passages.id: split_7_validation_979_passage
passages.type: progene_text
passages.text: Sera from subjects before ( n = 21 ) , at onset ( n = 44 ) , or at increased risk of Type I ( insulin - dependent ) diabetes mellitus ( n = 20 ) and from patients with stiff - man syndrome ( SMS , n = 18 ) or polyendocrine autoimmune syndrome ( PAS , n = 21 ) were analysed for conformational and linear GAD65 epitope recognition by an immunohistochemical blocking test based on human monoclonal GAD65 - ab ( MICA 1 - 10 ) and western blotting of a GAD65 epitope - cDNA - library .