passages.id: split_7_validation_1200_passage
passages.type: progene_text
passages.text: Here , we have performed presteady - state kinetic and single molecule motility analyses on three of these mutants [ Y138A , loop 11 triple ( L248A / D249A / E250A ) , and E311A ] that exhibited a similar approximately 3 - fold reduction in both microtubule gliding velocity and microtubule - stimulated ATPase activity .