sentence1: T47D, MCF-7, Skbr3, HeLa, and Caco-2 cells were transfected by electroporation as described previously.
sentence2: MCF7 or HeLa cells were electroporated as described previously to more than 95% efficiency with pSuper constructs against the various targets, and 72 hr later, protein expression was analyzed by SDS-PAGE and Western blotting.