News Finding: Importantly, the researchers found that neutralizing annexin-A6 or blocking monocytes during chemotherapy prevents the experimental mammary tumors from metastasizing to the lung.
Paper Finding: Accordingly, Anxa6 KO bEnd.3 cells had defective NF-kB activation and Ccl2 transcription in response to tumour-necrosis factor (TNF) compared with Anxa6 WT cells; notably, neither Csf1 nor Cxcl12 were regulated by ANXA6 ( Supplementary Fig.
News Context: However, De Palma cautions against jumping to conclusions: "While this observation supports the significance of our findings, at the moment we don't know if annexin-A6 has any pro-metastatic activity in human breast cancer". Importantly, the researchers found that neutralizing annexin-A6 or blocking monocytes during chemotherapy prevents the experimental mammary tumors from metastasizing to the lung. These results may help to improve the efficacy and safety of neoadjuvant chemotherapy.
Paper Context: Conversely, PTX-EVs isolated from 4T1-mCh or PyMT-IK1 cells increased NF-kB activity and Ccl2 expression in Rela-proficient bEnd.3 cells; notably, these responses required ANXA6 in EVs. Accordingly, Anxa6 KO bEnd.3 cells had defective NF-kB activation and Ccl2 transcription in response to tumour-necrosis factor (TNF) compared with Anxa6 WT cells; notably, neither Csf1 nor Cxcl12 were regulated by ANXA6 ( Supplementary Fig. Similar to PTX-EV, DOX-EVs from 4T1 cells increased the levels of Ccl2 transcripts (Fig.