passages.id: split_4_test_301_passage
passages.type: progene_text
passages.text: In fibroblasts , immunofluorescence microscopy showed that endogenous Akt / PKB localised to membrane ruffles at the outer edge of the cell following mitogen treatment as did green fluorescent protein ( GFP ) fusions with full - length Akt / PKB or its PH domain alone .