News Finding: Because the process closely mimics what happens in living cells, we can use our platform to investigate a range of biological phenomena without having to modify living cells each time."
Paper Finding: S9 ), allowing us to consistently extract the posterior probability distributions of all parameters, which consist of fixed effective dissociation constants of each individual ZF, as well as the effective energies describing ZF-RNAP and ZF-ZF interactions.
News Context: Interestingly, we can restart gene expression outside the cell by feeding the extract with fuel and information, in the form of high-energy phosphates and DNA. Because the process closely mimics what happens in living cells, we can use our platform to investigate a range of biological phenomena without having to modify living cells each time." "Our method can be extended to build fairly complicated systems."
Paper Context: This extension to the model was motivated by our results that a ZF with a fixed binding affinity represses with varying efficiency, depending on the position of the binding site; the changing RNAP-ZF interaction energy therefore provides a simple description of this effect. S9 ), allowing us to consistently extract the posterior probability distributions of all parameters, which consist of fixed effective dissociation constants of each individual ZF, as well as the effective energies describing ZF-RNAP and ZF-ZF interactions. The fits are shown in Fig.