News Finding: The authors show that the long non-coding RNA called A-ROD (Activating Regulator Of DKK1 expression) is only functional the moment it is released from chromatin into the nucleoplasm.
Paper Finding: These data are in agreement with a direct transcriptional effect on DKK1 mediated by the long ncRNA A-ROD.
News Context: What is particularly novel in the study is the observtion that the location and quantity of a long non-coding RNA are only as important as how the long non-coding RNA is dynamically distributed within the cell. The authors show that the long non-coding RNA called A-ROD (Activating Regulator Of DKK1 expression) is only functional the moment it is released from chromatin into the nucleoplasm. After its complete release from chromatin, A-ROD is no longer active as an enhancing long non-coding RNA.
Paper Context: We see a significant decrease in total Pol II association at the DKK1 gene body, whereas there is no significant change in total Pol II occupancy at either GAPDH or A-ROD loci, supporting a specific regulatory effect of A-ROD on DKK1 transcription. These data are in agreement with a direct transcriptional effect on DKK1 mediated by the long ncRNA A-ROD. Chromosome conformation to target genes is pre-established.