News Finding: This was chiefly the contribution of Rachel Kadzik, a postdoctoral researcher in Petry's lab and a co-first-author on the paper who purified the g-TuRC and, with Thawani, purified the XMAP215.
Paper Finding: This was surprising because XMAP215 and α β -tubulin directly interact in vitro 23, 24 .
News Context: Their team showed that the same phenomenon was seen in a test tube where g-TuRC generated microtubules together with XMAP215. This was chiefly the contribution of Rachel Kadzik, a postdoctoral researcher in Petry's lab and a co-first-author on the paper who purified the g-TuRC and, with Thawani, purified the XMAP215. Using the pure extractions of each protein, Petry's team was able to see that in a test tube, neither protein can nucleate microtubules without the other.
Paper Context: 7a ). This was surprising because XMAP215 and α β -tubulin directly interact in vitro 23, 24 . We determined that XMAP215 and α β -tubulin interact in vitro using size-exclusion chromatography only under low-salt buffer 23,24 (75 mM NaCl; Supplementary Fig.