passages.id: split_9_validation_1616_passage
passages.type: progene_text
passages.text: Evidence suggests that the N - terminal HTH motif of SoxS interacts with a highly conserved region of the soxbox termed recognition element 1 ( RE1 ) , while the C - terminal HTH motif interacts with the less conserved recognition element 2 ( RE2 ) .In the work described here , we prepared a complete library of 101 SoxS mutants containing single alanine substitutions of SoxS , and we characterized the mutant proteins in vivo and in vitro .