News Finding: In addition, they used SABER as an effective tool to identify a genomic element that controls the transcription of a specific gene expressed in a particular type of retinal neuron.
Paper Finding: To successfully deploy a large number of orthogonal concatemers simultaneously using SABER, we also needed to design many orthogonal PER concatemer sequences.
News Context: The team demonstrated SABER to amplify FISH signals at distinct RNA and DNA targets in the different layers of the mouse retina, and visualize up to 17 different target regions on the human X chromosome simultaneously. In addition, they used SABER as an effective tool to identify a genomic element that controls the transcription of a specific gene expressed in a particular type of retinal neuron. "With SABER-amplified FISH analysis and its sensitivity, multiplexing potential, practicality and cost-efficiency, we created a way to overcome key limitations of existing methods and provide researchers with a more powerful handle on a broad spectrum of analyses, ranging from fundamental research, to biomarker discovery and the development of therapeutics," said Wyss Institute Core faculty member Peng Yin, Ph.D., who corresponded the study together with Constance Cepko, Ph.D., the Bullard Professor of Genetics and Neuroscience in the Blavatnik Institute at HMS, and Brian Beliveau, Ph.D., a former Damon Runyon Postdoctoral Fellow in Yin's group and now Assistant Professor at the University of Washington's Department of Genome Sciences in Seattle.
Paper Context: With this Exchange-SABER approach, a multitude of PERconcatemerized probe sets can be hybridized to their targets simultaneously and read out in sequential rounds of imaging (Fig. To successfully deploy a large number of orthogonal concatemers simultaneously using SABER, we also needed to design many orthogonal PER concatemer sequences. 1i ).