sentence1: miR-Vec constructs were described before, and Dnd1 open-reading frames were cloned as described into a pCS2-based CMV expression vector to contain a double carboxy-terminal HA tag.
sentence2: The pMSCV-blast-miR plasmids, containing either hsa-miR-376a1 human miRNA or control miRNA (hTR-human telomerase RNA), were constructed as described previously.