sentence1: To directly measure the effect of endogenous Dnd1 on the activity of endogenous miR-372 family, we used sensor molecules containing the luciferase gene under the control of either wild-type LATS2-3′UTR or a mutant in the 372 target sites
sentence2: The 3′UTRs of p27 and connexin-43 were PCR amplified from genomic DNA and cloned into pGL3 (Promega) downstream of the luciferase gene; constructs bearing the LATS2 3′UTR were described.