passages.id: split_4_test_1752_passage
passages.type: progene_text
passages.text: Activation with a combination of phytohemagglutinin ( PHA ) and LPS was used to assess secretion of IL-2 , IFN-gamma , IL-4 , IL-10 , soluble IL-2 receptor - alpha ( sIL-2Ralpha ) , IL-1beta , and IL-1R antagonist ( IL-1Ra ) by PBMC in 48 - h cell culture .