fix

cas9on.py

@@ -208,9 +208,11 @@ def create_bigwig(df, bigwig_path):
     if not all(column in df.columns for column in ["Chr", "Start Pos", "End Pos", "Prediction"]):
         raise ValueError("DataFrame must contain 'Chr', 'Start Pos', 'End Pos', and 'Prediction' columns.")
 
-    # Prepare the BigWig header using unique chromosomes and their max position
+    # Convert positions to integers and prepare the BigWig header
+    df['Start Pos'] = df['Start Pos'].astype(int)
+    df['End Pos'] = df['End Pos'].astype(int)
     chr_sizes = df.groupby('Chr')['End Pos'].max().to_dict()
-    header = [(chr, size) for chr, size in chr_sizes.items()]
+    header = [(chr, int(size)) for chr, size in chr_sizes.items()]
 
     # Create a BigWig file
     bw = pyBigWig.open(bigwig_path, "w")
@@ -218,7 +220,7 @@ def create_bigwig(df, bigwig_path):
 
     # Add entries to the BigWig file
     for _, row in df.iterrows():
-        bw.addEntries([row['Chr']], [int(row['Start Pos'])], ends=[int(row['End Pos'])], values=[float(row['Prediction'])])
+        bw.addEntries([row['Chr']], [row['Start Pos']], ends=[row['End Pos']], values=[float(row['Prediction'])])
 
     # Close the BigWig file
-    bw.close()
+    bw.close()