passages.id: split_7_validation_961_passage
passages.type: progene_text
passages.text: While no evidence was found for TNFalpha regulation of the PAI-2 gene through either of these two sites , one of the NF-kappaB - like motifs , transcriptional regulatory motif ( TRM ) , present at position - 400 was found to be essential for constitutive PAI-2 transcription , as mutation of this motif abolished basal PAI-2 promoter activity in both monocyte - like U937 cells and HT1080 fibrosarcoma cells .