fix

cas9on.py

@@ -224,12 +224,12 @@ def create_bigwig(df, bigwig_path):
     bw = pyBigWig.open(bigwig_path, "w")
     bw.addHeader(header)
 
-    # Add entries for each chromosome
-    for chr, group in df.groupby('Chr'):
-        starts = group['Start Pos'].tolist()
-        ends = group['End Pos'].tolist()
-        values = group['Prediction'].astype(float).tolist()
-        bw.addEntries(str(chr), starts, ends=ends, values=values)
+    # Create a list of tuples with (chromosome, start, end, value)
+    entries = [(row['Chr'], row['Start Pos'], row['End Pos'], row['Prediction'])
+               for _, row in df.iterrows()]
+
+    # Add entries to the BigWig file
+    bw.addEntries(entries)
 
     # Close the BigWig file
     bw.close()