36,350 --> 00:15:41,050
+بتعطيني تلاتة مول ascorbic acid هذه علاقة مهمة في
+
+214
+00:15:41,050 --> 00:15:47,530
+حساباتنا لجدام كل واحد مول من ال KIO3 يعطيني تلاتة
+
+215
+00:15:47,530 --> 00:15:53,100
+مول من ال ascorbic acidهنا الـ structure مش مطلوب
+
+216
+00:15:53,100 --> 00:15:56,880
+منها للاسكوربيك أسد ولا الديهيدرو أسكوربيك أسد
+
+217
+00:15:56,880 --> 00:16:02,900
+بيحكيلي هنا ملاحظة ال ink point بأوصلها لما يتحول
+
+218
+00:16:02,900 --> 00:16:08,080
+اللون ل permanent dark blue color مركز على كلمة
+
+219
+00:16:08,080 --> 00:16:11,500
+permanent ومن وين أجت هذا ال permanent dark blue
+
+220
+00:16:11,500 --> 00:16:14,540
+color؟ من وين أجه هذا اللون؟ بسبب التكوين complex
+
+221
+00:16:14,540 --> 00:16:18,890
+بين ال starch و الiodineDuring an iodometric
+
+222
+00:16:18,890 --> 00:16:21,870
+titration intermediate dark blue iodine starch
+
+223
+00:16:21,870 --> 00:16:25,930
+complex may form imminently before the iodine
+
+224
+00:16:25,930 --> 00:16:29,770
+react with ascorbic acid بيحكيلي أثناء المعايرة
+
+225
+00:16:29,770 --> 00:16:35,520
+ممكن يتكون عندك اللون ال dark blueبسبب اصطدام ال
+
+226
+00:16:35,520 --> 00:16:39,300
+iodine مع ال starch كونه complex لكن سرعان ما يفكه
+
+227
+00:16:39,300 --> 00:16:43,400
+و يروح ال iodine يتفاعل مع ascorbic acid لأنه لسه
+
+228
+00:16:43,400 --> 00:16:47,800
+موجود فلو اللون هذا اختفى disappear مع ال mixing
+
+229
+00:16:47,800 --> 00:16:51,580
+لما تعملي mix يختفي اذا هذي مش ال end point انا
+
+230
+00:16:51,580 --> 00:16:57,180
+بكونش وصلت لل end pointIodometric Titration
+
+231
+00:16:57,180 --> 00:17:02,320
+موليكولير Iodine بيحيل الموليكولير I2 لكن هذا
+
+232
+00:17:02,320 --> 00:17:06,700
+بيتفاعل مع ال Ascorbic Acid هو ضعيف الذائبية
+
+233
+00:17:06,700 --> 00:17:12,780
+ذائبيته ضعيفة slightly سوليومي فبيتفاعل مع ال KI
+
+234
+00:17:12,780 --> 00:17:17,760
+ال I2 هاي بس تنتج بتتفاعل هي حقيقة في التفاعل ال
+
+235
+00:17:17,760 --> 00:17:22,120
+I2 ذائبيتها ضعيفة صعب تتفاعل مع ال Ascorbic Acid
+
+236
+00:17:22,120 --> 00:17:26,750
+فماشي اللي بيصير في التفاعلتتحول الـ I2I بتتفاعل
+
+237
+00:17:26,750 --> 00:17:31,710
+مع الـ Potassium Iodide مع الـ Ki و بتعطي مركب
+
+238
+00:17:31,710 --> 00:17:38,450
+اسمه Triiodide I-3 هذا الـ Triiodide ذائب هو اللي
+
+239
+00:17:38,450 --> 00:17:42,890
+بيتفاعل مع الـ Ascorbic Acid حقيقة احنا حكينا عن
+
+240
+00:17:42,890 --> 00:17:46,310
+المعادلة ان المونيكولار I2I هو اللي بتفاعل مع الـ
+
+241
+00:17:46,310 --> 00:17:51,930
+Ascorbic Acid عشان بدناش نفرق لكن هو حقيقة الـ I2I
+
+242
+00:17:52,170 --> 00:17:57,350
+ذقبيتها ضعيفة تتفاعل مع الـ Ki و بتعطيني Triiodide
+
+243
+00:17:57,350 --> 00:18:01,630
+هو اللي بيتفاعل مع ال Ascorbic Acid ال starch is
+
+244
+00:18:01,630 --> 00:18:05,770
+used as indicator إيش وظيفة ال starch هان ك
+
+245
+00:18:05,770 --> 00:18:09,170
+indicator ككاشف بيعطيني اللون و بيعطيني ال end
+
+246
+00:18:09,170 --> 00:18:13,230
+point بيعمل complex مع ال iodine و بيعطيني اللون
+
+247
+00:18:15,100 --> 00:18:18,740
+طيب التجربة كيف خطوات إجراء التجربة؟ التجربة هذه
+
+248
+00:18:18,740 --> 00:18:23,040
+بدي أعمل عملية معايرة على تلت مرة أول مرة بدي
+
+249
+00:18:23,040 --> 00:18:26,660
+أعملها على pure ascorbic acid تاني مرة على عصير
+
+250
+00:18:26,660 --> 00:18:32,060
+برتقال طبيعي و تالت مرة على عصير برتقال صناعي هلجت
+
+251
+00:18:32,060 --> 00:18:34,700
+إن ال buret في التلت حالات ال buret إيش بدي
+
+252
+00:18:34,700 --> 00:18:37,300
+أعبيها؟ بدي أعبيها بال titrant اللي هو البوتاسيوم
+
+253
+00:18:37,300 --> 00:18:41,840
+ايودات فأنا بدي أخد ال buret و أعبيها للآخر ب KIO3
+
+254
+00:18:41,840 --> 00:18:46,770
+اللي هو بوتاسيوم ايوداتالـ Flask بدي أضع عليها في
+
+255
+00:18:46,770 --> 00:18:50,970
+أول واحد في ال pure ascorbic acid بدي أضع 25ml من
+
+256
+00:18:50,970 --> 00:18:57,310
+ال pure ascorbic acid على 4ml HCl على 3ml من ال
+
+257
+00:18:57,310 --> 00:19:03,490
+starch و 5ml من البوتاسيوم أيودانبنضع 25ml
+
+258
+00:19:03,490 --> 00:19:10,130
+Ascorbic Acid على 4ml HCl على 3ml Starch على 5ml
+
+259
+00:19:10,130 --> 00:19:17,050
+Ki وبعدين بفتح صمام لبيوريت أكيد استخدمته في
+
+260
+00:19:17,050 --> 00:19:22,740
+التحليلية في المعمل بفتح الصماممجرد مافتح الصمام
+
+261
+00:19:22,740 --> 00:19:26,780
+بيبدأ ينزل الـ KIO3 نقطة نقطة على الـ flask و هو
+
+262
+00:19:26,780 --> 00:19:30,880
+بينزل و أنا بعمل mix بنزل و بعمل mix متى ما اتكون
+
+263
+00:19:30,880 --> 00:19:35,000
+اللون اللي هو dark blue color و يكون permanent
+
+264
+00:19:35,000 --> 00:19:40,300
+اللون ثابت و بيختفيش بوقف المعايرة بسكر الصمام و
+
+265
+00:19:40,300 --> 00:19:44,680
+خلاص هنا انا وصلت لل end point بسجل قراعة البوريت
+
+266
+00:19:44,680 --> 00:19:48,840
+اللي هو ال volume بتاع ال KIO3 لوين وصلت البوريت
+
+267
+00:19:48,980 --> 00:19:53,480
+هذا ال volume من ال KIO3 أنا جداش استهلكت بسجل
+
+268
+00:19:53,480 --> 00:19:58,280
+عندي ال volume تمام بعد هيك بدي أعيد التجربة بدي
+
+269
+00:19:58,280 --> 00:20:02,160
+أكب ال flask و أعيد التجربة مرة تانية على natural
+
+270
+00:20:02,160 --> 00:20:07,800
+orange juice بدي أجيب برضه ال buret فيها KIO3 و ال
+
+271
+00:20:07,800 --> 00:20:12,140
+flask بدي أحط فيها 25 مل من ال ascorbic acid على 4
+
+272
+00:20:12,140 --> 00:20:18,450
+مل HCl3 من ال star و 5 من ال KI تمام و بفتح صمام
+
+273
+00:20:18,450 --> 00:20:22,810
+ال buret بيبدأ ينزل عندي من ال KIO3 على ال
+
+274
+00:20:22,810 --> 00:20:26,410
+solution على ال flask و بعمل mix بضلني اعمل mix
+
+275
+00:20:26,410 --> 00:20:29,470
+لحد ما يظهر عندي اللون ال permanent dark blue
+
+276
+00:20:29,470 --> 00:20:36,360
+colorو بسجل عندى خلاص وصل اللون بسكر الصمام و بقرأ
+
+277
+00:20:36,360 --> 00:20:40,340
+ال volume لل KIO3 وين وصل و بسجل عندى هذا ال
+
+278
+00:20:40,340 --> 00:20:45,280
+volume لل KIO3 فى حالة ال natural juice بعدين بعمل
+
+279
+00:20:45,280 --> 00:20:49,460
+كمان مرة نفس الخطوات بس بدل ال natural juice بحط
+
+280
+00:20:49,460 --> 00:20:54,090
+25 ml من ال artificial juiceوزي ما هم أربعة من ال
+
+281
+00:20:54,090 --> 00:20:59,370
+hcl تلاتة من ال starch خمسة من ال ki و بفتح الصمم
+
+282
+00:20:59,370 --> 00:21:03,610
+بعمل معاهرة مدى ما اتحول اللون ل bare من ال dark
+
+283
+00:21:03,610 --> 00:21:09,270
+blue color بسكر الصمم هاد و بسجل عندي النتيجة هسجل
+
+284
+00:21:09,270 --> 00:21:13,090
+النتيجة اللي زهرت عندي اللي هو ال volume اللي أنا
+
+285
+00:21:13,090 --> 00:21:21,280
+قرعته هاي مش مهمة مش مطلوبة منهاهو هاي ملخص
+
+286
+00:21:21,280 --> 00:21:25,200
+الفيتامينات إيش هي كم من ال vitamin عندنا هلجيت
+
+287
+00:21:25,200 --> 00:21:29,360
+احنا بعد هاي التجربة بعد ما نسجل ال نتائج بنسجل
+
+288
+00:21:29,360 --> 00:21:34,840
+قراة ال KIO3 بنبدأ بعملية حسابات بنحسب ال molarity
+
+289
+00:21:34,840 --> 00:21:40,220
+و بنحسب ال millimol في حالة artificial و في حالة
+
+290
+00:21:40,220 --> 00:21:44,320
+ال natural
+
+291
+00:21:44,320 --> 00:21:49,180
+و في حالة البيونمش عارف واضحين هال ولا لأ، يعني
+
+292
+00:21:49,180 --> 00:21:52,760
+كبرتهم، أنا كتبتهم، هلجيت احنا من المعادلة اللي
+
+293
+00:21:52,760 --> 00:21:56,780
+تاو حكينا عنها، المعادلة اللي تاو حكينا عنها،
+
+294
+00:21:56,780 --> 00:22:01,440
+حكينا إن 1 مول من الـ KIO3 بيتفعل مع 3 مول
+
+295
+00:22:01,440 --> 00:22:06,640
+Ascorbic Acid، 1 مول KIO3 يعطيني 3 مول Ascorbic
+
+296
+00:22:06,640 --> 00:22:10,030
+Acidلو بنحولها لـ millimol أخدته في التحليلية أنه
+
+297
+00:22:10,030 --> 00:22:15,250
+بتصير 1 على N millimol KiO3 1 على N millimol
+
+298
+00:22:15,250 --> 00:22:20,490
+ascorbic acid و N هي عدد المولاها هلجيت؟ 1 على N
+
+299
+00:22:20,490 --> 00:22:25,030
+millimol KiO3 اللي هي 1 على 1 1 على N من millimol
+
+300
+00:22:25,030 --> 00:22:30,100
+ascorbic acid هي 1 على 3الـ millimole ايش بتساوي
+
+301
+00:22:30,100 --> 00:22:34,120
+بتساوي ال molarity ضرب ال volume فبدي احول ال KIO3
+
+302
+00:22:34,120 --> 00:22:37,180
+بدل ما هي millimole بدي اكتب فيها molarity ضرب
+
+303
+00:22:37,180 --> 00:22:41,660
+volume من ال KIO3 بيساوي تلت ال millimole من ال
+
+304
+00:22:41,660 --> 00:22:45,540
+ascorbic acid انا بدي احسب ال millimole لل
+
+305
+00:22:45,540 --> 00:22:49,140
+ascorbic acid فبيطلع عندى من هذا القانون بس ننتج
+
+306
+00:22:49,140 --> 00:22:52,780
+ان ال millimole للascorbic acid ايش بتساوي تلاتة
+
+307
+00:22:52,780 --> 00:22:59,460
+ضرب ال molarity ضرب ال volume تمام تلاتةضرب الـ
+
+308
+00:22:59,460 --> 00:23:03,480
+molarity ضرب ال volume إذا أول قانون أول حساب ده
+
+309
+00:23:03,480 --> 00:23:09,000
+نحسبه هو ال millimol للأسكار بكهسين اللي هو قانونه
+
+310
+00:23:09,000 --> 00:23:12,320
+تلاتة في ال molarity في ال volume بدي أحسبه هذا
+
+311
+00:23:12,320 --> 00:23:17,260
+مرة للبيور و مرة لل natural و مرة لل artificial
+
+312
+00:23:17,260 --> 00:23:20,720
+إيش بدي أعوض ال millimol للأسكار بكهسين مثلا في
+
+313
+00:23:20,720 --> 00:23:24,270
+حالة البيور؟ إيش بدي أكتب؟ بتساويتلاتة ضرب ال
+
+314
+00:23:24,270 --> 00:23:30,210
+molarity ثابت ال molarity هي 0.00038 هي تمانية
+
+315
+00:23:30,210 --> 00:23:34,570
+وتلاتين من عشر تلاف ال molarity لل ascorbic acid
+
+316
+00:23:34,570 --> 00:23:41,160
+تمانية وتلاتين من عشر تلاف 0.00038دارب ال volume
+
+317
+00:23:41,160 --> 00:23:45,620
+لل KIO3 ال volume بتاع ال KIO3 هذا بتاخده من ال
+
+318
+00:23:45,620 --> 00:23:49,120
+burette اللي هي وين وصلت ال burette في أول معايرة
+
+319
+00:23:49,120 --> 00:23:55,380
+جدش وصلت بكتب مثلا طلع عندي 25 او خمسة ميل في أول
+
+320
+00:23:55,380 --> 00:23:59,480
+واحدة خمسة ميل فبقول تلاتة دارب سفر point سفر سفر
+
+321
+00:23:59,480 --> 00:24:02,980
+تلاتة تمانية دارب خمسة وبيطلع معايا ال minimum لل
+
+322
+00:24:02,980 --> 00:24:07,580
+pure ascorbic acidبعدين ده حسب كمان مرة الملي مول
+
+323
+00:24:07,580 --> 00:24:12,860
+لـnatural ascorbic acid بقول تلاتة ضرب الـmolarity
+
+324
+00:24:12,860 --> 00:24:17,060
+للـKIO3 ضرب ال volume بتاع الـKIO3 اللي هي تلاتة
+
+325
+00:24:17,060 --> 00:24:22,180
+ضرب 0.00038 ضرب ال volume في حالة ال natural لما
+
+326
+00:24:22,180 --> 00:24:26,660
+عملتي معايرة لل natural لوين وصلت ال pureعفرد مثلا
+
+327
+00:24:26,660 --> 00:24:31,020
+كان عندك تلاتة فتلاتة ميل فبقول تلاتة ضرب سفر
+
+328
+00:24:31,020 --> 00:24:36,020
+point 00308 ضرب تلاتة وبطلع الجواب بعدين ده حسب
+
+329
+00:24:36,020 --> 00:24:40,180
+كمان مرة المول ملي مول لل ascorbic acid في ال
+
+330
+00:24:40,180 --> 00:24:45,370
+artificial في الصناعي على فرض كانت اتنين ميلبقول 3
+
+331
+00:24:45,370 --> 00:24:52,030
+ضارب 0.000380 ضارب 2 وبطلّع الجواب هذه أول حسابات
+
+332
+00:24:52,030 --> 00:24:55,630
+أني بدي أحسب ال millimole لـ pure ascorbic acid و
+
+333
+00:24:55,630 --> 00:25:00,970
+ل ال natural و ل ال artificial لو بدي أحسب ال
+
+334
+00:25:00,970 --> 00:25:04,630
+molarity إيش قانون ال molarity ال molarity بتساوي
+
+335
+00:25:04,630 --> 00:25:09,620
+ال moles على ال volumeالمولز على ال volume بال
+
+336
+00:25:09,620 --> 00:25:14,040
+letter طيب المولز احنا عندنا ملي مول طلعت ملي مول
+
+337
+00:25:14,040 --> 00:25:16,960
+من القانون اللي قبل بكون مطلعة ملي مول وال volume
+
+338
+00:25:16,960 --> 00:25:22,060
+باخده بال milliliter اذا المولارتي بتساوي المولز
+
+339
+00:25:22,060 --> 00:25:24,860
+على ال volume لو بال letter عشان انا بتعامل ملي
+
+340
+00:25:24,860 --> 00:25:28,000
+مول فبدي احكي ملي مول على ال volume بال milliliter
+
+341
+00:25:28,620 --> 00:25:31,820
+الملي مول هي اللي طلعتها من القانون اللي جابه لكل
+
+342
+00:25:31,820 --> 00:25:34,840
+واحدة ال volume بال لتر اللي هو ال volume
+
+343
+00:25:34,840 --> 00:25:37,940
+للascorbic acid جدش أخدنا في أول واحدة خدنا من
+
+344
+00:25:37,940 --> 00:25:41,540
+البيور 25 مل من الascorbic acid في حالة ال natural
+
+345
+00:25:41,540 --> 00:25:46,700
+برضه حطينا 25 مل من ال natural juice في حالة ال
+
+346
+00:25:46,700 --> 00:25:51,180
+artificial برضه 25 مل من ال artificial juice فهي
+
+347
+00:25:51,180 --> 00:25:55,520
+ال volume سابت 25مين هيتغير في ال molarity؟ اللي
+
+348
+00:25:55,520 --> 00:26:00,100
+هو ال millimoles اللي هي طلعتها من القانون اللي
+
+349
+00:26:00,100 --> 00:26:03,200
+جابه، جدش طلعتها لل natural، جدش طلعتها لل
+
+350
+00:26:03,200 --> 00:26:09,760
+artificial و جدش طلعتها لل بيوت بعدين، اول حساب
+
+351
+00:26:09,760 --> 00:26:13,660
+حسبنا ال millimolesتانى حسب حسبنا الـ molarity
+
+352
+00:26:13,660 --> 00:26:17,700
+الملي مول حسبناها تلت مرات مرة للبيور مرة لل
+
+353
+00:26:17,700 --> 00:26:21,740
+natural مرة لل artificial ال molarity برضه بده
+
+354
+00:26:21,740 --> 00:26:25,980
+احسبها للبيور لل natural و لل artificial بعدين بده
+
+355
+00:26:25,980 --> 00:26:30,000
+احسب percentage of ascorbic acid in natural جداش
+
+356
+00:26:30,000 --> 00:26:34,600
+نسبة الascorbic acid اللى موجودة في الجحيم الطبيعى
+
+357
+00:26:34,600 --> 00:26:39,180
+اللى بتساوي ال molarity of natural على ال molarity
+
+358
+00:26:39,180 --> 00:26:44,220
+of pure ascorbic acidاللي هي ال molarity جدش طلعت
+
+359
+00:26:44,220 --> 00:26:47,160
+ال molarity في ال natural في الطبيعي على ال
+
+360
+00:26:47,160 --> 00:26:52,040
+molarity في ال ascorbic acid ال pure ضرب 100% هنا
+
+361
+00:26:52,040 --> 00:26:55,960
+مش مكتوب ضرب 100% إذا ال percent لل ascorbic acid
+
+362
+00:26:55,960 --> 00:26:59,320
+اللي في ال natural بتساوي ال molarity of natural
+
+363
+00:26:59,320 --> 00:27:03,160
+على ال molarity of pure ضرب 100% طيب و لل
+
+364
+00:27:03,160 --> 00:27:06,520
+artificial percent لل artificial molarity of
+
+365
+00:27:06,520 --> 00:27:12,530
+artificial على ال molarity of pure ضرب 100%لو بدى
+
+366
+00:27:12,530 --> 00:27:15,910
+احسب ال weight لل Ascorbic Acid بتساوي المول ضرب
+
+367
+00:27:15,910 --> 00:27:18,350
+ال molecular weight المول احنا حسبناه بنكون
+
+368
+00:27:18,350 --> 00:27:23,850
+حاسبينها ال molecular weight 176.13 ال molecular
+
+369
+00:27:23,850 --> 00:27:30,010
+weight لل Ascorbic Acid 176.13 يمكن نحسبتها شوية
+
+370
+00:27:30,010 --> 00:27:35,110
+صعبة هنحكي عنها في المعمل لما ناخد التجربة وهك
+
+371
+00:27:35,110 --> 00:27:36,970
+بيكون خلص هذا المعمل
+
diff --git a/PL9fwy3NUQKwZ1Q7zOg1ObgufPMjRI3WY1/vUaw7lH8dOs.srt b/PL9fwy3NUQKwZ1Q7zOg1ObgufPMjRI3WY1/vUaw7lH8dOs.srt
new file mode 100644
index 0000000000000000000000000000000000000000..d627414d5f1a10eff8d3f78ce958e7063ff3bfbe
--- /dev/null
+++ b/PL9fwy3NUQKwZ1Q7zOg1ObgufPMjRI3WY1/vUaw7lH8dOs.srt
@@ -0,0 +1,702 @@
+1
+00:00:01,150 --> 00:00:05,090
+اليوم إن شاء الله راح نحكي في أول معمل من معامل
+
+2
+00:00:05,090 --> 00:00:09,690
+الكيميا الحيوية هيكون عبارة عن مقدمة عامة عن 
+
+3
+00:00:09,690 --> 00:00:14,390
+المادة تعريف الكيميا الحيوية الأهداف العامة من 
+
+4
+00:00:14,390 --> 00:00:20,150
+دراسة المقرر وليش احنا بندرسه هنتعرف فيه على ال
+
+5
+00:00:20,150 --> 00:00:23,910
+safety rules داخل المعمل قواعد السلامة والأمان داخل
+
+6
+00:00:23,910 --> 00:00:28,220
+المعمل هنتعرف فيه على بعض الأدوات اللي هنستخدمها في 
+
+7
+00:00:28,220 --> 00:00:34,100
+المعمل و هندخل في مقدمة بسيطة عن الأحماض الأمينية 
+
+8
+00:00:34,100 --> 00:00:40,520
+في البداية خلينا نتعرف على تعريف الـ biochemistry
+
+9
+00:00:40,520 --> 00:00:44,930
+اللي هي الكيميا الحيوية لما نحكي كيميا حيوية، إيش 
+
+10
+00:00:44,930 --> 00:00:49,450
+بنستنتج منها؟ كيميا، كلمة كيميا معناها تفاعلات 
+
+11
+00:00:49,450 --> 00:00:54,270
+كيميائية بدها تكون حيوية يعني تحدث في داخل جسم 
+
+12
+00:00:54,270 --> 00:00:58,950
+الإنسان أو جسم الكائن الحي فهي تفاعلات كيميائية 
+
+13
+00:00:58,950 --> 00:01:03,820
+تحدث في داخل أجسام الكائنات الحية مش مكتوب هنا
+
+14
+00:01:03,820 --> 00:01:07,480
+مكتوب إن الـ biochemistry is the application of 
+
+15
+00:01:07,480 --> 00:01:10,680
+chemistry to study biological processes at the 
+
+16
+00:01:10,680 --> 00:01:15,160
+cellular and molecular level هي تطبيق كيميائي 
+
+17
+00:01:15,160 --> 00:01:21,440
+لدراسة العمليات الحيوية في داخل الخلايا تطبيق 
+
+18
+00:01:21,440 --> 00:01:27,060
+كيميائي لدراسة العمليات الحيوية في داخل الخلايا 
+
+19
+00:01:28,100 --> 00:01:31,500
+بحكي لي كمان إن الـ biochemistry is also concerned
+
+20
+00:01:31,500 --> 00:01:34,800
+with the study of structure and function of 
+
+21
+00:01:34,800 --> 00:01:39,100
+cellular components إن الكيميا الحيوية بتركز على 
+
+22
+00:01:39,100 --> 00:01:47,580
+دراسة التركيب والوظيفة هي تطبيق يركز على دراسة 
+
+23
+00:01:47,580 --> 00:01:50,700
+التركيب والوظيفة Structural and Function of 
+
+24
+00:01:50,700 --> 00:01:54,360
+Cellular Components اللي هي المكونات الخلوية تركز
+
+25
+00:01:54,360 --> 00:01:58,960
+على دراسة التركيب ووظيفة المكونات الخلوية زي إيش 
+
+26
+00:01:58,960 --> 00:02:02,600
+المكونات الخلوية؟ زي الـ protein، carbohydrates
+
+27
+00:02:02,600 --> 00:02:08,570
+واللبيد اللي هنتعرف عليهم لقدام الـ biochemistry
+
+28
+00:02:08,570 --> 00:02:12,890
+مستنتج من هذه التعريف إنها عبارة عن both life 
+
+29
+00:02:12,890 --> 00:02:17,800
+science and chemical science إنها علم كيميائي وعلم 
+
+30
+00:02:17,800 --> 00:02:22,700
+حيوي هي علم كيميائي وعلم حيوي في داخل أجسام 
+
+31
+00:02:22,700 --> 00:02:26,160
+الكائنات الحية عبارة عن دراسة التفاعلات الكيميائية 
+
+32
+00:02:26,160 --> 00:02:30,860
+هي علم كيميائي وعلم حيوي life science and chemical 
+
+33
+00:02:30,860 --> 00:02:35,000
+science it has provided explanation for the causes
+
+34
+00:02:35,000 --> 00:02:39,640
+of many diseases in humans بتخلينا نتعرف على 
+
+35
+00:02:39,640 --> 00:02:44,200
+مسببات الأمراض من خلال الكيميا الحيوية بقدر أعرف 
+
+36
+00:02:44,200 --> 00:02:49,240
+مسببات أمرض مثلا إن عندي خلل فيه البروتين لو ما 
+
+37
+00:02:49,240 --> 00:02:52,020
+فيش مستوى البروتين لاقيت فيه خلل فيه فبعرف إن
+
+38
+00:02:52,020 --> 00:02:57,820
+فيه منطقة ما بتتُصنع بشكل صحيح Biochemistry is the 
+
+39
+00:02:57,820 --> 00:03:00,420
+study of molecular structure and interaction
+
+40
+00:03:00,420 --> 00:03:03,770
+living organisms حكي هنا إنها بتدرس التركيب
+
+41
+00:03:03,770 --> 00:03:07,270
+والوظيفة الـ structure و الـ function للمكونات 
+
+42
+00:03:07,270 --> 00:03:11,350
+الخلوية Biochemists اللي هم الكيميائيين أخصائي 
+
+43
+00:03:11,350 --> 00:03:18,510
+الكيميا seek to understand يسعوا إلى فهم الأجسام من 
+
+44
+00:03:18,510 --> 00:03:23,550
+خلال التفاعلات الكيميائية biochemist seek to 
+
+45
+00:03:23,550 --> 00:03:26,730
+understand living organism in term of chemical 
+
+46
+00:03:26,730 --> 00:03:31,430
+reaction أخصائي الكيميا يسعى إلى فهم طبيعة الأجسام 
+
+47
+00:03:31,430 --> 00:03:37,270
+من خلال الـ chemical reaction نيجي نتعرف على أهداف 
+
+48
+00:03:37,270 --> 00:03:42,770
+دراسة المقرر ليش أنا بدرس مقرر الكيميا الحيوية؟
+
+49
+00:03:42,770 --> 00:03:46,510
+objective of this course أول شيء to learn 
+
+50
+00:03:46,510 --> 00:03:49,330
+fundamental technique used in biochemistry
+
+51
+00:03:49,330 --> 00:03:53,830
+لمعرفة التقنيات اللي بتفيد في الكيميا الحيوية
+
+52
+00:03:53,830 --> 00:03:59,410
+إيش أنا بستخدم تقنيات في الكيميا الحيوية؟ ثانيًا to 
+
+53
+00:03:59,410 --> 00:04:02,930
+learn some biochemical procedure and experiments
+
+54
+00:04:02,930 --> 00:04:06,970
+commonly used in biochemistry laboratory عشان أعرف 
+
+55
+00:04:06,970 --> 00:04:11,230
+الخطوات الـ procedure يعني الخطوات اللي بستخدمها 
+
+56
+00:04:11,230 --> 00:04:16,230
+لإجراء التجارب المختلفة أيش الخطوات المتبعة لإجراء 
+
+57
+00:04:16,230 --> 00:04:21,670
+التجارب المختلفة ثالثًا إشي to provide a basis for 
+
+58
+00:04:21,670 --> 00:04:25,090
+understanding how biochemical data obtained
+
+59
+00:04:25,090 --> 00:04:31,470
+analyzed and presented عشان أعرف القواعد لتحليل 
+
+60
+00:04:31,470 --> 00:04:37,310
+النتائج وأعرضها to provide a basis عشان تزودنا 
+
+61
+00:04:37,310 --> 00:04:43,770
+بالقواعد لفهم المعلومات وتحليل النتائج هذه الأهداف
+
+62
+00:04:43,770 --> 00:04:44,610
+المقرر
+
+63
+00:04:47,260 --> 00:04:51,880
+بالنسبة لقواعد السلامة والأمان داخل المعمل بيه الـ 
+
+64
+00:04:51,880 --> 00:04:56,400
+safety rules قواعد السلامة والأمان داخل المعمل
+
+65
+00:04:56,400 --> 00:05:03,060
+عشان أحمي نفسي فبدنا نتعرف على قواعد السلامة أول 
+
+66
+00:05:03,060 --> 00:05:07,040
+شيء لازم ألبس الـ coat الـ coat بدي يكون طويل طوله
+
+67
+00:05:07,040 --> 00:05:12,460
+مناسب الشالة بحطها في داخل الـ coat مخليهاش لبرا بدي 
+
+68
+00:05:12,460 --> 00:05:16,520
+ألبس gloves قبل لا أشتغل بعد هيك إيش بيحكيلي 
+
+69
+00:05:16,520 --> 00:05:19,780
+listen to or read instruction carefully before
+
+70
+00:05:19,780 --> 00:05:24,440
+attempting to do anything بدنا نستمع ونقرأ جيدا 
+
+71
+00:05:24,440 --> 00:05:28,940
+الخطوات أكثر من مرة وأشوف المواد اللي أنا بحتاجها
+
+72
+00:05:28,940 --> 00:05:34,000
+وأحضّر كل شيء clean up your lab area at the 
+
+73
+00:05:34,000 --> 00:05:39,050
+conclusion of the laboratory period بعد ما أخلص من
+
+74
+00:05:39,050 --> 00:05:43,050
+الشغل وكل شيء بدي أنظّف ورايا يعني الـ «بنش» بدي 
+
+75
+00:05:43,050 --> 00:05:48,410
+أمسحها إذا خلصت إذا فيها نبيب بدي أغسلها إذا 
+
+76
+00:05:48,410 --> 00:05:53,050
+الأدوات اللي أنا استخدمتها بدي أرجعها لمكانها بدي 
+
+77
+00:05:53,050 --> 00:05:56,990
+أرتّب كل شيء مكانه after handling chemical remove
+
+78
+00:05:56,990 --> 00:05:59,970
+gloves and always wash your hands with soap and
+
+79
+00:05:59,970 --> 00:06:04,210
+water بعد ما أخلص خلصت كل الشغل بدي أشيل الـ gloves
+
+80
+00:06:04,210 --> 00:06:07,130
+وبدي أغسل إيدي بالماء والصابون 
+
+81
+00:06:09,630 --> 00:06:13,610
+هنا هنا قاعدة بتحكي لي never trust any chemical you 
+
+82
+00:06:13,610 --> 00:06:16,930
+should never trust anything in the lab ممنوع نلمس
+
+83
+00:06:16,930 --> 00:06:22,050
+أي مادة كيميائية أو أي مادة موجودة داخل المعمل لأي 
+
+84
+00:06:22,050 --> 00:06:27,390
+سبب إن كان ممنوع نلمس أي مادة كيميائية كمان ممنوع 
+
+85
+00:06:27,390 --> 00:06:32,810
+الأكل والشرب في داخل المعمل ممنوع منعا باتًا للأكل 
+
+86
+00:06:32,810 --> 00:06:39,620
+والشرب داخل المعمل فيه هنا هنا حاجة بنسميها sign
+
+87
+00:06:39,620 --> 00:06:44,180
+for caution اللي هي إشارات الخطر إشارات الخطر هذه 
+
+88
+00:06:44,180 --> 00:06:49,520
+عبارة عن رموز استخدمت لتسهيل التعامل بين الشركات 
+
+89
+00:06:49,520 --> 00:06:55,060
+أو البلدان المختلفة عشان اللغة إشارات استعملة
+
+90
+00:06:55,060 --> 00:06:59,240
+لتسهيل التعامل تكون محطوطة على زجاجة المادة 
+
+91
+00:06:59,240 --> 00:07:03,240
+الكيميائية فهو الإشارة flammable معناها قابل
+
+92
+00:07:03,240 --> 00:07:07,480
+للاشتعال إن المادة الكيميائية هي قابلة للاشتعال
+
+93
+00:07:07,480 --> 00:07:11,920
+فلازم أبعدها عن أي مصدر لهب الإشارة الثانية بتدل
+
+94
+00:07:11,920 --> 00:07:15,400
+على إنها poison يعني إنها سامة إن هذه المادة سامة
+
+95
+00:07:15,400 --> 00:07:20,700
+الإشارة الثالثة biohazard إنها خطر على الحياة إن
+
+96
+00:07:20,700 --> 00:07:24,840
+هذه المادة خطر فيها infection فيها عدوى مثلا زي
+
+97
+00:07:24,840 --> 00:07:30,110
+بكتيريا أو فيروس أو فطريات الإشارة الرابعة معناها
+
+98
+00:07:30,110 --> 00:07:33,870
+corrosive corrosive معناها أنها مادة أكالة يعني
+
+99
+00:07:33,870 --> 00:07:38,370
+بتسبب تآكل الجلد لو أجي على الجلد بتسبب التآكل 
+
+100
+00:07:38,370 --> 00:07:43,390
+الإشارة الأخيرة معناها radioactive radioactive
+
+101
+00:07:43,390 --> 00:07:49,510
+يعني إشعاع يعني أنها مادة مشعة إن هذه المادة مشعة
+
+102
+00:07:51,050 --> 00:07:54,890
+بنحكي عن حاجة بنسميها chemical hazard chemical
+
+103
+00:07:54,890 --> 00:07:59,670
+hazard معناها مخاطر كيميائية ما أني بتعامل مع مواد 
+
+104
+00:07:59,670 --> 00:08:04,430
+كيميائية بوجه فيه مخاطر ممكن تصير معايا أول شيء 
+
+105
+00:08:04,430 --> 00:08:09,090
+بيحكي لي never mix chemical together يعني ممنوع
+
+106
+00:08:09,090 --> 00:08:13,810
+نخلط المواد الكيميائية مع بعض إلا إذا أنا حكيت لك
+
+107
+00:08:13,810 --> 00:08:18,610
+اخلطي المادة هذه على هذه ممنوع أنتي تخلطي مادة 
+
+108
+00:08:18,610 --> 00:08:23,430
+كيميائية على الثانية وإذا حكيت لك اخلطي مادة واحد
+
+109
+00:08:23,430 --> 00:08:27,470
+على مادة اثنين يعني تحطي واحد على اثنين مش هحط 
+
+110
+00:08:27,470 --> 00:08:31,130
+اثنين على واحد زي ما حكيت لك على الترتيب بتعملي
+
+111
+00:08:31,130 --> 00:08:36,850
+العكس ما ينفعش لإن هذه مواد كيميائية حاجة ثانية ما ينفع
+
+112
+00:08:36,850 --> 00:08:41,590
+pure water and concentrated acid ما ينفعش أضيف المي
+
+113
+00:08:41,590 --> 00:08:45,990
+على الحمض إذا بدي أحط مي على الحمض بحط الحمض على 
+
+114
+00:08:45,990 --> 00:08:53,730
+الماء مش الماء على الحمض الحمض يضاف على الماء وليس
+
+115
+00:08:53,730 --> 00:09:00,350
+الماء على الحمض ثالثًا شيء بيحكي لي check labels on
+
+116
+00:09:00,350 --> 00:09:05,010
+container twice to make sure you use the right
+
+117
+00:09:05,010 --> 00:09:10,380
+chemical لما بدي أستخدم مادة كيميائية بيحكي لي تأكدي 
+
+118
+00:09:10,380 --> 00:09:14,440
+واقرئي مرة واثنين الإشارة أو الورقة اللي بتكون
+
+119
+00:09:14,440 --> 00:09:17,480
+مكتوبة على الـ bottle عشان أتأكّد إن هذه المادة 
+
+120
+00:09:17,480 --> 00:09:21,860
+الصّح اللي أنا بدي إياها وبدي أتأكّد كمان من تاريخ 
+
+121
+00:09:21,860 --> 00:09:24,960
+الإنتاج وتاريخ الانتهاء وإذا في إشارات خطر
+
+122
+00:09:24,960 --> 00:09:28,950
+موجودة على الـ bottle إذا بما إني بتعامل مع مواد
+
+123
+00:09:28,950 --> 00:09:32,830
+كيميائية ممكن تواجهني مخاطر كيميائية فأنا بدي
+
+124
+00:09:32,830 --> 00:09:39,830
+أكون حريصة وألتزم بكل التعليمات طب إذا أنا عندي 
+
+125
+00:09:39,830 --> 00:09:44,010
+حادثة كيميائية وضروري أنا بدي أعرف شو هي ممنوع 
+
+126
+00:09:44,010 --> 00:09:45,370
+إحنا حكينا نتوقع
+
+127
+00:09:49,560 --> 00:09:53,460
+لو احتجنا if you need to smell the odor of a 
+
+128
+00:09:53,460 --> 00:09:58,540
+chemical كيف بتعملي برجع الزجاجة أو الـ bottle للخلف
+
+129
+00:10:04,380 --> 00:10:09,320
+بحط إيدي عند الفوهة كف الإيد وبعمل تقريب للأبخرة
+
+130
+00:10:09,320 --> 00:10:14,340
+تجيني أبخرة مخففة عن ناحية الأنف وبشمها زي
+
+131
+00:10:14,340 --> 00:10:18,120
+الصورة الموجودة عندنا كيف حط إيده حط إيده على
+
+132
+00:10:18,120 --> 00:10:21,660
+الفوهة بيعمل تقريب لأبخرة مخففة عن ناحية الأنف
+
+133
+00:10:21,660 --> 00:10:26,590
+عشان يشمّ الريحة طيب before leaving the lab قبل ما
+
+134
+00:10:26,590 --> 00:10:31,130
+أغادر من المعمل لازم أعمل workplace reagent and 
+
+135
+00:10:31,130 --> 00:10:34,310
+equipment should be put in order يعني ده رتّب
+
+136
+00:10:34,310 --> 00:10:37,550
+المعمل ورايا بدي أرجع المواد اللي استخدمتها و
+
+137
+00:10:37,550 --> 00:10:41,850
+الأدوات وأرتّبها زي ما كانت ومش ده لعبة glass بدي
+
+138
+00:10:41,850 --> 00:10:46,050
+أغسل الأدوات اللي استخدمتها وبدي أ .. إذا استخدمت 
+
+139
+00:10:46,050 --> 00:10:52,310
+الغاز بدي أسكر أمام الغاز وبدي أشتغل كفريق team 
+
+140
+00:10:52,310 --> 00:10:58,930
+work  بيحكي لي هنا الأدوات اللي ممكن أستخدمها أولًا شيء 
+
+141
+00:10:58,930 --> 00:11:02,290
+the only type of glassware that may safely be 
+
+142
+00:11:02,290 --> 00:11:06,710
+heated is either pyrex or kimax  إنه أنا هستخدم
+
+143
+00:11:06,710 --> 00:11:11,150
+زجاجيات هضطر لتسخين المواد اللي أنا بحتاجها فإذا
+
+144
+00:11:11,150 --> 00:11:15,730
+بدي أسخّنها فالأكثر أمان أن أستخدم مواد مصنوعة من 
+
+145
+00:11:15,730 --> 00:11:19,810
+مادة الـ pyrex أو الـ kimax هدول مواد مقاومة
+
+146
+00:11:19,810 --> 00:11:24,130
+للحرارة إذا عرضتها للهب بتكون مقاومة للحرارة طيب 
+
+147
+00:11:24,130 --> 00:11:27,330
+النقطتين اللي بعد هيك بتحكي لي إنه أنا لو بدي أسخّن 
+
+148
+00:11:27,330 --> 00:11:31,110
+المادة إيش بدي أعمل؟ كيف بدي أسخّنها؟ أجيت المادة
+
+149
+00:11:31,110 --> 00:11:35,190
+بحطها في الأنبوب المصنوعة من مواد مقاومة للحرارة
+
+150
+00:11:35,190 --> 00:11:39,030
+اللّهب باجي لمصدر اللّهب لازم يكون في وسط الـ bench
+
+151
+00:11:39,030 --> 00:11:44,530
+ما يكونش على الطرف فأشغل اللّهب وبعدين باجي للأنبوب
+
+152
+00:11:44,530 --> 00:11:48,970
+اللي أنا بدي أسخّنها فامسكها بملقط خشبي أو مصنوع من
+
+153
+00:11:48,970 --> 00:11:53,940
+الستانلس و بمسك القنبوبة في هذا الملقطة وبحطها على
+
+154
+00:11:53,940 --> 00:11:58,900
+اللّهب بشكل مائل وبضمن أحرك فيها بركزهاش على اللّهب
+
+155
+00:11:58,900 --> 00:12:04,860
+ولا بحطها بشكل عمودي وإنما بشكل مائل وبضمن أحرك 
+
+156
+00:12:04,860 --> 00:12:10,120
+فيها لحد ما تسخّن وبشيلها للوقت المحدد الفتحة تاعة 
+
+157
+00:12:10,120 --> 00:12:11,980
+الأنبوب بتكون على 
+
+158
+00:12:16,030 --> 00:12:20,630
+الجانب مش باتجاهي أو باتجاه زميلي لإن لو غلطت 
+
+159
+00:12:20,630 --> 00:12:24,470
+المادة الكيميائية وطلعت ما تطلعش في وجه أحد أو 
+
+160
+00:12:24,470 --> 00:12:30,120
+يتأذّى أحد ما هنحكي عن بعض الأدوات اللي هنستخدمها
+
+161
+00:12:30,120 --> 00:12:35,700
+أول أداة اسمها volumetric flask volumetric flask 
+
+162
+00:12:35,700 --> 00:12:40,040
+مبوق زجاجي بناخد فيه حجم محدد بتكون في عندها علامة 
+
+163
+00:12:40,040 --> 00:12:43,800
+فوق اللي هي الإشارة إذا عبّت لعندها معناها دي سعتها 
+
+164
+00:12:43,800 --> 00:12:47,600
+كل الـ volumetric flask كل مكتوب عليها سعتها 250، 
+
+165
+00:12:47,600 --> 00:12:51,580
+500، 150، اللي عند العلامة اللي عبيها يعني هذه 
+
+166
+00:12:51,580 --> 00:12:56,340
+السعة الليلة اسمها volumetric flask في عندنا 
+
+167
+00:12:56,340 --> 00:13:00,480
+dropper في عندنا كمان test tube لنحكي عن بعض 
+
+168
+00:13:00,480 --> 00:13:04,360
+الأدوات اللي هنستخدمها volumetric flask مبوق زجاجي
+
+169
+00:13:04,360 --> 00:13:08,200
+علامة فوق اللي هي الإشارة إذا عبّت لعندها معناها 
+
+170
+00:13:08,200 --> 00:13:11,260
+دي سعتها كل الـ volumetric flask كل مكتوب عليها 
+
+171
+00:13:11,260 --> 00:13:16,360
+سعتها 250، 500، 150، اللي عند العلامة معبيها يعني 
+
+172
+00:13:16,360 --> 00:13:21,350
+هذه السعة الليلة اسمها volumetric flask في عندنا
+
+173
+00:13:21,350 --> 00:13:26,170
+dropper  الـ dropper هذه باخد فيه حجوم عشوائية في عندنا 
+
+174
+00:13:26,170 --> 00:13:31,110
+balance ميزان أكيد بوزن فيه في عندنا سيلندر هذا
+
+175
+00:13:31,110 --> 00:13:36,820
+عبارة عن أنبوب أسطواني مدرج باخد فيه حجم في عندنا 
+
+176
+0
\ No newline at end of file
diff --git a/PL9fwy3NUQKwZ1Q7zOg1ObgufPMjRI3WY1/vUaw7lH8dOs_raw.json b/PL9fwy3NUQKwZ1Q7zOg1ObgufPMjRI3WY1/vUaw7lH8dOs_raw.json
new file mode 100644
index 0000000000000000000000000000000000000000..74916488bf7381750bc0592eac777dd38aba2d8a
--- /dev/null
+++ b/PL9fwy3NUQKwZ1Q7zOg1ObgufPMjRI3WY1/vUaw7lH8dOs_raw.json
@@ -0,0 +1 @@
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1.0}, {"id": 9, "seek": 18272, "start": 162.22, "end": 182.72, "text": "من خلال الكيميا الحيوية بقدر أعرف مسببات أمرها مثلا إن عندي خلال فيه البروتين لو ما جيست مستوى البروتين لاجيت فيه خلال فيه فبعرف إن فيه منطقة لاتصنع بشكل صحيح Biochemistry is the study of molecular structure and interaction living organisms حكي هنا إنها بتدرس", "tokens": [27842, 16490, 1211, 6027, 33251, 32640, 25528, 21542, 1829, 2407, 10632, 4724, 28543, 2288, 5551, 3615, 28480, 3714, 35457, 3555, 9307, 5551, 29973, 11296, 50113, 15040, 36145, 18871, 16254, 16490, 1211, 6027, 8978, 3224, 2423, 26890, 35473, 9957, 45164, 19446, 10874, 1829, 14851, 3714, 14851, 2407, 7578, 2423, 26890, 35473, 9957, 5296, 26108, 36081, 8978, 3224, 16490, 1211, 6027, 8978, 3224, 6156, 3555, 3615, 28480, 36145, 8978, 3224, 9154, 9566, 28671, 5296, 9307, 9381, 1863, 3615, 4724, 8592, 28820, 20328, 5016, 1829, 5016, 26840, 48353, 307, 264, 2979, 295, 19046, 3877, 293, 9285, 2647, 22110, 11331, 4117, 1829, 34105, 36145, 11296, 39894, 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بين الشركات أو البلدان المختلفة عشان", "tokens": [41185, 3224, 34105, 34105, 11331, 26108, 3660, 44945, 38251, 1829, 11296, 1465, 337, 23585, 13672, 1829, 39896, 11933, 8592, 9640, 9307, 33962, 9566, 2288, 11933, 8592, 9640, 9307, 33962, 9566, 2288, 29538, 6225, 3555, 9640, 3660, 18871, 12602, 2304, 2407, 11622, 44713, 9778, 40448, 2655, 5296, 2655, 3794, 3224, 26895, 16712, 3615, 10943, 1211, 49374, 25124, 31747, 9307, 34051, 29739, 1211, 3215, 7649, 9673, 46456, 46538, 3660, 6225, 8592, 7649], "avg_logprob": -0.12389964830707496, "compression_ratio": 1.592814371257485, "no_speech_prob": 3.8743019104003906e-05, "words": [{"start": 397.0, "end": 397.42, "word": "فيه", "probability": 0.544921875}, {"start": 397.42, "end": 397.58, "word": " هنا", "probability": 0.7548828125}, {"start": 397.58, "end": 397.82, "word": " هنا", "probability": 0.6728515625}, {"start": 397.82, "end": 398.26, "word": " حاجة", "probability": 0.990234375}, {"start": 398.26, "end": 399.04, "word": " بنسميها", 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"temperature": 1.0}, {"id": 39, "seek": 86134, "start": 837.3, "end": 861.34, "text": "هنا كمان test tube brush اللى هى الفرشاية اللى بنظف فيها القنابيب بعد ما اخلص من الشغل بتهى نظف القنبوب بداخل الفرشاية بنظفها فهى ان حاجة بنسميها clamps اللى هو الملقط اللى بمسك فيه القنابيب لما نسخنها فهى ان حاجة بنسميها rack تحتوي على فتحات مناسبة للقنابيب بقعد فيه القنابيب بحط القنابيب فيه بوقفها", "tokens": [3224, 8315, 9122, 2304, 7649, 1500, 9917, 5287, 13672, 7578, 8032, 7578, 27188, 2288, 8592, 995, 10632, 13672, 7578, 44945, 19913, 5172, 8978, 11296, 25062, 8315, 21292, 3555, 39182, 19446, 1975, 9778, 1211, 9381, 9154, 25124, 17082, 1211, 39894, 3224, 7578, 8717, 19913, 5172, 25062, 1863, 3555, 37746, 47525, 47283, 1211, 27188, 2288, 8592, 995, 10632, 44945, 19913, 5172, 11296, 6156, 3224, 7578, 16472, 11331, 26108, 3660, 44945, 38251, 1829, 11296, 44423, 13672, 7578, 31439, 9673, 1211, 47432, 13672, 7578, 4724, 2304, 3794, 4117, 8978, 3224, 25062, 8315, 21292, 3555, 5296, 15042, 8717, 3794, 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0.9718424479166666}, {"start": 844.5, "end": 845.06, "word": " القنبوب", "probability": 0.75433349609375}, {"start": 845.06, "end": 845.78, "word": " بداخل", "probability": 0.8492838541666666}, {"start": 845.78, "end": 846.32, "word": " الفرشاية", "probability": 0.988671875}, {"start": 846.32, "end": 847.06, "word": " بنظفها", "probability": 0.9246826171875}, {"start": 847.06, "end": 847.68, "word": " فهى", "probability": 0.706298828125}, {"start": 847.68, "end": 847.78, "word": " ان", "probability": 0.24365234375}, {"start": 847.78, "end": 848.04, "word": " حاجة", "probability": 0.9187825520833334}, {"start": 848.04, "end": 848.5, "word": " بنسميها", "probability": 0.873291015625}, {"start": 848.5, "end": 849.12, "word": " clamps", "probability": 0.7265625}, {"start": 849.12, "end": 849.52, "word": " اللى", "probability": 0.943603515625}, {"start": 849.52, "end": 849.64, "word": " هو", "probability": 0.75}, {"start": 849.64, "end": 850.3, "word": " الملقط", "probability": 0.8357747395833334}, {"start": 850.3, "end": 850.46, "word": " اللى", "probability": 0.959716796875}, {"start": 850.46, "end": 850.84, "word": " بمسك", "probability": 0.9381103515625}, {"start": 850.84, "end": 851.02, "word": " فيه", "probability": 0.97216796875}, {"start": 851.02, "end": 851.46, "word": " القنابيب", "probability": 0.952392578125}, {"start": 851.46, "end": 851.7, "word": " لما", "probability": 0.970703125}, {"start": 851.7, "end": 852.74, "word": " نسخنها", "probability": 0.839892578125}, {"start": 852.74, "end": 853.44, "word": " فهى", "probability": 0.75830078125}, {"start": 853.44, "end": 853.54, "word": " ان", "probability": 0.265625}, {"start": 853.54, "end": 853.78, "word": " حاجة", "probability": 0.9930013020833334}, {"start": 853.78, "end": 854.42, "word": " بنسميها", "probability": 0.9664306640625}, {"start": 854.42, "end": 854.88, "word": " rack", "probability": 0.487060546875}, {"start": 854.88, "end": 855.72, "word": " تحتوي", "probability": 0.83984375}, {"start": 855.72, "end": 855.98, "word": " على", "probability": 0.89599609375}, {"start": 855.98, "end": 856.62, "word": " فتحات", "probability": 0.9683837890625}, {"start": 856.62, "end": 857.48, "word": " مناسبة", "probability": 0.949951171875}, {"start": 857.48, "end": 858.4, "word": " للقنابيب", "probability": 0.941796875}, {"start": 858.4, "end": 858.98, "word": " بقعد", "probability": 0.6864420572916666}, {"start": 858.98, "end": 859.22, "word": " فيه", "probability": 0.828125}, {"start": 859.22, "end": 859.56, "word": " القنابيب", "probability": 0.96875}, {"start": 859.56, "end": 859.92, "word": " بحط", "probability": 0.9488932291666666}, {"start": 859.92, "end": 860.42, "word": " القنابيب", "probability": 0.9669189453125}, {"start": 860.42, "end": 860.64, "word": " فيه", "probability": 0.959228515625}, {"start": 860.64, "end": 861.34, "word": " بوقفها", "probability": 0.782958984375}], "temperature": 1.0}, {"id": 40, "seek": 86783, "start": 863.49, "end": 867.83, "text": "اجهزة 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\ No newline at end of file
diff --git a/PL9fwy3NUQKwZ1Q7zOg1ObgufPMjRI3WY1/vUaw7lH8dOs_raw.srt b/PL9fwy3NUQKwZ1Q7zOg1ObgufPMjRI3WY1/vUaw7lH8dOs_raw.srt
new file mode 100644
index 0000000000000000000000000000000000000000..4d754bdb8f22e2ed6aa5dd7e673ad9cce37977b5
--- /dev/null
+++ b/PL9fwy3NUQKwZ1Q7zOg1ObgufPMjRI3WY1/vUaw7lH8dOs_raw.srt
@@ -0,0 +1,748 @@
+1
+00:00:01,150 --> 00:00:05,090
+اليوم إن شاء الله راح نحكي في أول معمل من معامل
+
+2
+00:00:05,090 --> 00:00:09,690
+الكيميا الحيوية هيكون عبارة عن مقدمة عامة عن
+
+3
+00:00:09,690 --> 00:00:14,390
+المادة تعريف الكيميا الحيوية الأهداف العامة من
+
+4
+00:00:14,390 --> 00:00:20,150
+دراسة المصاق وليش احنا بندرسه هنتعرف فيه على ال
+
+5
+00:00:20,150 --> 00:00:23,910
+safe rule داخل المعمل قواعد السلامة والأمان داخل
+
+6
+00:00:23,910 --> 00:00:28,220
+المعملهنتعرف فيه على بعض الأدوات اللي هنستخدمها في
+
+7
+00:00:28,220 --> 00:00:34,100
+المعمل و هندخل في مقدمة بسيطة عن الأحماض الأمينية
+
+8
+00:00:34,100 --> 00:00:40,520
+في البداية خلينا نتعرف على تعريف ال biochemistry
+
+9
+00:00:40,520 --> 00:00:44,930
+اللي هي الكيميا الحيويةلما نحكي كيميا حيوية، إيش
+
+10
+00:00:44,930 --> 00:00:49,450
+بنستنتج منها؟ كيميا، كلمة كيميا معناها تفاعلات
+
+11
+00:00:49,450 --> 00:00:54,270
+كيميائية بدها تكون حيوية يعني تحدث في داخل جسم
+
+12
+00:00:54,270 --> 00:00:58,950
+الإنسان أو جسم الكائن الحي فهي تفاعلات كيميائية
+
+13
+00:00:58,950 --> 00:01:03,820
+تحدث في داخل أجسام الكائنات الحيةمش مكتوب هنا
+
+14
+00:01:03,820 --> 00:01:07,480
+مكتوب ان ال biochemistry is the application of
+
+15
+00:01:07,480 --> 00:01:10,680
+chemistry to study biological processes at the
+
+16
+00:01:10,680 --> 00:01:15,160
+cellular and molecular level هي تطبيق كيميائي
+
+17
+00:01:15,160 --> 00:01:21,440
+لدراسة العمليات الحيوية في داخل الخلايا تطبيق
+
+18
+00:01:21,440 --> 00:01:27,060
+كيميائي لدراسة العمليات الحيوية في داخل الخلايا
+
+19
+00:01:28,100 --> 00:01:31,500
+بحكي لي كمان إن ال biochemistry is also concerned
+
+20
+00:01:31,500 --> 00:01:34,800
+with the study of structure and function of
+
+21
+00:01:34,800 --> 00:01:39,100
+cellular components إن الكيميا الحيوية بتركز على
+
+22
+00:01:39,100 --> 00:01:47,580
+دراسة التركيب والوظيفةهي تطبيق يركز على دراسة
+
+23
+00:01:47,580 --> 00:01:50,700
+التركيب والوظيفة Structural and Function of
+
+24
+00:01:50,700 --> 00:01:54,360
+Cellular Components اللي هي المكونات الخلوية تركز
+
+25
+00:01:54,360 --> 00:01:58,960
+على دراسة التركيب ووظيفة المكونات الخلوية زي إيش
+
+26
+00:01:58,960 --> 00:02:02,600
+المكونات الخلوية؟ زي الـ protein، carbohydrates
+
+27
+00:02:02,600 --> 00:02:08,570
+واللبن اللي هنتعرف عليهم لقدامال biochemistry
+
+28
+00:02:08,570 --> 00:02:12,890
+مستنتج من هذه التعريف إنها عبارة عن both life
+
+29
+00:02:12,890 --> 00:02:17,800
+science and chemical scienceإنها علم كيميائي وعلم
+
+30
+00:02:17,800 --> 00:02:22,700
+حيوي هي علم كيميائي وعلم حيوي في داخل أجسام
+
+31
+00:02:22,700 --> 00:02:26,160
+الكائنات الحية عبارة عن دراسة التفاعلات الكيميائية
+
+32
+00:02:26,160 --> 00:02:30,860
+هي علم كيميائي وعلم حيوي life science and chemical
+
+33
+00:02:30,860 --> 00:02:35,000
+science it has provided explanation for the causes
+
+34
+00:02:35,000 --> 00:02:39,640
+of many diseases in humans بتخلينا نتعرف على
+
+35
+00:02:39,640 --> 00:02:44,200
+مسببات الأمراضمن خلال الكيميا الحيوية بقدر أعرف
+
+36
+00:02:44,200 --> 00:02:49,240
+مسببات أمرها مثلا إن عندي خلال فيه البروتين لو ما
+
+37
+00:02:49,240 --> 00:02:52,020
+جيست مستوى البروتين لاجيت فيه خلال فيه فبعرف إن
+
+38
+00:02:52,020 --> 00:02:57,820
+فيه منطقة لاتصنع بشكل صحيح Biochemistry is the
+
+39
+00:02:57,820 --> 00:03:00,420
+study of molecular structure and interaction
+
+40
+00:03:00,420 --> 00:03:03,770
+living organisms حكي هنا إنها بتدرسالتركيب
+
+41
+00:03:03,770 --> 00:03:07,270
+والوظيفة الـ structure و الـ function للمكونات
+
+42
+00:03:07,270 --> 00:03:11,350
+الخلوية Biochemists اللي هم الكيميائيين أخصائي
+
+43
+00:03:11,350 --> 00:03:18,510
+الكيميا seek to understand يسعوا إلى فهمالأجسام من
+
+44
+00:03:18,510 --> 00:03:23,550
+خلال التفاعلات الكيمية biochemist seek to
+
+45
+00:03:23,550 --> 00:03:26,730
+understand living organism in term of chemical
+
+46
+00:03:26,730 --> 00:03:31,430
+reaction أخصائي الكيميا يسعى إلى فهم طبيعة الأجسام
+
+47
+00:03:31,430 --> 00:03:37,270
+من خلال ال chemical reactionنيجي نتعرف على أهداف
+
+48
+00:03:37,270 --> 00:03:42,770
+دراسة المصاق ليش أنا بدرس مصاق الكيميا الحيوية؟
+
+49
+00:03:42,770 --> 00:03:46,510
+objective of this course أول شيء to learn
+
+50
+00:03:46,510 --> 00:03:49,330
+fundamental technique used in biochemistry
+
+51
+00:03:49,330 --> 00:03:53,830
+لمعارفات التقنيات اللي بتفيد في الكيميا الحيوية
+
+52
+00:03:53,830 --> 00:03:59,410
+إيش أنا بستخدم تقنيات في الكيميا الحيوية؟تنين to
+
+53
+00:03:59,410 --> 00:04:02,930
+learn some biochemical procedure and experiments
+
+54
+00:04:02,930 --> 00:04:06,970
+commonly used in biochemistry laboratory عشان أعرف
+
+55
+00:04:06,970 --> 00:04:11,230
+الخطوات ال procedure يعني الخطوات اللي بستخدمها
+
+56
+00:04:11,230 --> 00:04:16,230
+لإجراء التجارب المختلفة أيش الخطوات المتبعة لإجراء
+
+57
+00:04:16,230 --> 00:04:21,670
+التجارب المختلفةتالت إشي to provide a basis for
+
+58
+00:04:21,670 --> 00:04:25,090
+understanding how biochemical data obtained
+
+59
+00:04:25,090 --> 00:04:31,470
+analyzed and presented عشان أعرف القواعد لتحليل
+
+60
+00:04:31,470 --> 00:04:37,310
+النتائج وأوضيها to provide a basis عشان تزودنا
+
+61
+00:04:37,310 --> 00:04:43,770
+بالقواعد لفهم المعلومات وتحليل النتائج هاي الأهداف
+
+62
+00:04:43,770 --> 00:04:44,610
+المصاق
+
+63
+00:04:47,260 --> 00:04:51,880
+بالنسبة لقواعد السلامة والأمان داخل المجتمع بيه ال
+
+64
+00:04:51,880 --> 00:04:56,400
+safety rules قواعد السلامة والأمان داخل المجتمع
+
+65
+00:04:56,400 --> 00:05:03,060
+عشان أحمي نفسي فبدنا نتعرف على قواعد السلامة أول
+
+66
+00:05:03,060 --> 00:05:07,040
+شي لازم ألبس ال coat ال coat بدي يكون طويل طوله
+
+67
+00:05:07,040 --> 00:05:12,460
+مناسبالشالة بحطها في داخل ال coat مخليهاش لبرا بدي
+
+68
+00:05:12,460 --> 00:05:16,520
+ألبس gloves قبل لا أشتغل بعد هيك إيش بيحكيلي
+
+69
+00:05:16,520 --> 00:05:19,780
+listen to or read instruction carefully before
+
+70
+00:05:19,780 --> 00:05:24,440
+attempting to do anything بدنا نستمع و نقرأ جيدا
+
+71
+00:05:24,440 --> 00:05:28,940
+الخطوات أكتر من مرة و أشوف المواد اللي أنا بحتاجها
+
+72
+00:05:28,940 --> 00:05:34,000
+و أحضر كل إشي clean up your lab area at the
+
+73
+00:05:34,000 --> 00:05:39,050
+conclusion of the laboratory periodبعد ما أخلص من
+
+74
+00:05:39,050 --> 00:05:43,050
+الشغل و كل شيء بدي أنظف ورايا يعني ال «بنش» بدي
+
+75
+00:05:43,050 --> 00:05:48,410
+أمسحها إذا خلصت إذا فيها نبيب بدي أغسلها إذا
+
+76
+00:05:48,410 --> 00:05:53,050
+الأدوات اللي أنا استخدمتها بدي أرجحها مكانها بدي
+
+77
+00:05:53,050 --> 00:05:56,990
+أرتب كل إشي مكاني after handling chemical remove
+
+78
+00:05:56,990 --> 00:05:59,970
+gloves and always wash your hands with soap and
+
+79
+00:05:59,970 --> 00:06:04,210
+water بعد ما أخلص خلصت كل الشغل بدي أشلح ال gloves
+
+80
+00:06:04,210 --> 00:06:07,130
+و بدي أغسل إيدي بالماء والصبون
+
+81
+00:06:09,630 --> 00:06:13,610
+هنا هنا قاعدة تحكي لي never trust any chemical you
+
+82
+00:06:13,610 --> 00:06:16,930
+should never trust anything in the lab ممنوع نطوق
+
+83
+00:06:16,930 --> 00:06:22,050
+أي مادة كيميائية أو أي مادة موجودة داخل المعمل لأي
+
+84
+00:06:22,050 --> 00:06:27,390
+سبب إن كان ممنوع نطوق أي مادة كيميائية كمان ممنوع
+
+85
+00:06:27,390 --> 00:06:32,810
+الأكل والشرب في داخل المعمل ممنوع منعم بادة للأكل
+
+86
+00:06:32,810 --> 00:06:39,620
+والشرب داخل المعملفيه هنا هنا حاجة بنسميها sign
+
+87
+00:06:39,620 --> 00:06:44,180
+for caution اللي هي إشارات الخطر إشارات الخطر هذه
+
+88
+00:06:44,180 --> 00:06:49,520
+عبارة عن رموز استخدمت لتسهيل التعامل بين الشركات
+
+89
+00:06:49,520 --> 00:06:55,060
+أو البلدان المختلفة عشاناللغة إشارات استعملة
+
+90
+00:06:55,060 --> 00:06:59,240
+لتسهيل التعامل تكون محطوطة على ججاجة المادة
+
+91
+00:06:59,240 --> 00:07:03,240
+الكيميائية فهو الإشارة flameable معناها قابل
+
+92
+00:07:03,240 --> 00:07:07,480
+للاشتعال إن المادة الكيميائية هي قابلة للاشتعال
+
+93
+00:07:07,480 --> 00:07:11,920
+فلازم أبعدها عن أي مصدر لهاالإشارة التانية بتدل
+
+94
+00:07:11,920 --> 00:07:15,400
+على إنها poison، يعني إنها سام، إن هذه المادة سام
+
+95
+00:07:15,400 --> 00:07:20,700
+الإشارة التالتة، biohazard، إنها خطر على الحياة إن
+
+96
+00:07:20,700 --> 00:07:24,840
+هذه المادة خطر، فيها infection، فيها عدوى مثلا زي
+
+97
+00:07:24,840 --> 00:07:30,110
+بكتيريا أو فيروس أو برزانالإشارة الرابعة معناها
+
+98
+00:07:30,110 --> 00:07:33,870
+corrosive corrosive معناها أنها مادة آكلة يعني
+
+99
+00:07:33,870 --> 00:07:38,370
+بتسبب تآكل الجلد لو أجي على الجلد بتسبب التآكل
+
+100
+00:07:38,370 --> 00:07:43,390
+الإشارة الأخيرة معناها radioactive radioactive
+
+101
+00:07:43,390 --> 00:07:49,510
+يعني إشعاع يعني أنها مادة مشعة أن هذه المادة مشعة
+
+102
+00:07:51,050 --> 00:07:54,890
+بنحكي عن حاجة بنسميها chemical hazard chemical
+
+103
+00:07:54,890 --> 00:07:59,670
+hazard معناها مخاطر كيميائية ما أني بتعامل مع مواد
+
+104
+00:07:59,670 --> 00:08:04,430
+كيميائية بجه فيه مخاطر ممكن تصير معايا أول إشي
+
+105
+00:08:04,430 --> 00:08:09,090
+بيحكيني never mix chemical together يعني ممنوع
+
+106
+00:08:09,090 --> 00:08:13,810
+نخلط المواد الكيميائية مع بعض إلا إذا أنا حكيتلك
+
+107
+00:08:13,810 --> 00:08:18,610
+اخلطي المادة هذه على هذه ممنوع أنتي تخلطي مادة
+
+108
+00:08:18,610 --> 00:08:23,430
+كيميائية على التانيةوإذا حكيتلك اخلطي مادة واحد
+
+109
+00:08:23,430 --> 00:08:27,470
+على مادة اتنين يعني تحطي واحد على اتنين مش هحط
+
+110
+00:08:27,470 --> 00:08:31,130
+اتنين على واحد زي ما حكيتلك على الترتيب بتعمل
+
+111
+00:08:31,130 --> 00:08:36,850
+العكس مينفعش لإن هاي مواد كيميائية حاجة تانية نفع
+
+112
+00:08:36,850 --> 00:08:41,590
+pure water and concentrated acid مينفعش أضيف المي
+
+113
+00:08:41,590 --> 00:08:45,990
+على الحمض إذا بدي أحط مي على الحمض بحط الحمض على
+
+114
+00:08:45,990 --> 00:08:53,730
+الماء مش الماء على الحمضالحمد يضاف على الماء وليس
+
+115
+00:08:53,730 --> 00:09:00,350
+الماء على الحمد تالت اشي بيحكيلي check labels on
+
+116
+00:09:00,350 --> 00:09:05,010
+container twice to make sure you use the right
+
+117
+00:09:05,010 --> 00:09:10,380
+chemicalلمّا بدي أستخدم مادة كيميائية بحكيلي تأكدي
+
+118
+00:09:10,380 --> 00:09:14,440
+و اقرأ مرة و اتنين الإشارة أو الورقة اللي بتكون
+
+119
+00:09:14,440 --> 00:09:17,480
+مكتوبة على الـ bottle عشان أتأكد أن هذه المادة
+
+120
+00:09:17,480 --> 00:09:21,860
+الصح اللي أنا بدي إياها و بدي أتأكد كمان من تاريخ
+
+121
+00:09:21,860 --> 00:09:24,960
+الإنتاج و تاريخ الانتهاء و إذا في إشارات خطر
+
+122
+00:09:24,960 --> 00:09:28,950
+موجودة على الـ bottleإذا بما إني بتعامل مع مواد
+
+123
+00:09:28,950 --> 00:09:32,830
+كيميائية، ممكن تواجهني مخاطر كيميائية، فأنا بدي
+
+124
+00:09:32,830 --> 00:09:39,830
+أكون حريصة و ألتزم بكل التعليمات طب إذا أنا عندي
+
+125
+00:09:39,830 --> 00:09:44,010
+مهنتة كيميائية و ضرورة أنا بدي أعرف شو هي، ممنوع
+
+126
+00:09:44,010 --> 00:09:45,370
+إحنا حاكينا نتوقع
+
+127
+00:09:49,560 --> 00:09:53,460
+لو احتجنا if you need to smell the odor of a
+
+128
+00:09:53,460 --> 00:09:58,540
+chemical كيف بتعمل برجع الججاجة أو البوتل للخلف
+
+129
+00:10:04,380 --> 00:10:09,320
+بحط إيدي عند الفوهة، كف الإيد و بعمل تقريب للأبخرة
+
+130
+00:10:09,320 --> 00:10:14,340
+تجيني أبخرة مخففة عن ناحية الفأنف و بشمها زي
+
+131
+00:10:14,340 --> 00:10:18,120
+الصورة الموجودة عندنا كيف حط إيده حط إيده على
+
+132
+00:10:18,120 --> 00:10:21,660
+الفوهة بيعمل تقريب لأبخرة مخففة عن ناحية الأنف
+
+133
+00:10:21,660 --> 00:10:26,590
+عشان يشم الريحةطيب before leaving the lab قبل ما
+
+134
+00:10:26,590 --> 00:10:31,130
+أغادر من المعمل لازم أعمل workplace reagent and
+
+135
+00:10:31,130 --> 00:10:34,310
+equipment should be put in order يعني ده رتب
+
+136
+00:10:34,310 --> 00:10:37,550
+المعمل وراي بدي أرجع المواد اللي استخدمتها و
+
+137
+00:10:37,550 --> 00:10:41,850
+الأدوات و أرتبها زي ما كانت و مش ده لعبة glass بدي
+
+138
+00:10:41,850 --> 00:10:46,050
+أخسم الأدوات اللي استخدمتها و بدي أ .. إذا استخدمت
+
+139
+00:10:46,050 --> 00:10:52,310
+الغاز بدي أسكس أمام الغاز و بدي أشتغل كفريق team
+
+140
+00:10:52,310 --> 00:10:58,930
+warبحكيلي هنا الأدوات اللي ممكن أستخدمها أولاشي
+
+141
+00:10:58,930 --> 00:11:02,290
+the only type of glassware that may safely be
+
+142
+00:11:02,290 --> 00:11:06,710
+heated is either chymex or pyrex أنه أنا هستخدم
+
+143
+00:11:06,710 --> 00:11:11,150
+زجاجيات هضطر لتسخين المواد اللي أنا بحتاجها فإذا
+
+144
+00:11:11,150 --> 00:11:15,730
+بدي أسخنها فالأكتر أمان أن أستخدم مواد مصنوعة من
+
+145
+00:11:15,730 --> 00:11:19,810
+مادة ال chymex أو ال pyrex هدول مواد مقاومة
+
+146
+00:11:19,810 --> 00:11:24,130
+للحرارة إذا عرضتها للهب بتكون مقاومة للحرارةطيب
+
+147
+00:11:24,130 --> 00:11:27,330
+النقطتين اللي بعد هيك بتحكوني أنه أنا لو بدي أسخن
+
+148
+00:11:27,330 --> 00:11:31,110
+المادة إيش بدي أعمل؟ كيف بدي أسخنها؟ أجيت المادة
+
+149
+00:11:31,110 --> 00:11:35,190
+بحطها في التيوب المصنعة من مواد مقاومة للحرارة
+
+150
+00:11:35,190 --> 00:11:39,030
+اللهب باجي لمصدر اللهب لازم يكون في وسط الbench
+
+151
+00:11:39,030 --> 00:11:44,530
+مايكونش على الطرف فأشغل اللهب و بعدين باجي للتيوب
+
+152
+00:11:44,530 --> 00:11:48,970
+اللي أنا بدي أسخنها فامسكها بملقط خشبي أو مصنوع من
+
+153
+00:11:48,970 --> 00:11:53,940
+الاستهلزو بمسك القنبوبة في هذا الملقطة و بحطها على
+
+154
+00:11:53,940 --> 00:11:58,900
+اللهب بشكل مائل و بضن أحرك فيها بركزهاش على اللهب
+
+155
+00:11:58,900 --> 00:12:04,860
+ولا بحطها بشكل عمودي و انما بشكل مائل و بضن أحرك
+
+156
+00:12:04,860 --> 00:12:10,120
+فيها لحد ما تسخن و بشيلها للوقت المحدد الفتحة تاعة
+
+157
+00:12:10,120 --> 00:12:11,980
+القنبوب بتكون على
+
+158
+00:12:16,030 --> 00:12:20,630
+المهم مش باتجاهي أو باتجاه زميلي لإن لو غلطت
+
+159
+00:12:20,630 --> 00:12:24,470
+المادة الكيميائية وطلعت ماتطلعش في وجه حدا أو
+
+160
+00:12:24,470 --> 00:12:30,120
+يتأذى حدامش هنحكي عن بعض الأدوات اللي هنستخدمها
+
+161
+00:12:30,120 --> 00:12:35,700
+أول أداة اسمها volumetric flask volumetric flask
+
+162
+00:12:35,700 --> 00:12:40,040
+مبوب زجاجي بناخد فيه حجم محدد بتكون في عنده علامة
+
+163
+00:12:40,040 --> 00:12:43,800
+فوق اللي هي الإشارة إذا عبّت لعندها معناها دي ساعة
+
+164
+00:12:43,800 --> 00:12:47,600
+كل ال volumetric flask كل مكتوب عليها ساعتها 250،
+
+165
+00:12:47,600 --> 00:12:51,580
+500، 150، اللي عند العلامة اللي عبيها يعني هذه
+
+166
+00:12:51,580 --> 00:12:56,340
+الساعة الليلةاسمها volumetric flask في عندنا
+
+167
+00:12:56,340 --> 00:13:00,480
+dropper، في عندنا كمان test tube لنحكي عن بعض
+
+168
+00:13:00,480 --> 00:13:04,360
+الأدوات اللي هنستخدمها volumetric flask مبوق زجاجي
+
+169
+00:13:04,360 --> 00:13:08,200
+علامة فوق اللي هي الإشارة إذا عبّت لعندها معناها
+
+170
+00:13:08,200 --> 00:13:11,260
+دي ساعة كل ال volumetric flask كل مكتوب عليها
+
+171
+00:13:11,260 --> 00:13:16,360
+ساعتها 250، 500، 150، اللي عند العلمة معبيها يعني
+
+172
+00:13:16,360 --> 00:13:21,350
+هذه الساعة الليلةاسمها volumetric flux في عندنا
+
+173
+00:13:21,350 --> 00:13:26,170
+دروبر، الدروبر هادي باخد فيه حجوم عشوائية في عندنا
+
+174
+00:13:26,170 --> 00:13:31,110
+balance ميزان أكيد بوزن فيه في عندنا سيلندر، هاد
+
+175
+00:13:31,110 --> 00:13:36,820
+عبارة عن أنبوب استواني مدرج باخد فيه حجمفي عندنا
+
+176
+00:13:36,820 --> 00:13:40,860
+funnel محجن زجاجي بينحط فيه حاجة بنسميها filter
+
+177
+00:13:40,860 --> 00:13:44,340
+paper اللي هي للفلترة بنستخدمهم ال funnel و ال
+
+178
+00:13:44,340 --> 00:13:48,940
+filter paper محجن زجاجي بستخدم فيه ورقة للفلترة
+
+179
+00:13:48,940 --> 00:13:53,880
+ورقة مسامية بتسمح بنزول السوائل دون الشوائب
+
+180
+00:13:53,880 --> 00:14:00,020
+بنسميها filter paperهنا كمان test tube brush اللى
+
+181
+00:14:00,020 --> 00:14:03,460
+هى الفرشاية اللى بنظف فيها القنابيب بعد ما اخلص من
+
+182
+00:14:03,460 --> 00:14:07,680
+الشغل بتهى نظف القنبوب بداخل الفرشاية بنظفها فهى
+
+183
+00:14:07,680 --> 00:14:10,840
+ان حاجة بنسميها clamps اللى هو الملقط اللى بمسك
+
+184
+00:14:10,840 --> 00:14:14,880
+فيه القنابيب لما نسخنها فهى ان حاجة بنسميها rack
+
+185
+00:14:14,880 --> 00:14:19,560
+تحتوي على فتحات مناسبة للقنابيب بقعد فيه القنابيب
+
+186
+00:14:19,560 --> 00:14:25,410
+بحط القنابيب فيه بوقفهااجهزة تانية فيها جهاز
+
+187
+00:14:25,410 --> 00:14:27,830
+بنسميه centrifuge
+
diff --git a/PL9fwy3NUQKwZ1Q7zOg1ObgufPMjRI3WY1/wFi9BKypyvg.srt b/PL9fwy3NUQKwZ1Q7zOg1ObgufPMjRI3WY1/wFi9BKypyvg.srt
new file mode 100644
index 0000000000000000000000000000000000000000..39ea3be72ff566b08d671c868e0a92bf32d961d3
--- /dev/null
+++ b/PL9fwy3NUQKwZ1Q7zOg1ObgufPMjRI3WY1/wFi9BKypyvg.srt
@@ -0,0 +1,611 @@
+1
+00:00:00,000 --> 00:00:08,200
+في حلقة بتسميها aromatic amino acid  ميجي
+
+2
+00:00:08,200 --> 00:00:12,280
+نحكي عن حاجة اسمها ionization، ionization معناها
+
+3
+00:00:12,280 --> 00:00:17,340
+كيف يتصرف الحمض الأميني في الأوساط المختلفة، في 
+
+4
+00:00:17,340 --> 00:00:22,320
+وسط قاعدي، في وسط حمضي، في neutral pH، في وسط 
+
+5
+00:00:22,320 --> 00:00:26,840
+المتعادل لو جينا و تفرجنا على المعادلة هاي
+
+6
+00:00:26,840 --> 00:00:31,100
+اللي فوق في الوسط هذا حمض أميني موجود في وسط متعدد
+
+7
+00:00:31,100 --> 00:00:37,100
+الصيغة العامة لحمض أميني موجود في وسط متعدد بتكون
+
+8
+00:00:37,100 --> 00:00:41,760
+عدد الشحنات الموجبة بتساوي عدد الشحنات السالبة، إن
+
+9
+00:00:41,760 --> 00:00:45,020
+هنا مكتوبينه CH2 هذه الصيغة العامة لحمض الأميني
+
+10
+00:00:45,020 --> 00:00:49,400
+Central Carbon و Hydrogen و ال R group هي عبارة عن
+
+11
+00:00:49,400 --> 00:00:53,490
+ذرة الهيدروجين اللي محتبينها هنا، وانقدش 3 plus و 
+
+12
+00:00:53,490 --> 00:00:57,550
+COO minus هي الصيغة العامة للحمض الأميني في الوسط 
+
+13
+00:00:57,550 --> 00:01:01,610
+المتعادل، تكون الشحنات الموجبة بتساوي الشحنات 
+
+14
+00:01:01,610 --> 00:01:06,900
+السالبة، فالجهاد الـ net charge بتكون صفر، لو جيت 
+
+15
+00:01:06,900 --> 00:01:12,360
+أخدت هذا الحمض الأميني وحطيته في وسط حمضي، وسط حمضي
+
+16
+00:01:12,360 --> 00:01:19,600
+يعني جلالة الـ pH يعني أنه كان فيه زيادة في أيونات 
+
+17
+00:01:19,600 --> 00:01:23,440
+الهيدروجين، يعني هذا الوسط مليء بـ H plus زيادة
+
+18
+00:01:24,020 --> 00:01:27,960
+فالحمض الأميني هذا أخذته من المتعادل وحطيته في وسط 
+
+19
+00:01:27,960 --> 00:01:32,020
+الحمضي، وسط الحمضي فيه زيادة في أيونات الهيدروجين
+
+20
+00:01:32,020 --> 00:01:37,160
+الجاتمين بده يحتاج لهيدروجين، هياخد من الوسط COO-
+
+21
+00:01:37,160 --> 00:01:41,100
+محتاج لهيدروجين فبتستحب من وسط هيدروجين و بتتشبع 
+
+22
+00:01:41,100 --> 00:01:49,980
+وبتصير COOH فبتصير CH2 COOH NH3 بصير الشكل العام
+
+23
+00:01:49,980 --> 00:01:57,790
+له CH2 COOH NH3 Plus فأخد ذرّة هيدروجيني، صارت النت 
+
+24
+00:01:57,790 --> 00:02:01,770
+تتشارج عليه positive charge الموجود في المعادلة
+
+25
+00:02:01,770 --> 00:02:05,130
+على اليسار هذا اللي هو الصيغة العامة للحمض الأميني
+
+26
+00:02:05,130 --> 00:02:12,330
+في وسط حمضي CH2 COOH NH3 Plus إن الكربوكسيل أخدت
+
+27
+00:02:12,330 --> 00:02:16,870
+أيون هيدروجين أو بروتون هيدروجين من وسط الحمض اللي
+
+28
+00:02:16,870 --> 00:02:21,100
+هي فيه، فصارت COOH، الـ net charge في الوسط الحمضي 
+
+29
+00:02:21,100 --> 00:02:26,850
+صارت Positive charge، لو أخدت هاد الوسط من الوسط 
+
+30
+00:02:26,850 --> 00:02:31,490
+المتعادل وحطيته في وسط قاعدي، الوسط القاعدي مليء بـ
+
+31
+00:02:31,490 --> 00:02:39,130
+OH-، وسط مليء بـ OH-، OH- هي تحتاج إلى ذرّة هيدروجين
+
+32
+00:02:39,130 --> 00:02:45,270
+عشان تكون جزئنا، فبتروح على أي ذر جزئية تنزع منه
+
+33
+00:02:45,270 --> 00:02:49,470
+هيدروجين عشان تكوننا من عندنا، بنقدر ننزع منه
+
+34
+00:02:49,470 --> 00:02:55,360
+هيدروجين لاناتش 3 plus تاخد منه هيدروجين فبصير NH2 
+
+35
+00:02:55,360 --> 00:03:01,020
+COO minus هتتغير؟ لأ مش هتتغير، فالوسط المتعادل CH2
+
+36
+00:03:01,020 --> 00:03:05,280
+NH3 plus COO minus لما يروح على القاعدة اللي هي
+
+37
+00:03:05,280 --> 00:03:12,320
+على اليمين بيصير NH2 و COO minus، اتغيرت من الـ NH3
+
+38
+00:03:12,320 --> 00:03:16,070
+هنا نزعنا منها هيدروجين و صارت NH2، الـ net charge 
+
+39
+00:03:16,070 --> 00:03:21,490
+له بيكون minus، ففي حالة الوسط الحمضي .. في حالة 
+
+40
+00:03:21,490 --> 00:03:25,550
+وسط متعادل ال net charge بتكون zero، في حالة وسط
+
+41
+00:03:25,550 --> 00:03:29,710
+حمضي بيكون ال net charge positive، في حالة الوسط 
+
+42
+00:03:29,710 --> 00:03:35,070
+القاعدي ال net charge negative هلجد؟ الحمض الأميني
+
+43
+00:03:35,070 --> 00:03:38,830
+اللي بتكون متساوي عليه الشحنات الموجبة والشحنات
+
+44
+00:03:38,830 --> 00:03:42,930
+السالبة وال net charge بتكون بتساوي zero بسميه
+
+45
+00:03:42,930 --> 00:03:48,050
+Zwitterion، Zwitterion هو الحمض الأميني اللي تتساوي 
+
+46
+00:03:48,050 --> 00:03:52,350
+عليه الشحنة الموجبة والشحنة السالبة، يعني الشحنة
+
+47
+00:03:52,350 --> 00:03:57,150
+الكلية تكون صفر، وين موجود ال Zwitterion؟ في الوسط 
+
+48
+00:03:57,150 --> 00:04:01,490
+المتعادل، كان موجود بالوسط المتعادل، الحمض الأميني
+
+49
+00:04:01,490 --> 00:04:05,650
+بيكون الشحن الموجبة بتساوي الشحن السالبة، يعني الشحن
+
+50
+00:04:05,650 --> 00:04:10,790
+الكلية بتساوي صفر، وهذا بسميه Zwitterion، Zwitterion
+
+51
+00:04:10,790 --> 00:04:14,710
+هو الحمض الأميني الذي تتساوي عليه الشحن الموجبة 
+
+52
+00:04:14,710 --> 00:04:21,350
+والشحن السالبة، ونبدأ chart 0 عند الوسط المتعادل هذا، 
+
+53
+00:04:21,350 --> 00:04:24,090
+عند تكون الحمض الأميني اللي بتكون شحنات الموجبة 
+
+54
+00:04:24,090 --> 00:04:27,190
+السالبة بتساوي الموجبة، في حاجة بنسميها 
+
+55
+00:04:27,190 --> 00:04:32,730
+Isoelectric point أو الـpI، الـpI أو ال Isoelectric
+
+56
+00:04:32,730 --> 00:04:39,430
+point هي قيمة pH بتتساوى عند الشحن الموجبة والسالبة
+
+57
+00:04:39,430 --> 00:04:45,760
+على سطح الحمض الأميني، وبالتالي شحنة الكلية صفر، قيمة
+
+58
+00:04:45,760 --> 00:04:51,560
+pH تتساوى عندها الشحنة الموجبة والسالبة على سطح 
+
+59
+00:04:51,560 --> 00:04:57,020
+الحمض الأميني، وبالتالي شحنة الكلية بتساوي صفر، هاي 
+
+60
+00:04:57,020 --> 00:05:02,740
+الـ Isoelectric point، اختصارها pI تمام، في حاجة
+
+61
+00:05:02,740 --> 00:05:06,820
+بنسميها protonated معناها إنه اكتساب بروتون أو H
+
+62
+00:05:06,820 --> 00:05:11,140
+plus، في حاجة بنسميها Deprotonated يعني فقد بروتون 
+
+63
+00:05:11,970 --> 00:05:15,770
+اللي بياخد H plus، يعني في الوسط الحمضي COO minus
+
+64
+00:05:15,770 --> 00:05:20,230
+اكتسبت H، فهذه gain of البروتون يعني صارلها
+
+65
+00:05:20,230 --> 00:05:25,230
+protonated، Deprotonated يعني loss of البروتون، زي
+
+66
+00:05:25,230 --> 00:05:29,490
+الـ NH3 plus في الوسط القاعدي لما حضيتها صارت NH2
+
+67
+00:05:29,490 --> 00:05:34,050
+نزعت منها H، فبسميها بقول عنها أنا صارلها 
+
+68
+00:05:34,050 --> 00:05:37,450
+Deprotonated، فقدت البروتون
+
+69
+00:05:41,400 --> 00:05:45,340
+هنا بيحكيلي أن الـ Isoelectric point أو الـ PI هي 
+
+70
+00:05:45,340 --> 00:05:49,100
+قيمة pH اللي بيكون فيها الانيونيك بتساوي 
+
+71
+00:05:49,100 --> 00:05:55,060
+الكاتيونيك، ال net charge بتكون Zero، كل حمض أميني
+
+72
+00:05:55,060 --> 00:05:59,680
+له PI أو Isoelectric point بتختلف عن غيره حسب شو؟
+
+73
+00:05:59,680 --> 00:06:03,080
+حسب ال side chain، لأنه حاكي أن كل حمض أميني بيميزه 
+
+74
+00:06:03,080 --> 00:06:04,980
+عن غيره side chain
+
+75
+00:06:07,540 --> 00:06:12,180
+نحكي في الجزء العملي للمعملي الأول، جزء العملي
+
+76
+00:06:12,180 --> 00:06:16,540
+بيحكي عن qualitative test for amino acid، حوصات 
+
+77
+00:06:16,540 --> 00:06:21,560
+جودة للكشف عن الأحماض الأمينية، النتيجة عنها هتكون 
+
+78
+00:06:21,560 --> 00:06:27,440
+positive أو negative، ظهور لون أو عدم ظهور لون، في 
+
+79
+00:06:27,440 --> 00:06:30,300
+عندنا number of tests to detect the presence of
+
+80
+00:06:30,300 --> 00:06:33,440
+amino acid، هي دي تحوصات من الكشف عن الأحماض
+
+81
+00:06:33,440 --> 00:06:36,820
+الأمينية largely depend on the nature of side 
+
+82
+00:06:36,820 --> 00:06:40,900
+chain، علشان بيعتمد على ال side chain لأن بميز حمض
+
+83
+00:06:40,900 --> 00:06:45,430
+أمين عن الآخر هو ال side chain، الجهد احنا الفحوصات
+
+84
+00:06:45,430 --> 00:06:51,090
+اللي نشتغلها بيطلع معنا لون يتغير أو ما يتغير هذا
+
+85
+00:06:51,090 --> 00:06:56,560
+اللون له شدة، intensity of color، شدة اللون هي بتكون
+
+86
+00:06:56,560 --> 00:07:00,560
+تختلف، يعني ممكن هو يتلون، بس على درجات، من حاجة
+
+87
+00:07:00,560 --> 00:07:04,420
+للتاني تكون درجة بتختلف، شدة اللون هي علاش؟ بتعتمد،
+
+88
+00:07:04,420 --> 00:07:08,220
+بتعتمد على أول إشي الـnumber of reacting unit
+
+89
+00:07:08,220 --> 00:07:12,120
+number of reacting unit، اللي هي ال reacting unit
+
+90
+00:07:12,120 --> 00:07:16,320
+هي عبارة عن المادة الفعالة، قداش عدد المادة الفعالة
+
+91
+00:07:16,320 --> 00:07:19,760
+الموجودة؟ إيش هي المادة الفعالة؟ دي الاشي اللي
+
+92
+00:07:19,760 --> 00:07:23,780
+بدور عليها الفحص، أنا بشتغل فحص مثلا بيدور على
+
+93
+00:07:23,780 --> 00:07:26,820
+peptide bond، إذا موجودة ال peptide bond، يعطيني 
+
+94
+00:07:26,820 --> 00:07:30,760
+لون، إذا مش موجودة، مش هيتغير اللون، طب ال peptide
+
+95
+00:07:30,760 --> 00:07:34,020
+bond هذه ممكن تكون بس رد تتين، ويعطيني لون، ممكن 
+
+96
+00:07:34,020 --> 00:07:37,220
+تكون تلاتة ويعطيني لون، بس هل اللون هيكون نفسه إذا
+
+97
+00:07:37,220 --> 00:07:40,900
+كانوا تنتين وإذا كانوا تلاتة، شدة اللون هتختلف
+
+98
+00:07:40,900 --> 00:07:45,660
+بناء على number of reacting unit، الحاجة التانية
+
+99
+00:07:45,660 --> 00:07:48,720
+اللي بتعتمد عليها شدة اللون هي الـ accessibility 
+
+100
+00:07:48,720 --> 00:07:52,900
+to reaction، هل هذه المادة الفعالة الـ reacting unit
+
+101
+00:07:52,900 --> 00:07:57,200
+قريبة من التفاعل، هل هي حرة، free، بتقدر تيجي
+
+102
+00:07:57,200 --> 00:07:59,840
+ترتبط، تشتغل مع التفاعل، مع الـ agent اللي أنا
+
+103
+00:07:59,840 --> 00:08:03,780
+حطيته، تشتغل و تتغير اللون، ولا إنها مشغولة، مش
+
+104
+00:08:03,780 --> 00:08:07,780
+هتقدر في حواجز، مش هتقدر تيجي ترتبط، هي بتسميها 
+
+105
+00:08:07,780 --> 00:08:11,680
+accessibility to reaction، إذا تشدت اللون تعتمد على 
+
+106
+00:08:11,680 --> 00:08:15,300
+هشتان على ال number of reacting unit وعلى
+
+107
+00:08:15,300 --> 00:08:21,340
+accessibility to reaction، إيش 
+
+108
+00:08:21,340 --> 00:08:24,420
+هي الفحصات اللي هنستخدمها للكشف عن الأحماض
+
+109
+00:08:24,420 --> 00:08:29,380
+الأمينية؟ هي فحص اسمه ninhydrin test for free
+
+110
+00:08:29,380 --> 00:08:34,300
+alpha amino group، على Free Alpha Amino Group إذا
+
+111
+00:08:34,300 --> 00:08:38,020
+موجودة يتغير اللون وبيعطي visitant، إذا مش موجودة
+
+112
+00:08:38,020 --> 00:08:41,740
+مش هيتغير اللون، فال Free Alpha Amino Group هاي 
+
+113
+00:08:41,740 --> 00:08:45,880
+بسميها ال reacting unit، فحص التاني اسمه biuret
+
+114
+00:08:45,880 --> 00:08:50,100
+test، بتدور على ال peptide bond، لإنه فحص ال
+
+115
+00:08:50,100 --> 00:08:55,910
+xanthoproteic test، Xanthoproteic Test for Aromatic
+
+116
+00:08:55,910 --> 00:09:02,110
+Amino Acid، للأحماض الأمينية اللي تحتوي على حلقة بنزين نشطة
+
+117
+00:09:02,110 --> 00:09:06,170
+نضيف هنا Activated Aromatic Amino Acid، هنا فحص اسمه
+
+118
+00:09:11,560 --> 00:09:16,100
+Hopkins-Cole أو glycosilic acid reaction اللي هو اسمه
+
+119
+00:09:16,100 --> 00:09:20,280
+Hopkins-Cole، glycosilic acid reaction، بدور على 
+
+120
+00:09:20,280 --> 00:09:24,780
+الاندولي جروب، الـMillon’s test for amino acid
+
+121
+00:09:24,780 --> 00:09:29,260
+containing hydroxyl phenol group، للفينولي جروب، 
+
+122
+00:09:29,260 --> 00:09:33,050
+ال amino acid اللي بيحتوي على فينولي جروب، السلكيشي
+
+123
+00:09:33,050 --> 00:09:37,710
+test for ليجوانيديامين، لليجوانيديامين 
+
+124
+00:09:37,710 --> 00:09:42,570
+لسلفايت test بيدور على ال sulfur-containing group
+
+125
+00:09:42,570 --> 00:09:45,970
+للحمض الأميني اللي يحتوي على ال sulfur-containing
+
+126
+00:09:45,970 --> 00:09:49,610
+group، هو اللي هيعطيني positive غير هيك مش هيعطيني
+
+127
+00:09:49,610 --> 00:09:50,230
+positive
+
+129
+00:09:53,040 --> 00:09:56,300
+نجي نحكي عن أول فحص، أول فحص هو الـNinhydrin
+
+130
+00:09:56,300 --> 00:09:59,720
+الـNinhydrin، إيش الـobjective للفحص، إيش الهدف
+
+131
+00:09:59,720 --> 00:10:03,600
+للفحص؟ to detect the presence of free alpha amino
+
+132
+00:10:03,600 --> 00:10:07,820
+group، بيدور على الـfree alpha amino group، إذا
+
+133
+00:10:07,820 --> 00:10:11,880
+موجود free alpha amino group، هيتغير اللون، 
+
+134
+00:10:11,880 --> 00:10:15,200
+بيعطيني لون بارد، في حالات اللي موجودة في free
+
+135
+00:10:15,200 --> 00:10:18,810
+alpha amino group، مين عنده فيه Alpha Amino Group؟
+
+136
+00:10:18,810 --> 00:10:21,810
+إذا ذاكرين الحمض الأميني، عشان الصيغة العامة له
+
+137
+00:10:21,810 --> 00:10:25,250
+تكون من الـ Center Carbon و من الـ Hydrogen و من
+
+138
+00:10:25,250 --> 00:10:28,350
+الـ Side Chain و من Carboxyl Group و من Amino
+
+139
+00:10:28,350 --> 00:10:33,070
+Group، Amino Group هاي سميناها Primary Amino Group
+
+140
+00:10:33,070 --> 00:10:37,330
+أو Alpha Amino Group، معادة في حالة البرولين
+
+141
+00:10:37,330 --> 00:10:41,770
+سميناها Amino Group، يعني الأحماض الأمينية كلها
+
+142
+00:10:41,770 --> 00:10:47,190
+تحتوي على free alpha amino group ما عدا لبرولين
+
+143
+00:10:47,190 --> 00:10:51,030
+عنده amino group، يعني مين هيعطيني positive مع هذا
+
+144
+00:10:51,030 --> 00:10:54,130
+الفحص لو أنا اشتغلت مجموعة أحماض أمينية على هذا 
+
+145
+00:10:54,130 --> 00:10:57,570
+الفحص؟ كلهم هيعطوني positive لأنهم كلهم بيحتوي على 
+
+146
+00:10:57,570 --> 00:10:59,130
+free alpha amino group
+
+147
+00:11:02,330 --> 00:11:04,690
+بالنسبة للـ objective to detect the presence of
+
+148
+00:11:04,690 --> 00:11:07,150
+free alpha amino group، هل موجود free alpha amino
+
+149
+00:11:07,150 --> 00:11:10,710
+group أو لا، إذا تغير اللغة يعني موجود، إذا ما تغيرش
+
+150
+00:11:10,710 --> 00:11:13,950
+يعني مش موجود، طيب إيش ال principle مبتدأ العمل
+
+151
+00:11:13,950 --> 00:11:17,730
+للفحص، كيف يشتغل الفحص، ال principle، لو نعملها على
+
+152
+00:11:17,730 --> 00:11:21,330
+نظام معادلات بيسهل عليه، كيف هذا الفحص له ال 
+
+153
+00:11:21,330 --> 00:11:24,830
+principle أنه عندنا amino acid أنا بضيف حمض أميني 
+
+154
+00:11:24,830 --> 00:11:26,850
+بضيف عليه نهيدرين، رياكشن
diff --git a/PL9fwy3NUQKwZ1Q7zOg1ObgufPMjRI3WY1/wFi9BKypyvg_postprocess.srt b/PL9fwy3NUQKwZ1Q7zOg1ObgufPMjRI3WY1/wFi9BKypyvg_postprocess.srt
new file mode 100644
index 0000000000000000000000000000000000000000..f932c2d452e025385b85d907341786d23399e2a7
--- /dev/null
+++ b/PL9fwy3NUQKwZ1Q7zOg1ObgufPMjRI3WY1/wFi9BKypyvg_postprocess.srt
@@ -0,0 +1,616 @@
+1
+00:00:00,000 --> 00:00:08,200
+في حلقة بتسميها aromatic amino acid ميجي
+
+2
+00:00:08,200 --> 00:00:12,280
+نحكي عن حاجة اسمها ionization ionization معناها
+
+3
+00:00:12,280 --> 00:00:17,340
+كيف يتصرف الحمض الأميني في الأوساط المختلفة، في
+
+4
+00:00:17,340 --> 00:00:22,320
+وسط قاعدي، في وسط حمضي، في neutral pH، في وسط
+
+5
+00:00:22,320 --> 00:00:26,840
+المتعانيهلجيت لو جينا و متفرج على المعادلة هاي
+
+6
+00:00:26,840 --> 00:00:31,100
+اللي فوق في الوسط هذا حمد أميني موجود في وسط متعدد
+
+7
+00:00:31,100 --> 00:00:37,100
+الصيغة العامة لحمد أميني موجود في وسط متعدد بتكون
+
+8
+00:00:37,100 --> 00:00:41,760
+عدد الشحنات الموجبة بتساوي عدد الشحنات السالمة ان
+
+9
+00:00:41,760 --> 00:00:45,020
+هنا مكتوبينه CH2 هذه الصيغة العامة لحمد الأميني
+
+10
+00:00:45,020 --> 00:00:49,400
+Central Carbon و Hydrogen و ال R group هي عبارة عن
+
+11
+00:00:49,400 --> 00:00:53,490
+ذرة الهيدروجين اللي محتبينها هناوانقدش 3 plus و
+
+12
+00:00:53,490 --> 00:00:57,550
+COO minus هي الصيغة العامة للحمد الأميني في الوسط
+
+13
+00:00:57,550 --> 00:01:01,610
+المتعادي تكون الشحنات الموجبة بتساوي الشحنات
+
+14
+00:01:01,610 --> 00:01:06,900
+السالمةفالجهاد الـ net charge بتكون زيه لو جيت
+
+15
+00:01:06,900 --> 00:01:12,360
+أخدت هذا الحمض الأميني حاطيته في وسط حمضي وسط حمضي
+
+16
+00:01:12,360 --> 00:01:19,600
+يعني جلالة الـ pH يعني أنه كان فيه زيادة في أيونات
+
+17
+00:01:19,600 --> 00:01:23,440
+الهيدروجين يعني هذا الوسط مليء بـ H plus زيادة
+
+18
+00:01:24,020 --> 00:01:27,960
+فالحمض الأميني هذا خدته من المتعدل وحطيته في وسط
+
+19
+00:01:27,960 --> 00:01:32,020
+الحمضي وسط الحمضي فيه زيادة في أيونات الهيدروجين
+
+20
+00:01:32,020 --> 00:01:37,160
+الجاتمين بده يحتاج لهيدروجين هياخد من الوسط COO-
+
+21
+00:01:37,160 --> 00:01:41,100
+محتاج لهيدروجين فبتستحب من وسط هيدروجين و بتتشبع
+
+22
+00:01:41,100 --> 00:01:49,980
+وبتصير COOH فبتصير CH2 COOH NH3 بصير الشكل العام
+
+23
+00:01:49,980 --> 00:01:57,790
+لهCH2 COOH NH3 Plus فأخد ذرّة هيدروجيني صارت النت
+
+24
+00:01:57,790 --> 00:02:01,770
+تتشارج عليه positive charge الموجود في المعادلة
+
+25
+00:02:01,770 --> 00:02:05,130
+على اليسار هذا اللي هو الصيغة العامة للحمض الأميني
+
+26
+00:02:05,130 --> 00:02:12,330
+في وسط حمضي CH2 COOH NH3 Plus ان الكربوكسيل أخدت
+
+27
+00:02:12,330 --> 00:02:16,870
+أيون هيدروجين أو بروتون هيدروجين من وسط الحمض اللي
+
+28
+00:02:16,870 --> 00:02:21,100
+هي فيه فصارت COOHالـ net charge في الوسط الحمدي
+
+29
+00:02:21,100 --> 00:02:26,850
+صارتPositive charge لو أخدت هاد الوسط من الوسط
+
+30
+00:02:26,850 --> 00:02:31,490
+المتعادل وحطته في وسط قاعده الوسط القاعد مليء بـ
+
+31
+00:02:31,490 --> 00:02:39,130
+OH- وسط مليء بـ OH- OH- هي تحتاج إلى ذرّة هيدروجين
+
+32
+00:02:39,130 --> 00:02:45,270
+عشان تكون جزئنا فبتروح على أي ذر جزية تنزع منه
+
+33
+00:02:45,270 --> 00:02:49,470
+هيدروجين عشان تكوننا من عندنا بنقدر ننزع منه
+
+34
+00:02:49,470 --> 00:02:55,360
+هيدروجين لاناتش 3 plusتاخد منه هيدروجين فبصير NH2
+
+35
+00:02:55,360 --> 00:03:01,020
+COO minus هتتغير؟ لأ مش هتتغير فالوسط المتعدل CH2
+
+36
+00:03:01,020 --> 00:03:05,280
+NH3 plus COO minus لما يروح على القاعدة اللي هي
+
+37
+00:03:05,280 --> 00:03:12,320
+على اليمين بيصير NH2 و COO minus اتغيرت من الـ NH3
+
+38
+00:03:12,320 --> 00:03:16,070
+هنا زعلنا منها هيدروجين و صارت NH2الـ net charge
+
+39
+00:03:16,070 --> 00:03:21,490
+له بيكون minus ففي حالة الوسط الحمضي .. في حالة
+
+40
+00:03:21,490 --> 00:03:25,550
+وسط متعادل ال net charge بتكون zero في حالة وسط
+
+41
+00:03:25,550 --> 00:03:29,710
+حمضي بيكون ال net charge positive في حالة الوسط
+
+42
+00:03:29,710 --> 00:03:35,070
+القاعدي ال net charge negativeهلجد؟ الحمض الأميني
+
+43
+00:03:35,070 --> 00:03:38,830
+اللي بتكون متساوي عليه الشحنات الموجبة والشحنات
+
+44
+00:03:38,830 --> 00:03:42,930
+السالبة وال net charge بتكون بتساوي zero بسميه
+
+45
+00:03:42,930 --> 00:03:48,050
+Zwitterion Zwitterion هو الحمض الأميني اللي تتساوي
+
+46
+00:03:48,050 --> 00:03:52,350
+عليه الشحنة الموجبة والشحنة السالبة يعني الشحنة
+
+47
+00:03:52,350 --> 00:03:57,150
+الكلية تكون سفات وين موجود ال Zwitterion؟ في الوسط
+
+48
+00:03:57,150 --> 00:04:01,490
+المتعادل كان موجودبالوسط المتعدل الحمض الأميني
+
+49
+00:04:01,490 --> 00:04:05,650
+بيكون الشحن الموجبة بتساوي الشحن السالي يعني الشحن
+
+50
+00:04:05,650 --> 00:04:10,790
+الكلية بتساوي صفر وهذا بسميه Zwitterion Zwitterion
+
+51
+00:04:10,790 --> 00:04:14,710
+هو الحمض الأميني الذي تتساوي عليه الشحن الموجبة
+
+52
+00:04:14,710 --> 00:04:21,350
+والشحن السالي ونبدأ chart 0عند الوسط المتعادل هذا،
+
+53
+00:04:21,350 --> 00:04:24,090
+عند تكون الحمض الأميني اللي بتكون شحنات الموجبة
+
+54
+00:04:24,090 --> 00:04:27,190
+السالبة بتساوي الموجبة، في حاجة بنسميها
+
+55
+00:04:27,190 --> 00:04:32,730
+ISOelectric bond أو الـBI الـBI أو ال ISOelectric
+
+56
+00:04:32,730 --> 00:04:39,430
+bond هي قيمة BH بتتساوى عند الشحن الموجبة والسالبة
+
+57
+00:04:39,430 --> 00:04:45,760
+على سطح الحمض الأمينيوبالتالي شحنة الكلية سفن قيمة
+
+58
+00:04:45,760 --> 00:04:51,560
+BH تتساوى عندها الشحنة الموجبة والسالبة على سطح
+
+59
+00:04:51,560 --> 00:04:57,020
+الحمض الأمين وبالتالي شحنة الكلية بتساوي سفن هاي
+
+60
+00:04:57,020 --> 00:05:02,740
+الـ Isoelectric point الاكتصارة BI تمام، في حاجة
+
+61
+00:05:02,740 --> 00:05:06,820
+بنسميها protonated معناها إنه اكتساب بروتون أو H
+
+62
+00:05:06,820 --> 00:05:11,140
+plus في حاجة بنسميها Deprotonated يعني فقد بروتون
+
+63
+00:05:11,970 --> 00:05:15,770
+اللي بياخد H plus يعني في الوسط الحمضي COO minus
+
+64
+00:05:15,770 --> 00:05:20,230
+اكتسبت H فهذه gain of البروتون يعني صارلها
+
+65
+00:05:20,230 --> 00:05:25,230
+protonated Deprotonated يعني loss of البروتون زي
+
+66
+00:05:25,230 --> 00:05:29,490
+الـ NH3 plus في الوسط القاعدي لما حضيتها صارت NH2
+
+67
+00:05:29,490 --> 00:05:34,050
+نزعت منها H فبسميها بقول عنها أنا صارلها
+
+68
+00:05:34,050 --> 00:05:37,450
+Deprotonated فقدت البروتون
+
+69
+00:05:41,400 --> 00:05:45,340
+هنا بيحكيلي أن الـ Isoelectric point أو الـ PI هي
+
+70
+00:05:45,340 --> 00:05:49,100
+قيمة BH اللي بيكون فيها الانيونيك بتساوي
+
+71
+00:05:49,100 --> 00:05:55,060
+الكاتيونيك ال net charge بتكون Zero كل حمض أميني
+
+72
+00:05:55,060 --> 00:05:59,680
+له PI أو Isoelectric point بتختلف عن غيره حسب شو؟
+
+73
+00:05:59,680 --> 00:06:03,080
+حسب ال side chain لأنه حاكي أن كل حمض أميني بيميزه
+
+74
+00:06:03,080 --> 00:06:04,980
+عن غيره side chain
+
+75
+00:06:07,540 --> 00:06:12,180
+نحكي في الجزء العملي للمعملي الأول جزء العملي
+
+76
+00:06:12,180 --> 00:06:16,540
+بيحكي عن qualitative test for amino acid حوصات
+
+77
+00:06:16,540 --> 00:06:21,560
+جودة لالكشف عن الأحماض الأمينية النتيجة عنها هتكون
+
+78
+00:06:21,560 --> 00:06:27,440
+positive أو negativeظهور لون أو عدم ظهور لون في
+
+79
+00:06:27,440 --> 00:06:30,300
+عندنا number of tests to detect the presence of
+
+80
+00:06:30,300 --> 00:06:33,440
+amino acid هي دي تحوصات من الكشف عن الأحمد
+
+81
+00:06:33,440 --> 00:06:36,820
+الأمينية largely depend on the nature of side
+
+82
+00:06:36,820 --> 00:06:40,900
+chain علشان بيعتمد على ال side chain لأن بميز حمد
+
+83
+00:06:40,900 --> 00:06:45,430
+أمين عن الآخر هو ال side chainالجهد احنا الفحوصات
+
+84
+00:06:45,430 --> 00:06:51,090
+اللي نشتغلها بيطلع معنا لون يتغير او ما يتغير هذا
+
+85
+00:06:51,090 --> 00:06:56,560
+اللون له شدة intensity of colorشدة اللون هي بتكون
+
+86
+00:06:56,560 --> 00:07:00,560
+تختلف، يعني ممكن هو يتلون، بس على درجات، من حاجة
+
+87
+00:07:00,560 --> 00:07:04,420
+للتاني تكون درجة بتختلف شدة اللون هي علاش؟ بتعتمد،
+
+88
+00:07:04,420 --> 00:07:08,220
+بتعتمد على أول إشي الـnumber of reacting unit
+
+89
+00:07:08,220 --> 00:07:12,120
+number of reacting unit، اللي هي ال reacting unit
+
+90
+00:07:12,120 --> 00:07:16,320
+هي عبارة عن المادة الفعالةقداش عدد المادة الفعالة
+
+91
+00:07:16,320 --> 00:07:19,760
+الموجودة؟ إيش هي المادة الفعالة؟ دي الاشي اللي
+
+92
+00:07:19,760 --> 00:07:23,780
+بدور عليها الفحص أنا بشتغل فحص مثلا بيدور على
+
+93
+00:07:23,780 --> 00:07:26,820
+peptide bond إذا موجودة ال peptide bond، يعطيني
+
+94
+00:07:26,820 --> 00:07:30,760
+لون إذا مش موجودة، مش هيتغير اللون طب ال peptide
+
+95
+00:07:30,760 --> 00:07:34,020
+bond هذه ممكن تكون بس رد تتين، ويعطيني لون، ممكن
+
+96
+00:07:34,020 --> 00:07:37,220
+تكون تلاتة ويعطيني لون، بس هل اللون هيكون نفسه إذا
+
+97
+00:07:37,220 --> 00:07:40,900
+كانوا تنتين وإذا كانوا تلاتة، شدة اللون هتختلف
+
+98
+00:07:40,900 --> 00:07:45,660
+بناء على number of reacting unitالحاجة التانية
+
+99
+00:07:45,660 --> 00:07:48,720
+اللي بتعتمد عليها شدة اللون هي الـ accessibility
+
+100
+00:07:48,720 --> 00:07:52,900
+to reaction هل هذه المادة الفعالة الـ acting unit
+
+101
+00:07:52,900 --> 00:07:57,200
+قريبة من التفاعل، هل هي حرة، free، بتقدر تيجي
+
+102
+00:07:57,200 --> 00:07:59,840
+ترتبط، تشتغل مع التفاعل، مع الـ agent اللي أنا
+
+103
+00:07:59,840 --> 00:08:03,780
+حطيته، تشتغل و تتغير اللون، ولا إنها مشغولة، مش
+
+104
+00:08:03,780 --> 00:08:07,780
+هتقدر في حواجز، مش هتقدر تيجي ترتبطهي بتسميها
+
+105
+00:08:07,780 --> 00:08:11,680
+accessibility to reaction إذا تشدت اللون تعتمد على
+
+106
+00:08:11,680 --> 00:08:15,300
+هشتان على ال number of reacting unit وعلى
+
+107
+00:08:15,300 --> 00:08:21,340
+accessibility to reaction إيش
+
+108
+00:08:21,340 --> 00:08:24,420
+هي الفحصات اللي هنستخدمها للكشف عن الأحماض
+
+109
+00:08:24,420 --> 00:08:29,380
+الأمينية؟ هي فحص اسمه ninhydrin test for free
+
+110
+00:08:29,380 --> 00:08:34,300
+alpha amino groupعلى Free Alpha Amino Group إذا
+
+111
+00:08:34,300 --> 00:08:38,020
+موجودة يتغير اللون وبيعطي visitant إذا مش موجودة
+
+112
+00:08:38,020 --> 00:08:41,740
+مش هيتغير اللون فال Free Alpha Amino Group هاي
+
+113
+00:08:41,740 --> 00:08:45,880
+بسميها ال reacting unit فحص التاني اسمه biuret
+
+114
+00:08:45,880 --> 00:08:50,100
+test بتدور على ال peptide bond لإنه فحص ال
+
+115
+00:08:50,100 --> 00:08:55,910
+xanthoprotect testZanto Proteic Test for Aromatic
+
+116
+00:08:55,910 --> 00:09:02,110
+Amino Acid لأ
+
+117
+00:09:02,110 --> 00:09:06,170
+الأحماض الأمنية اللي تحتوي على حلقة بنزين نشطة
+
+118
+00:09:06,170 --> 00:09:11,560
+نضيف هنا Activated Aromatic Amino Acidهنا فحص اسمه
+
+119
+00:09:11,560 --> 00:09:16,100
+hopkincol أو glycosilic acid reaction اللي هو اسمه
+
+120
+00:09:16,100 --> 00:09:20,280
+hopkincol، glycosilic acid reaction، بدور على
+
+121
+00:09:20,280 --> 00:09:24,780
+الاندولي جروب الـmelons test for amino acid
+
+122
+00:09:24,780 --> 00:09:29,260
+containing hydroxyl phenol group، للفينولي جروب،
+
+123
+00:09:29,260 --> 00:09:33,050
+ال amino acid اللي بيحتوي على فينولي جروبالسكيكوشي
+
+124
+00:09:33,050 --> 00:09:37,710
+تاست for ليجوانيدياميجرو لليجوانيدياميجرو
+
+125
+00:09:37,710 --> 00:09:42,570
+لتسالفايت تاست بيدور على ال self-hydraulic group
+
+126
+00:09:42,570 --> 00:09:45,970
+للحمض الأمين اللي يحتوي على ال self-hydraulic
+
+127
+00:09:45,970 --> 00:09:49,610
+group هو اللي هيعطيني positive غير هيك مش هيعطيني
+
+128
+00:09:49,610 --> 00:09:50,230
+positive
+
+129
+00:09:53,040 --> 00:09:56,300
+نجي نحكي عن أول فحص، أول فحص هو الـNilhedrine
+
+130
+00:09:56,300 --> 00:09:59,720
+الـNilhedrine، إيش الـobjective للفحص، إيش الهدف
+
+131
+00:09:59,720 --> 00:10:03,600
+للفحص؟ to detect the presence of free alpha amino
+
+132
+00:10:03,600 --> 00:10:07,820
+group، بيدور على الـfree alpha amino group، إذا
+
+133
+00:10:07,820 --> 00:10:11,880
+موجود free alpha amino group، هيتغير اللون،
+
+134
+00:10:11,880 --> 00:10:15,200
+بيعطيني لون بارد، في حالات اللي موجودة في free
+
+135
+00:10:15,200 --> 00:10:18,810
+alpha amino groupمين عنده فيه Alpha Amino Group؟
+
+136
+00:10:18,810 --> 00:10:21,810
+إذا ذاكرين الحمض الأميني، عشان الصيغة العامة له
+
+137
+00:10:21,810 --> 00:10:25,250
+تكون من الـ Center Carbon و من الـ Hydrogenة و من
+
+138
+00:10:25,250 --> 00:10:28,350
+الـ Side Chain و من Carboxyl Group و من Amino
+
+139
+00:10:28,350 --> 00:10:33,070
+Group Amino Group هاي سميناها Primary Amino Group
+
+140
+00:10:33,070 --> 00:10:37,330
+أو Alpha Amino Group معادة في حالة البرولين
+
+141
+00:10:37,330 --> 00:10:41,770
+سميناها Amino Groupيعني الأحماض الأمينية كلها
+
+142
+00:10:41,770 --> 00:10:47,190
+تحتوي على free alpha amino group ما عدا لبرولين
+
+143
+00:10:47,190 --> 00:10:51,030
+عنده amino group يعني مين هيعطيني positive مع هذا
+
+144
+00:10:51,030 --> 00:10:54,130
+الفحص لو أنا اشتغلت مجموعة أحماض أمينية على هذا
+
+145
+00:10:54,130 --> 00:10:57,570
+الفحص؟ كلهم هيعطوني positive لأنهم كلهم بيحتوي على
+
+146
+00:10:57,570 --> 00:10:59,130
+free alpha amino group
+
+147
+00:11:02,330 --> 00:11:04,690
+بالنسبة للـ objective to detect the presence of
+
+148
+00:11:04,690 --> 00:11:07,150
+free alpha amino group هل موجود free alpha amino
+
+149
+00:11:07,150 --> 00:11:10,710
+group أو لا إذا تغير اللغة يعني موجود إذا ما تغيرش
+
+150
+00:11:10,710 --> 00:11:13,950
+يعني مش موجود طيب إيش ال principle مبتدأ العمل
+
+151
+00:11:13,950 --> 00:11:17,730
+للفحص كيف يشتغل الفحص ال principle لو نعملها على
+
+152
+00:11:17,730 --> 00:11:21,330
+نظام معادلات بيسهل عليه كيف هذا الفحص له ال
+
+153
+00:11:21,330 --> 00:11:24,830
+principle أنه عندنا amino acid أنا بضيف حمض أميني
+
+154
+00:11:24,830 --> 00:11:26,850
+بضيف عليه نهيدرينرياشن
+
diff --git a/PL9fwy3NUQKwZ1Q7zOg1ObgufPMjRI3WY1/wFi9BKypyvg_raw.srt b/PL9fwy3NUQKwZ1Q7zOg1ObgufPMjRI3WY1/wFi9BKypyvg_raw.srt
new file mode 100644
index 0000000000000000000000000000000000000000..f932c2d452e025385b85d907341786d23399e2a7
--- /dev/null
+++ b/PL9fwy3NUQKwZ1Q7zOg1ObgufPMjRI3WY1/wFi9BKypyvg_raw.srt
@@ -0,0 +1,616 @@
+1
+00:00:00,000 --> 00:00:08,200
+في حلقة بتسميها aromatic amino acid ميجي
+
+2
+00:00:08,200 --> 00:00:12,280
+نحكي عن حاجة اسمها ionization ionization معناها
+
+3
+00:00:12,280 --> 00:00:17,340
+كيف يتصرف الحمض الأميني في الأوساط المختلفة، في
+
+4
+00:00:17,340 --> 00:00:22,320
+وسط قاعدي، في وسط حمضي، في neutral pH، في وسط
+
+5
+00:00:22,320 --> 00:00:26,840
+المتعانيهلجيت لو جينا و متفرج على المعادلة هاي
+
+6
+00:00:26,840 --> 00:00:31,100
+اللي فوق في الوسط هذا حمد أميني موجود في وسط متعدد
+
+7
+00:00:31,100 --> 00:00:37,100
+الصيغة العامة لحمد أميني موجود في وسط متعدد بتكون
+
+8
+00:00:37,100 --> 00:00:41,760
+عدد الشحنات الموجبة بتساوي عدد الشحنات السالمة ان
+
+9
+00:00:41,760 --> 00:00:45,020
+هنا مكتوبينه CH2 هذه الصيغة العامة لحمد الأميني
+
+10
+00:00:45,020 --> 00:00:49,400
+Central Carbon و Hydrogen و ال R group هي عبارة عن
+
+11
+00:00:49,400 --> 00:00:53,490
+ذرة الهيدروجين اللي محتبينها هناوانقدش 3 plus و
+
+12
+00:00:53,490 --> 00:00:57,550
+COO minus هي الصيغة العامة للحمد الأميني في الوسط
+
+13
+00:00:57,550 --> 00:01:01,610
+المتعادي تكون الشحنات الموجبة بتساوي الشحنات
+
+14
+00:01:01,610 --> 00:01:06,900
+السالمةفالجهاد الـ net charge بتكون زيه لو جيت
+
+15
+00:01:06,900 --> 00:01:12,360
+أخدت هذا الحمض الأميني حاطيته في وسط حمضي وسط حمضي
+
+16
+00:01:12,360 --> 00:01:19,600
+يعني جلالة الـ pH يعني أنه كان فيه زيادة في أيونات
+
+17
+00:01:19,600 --> 00:01:23,440
+الهيدروجين يعني هذا الوسط مليء بـ H plus زيادة
+
+18
+00:01:24,020 --> 00:01:27,960
+فالحمض الأميني هذا خدته من المتعدل وحطيته في وسط
+
+19
+00:01:27,960 --> 00:01:32,020
+الحمضي وسط الحمضي فيه زيادة في أيونات الهيدروجين
+
+20
+00:01:32,020 --> 00:01:37,160
+الجاتمين بده يحتاج لهيدروجين هياخد من الوسط COO-
+
+21
+00:01:37,160 --> 00:01:41,100
+محتاج لهيدروجين فبتستحب من وسط هيدروجين و بتتشبع
+
+22
+00:01:41,100 --> 00:01:49,980
+وبتصير COOH فبتصير CH2 COOH NH3 بصير الشكل العام
+
+23
+00:01:49,980 --> 00:01:57,790
+لهCH2 COOH NH3 Plus فأخد ذرّة هيدروجيني صارت النت
+
+24
+00:01:57,790 --> 00:02:01,770
+تتشارج عليه positive charge الموجود في المعادلة
+
+25
+00:02:01,770 --> 00:02:05,130
+على اليسار هذا اللي هو الصيغة العامة للحمض الأميني
+
+26
+00:02:05,130 --> 00:02:12,330
+في وسط حمضي CH2 COOH NH3 Plus ان الكربوكسيل أخدت
+
+27
+00:02:12,330 --> 00:02:16,870
+أيون هيدروجين أو بروتون هيدروجين من وسط الحمض اللي
+
+28
+00:02:16,870 --> 00:02:21,100
+هي فيه فصارت COOHالـ net charge في الوسط الحمدي
+
+29
+00:02:21,100 --> 00:02:26,850
+صارتPositive charge لو أخدت هاد الوسط من الوسط
+
+30
+00:02:26,850 --> 00:02:31,490
+المتعادل وحطته في وسط قاعده الوسط القاعد مليء بـ
+
+31
+00:02:31,490 --> 00:02:39,130
+OH- وسط مليء بـ OH- OH- هي تحتاج إلى ذرّة هيدروجين
+
+32
+00:02:39,130 --> 00:02:45,270
+عشان تكون جزئنا فبتروح على أي ذر جزية تنزع منه
+
+33
+00:02:45,270 --> 00:02:49,470
+هيدروجين عشان تكوننا من عندنا بنقدر ننزع منه
+
+34
+00:02:49,470 --> 00:02:55,360
+هيدروجين لاناتش 3 plusتاخد منه هيدروجين فبصير NH2
+
+35
+00:02:55,360 --> 00:03:01,020
+COO minus هتتغير؟ لأ مش هتتغير فالوسط المتعدل CH2
+
+36
+00:03:01,020 --> 00:03:05,280
+NH3 plus COO minus لما يروح على القاعدة اللي هي
+
+37
+00:03:05,280 --> 00:03:12,320
+على اليمين بيصير NH2 و COO minus اتغيرت من الـ NH3
+
+38
+00:03:12,320 --> 00:03:16,070
+هنا زعلنا منها هيدروجين و صارت NH2الـ net charge
+
+39
+00:03:16,070 --> 00:03:21,490
+له بيكون minus ففي حالة الوسط الحمضي .. في حالة
+
+40
+00:03:21,490 --> 00:03:25,550
+وسط متعادل ال net charge بتكون zero في حالة وسط
+
+41
+00:03:25,550 --> 00:03:29,710
+حمضي بيكون ال net charge positive في حالة الوسط
+
+42
+00:03:29,710 --> 00:03:35,070
+القاعدي ال net charge negativeهلجد؟ الحمض الأميني
+
+43
+00:03:35,070 --> 00:03:38,830
+اللي بتكون متساوي عليه الشحنات الموجبة والشحنات
+
+44
+00:03:38,830 --> 00:03:42,930
+السالبة وال net charge بتكون بتساوي zero بسميه
+
+45
+00:03:42,930 --> 00:03:48,050
+Zwitterion Zwitterion هو الحمض الأميني اللي تتساوي
+
+46
+00:03:48,050 --> 00:03:52,350
+عليه الشحنة الموجبة والشحنة السالبة يعني الشحنة
+
+47
+00:03:52,350 --> 00:03:57,150
+الكلية تكون سفات وين موجود ال Zwitterion؟ في الوسط
+
+48
+00:03:57,150 --> 00:04:01,490
+المتعادل كان موجودبالوسط المتعدل الحمض الأميني
+
+49
+00:04:01,490 --> 00:04:05,650
+بيكون الشحن الموجبة بتساوي الشحن السالي يعني الشحن
+
+50
+00:04:05,650 --> 00:04:10,790
+الكلية بتساوي صفر وهذا بسميه Zwitterion Zwitterion
+
+51
+00:04:10,790 --> 00:04:14,710
+هو الحمض الأميني الذي تتساوي عليه الشحن الموجبة
+
+52
+00:04:14,710 --> 00:04:21,350
+والشحن السالي ونبدأ chart 0عند الوسط المتعادل هذا،
+
+53
+00:04:21,350 --> 00:04:24,090
+عند تكون الحمض الأميني اللي بتكون شحنات الموجبة
+
+54
+00:04:24,090 --> 00:04:27,190
+السالبة بتساوي الموجبة، في حاجة بنسميها
+
+55
+00:04:27,190 --> 00:04:32,730
+ISOelectric bond أو الـBI الـBI أو ال ISOelectric
+
+56
+00:04:32,730 --> 00:04:39,430
+bond هي قيمة BH بتتساوى عند الشحن الموجبة والسالبة
+
+57
+00:04:39,430 --> 00:04:45,760
+على سطح الحمض الأمينيوبالتالي شحنة الكلية سفن قيمة
+
+58
+00:04:45,760 --> 00:04:51,560
+BH تتساوى عندها الشحنة الموجبة والسالبة على سطح
+
+59
+00:04:51,560 --> 00:04:57,020
+الحمض الأمين وبالتالي شحنة الكلية بتساوي سفن هاي
+
+60
+00:04:57,020 --> 00:05:02,740
+الـ Isoelectric point الاكتصارة BI تمام، في حاجة
+
+61
+00:05:02,740 --> 00:05:06,820
+بنسميها protonated معناها إنه اكتساب بروتون أو H
+
+62
+00:05:06,820 --> 00:05:11,140
+plus في حاجة بنسميها Deprotonated يعني فقد بروتون
+
+63
+00:05:11,970 --> 00:05:15,770
+اللي بياخد H plus يعني في الوسط الحمضي COO minus
+
+64
+00:05:15,770 --> 00:05:20,230
+اكتسبت H فهذه gain of البروتون يعني صارلها
+
+65
+00:05:20,230 --> 00:05:25,230
+protonated Deprotonated يعني loss of البروتون زي
+
+66
+00:05:25,230 --> 00:05:29,490
+الـ NH3 plus في الوسط القاعدي لما حضيتها صارت NH2
+
+67
+00:05:29,490 --> 00:05:34,050
+نزعت منها H فبسميها بقول عنها أنا صارلها
+
+68
+00:05:34,050 --> 00:05:37,450
+Deprotonated فقدت البروتون
+
+69
+00:05:41,400 --> 00:05:45,340
+هنا بيحكيلي أن الـ Isoelectric point أو الـ PI هي
+
+70
+00:05:45,340 --> 00:05:49,100
+قيمة BH اللي بيكون فيها الانيونيك بتساوي
+
+71
+00:05:49,100 --> 00:05:55,060
+الكاتيونيك ال net charge بتكون Zero كل حمض أميني
+
+72
+00:05:55,060 --> 00:05:59,680
+له PI أو Isoelectric point بتختلف عن غيره حسب شو؟
+
+73
+00:05:59,680 --> 00:06:03,080
+حسب ال side chain لأنه حاكي أن كل حمض أميني بيميزه
+
+74
+00:06:03,080 --> 00:06:04,980
+عن غيره side chain
+
+75
+00:06:07,540 --> 00:06:12,180
+نحكي في الجزء العملي للمعملي الأول جزء العملي
+
+76
+00:06:12,180 --> 00:06:16,540
+بيحكي عن qualitative test for amino acid حوصات
+
+77
+00:06:16,540 --> 00:06:21,560
+جودة لالكشف عن الأحماض الأمينية النتيجة عنها هتكون
+
+78
+00:06:21,560 --> 00:06:27,440
+positive أو negativeظهور لون أو عدم ظهور لون في
+
+79
+00:06:27,440 --> 00:06:30,300
+عندنا number of tests to detect the presence of
+
+80
+00:06:30,300 --> 00:06:33,440
+amino acid هي دي تحوصات من الكشف عن الأحمد
+
+81
+00:06:33,440 --> 00:06:36,820
+الأمينية largely depend on the nature of side
+
+82
+00:06:36,820 --> 00:06:40,900
+chain علشان بيعتمد على ال side chain لأن بميز حمد
+
+83
+00:06:40,900 --> 00:06:45,430
+أمين عن الآخر هو ال side chainالجهد احنا الفحوصات
+
+84
+00:06:45,430 --> 00:06:51,090
+اللي نشتغلها بيطلع معنا لون يتغير او ما يتغير هذا
+
+85
+00:06:51,090 --> 00:06:56,560
+اللون له شدة intensity of colorشدة اللون هي بتكون
+
+86
+00:06:56,560 --> 00:07:00,560
+تختلف، يعني ممكن هو يتلون، بس على درجات، من حاجة
+
+87
+00:07:00,560 --> 00:07:04,420
+للتاني تكون درجة بتختلف شدة اللون هي علاش؟ بتعتمد،
+
+88
+00:07:04,420 --> 00:07:08,220
+بتعتمد على أول إشي الـnumber of reacting unit
+
+89
+00:07:08,220 --> 00:07:12,120
+number of reacting unit، اللي هي ال reacting unit
+
+90
+00:07:12,120 --> 00:07:16,320
+هي عبارة عن المادة الفعالةقداش عدد المادة الفعالة
+
+91
+00:07:16,320 --> 00:07:19,760
+الموجودة؟ إيش هي المادة الفعالة؟ دي الاشي اللي
+
+92
+00:07:19,760 --> 00:07:23,780
+بدور عليها الفحص أنا بشتغل فحص مثلا بيدور على
+
+93
+00:07:23,780 --> 00:07:26,820
+peptide bond إذا موجودة ال peptide bond، يعطيني
+
+94
+00:07:26,820 --> 00:07:30,760
+لون إذا مش موجودة، مش هيتغير اللون طب ال peptide
+
+95
+00:07:30,760 --> 00:07:34,020
+bond هذه ممكن تكون بس رد تتين، ويعطيني لون، ممكن
+
+96
+00:07:34,020 --> 00:07:37,220
+تكون تلاتة ويعطيني لون، بس هل اللون هيكون نفسه إذا
+
+97
+00:07:37,220 --> 00:07:40,900
+كانوا تنتين وإذا كانوا تلاتة، شدة اللون هتختلف
+
+98
+00:07:40,900 --> 00:07:45,660
+بناء على number of reacting unitالحاجة التانية
+
+99
+00:07:45,660 --> 00:07:48,720
+اللي بتعتمد عليها شدة اللون هي الـ accessibility
+
+100
+00:07:48,720 --> 00:07:52,900
+to reaction هل هذه المادة الفعالة الـ acting unit
+
+101
+00:07:52,900 --> 00:07:57,200
+قريبة من التفاعل، هل هي حرة، free، بتقدر تيجي
+
+102
+00:07:57,200 --> 00:07:59,840
+ترتبط، تشتغل مع التفاعل، مع الـ agent اللي أنا
+
+103
+00:07:59,840 --> 00:08:03,780
+حطيته، تشتغل و تتغير اللون، ولا إنها مشغولة، مش
+
+104
+00:08:03,780 --> 00:08:07,780
+هتقدر في حواجز، مش هتقدر تيجي ترتبطهي بتسميها
+
+105
+00:08:07,780 --> 00:08:11,680
+accessibility to reaction إذا تشدت اللون تعتمد على
+
+106
+00:08:11,680 --> 00:08:15,300
+هشتان على ال number of reacting unit وعلى
+
+107
+00:08:15,300 --> 00:08:21,340
+accessibility to reaction إيش
+
+108
+00:08:21,340 --> 00:08:24,420
+هي الفحصات اللي هنستخدمها للكشف عن الأحماض
+
+109
+00:08:24,420 --> 00:08:29,380
+الأمينية؟ هي فحص اسمه ninhydrin test for free
+
+110
+00:08:29,380 --> 00:08:34,300
+alpha amino groupعلى Free Alpha Amino Group إذا
+
+111
+00:08:34,300 --> 00:08:38,020
+موجودة يتغير اللون وبيعطي visitant إذا مش موجودة
+
+112
+00:08:38,020 --> 00:08:41,740
+مش هيتغير اللون فال Free Alpha Amino Group هاي
+
+113
+00:08:41,740 --> 00:08:45,880
+بسميها ال reacting unit فحص التاني اسمه biuret
+
+114
+00:08:45,880 --> 00:08:50,100
+test بتدور على ال peptide bond لإنه فحص ال
+
+115
+00:08:50,100 --> 00:08:55,910
+xanthoprotect testZanto Proteic Test for Aromatic
+
+116
+00:08:55,910 --> 00:09:02,110
+Amino Acid لأ
+
+117
+00:09:02,110 --> 00:09:06,170
+الأحماض الأمنية اللي تحتوي على حلقة بنزين نشطة
+
+118
+00:09:06,170 --> 00:09:11,560
+نضيف هنا Activated Aromatic Amino Acidهنا فحص اسمه
+
+119
+00:09:11,560 --> 00:09:16,100
+hopkincol أو glycosilic acid reaction اللي هو اسمه
+
+120
+00:09:16,100 --> 00:09:20,280
+hopkincol، glycosilic acid reaction، بدور على
+
+121
+00:09:20,280 --> 00:09:24,780
+الاندولي جروب الـmelons test for amino acid
+
+122
+00:09:24,780 --> 00:09:29,260
+containing hydroxyl phenol group، للفينولي جروب،
+
+123
+00:09:29,260 --> 00:09:33,050
+ال amino acid اللي بيحتوي على فينولي جروبالسكيكوشي
+
+124
+00:09:33,050 --> 00:09:37,710
+تاست for ليجوانيدياميجرو لليجوانيدياميجرو
+
+125
+00:09:37,710 --> 00:09:42,570
+لتسالفايت تاست بيدور على ال self-hydraulic group
+
+126
+00:09:42,570 --> 00:09:45,970
+للحمض الأمين اللي يحتوي على ال self-hydraulic
+
+127
+00:09:45,970 --> 00:09:49,610
+group هو اللي هيعطيني positive غير هيك مش هيعطيني
+
+128
+00:09:49,610 --> 00:09:50,230
+positive
+
+129
+00:09:53,040 --> 00:09:56,300
+نجي نحكي عن أول فحص، أول فحص هو الـNilhedrine
+
+130
+00:09:56,300 --> 00:09:59,720
+الـNilhedrine، إيش الـobjective للفحص، إيش الهدف
+
+131
+00:09:59,720 --> 00:10:03,600
+للفحص؟ to detect the presence of free alpha amino
+
+132
+00:10:03,600 --> 00:10:07,820
+group، بيدور على الـfree alpha amino group، إذا
+
+133
+00:10:07,820 --> 00:10:11,880
+موجود free alpha amino group، هيتغير اللون،
+
+134
+00:10:11,880 --> 00:10:15,200
+بيعطيني لون بارد، في حالات اللي موجودة في free
+
+135
+00:10:15,200 --> 00:10:18,810
+alpha amino groupمين عنده فيه Alpha Amino Group؟
+
+136
+00:10:18,810 --> 00:10:21,810
+إذا ذاكرين الحمض الأميني، عشان الصيغة العامة له
+
+137
+00:10:21,810 --> 00:10:25,250
+تكون من الـ Center Carbon و من الـ Hydrogenة و من
+
+138
+00:10:25,250 --> 00:10:28,350
+الـ Side Chain و من Carboxyl Group و من Amino
+
+139
+00:10:28,350 --> 00:10:33,070
+Group Amino Group هاي سميناها Primary Amino Group
+
+140
+00:10:33,070 --> 00:10:37,330
+أو Alpha Amino Group معادة في حالة البرولين
+
+141
+00:10:37,330 --> 00:10:41,770
+سميناها Amino Groupيعني الأحماض الأمينية كلها
+
+142
+00:10:41,770 --> 00:10:47,190
+تحتوي على free alpha amino group ما عدا لبرولين
+
+143
+00:10:47,190 --> 00:10:51,030
+عنده amino group يعني مين هيعطيني positive مع هذا
+
+144
+00:10:51,030 --> 00:10:54,130
+الفحص لو أنا اشتغلت مجموعة أحماض أمينية على هذا
+
+145
+00:10:54,130 --> 00:10:57,570
+الفحص؟ كلهم هيعطوني positive لأنهم كلهم بيحتوي على
+
+146
+00:10:57,570 --> 00:10:59,130
+free alpha amino group
+
+147
+00:11:02,330 --> 00:11:04,690
+بالنسبة للـ objective to detect the presence of
+
+148
+00:11:04,690 --> 00:11:07,150
+free alpha amino group هل موجود free alpha amino
+
+149
+00:11:07,150 --> 00:11:10,710
+group أو لا إذا تغير اللغة يعني موجود إذا ما تغيرش
+
+150
+00:11:10,710 --> 00:11:13,950
+يعني مش موجود طيب إيش ال principle مبتدأ العمل
+
+151
+00:11:13,950 --> 00:11:17,730
+للفحص كيف يشتغل الفحص ال principle لو نعملها على
+
+152
+00:11:17,730 --> 00:11:21,330
+نظام معادلات بيسهل عليه كيف هذا الفحص له ال
+
+153
+00:11:21,330 --> 00:11:24,830
+principle أنه عندنا amino acid أنا بضيف حمض أميني
+
+154
+00:11:24,830 --> 00:11:26,850
+بضيف عليه نهيدرينرياشن
+
diff --git a/PL9fwy3NUQKwZ1Q7zOg1ObgufPMjRI3WY1/yXtOjcfTgyE.srt b/PL9fwy3NUQKwZ1Q7zOg1ObgufPMjRI3WY1/yXtOjcfTgyE.srt
new file mode 100644
index 0000000000000000000000000000000000000000..f66f04f9bb4525b0c4ff78f140d3ba082c816b3e
--- /dev/null
+++ b/PL9fwy3NUQKwZ1Q7zOg1ObgufPMjRI3WY1/yXtOjcfTgyE.srt
@@ -0,0 +1,1295 @@
+1
+00:00:00,930 --> 00:00:05,190
+اليوم ده نحكي بموضوع الإنزيمات إيش هنأخذ 
+
+2
+00:00:05,190 --> 00:00:09,450
+الإنزيمات، إيش العوامل اللي بتأثر على الإنزيم اللي
+
+3
+00:00:09,450 --> 00:00:14,990
+بتزيد من سرعة عمل الإنزيم والعوامل المثبطة للإنزيم
+
+4
+00:00:14,990 --> 00:00:19,990
+اللي بتقلل من عمل الإنزيم، اللي بتثبط الإنزيم
+
+5
+00:00:19,990 --> 00:00:24,270
+بيحكي ليه إن all chemical reactions in living
+
+6
+00:00:24,270 --> 00:00:27,930
+organisms require enzymes to work for building
+
+7
+00:00:27,930 --> 00:00:32,150
+breaking down molecules or speed up the reaction 
+
+8
+00:00:32,150 --> 00:00:37,250
+كل التفاعلات في جسم الإنسان تحتاج لوجود إنزيم سواء
+
+9
+00:00:37,250 --> 00:00:41,850
+بناء سواء هدم كل هذه for speed up the reaction
+
+10
+00:00:41,850 --> 00:00:47,690
+لتسريع التفاعل في عندنا حاجة اسمها enzyme kinetics
+
+11
+00:00:47,690 --> 00:00:54,660
+enzyme kinetics هي دراسة معدل حدوث التفاعل to study
+
+12
+00:00:54,660 --> 00:00:59,900
+the rate at which an enzyme works تفاعلات معدل
+
+13
+00:00:59,900 --> 00:01:06,560
+حدوث الإنزيم معدل عمل الإنزيم الإنزيم هو عبارة عن
+
+14
+00:01:06,560 --> 00:01:11,120
+biological catalyst محفز بيولوجي usually a
+
+15
+00:01:11,120 --> 00:01:16,840
+globular protein عادة هو عبارة عن بروتين produced
+
+16
+00:01:16,840 --> 00:01:21,180
+by living organisms يصنع في جسم الكائن الحي for
+
+17
+00:01:21,180 --> 00:01:24,640
+speed up specific chemical reactions without itself
+
+18
+00:01:24,640 --> 00:01:30,080
+being destroyed or changed in any way بدون ما يتغير بيسرع التفاعل
+
+19
+00:01:30,080 --> 00:01:35,100
+وظيفته إنه يسرع التفاعل الكيميائي بدون ما يستهلك
+
+20
+00:01:35,100 --> 00:01:40,320
+أو إنه يتدمر أو يصير عليه تغيير في هذا التفاعل إذن
+
+21
+00:01:40,320 --> 00:01:45,440
+هو محفز بيولوجي عادة بيكون عبارة عن بروتين بينتج
+
+22
+00:01:45,440 --> 00:01:49,860
+في أجسام الكائنات الحية لتسريع التفاعل بدون ما
+
+23
+00:01:49,860 --> 00:01:54,380
+يصير عليه استهلاك أو تدمير أو تغيير، إنما بيخرج من
+
+24
+00:01:54,380 --> 00:01:58,520
+التفاعل زي ما دخل. They are produced by the
+
+25
+00:01:58,520 --> 00:02:01,280
+living organisms and are usually present in only
+
+26
+00:02:01,280 --> 00:02:05,040
+very small amounts in various cells، بينتج في أجسام
+
+27
+00:02:05,040 --> 00:02:08,780
+الكائنات الحية وبالعادة بيكون بكميات قليلة.
+
+28
+00:02:11,180 --> 00:02:19,500
+ما هي خصائص الإنزيم؟ خصائص الإنزيم الإنزيم
+
+29
+00:02:19,500 --> 00:02:23,960
+لا يستهلك في التفاعل، بيدخل على التفاعل بيعمل على
+
+30
+00:02:23,960 --> 00:02:28,080
+تسريع التفاعل الكيميائي بدون ما يستهلك في التفاعل،
+
+31
+00:02:28,080 --> 00:02:33,040
+بعد ما يخلص التفاعل، بيطلع منه زي ما دخل إنزيم لا
+
+32
+00:02:33,040 --> 00:02:38,120
+يتغير من نفسه، ولا بيسير عليه حتى أي تغيير، زي ما
+
+33
+00:02:38,120 --> 00:02:42,740
+دخل بيطلع reused again for the same reaction with
+
+34
+00:02:42,740 --> 00:02:46,640
+other molecules أقدر أستخدمه مرة تانية لنفس
+
+35
+00:02:46,640 --> 00:02:51,740
+التفاعل مع مادة تانية بستخدمه مرة تانية لنفس
+
+36
+00:02:51,740 --> 00:02:56,160
+التفاعل مع مادة تانية most enzymes are proteins
+
+37
+00:02:56,160 --> 00:02:59,800
+حكينا أنه usually عادة الإنزيم بيكون عبارة عن
+
+38
+00:02:59,800 --> 00:03:04,600
+بروتين و globular protein يعني بيكون tertiary أو 
+
+39
+00:03:04,600 --> 00:03:08,560
+quaternary act as a catalyst to accelerate the
+
+40
+00:03:08,560 --> 00:03:13,600
+reaction إنه بيعمل كمحفز لتسريع التفاعل are
+
+41
+00:03:13,600 --> 00:03:18,060
+specific for what they will catalyze وهو متخصص
+
+42
+00:03:18,060 --> 00:03:26,540
+لتفاعل معين كل إنزيم متخصص لأنه يسرع تفاعل معين كيف
+
+43
+00:03:26,540 --> 00:03:30,980
+بيشتغل الـ enzyme؟ الـ enzyme بيشتغل إنه عندنا الـ
+
+44
+00:03:30,980 --> 00:03:34,640
+enzyme فيه موقع اسمه الـ active site الـ active
+
+45
+00:03:34,640 --> 00:03:38,600
+site هو موقع نشط بترتبط فيه الـ substrate اللي بدنا
+
+46
+00:03:38,600 --> 00:03:43,100
+نسرع إليها التفاعل في موقع في الـ enzyme اسمه الـ
+
+47
+00:03:43,100 --> 00:03:47,300
+active site هذا بيشبه الشيء اللي أنا بدي أسرعه،
+
+48
+00:03:47,300 --> 00:03:51,080
+بيشبه الـ substrate يعني كل enzyme متخصص حاجة 
+
+49
+00:03:51,080 --> 00:03:55,670
+لتفاعل معين كيف متخصص إنه فيه موقع «active site»
+
+50
+00:03:55,670 --> 00:03:59,050
+بيشبه المادة اللي بدي أسرعها؟ يعني على فرض في هذه
+
+51
+00:03:59,050 --> 00:04:02,190
+المعادلة، في هذه الدورة، إيش عندي؟ على الجنب،
+
+52
+00:04:02,190 --> 00:04:06,830
+بالأخصر، على الشمال، عندي substrate اسمه السكروز،
+
+53
+00:04:06,830 --> 00:04:10,450
+سكر السكروز، هذا سكر ثنائي عبارة عن اتحاد
+
+54
+00:04:10,450 --> 00:04:14,470
+جلوكوز وفركتوز، نعمله تكسير، نعمله عملية
+
+55
+00:04:14,470 --> 00:04:19,210
+breakdown عشان نتكون جلوكوز وفركتوز فبيجي هذا
+
+56
+00:04:19,210 --> 00:04:21,470
+الـ substrate اللي هو الـ Saccharose بيروح على
+
+57
+00:04:21,470 --> 00:04:25,970
+الإنزيم المتخصص له بيرتبط في الموقع اللي اسمه
+
+58
+00:04:25,970 --> 00:04:30,230
+active site في الفراغ اللي بالوسط، الإنزيم،
+
+59
+00:04:30,230 --> 00:04:34,150
+الإنزيم هو هذا اللي موجود فيه في الوسط فراغ، بيشبه
+
+60
+00:04:34,150 --> 00:04:36,790
+هذا الفراغ بيشبه مين؟ بيشبه الـ Saccharose، الـ
+
+61
+00:04:36,790 --> 00:04:39,950
+substrate بتيجي هذه الـ Saccharose بترتبط في هذا
+
+62
+00:04:39,950 --> 00:04:42,990
+الموقع، في الـ active site بيكون الإنزيم مع
+
+63
+00:04:42,990 --> 00:04:46,900
+substrate complex بيصير التفاعل اللي هو عبارة عن
+
+64
+00:04:46,900 --> 00:04:50,100
+تكسير الـ substrates فبتتحول الـ substrates إلى
+
+65
+00:04:50,100 --> 00:04:54,200
+product دخلت و بدأت تتحول الحين ل product، لنواتج
+
+66
+00:04:54,200 --> 00:04:57,840
+نواتج هي مرتبطة في الإنزيم، فبنسميه إنزيم مع
+
+67
+00:04:57,840 --> 00:05:02,060
+product بعد هي بتتحرر الـ product هذه النواتج بتطلع
+
+68
+00:05:02,060 --> 00:05:06,340
+برا الإنزيم والإنزيم بيطلع زي ما دخل بدون أي تغيير
+
+69
+00:05:06,340 --> 00:05:11,540
+الـ product بتتحرر من الإنزيم والإنزيم بيطلع زي ما
+
+70
+00:05:11,540 --> 00:05:16,650
+هو بدون أي تغيير عندنا المعادلة اللي كيف بيشتغل
+
+71
+00:05:16,650 --> 00:05:20,230
+الإنزيم أول حاجة بتيجي إنزيم ترتبط مع substrate
+
+72
+00:05:20,230 --> 00:05:23,730
+إنزيم زائد substrate بيعطيني إنزيم و substrate
+
+73
+00:05:23,730 --> 00:05:27,190
+complex بعدين substrate بتتحول ل product فبيعطيني
+
+74
+00:05:27,190 --> 00:05:31,810
+إنزيم زائد product الـ substrate اتحولت إلى نواتج
+
+75
+00:05:31,810 --> 00:05:37,240
+إلى product كيف بيشتغل الإنزيم مع الـ Substrate؟
+
+76
+00:05:37,240 --> 00:05:41,020
+كيف بيرتبطه؟ حكينا بموقع اسمه الـ Active site هذا
+
+77
+00:05:41,020 --> 00:05:46,200
+الموقع بس له تركيب مشابه لتركيب الـ Substrate اللي
+
+78
+00:05:46,200 --> 00:05:52,600
+خاص في هذا الإنزيم على مبدأ اسمه lock and key
+
+79
+00:05:52,600 --> 00:05:57,200
+hypothesis اللي هو خاصية القفل والمفتاح زي مثلا
+
+80
+00:05:57,200 --> 00:06:01,160
+عندنا لما يكون عندنا قفل هاد بس وإله مفتاح
+
+81
+00:06:01,160 --> 00:06:05,700
+واحد بيفتحه بيدخل فيه وبيقدر يفتحه والإنزيم زي هاد
+
+82
+00:06:05,700 --> 00:06:09,900
+عنده موقع اسمه active site بترتبط معاه substrate
+
+83
+00:06:09,900 --> 00:06:13,820
+متخصصة فيه بس هو إله بيشبهه هذا الموقع اللي بيشبه
+
+84
+00:06:13,820 --> 00:06:17,260
+زي إن هنا القطعة اللي لونها زهر هي اللي على
+
+85
+00:06:17,260 --> 00:06:21,690
+الجنب هذا الـ substrate والأصفر الإنزيم طالع على
+
+86
+00:06:21,690 --> 00:06:24,910
+الإنزيم من الجنب فيه موقع اسمه الـ Active site
+
+87
+00:06:24,910 --> 00:06:29,210
+بيشبه تركيب الـ substrate بتيجي الـ substrates بترتبط
+
+88
+00:06:29,210 --> 00:06:33,750
+في هذا الموقع بيكون إنزيم substrate complex بتكون
+
+89
+00:06:33,750 --> 00:06:37,330
+هذا الـ complex بيصير التفاعل إن الـ substrates هيدا
+
+90
+00:06:37,330 --> 00:06:41,970
+بتتحول لـ product بتتحول لـ product مرتبطة في الإنزيم
+
+91
+00:06:41,970 --> 00:06:46,090
+فبتكون الإنزيم و product بعد هيك بيتحرر الـ product
+
+92
+00:06:46,090 --> 00:06:50,970
+بتطلع و بيطلع الإنزيم زي ما هو زي ما دخل و بيطلع و
+
+93
+00:06:50,970 --> 00:06:57,850
+بقدر استخدمه مرة تانية في تفاعل ثاني أجي أنا عندي
+
+94
+00:06:57,850 --> 00:07:02,330
+تفاعل أي تفاعل كيف بدي أخليه يكون سريع making
+
+95
+00:07:02,330 --> 00:07:06,310
+reactions go faster يعني بدي أسرع التفاعل بيحكيلي
+
+96
+00:07:06,310 --> 00:07:10,230
+عند رفع درجة الحرارة الجزيئات بتتحرك أسرع
+
+97
+00:07:10,230 --> 00:07:12,950
+increasing the temperature makes molecules move
+
+98
+00:07:12,950 --> 00:07:17,410
+faster إذا أنا رفعت درجة الحرارة الجزيئات بتتحرك
+
+99
+00:07:17,410 --> 00:07:24,120
+أسرع فالتفاعل هيكون أسرع لكن biological systems are
+
+100
+00:07:24,120 --> 00:07:27,660
+very sensitive to temperature changes إن جسم
+
+101
+00:07:27,660 --> 00:07:32,180
+الإنسان حساس للتغير في درجة الحرارة إذا كانت في
+
+102
+00:07:32,180 --> 00:07:35,280
+عندي تفاعل في جسم الإنسان، في داخل جسم الإنسان
+
+103
+00:07:35,280 --> 00:07:39,140
+وهذا التفاعل أنا بدي أسرعه لو رفعت درجة الحرارة
+
+104
+00:07:39,140 --> 00:07:43,340
+هيأثر على جسم الإنسان فإن رفع درجات الحرارة
+
+105
+00:07:43,340 --> 00:07:48,060
+لتفاعلات بتحدث داخل جسم الإنسان بتأثر و تأثير عنها
+
+106
+00:07:48,060 --> 00:07:52,340
+تأثيرات، بينتج عنها تأثيرات فكيف بدي أسرع التفاعل
+
+107
+00:07:52,340 --> 00:07:56,380
+في داخل جسم الإنسان؟ بالإنزيم enzymes can increase
+
+108
+00:07:56,380 --> 00:07:58,760
+the rate of reactions without increasing the
+
+109
+00:07:58,760 --> 00:08:03,500
+temperature استخدم إنزيم بيرفع معدل حدوث التفاعل
+
+110
+00:08:03,500 --> 00:08:09,040
+بدون ما يزود درجة الحرارة without increasing the
+
+111
+00:08:09,040 --> 00:08:13,400
+temperature الإنزيم بيسرع التفاعل بدون زيادة درجة
+
+112
+00:08:13,400 --> 00:08:16,740
+الحرارة طب كيف بيشتغل الإنزيم؟ بيزودش درجة الحرارة،
+
+113
+00:08:16,740 --> 00:08:21,280
+شو بيسوي؟ بيقلل حاجة بنسميها ال activation energy،
+
+114
+00:08:21,280 --> 00:08:26,480
+اللي هي طاقة التنشيط، بيقلل الطاقة اللازمة لحدوث
+
+115
+00:08:26,480 --> 00:08:32,100
+التفاعل إذن الإنزيم بيقلل حدوث التفاعل عن طريق
+
+116
+00:08:32,100 --> 00:08:36,900
+تقليل الطاقة اللازمة وبالتالي بيسرع التفاعل بدون
+
+117
+00:08:36,900 --> 00:08:41,960
+تغيير درجة الحرارة لأنه إذا غيرت درجة الحرارة داخل
+
+118
+00:08:41,960 --> 00:08:45,880
+جسم الإنسان لها تأثيرات ممكن يصير denaturation
+
+119
+00:08:45,880 --> 00:08:50,380
+تدمير للإنزيمات لأن معظم الإنزيمات هي بروتينات
+
+120
+00:08:50,380 --> 00:08:58,010
+فهتتأثر بدرجة الحرارة هنا عندنا رسم، رسم ليه؟ إنه
+
+121
+00:08:58,010 --> 00:09:02,870
+التفاعل كده هيستهلك طاقة لو كان موجود الإنزيم، ولو
+
+122
+00:09:02,870 --> 00:09:05,870
+التفاعل ماشي بدون وجود الإنزيم، كده هياخد طاقة
+
+123
+00:09:05,870 --> 00:09:10,890
+الخط المتقطع، اللي هو قمة أعلى، عند القمة، الخط
+
+124
+00:09:10,890 --> 00:09:15,490
+المتقطع مش مكتوب activation energy without  انزعج،
+
+125
+00:09:15,490 --> 00:09:21,360
+إنه هانحدوث التفاعل بدون وجود الإنزيم استهلك طاقة
+
+126
+00:09:21,360 --> 00:09:25,200
+أعلى إذا الطاقة اللي استهلاكها التفاعل في عدم وجود
+
+127
+00:09:25,200 --> 00:09:29,480
+الإنزيم هتكون أعلى يعني هيمشي التفاعل أبطأ في الخط
+
+128
+00:09:29,480 --> 00:09:33,440
+اللي تحته مكتوب activation energy with enzyme 
+
+129
+00:09:33,440 --> 00:09:38,540
+قديش استهلك طاقة في وجود الإنزيم كانت الطاقة أقل
+
+130
+00:09:38,540 --> 00:09:44,440
+فبنستنتج أن الإنزيم يعمل على تقليل الطاقة اللازمة
+
+131
+00:09:44,440 --> 00:09:50,290
+للتفاعل و يسرع التفاعل بدون الحاجة إلى تغيير درجة
+
+132
+00:09:50,290 --> 00:09:55,960
+الحرارة عوامل بتأثر على نشاط الإنزيم factors
+
+133
+00:09:55,960 --> 00:10:00,380
+affecting enzyme activity ما هي العوامل اللي بتأثر
+
+134
+00:10:00,380 --> 00:10:04,040
+على نشاط الإنزيم؟ في عندنا عدة عوامل أول عامل
+
+135
+00:10:04,040 --> 00:10:07,820
+substrate concentration تركيز الـ substrate اللي هي
+
+136
+00:10:07,820 --> 00:10:11,220
+المادة اللي بدأ ترتبط مع الإنزيم عشان ينشطها عشان
+
+137
+00:10:11,220 --> 00:10:15,020
+يسرع التفاعل فالتركيز المادة بيأثر على نشاط
+
+138
+00:10:15,020 --> 00:10:20,410
+الإنزيم الـ pH درجة الـ pH بتأثر على نشاط الإنزيم
+
+139
+00:10:20,410 --> 00:10:25,170
+temperature درجة الحرارة بتأثر الـ inhibitors اللي
+
+140
+00:10:25,170 --> 00:10:31,730
+هي المثبطات بتثبط عمل الإنزيم هتأثر أكيد على نشاط
+
+141
+00:10:31,730 --> 00:10:37,130
+الإنزيم هندرس واحدة واحدة من هدول العوامل نيجي
+
+142
+00:10:37,130 --> 00:10:39,650
+لأول عامل من العوامل اللي هو الـ substrate
+
+143
+00:10:39,650 --> 00:10:44,490
+concentration تركيز الـ substrate كيف يؤثر على نشاط
+
+144
+00:10:44,490 --> 00:10:49,200
+الإنزيم اللي جيت لو نعتبر أنه عندي إنزيمات موجودة
+
+145
+00:10:49,200 --> 00:10:53,040
+هذه الإنزيمات كل إنزيم له substrate ييجي الإنزيم
+
+146
+00:10:53,040 --> 00:10:56,820
+هذه بيرتبط مع الـ substrate في موقع الـ active site
+
+147
+00:10:56,820 --> 00:11:01,300
+بيرتبط الإنزيم بالـ substrate لما ما هيسرع التفاعل
+
+148
+00:11:01,300 --> 00:11:05,060
+إذا أجي إنزيم ثاني ويرتبط بالـ substrate هيزيد
+
+149
+00:11:05,060 --> 00:11:09,040
+التفاعل أسرع من الأول من واحد لحاله لما يكونوا
+
+150
+00:11:09,040 --> 00:11:12,340
+اثنين هل هيكون التفاعل أسرع لما يكون عندي إنزيم
+
+151
+00:11:12,340 --> 00:11:16,040
+واحد مرتبط بـ substrate أو إنزيمين وكل واحد مرتبط
+
+152
+00:11:16,040 --> 00:11:19,980
+بـ substrate أكيد وجود إنزيمين هيسرع التفاعل أكثر
+
+153
+00:11:19,980 --> 00:11:23,580
+ولو جبت كمان إنزيم ثالث مرتبط بـ substrate هيسرع
+
+154
+00:11:23,580 --> 00:11:28,040
+التفاعل أكثر والرابع برضه هيسرع التفاعل أكثر وهكذا
+
+155
+00:11:28,820 --> 00:11:33,180
+فكل ما زاد الـ substrate concentration تركيز الـ
+
+156
+00:11:33,180 --> 00:11:37,300
+substrate كل ما زاد تركيز الـ substrate هيزيد
+
+157
+00:11:37,300 --> 00:11:42,240
+الارتباط بين إنزيم و substrate هيسرع التفاعل أكثر
+
+158
+00:11:42,240 --> 00:11:48,260
+يعني العلاقة طردية ما بين تركيز الـ substrate و
+
+159
+00:11:48,260 --> 00:11:54,420
+سرعة التفاعل كل ما أضيف substrate بتزيد سرعة
+
+160
+00:11:54,420 --> 00:11:59,600
+التفاعل فالعلاقة طردية the increase in velocity is
+
+161
+00:11:59,600 --> 00:12:03,200
+proportional to the substrate concentration إن
+
+162
+00:12:03,200 --> 00:12:08,020
+سرعة التفاعل زيادة سرعة التفاعل هي علاقة طردية مع
+
+163
+00:12:08,020 --> 00:12:14,130
+تركيز الـ substrate هنا المخطط الثاني اللي حكيناه
+
+164
+00:12:14,130 --> 00:12:18,130
+بالأول إن العلاقة طردية بين تركيز الـ substrate
+
+165
+00:12:18,130 --> 00:12:24,340
+وسرعة التفاعل الكيميائي طيب، هل هذه العلاقة طردية إلى
+
+166
+00:12:24,340 --> 00:12:28,580
+ما لا نهايتها تستمر طردية إن أنا كل ما أضيف substrate
+
+167
+00:12:28,580 --> 00:12:32,660
+هتزيد سرعة التفاعل كل ما أضيف substrate بتزيد سرعة
+
+168
+00:12:32,660 --> 00:12:38,040
+التفاعل؟ لا، هذه العلاقة إلها حد معين كافي يعني،
+
+169
+00:12:38,040 --> 00:12:42,340
+أنا جيت لنفرض إن هذا المكان فيه أربع إنزيمات بس،
+
+170
+00:12:42,340 --> 00:12:46,520
+لو أنا أضفت substrate، بيرتبط مع الإنزيم الأول و
+
+171
+00:12:46,520 --> 00:12:51,020
+بيسرع التفاعل لو ضفتني substrate ثاني بيرتبط مع
+
+172
+00:12:51,020 --> 00:12:55,140
+الإنزيم الثاني والتفاعل بيمشي أسرع أكثر طب لو ضفت
+
+173
+00:12:55,140 --> 00:12:58,360
+substrate ثالث برضه هيرتبط مع الإنزيم الثالث
+
+174
+00:12:58,360 --> 00:13:03,060
+الموجود ويسرع التفاعل أكثر يعني كل ما أضيف
+
+175
+00:13:03,060 --> 00:13:05,900
+substrate بيرتبط مع الإنزيم بيسرع التفاعل بيصير
+
+176
+00:13:05,900 --> 00:13:09,960
+أسرع حتى الآن العلاقة طردية طب عندي
+
+201
+00:14:54,830 --> 00:14:59,710
+إنزيم substrate مهما أضيف substrate مش هيأثر على
+
+202
+00:14:59,710 --> 00:15:04,030
+سرعته التفاعل الكيميائي فكون أنا وصلت لحاجة اسمها
+
+203
+00:15:04,030 --> 00:15:09,590
+الـ Vmax، الـ maximum velocity هي أقصى سرعة يصل
+
+204
+00:15:09,590 --> 00:15:14,350
+إليها التفاعل الكيميائي، وعندها بتكون كل الإنزيمات 
+
+205
+00:15:14,350 --> 00:15:19,470
+متشبعة مرتبطة بـ substrate، هل جيت بيحكيلي عن نص
+
+206
+00:15:19,470 --> 00:15:24,710
+Vmax، نص Vmax، نص السرعة القصوى هاي يعني نصها في
+
+207
+00:15:24,710 --> 00:15:31,830
+حاجة اسمها KM، يعني نص Vmax في حاجة اسمها KM، إيش هي 
+
+208
+00:15:31,830 --> 00:15:38,510
+KM؟ KM هي تركيز الـ substrate اللازم للوصول إلى نص
+
+209
+00:15:38,510 --> 00:15:44,530
+سرعة التفاعل الكيميائي، KM هي تركيز الـ substrate
+
+210
+00:15:44,530 --> 00:15:50,230
+اللازم للوصول إلى نص سرعة التفاعل الـ factor
+
+211
+00:15:50,230 --> 00:15:54,730
+reaction faster reaction, but it reach a saturation
+
+212
+00:15:54,730 --> 00:15:58,730
+point when all the enzyme molecules are occupied
+
+213
+00:15:58,730 --> 00:16:03,280
+للـ Vmax، بحكي لأن العلاقة طردية صحيح بين 
+
+214
+00:16:03,280 --> 00:16:06,620
+الـSubstration، بين الـSubstrate، Concentration،
+
+215
+00:16:06,620 --> 00:16:10,700
+بين تركيز الـSubstrate وسرعة التفاعل، إنه هيسرع
+
+216
+00:16:10,700 --> 00:16:15,700
+التفاعل، ولكن لعند متى؟ الوصول إلى نقطة
+
+217
+00:16:15,700 --> 00:16:20,280
+الـSaturation Point، نقطة التشبع، عندها بتكون كل 
+
+218
+00:16:20,280 --> 00:16:25,600
+الإنزيمات مرتبطة بـSubstrate، فبتكون، بنسميها،
+
+219
+00:16:25,600 --> 00:16:31,270
+«High V Max»، في عندنا ميشيل و مينتن اللي هم عالمان
+
+220
+00:16:31,270 --> 00:16:34,470
+حطوا قانون بيحكي أنه at low substrate
+
+221
+00:16:34,470 --> 00:16:37,970
+concentration the enzyme is not saturated with the
+
+222
+00:16:37,970 --> 00:16:41,430
+substrate and the reaction is not proceeding at
+
+223
+00:16:41,430 --> 00:16:45,010
+maximum velocity whereas when the enzyme is
+
+224
+00:16:45,010 --> 00:16:48,410
+saturated with substrate maximum velocity is
+
+225
+00:16:48,410 --> 00:16:53,160
+observed، عايش ملخص هذا الحكي أنه لما يكون تركيز ال
+
+226
+00:16:53,160 --> 00:16:57,260
+substrate قليل، الإنزيمات بتكونش متشبعة بال
+
+227
+00:16:57,260 --> 00:17:00,860
+substrate، بتكونش كل الإنزيمات مرتبطة بال substrate
+
+228
+00:17:00,860 --> 00:17:05,420
+مهما كل substrate بيرتبط مع أنزيم ولكن غير متشبع
+
+229
+00:17:05,420 --> 00:17:09,310
+لأن تركيز ال substrate لسه قليل، في هذه الحالة مش
+
+230
+00:17:09,310 --> 00:17:13,090
+هنوصل للـ maximum velocity، إلى أقصى سرعة للتفاعل،
+
+231
+00:17:13,090 --> 00:17:17,930
+مش هنوصلها، بينما لما تكون الانزيمات متشبعة بال
+
+232
+00:17:17,930 --> 00:17:21,190
+substrate، خلاص كل الانزيمات مرتبطة بال substrate،
+
+233
+00:17:21,190 --> 00:17:25,510
+فأنا باصل لـ maximum velocity اللي عندها بتكون 
+
+234
+00:17:25,510 --> 00:17:31,110
+نقطة التشبع، ليه الـ saturated point؟ عشان هيك العلاقة
+
+235
+00:17:31,110 --> 00:17:35,370
+طردية ما بين تركيز الـsubstrate وسرعة التفاعل 
+
+236
+00:17:35,370 --> 00:17:39,210
+الكيميائي، لكنها مش علاقة طردية لما لا نهاية ولا
+
+237
+00:17:39,210 --> 00:17:43,910
+إنما وصولها إلى نقطة الـsaturated point، إلى نقطة
+
+238
+00:17:43,910 --> 00:17:49,390
+التشبع، بيثبت المنحنى، وتبطل العلاقة طردية، بيصيرش كل 
+
+239
+00:17:49,390 --> 00:17:55,030
+ما يزيد تركيز الـsubstrate أنه يزيد سرعة التفاعل
+
+240
+00:17:56,370 --> 00:18:00,070
+هنا عندنا القانون «ميشيلز أنمينتين إكوايشن»، هذا
+
+241
+00:18:00,070 --> 00:18:03,590
+القانون مش مطلوب منها، بس ضروري أننا نعرفه، الـKM
+
+242
+00:18:03,590 --> 00:18:08,630
+اللي هي تركيز السبسترات عند نص Vmax، الـKM حكينا 
+
+243
+00:18:08,630 --> 00:18:13,990
+عنها قبل هيك اللي هي تركيز السبسترات عند نص Vmax،
+
+244
+00:18:13,990 --> 00:18:17,870
+عامل التاني من العوامل اللي بتأثر على نشاط الإنزيم 
+
+245
+00:18:17,870 --> 00:18:21,770
+هو الـpH، الـpH بيحكي الـextreme pH level
+
+246
+00:18:21,770 --> 00:18:26,180
+والـdenaturated enzyme، الـ pH، التغيير في الـ pH
+
+247
+00:18:26,180 --> 00:18:31,080
+بيعمل denaturation للإنزيم، بيعمل تشوّه لشكل
+
+248
+00:18:31,080 --> 00:18:35,080
+الإنزيم، بيعمل denaturation يعني تشوّه لشكل الإنزيم
+
+249
+00:18:35,080 --> 00:18:40,060
+معظم إنزيمات اللي في جسم الإنسان بتشتغل عند pH من
+
+250
+00:18:40,060 --> 00:18:45,160
+6 إلى 8، most human enzyme work at pH من 6 إلى 80
+
+251
+00:18:45,160 --> 00:18:49,240
+بناء على عش أنه هي من 6 إلى 8، depend on its
+
+252
+00:18:49,240 --> 00:18:52,780
+location in the body، في إنزيمات برضه بتختلف،
+
+253
+00:18:52,780 --> 00:18:57,320
+بتشتغلش من 6 ل 8، بناء على موقعها في جسم الإنسان،
+
+254
+00:18:57,320 --> 00:19:00,940
+وين هي موجودة، في أي مكان زي مثلا الـPepsin، هذا
+
+255
+00:19:00,940 --> 00:19:05,120
+موجود في الـstomach، في المعدة، ولما المعدة وسطها
+
+256
+00:19:05,120 --> 00:19:08,860
+حمضي، فهو بيشتغل على pH زي المكان اللي هو فيه،
+
+257
+00:19:08,860 --> 00:19:13,120
+بيعمل على pH 3، الـ Trypsin موجود في الـ small
+
+258
+00:19:13,120 --> 00:19:17,140
+intestine، في الأمعاء، والأمعاء وسطها قاعدة فهذا
+
+259
+00:19:17,140 --> 00:19:21,040
+بيشتغل عند pH 8 زي المكان اللي هو موجود فيه
+
+260
+00:19:21,040 --> 00:19:24,880
+فالإنزيمات الموجودة في الـ GSM تشتغل عند pH حسب
+
+261
+00:19:24,880 --> 00:19:29,850
+المكان اللي هي موجودة فيه، معظمها عند 6 لـ 8، طب لو
+
+262
+00:19:29,850 --> 00:19:33,130
+اتغيرت ال pH لهذا الانزيم يعني مثلا ال بيبسين 
+
+263
+00:19:33,130 --> 00:19:36,630
+بيشتغل عند تلاتة لو حطته على تمانية، يشتغل عند pH
+
+264
+00:19:36,630 --> 00:19:40,370
+تمانية بيحكي اللي بيصير تشوه في شكل الانزيم، بيصير
+
+265
+00:19:40,370 --> 00:19:43,690
+عليه denaturation، فال structure of انزيم is
+
+266
+00:19:43,690 --> 00:19:48,190
+changed، شكل الانزيم وتركيبته هتختلف لما يصير تشوه
+
+267
+00:19:48,190 --> 00:19:53,150
+للانزيم، تركيب هذا الانزيم ممكن يختلف، هيختلف، إيش
+
+268
+00:19:53,150 --> 00:19:55,910
+ممكن يختلف فيه؟ الـ Active site، موقع الـ Active
+
+269
+00:19:55,910 --> 00:19:59,930
+site، حكينا هذا ملائم للـ Substrate، شكله زي شكل الـ
+
+270
+00:19:59,930 --> 00:20:02,190
+Substrate، بتيجي ال Substrate بترتبط في هذا الـ
+
+271
+00:20:02,190 --> 00:20:06,890
+Active site، إذا تغيرت الـ pH، بيصير فينا تشوه فشكل
+
+272
+00:20:06,890 --> 00:20:11,490
+الـ Active site، هذا مع التشوه هيتغير هيبطل ملائم
+
+273
+00:20:11,490 --> 00:20:14,410
+لل Substrate، بتيجي ال Substrate ده ترتبط مع
+
+274
+00:20:14,410 --> 00:20:18,250
+الانزيم في موقع الـ Active site، بتلاقي بتقدرش
+
+275
+00:20:18,250 --> 00:20:21,850
+ترتبط فيه لأنه مش ملائم لشكلها، بتقدرش تيجي ترتبط
+
+276
+00:20:21,850 --> 00:20:25,510
+فيه، الـ active site is distorted and the substrate
+
+277
+00:20:25,510 --> 00:20:29,510
+molecule will no longer fit in it، أنه ال active
+
+278
+00:20:29,510 --> 00:20:33,510
+site صار عليه تشوي وصار تدمين فال substrate
+
+279
+00:20:33,510 --> 00:20:37,810
+molecule مش ملائم لها هذا الشكل، مش هتقدر تيجي
+
+280
+00:20:37,810 --> 00:20:39,510
+ترتبط مع الانزيم
+
+281
+00:20:43,260 --> 00:20:49,000
+هنا مخطط بيوضّح أن الـ pH، optimum pH للبيبسين،
+
+282
+00:20:49,000 --> 00:20:52,420
+هيله بالأحمر، البيبسين القمة اللي هو هين بيشتغل عند
+
+283
+00:20:52,420 --> 00:20:56,360
+تلاتة، أعلى اللي عمل لهذا الانزيم عند pH تلاتة
+
+284
+00:20:56,360 --> 00:21:00,160
+لتريبسين أعلى pH اللي هو الأزراج، القمة اللي هو
+
+285
+00:21:00,160 --> 00:21:03,380
+أعلى يشير عند تمانية، أعلى reaction اللي هو بيكون
+
+286
+00:21:03,380 --> 00:21:09,640
+عند pH تمانية، العامل اللي بعدين بيأثر على رشاط 
+
+287
+00:21:09,640 --> 00:21:13,850
+الانزيم هو الـ temperature، درجة الحرارة، بحكي لدرجة 
+
+288
+00:21:13,850 --> 00:21:18,030
+الحرارة اللي بتأثر على معدل الإنزيم، optimum
+
+289
+00:21:18,030 --> 00:21:21,890
+temperature، أفضل درجة حرارة لعمل الإنزيم ويكون
+
+290
+00:21:21,890 --> 00:21:25,490
+نشيط، اللي هو نشاط الإنزيم في عالة نشاطهم الـ human
+
+291
+00:21:25,490 --> 00:21:29,290
+enzyme work at 35 درجة إلى 40 درجة سليزيا اللي هي
+
+292
+00:21:29,290 --> 00:21:34,310
+ال body temperature 37 درجة سليزيا ببعين ال range
+
+293
+00:21:34,310 --> 00:21:38,190
+درجة حرارة جسم الإنسان من 35 إلى 40 درجة سليزيا
+
+294
+00:21:39,320 --> 00:21:43,900
+بيعمل الانزين بيكون optimum temperature إليه بيكون
+
+295
+00:21:43,900 --> 00:21:48,420
+أعلى نشاط للانزين، طب لو ارتفعت درجة الحرارة، rise ال
+
+296
+00:21:48,420 --> 00:21:52,500
+temperature أعلى من 40 درجة سليزيا، بيصير عندنا 
+
+297
+00:21:52,500 --> 00:21:56,800
+denaturation للانزين، لأنه أحكي إن بعض الانزين ماتي
+
+298
+00:21:56,800 --> 00:21:59,140
+عبارة عن البروتين، بيصير الـdenaturation،
+
+299
+00:21:59,140 --> 00:22:03,110
+الـdenaturation يعني اتشوه، يعني تركيب اختلاف، unfold
+
+300
+00:22:03,110 --> 00:22:07,930
+تركيب تختلفات، lose shape هيفقد شكله، إذا شكل الـ
+
+301
+00:22:07,930 --> 00:22:12,090
+active site مش هيكون ملائم لل substrate، طيب لو
+
+302
+00:22:12,090 --> 00:22:15,950
+lower temperature، لو قلة درجة الحرارة أقل من 35
+
+303
+00:22:15,950 --> 00:22:22,030
+المركبات هتكون بتتحرك ببطء، molecules move slower
+
+304
+00:22:22,030 --> 00:22:28,010
+هتتحرك المركبات ببطء بتكون تمشي بشكل باطين، وبالتالي
+
+305
+00:22:28,010 --> 00:22:32,870
+التصادمات ما بين الـ substrate والإنزيم هتجل، هيكون
+
+306
+00:22:32,870 --> 00:22:37,410
+لأن المراكبات بتتحرك ببطء، فهي بتتحرك و تروح على
+
+307
+00:22:37,410 --> 00:22:42,110
+الإنزيم، فلما تروح تتحرك ببطء هتقل التصادمات بينها
+
+308
+00:22:42,110 --> 00:22:45,970
+وبين الإنزيم، هيقل التقاء ال substrate مع الإنزيم
+
+309
+00:22:45,970 --> 00:22:50,030
+فمش هيرتبطوا مع بعض، فبتجل سرعات التفاعل الكيميائي
+
+310
+00:22:50,030 --> 00:22:54,390
+ويجل نشاط الإنزيم، إذا درجة الحرارة بتأثر، إذا 
+
+311
+00:22:54,390 --> 00:22:58,990
+ارتفعت بيصيب دي نات يوراجين، إذا قلت، بتقل التصادمات
+
+312
+00:22:58,990 --> 00:23:02,050
+ما بين الـ substrate والإنزيم، optimum temperature
+
+313
+00:23:02,050 --> 00:23:05,870
+بيعمل عليها الجسم، عليها الهيمينزيمات هي زي درجة
+
+314
+00:23:05,870 --> 00:23:12,710
+حرارة الجسم، ما هي ما بين 35 و40 درجة سيليزيا، هنا
+
+315
+00:23:12,710 --> 00:23:16,070
+عندنا مخطط رسم لـ temperature ونشاط الانزيم
+
+316
+00:23:16,070 --> 00:23:19,350
+temperature and enzyme activity in human، زي ما 
+
+317
+00:23:19,350 --> 00:23:22,770
+حكينا أنه أعلى نشاط للانزيم، optimum temperature
+
+318
+00:23:22,770 --> 00:23:26,470
+for enzyme activity، عند القمة أعلى إشي بيكون عند
+
+319
+00:23:26,470 --> 00:23:31,850
+37 درجة سليزيا، عند هذه الدرجة بتكون الأعلى نشاط
+
+320
+00:23:31,850 --> 00:23:36,570
+للانزيم، نفس درجة حرارة جسم الإنسان وهو هيشتغل
+
+321
+00:23:36,570 --> 00:23:41,260
+ويكون منيح بين 35 و40، لكن إذا ارتفعت بيصير تشوّل
+
+322
+00:23:41,260 --> 00:23:45,740
+شكل الانزيم فيفقد شكله، مش هيقدر يرتبط، وإذا قلت
+
+323
+00:23:45,740 --> 00:23:49,100
+هتقل التصادمات ما بين الانزيم و ال substrate
+
+324
+00:23:49,100 --> 00:23:54,120
+application، ان تطبيقات أخرى مش على جسم الإنسان،
+
+325
+00:23:54,120 --> 00:23:58,200
+مثلا many are a lot lower، بتعيش على درجة حرارة
+
+326
+00:23:58,200 --> 00:24:02,780
+منخفضة، Cold water fish will die at 30°C because
+
+327
+00:24:02,780 --> 00:24:07,340
+their enzyme denaturation، مثلا سمك بيعيش في مياه
+
+328
+00:24:07,340 --> 00:24:13,020
+باردة، لو انت حططيه على درجة حرارة 30°C، هو اصلا
+
+329
+00:24:13,020 --> 00:24:17,500
+جسمه بيعيش في ماء باردة اقل من 30، فانت لو حططيه في
+
+330
+00:24:17,500 --> 00:24:21,380
+30°C، يعني اقل اعلى من الحرارة اللي هو بيعيش فيها،
+
+331
+00:24:21,380 --> 00:24:24,620
+يعني raise temperature، يعني ارتفعت درجة الحرارة،
+
+332
+00:24:24,620 --> 00:24:28,680
+لكن اذا ارتفعت بيصير عنده denaturation لل enzyme،
+
+333
+00:24:29,560 --> 00:24:32,320
+فالسمك اللى بيعيش فى المياه المتجمدة فى المياه
+
+334
+00:24:32,320 --> 00:24:36,480
+الباردة، لو حاططى فى اعلى تلاتين نجالتى على درجة
+
+335
+00:24:36,480 --> 00:24:40,300
+حرارة تلاتين درجة سيليزياس، هيصير عنده denaturation
+
+336
+00:24:40,300 --> 00:24:44,080
+للإنزين، طيب فى عندنا بكتيريا، few bacteria have
+
+337
+00:24:44,080 --> 00:24:47,180
+enzyme very high temperature up to مية درجة
+
+338
+00:24:47,180 --> 00:24:51,310
+سيليزياس، هي أن بعض البكتيريا بتعيش في درجات حرارة
+
+339
+00:24:51,310 --> 00:24:54,830
+عالية بتعيش على 100 درجة سليزياس، فانت لو أخدتها
+
+340
+00:24:54,830 --> 00:24:58,450
+وحطتيها في درجة حرارة قليلة أقل من 100 درجة
+
+341
+00:24:58,450 --> 00:25:01,730
+سليزياس هتقل التصادمات بين الانزيم وال substrate،
+
+342
+00:25:01,730 --> 00:25:06,290
+ويجي نشاط الانزيم عندها، بحكي لـ Most enzymes are
+
+343
+00:25:06,290 --> 00:25:09,930
+fully denaturated at 70 درجة سليزياس، معظم 
+
+344
+00:25:09,930 --> 00:25:13,690
+الانزيمات بيصير اللي هتشوه denaturation على درجة 
+
+345
+00:25:13,690 --> 00:25:18,250
+حرارة 70 درجة سليزياس، أكيد لإن رفعتها عنها أكتر
+
+346
+00:25:18,250 --> 00:25:20,690
+من 40، حينها بيصير denaturation، fully 
+
+347
+00:25:20,690 --> 00:25:26,530
+denaturation على 70 بيكون معظم خلاصة اللي هتشوه
+
+348
+00:25:26,530 --> 00:25:27,930
+بشكل كامل 
diff --git a/PL9fwy3NUQKwZ1Q7zOg1ObgufPMjRI3WY1/yXtOjcfTgyE_raw.json b/PL9fwy3NUQKwZ1Q7zOg1ObgufPMjRI3WY1/yXtOjcfTgyE_raw.json
new file mode 100644
index 0000000000000000000000000000000000000000..e8eb23d9e7e4f8f9a3356609f1019b1a4ed19f09
--- /dev/null
+++ b/PL9fwy3NUQKwZ1Q7zOg1ObgufPMjRI3WY1/yXtOjcfTgyE_raw.json
@@ -0,0 +1 @@
+{"segments": [{"id": 1, "seek": 2593, "start": 0.93, "end": 25.93, "text": "اليوم ده نحكي بموضوع الإنزيمات إيش انهاء الإنزيمات، إيش العوامل اللي بتأثر على الإنزيم اللي بتزيد من سرعة عمل الإنزيم والعوامل المثبتة للإنزيم اللي بتقلل من عمل الإنزيم، اللي بتثبت الإنزيم بيحكي ليه إن all chemical reactions in living organisms require أنزيم", "tokens": [6027, 1829, 20498, 11778, 3224, 8717, 5016, 4117, 1829, 4724, 2304, 2407, 11242, 45367, 33688, 1863, 11622, 32640, 9307, 11933, 1829, 8592, 16472, 3224, 16606, 33688, 1863, 11622, 32640, 9307, 12399, 11933, 1829, 8592, 18863, 2407, 10943, 1211, 13672, 1829, 39894, 10721, 49115, 15844, 33688, 1863, 11622, 32640, 13672, 1829, 39894, 11622, 25708, 9154, 8608, 2288, 27884, 6225, 42213, 33688, 1863, 11622, 32640, 16070, 3615, 2407, 10943, 1211, 9673, 12984, 3555, 2655, 3660, 24976, 28814, 1863, 11622, 32640, 13672, 1829, 39894, 4587, 1211, 1211, 9154, 6225, 42213, 33688, 1863, 11622, 32640, 12399, 13672, 1829, 39894, 12984, 3555, 2655, 33688, 1863, 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0.92041015625}, {"start": 175.54, "end": 175.66, "word": " a", "probability": 0.572265625}, {"start": 175.66, "end": 176.16, "word": " protein", "probability": 0.91015625}, {"start": 176.16, "end": 177.2, "word": " حكينا", "probability": 0.6400146484375}, {"start": 177.2, "end": 177.52, "word": " أنه", "probability": 0.521728515625}, {"start": 177.52, "end": 177.82, "word": " usually", "probability": 0.66455078125}, {"start": 177.82, "end": 178.22, "word": " عادة", "probability": 0.9645182291666666}, {"start": 178.22, "end": 178.8, "word": " الإنزين", "probability": 0.832763671875}, {"start": 178.8, "end": 179.22, "word": " بيكون", "probability": 0.8590494791666666}, {"start": 179.22, "end": 179.64, "word": " عبارة", "probability": 0.9075927734375}, {"start": 179.64, "end": 179.8, "word": " عن", "probability": 0.98291015625}, {"start": 179.8, "end": 180.48, "word": " بروتين", "probability": 0.765869140625}, {"start": 180.48, "end": 181.58, "word": " وglobular", "probability": 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{"start": 188.56, "end": 189.14, "word": " reaction", "probability": 0.9140625}, {"start": 189.14, "end": 189.78, "word": " انه", "probability": 0.45916748046875}, {"start": 189.78, "end": 190.28, "word": " بيعمل", "probability": 0.9439697265625}, {"start": 190.28, "end": 191.22, "word": " كمحفز", "probability": 0.85224609375}, {"start": 191.22, "end": 192.12, "word": " لتسريع", "probability": 0.9908203125}, {"start": 192.12, "end": 192.78, "word": " التفاعل", "probability": 0.931640625}, {"start": 192.78, "end": 193.6, "word": " are", "probability": 0.86767578125}, {"start": 193.6, "end": 194.14, "word": " specific", "probability": 0.94970703125}, {"start": 194.14, "end": 194.58, "word": " for", "probability": 0.96337890625}, {"start": 194.58, "end": 194.94, "word": " what", "probability": 0.96435546875}, {"start": 194.94, "end": 195.26, "word": " they", "probability": 0.93701171875}, {"start": 195.26, "end": 195.62, "word": " will", "probability": 0.8837890625}, {"start": 195.62, 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اللي", "probability": 0.982666015625}, {"start": 226.4, "end": 226.56, "word": " أنا", "probability": 0.86279296875}, {"start": 226.56, "end": 226.8, "word": " بدي", "probability": 0.787109375}, {"start": 226.8, "end": 227.3, "word": " أسرعه،", "probability": 0.8379720052083334}, {"start": 227.3, "end": 227.68, "word": " بيشبه", "probability": 0.9876953125}, {"start": 227.68, "end": 227.84, "word": " ال", "probability": 0.97509765625}, {"start": 227.84, "end": 228.38, "word": " substrate", "probability": 0.87109375}, {"start": 228.38, "end": 229.42, "word": " يعني", "probability": 0.9462890625}, {"start": 229.42, "end": 229.66, "word": " كل", "probability": 0.978515625}, {"start": 229.66, "end": 230.02, "word": " enzyme", "probability": 0.49169921875}, {"start": 230.02, "end": 230.66, "word": " متخصص", "probability": 0.9942626953125}, {"start": 230.66, "end": 231.08, "word": " حاجتنا", "probability": 0.75323486328125}, {"start": 231.08, "end": 231.82, "word": " لتفاعل", "probability": 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substrate اللي هو الـ Saccharose بيروح على الإنزيم المتخصص له بيرتبط في الموقع اللي اسمه active site في الفراغ اللي بالوسط، الإنزيم، الإنزيم هو هذا اللي موقف فيه في الوسط فراغ، بيشبه هذا الفراغ بيشبه مين؟ بيشبه الـ Saccharose، الـ substrate بتيجي هذه الـ Saccharose بترتبط في هذا الموقع، في الـ active site بيكوّن الإنزيم مع substrate complex", "tokens": [5172, 21292, 7435, 1829, 23758, 2423, 39184, 27585, 13672, 1829, 31439, 2423, 39184, 19356, 7374, 541, 4724, 13546, 2407, 5016, 15844, 33688, 1863, 11622, 32640, 9673, 2655, 9778, 9381, 9381, 46740, 4724, 13546, 2655, 3555, 9566, 8978, 9673, 30543, 3615, 13672, 1829, 24525, 2304, 3224, 4967, 3621, 8978, 27188, 2288, 5718, 118, 13672, 1829, 20666, 41779, 9566, 12399, 33688, 1863, 11622, 32640, 12399, 33688, 1863, 11622, 32640, 31439, 23758, 13672, 1829, 3714, 30543, 5172, 8978, 3224, 8978, 2423, 41779, 9566, 6156, 2288, 5718, 118, 12399, 4724, 1829, 8592, 3555, 3224, 23758, 27188, 2288, 5718, 118, 4724, 1829, 8592, 3555, 3224, 3714, 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280.81, "end": 281.15, "word": " site", "probability": 0.896484375}, {"start": 281.15, "end": 282.07, "word": " بيكوّن", "probability": 0.834716796875}, {"start": 282.07, "end": 282.71, "word": " الإنزيم", "probability": 0.929931640625}, {"start": 282.71, "end": 282.99, "word": " مع", "probability": 0.8017578125}, {"start": 282.99, "end": 283.53, "word": " substrate", "probability": 0.95751953125}, {"start": 283.53, "end": 284.19, "word": " complex", "probability": 0.869140625}], "temperature": 1.0}, {"id": 12, "seek": 31342, "start": 285.12, "end": 313.42, "text": "بيصير التفاعل اللي هو عبارة عن تكسير الـ substrates فبتتحول الـ substrates إلى product دخلت و بدأت تتحول الحين ل product، لنواتج نواتج هي مرتبطة في الانزيم، فبنسميه انزيم مع product بعد هي بتتحرر ال product هذه النواتج بتطلع برا الانزيم والانزيم بيطلع زي ما دخل بدون أي تغيير ال product بتتحرر من الانزيم والانزيم بيطلع زي ما هو بدون أي تغيير", "tokens": [3555, 1829, 9381, 13546, 16712, 5172, 995, 30241, 13672, 1829, 31439, 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"word": " product", "probability": 0.92236328125}, {"start": 300.66, "end": 300.92, "word": " هذه", "probability": 0.76953125}, {"start": 300.92, "end": 301.48, "word": " النواتج", "probability": 0.953125}, {"start": 301.48, "end": 302.06, "word": " بتطلع", "probability": 0.8868408203125}, {"start": 302.06, "end": 302.36, "word": " برا", "probability": 0.875244140625}, {"start": 302.36, "end": 303.02, "word": " الانزيم", "probability": 0.947265625}, {"start": 303.02, "end": 303.76, "word": " والانزيم", "probability": 0.893310546875}, {"start": 303.76, "end": 304.3, "word": " بيطلع", "probability": 0.90234375}, {"start": 304.3, "end": 304.58, "word": " زي", "probability": 0.994873046875}, {"start": 304.58, "end": 304.72, "word": " ما", "probability": 0.97509765625}, {"start": 304.72, "end": 305.08, "word": " دخل", "probability": 0.99560546875}, {"start": 305.08, "end": 305.52, "word": " بدون", "probability": 0.837890625}, {"start": 305.52, "end": 305.8, "word": " أي", "probability": 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{"start": 659.14, "end": 659.86, "word": " لما", "probability": 0.51776123046875}, {"start": 659.86, "end": 659.94, "word": " ما", "probability": 0.31005859375}, {"start": 659.94, "end": 660.62, "word": " هيسرع", "probability": 0.876953125}, {"start": 660.62, "end": 661.3, "word": " التفاعل", "probability": 0.96435546875}, {"start": 661.3, "end": 661.9, "word": " إذا", "probability": 0.845947265625}, {"start": 661.9, "end": 662.16, "word": " أجى", "probability": 0.7421875}, {"start": 662.16, "end": 662.52, "word": " انزيم", "probability": 0.8349609375}, {"start": 662.52, "end": 662.9, "word": " تاني", "probability": 0.9786783854166666}, {"start": 662.9, "end": 663.4, "word": " ويرتبط", "probability": 0.879150390625}, {"start": 663.4, "end": 663.58, "word": " بال", "probability": 0.99267578125}, {"start": 663.58, "end": 664.18, "word": " substrate", "probability": 0.88623046875}, {"start": 664.18, "end": 665.06, "word": " هيزمش", "probability": 0.7313232421875}, {"start": 665.06, "end": 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وجود أنزيمين هيسرع التفاعل أكتر ولو جيبت كمان أنزيم تالت مرتبط بsubstrate هيسرع التفاعل أكتر والرابع برضه هيسرع التفاعل أكتر وهكذا", "tokens": [3224, 1211, 39896, 30544, 16712, 5172, 995, 30241, 5551, 3794, 2288, 3615, 5296, 15042, 7251, 30544, 16472, 16254, 14739, 11622, 32640, 36764, 24401, 3714, 43500, 3555, 9566, 4724, 30131, 372, 4404, 34051, 14739, 11622, 32640, 9957, 4032, 28820, 36764, 24401, 3714, 43500, 3555, 9566, 4724, 30131, 372, 4404, 5551, 4117, 25708, 49610, 23328, 14739, 11622, 32640, 9957, 39896, 3794, 2288, 3615, 16712, 5172, 995, 30241, 5551, 4117, 2655, 2288, 4032, 1211, 2407, 10874, 1829, 3555, 2655, 9122, 2304, 7649, 14739, 11622, 32640, 6055, 6027, 2655, 3714, 43500, 3555, 9566, 4724, 30131, 372, 4404, 39896, 3794, 2288, 3615, 16712, 5172, 995, 30241, 5551, 4117, 2655, 2288, 16070, 2288, 16758, 3615, 4724, 43042, 3224, 39896, 3794, 2288, 3615, 16712, 5172, 995, 30241, 5551, 4117, 2655, 2288, 37037, 4117, 15730], "avg_logprob": -0.11309814604464918, 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{"start": 679.98, "end": 680.62, "word": " ولو", "probability": 0.7207845052083334}, {"start": 680.62, "end": 680.94, "word": " جيبت", "probability": 0.938232421875}, {"start": 680.94, "end": 681.2, "word": " كمان", "probability": 0.8951822916666666}, {"start": 681.2, "end": 681.58, "word": " أنزيم", "probability": 0.9451497395833334}, {"start": 681.58, "end": 682.02, "word": " تالت", "probability": 0.9313151041666666}, {"start": 682.02, "end": 682.44, "word": " مرتبط", "probability": 0.8348388671875}, {"start": 682.44, "end": 683.06, "word": " بsubstrate", "probability": 0.951416015625}, {"start": 683.06, "end": 683.58, "word": " هيسرع", "probability": 0.9459228515625}, {"start": 683.58, "end": 684.1, "word": " التفاعل", "probability": 0.9774169921875}, {"start": 684.1, "end": 684.66, "word": " أكتر", "probability": 0.9842529296875}, {"start": 684.66, "end": 685.64, "word": " والرابع", "probability": 0.81524658203125}, {"start": 685.64, "end": 685.96, "word": " برضه", "probability": 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saturation point when all the enzyme molecules are occupied للـ Vmax", "tokens": [42, 44, 39896, 6055, 31747, 1829, 11622, 2423, 39184, 27585, 13672, 31377, 2304, 24976, 2407, 9381, 12610, 30731, 8717, 10859, 9381, 8608, 2288, 27884, 16712, 5172, 995, 30241, 33251, 32640, 1829, 16373, 1829, 591, 44, 39896, 6055, 31747, 1829, 11622, 2423, 39184, 27585, 13672, 31377, 2304, 24976, 2407, 9381, 12610, 30731, 8717, 10859, 9381, 8608, 2288, 27884, 16712, 5172, 995, 30241, 2423, 39184, 5952, 5480, 4663, 5480, 457, 309, 2524, 257, 27090, 935, 562, 439, 264, 24521, 13093, 366, 19629, 24976, 39184, 691, 41167], "avg_logprob": -0.19411764285143684, "compression_ratio": 1.8105263157894738, "no_speech_prob": 0.0, "words": [{"start": 932.83, "end": 933.39, "word": "KM", "probability": 0.51171875}, {"start": 933.39, "end": 933.65, "word": " هي", "probability": 0.85205078125}, {"start": 933.65, "end": 934.41, "word": " تركيز", "probability": 0.98779296875}, {"start": 934.41, "end": 934.63, "word": " 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reaction", "probability": 0.9111328125}, {"start": 952.71, "end": 952.97, "word": " but", "probability": 0.47216796875}, {"start": 952.97, "end": 953.25, "word": " it", "probability": 0.95849609375}, {"start": 953.25, "end": 953.81, "word": " reach", "probability": 0.4638671875}, {"start": 953.81, "end": 954.05, "word": " a", "probability": 0.88623046875}, {"start": 954.05, "end": 954.73, "word": " saturation", "probability": 0.93359375}, {"start": 954.73, "end": 955.35, "word": " point", "probability": 0.97509765625}, {"start": 955.35, "end": 955.93, "word": " when", "probability": 0.7685546875}, {"start": 955.93, "end": 956.37, "word": " all", "probability": 0.9453125}, {"start": 956.37, "end": 956.57, "word": " the", "probability": 0.86376953125}, {"start": 956.57, "end": 956.99, "word": " enzyme", "probability": 0.94775390625}, {"start": 956.99, "end": 957.53, "word": " molecules", "probability": 0.6015625}, {"start": 957.53, "end": 957.89, "word": " are", "probability": 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بيحكي أنه at low substrate concentration the enzyme is not saturated with the substrate and the reaction is not proceeding at maximum velocity whereas when the enzyme is saturated with substrate maximum velocity is observed", "tokens": [41185, 43242, 8315, 3714, 1829, 8592, 26895, 4032, 3714, 9957, 2655, 1863, 13672, 1829, 8032, 2304, 20449, 45340, 9957, 11331, 9566, 14407, 12174, 7649, 11536, 4724, 1829, 5016, 4117, 1829, 14739, 3224, 412, 2295, 27585, 9856, 264, 24521, 307, 406, 25408, 365, 264, 27585, 293, 264, 5480, 307, 406, 41163, 412, 6674, 9269, 9735, 562, 264, 24521, 307, 25408, 365, 27585, 6674, 9269, 307, 13095], "avg_logprob": -0.2311789786273783, "compression_ratio": 1.6294416243654823, "no_speech_prob": 2.384185791015625e-07, "words": [{"start": 988.03, "end": 988.29, "word": "في", "probability": 0.54443359375}, {"start": 988.29, "end": 988.91, "word": " عندنا", "probability": 0.48516845703125}, {"start": 988.91, "end": 989.57, "word": " ميشيل", "probability": 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{"start": 1229.21, "end": 1229.51, "word": " active", "probability": 0.61474609375}, {"start": 1229.51, "end": 1229.97, "word": " site", "probability": 0.8134765625}, {"start": 1229.97, "end": 1230.53, "word": " صار", "probability": 0.8076171875}, {"start": 1230.53, "end": 1230.73, "word": " عليه", "probability": 0.69677734375}, {"start": 1230.73, "end": 1231.21, "word": " تشوي", "probability": 0.80419921875}, {"start": 1231.21, "end": 1231.69, "word": " وصار", "probability": 0.7571614583333334}, {"start": 1231.69, "end": 1232.27, "word": " تدمين", "probability": 0.9178059895833334}, {"start": 1232.27, "end": 1233.03, "word": " فال", "probability": 0.931640625}, {"start": 1233.03, "end": 1233.51, "word": " substrate", "probability": 0.88330078125}, {"start": 1233.51, "end": 1234.11, "word": " molecule", "probability": 0.7861328125}, {"start": 1234.11, "end": 1234.35, "word": " مش", "probability": 0.9853515625}, {"start": 1234.35, "end": 1234.89, "word": " ملائم", "probability": 0.8074951171875}, {"start": 1234.89, "end": 1235.41, "word": " لها", "probability": 0.730712890625}, {"start": 1235.41, "end": 1236.15, "word": " هذا", "probability": 0.9306640625}, {"start": 1236.15, "end": 1236.79, "word": " الشكل", "probability": 0.991943359375}, {"start": 1236.79, "end": 1236.99, "word": " مش", "probability": 0.8310546875}, {"start": 1236.99, "end": 1237.51, "word": " هتقدر", "probability": 0.988525390625}, {"start": 1237.51, "end": 1237.81, "word": " تيجي", "probability": 0.951416015625}, {"start": 1237.81, "end": 1238.39, "word": " ترتبط", "probability": 0.9892578125}, {"start": 1238.39, "end": 1238.77, "word": " مع", "probability": 0.9892578125}, {"start": 1238.77, "end": 1239.51, "word": " الانزين", "probability": 0.7784423828125}], "temperature": 1.0}, {"id": 54, "seek": 127232, "start": 1243.26, "end": 1272.32, "text": "هنا مخطط بيوضّح أن الـ pH، optimum pH للبيبسين، هيله بالأحمر البيبسين القمة اللي هو هين بيشتغل عند تلاتة أعلى اللي عمل لهذا الانزيم عند pH تلاتة لتريبسين أعلى pH اللي هو الأزراج القمة اللي هو أعلى يشير عند تمانية أعلى reaction اللي هو بيكون عند pH تمانية العامل اللي بعدين بيأثر على رشاط الانزيم هو الـ temperature، درجة الحرارة", "tokens": [3224, 8315, 3714, 9778, 9566, 9566, 4724, 1829, 2407, 11242, 11703, 5016, 14739, 2423, 39184, 21677, 12399, 39326, 21677, 24976, 21292, 3555, 3794, 9957, 12399, 39896, 43761, 20666, 10721, 5016, 29973, 2423, 21292, 3555, 3794, 9957, 25062, 46007, 13672, 1829, 31439, 8032, 9957, 4724, 1829, 8592, 2655, 17082, 1211, 43242, 6055, 1211, 9307, 3660, 5551, 3615, 23942, 13672, 1829, 6225, 42213, 46740, 15730, 2423, 7649, 11622, 32640, 43242, 21677, 6055, 1211, 9307, 3660, 5296, 2655, 16572, 3555, 3794, 9957, 5551, 3615, 23942, 21677, 13672, 1829, 31439, 16247, 11622, 2288, 26108, 25062, 46007, 13672, 1829, 31439, 5551, 3615, 23942, 7251, 8592, 13546, 43242, 46811, 7649, 10632, 5551, 3615, 23942, 5480, 13672, 1829, 31439, 4724, 1829, 30544, 43242, 21677, 46811, 7649, 10632, 18863, 10943, 1211, 13672, 1829, 39182, 9957, 4724, 1829, 10721, 49115, 15844, 12602, 8592, 41193, 2423, 7649, 11622, 32640, 31439, 2423, 39184, 4292, 12399, 11778, 47341, 3660, 21542, 2288, 9640, 3660], "avg_logprob": -0.23108552043375216, "compression_ratio": 2.0474452554744524, "no_speech_prob": 1.3709068298339844e-06, "words": [{"start": 1243.26, "end": 1243.72, "word": "هنا", "probability": 0.6302490234375}, {"start": 1243.72, "end": 1244.4, "word": " مخطط", "probability": 0.9510498046875}, {"start": 1244.4, "end": 1245.06, "word": " بيوضّح", "probability": 0.8417154947916666}, {"start": 1245.06, "end": 1245.64, "word": " أن", "probability": 0.4423828125}, {"start": 1245.64, "end": 1245.86, "word": " الـ", "probability": 0.747314453125}, {"start": 1245.86, "end": 1246.22, "word": " pH،", "probability": 0.34661865234375}, {"start": 1246.22, "end": 1246.52, "word": " optimum", "probability": 0.71484375}, {"start": 1246.52, "end": 1247.18, "word": " pH", "probability": 0.99072265625}, {"start": 1247.18, "end": 1249.0, "word": " للبيبسين،", "probability": 0.7879231770833334}, {"start": 1249.0, "end": 1249.18, "word": " هيله", "probability": 0.410888671875}, {"start": 1249.18, "end": 1249.6, "word": " بالأحمر", "probability": 0.9591064453125}, {"start": 1249.6, "end": 1250.14, "word": " البيبسين", "probability": 0.92724609375}, {"start": 1250.14, "end": 1250.66, "word": " القمة", "probability": 0.8935546875}, {"start": 1250.66, "end": 1250.94, "word": " اللي", "probability": 0.5736083984375}, {"start": 1250.94, "end": 1251.14, "word": " هو", "probability": 0.73974609375}, {"start": 1251.14, "end": 1251.46, "word": " هين", "probability": 0.2965087890625}, {"start": 1251.46, "end": 1252.08, "word": " بيشتغل", "probability": 0.9689127604166666}, {"start": 1252.08, "end": 1252.42, "word": " عند", "probability": 0.8994140625}, {"start": 1252.42, "end": 1253.0, "word": " تلاتة", "probability": 0.9364013671875}, {"start": 1253.0, "end": 1253.54, "word": " أعلى", "probability": 0.8746744791666666}, {"start": 1253.54, "end": 1254.0, "word": " اللي", "probability": 0.953125}, {"start": 1254.0, "end": 1254.3, "word": " عمل", "probability": 0.8388671875}, {"start": 1254.3, "end": 1254.64, "word": " لهذا", "probability": 0.5357666015625}, {"start": 1254.64, "end": 1255.1, "word": " الانزيم", "probability": 0.8558349609375}, {"start": 1255.1, "end": 1255.34, "word": " عند", "probability": 0.82470703125}, {"start": 1255.34, "end": 1255.78, "word": " pH", "probability": 0.931640625}, {"start": 1255.78, "end": 1256.36, "word": " تلاتة", "probability": 0.9114990234375}, {"start": 1256.36, "end": 1257.5, "word": " لتريبسين", "probability": 0.7390543619791666}, {"start": 1257.5, "end": 1258.18, "word": " أعلى", "probability": 0.8650716145833334}, {"start": 1258.18, "end": 1258.6, "word": " pH", "probability": 0.8466796875}, {"start": 1258.6, "end": 1258.78, "word": " اللي", "probability": 0.921875}, {"start": 1258.78, "end": 1258.88, "word": " هو", "probability": 0.98876953125}, {"start": 1258.88, "end": 1259.42, "word": " الأزراج", "probability": 0.9085693359375}, {"start": 1259.42, "end": 1259.84, "word": " القمة", "probability": 0.89013671875}, {"start": 1259.84, "end": 1260.08, "word": " اللي", "probability": 0.665283203125}, {"start": 1260.08, "end": 1260.16, "word": " هو", "probability": 0.89208984375}, {"start": 1260.16, "end": 1260.46, "word": " أعلى", "probability": 0.9295247395833334}, {"start": 1260.46, "end": 1260.8, "word": " يشير", "probability": 0.47802734375}, {"start": 1260.8, "end": 1261.02, "word": " عند", "probability": 0.71923828125}, {"start": 1261.02, "end": 1261.48, "word": " تمانية", "probability": 0.87646484375}, {"start": 1261.48, "end": 1262.0, "word": " أعلى", "probability": 0.9532877604166666}, {"start": 1262.0, "end": 1262.56, "word": " reaction", "probability": 0.8876953125}, {"start": 1262.56, "end": 1262.8, "word": " اللي", "probability": 0.892822265625}, {"start": 1262.8, "end": 1262.9, "word": " هو", "probability": 0.986328125}, {"start": 1262.9, "end": 1263.38, "word": " بيكون", "probability": 0.9044596354166666}, {"start": 1263.38, "end": 1263.58, "word": " عند", "probability": 0.92626953125}, {"start": 1263.58, "end": 1264.14, "word": " pH", "probability": 0.99658203125}, {"start": 1264.14, "end": 1264.94, "word": " تمانية", "probability": 0.95703125}, {"start": 1264.94, "end": 1267.26, "word": " العامل", "probability": 0.9658203125}, {"start": 1267.26, "end": 1267.4, "word": " اللي", "probability": 0.98974609375}, {"start": 1267.4, "end": 1267.82, "word": " بعدين", "probability": 0.7060546875}, {"start": 1267.82, "end": 1268.28, "word": " بيأثر", "probability": 0.9031982421875}, {"start": 1268.28, "end": 1268.78, "word": " على", "probability": 0.857421875}, {"start": 1268.78, "end": 1269.64, "word": " رشاط", "probability": 0.7354329427083334}, {"start": 1269.64, "end": 1270.16, "word": " الانزيم", "probability": 0.964111328125}, {"start": 1270.16, "end": 1270.48, "word": " هو", "probability": 0.95166015625}, {"start": 1270.48, "end": 1270.74, "word": " الـ", "probability": 0.6295166015625}, {"start": 1270.74, "end": 1271.14, "word": " temperature،", "probability": 0.70751953125}, {"start": 1271.14, "end": 1271.74, "word": " درجة", "probability": 0.9622395833333334}, {"start": 1271.74, "end": 1272.32, "word": " الحرارة", "probability": 0.9947509765625}], "temperature": 1.0}, {"id": 55, "seek": 129819, "start": 1273.01, "end": 1298.19, "text": "بحكي لدرجة الحرارة اللي بتأثر على معدل الإنزيم optimum temperature أفضل درجة حرارة لعمل الإنزيم ويكون نشيط اللي هو نشاط الإنزيم في عالة نشاطهم الـ human enzyme work at 35 درجة إلى 40 درجة سليزيا اللي هي ال body temperature 37 درجة سليزيا ببعين ال range درجة حرارة جسم الإنسان من 35 إلى 40 درجة سليزيا", "tokens": [49628, 4117, 1829, 5296, 3215, 47341, 3660, 21542, 2288, 9640, 3660, 13672, 1829, 39894, 10721, 49115, 15844, 20449, 3215, 1211, 33688, 1863, 11622, 32640, 39326, 4292, 5551, 5172, 11242, 1211, 11778, 47341, 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الحرارة", "probability": 0.9808349609375}, {"start": 1274.55, "end": 1275.01, "word": " اللي", "probability": 0.927001953125}, {"start": 1275.01, "end": 1275.93, "word": " بتأثر", "probability": 0.9430338541666666}, {"start": 1275.93, "end": 1276.21, "word": " على", "probability": 0.8779296875}, {"start": 1276.21, "end": 1276.71, "word": " معدل", "probability": 0.8567708333333334}, {"start": 1276.71, "end": 1277.27, "word": " الإنزيم", "probability": 0.87890625}, {"start": 1277.27, "end": 1278.03, "word": " optimum", "probability": 0.73779296875}, {"start": 1278.03, "end": 1278.69, "word": " temperature", "probability": 0.92626953125}, {"start": 1278.69, "end": 1279.37, "word": " أفضل", "probability": 0.984130859375}, {"start": 1279.37, "end": 1279.81, "word": " درجة", "probability": 0.9921875}, {"start": 1279.81, "end": 1280.43, "word": " حرارة", "probability": 0.9925537109375}, {"start": 1280.43, "end": 1280.85, "word": " لعمل", "probability": 0.8502604166666666}, {"start": 1280.85, "end": 1281.49, "word": " الإنزيم", "probability": 0.9822998046875}, {"start": 1281.49, "end": 1281.89, "word": " ويكون", "probability": 0.9475911458333334}, {"start": 1281.89, "end": 1282.33, "word": " نشيط", "probability": 0.8040771484375}, {"start": 1282.33, "end": 1282.47, "word": " اللي", "probability": 0.7509765625}, {"start": 1282.47, "end": 1282.59, "word": " هو", "probability": 0.8154296875}, {"start": 1282.59, "end": 1282.89, "word": " نشاط", "probability": 0.9890950520833334}, {"start": 1282.89, "end": 1283.39, "word": " الإنزيم", "probability": 0.908935546875}, {"start": 1283.39, "end": 1283.59, "word": " في", "probability": 0.93017578125}, {"start": 1283.59, "end": 1283.87, "word": " عالة", "probability": 0.6608072916666666}, {"start": 1283.87, "end": 1284.45, "word": " نشاطهم", "probability": 0.98046875}, {"start": 1284.45, "end": 1285.31, "word": " الـ", "probability": 0.699951171875}, {"start": 1285.31, "end": 1285.49, "word": " human", "probability": 0.59765625}, {"start": 1285.49, "end": 1285.93, "word": " enzyme", "probability": 0.90478515625}, {"start": 1285.93, "end": 1286.25, "word": " work", "probability": 0.671875}, {"start": 1286.25, "end": 1286.55, "word": " at", "probability": 0.8740234375}, {"start": 1286.55, "end": 1287.01, "word": " 35", "probability": 0.89111328125}, {"start": 1287.01, "end": 1287.67, "word": " درجة", "probability": 0.8899739583333334}, {"start": 1287.67, "end": 1287.83, "word": " إلى", "probability": 0.9365234375}, {"start": 1287.83, "end": 1288.15, "word": " 40", "probability": 0.98876953125}, {"start": 1288.15, "end": 1288.51, "word": " درجة", "probability": 0.98828125}, {"start": 1288.51, "end": 1288.93, "word": " سليزيا", "probability": 0.58355712890625}, {"start": 1288.93, "end": 1289.17, "word": " اللي", "probability": 0.839599609375}, {"start": 1289.17, "end": 1289.29, "word": " هي", "probability": 0.89794921875}, {"start": 1289.29, "end": 1289.41, "word": " ال", "probability": 0.83154296875}, {"start": 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"word": " at", "probability": 0.9326171875}, {"start": 1507.85, "end": 1508.39, "word": " 70", "probability": 0.90771484375}, {"start": 1508.39, "end": 1508.77, "word": " درجة", "probability": 0.7735188802083334}, {"start": 1508.77, "end": 1509.43, "word": " سليزياس،", "probability": 0.5890299479166666}, {"start": 1509.43, "end": 1509.93, "word": " معظم", "probability": 0.9015299479166666}, {"start": 1509.93, "end": 1510.69, "word": " الانزيمات", "probability": 0.8330078125}, {"start": 1510.69, "end": 1511.05, "word": " بيصير", "probability": 0.908447265625}, {"start": 1511.05, "end": 1511.19, "word": " اللي", "probability": 0.5577392578125}, {"start": 1511.19, "end": 1511.81, "word": " هتشوه", "probability": 0.8744140625}, {"start": 1511.81, "end": 1512.63, "word": " denaturation", "probability": 0.7134602864583334}, {"start": 1512.63, "end": 1513.19, "word": " على", "probability": 0.79248046875}, {"start": 1513.19, "end": 1513.69, "word": " درجة", "probability": 0.98779296875}, {"start": 1513.69, "end": 1514.29, "word": " حرارة", "probability": 0.9908447265625}, {"start": 1514.29, "end": 1514.69, "word": " 70", "probability": 0.8466796875}, {"start": 1514.69, "end": 1515.23, "word": " درجة", "probability": 0.982421875}, {"start": 1515.23, "end": 1516.21, "word": " سليزياس،", "probability": 0.8529866536458334}, {"start": 1516.21, "end": 1516.47, "word": " أكيد", "probability": 0.9208984375}, {"start": 1516.47, "end": 1516.71, "word": " لإن", "probability": 0.7654622395833334}, {"start": 1516.71, "end": 1517.35, "word": " رفعتها", "probability": 0.96240234375}, {"start": 1517.35, "end": 1517.61, "word": " عنها", "probability": 0.858642578125}, {"start": 1517.61, "end": 1518.25, "word": " أكتر", "probability": 0.8145751953125}, {"start": 1518.25, "end": 1518.39, "word": " من", "probability": 0.9921875}, {"start": 1518.39, "end": 1518.73, "word": " 40،", "probability": 0.6082763671875}, {"start": 1518.73, "end": 1519.03, "word": " حينها", "probability": 0.3980712890625}, {"start": 1519.03, "end": 1519.25, "word": " بيصير", "probability": 0.9842529296875}, {"start": 1519.25, "end": 1520.35, "word": " denaturation،", "probability": 0.68865966796875}, {"start": 1520.35, "end": 1520.69, "word": " fully", "probability": 0.7802734375}, {"start": 1520.69, "end": 1521.57, "word": " denaturation", "probability": 0.9205729166666666}, {"start": 1521.57, "end": 1521.85, "word": " على", "probability": 0.8388671875}, {"start": 1521.85, "end": 1522.31, "word": " 70", "probability": 0.958984375}, {"start": 1522.31, "end": 1522.75, "word": " بيكون", "probability": 0.8751627604166666}, {"start": 1522.75, "end": 1523.35, "word": " معظم", "probability": 0.9677734375}, {"start": 1523.35, "end": 1523.99, "word": " خلاصة", "probability": 0.89453125}, {"start": 1523.99, "end": 1524.21, "word": " اللي", "probability": 0.558868408203125}, {"start": 1524.21, "end": 1526.53, "word": " هتشوه", "probability": 0.879345703125}, {"start": 1526.53, "end": 1527.07, "word": " بشكل", "probability": 0.7548828125}, {"start": 1527.07, "end": 1527.93, "word": " كامل", "probability": 0.9763997395833334}], "temperature": 1.0}], "language": "ar", "language_probability": 1.0, "duration": 1529.26625, "duration_after_vad": 1499.0406249999985}
\ No newline at end of file
diff --git a/PL9fwy3NUQKwZ1Q7zOg1ObgufPMjRI3WY1/yXtOjcfTgyE_raw.srt b/PL9fwy3NUQKwZ1Q7zOg1ObgufPMjRI3WY1/yXtOjcfTgyE_raw.srt
new file mode 100644
index 0000000000000000000000000000000000000000..0d3b4b410419430e95577a40c528b19138843448
--- /dev/null
+++ b/PL9fwy3NUQKwZ1Q7zOg1ObgufPMjRI3WY1/yXtOjcfTgyE_raw.srt
@@ -0,0 +1,1392 @@
+1
+00:00:00,930 --> 00:00:05,190
+اليوم ده نحكي بموضوع الإنزيمات إيش انهاء
+
+2
+00:00:05,190 --> 00:00:09,450
+الإنزيمات، إيش العوامل اللي بتأثر على الإنزيم اللي
+
+3
+00:00:09,450 --> 00:00:14,990
+بتزيد من سرعة عمل الإنزيم والعوامل المثبتة للإنزيم
+
+4
+00:00:14,990 --> 00:00:19,990
+اللي بتقلل من عمل الإنزيم، اللي بتثبت الإنزيم
+
+5
+00:00:19,990 --> 00:00:24,270
+بيحكي ليه إن all chemical reactions in living
+
+6
+00:00:24,270 --> 00:00:27,930
+organisms require أنزيمto work for building
+
+7
+00:00:27,930 --> 00:00:32,150
+breaking down molecule or speed up the reaction ان
+
+8
+00:00:32,150 --> 00:00:37,250
+كل التفاعلات في جسم الإنسان تحتاج لوجود إنزين سواء
+
+9
+00:00:37,250 --> 00:00:41,850
+بناء سواء هدم كل هذه for speed up the reaction
+
+10
+00:00:41,850 --> 00:00:47,690
+لتسريع التفاعل في عندنا حاجة اسمها enzyme kinetics
+
+11
+00:00:47,690 --> 00:00:54,660
+enzyme kinetics هي دراسةمعدل حدوث التفاعل to study
+
+12
+00:00:54,660 --> 00:00:59,900
+the rate at which an enzyme works تفاعلات معدل
+
+13
+00:00:59,900 --> 00:01:06,560
+حدوث الانزيم معدل عمل الانزيم الانزيم هو عبارة عن
+
+14
+00:01:06,560 --> 00:01:11,120
+biological catalyst ومحفظ بيولوجي usually a
+
+15
+00:01:11,120 --> 00:01:16,840
+globular protein عادة هو عبارة عن بروتينproduced
+
+16
+00:01:16,840 --> 00:01:21,180
+by living organism يصنع في جسم الكائن الحي for
+
+17
+00:01:21,180 --> 00:01:24,640
+speed up specific chemical reaction without itself
+
+18
+00:01:24,640 --> 00:01:30,080
+destroyed or changed in any way بدون بيسرع التفاعل
+
+19
+00:01:30,080 --> 00:01:35,100
+وظيفته إنه يسرع التفاعل الكيميائي بدون ما يستهلك
+
+20
+00:01:35,100 --> 00:01:40,320
+أو إنه يتدمر أو يصير عليه تغيير في هذا التفاعل إذن
+
+21
+00:01:40,320 --> 00:01:45,440
+هو محفز بيولوجي عادة بيكون عبارة عن البروتينبينتج
+
+22
+00:01:45,440 --> 00:01:49,860
+في أجسام الكائنات الحية لتسريع التفاعل بدون ما
+
+23
+00:01:49,860 --> 00:01:54,380
+يصير عليه استهلاك أو تدمير أو تغيير، إنما بيخرج من
+
+24
+00:01:54,380 --> 00:01:58,520
+التفاعل زي ما دخل. They are reproduced by the
+
+25
+00:01:58,520 --> 00:02:01,280
+living organism and are usually present in only
+
+26
+00:02:01,280 --> 00:02:05,040
+very small amount in various cells، بينتج في أجسام
+
+27
+00:02:05,040 --> 00:02:08,780
+الكائنات الحية وبالعادة بيكون بكميات قليلة.
+
+28
+00:02:11,180 --> 00:02:19,500
+ما هي خصائص الانزيم؟ خصائص الانزيم الانزيم
+
+29
+00:02:19,500 --> 00:02:23,960
+لا يستهلك في التفاعل، بيدخل على التفاعل بيعمل على
+
+30
+00:02:23,960 --> 00:02:28,080
+تسريع التفاعل الكيميائي بدون ما يستهلك في التفاعل،
+
+31
+00:02:28,080 --> 00:02:33,040
+بعد ما يخلص التفاعل، بيطلع منه زي ما دخل انزيم لا
+
+32
+00:02:33,040 --> 00:02:38,120
+يتغير من نفسه، ولا بيسير عليه حتى أي تغيير، زي ما
+
+33
+00:02:38,120 --> 00:02:42,740
+دخله بيطلعreused again for the same reaction with
+
+34
+00:02:42,740 --> 00:02:46,640
+other molecules أقدر أستخدمه مرة تانية لنفس
+
+35
+00:02:46,640 --> 00:02:51,740
+التفاعل مع مادة تانية بستخدمه مرة تانية لنفس
+
+36
+00:02:51,740 --> 00:02:56,160
+التفاعل مع مادة تانية most enzyme are a protein
+
+37
+00:02:56,160 --> 00:02:59,800
+حكينا أنه usually عادة الإنزين بيكون عبارة عن
+
+38
+00:02:59,800 --> 00:03:04,600
+بروتين وglobular protein يعني بيكونtertiary أو
+
+39
+00:03:04,600 --> 00:03:08,560
+equaternal act as a catalyst to accelerate the
+
+40
+00:03:08,560 --> 00:03:13,600
+reaction انه بيعمل كمحفز لتسريع التفاعل are
+
+41
+00:03:13,600 --> 00:03:18,060
+specific for what they will catalyze وهو متخصص
+
+42
+00:03:18,060 --> 00:03:26,540
+لتفاعل معين كل إنزيم متخصص لإنه يسرع تفاعل معينكيف
+
+43
+00:03:26,540 --> 00:03:30,980
+بيشتغل الـ enzyme؟ الـ enzyme بيشتغل إنه عندنا الـ
+
+44
+00:03:30,980 --> 00:03:34,640
+enzyme فيه موقع اسمه الـ active site الـ active
+
+45
+00:03:34,640 --> 00:03:38,600
+site هو موقع نشط بترتبط فيه ال substrate اللي بدنا
+
+46
+00:03:38,600 --> 00:03:43,100
+نسرع إليها التفاعل في موقع في ال enzyme اسمه الـ
+
+47
+00:03:43,100 --> 00:03:47,300
+active site هذا بيشبه الإشي اللي أنا بدي أسرعه،
+
+48
+00:03:47,300 --> 00:03:51,080
+بيشبه ال substrate يعني كل enzyme متخصص حاجتنا
+
+49
+00:03:51,080 --> 00:03:55,670
+لتفاعل معينكيف متخصص إنه فيه موقع «active site»
+
+50
+00:03:55,670 --> 00:03:59,050
+بيشبه المادة اللي بدي أسرها؟ يعني على فرض في هذه
+
+51
+00:03:59,050 --> 00:04:02,190
+المعادلة، في هذه الدوران، إيش عندي؟ على الجنب،
+
+52
+00:04:02,190 --> 00:04:06,830
+بالأخطر، على الشمال، عندي substrate اسمه الساكروز،
+
+53
+00:04:06,830 --> 00:04:10,450
+سكر الساكروز، هذا سكر ثونائي عبارة عن اتحاد
+
+54
+00:04:10,450 --> 00:04:14,470
+جوليكوز والفراكتور، نعمله تكسير، نعمله عملية
+
+55
+00:04:14,470 --> 00:04:19,210
+breakdown عشان نتكون جوليكوز والفراكتورفبيجي هذا
+
+56
+00:04:19,210 --> 00:04:21,470
+الـ substrate اللي هو الـ Saccharose بيروح على
+
+57
+00:04:21,470 --> 00:04:25,970
+الإنزيم المتخصص له بيرتبط في الموقع اللي اسمه
+
+58
+00:04:25,970 --> 00:04:30,230
+active site في الفراغ اللي بالوسط، الإنزيم،
+
+59
+00:04:30,230 --> 00:04:34,150
+الإنزيم هو هذا اللي موقف فيه في الوسط فراغ، بيشبه
+
+60
+00:04:34,150 --> 00:04:36,790
+هذا الفراغ بيشبه مين؟ بيشبه الـ Saccharose، الـ
+
+61
+00:04:36,790 --> 00:04:39,950
+substrate بتيجي هذه الـ Saccharose بترتبط في هذا
+
+62
+00:04:39,950 --> 00:04:42,990
+الموقع، في الـ active site بيكوّن الإنزيم مع
+
+63
+00:04:42,990 --> 00:04:46,900
+substrate complexبيصير التفاعل اللي هو عبارة عن
+
+64
+00:04:46,900 --> 00:04:50,100
+تكسير الـ substrates فبتتحول الـ substrates إلى
+
+65
+00:04:50,100 --> 00:04:54,200
+product دخلت و بدأت تتحول الحين ل product، لنواتج
+
+66
+00:04:54,200 --> 00:04:57,840
+نواتج هي مرتبطة في الانزيم، فبنسميه انزيم مع
+
+67
+00:04:57,840 --> 00:05:02,060
+product بعد هي بتتحرر ال product هذه النواتج بتطلع
+
+68
+00:05:02,060 --> 00:05:06,340
+برا الانزيم والانزيم بيطلع زي ما دخل بدون أي تغيير
+
+69
+00:05:06,340 --> 00:05:11,540
+ال product بتتحرر من الانزيم والانزيم بيطلع زي ما
+
+70
+00:05:11,540 --> 00:05:16,650
+هو بدون أي تغييرعنا المعادلة اللي كيف بيشتغل
+
+71
+00:05:16,650 --> 00:05:20,230
+الانزيم أول حاجة بتيجي انزيم ترتبط مع substrate
+
+72
+00:05:20,230 --> 00:05:23,730
+انزيم زائد substrate بيعطيني انزيم و substrate
+
+73
+00:05:23,730 --> 00:05:27,190
+complex بعدين substrate تحول ل product فبيعطيني
+
+74
+00:05:27,190 --> 00:05:31,810
+انزيم زائد product ال substrate اتحولت إلى نواتر
+
+75
+00:05:31,810 --> 00:05:37,240
+إلى productكيف بيشتغل الانزين مع الـ Substrate؟
+
+76
+00:05:37,240 --> 00:05:41,020
+كيف بيرتبطه؟ حكينا بموقع اسمه الـ ActiveCyan هذا
+
+77
+00:05:41,020 --> 00:05:46,200
+الموقع بس له تركيب مشابه لتركيب الـ Substrate اللي
+
+78
+00:05:46,200 --> 00:05:52,600
+خاص في هذا الانزينعلى مبدأ اسمه look and key
+
+79
+00:05:52,600 --> 00:05:57,200
+hypothesis اللي هو خاصية القفل والمفتاح زي مثلا
+
+80
+00:05:57,200 --> 00:06:01,160
+عندنا لما يكون عندنا جفن القفل هاد بس وإله مفتاح
+
+81
+00:06:01,160 --> 00:06:05,700
+واحد بيفتحه بيدخل فيه وبيقدر يفتحه والإنزيم زي هاد
+
+82
+00:06:05,700 --> 00:06:09,900
+عنده موقع اسمه active site ترتبط معاه substrate
+
+83
+00:06:09,900 --> 00:06:13,820
+متخصصة فيه بس هو إله بيشبهه هذا الموقع اللي بيشبه
+
+84
+00:06:13,820 --> 00:06:17,260
+زي إن هنا الجطعة اللي اللونها زهر هي اللي على
+
+85
+00:06:17,260 --> 00:06:21,690
+الجنبهذا الـ substrate والأصفر الانزين طالع على
+
+86
+00:06:21,690 --> 00:06:24,910
+الانزين من الجنب فيه موقع اسمه الـ Active site
+
+87
+00:06:24,910 --> 00:06:29,210
+بيشبه تركيب الـ substrate بتيجي السبستريات بترتبط
+
+88
+00:06:29,210 --> 00:06:33,750
+في هذا الموقع بيكوّن انزين substrate complex تكون
+
+89
+00:06:33,750 --> 00:06:37,330
+هذا ال complex بيصير التفاعل ان السبستريات هيدا
+
+90
+00:06:37,330 --> 00:06:41,970
+تتحول لبردعبتتحول ل product مرتبطة في الانزيم
+
+91
+00:06:41,970 --> 00:06:46,090
+فبتكون الانزيم و product بعد هيك بيتحرر ال product
+
+92
+00:06:46,090 --> 00:06:50,970
+بتطلع و بيطلع الانزيم زي ما هو زي ما دخل و بيطلع و
+
+93
+00:06:50,970 --> 00:06:57,850
+بقدر استخدمه مرة تانية في تفاعل تانيأجيت أنا عندي
+
+94
+00:06:57,850 --> 00:07:02,330
+تفاعل أي تفاعل كيف بدي أخليه يكون سريع making
+
+95
+00:07:02,330 --> 00:07:06,310
+reaction go faster يعني بدي أسرع التفاعل بيحكيلي
+
+96
+00:07:06,310 --> 00:07:10,230
+عند رفع درجة الحرارة الجزيئات بتتحرك أسرع
+
+97
+00:07:10,230 --> 00:07:12,950
+increasing the temperature make molecule move
+
+98
+00:07:12,950 --> 00:07:17,410
+faster إذا أنا رفعت درجة الحرارة الجزيئات بتتحرك
+
+99
+00:07:17,410 --> 00:07:24,120
+أسرع فالتفاعل هيكون أسرعلكن biological systems are
+
+100
+00:07:24,120 --> 00:07:27,660
+very sensitive to temperature change إن جسم
+
+101
+00:07:27,660 --> 00:07:32,180
+الإنسان حساس للتغير في درجة الحرارة إذا قالت في
+
+102
+00:07:32,180 --> 00:07:35,280
+عندي تفاعل في جسم الإنسان، في داخل جسم الإنسان
+
+103
+00:07:35,280 --> 00:07:39,140
+وهذا التفاعل أنا بدي أسرعلو رفعتي درجة الحرارة
+
+104
+00:07:39,140 --> 00:07:43,340
+هيأثر على جسم الإنسان فإن رفع درجات الحرارة
+
+105
+00:07:43,340 --> 00:07:48,060
+لتفاعلات بتحدث داخل جسم الإنسان بتأثر و تأثير عنها
+
+106
+00:07:48,060 --> 00:07:52,340
+تأثيرات، بينتج عنها تأثيرات فكيف بدي أسرع التفاعل
+
+107
+00:07:52,340 --> 00:07:56,380
+في داخل جسم الإنسان؟ بالإنزيم enzymes can increase
+
+108
+00:07:56,380 --> 00:07:58,760
+the rate of reaction without increasing the
+
+109
+00:07:58,760 --> 00:08:03,500
+temperature استخدم إنزيم بيرفع معدل حدوث التفاعل
+
+110
+00:08:03,500 --> 00:08:09,040
+بدون ما يزود درجة الحرارةwithout increasing the
+
+111
+00:08:09,040 --> 00:08:13,400
+temperature النزيم بيسرع التفاعل بدون زيادة درجة
+
+112
+00:08:13,400 --> 00:08:16,740
+الحرارة طب كيف بيشتغل النزيم؟ بيزودش درجة الحرارة،
+
+113
+00:08:16,740 --> 00:08:21,280
+شو بيسوي؟ بيقلل حاجة بنسميها ال activation energy،
+
+114
+00:08:21,280 --> 00:08:26,480
+اللي هي طاقة التنشيط، بيقلل الطاقة اللازمة لحدوث
+
+115
+00:08:26,480 --> 00:08:32,100
+التفاعللذن الإنزيم بيقلل وجود حدوث التفاعل عن طريق
+
+116
+00:08:32,100 --> 00:08:36,900
+تقليل الطاقة اللازمة وبالتالي بيسرع التفاعل بدون
+
+117
+00:08:36,900 --> 00:08:41,960
+تغيير درجة الحرارة لأنه إذا غيرت درجة الحرارة داخل
+
+118
+00:08:41,960 --> 00:08:45,880
+جسم الإنسان لها تأثيرات ممكن يصير denaturation
+
+119
+00:08:45,880 --> 00:08:50,380
+تدمير للإنزيمات لأن معظم الإنزيمات هي بروتينات
+
+120
+00:08:50,380 --> 00:08:58,010
+فهتتأثر بدرجة الحرارةهنا عندنا رسم، رسم ليه؟ إنه
+
+121
+00:08:58,010 --> 00:09:02,870
+التفاعل كده هيستهلك طاقة لو كان موجود الإنزيم، ولو
+
+122
+00:09:02,870 --> 00:09:05,870
+التفاعل ماشي بدون وجود الإنزيم، كده هياخد طاقة
+
+123
+00:09:05,870 --> 00:09:10,890
+الخط المتقطع، اللي هو قمة أعلى، عند القمة، الخط
+
+124
+00:09:10,890 --> 00:09:15,490
+المتقطع مش مكتوب activation energy without انزعج،
+
+125
+00:09:15,490 --> 00:09:21,360
+إنه هانحدوث التفاعل بدون وجود الانزيم استهلك طاقة
+
+126
+00:09:21,360 --> 00:09:25,200
+أعلى إذا الطاقة اللي استهلاكها التفاعل في عدم وجود
+
+127
+00:09:25,200 --> 00:09:29,480
+الانزيم هتكون أعلى يعني هيمشي التفاعل أبطأ في الخط
+
+128
+00:09:29,480 --> 00:09:33,440
+اللي تحته مكتوب انه activation energy with enzyme
+
+129
+00:09:33,440 --> 00:09:38,540
+جديش استهلك طاقة في وجود الانزيم كانت الطاقة أقل
+
+130
+00:09:38,540 --> 00:09:44,440
+فبنستنتج أن الانزيم يعمل على تقليل الطاقة اللازمة
+
+131
+00:09:44,440 --> 00:09:50,290
+للتفاعلوبسرع التفاعل بدون الحاجة إلى تغيير درجة
+
+132
+00:09:50,290 --> 00:09:55,960
+الحرارةعوامل بتأثر على نشاط الإنزين factor
+
+133
+00:09:55,960 --> 00:10:00,380
+affecting enzyme activity ما هي العوامل اللي بتأثر
+
+134
+00:10:00,380 --> 00:10:04,040
+على نشاط الإنزين؟ في عندنا عدة عوامل أول عامل
+
+135
+00:10:04,040 --> 00:10:07,820
+substrate concentration تركيز ال substrate اللي هي
+
+136
+00:10:07,820 --> 00:10:11,220
+المادة اللي بدأ ترتبط مع الإنزين عشان ينشطها عشان
+
+137
+00:10:11,220 --> 00:10:15,020
+يسرع التفاعل فالتركيز المادة بيأثر على نشاط
+
+138
+00:10:15,020 --> 00:10:20,410
+الإنزينالـ pH درجة الـ pH بتأثر على نشاط الانزين
+
+139
+00:10:20,410 --> 00:10:25,170
+temperature درجة الحرارة بتأثر ال inhibitor اللي
+
+140
+00:10:25,170 --> 00:10:31,730
+هي المثبتات بتثبت عمل الانزين هتأثر أكيد على نشاط
+
+141
+00:10:31,730 --> 00:10:37,130
+الانزين هندرس واحدة واحدة من هدول العوامل نيجي
+
+142
+00:10:37,130 --> 00:10:39,650
+لأول عامل من العوامل اللي هو ال substrate
+
+143
+00:10:39,650 --> 00:10:44,490
+concentration تركيز ال substrate كيف يؤثر على نشاط
+
+144
+00:10:44,490 --> 00:10:49,200
+الانزيناللي جيت لو نعتبر أنه عندي أنزيمات موجودة
+
+145
+00:10:49,200 --> 00:10:53,040
+هاي الانزيم كل انزيم له substrate ييجي الانزيم
+
+146
+00:10:53,040 --> 00:10:56,820
+الهول بيرتبط مع ال substrate في موقع الactive site
+
+147
+00:10:56,820 --> 00:11:01,300
+بيرتبط الانزيم بال substrate لما ما هيسرع التفاعل
+
+148
+00:11:01,300 --> 00:11:05,060
+إذا أجى انزيم تاني ويرتبط بال substrate هيزمش
+
+149
+00:11:05,060 --> 00:11:09,040
+التفاعل أسرع من الأول من واحد لحاله لما يكونوا
+
+150
+00:11:09,040 --> 00:11:12,340
+اتنينهل هيكون التفاعل أسرع لما يكون اندي أنزيم
+
+151
+00:11:12,340 --> 00:11:16,040
+واحد مرتبط بsubstrate أو أنزيمين وكل واحد مرتبط
+
+152
+00:11:16,040 --> 00:11:19,980
+بsubstrate أكيد وجود أنزيمين هيسرع التفاعل أكتر
+
+153
+00:11:19,980 --> 00:11:23,580
+ولو جيبت كمان أنزيم تالت مرتبط بsubstrate هيسرع
+
+154
+00:11:23,580 --> 00:11:28,040
+التفاعل أكتر والرابع برضه هيسرع التفاعل أكتر وهكذا
+
+155
+00:11:28,820 --> 00:11:33,180
+فكل ما زاد الـ substrate concentration تركيز الـ
+
+156
+00:11:33,180 --> 00:11:37,300
+substrate كل ما زاد تركيز الـ substrate هيزيد
+
+157
+00:11:37,300 --> 00:11:42,240
+الإرتباط بين أنزيمه و substrate هيسرع التفاعل أكتر
+
+158
+00:11:42,240 --> 00:11:48,260
+يعني العلاقة ترضية ما بين تركيز ال substrate و
+
+159
+00:11:48,260 --> 00:11:54,420
+سرعة التفاعلكل ما أضيف substrate بتزيد سرعة
+
+160
+00:11:54,420 --> 00:11:59,600
+التفاعل فالعلاقة ترضية the increase in velocity is
+
+161
+00:11:59,600 --> 00:12:03,200
+proportional to the substrate concentration إن
+
+162
+00:12:03,200 --> 00:12:08,020
+سرعة التفاعل زيادة سرعة التفاعل هي علاقة ترضية مع
+
+163
+00:12:08,020 --> 00:12:14,130
+تركيز ال substrateهنا المخطط التاني اللي حكيناه
+
+164
+00:12:14,130 --> 00:12:18,130
+بالأول ان العلاقة ترضي بين تركيز ال substrate
+
+165
+00:12:18,130 --> 00:12:24,340
+وصورة التفاعل الكيمياطيب، هل هذه العلاقة ترضية إلى
+
+166
+00:12:24,340 --> 00:12:28,580
+ما لنهايها تستمر ترضية إن أنا كل ما أضيف substrate
+
+167
+00:12:28,580 --> 00:12:32,660
+هتزيد سرعة التفاعل كل ما أضيف substrate بتزيد سرعة
+
+168
+00:12:32,660 --> 00:12:38,040
+التفاعل؟ لأ، هذه العلاقة إلها حد معين كافي يعني،
+
+169
+00:12:38,040 --> 00:12:42,340
+أنا جيتلى نفرض إن هذا المكان فيه أربع إنزيمات بس،
+
+170
+00:12:42,340 --> 00:12:46,520
+لو أنا أضفت substrate، بيتصبط مع الإنزيم الأول و
+
+171
+00:12:46,520 --> 00:12:51,020
+بيسرع التفاعللو ضفتني substrate تاني بيرتبط مع
+
+172
+00:12:51,020 --> 00:12:55,140
+الانزيم التاني والتفاعل بيمشي أسرع أكتر طب لو ضفت
+
+173
+00:12:55,140 --> 00:12:58,360
+substrate تالت برضه هيرتبط مع الانزيم التالت
+
+174
+00:12:58,360 --> 00:13:03,060
+الموجود ويسرع التفاعل أكتر يعني كل ما أضيف
+
+175
+00:13:03,060 --> 00:13:05,900
+substrate بيرتبط مع الانزيم بيسرع التفاعل بيصير
+
+176
+00:13:05,900 --> 00:13:09,960
+أسرع حتى الآن العلاقة ترضيه طب عندي أخر انزيم Dial
+
+177
+00:13:09,960 --> 00:13:14,020
+الرابع لو ضفت عليه substrate هيرتبط مع الانزيم
+
+178
+00:13:14,020 --> 00:13:17,830
+ويسرع التفاعل أسرعهلقيت؟ خلصوا الانزيمات
+
+179
+00:13:17,830 --> 00:13:22,670
+الموجودات، كل انزيم صار مرتبط ب substrate و سرعوا
+
+180
+00:13:22,670 --> 00:13:26,950
+التفاعل، هلقيت؟ أنا لو ضفت substrate خامس، هلفي
+
+181
+00:13:26,950 --> 00:13:30,890
+انزيم إنه ييجي يرتبط فيه هذا ال substrate؟ لأ، فال
+
+182
+00:13:30,890 --> 00:13:33,670
+substrate اللي أنا ضفته، هلقيت؟ الخامس مشيله
+
+183
+00:13:33,670 --> 00:13:38,540
+انزيم، فمش هيرتبط ولا هيمشي التفاعل أسرعالجد فانا
+
+184
+00:13:38,540 --> 00:13:43,680
+خلاص ان كل الانزيمات صارت متشبعة كل انزيم مرتبط ب
+
+185
+00:13:43,680 --> 00:13:47,720
+substrate فمهما أضيف substrate مش هيلاجي انزيم
+
+186
+00:13:47,720 --> 00:13:51,600
+يرتبط فيه فهنا أنا بكون وصلت لنقطة اسمها
+
+187
+00:13:51,600 --> 00:13:57,000
+saturation pointSaturation point هي نقطة التشبع
+
+188
+00:13:57,000 --> 00:14:02,280
+اللى عندها بتكون كل الانزيمات مرتبطة بالsubstrate
+
+189
+00:14:02,280 --> 00:14:06,160
+فانا لو ضفت substrate جديد مش هيلاقي انزيم يرتبط
+
+190
+00:14:06,160 --> 00:14:10,720
+فيه فمش هيسرها التفاعل فخلاص عند الانزيم الرابع كل
+
+191
+00:14:10,720 --> 00:14:14,980
+الانزيمات مرتبطة هيأقصى سرعة للتفاعل الكيميائي
+
+192
+00:14:14,980 --> 00:14:22,090
+نسميها VmaxVmax هي أقصى سرعة يصل إليها التفاعل
+
+193
+00:14:22,090 --> 00:14:26,310
+الكيميائي هذه الـ maximum velocity هي أقصى سرعة
+
+194
+00:14:26,310 --> 00:14:30,290
+بيصل إليها التفاعل الكيميائي عند هذه الـ Vmax
+
+195
+00:14:30,290 --> 00:14:34,650
+بتكون كل الإنزيمات مرتبطة بـ substrate ومسارعة
+
+196
+00:14:34,650 --> 00:14:39,170
+للتفاعل بتكون كلها متشبعة كل الإنزيمات saturation
+
+197
+00:14:39,170 --> 00:14:43,410
+with substrate كل إنزيم مرتبط بـ substrate مهما
+
+198
+00:14:43,410 --> 00:14:47,290
+أضيف substrate مش هلاجي إنزيم مرتبط فيهفخلاص مش
+
+199
+00:14:47,290 --> 00:14:50,910
+هيأثر على سرعته التفاعل إيش بده يعمل في التفاعل؟
+
+200
+00:14:50,910 --> 00:14:54,830
+مافيش إنزيم يرتبط فيه، مش هيأثر أنا عندما تتشبع كل
+
+201
+00:14:54,830 --> 00:14:59,710
+إنزيم substrate مهما أضيف substrate مش هيأثر على
+
+202
+00:14:59,710 --> 00:15:04,030
+سرعته التفاعل الكيميائي فكون أنا وصلت لحاجة اسمها
+
+203
+00:15:04,030 --> 00:15:09,590
+الـ Vmaxالـ maximum velocity هي أقصى سرعة يصل
+
+204
+00:15:09,590 --> 00:15:14,350
+إليها التفاعل الكيميائي و عندها بتكون كل الإنزيمات
+
+205
+00:15:14,350 --> 00:15:19,470
+متشبعة مرتبطة بـ substrate هل جيت بيحكيلي عن نص
+
+206
+00:15:19,470 --> 00:15:24,710
+Vmax نص Vmax نص السرعة القصوة هاي يعني نصها في
+
+207
+00:15:24,710 --> 00:15:31,830
+حاجة اسمها KM يعني نص Vmax في حاجة اسمها KM إيش هي
+
+208
+00:15:31,830 --> 00:15:38,510
+KM؟KM هي تركيز الـ substrate اللازم للوصول إلى نُص
+
+209
+00:15:38,510 --> 00:15:44,530
+سرعة التفاعل الكيميائي KM هي تركيز الـ substrate
+
+210
+00:15:44,530 --> 00:15:50,230
+اللازم للوصول إلى نُص سرعة التفاعل الـ factor
+
+211
+00:15:50,230 --> 00:15:54,730
+reaction faster reaction but it reach a saturation
+
+212
+00:15:54,730 --> 00:15:58,730
+point when all the enzyme molecules are occupied
+
+213
+00:15:58,730 --> 00:16:03,280
+للـ Vmaxبحكي لأن العلاقة ترضية صحيح بين
+
+214
+00:16:03,280 --> 00:16:06,620
+الـSubstration، بين الـSubstrate، Concentration،
+
+215
+00:16:06,620 --> 00:16:10,700
+بين تركيز الـSubstrate وسرعة التفاعل، إنه هيسرع
+
+216
+00:16:10,700 --> 00:16:15,700
+التفاعل، ولكن لعند متى الوصول إلى نقطة
+
+217
+00:16:15,700 --> 00:16:20,280
+الـSaturation Point، نقطة التشبط، عندها بتكون كل
+
+218
+00:16:20,280 --> 00:16:25,600
+الإنزيمات مرتبطة بـSubstrate، فبتكون، بنسميها،
+
+219
+00:16:25,600 --> 00:16:31,270
+«High V Map»في عندنا ميشيل و مينتن اللي هم معالمين
+
+220
+00:16:31,270 --> 00:16:34,470
+حطوا قانون بيحكي أنه at low substrate
+
+221
+00:16:34,470 --> 00:16:37,970
+concentration the enzyme is not saturated with the
+
+222
+00:16:37,970 --> 00:16:41,430
+substrate and the reaction is not proceeding at
+
+223
+00:16:41,430 --> 00:16:45,010
+maximum velocity whereas when the enzyme is
+
+224
+00:16:45,010 --> 00:16:48,410
+saturated with substrate maximum velocity is
+
+225
+00:16:48,410 --> 00:16:53,160
+observedعايش ملخص هذا الحكي أنه لما يكون تركيز ال
+
+226
+00:16:53,160 --> 00:16:57,260
+substrate قليل الإنزيمات بتكونش متشبعة بال
+
+227
+00:16:57,260 --> 00:17:00,860
+substrate بتكونش كل الإنزيمات مرتبطة بال substrate
+
+228
+00:17:00,860 --> 00:17:05,420
+مهما كل substrate بيرتبط مع أنزيم ولكن غير متشبع
+
+229
+00:17:05,420 --> 00:17:09,310
+لأن تركيز ال substrate لسه قليلفي هذه الحالة مش
+
+230
+00:17:09,310 --> 00:17:13,090
+هنوصل للـ maximum velocity إلى أقصى سرعة للتفاعل
+
+231
+00:17:13,090 --> 00:17:17,930
+مش هنوصلها بينما لما تكون الانزيمات متشبعة بال
+
+232
+00:17:17,930 --> 00:17:21,190
+substrate خلاص كل الانزيمات مرتبطة بال substrate
+
+233
+00:17:21,190 --> 00:17:25,510
+فأنا باصل لـ maximum velocity اللي عندها بتكون
+
+234
+00:17:25,510 --> 00:17:31,110
+نقطة تتشبع ليه الـ saturated pointعشان هيك العلاقة
+
+235
+00:17:31,110 --> 00:17:35,370
+طردية ما بين تركيز الـsubstrate وسرعة التفاعل
+
+236
+00:17:35,370 --> 00:17:39,210
+الكيميائي لكنها مش علاقة طردية لما لا نهاية ولا
+
+237
+00:17:39,210 --> 00:17:43,910
+انما وصولها إلى نقطة الـsaturated point إلى نقطة
+
+238
+00:17:43,910 --> 00:17:49,390
+التشبع بيثبت المنحنة وتبطل العلاقة طردية بيصيرش كل
+
+239
+00:17:49,390 --> 00:17:55,030
+ما يزيد تركيز الـsubstrate انه يزيد سرعة التفاعل
+
+240
+00:17:56,370 --> 00:18:00,070
+هنا عندنا القانون «ميشيلز أنمينتين إكوايشن» هذا
+
+241
+00:18:00,070 --> 00:18:03,590
+القانون مش مطلوب منها بس ضروري أننا نعرفه الـKM
+
+242
+00:18:03,590 --> 00:18:08,630
+اللي هي تركيز السبسترات عند نص Vmax الـKM حكينا
+
+243
+00:18:08,630 --> 00:18:13,990
+عنها قبل هيك اللي هي تركيز السبسترات عند نص Vmax
+
+244
+00:18:13,990 --> 00:18:17,870
+عامل التاني من العوامل اللي بتأثر على نشاط الإنزيم
+
+245
+00:18:17,870 --> 00:18:21,770
+هو الـpH الـpH بيحكي الـextreme pH level
+
+246
+00:18:21,770 --> 00:18:26,180
+والـdenaturated enzymeالـ pH التغيير في الـ pH
+
+247
+00:18:26,180 --> 00:18:31,080
+بيعمل denaturation للإنزيم بيعمل تشوّه لشكل
+
+248
+00:18:31,080 --> 00:18:35,080
+الإنزيم بيعمل denaturation يعني تشوّه لشكل الإنزيم
+
+249
+00:18:35,080 --> 00:18:40,060
+معظم إنزيمات اللي في جسم الإنسان بتشتغل عند pH من
+
+250
+00:18:40,060 --> 00:18:45,160
+6 إلى 8 most human enzyme work at pH من 6 إلى 80
+
+251
+00:18:45,160 --> 00:18:49,240
+بناء على عش أنه هي من 6 إلى 8depend on its
+
+252
+00:18:49,240 --> 00:18:52,780
+location in the body في إنزيمات برضه بتختلف،
+
+253
+00:18:52,780 --> 00:18:57,320
+بتشتغلش من 6 ل 8، بناء على موقعها في جسم الإنسان،
+
+254
+00:18:57,320 --> 00:19:00,940
+وين هي موجودة، في أي مكان زي مثلا الـPepsin، هذا
+
+255
+00:19:00,940 --> 00:19:05,120
+موجود في الـstomach، في المعدة، ولمّا المعدة وسطها
+
+256
+00:19:05,120 --> 00:19:08,860
+حمضي، فهو بيشتغل على pH زي المكان اللي هو فيه،
+
+257
+00:19:08,860 --> 00:19:13,120
+بيعمل على pH 3الـ Trypsin موجود في الـ small
+
+258
+00:19:13,120 --> 00:19:17,140
+intestine في الأمعاء والأمعاء وسطها قاعدة فهذا
+
+259
+00:19:17,140 --> 00:19:21,040
+بيشتغل عند pH 8 زي المكان اللي هو موجود فيه
+
+260
+00:19:21,040 --> 00:19:24,880
+فالإنزيمات الموجودة في الـ GSM تشتغل عند pH حسب
+
+261
+00:19:24,880 --> 00:19:29,850
+المكان اللي هي موجودة فيه معظمها عند 6 لـ 8طب لو
+
+262
+00:19:29,850 --> 00:19:33,130
+اتغيرت ال pH لهذا الانزيم يعني مثلا ال بيبسين
+
+263
+00:19:33,130 --> 00:19:36,630
+بيشتغل عند تلاتة لو حطته على تمانية يشتغل عند pH
+
+264
+00:19:36,630 --> 00:19:40,370
+تمانية بيحكي اللي بيصير تشوه في شكل الانزيم بيصير
+
+265
+00:19:40,370 --> 00:19:43,690
+عليه denaturation فال structure of انزيم is
+
+266
+00:19:43,690 --> 00:19:48,190
+changed شكل الانزيم وتركيبته هتختلف لما يصير تشوه
+
+267
+00:19:48,190 --> 00:19:53,150
+للانزيم تركيب هذا الانزيم ممكن يختلف، هيختلفإيش
+
+268
+00:19:53,150 --> 00:19:55,910
+ممكن يختلف فيه؟ الـ Active site موقع الـ Active
+
+269
+00:19:55,910 --> 00:19:59,930
+site حكينا هذا ملائم للـ Substrate شكله زي شكل الـ
+
+270
+00:19:59,930 --> 00:20:02,190
+Substrate بتيجي ال Substrate بترتبط في هذا الـ
+
+271
+00:20:02,190 --> 00:20:06,890
+Active site إذا تغيرت الـ pH بيصير فينا تشوة فشكل
+
+272
+00:20:06,890 --> 00:20:11,490
+الـ Active site هذا مع التشوة هيتغير هيبطل ملائم
+
+273
+00:20:11,490 --> 00:20:14,410
+لل Substrate بتيجي ال Substrate ده ترتبط مع
+
+274
+00:20:14,410 --> 00:20:18,250
+الانزيم في موقع الـ Active site بتلاقي بتقدرش
+
+275
+00:20:18,250 --> 00:20:21,850
+ترتبط فيه لأنه مش ملائم لشكلها بتقدرش تيجي ترتبط
+
+276
+00:20:21,850 --> 00:20:25,510
+فيهالـ active site is distorted and the substrate
+
+277
+00:20:25,510 --> 00:20:29,510
+molecule will no longer fit in it أنه ال active
+
+278
+00:20:29,510 --> 00:20:33,510
+site صار عليه تشوي وصار تدمين فال substrate
+
+279
+00:20:33,510 --> 00:20:37,810
+molecule مش ملائم لها هذا الشكل مش هتقدر تيجي
+
+280
+00:20:37,810 --> 00:20:39,510
+ترتبط مع الانزين
+
+281
+00:20:43,260 --> 00:20:49,000
+هنا مخطط بيوضّح أن الـ pH، optimum pH للبيبسين،
+
+282
+00:20:49,000 --> 00:20:52,420
+هيله بالأحمر البيبسين القمة اللي هو هين بيشتغل عند
+
+283
+00:20:52,420 --> 00:20:56,360
+تلاتة أعلى اللي عمل لهذا الانزيم عند pH تلاتة
+
+284
+00:20:56,360 --> 00:21:00,160
+لتريبسين أعلى pH اللي هو الأزراج القمة اللي هو
+
+285
+00:21:00,160 --> 00:21:03,380
+أعلى يشير عند تمانية أعلى reaction اللي هو بيكون
+
+286
+00:21:03,380 --> 00:21:09,640
+عند pH تمانية العامل اللي بعدين بيأثر على رشاط
+
+287
+00:21:09,640 --> 00:21:13,850
+الانزيم هو الـ temperature، درجة الحرارةبحكي لدرجة
+
+288
+00:21:13,850 --> 00:21:18,030
+الحرارة اللي بتأثر على معدل الإنزيم optimum
+
+289
+00:21:18,030 --> 00:21:21,890
+temperature أفضل درجة حرارة لعمل الإنزيم ويكون
+
+290
+00:21:21,890 --> 00:21:25,490
+نشيط اللي هو نشاط الإنزيم في عالة نشاطهم الـ human
+
+291
+00:21:25,490 --> 00:21:29,290
+enzyme work at 35 درجة إلى 40 درجة سليزيا اللي هي
+
+292
+00:21:29,290 --> 00:21:34,310
+ال body temperature 37 درجة سليزيا ببعين ال range
+
+293
+00:21:34,310 --> 00:21:38,190
+درجة حرارة جسم الإنسان من 35 إلى 40 درجة سليزيا
+
+294
+00:21:39,320 --> 00:21:43,900
+بيعمل الانزين بيكون optimum temperature إليه بيكون
+
+295
+00:21:43,900 --> 00:21:48,420
+أعلى نشاط للانزين طب لو ارتفعت درجة الحرارة ريز ال
+
+296
+00:21:48,420 --> 00:21:52,500
+temperature أعلى من 40 درجة سليزيا بيصير عندنا
+
+297
+00:21:52,500 --> 00:21:56,800
+denaturation للانزين لأنه أحكي إن بعض الانزين ماتي
+
+298
+00:21:56,800 --> 00:21:59,140
+عبارة عن البروتين بيصير الـdenaturation
+
+299
+00:21:59,140 --> 00:22:03,110
+الـdenaturation يعني اتشوهيعني تركيب اختلاف unfold
+
+300
+00:22:03,110 --> 00:22:07,930
+تركيب تختلفات lose shape هيفقد شكله إذا شكل الـ
+
+301
+00:22:07,930 --> 00:22:12,090
+active site مش هيكون ملائم لل substrate طيب لو
+
+302
+00:22:12,090 --> 00:22:15,950
+lower temperature لو قلة درجة الحرارة أقل من 35
+
+303
+00:22:15,950 --> 00:22:22,030
+المركبات هتكون بتتحرك ببطء molecules move slower
+
+304
+00:22:22,030 --> 00:22:28,010
+هتتحرك المركبات ببطء بتكون تمشي بشكل باطينوبالتالي
+
+305
+00:22:28,010 --> 00:22:32,870
+التصادمات ما بين الـ substrate والإنزيم هتجل هيكون
+
+306
+00:22:32,870 --> 00:22:37,410
+لأن المراكبات بتتحرك ببطء فهي بتتحرك و تروح على
+
+307
+00:22:37,410 --> 00:22:42,110
+الإنزيم فلما تروح تتحرك ببطء هتقل التصادمات بينها
+
+308
+00:22:42,110 --> 00:22:45,970
+وبين الإنزيم هيقل التقاء ال substrate مع الإنزيم
+
+309
+00:22:45,970 --> 00:22:50,030
+فمش هيرتبطوا مع بعض فبتجل سرعات التفاعل الكيميائي
+
+310
+00:22:50,030 --> 00:22:54,390
+ويجل نشاط الإنزيم إذا درجة الحرارة بتأثر إذا
+
+311
+00:22:54,390 --> 00:22:58,990
+ارتفعت بيصيب دي نات يوراجينإذا قلت، بتقل التصادمات
+
+312
+00:22:58,990 --> 00:23:02,050
+ما بين الـ substrate والإنزيم optimum temperature
+
+313
+00:23:02,050 --> 00:23:05,870
+بيعمل عليها الجسم عليها الهيمينزيمات هي زي درجة
+
+314
+00:23:05,870 --> 00:23:12,710
+حرارة الجسم ما هي ما بين 35 و40 درجة سيليزياهنا
+
+315
+00:23:12,710 --> 00:23:16,070
+عندنا مخطط رسم لـ temperature ونشاط الانزيم
+
+316
+00:23:16,070 --> 00:23:19,350
+temperature and enzyme activity in human زي ما
+
+317
+00:23:19,350 --> 00:23:22,770
+حكينا أنه أعلى نشاط للانزيم optimum temperature
+
+318
+00:23:22,770 --> 00:23:26,470
+for enzyme activity عند القمة أعلى إشي بيكون عند
+
+319
+00:23:26,470 --> 00:23:31,850
+37 درجة سليزيا عند هذه الدرجة بتكون الأعلى نشاط
+
+320
+00:23:31,850 --> 00:23:36,570
+للانزيم نفس درجة حرارة جسم الإنسان وهو هيشتغل
+
+321
+00:23:36,570 --> 00:23:41,260
+ويكون منيح بين 35 و40لكن إذا ارتفعت بيصير تشوّل
+
+322
+00:23:41,260 --> 00:23:45,740
+شكل الانزيم فيفقد شكله مش هيقدر يرتبط وإذا قلت
+
+323
+00:23:45,740 --> 00:23:49,100
+هتقل التصادمات ما بين الانزيم و ال substrate
+
+324
+00:23:49,100 --> 00:23:54,120
+application ان تطبيقات أخرى مش على جسم الإنسان
+
+325
+00:23:54,120 --> 00:23:58,200
+مثلا many are a lot lower بتعيش على درجة حرارة
+
+326
+00:23:58,200 --> 00:24:02,780
+منخفضةCold water fish will die at 30°C because
+
+327
+00:24:02,780 --> 00:24:07,340
+their enzyme denaturation مثلا سمك بيعيش في مياه
+
+328
+00:24:07,340 --> 00:24:13,020
+باردة لو انت حططيه على درجة حرارة 30°C هو اصلا
+
+329
+00:24:13,020 --> 00:24:17,500
+جسمه بيعيش في ماء باردة اقل من 30 فانت لو حططيه في
+
+330
+00:24:17,500 --> 00:24:21,380
+30°C يعني اقل اعلى من الحرارة اللي هو بيعيش فيها
+
+331
+00:24:21,380 --> 00:24:24,620
+يعني raise temperature يعني ارتفعت درجة الحرارة
+
+332
+00:24:24,620 --> 00:24:28,680
+لكن اذا ارتفعت بيصير عنده denaturation لل enzyme
+
+333
+00:24:29,560 --> 00:24:32,320
+فالسمك اللى بيعيش فى المياه المتجمدة فى المياه
+
+334
+00:24:32,320 --> 00:24:36,480
+الباردة لو حاططى فى اعلى تلاتين نجالتى على درجة
+
+335
+00:24:36,480 --> 00:24:40,300
+حرارة تلاتين درجة سيليزياس هيصير عنده denaturation
+
+336
+00:24:40,300 --> 00:24:44,080
+للإنزين طيب فى عندنا بكتيريا few bacteria have
+
+337
+00:24:44,080 --> 00:24:47,180
+enzyme very high temperature up to مية درجة
+
+338
+00:24:47,180 --> 00:24:51,310
+سيليزياسهي أن بعض البكتيريا بتعيش في درجات حرارة
+
+339
+00:24:51,310 --> 00:24:54,830
+عالي بتعيش على 100 درجة سليزياس فانت لو أخدتها
+
+340
+00:24:54,830 --> 00:24:58,450
+وحطتيها في درجة حرارة قليلة أقل من 100 درجة
+
+341
+00:24:58,450 --> 00:25:01,730
+سليزياس هتقل التصادمات بين الانزيم وال substrate
+
+342
+00:25:01,730 --> 00:25:06,290
+ويجي نشاط الانزيم عندهابحكي لـ Most enzymes are
+
+343
+00:25:06,290 --> 00:25:09,930
+fully denaturated at 70 درجة سليزياس، معظم
+
+344
+00:25:09,930 --> 00:25:13,690
+الانزيمات بيصير اللي هتشوه denaturation على درجة
+
+345
+00:25:13,690 --> 00:25:18,250
+حرارة 70 درجة سليزياس، أكيد لإن رفعتها عنها أكتر
+
+346
+00:25:18,250 --> 00:25:20,690
+من 40، حينها بيصير denaturation، fully
+
+347
+00:25:20,690 --> 00:25:26,530
+denaturation على 70 بيكون معظم خلاصة اللي هتشوه
+
+348
+00:25:26,530 --> 00:25:27,930
+بشكل كامل
+
diff --git a/PL9fwy3NUQKwZ1Q7zOg1ObgufPMjRI3WY1/zJp2OlvRd8A.srt b/PL9fwy3NUQKwZ1Q7zOg1ObgufPMjRI3WY1/zJp2OlvRd8A.srt
new file mode 100644
index 0000000000000000000000000000000000000000..1590a183b2cd414f6588c3b45a0ce74a4604f00a
--- /dev/null
+++ b/PL9fwy3NUQKwZ1Q7zOg1ObgufPMjRI3WY1/zJp2OlvRd8A.srt
@@ -0,0 +1,938 @@
+1
+00:00:03,620 --> 00:00:06,680
+أو وين بيدوب وين بيكون soluble وين بيكون 
+
+2
+00:00:06,680 --> 00:00:09,900
+precipitate يذا فاكرين لما حكينا عن الـ ionization
+
+3
+00:00:09,900 --> 00:00:13,060
+عن الـ amino acid كيف بيتصرف في الأوساط المختلفة
+
+4
+00:00:13,060 --> 00:00:16,480
+لما حاطنا فيه alkaline و فيه neutral و فيه acidic
+
+5
+00:00:16,480 --> 00:00:21,000
+pHنفسها للبروتين، لأن البروتين هو عبارة عن سلسلة
+
+6
+00:00:21,000 --> 00:00:24,320
+من الأحماض الأمينية أنا لو جبت بروتين وحطيته في
+
+7
+00:00:24,320 --> 00:00:28,140
+الـ neutral pH الـ neutral pH حكينا شو بتكون الـ
+
+8
+00:00:28,140 --> 00:00:31,220
+net charge في الأمين واسد، بتكون Zero الـ net
+
+9
+00:00:31,220 --> 00:00:35,240
+charge Zero يعني عدد الشحنات الموجبة بتساوي عدد
+
+10
+00:00:35,240 --> 00:00:39,240
+الشحنات السالبة، فإذا كان عدد الشحنات فيها موجبة
+
+11
+00:00:39,240 --> 00:00:43,020
+وفيها سالبة، بتكون في الـ neutral pHفي شحنات موجبة
+
+12
+00:00:43,020 --> 00:00:48,500
+وفي شحنات سالبة الشحنات المختلفة تتجادب مع بعضها
+
+13
+00:00:48,500 --> 00:00:52,240
+معروف عن الشحنات المختلفة أنها تتجادب و الشحنات
+
+14
+00:00:52,240 --> 00:00:56,300
+المتشابهة تتنافس في الـ neutral بيكون فيه موجب و
+
+15
+00:00:56,300 --> 00:01:00,980
+فيه سالب فبيتجادبوا مع بعض بسبب aggregation أو
+
+16
+00:01:00,980 --> 00:01:05,450
+precipitationفإذا الوين هيترسّب البروتين عند الـ
+
+17
+00:01:05,450 --> 00:01:08,690
+neutral pH اللي هي بتكون فيها الـ pH عند الـ
+
+18
+00:01:08,690 --> 00:01:12,950
+isoelectric point بتساوي الـ isoelectric point
+
+19
+00:01:12,950 --> 00:01:17,330
+فنقطة الـ isoelectric point هيترسّب البروتين لكن
+
+20
+00:01:17,330 --> 00:01:22,410
+مثلا لو حطيته في acidic media يعني الـ pH كانت أقل
+
+21
+00:01:22,410 --> 00:01:27,100
+من الـ isoelectric point acidic يعني pH أقل من الـ
+
+22
+00:01:27,100 --> 00:01:31,300
+isoelectric point حكينا الشحنات بتكون موجبة بتكون
+
+23
+00:01:31,300 --> 00:01:35,260
+الـ net charge موجبة في الـ acidic الـ net charge
+
+24
+00:01:35,260 --> 00:01:38,840
+بتكون موجبة الأحماض الأمينية المكونة للبروتين هتكون
+
+25
+00:01:38,840 --> 00:01:43,010
+شحنتها موجبة يعني شحنات متشابهة positive charge
+
+26
+00:01:43,010 --> 00:01:47,370
+الشحنات المتشابهة إيش هي بيصير بينها؟ بيصير تنافر
+
+27
+00:01:47,370 --> 00:01:52,550
+تنافر و بتبعد عن بعضها و بتتوزع فيه المحلول فبتصير
+
+28
+00:01:52,550 --> 00:01:56,310
+soluble ما بتترسب و انما بيصير soluble طب لو
+
+29
+00:01:56,310 --> 00:02:01,250
+حطيتها في alkaline ميلياجيبت هذا البروتين مكونة من
+
+30
+00:02:01,250 --> 00:02:04,770
+أحمض أمينية حاطته في الـAlkaline يعني الـBH أعلى
+
+31
+00:02:04,770 --> 00:02:08,990
+من الـBi الـBH بتكون أعلى من الـBi على الـAlkaline
+
+32
+00:02:08,990 --> 00:02:12,950
+لإن الـBhi نتش هاجحة كده بتكون في الـAlkaline
+
+33
+00:02:12,950 --> 00:02:16,770
+نيجاتيف فكل الشحنات اللي على أحمض الأمينية نيجاتيف
+
+34
+00:02:16,770 --> 00:02:21,710
+شحنات متشابهة، إيش بيصير بينها؟ بتتنافر، بتتنافر و
+
+35
+00:02:21,710 --> 00:02:26,290
+بتتوزع فيه المحلول وبالتالي بتصير soluble، بتكون
+
+36
+00:02:26,290 --> 00:02:30,800
+soluble مش ابتتجادالو جينا قرا عن هذه النقاط نفس
+
+37
+00:02:30,800 --> 00:02:34,780
+اللي حكيناها تحكيلي الـ solution pH is above the PI
+
+38
+00:02:34,780 --> 00:02:38,780
+الـ pH أعلى من الـ PI مدام الـ pH أعلى يعني أنا وصلت
+
+39
+00:02:38,780 --> 00:02:42,300
+لـ alkaline media في الـ alkaline الـ charge، الـ net
+
+40
+00:02:42,300 --> 00:02:44,260
+charge بتكون negatively charged
+
+41
+00:02:49,890 --> 00:02:53,510
+الشحنات المتشابهة لـ like charge عندها قوة تنافر
+
+42
+00:02:53,510 --> 00:02:57,530
+لذلك البروتين والبولسوليو بالادس BH فالبروتين
+
+43
+00:02:57,530 --> 00:03:03,330
+هيكون ذائب likewise برضه بنفس الطريقة لما تكون
+
+44
+00:03:03,330 --> 00:03:07,430
+البروتين positive charge وين بيكون positive charge
+
+45
+00:03:07,430 --> 00:03:13,430
+لما تكون الـ BH أقل من الـBI اللي في acidic BHبيكون
+
+46
+00:03:13,430 --> 00:03:17,070
+برضه عندنا شحنات متشابهة بتصير في بينهم repulsion
+
+47
+00:03:17,070 --> 00:03:22,010
+قوة تنافذ فبيكون البروتين والـB سوليو بالاديس this
+
+48
+00:03:22,010 --> 00:03:26,710
+BH التبيئة عند الـ isoelectric point بالظبط اللي
+
+49
+00:03:26,710 --> 00:03:30,150
+هي عند الـ neutral BH هي نقطة الـ isoelectric
+
+50
+00:03:30,150 --> 00:03:33,170
+point بيكون عندنا negative و positive charge
+
+51
+00:03:33,170 --> 00:03:37,610
+negative و positive هيقلل الـ repulsive repulsive
+
+52
+00:03:37,610 --> 00:03:42,320
+أو electrostatic or reduceفماذا سيحصل؟ هو الـ
+
+53
+00:03:42,320 --> 00:03:44,500
+Cause Aggregation and Precipitation
+
+54
+00:03:48,230 --> 00:03:52,130
+مع بعض تتجمع ويصير precipitation أو aggregation
+
+55
+00:03:52,130 --> 00:03:56,150
+للبروتين فإذا أنا بدي أدوب أي بروتين أنا أعطيتك
+
+56
+00:03:56,150 --> 00:03:59,930
+حاجات لك دا و بيلي هذا البروتين بدور على نقطة الـ
+
+57
+00:03:59,930 --> 00:04:02,490
+isoelectric point له بكون عارفة نقطة الـ
+
+58
+00:04:02,490 --> 00:04:06,910
+isoelectric point بجيب مثلا buffer يضبطلي الـ pH
+
+59
+00:04:06,910 --> 00:04:09,690
+عند هذه الـ isoelectric point ويدوب البروتين
+
+60
+00:04:09,690 --> 00:04:14,130
+بيحكيلي الـ isoelectric point لمعظم البروتين بتكون
+
+61
+00:04:14,130 --> 00:04:18,610
+بين pH 4الى 6
+
+62
+00:04:22,760 --> 00:04:27,400
+إذا بدي أعمل أني أرسب أي بروتين أسف على المعلومة
+
+63
+00:04:27,400 --> 00:04:31,200
+اللي جابل إذا بدي أرسب أي بروتين مش أدوب أي بروتين
+
+64
+00:04:31,200 --> 00:04:36,900
+بضبط الـ pH عند نقطة الـ PI إذا بدي أرسبه بضبط الـ pH
+
+65
+00:04:36,900 --> 00:04:40,780
+عند الـ PI أما إذا بدي أدوبه بدي أرفع الـ pH أو
+
+66
+00:04:40,780 --> 00:04:44,420
+أوصلها للـ acid أو بدي أقلل .. بدي أقللها أووصلها
+
+67
+00:04:44,420 --> 00:04:48,370
+للـ acid أو أرفعها أني أوصلها للـ alkalineلكن إذا
+
+68
+00:04:48,370 --> 00:04:53,570
+بدي أرسبه بدي أضبط الـ bh عند الـ bi مش إني أدوبه
+
+69
+00:04:53,570 --> 00:05:03,370
+ماذا هيترسب إذا ضبطت الـ bh عند الـ bi احكيلي
+
+70
+00:05:03,370 --> 00:05:07,810
+أنه solubility of الـ protein ثقابية الـ protein في
+
+71
+00:05:07,810 --> 00:05:10,630
+بعض الـ protein ثقابيتها لا تتأثر بالحرارة يعني
+
+72
+00:05:10,630 --> 00:05:14,870
+حاليا احكيلي بدي أدوبه بالحرارة لايتأثر بالحرارة
+
+73
+00:05:14,870 --> 00:05:19,110
+بسبب تركيبته زي الـ Casein فأنا كيف بدي أزوج وصف
+
+74
+00:05:19,110 --> 00:05:22,270
+الـ ability of casein؟ تحتمل على الـ pH، يعني أوصل
+
+75
+00:05:22,270 --> 00:05:27,910
+الـ pH للـ BI يعني أبط الـ pH توصل للـ isoelectric
+
+76
+00:05:27,910 --> 00:05:31,990
+point لأن حكينا لما متى ما كانت الـ pH بتساوي الـ
+
+77
+00:05:31,990 --> 00:05:36,510
+BI، يترسب الـ protein فإذا أنا بدي أرسبه، بدي ألعب
+
+78
+00:05:36,510 --> 00:05:39,670
+في الـ pH إذا بدي أدوبه، برفع الـ pH، بخليه أعلى من
+
+79
+00:05:39,670 --> 00:05:43,370
+الـ بي اي او اقل من الـ بي اي يكون في الـ acidity او
+
+80
+00:05:43,370 --> 00:05:47,550
+الـ alkalinity هذا بيدوم بس اذا وصلت الـ بي اتش للـ
+
+81
+00:05:47,550 --> 00:05:52,530
+بي اي بدي يتحسن الـ intermediate pH at which
+
+82
+00:05:52,530 --> 00:05:55,370
+protein molecule has charge of 0 نسميها الـ
+
+83
+00:05:55,370 --> 00:05:58,590
+isoelectric point حتى هنا ان الـ isoelectric point
+
+84
+00:05:58,590 --> 00:06:03,130
+هي قيمة بي اتش اللي عندها بتكون ان انت charge 0عند
+
+85
+00:06:03,130 --> 00:06:07,190
+هذه النقطة، يعني نقطة الـ isoelectric point دائمية
+
+86
+00:06:07,190 --> 00:06:11,590
+البروتين تكون أقل ما يمكن، solubility is minimal
+
+87
+00:06:11,590 --> 00:06:17,110
+كيف بدأ أزود الدائمية؟ أني يا إما بأزود الـ pH يا
+
+88
+00:06:17,110 --> 00:06:21,530
+بقلل الـ pH حكينا إن كانت الـ pH أعلى من الـ PI أو
+
+89
+00:06:21,530 --> 00:06:25,030
+أقل من الـ PI، بكون soluble بس متى ما كانت عند الـ
+
+90
+00:06:25,030 --> 00:06:30,180
+PI، بيكون precipitate Precipitation or coagulation
+
+91
+00:06:30,180 --> 00:06:33,500
+of milk protein casein at low pH as milk becomes
+
+92
+00:06:33,500 --> 00:06:37,200
+white بيحكي الفيلم إنها تطبيق على ترسيب البروتين
+
+93
+00:06:37,200 --> 00:06:41,800
+له فساد الحليب، فيه هنا ظاهرة، ظاهرة فساد الحليب
+
+94
+00:06:41,800 --> 00:06:44,860
+كيف فساد الحليب؟ فالجهة الحليب معروفة عنه إن هو
+
+95
+00:06:44,860 --> 00:06:48,820
+وسطه قاعد تمام؟ ومن مكونات الحليب فيه عدة
+
+96
+00:06:48,820 --> 00:06:51,960
+بروتينات، منها البروتين الكازيني وجدوا إن
+
+97
+00:06:51,960 --> 00:06:55,840
+الـisoelectric point للكازين هي حوالي أربعة وسبعة
+
+98
+00:06:57,200 --> 00:07:01,780
+وهي أقل الـ 4.7 أقل من الـ pH بتاعة الحليب فجد
+
+99
+00:07:01,780 --> 00:07:05,960
+الحليب وسط قاعد فبال isoelectric point للكازين اللي
+
+100
+00:07:05,960 --> 00:07:10,660
+موجود في الحليب 4.7 مين أعلى الـ pH قاعد ولا الـ 4.7
+
+101
+00:07:10,660 --> 00:07:14,980
+لأ الـ pH لذن الـ pH للحليب أعلى من الـ PI يعني
+
+102
+00:07:14,980 --> 00:07:18,540
+الشحنة هتكون الـ net charge negative فهيكون الـ
+
+103
+00:07:18,540 --> 00:07:20,000
+protein that kept بتكون في الحليب
+
+104
+00:07:25,940 --> 00:07:29,740
+الكزين اللي موجود في الحليب أربعة وسبعة مينها على
+
+105
+00:07:29,740 --> 00:07:31,760
+البيئة توقعي ولا الاربعة وسبعة
+
+106
+00:07:46,290 --> 00:07:51,150
+سالبة فبتصير طنافر فهيكون سلبة لما يصير فساد
+
+107
+00:07:51,150 --> 00:07:55,190
+للحليب يلمع فيه أورجانيزم يتم تلمع فيه بكتيريا مش
+
+108
+00:07:55,190 --> 00:07:58,970
+هاي الترمال بتحول سكر اللاكتوز الموجود في الحليب لـ
+
+109
+00:07:58,970 --> 00:08:04,210
+Lactic Acid و Lactic Acid هذا حمض لأن هو وسط قاعد
+
+110
+00:08:04,210 --> 00:08:07,230
+الحليب مع الـ بكتيريا اللي نمت فيه هو القوانات
+
+111
+00:08:07,230 --> 00:08:11,730
+للاكتك أسد Lactic Acid هذا هيقلل البيئات اللي هو
+
+112
+00:08:11,730 --> 00:08:14,650
+اللي بيقللها بيقللها وصولا إلى الـ pH
+
+113
+00:08:18,250 --> 00:08:22,230
+مجد خسارة الحليب هو الـ Lactic Acid وهو ربعاً حمض
+
+114
+00:08:22,230 --> 00:08:27,970
+والحليب أصلا وسط قاعد فجد قاعدة محمض بيقلل الـ
+
+115
+00:08:27,970 --> 00:08:34,550
+pH من قاعدة الحليب وصولا إلى درجة الحموظة فبتقترب
+
+116
+00:08:34,550 --> 00:08:40,470
+من الـBi للكازين اللي هي 4 و7 مدام الـBi وصلت
+
+117
+00:08:40,470 --> 00:08:45,070
+الـBH صاروا متساويين إذا بيترسب الكازين فبيصير فيه
+
+118
+00:08:45,070 --> 00:08:46,590
+تنسيق للكازين
+
+119
+00:09:13,180 --> 00:09:17,340
+فكيف بتعرف الـ isoelectric point للكازين؟ بناء على
+
+120
+00:09:17,340 --> 00:09:20,960
+الـ principle اللي هو المبدأ إنه بدي أستخدم أسيتات
+
+121
+00:09:20,960 --> 00:09:26,720
+buffer بستخدم buffer اللي قيم BH مختلفة عشان أكشف
+
+122
+00:09:26,720 --> 00:09:29,740
+عن الـ isoelectric point of كازين يعني أنا بدي
+
+123
+00:09:29,740 --> 00:09:33,740
+ألعب بالـ BH بغير الـ BH حكيمة مدى ما بدي أعرف الـ
+
+124
+00:09:33,740 --> 00:09:37,840
+isoelectric point بعرف بغير الـ BH مثلا بستخدم
+
+125
+00:09:37,840 --> 00:09:42,280
+تسعة نبيب و أحط عليهم روتين الكازين وكل نبوبة فيها
+
+126
+00:09:54,070 --> 00:09:58,670
+الأول تانية تلاتة و تمانية بعدها أربعة و واحد أربع
+
+127
+00:09:58,670 --> 00:10:03,350
+واربع أربع وسبعة خمسة خمسة و تلاتة خمسة و ستة خمسة
+
+128
+00:10:03,350 --> 00:10:07,470
+و جسد عنده قيم بيقات مختلفة تمام أنا استخدمت هذه
+
+129
+00:10:07,470 --> 00:10:13,050
+القنادير و حطيت فيهم كمية من الحمب بتركيز مختلفة
+
+130
+00:10:29,150 --> 00:10:33,990
+معادلة بحسم من خلالها دياتي أنا عندي عدة قنابيل
+
+131
+00:10:33,990 --> 00:10:38,590
+تحتوي على protein الكازين ومحطوط معاها buffer الـ
+
+132
+00:10:38,590 --> 00:10:43,470
+buffer هذه تعمل من حمض وقاعدة تركيز الحمض مختلفة
+
+133
+00:10:43,470 --> 00:10:47,410
+أنا عاملانا تخفيف كل أنبوب عن التعنى ليش؟ عشان
+
+134
+00:10:47,410 --> 00:10:51,470
+أغير البيئات بعدين بنسك هذه القنابيل باخد الأنبوب
+
+135
+00:10:51,470 --> 00:10:51,950
+الأول
+
+136
+00:11:10,940 --> 00:11:14,340
+الأنبوبة المترسّب فيها البروتين الكازيين من هذه
+
+137
+00:11:14,340 --> 00:11:17,180
+الأنبوبة هي قيمة الـ pH
+
+138
+00:11:42,230 --> 00:11:47,330
+الـ BKA للي 
+
+139
+00:11:47,330 --> 00:11:55,310
+احنا مستخدمينها هي معروفة 4.75 BKA 4.75 الـ base
+
+140
+00:11:55,310 --> 00:11:58,290
+القاعد اللي أنا استخدمتها اللي بستخدمها في التجربة
+
+141
+00:11:58,290 --> 00:12:02,690
+هي كازين أسيتات صوديوم مش مفهوم منكوا تعرفوا
+
+142
+00:12:11,800 --> 00:12:15,740
+الكزين أسيتات Sodium الـ Milk Base تركيزها 1 من 10
+
+143
+00:12:15,740 --> 00:12:19,460
+مين اللي غير تركيزه الـ Acetic Acid فكل أنبوب
+
+144
+00:12:19,460 --> 00:12:25,200
+هيكون له تركيز لبحن مختلف عن التاني عشان يتغير قيم
+
+145
+00:12:25,200 --> 00:12:30,220
+البيئات معروفة أن هذه التركيز موجودة في آخر في قبل
+
+146
+00:12:30,220 --> 00:12:33,220
+الأخيرة سواء في قبل الأخيرة في جدول موجودة
+
+147
+00:12:35,490 --> 00:12:39,350
+جداش تركيزه في النبوب الأول مثلا 1 و 6، للنبوب
+
+148
+00:12:39,350 --> 00:12:43,090
+التالت 8 من 10، للنبوب التالت 4 من 10 و هكذا، هذه
+
+149
+00:12:43,090 --> 00:12:47,030
+محسوبة اللي هي تركيز الحمض، بحطها في المعادلة، صار
+
+150
+00:12:47,030 --> 00:12:51,430
+عندي معروف تركيز الـBKA جداش، والبس 1 من 10،
+
+151
+00:12:51,430 --> 00:12:54,590
+والacetic acid بتاخدها من الجدول، واحسب الـBH
+
+152
+00:12:54,590 --> 00:12:58,670
+للنبوب الأول، BH للنبوب التاني، للتالت، فهذا
+
+153
+00:12:58,670 --> 00:13:03,660
+الجدول اللي في الأخرهو تركيز الحمض في الجداش في كل
+
+154
+00:13:03,660 --> 00:13:10,020
+انبوبة والـ pH جداش موجودة لكل محسوبة لكل انبوبة
+
+155
+00:13:10,020 --> 00:13:12,780
+كيف حسبها من خلال المعادلة اللي فوق اللي طبعا
+
+156
+00:13:12,780 --> 00:13:18,100
+حكينا عنها
+
+157
+00:13:18,100 --> 00:13:22,440
+أكررنا إن حكينا إن البروتينات عشان اخليها تترسب
+
+158
+00:13:22,440 --> 00:13:26,460
+لازم أوصيها للـ isoelectric point to aggregate and
+
+159
+00:13:26,460 --> 00:13:27,120
+dissipate
+
+160
+00:13:32,930 --> 00:13:35,510
+احنا مختلفة تتجادل مع بعضها
+
+161
+00:13:47,940 --> 00:13:51,160
+حسب مين؟ حسب الـ Amino Acid Sequence حسب عدد الـ
+
+162
+00:13:51,160 --> 00:13:55,060
+Anionic والكاتيونيك الـ Growth فأنا بحكي لي هدفات
+
+163
+00:13:55,060 --> 00:14:00,100
+بستخدمها و بستفيد منها لو بدي أفصل البروتين من عدة
+
+164
+00:14:00,100 --> 00:14:03,320
+بروتينات يعني مثلا عندي حليب يحتوي على عدة
+
+165
+00:14:03,320 --> 00:14:07,420
+بروتينات من ضمنها البروتين الكازين كيف بدي أخد من
+
+166
+00:14:07,420 --> 00:14:11,200
+الحليب بس البروتين الكازين و أسيب الباقي؟ بعرف
+
+167
+00:14:11,200 --> 00:14:15,220
+الـBI للكازين و بوصلها الـBH للـBI
+
+168
+00:14:35,740 --> 00:14:42,140
+ساوى للـ VI هالوراسة في تجربتنا لليوم مش البدوات
+
+169
+00:14:42,140 --> 00:14:45,560
+اللي بدي استخدمها بستخدم acetic acid اللي هو الحمض
+
+170
+00:14:45,560 --> 00:14:48,480
+بستخدم البروتين الكازين اللي هو اللي أنا بدورله
+
+171
+00:14:48,480 --> 00:14:50,980
+على الـ isoelectric point اللي بتعرفه isoelectric
+
+172
+00:14:50,980 --> 00:14:54,760
+point بستخدم NaOH عشان ارفع البيئات عشان
+
+173
+00:14:54,760 --> 00:14:58,260
+اوصلها على القاعدة الحمض بدي نزل البيئات NaOH
+
+174
+00:14:58,260 --> 00:14:58,920
+بيرفع
+
+175
+0
+
+201
+00:17:11,550 --> 00:17:15,370
+من الحمض من الحمض من الـ acetic acid لأن نبيب
+
+202
+00:17:15,370 --> 00:17:18,490
+التاني ماليش يحط عليها من الحمض كيف بدي اعمل بتاخد
+
+203
+00:17:18,490 --> 00:17:22,940
+من أنبوب واحد خمسة مل على أنبوب اثنين أنبوب واحد
+
+204
+00:17:22,940 --> 00:17:26,320
+صار فيه ستة وثمانية ديستل واتر وثلاثة واثنين
+
+205
+00:17:26,320 --> 00:17:30,340
+راسيتي كهسان أما باقي الأنابيب فيها خمسة مل من
+
+206
+00:17:30,340 --> 00:17:34,120
+ديستل واتر بدي اجي على أنبوب واحد اللي فيه ديستل 
+
+207
+00:17:34,120 --> 00:17:41,460
+واتر محاني بدي اعمل تخفيفات للحمض فبدي اخد عامل mix
+
+208
+00:17:41,460 --> 00:17:48,120
+واخد خمسة مل من أنبوب ثلاثة
+
+209
+00:17:48,120 --> 00:17:52,800
+لاربعة وعامل mix واخد خمسة مل من أنبوب أربعة لخمسة 
+
+210
+00:17:52,800 --> 00:17:58,370
+وخمسة مل من خمسة لستة واخد خمسة مل من ستة لسبعة و
+
+211
+00:17:58,370 --> 00:18:01,990
+بعدين خمسة مل من سبعة لثمانية وبعدين خمسة مل من
+
+212
+00:18:01,990 --> 00:18:06,290
+ثمانية لتسعة صارت كل الأنابيب تحتوي على خمسة مل 
+
+213
+00:18:06,290 --> 00:18:10,870
+ديستل واطر من رقم اثنين لتسعة تحتوي خمسة مل ديستل
+
+214
+00:18:10,870 --> 00:18:16,150
+واطر وخمسة مل من الأنبوب اللي جبنا عملتلهم mix
+
+215
+00:18:16,150 --> 00:18:19,830
+مع بعض و contango الخمسة فظل المحصلة بس خمسة مل 
+
+216
+00:18:19,830 --> 00:18:24,850
+ديستل واطر مع حمض مع بعض هل جيت معادة الأنبوب
+
+217
+00:18:24,850 --> 00:18:29,260
+الأخيرة؟ من الأنبوب الأخيرة صار فيها خمسة مل زيادة
+
+218
+00:18:29,260 --> 00:18:32,680
+لإن أنا ما نجلتش لحد بعدها فبدي أطلع منها خمسة مل 
+
+219
+00:18:32,680 --> 00:18:36,540
+وأكرها، خلاص بتتخلص منها، أجيت أنا صرت عاملة
+
+220
+00:18:36,540 --> 00:18:40,700
+تخفيفات تدريجية Serial Dilution للأنابيب و للحمض
+
+221
+00:18:40,700 --> 00:18:44,500
+اللي أنا أضفته للـacetic acid أنا بس على الأول أضفت
+
+222
+00:18:44,500 --> 00:18:48,680
+استيك أسد وبعدين بلشت أنقل من الأنبوب 1 لـ 2، 2 لـ 3،
+
+223
+00:18:48,680 --> 00:18:53,060
+3 لـ 4، وهكذا وصلت للآخر الـ TUE فعملت Serial 
+
+224
+00:18:53,060 --> 00:18:56,980
+Dilution، تخفيف تدريجي للحمض ليش هدا التخفيف؟ عشان
+
+225
+00:18:56,980 --> 00:19:01,400
+أغير قيم الـ pH عشان أعرف وين الـ pH متساوي للـ 
+
+226
+00:19:01,400 --> 00:19:07,300
+isoelectric point بعد هيك باجي بتحط في كل TUE بات
+
+227
+00:19:07,300 --> 00:19:10,880
+من 1 لـ 9 من الـ buffer اللي أنا حضرتة في الـ flask
+
+228
+00:19:14,160 --> 00:19:19,180
+بعدين مل حضرت بفر حكاية بتاخد منه واحد مل على تيوب
+
+229
+00:19:19,180 --> 00:19:22,720
+واحد وبعدين من الـ flask نفسه واحد مل على تيوب 
+
+230
+00:19:22,720 --> 00:19:26,260
+اثنين وبعدين من الـ flask نفسه واحد مل على تيوب
+
+231
+00:19:26,260 --> 00:19:30,900
+ثلاثة وهكذا لآخر تيوب بتقدر نضيف واحد مل من الـ 
+
+232
+00:19:30,900 --> 00:19:36,920
+flask على كل تيوبها وبتركه لمدة نص ساعة بترك كل
+
+233
+00:19:36,920 --> 00:19:41,200
+القنابيب لمدة نص ساعة بعد نص ساعة بجلد القنابيب 
+
+234
+00:19:41,200 --> 00:19:45,150
+بشوف همسك تيوب تيوب تيوب واحدة همسكها، شوف فيها
+
+235
+00:19:45,150 --> 00:19:48,590
+precipitate تحت ولا مافيش فيها إذا ترسّب، يبقى الـ 
+
+236
+00:19:48,590 --> 00:19:52,890
+protein هنا مترسّب عند قيمة الـ pH هذه إذا هنا هو
+
+237
+00:19:52,890 --> 00:19:56,570
+بترسّب، إذا هذه هي الـ PI إذا مش مترسّب، بدي أنتج
+
+238
+00:19:56,570 --> 00:20:00,090
+إلى اللي بعدها، بشوف كل الأنابيب، أي أنبوب مترسّب
+
+239
+00:20:00,090 --> 00:20:04,410
+عندها، إذا عند هذه الـ pH متساوي الـ PI
+
+240
+00:20:13,210 --> 00:20:17,230
+أما بيحكي إن هم لما اشتغلوا التجربة وجدوا أن الـ
+
+241
+00:20:17,230 --> 00:20:24,010
+isoelectric point للكازين هي 4 و7 
+
+242
+00:20:24,010 --> 00:20:29,390
+يعني المفروض تطلع معناه الـ precipitate
+
+243
+00:20:29,390 --> 00:20:34,350
+للكازين هي 4 و7 لأن الـ PI للكازين هي 4 و
+
+244
+00:20:34,350 --> 00:20:39,410
+7 ففي الجدول أي tube عندها الـ pH 4 و7 هي tube
+
+245
+00:20:39,410 --> 00:20:45,680
+رقم 5 هو المعنى فيها أعلى precipitates كيف احنا
+
+246
+00:20:45,680 --> 00:20:50,660
+هنسجل النتيجة نحكي الـ result الـ PI حكي انها 4.75
+
+247
+00:20:50,660 --> 00:20:54,340
+النتيجة هسجلها همسك تيوب واحد يفترض ان مايكونش 
+
+248
+00:20:54,340 --> 00:20:57,440
+فيها precipitates و اكتب عليها negative اثنين
+
+249
+00:20:57,440 --> 00:21:02,280
+مافيش فيها negative ثلاثة negative اربعة قريبة من
+
+250
+00:21:02,280 --> 00:21:06,940
+الـ PI للكازين من الـ pH من أربعة وسبعة لكن مش هي 
+
+251
+00:21:06,940 --> 00:21:08,860
+فهتكون ممكن تكون plus واحد
+
+252
+00:21:12,560 --> 00:21:15,720
+الـ Precision أعلى لـ Preceptive maximum decision
+
+253
+00:21:15,720 --> 00:21:19,420
+plus 3 إذا كان الـ Preceptive بسيط plus 1 إذا
+
+254
+00:21:19,420 --> 00:21:25,400
+وصلت plus 2 هو أسجل النتيجة المفروض ان حياتيه برقم
+
+255
+00:21:25,400 --> 00:21:29,060
+5 هي اللي تطلع عندنا أعلى precipitate إذا طلع 
+
+256
+00:21:29,060 --> 00:21:32,920
+عندنا خطأ بنحسب نسبة الخطأ الـ Error اللي هي
+
+257
+00:21:32,920 --> 00:21:37,020
+الـ Experimental Value ناقص الـ Theoretical Value 
+
+258
+00:21:37,020 --> 00:21:40,500
+على الـ Theoretical Value ضارب 100 اللي هي القيمة
+
+259
+00:21:40,500 --> 00:21:43,220
+المحسوبة ناقص القيمة العملية اللي أنا اشتغلتها على 
+
+260
+00:21:43,220 --> 00:21:45,400
+القيمة المحسوبة ضارب 100 
diff --git a/PL9fwy3NUQKwZ1Q7zOg1ObgufPMjRI3WY1/zJp2OlvRd8A_raw.json b/PL9fwy3NUQKwZ1Q7zOg1ObgufPMjRI3WY1/zJp2OlvRd8A_raw.json
new file mode 100644
index 0000000000000000000000000000000000000000..7811654dc01ecaea7ec9001680dc6a40ca443b06
--- /dev/null
+++ b/PL9fwy3NUQKwZ1Q7zOg1ObgufPMjRI3WY1/zJp2OlvRd8A_raw.json
@@ -0,0 +1 @@
+{"segments": [{"id": 1, "seek": 1694, "start": 3.62, "end": 16.94, "text": "أو وين بيدوب وين بيكون soluble وين بيكون precipitate يذا فاكرين لما حكينا عن ال ionization عن ال amino acid كيف بيتصرف في الأوساط المختلفة لما حاطنا فيه alkaline و فيه neutral و فيه acidic pH", "tokens": [10721, 2407, 4032, 9957, 4724, 25708, 37746, 4032, 9957, 4724, 1829, 30544, 1404, 836, 306, 4032, 9957, 4724, 1829, 30544, 23354, 8086, 7251, 15730, 6156, 995, 37983, 9957, 5296, 15042, 11331, 4117, 1829, 8315, 18871, 2423, 17437, 2144, 18871, 2423, 24674, 8258, 9122, 33911, 4724, 36081, 9381, 28480, 8978, 16247, 41779, 41193, 9673, 46456, 46538, 3660, 5296, 15042, 11331, 41193, 8315, 8978, 3224, 44220, 533, 4032, 8978, 3224, 10598, 4032, 8978, 3224, 39514, 21677], "avg_logprob": -0.23770832856496174, "compression_ratio": 1.5944444444444446, "no_speech_prob": 4.571676254272461e-05, "words": [{"start": 3.62, "end": 4.28, "word": "أو", "probability": 0.544830322265625}, {"start": 4.28, "end": 4.46, "word": " وين", "probability": 0.6943359375}, {"start": 4.46, "end": 5.0, "word": " بيدوب", "probability": 0.7335611979166666}, {"start": 5.0, "end": 5.6, "word": " وين", "probability": 0.8720703125}, {"start": 5.6, "end": 5.88, "word": " بيكون", "probability": 0.9462890625}, {"start": 5.88, "end": 6.24, "word": " soluble", "probability": 0.6434122721354166}, {"start": 6.24, "end": 6.4, "word": " وين", "probability": 0.97998046875}, {"start": 6.4, "end": 6.68, "word": " بيكون", "probability": 0.96826171875}, {"start": 6.68, "end": 7.5, "word": " precipitate", "probability": 0.889404296875}, {"start": 7.5, "end": 8.08, "word": " يذا", "probability": 0.171844482421875}, {"start": 8.08, "end": 8.46, "word": " فاكرين", "probability": 0.9244384765625}, {"start": 8.46, "end": 8.64, "word": " لما", "probability": 0.87060546875}, {"start": 8.64, "end": 8.96, "word": " حكينا", "probability": 0.96435546875}, {"start": 8.96, "end": 9.1, "word": " عن", "probability": 0.9716796875}, {"start": 9.1, "end": 9.2, "word": " ال", "probability": 0.9521484375}, {"start": 9.2, "end": 9.9, "word": " ionization", "probability": 0.818603515625}, {"start": 9.9, "end": 10.1, "word": " عن", "probability": 0.8837890625}, {"start": 10.1, "end": 10.22, "word": " ال", "probability": 0.92724609375}, {"start": 10.22, "end": 10.5, "word": " amino", "probability": 0.74755859375}, {"start": 10.5, "end": 10.88, "word": " acid", "probability": 0.89501953125}, {"start": 10.88, "end": 11.2, "word": " كيف", "probability": 0.967041015625}, {"start": 11.2, "end": 11.76, "word": " بيتصرف", "probability": 0.935546875}, {"start": 11.76, "end": 11.88, "word": " في", "probability": 0.8701171875}, {"start": 11.88, "end": 12.3, "word": " الأوساط", "probability": 0.96826171875}, {"start": 12.3, "end": 13.06, "word": " المختلفة", "probability": 0.994140625}, {"start": 13.06, "end": 13.52, "word": " لما", "probability": 0.72314453125}, {"start": 13.52, "end": 13.86, "word": " حاطنا", "probability": 0.857421875}, {"start": 13.86, "end": 14.18, "word": " فيه", "probability": 0.944580078125}, {"start": 14.18, "end": 14.8, "word": " alkaline", "probability": 0.8271484375}, {"start": 14.8, "end": 14.96, "word": " و", "probability": 0.638671875}, {"start": 14.96, "end": 15.1, "word": " فيه", "probability": 0.645263671875}, {"start": 15.1, "end": 15.58, "word": " neutral", "probability": 0.97802734375}, {"start": 15.58, "end": 15.86, "word": " و", "probability": 0.9833984375}, {"start": 15.86, "end": 16.1, "word": " فيه", "probability": 0.69921875}, {"start": 16.1, "end": 16.48, "word": " acidic", "probability": 0.97021484375}, {"start": 16.48, "end": 16.94, "word": " pH", "probability": 0.82763671875}], "temperature": 1.0}, {"id": 2, "seek": 4152, "start": 17.48, "end": 41.52, "text": "نفسها للبروتين، لأن البروتين هو عبارة عن سلسلة من الأحماض الأمينية أنا لو جبت بروتين وحطيته في الـ neutral pH الـ neutral pH حكينا شو بتكون ال net charge في الأمين واسد، بتكون Zero ال net charge Zero يعني عدد الشحنات الموجبة 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"end": 84.19, "text": "فإذا الوين هيترسّب البروتين عند الـ neutral pH اللي هي بتكون فيها الـ pH عند الـ isoelectric point بتساوي الـ isoelectric point فنقطة الـ isoelectric point هيترسّب البروتين لكن مثلا لو حطته في acidic media يعني الـ pH كانت أقل من الـ isoelectric point", "tokens": [5172, 28814, 15730, 2423, 2407, 9957, 39896, 2655, 2288, 3794, 11703, 3555, 2423, 26890, 35473, 9957, 43242, 2423, 39184, 10598, 21677, 13672, 1829, 39896, 39894, 30544, 8978, 11296, 2423, 39184, 21677, 43242, 2423, 39184, 307, 7921, 38421, 935, 39894, 3794, 995, 45865, 2423, 39184, 307, 7921, 38421, 935, 6156, 1863, 47432, 3660, 2423, 39184, 307, 7921, 38421, 935, 39896, 2655, 2288, 3794, 11703, 3555, 2423, 26890, 35473, 9957, 44381, 50113, 15040, 45164, 11331, 9566, 47395, 8978, 39514, 3021, 37495, 22653, 2423, 39184, 21677, 25961, 2655, 5551, 4587, 1211, 9154, 2423, 39184, 307, 7921, 38421, 935], "avg_logprob": -0.15136718517169356, "compression_ratio": 1.9123711340206186, "no_speech_prob": 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220.88, "end": 224.5, "text": "فماذا سيحصل؟ هو الـ Cause Aggregation and Precipitation", "tokens": [5172, 2304, 45636, 995, 8608, 1829, 5016, 36520, 22807, 31439, 2423, 39184, 7544, 438, 41512, 20167, 293, 430, 3326, 79, 4614], "avg_logprob": -0.6704545481638475, "compression_ratio": 0.8987341772151899, "no_speech_prob": 3.4570693969726562e-06, "words": [{"start": 220.88, "end": 221.34, "word": "فماذا", "probability": 0.5283660888671875}, {"start": 221.34, "end": 222.02, "word": " سيحصل؟", "probability": 0.62802734375}, {"start": 222.02, "end": 222.18, "word": " هو", "probability": 0.1719970703125}, {"start": 222.18, "end": 222.32, "word": " الـ", "probability": 0.56207275390625}, {"start": 222.32, "end": 222.62, "word": " Cause", "probability": 0.398956298828125}, {"start": 222.62, "end": 223.34, "word": " Aggregation", "probability": 0.867919921875}, {"start": 223.34, "end": 223.74, "word": " and", "probability": 0.77001953125}, {"start": 223.74, "end": 224.5, "word": " 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point للكازين هي 4 و 7 يعني المفروض تطلع معناه الـ «به» الـ precipitate للكازين هي «به» 4 و 7 لأن الـ «بي» للكازين هي 4 و 7 ففي الجدول أي tube عندها الـ «به» 4 و 7 هي tube رقم 5", "tokens": [10721, 15042, 4724, 1829, 5016, 4117, 1829, 36145, 8032, 2304, 5296, 15042, 1975, 8592, 2655, 17082, 1211, 14407, 16712, 7435, 25513, 3660, 49610, 3215, 14407, 14739, 2423, 39184, 4657, 21292, 5933, 2423, 39184, 4657, 21292, 5933, 2423, 39184, 307, 7921, 38421, 935, 24976, 4117, 31377, 9957, 39896, 1017, 4032, 1614, 37495, 22653, 9673, 5172, 32887, 11242, 6055, 9566, 1211, 3615, 20449, 8315, 3224, 2423, 39184, 4657, 3555, 3224, 5933, 2423, 39184, 23354, 8086, 24976, 4117, 31377, 9957, 39896, 4657, 3555, 3224, 5933, 1017, 4032, 1614, 5296, 33456, 2423, 39184, 4657, 21292, 5933, 24976, 4117, 31377, 9957, 39896, 1017, 4032, 1614, 6156, 41185, 25724, 3215, 12610, 36632, 9917, 43242, 11296, 2423, 39184, 4657, 3555, 3224, 5933, 1017, 4032, 1614, 39896, 9917, 12602, 4587, 2304, 1025], "avg_logprob": 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"word": " المفروض", "probability": 0.9884033203125}, {"start": 1225.41, "end": 1225.79, "word": " تطلع", "probability": 0.9820556640625}, {"start": 1225.79, "end": 1226.35, "word": " معناه", "probability": 0.6656901041666666}, {"start": 1226.35, "end": 1226.59, "word": " الـ", "probability": 0.733154296875}, {"start": 1226.59, "end": 1227.43, "word": " «به»", "probability": 0.58184814453125}, {"start": 1227.43, "end": 1227.99, "word": " الـ", "probability": 0.7080078125}, {"start": 1227.99, "end": 1229.39, "word": " precipitate", "probability": 0.859619140625}, {"start": 1229.39, "end": 1229.97, "word": " للكازين", "probability": 0.8876953125}, {"start": 1229.97, "end": 1230.09, "word": " هي", "probability": 0.50537109375}, {"start": 1230.09, "end": 1230.91, "word": " «به»", "probability": 0.771484375}, {"start": 1230.91, "end": 1231.17, "word": " 4", "probability": 0.7138671875}, {"start": 1231.17, "end": 1231.43, "word": " و", "probability": 0.9775390625}, {"start": 1231.43, "end": 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"probability": 0.4580078125}, {"start": 1236.61, "end": 1237.21, "word": " عندها", "probability": 0.990478515625}, {"start": 1237.21, "end": 1237.59, "word": " الـ", "probability": 0.759521484375}, {"start": 1237.59, "end": 1237.97, "word": " «به»", "probability": 0.9056396484375}, {"start": 1237.97, "end": 1238.23, "word": " 4", "probability": 0.9716796875}, {"start": 1238.23, "end": 1238.49, "word": " و", "probability": 0.9931640625}, {"start": 1238.49, "end": 1238.81, "word": " 7", "probability": 0.9384765625}, {"start": 1238.81, "end": 1239.13, "word": " هي", "probability": 0.95703125}, {"start": 1239.13, "end": 1239.41, "word": " tube", "probability": 0.82470703125}, {"start": 1239.41, "end": 1239.73, "word": " رقم", "probability": 0.9900716145833334}, {"start": 1239.73, "end": 1239.83, "word": " 5", "probability": 0.63818359375}], "temperature": 1.0}, {"id": 49, "seek": 126885, "start": 1242.22, "end": 1268.86, "text": "هو المعنى فيها أعلى precipitates كيف احنا هنسجل النتيجة نحكي ال result ال BKA حكي انها 4.75 النتيجة هسجلها همسك تيوب واحد يفترض ان مايكونش فيها precipitates و اكتب عليها negative اتنين مافيش فيها negative تلاتة negative اربع قريبة من ال BH للكزي من ال BY من اربعة وسبعة لكن مش هي فهتكون ممكن تكون plus واحد", "tokens": [3224, 2407, 9673, 3615, 1863, 7578, 8978, 11296, 5551, 3615, 23942, 23354, 30035, 9122, 33911, 1975, 5016, 8315, 8032, 1863, 3794, 7435, 1211, 28239, 31371, 7435, 3660, 8717, 5016, 4117, 1829, 2423, 1874, 2423, 363, 16135, 11331, 4117, 1829, 16472, 11296, 1017, 13, 11901, 28239, 31371, 7435, 3660, 8032, 3794, 7435, 1211, 11296, 8032, 2304, 3794, 4117, 6055, 1829, 37746, 36764, 24401, 7251, 5172, 2655, 43042, 16472, 19446, 1829, 30544, 8592, 8978, 11296, 23354, 30035, 4032, 1975, 4117, 2655, 3555, 25894, 11296, 3671, 1975, 2655, 1863, 9957, 19446, 41185, 8592, 8978, 11296, 3671, 6055, 1211, 9307, 3660, 3671, 1975, 25513, 3615, 12174, 16572, 49401, 9154, 2423, 40342, 24976, 4117, 11622, 1829, 9154, 2423, 26930, 9154, 1975, 25513, 27884, 4032, 35457, 27884, 44381, 37893, 39896, 6156, 3224, 2655, 30544, 3714, 43020, 6055, 30544, 1804, 36764, 24401], "avg_logprob": -0.22782628150547252, "compression_ratio": 1.8239700374531835, "no_speech_prob": 2.1457672119140625e-06, "words": [{"start": 1242.22, "end": 1242.44, "word": "هو", "probability": 0.5157470703125}, {"start": 1242.44, "end": 1242.98, "word": " المعنى", "probability": 0.962158203125}, {"start": 1242.98, "end": 1243.36, "word": " فيها", "probability": 0.923828125}, {"start": 1243.36, "end": 1243.86, "word": " أعلى", "probability": 0.9423828125}, {"start": 1243.86, "end": 1244.68, "word": " precipitates", "probability": 0.666015625}, {"start": 1244.68, "end": 1245.48, "word": " كيف", "probability": 0.97021484375}, {"start": 1245.48, "end": 1245.68, "word": " احنا", "probability": 0.762939453125}, {"start": 1245.68, "end": 1246.22, "word": " هنسجل", "probability": 0.96171875}, {"start": 1246.22, "end": 1246.96, "word": " النتيجة", "probability": 0.968017578125}, {"start": 1246.96, "end": 1247.78, "word": " نحكي", "probability": 0.8739013671875}, {"start": 1247.78, "end": 1247.88, "word": " ال", "probability": 0.27685546875}, {"start": 1247.88, "end": 1248.22, "word": " result", "probability": 0.8173828125}, {"start": 1248.22, "end": 1248.42, "word": " ال", "probability": 0.5947265625}, {"start": 1248.42, "end": 1248.82, "word": " BKA", "probability": 0.475341796875}, {"start": 1248.82, "end": 1249.16, "word": " حكي", "probability": 0.8723958333333334}, {"start": 1249.16, "end": 1249.42, "word": " انها", "probability": 0.6175537109375}, {"start": 1249.42, "end": 1249.72, "word": " 4", "probability": 0.93994140625}, {"start": 1249.72, "end": 1250.66, "word": ".75", "probability": 0.926025390625}, {"start": 1250.66, "end": 1251.74, "word": " النتيجة", "probability": 0.8514404296875}, {"start": 1251.74, "end": 1252.4, "word": " هسجلها", "probability": 0.98681640625}, {"start": 1252.4, "end": 1252.76, "word": " همسك", "probability": 0.947998046875}, {"start": 1252.76, "end": 1253.04, "word": " تيوب", "probability": 0.4936116536458333}, {"start": 1253.04, "end": 1253.38, "word": " واحد", "probability": 0.892578125}, {"start": 1253.38, "end": 1253.78, "word": " يفترض", "probability": 0.9176025390625}, {"start": 1253.78, "end": 1253.92, "word": " ان", "probability": 0.6279296875}, {"start": 1253.92, "end": 1254.34, "word": " مايكونش", "probability": 0.64599609375}, {"start": 1254.34, "end": 1254.58, "word": " فيها", "probability": 0.96337890625}, {"start": 1254.58, "end": 1255.16, "word": " precipitates", "probability": 0.9296875}, {"start": 1255.16, "end": 1255.32, "word": " و", "probability": 0.77880859375}, {"start": 1255.32, "end": 1255.54, "word": " اكتب", "probability": 0.90576171875}, {"start": 1255.54, "end": 1255.8, "word": " عليها", "probability": 0.974853515625}, {"start": 1255.8, "end": 1256.24, "word": " negative", "probability": 0.791015625}, {"start": 1256.24, "end": 1257.44, "word": " اتنين", "probability": 0.8145751953125}, {"start": 1257.44, "end": 1257.8, "word": " مافيش", "probability": 0.695556640625}, {"start": 1257.8, "end": 1258.1, "word": " فيها", "probability": 0.986083984375}, {"start": 1258.1, "end": 1258.68, "word": " negative", "probability": 0.91650390625}, {"start": 1258.68, "end": 1260.44, "word": " تلاتة", "probability": 0.943603515625}, {"start": 1260.44, "end": 1261.0, "word": " negative", "probability": 0.9404296875}, {"start": 1261.0, "end": 1261.56, "word": " اربع", "probability": 0.7587890625}, {"start": 1261.56, "end": 1262.1, "word": " قريبة", "probability": 0.95947265625}, {"start": 1262.1, "end": 1262.28, "word": " من", "probability": 0.99072265625}, {"start": 1262.28, "end": 1262.44, "word": " ال", "probability": 0.9033203125}, {"start": 1262.44, "end": 1262.72, "word": " BH", "probability": 0.36474609375}, {"start": 1262.72, "end": 1263.98, "word": " للكزي", "probability": 0.61932373046875}, {"start": 1263.98, "end": 1264.16, "word": " من", "probability": 0.94287109375}, {"start": 1264.16, "end": 1264.34, "word": " ال", "probability": 0.90576171875}, {"start": 1264.34, "end": 1264.5, "word": " BY", "probability": 0.357666015625}, {"start": 1264.5, "end": 1264.82, "word": " من", "probability": 0.98779296875}, {"start": 1264.82, "end": 1265.1, "word": " اربعة", "probability": 0.653076171875}, {"start": 1265.1, "end": 1265.62, "word": " وسبعة", "probability": 0.8211263020833334}, {"start": 1265.62, "end": 1266.46, "word": " لكن", "probability": 0.9423828125}, {"start": 1266.46, "end": 1266.74, "word": " مش", "probability": 0.96533203125}, {"start": 1266.74, "end": 1266.94, "word": " هي", "probability": 0.73974609375}, {"start": 1266.94, "end": 1267.4, "word": " فهتكون", "probability": 0.883544921875}, {"start": 1267.4, "end": 1267.68, "word": " ممكن", "probability": 0.9794921875}, {"start": 1267.68, "end": 1268.02, "word": " تكون", "probability": 0.9921875}, {"start": 1268.02, "end": 1268.36, "word": " plus", "probability": 0.7724609375}, {"start": 1268.36, "end": 1268.86, "word": " واحد", "probability": 0.96484375}], "temperature": 1.0}, {"id": 50, "seek": 128070, "start": 1272.56, "end": 1280.7, "text": "الـ Precision أعلى لـ Preceptive maximum decision plus 3 إذا كان الـ Preceptive بسيط plus 1 إذا واصلت plus 2", "tokens": [6027, 39184, 6001, 40832, 5551, 3615, 23942, 5296, 39184, 6001, 1336, 488, 6674, 3537, 1804, 805, 11933, 15730, 25961, 2423, 39184, 6001, 1336, 488, 4724, 3794, 1829, 9566, 1804, 502, 11933, 15730, 4032, 33546, 1211, 2655, 1804, 568], "avg_logprob": -0.44591347376505536, "compression_ratio": 1.2897196261682242, "no_speech_prob": 0.0, "words": [{"start": 1272.56, "end": 1272.66, "word": "الـ", "probability": 0.18902587890625}, {"start": 1272.66, "end": 1272.92, "word": " Precision", "probability": 0.54534912109375}, {"start": 1272.92, "end": 1273.52, "word": " أعلى", "probability": 0.7139485677083334}, {"start": 1273.52, "end": 1273.72, "word": " لـ", "probability": 0.67431640625}, {"start": 1273.72, "end": 1274.5, "word": " Preceptive", "probability": 0.4537760416666667}, {"start": 1274.5, "end": 1275.2, "word": " maximum", "probability": 0.35009765625}, {"start": 1275.2, "end": 1275.72, "word": " decision", "probability": 0.462158203125}, {"start": 1275.72, "end": 1275.96, "word": " plus", "probability": 0.55615234375}, {"start": 1275.96, "end": 1276.3, "word": " 3", "probability": 0.88232421875}, {"start": 1276.3, "end": 1276.64, "word": " إذا", "probability": 0.5794677734375}, {"start": 1276.64, "end": 1276.82, "word": " كان", "probability": 0.97705078125}, {"start": 1276.82, "end": 1276.94, "word": " الـ", "probability": 0.7783203125}, {"start": 1276.94, "end": 1277.4, "word": " Preceptive", "probability": 0.9060872395833334}, {"start": 1277.4, "end": 1277.9, "word": " بسيط", "probability": 0.8907470703125}, {"start": 1277.9, "end": 1278.22, "word": " plus", "probability": 0.53369140625}, {"start": 1278.22, "end": 1278.68, "word": " 1", "probability": 0.94287109375}, {"start": 1278.68, "end": 1279.42, "word": " إذا", "probability": 0.8974609375}, {"start": 1279.42, "end": 1279.9, "word": " واصلت", "probability": 0.74169921875}, {"start": 1279.9, "end": 1280.32, "word": " plus", "probability": 0.9619140625}, {"start": 1280.32, "end": 1280.7, "word": " 2", "probability": 0.86767578125}], "temperature": 1.0}, {"id": 51, "seek": 130540, "start": 1281.94, "end": 1305.4, "text": "هو أسجل النتيجة المفروض أن حياتيه برقم 5 هي اللي تطلع عندنا أعلى precipitate إذا طلع عندنا خطأ بنحسب نسبة الخطأ الـError اللي هي الـExperimental Value ناقص الـTheoretical Value على الـTheoretical Value ضارب 100 اللي هي القيمة المحسوبة ناقص القيمة العملية اللي أنا أشتغلتها على القيمة المحسوبة ضارب 100", "tokens": [3224, 2407, 5551, 3794, 7435, 1211, 28239, 31371, 7435, 3660, 9673, 5172, 32887, 11242, 14739, 11331, 1829, 9307, 1829, 3224, 4724, 2288, 4587, 2304, 1025, 39896, 13672, 1829, 6055, 9566, 1211, 3615, 43242, 8315, 5551, 3615, 23942, 23354, 8086, 11933, 15730, 23032, 1211, 3615, 43242, 8315, 16490, 9566, 10721, 44945, 5016, 35457, 8717, 35457, 3660, 33962, 9566, 10721, 2423, 39184, 28135, 2874, 13672, 1829, 39896, 2423, 39184, 11149, 610, 2328, 304, 39352, 8717, 995, 4587, 9381, 2423, 39184, 2278, 26262, 804, 39352, 15844, 2423, 39184, 2278, 26262, 804, 39352, 48812, 9640, 3555, 2319, 13672, 1829, 39896, 25062, 32640, 3660, 9673, 5016, 3794, 37746, 3660, 8717, 995, 4587, 9381, 25062, 32640, 3660, 18863, 42213, 10632, 13672, 1829, 41850, 5551, 8592, 2655, 17082, 1211, 2655, 11296, 15844, 25062, 32640, 3660, 9673, 5016, 3794, 37746, 3660, 48812, 9640, 3555, 2319], "avg_logprob": -0.17164855731138284, "compression_ratio": 1.9357429718875503, "no_speech_prob": 3.707408905029297e-05, "words": [{"start": 1281.94, "end": 1282.24, "word": "هو", "probability": 0.762451171875}, {"start": 1282.24, "end": 1282.62, "word": " أسجل", "probability": 0.751739501953125}, {"start": 1282.62, "end": 1283.26, "word": " النتيجة", "probability": 0.9510498046875}, {"start": 1283.26, "end": 1284.3, "word": " المفروض", "probability": 0.982666015625}, {"start": 1284.3, "end": 1284.44, "word": " أن", "probability": 0.49365234375}, {"start": 1284.44, "end": 1285.0, "word": " حياتيه", "probability": 0.6844970703125}, {"start": 1285.0, "end": 1285.4, "word": " برقم", "probability": 0.87451171875}, {"start": 1285.4, "end": 1285.8, "word": " 5", "probability": 0.67919921875}, {"start": 1285.8, "end": 1286.06, "word": " هي", "probability": 0.86376953125}, {"start": 1286.06, "end": 1286.24, "word": " اللي", "probability": 0.902587890625}, {"start": 1286.24, "end": 1286.6, "word": " تطلع", "probability": 0.9674072265625}, {"start": 1286.6, "end": 1286.92, "word": " عندنا", "probability": 0.4874267578125}, {"start": 1286.92, "end": 1287.34, "word": " أعلى", "probability": 0.9337565104166666}, {"start": 1287.34, "end": 1288.24, "word": " precipitate", "probability": 0.65283203125}, {"start": 1288.24, "end": 1288.82, "word": " إذا", "probability": 0.880859375}, {"start": 1288.82, "end": 1289.06, "word": " طلع", "probability": 0.9601236979166666}, {"start": 1289.06, "end": 1289.34, "word": " عندنا", "probability": 0.845458984375}, {"start": 1289.34, "end": 1289.66, "word": " خطأ", "probability": 0.9886067708333334}, {"start": 1289.66, "end": 1290.18, "word": " بنحسب", "probability": 0.8902994791666666}, {"start": 1290.18, "end": 1290.72, "word": " نسبة", "probability": 0.9908854166666666}, {"start": 1290.72, "end": 1291.28, "word": " الخطأ", "probability": 0.94189453125}, {"start": 1291.28, "end": 1291.94, "word": " الـError", "probability": 0.6241455078125}, {"start": 1291.94, "end": 1292.7, "word": " اللي", "probability": 0.6552734375}, {"start": 1292.7, "end": 1292.92, "word": " هي", "probability": 0.693359375}, {"start": 1292.92, "end": 1294.84, "word": " الـExperimental", "probability": 0.7987060546875}, {"start": 1294.84, "end": 1295.24, "word": " Value", "probability": 0.80810546875}, {"start": 1295.24, "end": 1295.88, "word": " ناقص", "probability": 0.9444580078125}, {"start": 1295.88, "end": 1296.52, "word": " الـTheoretical", "probability": 0.92080078125}, {"start": 1296.52, "end": 1297.02, "word": " Value", "probability": 0.9609375}, {"start": 1297.02, "end": 1297.64, "word": " على", "probability": 0.818359375}, {"start": 1297.64, "end": 1298.4, "word": " الـTheoretical", "probability": 0.90810546875}, {"start": 1298.4, "end": 1298.86, "word": " Value", "probability": 0.97607421875}, {"start": 1298.86, "end": 1299.38, "word": " ضارب", "probability": 0.8323567708333334}, {"start": 1299.38, "end": 1299.76, "word": " 100", "probability": 0.8125}, {"start": 1299.76, "end": 1300.04, "word": " اللي", "probability": 0.927734375}, {"start": 1300.04, "end": 1300.14, "word": " هي", "probability": 0.9296875}, {"start": 1300.14, "end": 1300.5, "word": " القيمة", "probability": 0.8806966145833334}, {"start": 1300.5, "end": 1301.08, "word": " المحسوبة", "probability": 0.96123046875}, {"start": 1301.08, "end": 1301.4, "word": " ناقص", "probability": 0.9593505859375}, {"start": 1301.4, "end": 1301.8, "word": " القيمة", "probability": 0.96337890625}, {"start": 1301.8, "end": 1302.24, "word": " العملية", "probability": 0.93408203125}, {"start": 1302.24, "end": 1302.38, "word": " اللي", "probability": 0.9326171875}, {"start": 1302.38, "end": 1302.5, "word": " أنا", "probability": 0.72802734375}, {"start": 1302.5, "end": 1303.06, "word": " أشتغلتها", "probability": 0.9151785714285714}, {"start": 1303.06, "end": 1303.22, "word": " على", "probability": 0.8125}, {"start": 1303.22, "end": 1303.6, "word": " القيمة", "probability": 0.9666341145833334}, {"start": 1303.6, "end": 1304.24, "word": " المحسوبة", "probability": 0.99228515625}, {"start": 1304.24, "end": 1304.68, "word": " ضارب", "probability": 0.9578450520833334}, {"start": 1304.68, "end": 1305.4, "word": " 100", "probability": 0.88427734375}], "temperature": 1.0}], "language": "ar", "language_probability": 1.0, "duration": 1309.3965, "duration_after_vad": 1093.3974999999987}
\ No newline at end of file
diff --git a/PL9fwy3NUQKwZ1Q7zOg1ObgufPMjRI3WY1/zJp2OlvRd8A_raw.srt b/PL9fwy3NUQKwZ1Q7zOg1ObgufPMjRI3WY1/zJp2OlvRd8A_raw.srt
new file mode 100644
index 0000000000000000000000000000000000000000..b30e0a98cfd13802791123ae44634cb839d62078
--- /dev/null
+++ b/PL9fwy3NUQKwZ1Q7zOg1ObgufPMjRI3WY1/zJp2OlvRd8A_raw.srt
@@ -0,0 +1,1040 @@
+1
+00:00:03,620 --> 00:00:06,680
+أو وين بيدوب وين بيكون soluble وين بيكون
+
+2
+00:00:06,680 --> 00:00:09,900
+precipitate يذا فاكرين لما حكينا عن ال ionization
+
+3
+00:00:09,900 --> 00:00:13,060
+عن ال amino acid كيف بيتصرف في الأوساط المختلفة
+
+4
+00:00:13,060 --> 00:00:16,480
+لما حاطنا فيه alkaline و فيه neutral و فيه acidic
+
+5
+00:00:16,480 --> 00:00:21,000
+pHنفسها للبروتين، لأن البروتين هو عبارة عن سلسلة
+
+6
+00:00:21,000 --> 00:00:24,320
+من الأحماض الأمينية أنا لو جبت بروتين وحطيته في
+
+7
+00:00:24,320 --> 00:00:28,140
+الـ neutral pH الـ neutral pH حكينا شو بتكون ال
+
+8
+00:00:28,140 --> 00:00:31,220
+net charge في الأمين واسد، بتكون Zero ال net
+
+9
+00:00:31,220 --> 00:00:35,240
+charge Zero يعني عدد الشحنات الموجبة بتساوي عدد
+
+10
+00:00:35,240 --> 00:00:39,240
+الشحنات السالبة، فإذا كان عدد الشحنات فيها نموجة
+
+11
+00:00:39,240 --> 00:00:43,020
+وفيها سالبة، بتكون في ال neutral pHفي شحنات موجبة
+
+12
+00:00:43,020 --> 00:00:48,500
+وفي شحنات سالبة الشحنات المختلفة تتجادب مع بعضها
+
+13
+00:00:48,500 --> 00:00:52,240
+معروف عن الشحنات المختلفة أنها تتجادب و الشحنات
+
+14
+00:00:52,240 --> 00:00:56,300
+المتشابهة تتنافس في الـ neutral بيكون فيه موجب و
+
+15
+00:00:56,300 --> 00:01:00,980
+فيه سالب فبيتجادبوا مع بعض بسبب aggregation أو
+
+16
+00:01:00,980 --> 00:01:05,450
+precipitationفإذا الوين هيترسّب البروتين عند الـ
+
+17
+00:01:05,450 --> 00:01:08,690
+neutral pH اللي هي بتكون فيها الـ pH عند الـ
+
+18
+00:01:08,690 --> 00:01:12,950
+isoelectric point بتساوي الـ isoelectric point
+
+19
+00:01:12,950 --> 00:01:17,330
+فنقطة الـ isoelectric point هيترسّب البروتين لكن
+
+20
+00:01:17,330 --> 00:01:22,410
+مثلا لو حطته في acidic media يعني الـ pH كانت أقل
+
+21
+00:01:22,410 --> 00:01:27,100
+من الـ isoelectric pointacidic يعني pH أقل من الـ
+
+22
+00:01:27,100 --> 00:01:31,300
+isoelectric bond حكينا الشحنات بتكون موجبة بتكون
+
+23
+00:01:31,300 --> 00:01:35,260
+ال net charge موجبة في ال acidic ال net charge
+
+24
+00:01:35,260 --> 00:01:38,840
+بتكون موجبة الأحمد الأمينية المكونة للبروتين هتكون
+
+25
+00:01:38,840 --> 00:01:43,010
+شحنتها موجبةيعني شحنات متشابهة positive charge
+
+26
+00:01:43,010 --> 00:01:47,370
+الشحنات المتشابهة إيش هي بيصير بينها؟ بيصير تنافر
+
+27
+00:01:47,370 --> 00:01:52,550
+تنافر و بتبعد عن بعضها و بتتوزع فيه المحلول فبتصير
+
+28
+00:01:52,550 --> 00:01:56,310
+soluble ما بتترسب و انما بيصير soluble طب لو
+
+29
+00:01:56,310 --> 00:02:01,250
+حطيتها في alkaline ميلياجيبت هذا البروتين مكونة من
+
+30
+00:02:01,250 --> 00:02:04,770
+أحمض أمينية حاطته في الـAlkaline يعني الـBH أعلى
+
+31
+00:02:04,770 --> 00:02:08,990
+من الـBi الـBH بتكون أعلى من الـBi على الـAlkaline
+
+32
+00:02:08,990 --> 00:02:12,950
+لإن الـBhi نتش هاجحة كده بتكون في الـAlkaline
+
+33
+00:02:12,950 --> 00:02:16,770
+نيجاتيف فكل الشحنات اللي على أحمض الأمينية نيجاتيف
+
+34
+00:02:16,770 --> 00:02:21,710
+شحنات متشابهة، إيش بيصير بينها؟ بتتنافر، بتتنافر و
+
+35
+00:02:21,710 --> 00:02:26,290
+بتتوزع فيه المحلول وبالتالي بتصير soluble، بتكون
+
+36
+00:02:26,290 --> 00:02:30,800
+soluble مش ابتتجادالو جينا قرا عن هذه النقاط نفس
+
+37
+00:02:30,800 --> 00:02:34,780
+اللي حكيناها تحكيلي ال solution pH is above the PI
+
+38
+00:02:34,780 --> 00:02:38,780
+ال pH أعلى من ال PI مدام ال pH أعلى يعني أنا وصلت
+
+39
+00:02:38,780 --> 00:02:42,300
+ل alkaline media في ال alkaline ال charge، ال net
+
+40
+00:02:42,300 --> 00:02:44,260
+charge بتكون negatively charged
+
+41
+00:02:49,890 --> 00:02:53,510
+الشحنات المتشابهة لـ like charge عندها قوة تنافر
+
+42
+00:02:53,510 --> 00:02:57,530
+لذلك البروتين والبولسوليو بالادس BH فالبروتين
+
+43
+00:02:57,530 --> 00:03:03,330
+هيكون ذائب likewise برضه بنفس الطريقة لما تكون
+
+44
+00:03:03,330 --> 00:03:07,430
+البروتين positive charge وين بيكون positive charge
+
+45
+00:03:07,430 --> 00:03:13,430
+لما تكون ال BH أقل من الBI اللي في acidic BHبيكون
+
+46
+00:03:13,430 --> 00:03:17,070
+برضه عندنا شحنات متشابهة بتصير في بينهم repulsion
+
+47
+00:03:17,070 --> 00:03:22,010
+قوة تنافذ فبيكون البروتين والـB سوليو بالاديس this
+
+48
+00:03:22,010 --> 00:03:26,710
+BH التبيئة عند الـ isoelectric point بالظبط اللي
+
+49
+00:03:26,710 --> 00:03:30,150
+هي عند الـ neutral BH هي نقطة الـ isoelectric
+
+50
+00:03:30,150 --> 00:03:33,170
+point بيكون عندنا negative و positive charge
+
+51
+00:03:33,170 --> 00:03:37,610
+negative و positive هيقلل ال repulsive repulsive
+
+52
+00:03:37,610 --> 00:03:42,320
+أو electrostatic or reduceفماذا سيحصل؟ هو الـ
+
+53
+00:03:42,320 --> 00:03:44,500
+Cause Aggregation and Precipitation
+
+54
+00:03:48,230 --> 00:03:52,130
+مع بعض تتجمع ويصير precipitation أو aggregation
+
+55
+00:03:52,130 --> 00:03:56,150
+للبروتين فإذا أنا بدي أدوب أي بروتين أنا أعطيتك
+
+56
+00:03:56,150 --> 00:03:59,930
+حاجات لك دا و بيلي هذا البروتين بدور على نقطة الـ
+
+57
+00:03:59,930 --> 00:04:02,490
+isoelectric point له بكون عارفة نقطة الـ
+
+58
+00:04:02,490 --> 00:04:06,910
+isoelectric point بجيب مثلا buffer يضبطلي الـ pH
+
+59
+00:04:06,910 --> 00:04:09,690
+عند هذه الـ isoelectric point ويدوب البروتين
+
+60
+00:04:09,690 --> 00:04:14,130
+بيحكيلي الـ isoelectric point لمعظم البروتين بتكون
+
+61
+00:04:14,130 --> 00:04:18,610
+بين pH 4الى 6
+
+62
+00:04:22,760 --> 00:04:27,400
+إذا بدي أعمل أني أرسب أي بروتين أسف على المعلومة
+
+63
+00:04:27,400 --> 00:04:31,200
+اللي جابل إذا بدي أرسب أي بروتين مش أدوب أي بروتين
+
+64
+00:04:31,200 --> 00:04:36,900
+بضبط ال pH عند نقطة ال PI إذا بدي أرسبه بضبط ال pH
+
+65
+00:04:36,900 --> 00:04:40,780
+عند ال PI أما إذا بدي أدوبه بدي أرفع ال pH أو
+
+66
+00:04:40,780 --> 00:04:44,420
+أوصلها لل acid أو بدي أقلل .. بدي أقللها أووصلها
+
+67
+00:04:44,420 --> 00:04:48,370
+لل acid أو أرفعها أني أوصلها لل alkalineلكن إذا
+
+68
+00:04:48,370 --> 00:04:53,570
+بدي أرسبه بدي أضبط ال bh عند ال bi مش إني أدوبه
+
+69
+00:04:53,570 --> 00:05:03,370
+ماذا هيترسب إذا ضبطت ال bh عند ال bi احكيلي
+
+70
+00:05:03,370 --> 00:05:07,810
+أنه solubility of ال protein ثقابية ال protein في
+
+71
+00:05:07,810 --> 00:05:10,630
+بعض ال protein ثقابيتها لا تتأثر بالحرارة يعني
+
+72
+00:05:10,630 --> 00:05:14,870
+حاليا احكيلي بدي أدوبه بالحرارةلايتأثر بالحرارة
+
+73
+00:05:14,870 --> 00:05:19,110
+بسبب تركيبته زي الـ Casein فأنا كيف بدي أزوج وصف
+
+74
+00:05:19,110 --> 00:05:22,270
+الـ ability of casein؟ تحتمل على الـ pH، يعني أوصل
+
+75
+00:05:22,270 --> 00:05:27,910
+الـ pH للـ BI يعني أبط الـ pH توصل للـ isoelectric
+
+76
+00:05:27,910 --> 00:05:31,990
+point لأن حكينا لما متى ما كانت الـ pH بتساوي الـ
+
+77
+00:05:31,990 --> 00:05:36,510
+BI، يترسب الـ protein فإذا أنا بدي أرسبه، بدي ألعب
+
+78
+00:05:36,510 --> 00:05:39,670
+في الـ pH إذا بدي أدوبه، برفع الـ pH، بخليه أعلىمن
+
+79
+00:05:39,670 --> 00:05:43,370
+ال بي اي او اقل من ال بي اي يكون في ال acidity او
+
+80
+00:05:43,370 --> 00:05:47,550
+ال alkalinity هذا بيدوم بس اذا وصلت ال بي اتش لل
+
+81
+00:05:47,550 --> 00:05:52,530
+بي اي بدي يتحسن ال intermediate pH at which
+
+82
+00:05:52,530 --> 00:05:55,370
+protein molecule has charge of 0 نسميها ال
+
+83
+00:05:55,370 --> 00:05:58,590
+isoelectric point حتى هنا ان ال isoelectric point
+
+84
+00:05:58,590 --> 00:06:03,130
+هي قيمة بي اتش اللي عندها بتكون ان انت charge 0عند
+
+85
+00:06:03,130 --> 00:06:07,190
+هذه النقطة، يعني نقطة الـ isoelectric point دائمية
+
+86
+00:06:07,190 --> 00:06:11,590
+البروتين تكون أقل ما يمكن، solubility is minimal
+
+87
+00:06:11,590 --> 00:06:17,110
+كيف بدأ أزود الدائمية؟ أني يا إما بأزود الـ pH يا
+
+88
+00:06:17,110 --> 00:06:21,530
+بقلل الـ pH حكينا إن كانت الـ pH أعلى من الـ PI أو
+
+89
+00:06:21,530 --> 00:06:25,030
+أقل من الـ PI، بكون soluble بس متى ما كانت عند الـ
+
+90
+00:06:25,030 --> 00:06:30,180
+PI، بيكون precipitatePrecipitation or coagulation
+
+91
+00:06:30,180 --> 00:06:33,500
+of milk protein casein at low pH as milk becomes
+
+92
+00:06:33,500 --> 00:06:37,200
+white بيحكي الفيلم إنها تطبيق على ترسيب البروتين
+
+93
+00:06:37,200 --> 00:06:41,800
+له فساد الحليب، فيه هنا ظاهرة، ظاهرة فساد الحليب
+
+94
+00:06:41,800 --> 00:06:44,860
+كيف فساد الحليب؟ فالجهة الحليب معروفة عنه إن هو
+
+95
+00:06:44,860 --> 00:06:48,820
+وسطه قاعي تمام؟ ومن مكونات الحليب فيه عدة
+
+96
+00:06:48,820 --> 00:06:51,960
+بروتينات، منها البروتين الكازيني وجدوا إن
+
+97
+00:06:51,960 --> 00:06:55,840
+الـisoelectric point للكازين هي حوالي أربعة وسبعة
+
+98
+00:06:57,200 --> 00:07:01,780
+وهي أقل الـ 4.7 أقل من الـ pH بتاعة الحليب فجد
+
+99
+00:07:01,780 --> 00:07:05,960
+الحليب وسط قاي فبال isoelectric point للكازين اللي
+
+100
+00:07:05,960 --> 00:07:10,660
+موجود في الحليب 4.7 مين أعلى ال pH قاي ولا ال 4.7
+
+101
+00:07:10,660 --> 00:07:14,980
+لأ ال pH لذن ال pH للحليب أعلى من ال PI يعني
+
+102
+00:07:14,980 --> 00:07:18,540
+الشحنة هتكون ال net charge negative فهيكون ال
+
+103
+00:07:18,540 --> 00:07:20,000
+protein that kept بتكون في الحليب
+
+104
+00:07:25,940 --> 00:07:29,740
+الكزين اللي موجود في الحليب اربعة وسبعة مينها على
+
+105
+00:07:29,740 --> 00:07:31,760
+البيئة توقعي ولا الاربعة وسبعة
+
+106
+00:07:46,290 --> 00:07:51,150
+سالبة فبتصير طنافر فهيكون سلبة لما يصير فساد
+
+107
+00:07:51,150 --> 00:07:55,190
+للحليب يلمع فيه أورجانيزم يتم تلمع فيه بكتيريا مش
+
+108
+00:07:55,190 --> 00:07:58,970
+هاي الترمال بتحول سكر اللاكتوز الموجود في الحليب ل
+
+109
+00:07:58,970 --> 00:08:04,210
+Lactic Acid و Lactic Acid هذا حمق لأن هو وسط قاعد
+
+110
+00:08:04,210 --> 00:08:07,230
+الحليب مع ال بكتيريا اللي نمت فيه هو القوانات
+
+111
+00:08:07,230 --> 00:08:11,730
+للاكتك أسد Lactic Acid هذا هيقلل البيئات اللي هو
+
+112
+00:08:11,730 --> 00:08:14,650
+اللي بيقللها بيقللها وصولا إلى الأرض
+
+113
+00:08:18,250 --> 00:08:22,230
+مجد خسارة الحليب هو الـ Lactic Acid وهو ربعاً حمق
+
+114
+00:08:22,230 --> 00:08:27,970
+والحليب أصلا وسط القاعدة فجد قاعدة محمق بيقلل الـ
+
+115
+00:08:27,970 --> 00:08:34,550
+pH من قاعدة الحليب وصولا إلى درجة الحموظة فبتقترب
+
+116
+00:08:34,550 --> 00:08:40,470
+من الـBi للكازين اللي هي 4 و7 مدام الـBi وصلت
+
+117
+00:08:40,470 --> 00:08:45,070
+الـBH صاروا متساويين إذا بيترسب الكازين فبيصير فيه
+
+118
+00:08:45,070 --> 00:08:46,590
+تنسيق للكازين
+
+119
+00:09:13,180 --> 00:09:17,340
+فكيف بتعرف الـ isoelectric point للكازين؟ بناء على
+
+120
+00:09:17,340 --> 00:09:20,960
+ال principle اللي هو المبدأ إنه بدي أستخدم أسيتات
+
+121
+00:09:20,960 --> 00:09:26,720
+buffer بستخدم buffer اللي قيم BH مختلفة عشان أكشف
+
+122
+00:09:26,720 --> 00:09:29,740
+عن الـ isoelectric point of كازين يعني أنا بدي
+
+123
+00:09:29,740 --> 00:09:33,740
+ألعب بالـ BH بغير الـ BH حكيمة مدى ما بدي أعرف الـ
+
+124
+00:09:33,740 --> 00:09:37,840
+isoelectric point بعرف بغير الـ BH مثلا بستخدم
+
+125
+00:09:37,840 --> 00:09:42,280
+تسعة نبيب و أحط عليهم روتين الكازين وكل نبوبة فيها
+
+126
+00:09:54,070 --> 00:09:58,670
+الأول تانية تلاتة و تمانية بعدها اربعة و واحد اربع
+
+127
+00:09:58,670 --> 00:10:03,350
+واربع اربع وسبعة خمسة خمسة و تلاتة خمسة و ستة خمسة
+
+128
+00:10:03,350 --> 00:10:07,470
+و جسد عنده قيم بيقات مختلفة تمام انا استخدمت هذه
+
+129
+00:10:07,470 --> 00:10:13,050
+القنادير و حطيت فيهم كمية من الحمب بتركيز مختلفة
+
+130
+00:10:29,150 --> 00:10:33,990
+معادلة بحسم من خلالها دياتي أنا عندى عدة قنابيل
+
+131
+00:10:33,990 --> 00:10:38,590
+تحتوي على protein الكازين ومحطوط معاها buffer ال
+
+132
+00:10:38,590 --> 00:10:43,470
+buffer هذه تعمل من حمض وقاعد تركيز الحمض مختلفة
+
+133
+00:10:43,470 --> 00:10:47,410
+أنا عاملانا تخفيف كل انبوب عن التعنى ليش؟ عشان
+
+134
+00:10:47,410 --> 00:10:51,470
+أغير البيات بعدين بنسك هذه القنابيل باخد الانبوب
+
+135
+00:10:51,470 --> 00:10:51,950
+الأول
+
+136
+00:11:10,940 --> 00:11:14,340
+الأنبوبة المترسّب فيها البروتين الكازيين من هذه
+
+137
+00:11:14,340 --> 00:11:17,180
+الأنبوبة هي قيمة الـ pH
+
+138
+00:11:42,230 --> 00:11:47,330
+الـ BKA للي
+
+139
+00:11:47,330 --> 00:11:55,310
+احنا مستخدمينها هي معروفة 4.75 BKA 4.75 ال base
+
+140
+00:11:55,310 --> 00:11:58,290
+القاعد اللي أنا استخدمتها اللي بستخدمها في التجربة
+
+141
+00:11:58,290 --> 00:12:02,690
+هي كازين أسيتات صوبيوم مش مفهوم منكوا تعرفوا
+
+142
+00:12:11,800 --> 00:12:15,740
+الكزين أسيتات Sodium الـ Milk Base تركيزها 1 من 10
+
+143
+00:12:15,740 --> 00:12:19,460
+مين اللي غير تركيزه الـ Acetic Acid فكل أنفور
+
+144
+00:12:19,460 --> 00:12:25,200
+هيكون له تركيز لبحن مختلف عن التاني عشان يتغير قيم
+
+145
+00:12:25,200 --> 00:12:30,220
+البيعات معروفة أن هذه التركيز موجودة في آخر في قبل
+
+146
+00:12:30,220 --> 00:12:33,220
+الأخيرة سواء في قبل الأخيرة في جدول موجودة
+
+147
+00:12:35,490 --> 00:12:39,350
+جداش تركيزة في النبوب الأول مثلا 1 و 6، للنبوب
+
+148
+00:12:39,350 --> 00:12:43,090
+التالت 8 من 10، للنبوب التالت 4 من 10 و هكذا، هذه
+
+149
+00:12:43,090 --> 00:12:47,030
+محسوبة اللي هي تركيز الحمض، بحطها في المعادلة، صار
+
+150
+00:12:47,030 --> 00:12:51,430
+عندي معروف تركيز الـBKA جداش، والبس 1 من 10،
+
+151
+00:12:51,430 --> 00:12:54,590
+والacetic acid بتاخدها من الجدول، واحسب الـBH
+
+152
+00:12:54,590 --> 00:12:58,670
+للنبوب الأول، BH للنبوب التاني، للتالت، فهذا
+
+153
+00:12:58,670 --> 00:13:03,660
+الجدول اللي في الأخرهو تركيز الحمض في الجداش في كل
+
+154
+00:13:03,660 --> 00:13:10,020
+انبوبة وال pH جداش موجودة لكل محسوبة لكل انبوبة
+
+155
+00:13:10,020 --> 00:13:12,780
+كيف حسبها من خلال المعادلة اللي فوق اللي طبعا
+
+156
+00:13:12,780 --> 00:13:18,100
+حكينا عنها
+
+157
+00:13:18,100 --> 00:13:22,440
+اكررنا ان حكينا ان البروتينات عشان اخليها تترسب
+
+158
+00:13:22,440 --> 00:13:26,460
+لازم اوصيها لل isoelectric bond to aggregate and
+
+159
+00:13:26,460 --> 00:13:27,120
+dissipate
+
+160
+00:13:32,930 --> 00:13:35,510
+أحنا مختلفة تتجادل مع بعضها
+
+161
+00:13:47,940 --> 00:13:51,160
+حسب مين؟ حسب الـ Amino Acid Sequence حسب عدد الـ
+
+162
+00:13:51,160 --> 00:13:55,060
+Anionic والكاتيونيك الـ Growth فأنا بحكي لي هدفات
+
+163
+00:13:55,060 --> 00:14:00,100
+بستخدمها و بستفيد منها لو بدي أفصل البروتين من عدة
+
+164
+00:14:00,100 --> 00:14:03,320
+بروتينات يعني مثلا عندي حليب يحتوي على عدة
+
+165
+00:14:03,320 --> 00:14:07,420
+بروتينات من ضمنها البروتين الكازين كيف بدي أخد من
+
+166
+00:14:07,420 --> 00:14:11,200
+الحليب بس البروتين الكازين و أسيب الباقي؟ بعرف
+
+167
+00:14:11,200 --> 00:14:15,220
+الـBI للكازين و بوصلها الـBH للـBI
+
+168
+00:14:35,740 --> 00:14:42,140
+ساوى للـ VI هالوراسة في تجربتنا لليوم مش البدوات
+
+169
+00:14:42,140 --> 00:14:45,560
+اللى بدي استخدمها بستخدم acidic acid اللى هو الحمض
+
+170
+00:14:45,560 --> 00:14:48,480
+بستخدم البروتين الكازين اللى هو اللى أنا بدورله
+
+171
+00:14:48,480 --> 00:14:50,980
+على ال isoelectric point اللى بتعرفه isoelectric
+
+172
+00:14:50,980 --> 00:14:54,760
+point بستخدم NA watch عشان ارفع البيئات عشان
+
+173
+00:14:54,760 --> 00:14:58,260
+اوصلها على القاعدة الحمض بدي نزل البيئات NA watch
+
+174
+00:14:58,260 --> 00:14:58,920
+بيرفع
+
+175
+00:15:04,450 --> 00:15:09,090
+عشان أعمل dilution و أغير قيم الـ pH عشان أتغير و
+
+176
+00:15:09,090 --> 00:15:12,950
+أعرف أن الـ pH تساوي الـ isoelectric bond و بدي
+
+177
+00:15:12,950 --> 00:15:17,430
+أستخدم تسع تيوبات ليش تسع تيوبات؟ عشان كل تيوب
+
+178
+00:15:17,430 --> 00:15:21,470
+تكون فيها قيمة الـ pH مختلفة عن التانية عشان أعرف
+
+179
+00:15:21,470 --> 00:15:30,150
+وين هو ترثبت أي pH من هذه التيوبات كيف
+
+180
+00:15:30,150 --> 00:15:33,290
+حطوات إجراء التجربة؟ أول شيء مضاجينه
+
+181
+00:15:38,210 --> 00:15:42,510
+عارفة من فوق تقولها زي رجب من فوق ساعتها تكون
+
+182
+00:15:42,510 --> 00:15:45,550
+خمسين مل اذا حضر فيها ال buffer اللي انا بدي
+
+183
+00:15:45,550 --> 00:15:49,630
+استخدمه ايش بدي احط فيها كيف بدي احضر ال buffer
+
+184
+00:15:49,630 --> 00:15:54,210
+بحط عشرين مل من ديستل واتر على خمسة وعشرين من مية
+
+185
+00:15:54,210 --> 00:16:01,110
+جرام كزينعلى خمسة مل نا واتش وخمسة مل أسيتك أسد
+
+186
+00:16:01,110 --> 00:16:05,010
+وعشرين مل ديستل واتر يعني أنا بحط البروتين اللي
+
+187
+00:16:05,010 --> 00:16:08,150
+أنا بدورله على ال isoelectric point مع أسد و base
+
+188
+00:16:08,150 --> 00:16:11,150
+مش ال buffer هو عبارة عن أسد مع conjugate base
+
+189
+00:16:11,150 --> 00:16:16,230
+أضيفهم عليه و ديستل واتر عشان أدورهم فيهو اعمل mix
+
+190
+00:16:16,230 --> 00:16:19,410
+هان اتكون عندي ال buffer اللي هو عبارة عن ال base
+
+191
+00:16:19,410 --> 00:16:25,290
+بتركيز 1 من 10 بحضره و بحطه على جنب و بعدين بجيب 9
+
+192
+00:16:25,290 --> 00:16:31,770
+تيوبات منابيب برقمهم من 1 ل 9 نحط فيهم دولة
+
+193
+00:16:31,770 --> 00:16:35,970
+distilled water اول اشي بتقضيف عليها تيوب رقم 1
+
+194
+00:16:35,970 --> 00:16:41,920
+distilled water مقدار 6.8وبعدين القنابيب من رقم 2
+
+195
+00:16:41,920 --> 00:16:47,020
+ل 9 بتضيف عليهم 5 مل ديسترواتا رقم 1 بتضيف عليه 86
+
+196
+00:16:47,020 --> 00:16:51,480
+مل ديسترواتا أما القنابيب من 2 ل 9 بتضيف عليهم 5
+
+197
+00:16:51,480 --> 00:16:56,120
+مل ديسترواتا بعدين بتضيف عليه acidic acid اللي هو
+
+198
+00:16:56,120 --> 00:17:00,880
+الحمد والحمد هذا بتضيفه بتركيز مختلفة عشان أغير
+
+199
+00:17:00,880 --> 00:17:07,870
+قيم ال pH لكل tube ف tube رقم 1 تضيف عليها 3 و 2من
+
+200
+00:17:07,870 --> 00:17:11,550
+الـ acetic acid تلاتة و اتنين في تلاتة فاصلة اتنين
+
+201
+00:17:11,550 --> 00:17:15,370
+من الحمض من الحمض من ال acetic acid لأن نبيب
+
+202
+00:17:15,370 --> 00:17:18,490
+التاني ماليش يحط عليها من الحمض كيف بدي اعمل بتاخد
+
+203
+00:17:18,490 --> 00:17:22,940
+من انبوب واحد خمسة ميل على انبوب اتنينأبوب واحد
+
+204
+00:17:22,940 --> 00:17:26,320
+صار فيه ستة و تمانية ديستل واتر و تلاتة و اتنين
+
+205
+00:17:26,320 --> 00:17:30,340
+راسيتي كهسان أما باجي الأناديفش فيها خمسة مل من
+
+206
+00:17:30,340 --> 00:17:34,120
+ديستل واتر بدي اجي على أبوب واحد اللي فيه ديستل
+
+207
+00:17:34,120 --> 00:17:41,460
+واتر محاني بدي اعمل تخفيفات للحم فبدي اخدعامل mix
+
+208
+00:17:41,460 --> 00:17:48,120
+واخد خمسة مل من انبوب تلاتة
+
+209
+00:17:48,120 --> 00:17:52,800
+لاربع وعامل mix واخد خمسة مل من انبوب اربع لخمسة
+
+210
+00:17:52,800 --> 00:17:58,370
+وخمسة مل من خمسة لستة واخد خمسة مل من ستة لسبعةو
+
+211
+00:17:58,370 --> 00:18:01,990
+بعدين خمسة مل من سبعة ل تمانية و بعدين خمسة مل من
+
+212
+00:18:01,990 --> 00:18:06,290
+تمانية لتسعة صارت كل الأنابيب تحتوي على خمسة مل
+
+213
+00:18:06,290 --> 00:18:10,870
+ديستر واطر من رقم اتنين لتسعة تحتوي خمسة مل ديستر
+
+214
+00:18:10,870 --> 00:18:16,150
+واطر و خمسة مل من الأنبوب اللي جابنا عملتلهم mix
+
+215
+00:18:16,150 --> 00:18:19,830
+مع بعض و contango الخمسة فظل المحصلة بس خمسة مل
+
+216
+00:18:19,830 --> 00:18:24,850
+ديستر واطر مع حمض مع بعض هل جيت معادة الأنبوب
+
+217
+00:18:24,850 --> 00:18:29,260
+الأخيرة؟من الأنبوب الأخيرة صار فيها خمسة مل زيادة
+
+218
+00:18:29,260 --> 00:18:32,680
+لإن أنا ما نجلتش لحد بعدها فبدي أطلع منها خمسة مل
+
+219
+00:18:32,680 --> 00:18:36,540
+و أكرها، خلاص بتتخلص منها، أجيت أنا صرت عاملة
+
+220
+00:18:36,540 --> 00:18:40,700
+تخفيفات تدريجيية Serial Delusion للأنبي و للحمة
+
+221
+00:18:40,700 --> 00:18:44,500
+اللي أناضفته للـacetic acidأنا بس على الأول أضفت
+
+222
+00:18:44,500 --> 00:18:48,680
+أستيك أسد و بعدين بلشت أنجل من النبوب 1 ل2، 2 ل3،
+
+223
+00:18:48,680 --> 00:18:53,060
+3 ل4، و هكذا وصلت للآخر الـ TUE فعملت Serial
+
+224
+00:18:53,060 --> 00:18:56,980
+Dilution، تخفيف تدريجي للحم ليش هدى التخفيف؟ عشان
+
+225
+00:18:56,980 --> 00:19:01,400
+أغير قيم الـ pH عشان أعرف وين الـ pH متساوي للـ
+
+226
+00:19:01,400 --> 00:19:07,300
+isoelectric bond بعد هيك باجي بتحط في كل TUE بات
+
+227
+00:19:07,300 --> 00:19:10,880
+من 1 ل 9 من ال buffer اللي أنا حضرته في ال flask
+
+228
+00:19:14,160 --> 00:19:19,180
+بعدين مل حضرت بفر حكاية بتاخد منه واحد مل على تيوب
+
+229
+00:19:19,180 --> 00:19:22,720
+واحد وبعدين من ال flask نفسه واحد مل على تيوب
+
+230
+00:19:22,720 --> 00:19:26,260
+اتنين وبعدين من ال flask نفسه واحد مل على تيوب
+
+231
+00:19:26,260 --> 00:19:30,900
+تلاتي وهكذا لآخر تيوب بتقدر نضيف واحد مل من ال
+
+232
+00:19:30,900 --> 00:19:36,920
+flask على كل تيوبها وبتركه لمدة نص ساعة بترك كل
+
+233
+00:19:36,920 --> 00:19:41,200
+القنابيب لمدة نص ساعة بعد نص ساعة بجلد القنابيب
+
+234
+00:19:41,200 --> 00:19:45,150
+بشوف همسك تيوب تيوب تيوب واحدهمسكها، شوف فيها
+
+235
+00:19:45,150 --> 00:19:48,590
+precipitate تحت ولا مافيش فيها إذا ترسّب، يبقى ال
+
+236
+00:19:48,590 --> 00:19:52,890
+protein هنا مترسّب عند قيمة الـ pH هذه إذا هنا هو
+
+237
+00:19:52,890 --> 00:19:56,570
+بترسّب، إذا هذه هي الـ PR إذا مش مترسّب، بدي أنتج
+
+238
+00:19:56,570 --> 00:20:00,090
+إلى اللي بعدها، بشوف كل الأنابيب، أي أنبوب مترسّب
+
+239
+00:20:00,090 --> 00:20:04,410
+عندها، إذا عند هذه الـ pH متساوي الـ PR
+
+240
+00:20:13,210 --> 00:20:17,230
+أما بيحكي إن هم لما اشتغلوا التجربة وجدوا أن الـ
+
+241
+00:20:17,230 --> 00:20:24,010
+«بي» الـ «بي» الـ isoelectric point للكازين هي 4 و
+
+242
+00:20:24,010 --> 00:20:29,390
+7 يعني المفروض تطلع معناه الـ «به» الـ precipitate
+
+243
+00:20:29,390 --> 00:20:34,350
+للكازين هي «به» 4 و 7 لأن الـ «بي» للكازين هي 4 و
+
+244
+00:20:34,350 --> 00:20:39,410
+7 ففي الجدول أي tube عندها الـ «به» 4 و 7 هي tube
+
+245
+00:20:39,410 --> 00:20:45,680
+رقم 5هو المعنى فيها أعلى precipitates كيف احنا
+
+246
+00:20:45,680 --> 00:20:50,660
+هنسجل النتيجة نحكي ال result ال BKA حكي انها 4.75
+
+247
+00:20:50,660 --> 00:20:54,340
+النتيجة هسجلها همسك تيوب واحد يفترض ان مايكونش
+
+248
+00:20:54,340 --> 00:20:57,440
+فيها precipitates و اكتب عليها negative اتنين
+
+249
+00:20:57,440 --> 00:21:02,280
+مافيش فيها negative تلاتة negative اربع قريبة من
+
+250
+00:21:02,280 --> 00:21:06,940
+ال BH للكزي من ال BY من اربعة وسبعة لكن مش هي
+
+251
+00:21:06,940 --> 00:21:08,860
+فهتكون ممكن تكون plus واحد
+
+252
+00:21:12,560 --> 00:21:15,720
+الـ Precision أعلى لـ Preceptive maximum decision
+
+253
+00:21:15,720 --> 00:21:19,420
+plus 3 إذا كان الـ Preceptive بسيط plus 1 إذا
+
+254
+00:21:19,420 --> 00:21:25,400
+واصلت plus 2هو أسجل النتيجة المفروض أن حياتيه برقم
+
+255
+00:21:25,400 --> 00:21:29,060
+5 هي اللي تطلع عندنا أعلى precipitate إذا طلع
+
+256
+00:21:29,060 --> 00:21:32,920
+عندنا خطأ بنحسب نسبة الخطأ الـError اللي هي
+
+257
+00:21:32,920 --> 00:21:37,020
+الـExperimental Value ناقص الـTheoretical Value
+
+258
+00:21:37,020 --> 00:21:40,500
+على الـTheoretical Value ضارب 100 اللي هي القيمة
+
+259
+00:21:40,500 --> 00:21:43,220
+المحسوبة ناقص القيمة العملية اللي أنا أشتغلتها على
+
+260
+00:21:43,220 --> 00:21:45,400
+القيمة المحسوبة ضارب 100
+