passages.id: split_7_validation_412_passage
passages.type: progene_text
passages.text: To determine whether Tyr(462) is similarly required for Tpo binding , wild - type murine Mpl ( Mpl - WT ) or mutant receptors containing an alanine ( Y462A ) or lysine ( Y462K ) in place of Tyr(462) were expressed in BaF3 cells .