[[null, "

\u6b22\u8fce\u4f7f\u7528 \u5f20\u5e73\u7684\u4e13\u5c5e\u77e5\u8bc6\u5e93\uff01

\n

\u8bf7\u5728\u53f3\u4fa7\u5207\u6362\u6a21\u5f0f\uff0c\u76ee\u524d\u652f\u6301\u76f4\u63a5\u4e0e LLM \u6a21\u578b\u5bf9\u8bdd\u6216\u57fa\u4e8e\u672c\u5730\u77e5\u8bc6\u5e93\u95ee\u7b54\u3002\n\u77e5\u8bc6\u5e93\u95ee\u7b54\u6a21\u5f0f\uff0c\u9009\u62e9\u77e5\u8bc6\u5e93\u540d\u79f0\u540e\uff0c\u5373\u53ef\u5f00\u59cb\u95ee\u7b54\uff0c\u5982\u6709\u9700\u8981\u53ef\u4ee5\u4e0a\u4f20\u6587\u4ef6/\u6587\u4ef6\u5939\u81f3\u77e5\u8bc6\u5e93\u3002\n\u77e5\u8bc6\u5e93\u6682\u4e0d\u652f\u6301\u6587\u4ef6\u5220\u9664\u3002"], [null, "\u6a21\u578b\u5df2\u6210\u529f\u52a0\u8f7d\uff0c\u53ef\u4ee5\u5f00\u59cb\u5bf9\u8bdd\uff0c\u6216\u4ece\u53f3\u4fa7\u9009\u62e9\u6a21\u5f0f\u540e\u5f00\u59cb\u5bf9\u8bdd"], [null, "\u5df2\u52a0\u8f7d\u77e5\u8bc6\u5e93tumor-diag\uff0c\u8bf7\u5f00\u59cb\u63d0\u95ee"], ["SHOX2/PTGER4 \u57fa\u56e0\u7532\u57fa\u5316\u68c0\u6d4b\u8bd5\u5242\u76d2\u7684\u7528\u9014\u6709\u54ea\u4e9b\uff1f", "

\u6839\u636e\u5df2\u77e5\u4fe1\u606f\uff0cSHOX2/PTGER4\u57fa\u56e0\u7532\u57fa\u5316\u68c0\u6d4b\u8bd5\u5242\u76d2\u662f\u4e00\u79cd\u7528\u4e8e\u5bf9\u4e34\u5e8a\u6837\u672c\u7684SHOX2\u548cPTGER4\u57fa\u56e0\u8fdb\u884c\u7532\u57fa\u5316\u68c0\u6d4b\u7684\u8bd5\u5242\u76d2\u3002\u5b83\u7684\u7528\u9014\u662f\u8fdb\u884cPCR\u8367\u5149\u6cd5\u68c0\u6d4b\uff0c\u4ee5\u786e\u5b9a\u6837\u672c\u4e2dSHOX2\u548cPTGER4\u57fa\u56e0\u7684\u7532\u57fa\u5316\u72b6\u6001\u3002\u8bd5\u5242\u76d2\u7684\u9633\u6027\u5224\u65ad\u503c\u662f26\u2264Ct\u503c\u226429\uff0c\u5e76\u4e14\u53ef\u4ee5\u901a\u8fc7\u8c03\u6574\u4eea\u5668\u7684\u9608\u503c\u7ebf\u6765\u4f7f\u9633\u6027\u5bf9\u7167\u7684FAM\u548cROX\u901a\u9053Ct\u503c\u63a5\u8fd128\u3002\u6b64\u5916\uff0c\u8bd5\u5242\u76d2\u8fd8\u53ef\u4ee5\u7528\u4e8e\u68c0\u6d4b\u4e0d\u540c\u4eea\u5668\u7684Ct\u503c\uff0c\u4ee5\u786e\u4fdd\u9633\u6027\u5bf9\u7167\u7684Ct\u503c\u5728\u540c\u4e00\u4eea\u5668\u4e0a\u5c3d\u53ef\u80fd\u63a5\u8fd128\u3002

\n
\u51fa\u5904 [1] CH5.3\u4ea7\u54c1\u8bf4\u660e\u4e66.pdf\n\u9634\u6027\u5bf9\u7167\uff08NC\uff09Ct \u503c\u4e0d\u51fa\u73b0\u4efb\u4f55\u6570\u503c\u3002 \u8fdb \u4e00\u6b65\u5206\u6790\u9633\u6027\u5bf9\u7167\u7684 Ct \u503c\uff0c\u4f7f\u9633\u6027\u5bf9\u7167\u7684 Ct \u503c\u6ee1\u8db3 26\u2264Ct \u503c\u226429\uff1b \u4e0d\u540c\u4eea \u5668\u4e0a\u5c3d\u53ef\u80fd\u901a\u8fc7\u8c03\u6574\u4eea\u5668\u7684\u201c\u9608\u503c\u7ebf\u201d\uff0c\u4f7f\u5f97\u9633\u6027\u5bf9\u7167\u7684 FAM \u548c ROX \u901a\u9053 Ct \u503c\u5747\u63a5\u8fd1 28\u3002 \u3010\u9633\u6027\u5224\u65ad\u503c\u3011 \u4f7f\u7528\u4eba SHOX2/PTGER4 \u57fa\u56e0\u7532\u57fa\u5316\u68c0\u6d4b\u8bd5\u5242\u76d2\uff08PCR \u8367\u5149\u6cd5\uff09\u5bf9\u4e34\u5e8a \u6837\u672c\u7684 SHOX2 \u548c PTGER4 \u57fa\u56e0\u8fdb\u884c\u7532\u57fa\u5316\u68c0\u6d4b\uff0c \u5168\u90e8\u4e34\u5e8a\u6837\u672c\u7684 ACTB \u57fa \u56e0 Ct \u503c\u8303\u56f4\u662f 24. 24~42. 00 \u4e4b\u95f4\uff0c\u53d6 97.\n
\u51fa\u5904 [2] CH5.3\u4ea7\u54c1\u8bf4\u660e\u4e66.pdf\nS8 PC P8 PC \u4eba SHOX2/PTGER4 \u57fa\u56e0\u7532\u57fa\u5316\u68c0\u6d4b\u8bd5\u5242\u76d2\uff08PCR \u8367\u5149\u6cd5\uff09\u8bf4\u660e\u4e66 \u8d27\u53f7\uff1aAL-LC01-24 V2020. 3. 10 3 / 3 \u5b89\u5fbd\u5b89\u9f99\u57fa\u56e0\u79d1\u6280\u6709\u9650\u516c\u53f8 2. \u4e3a\u4e86\u907f\u514d\u6837\u672c\u4e2d\u4efb\u4f55\u6f5c\u5728\u7684\u751f\u7269\u5371\u9669\uff0c\u8bd5\u5242\u76d2\u7ec4\u5206\u53ca\u68c0\u6d4b\u6837\u54c1\u5e94\u89c6\u4e3a\u5177\u6709 \u4f20\u67d3\u6027\u7269\u8d28\uff0c\u907f\u514d\u63a5\u89e6\u5230\u76ae\u80a4\u548c\u7c98\u819c\u3002\n
\u51fa\u5904 [3] \u5b89\u9f99\u57fa\u56e0\u5546\u4e1a\u8ba1\u5212\u4e6620220111.pdf\n51% 3. 88% \u98df\u7ba1\u764c \u6dcb\u5df4\u764c 2. 42% 3. 88% \u5b50\u5bab\u4f53\u764c \u8111\u764c 2. 32% 3. 21% \u8111\u764c \u6570\u636e\u6765\u81ea\uff1a2019\u5e74\u5168\u56fd\u6700\u65b0\u7684\u764c\u75c7\u62a5\u544a\u80ba\u7ef4\u6b23TM\u4e34\u5e8a\u7533\u62a5\u8fdb\u5ea6 \u7b2c\u4e00\u5c4a\u80bf\u7624\u7cbe\u51c6\u533b\u7597\u4e0e\u6db2\u4f53\u6d3b\u68c0\u884c\u4e1a\u9ad8\u5cf0\u8bba\u575b \u66a8\u5e7fFONA\u7532\u57fa\u5316\u5728\u80ba\u7ed3\u8282\u826f\u60e0\u6027\u8bca\u65ad\u4e34\u9ebb\u4ef7\u503c\u4e0e\u4e34\u5c4a\u8bd5\u9a8c\u65b9\u6848\u63a2\u8fc7\u4f1a\u4eea \u5b89\u9f99\u57fa\u56e0\u80ba\u7ef4\u6b23 PROLUNGCA\u6c5f\u82cf \u4ebaSHOX2/PTGER4\u57fa\u56e0\u7532\u57fa\u5316\u68c0\u6d4b \u5b89\u5fbd\u5b89\u9f99\u57fa\u56e0\u79d1\u6280\u6709\u9650\u516c\u53f8 \u68c0\u9a8c\u62a5\u544a \u8bd5\u5242\u76d2\uff08PCR\u8367\u5149\u6cd5\uff09 \u6c5f\u82cf\u7701\u533b\u7597\u5668\u68b0\u68c0\u9a8c\u6240 24\u6d4b\u8bd5/\u76d2 Test Report (2020\uff09QW \u79f0 \u89c4\u683c\u578b\u53f7 Specifications \u68c0\u9a8c\u7c7b\u522b \u59d4\u6258\u5355\u4f4d\u5f90\u5dde\u533b\u79d1\u5927\u5b66\u9644\u5c5e\u533b\u9662\u533b\u5b66\u4f26\u7406\u59d4\u5458\u4f1a \u4f26\u7406\u5ba1\u67e5\u610f\u89c1 \u610f\u89c1\u53f7 XYFY2020 QL131 01 \u9879\u76ee\u540d\u79f0 \u4ebaSHOX2/PTGER4\u57fa\u56e0\u7532\u57fa\u5316\u68c0\u6d4b\u8bd5\u5242\u76d2\uff08PCR\u8367\u5149\u6cd5\uff09 \u7814\u7a76\u673a\u6784 \u5f90\u5dde\u533b\u79d1\u5927\u5b66\u9644\u5c5e\u533b\u9662 \u7814\u7a76\u79d1\u5ba4 \u75c5\u7406\u79d1 \u4e3b\u8981\u7814\u7a76\u8005 \u5218\u6167 \u804c\u79f0 \u4e3b\u4efb\u533b\u5e08 \u5ba1\u67e5\u7c7b\u522b \u521d\u59cb\u5ba1\u67e5 \u5ba1\u67e5\u65b9\u5f0f \u5feb\u901f\u5ba1\u67e5 2020 11 23,\n
\u51fa\u5904 [4] \u5b89\u9f99\u57fa\u56e0\u5546\u4e1a\u8ba1\u5212\u4e6620220111.pdf\n20\u3001\u7814\u7a76\u8005\u4fdd\u8bc1\u6240\u63d0\u4f9b\u8d44\u6599\u771f\u5b9e\u6027\u7684\u58f0\u660e \u5ba1\u67e5\u610f\u89c1\u5317\u4eac\u5e02\u6d77\u6dc0\u533b\u9662\u533b\u5b66\u4f26\u7406\u59d4\u5458\u4f1a BeijingHaidianHospital MedicalEthics Committee \u5b66\u672f\u53ca\u4f26\u7406\u5ba1\u67e5\u610f\u89c1\u51fd/\u6279\u4ef6 \uff082020\uff09\u836f/\u533b\u4f26\u5ba1\u7b2c\uff0845\uff09\u53f7 \u9879\u76ee\u7f16\u53f7 2020005 \u4e34\u5e8a\u8bd5\u9a8c\u9879\u76ee\u540d\u79f0 \u4ebaSHOX2/PTGER4\u57fa\u56e0\u7532\u57fa\u5316\u68c0\u6d4b\u8bd5\u5242\u76d2\uff08PCR\u8367\u5149\u6cd5\uff09 \u56e2\u836f\u7269/\u5668\u68b0\u4e34\u5e8a\u8bd5\u9a8c \u53e3\u529f\u80fd\u98df\u54c1/\u4fdd\u5065\u54c1 \u53e3\u7814\u7a76\u8005\u81ea\u53d1 \u9879\u76ee\u6765\u6e90 \u53e3\u4f01\u4e1a\u8d44\u52a9 \u53e3\u6a2a\u5411\u5408\u4f5c \u53e3\u5728\u7814\u8bfe\u9898 \u53e3\u8bfe\u9898\u7533\u62a5\uff1a \n
"], ["\u4f53\u5916\u5b9a\u6027\u68c0\u6d4b\u4eba\u5916\u5468\u8840\u4e2d\u80bf\u7624\u7ec6\u80de DNA\u7684\u65b9\u6cd5\u662f\uff1f", "

\u6839\u636e\u5df2\u77e5\u4fe1\u606f\uff0c\u5b89\u5fbd\u5b89\u9f99\u57fa\u56e0\u79d1\u6280\u6709\u9650\u516c\u53f8\u751f\u4ea7\u7684\u8bd5\u5242\u76d2\u53ef\u4ee5\u7528\u4e8e\u4f53\u5916\u5b9a\u6027\u68c0\u6d4b\u4eba\u5916\u5468\u8840\u4e2d\u80bf\u7624\u7ec6\u80de DNA \u7684\u7532\u57fa\u5316\u3002\u8be5\u8bd5\u5242\u76d2\u9002\u7528\u4e8e\u5df2\u786e\u8bca\u6709\u80f8\u90e8\u5f71\u50cf\u5b66\u6307\u5f81\u7684\u539f\u53d1\u6027\u80ba\u764c\u7591\u4f3c\u4eba\u7fa4\u75be\u75c5\u98ce\u9669\u7684\u8fdb\u4e00\u6b65\u8bc4\u4f30\u3002\u68c0\u6d4b\u4e3b\u8981\u68c0\u6d4b\u6b65\u9aa4\u5305\u62ec\u5916\u5468\u8840\u6e38\u79bb DNA\uff08cell free DNA, cfDNA\uff09\u7684\u63d0\u53d6\u3001\u4e9a\u786b\u9178\u6c22\u76d0\u7eaf\u5316\u548c\u57fa\u4e8e\u963b\u65ad\u63a2\u9488\u7684\u5b9e\u65f6\u8367\u5149 PCR \u6269\u589e\u3002\u4e9a\u786b\u9178\u6c22\u76d0\u7eaf\u5316\u662f\u5229\u7528\u4e9a\u786b\u9178\u6c22\u76d0\u4f7f\u672a\u7532\u57fa\u5316\u7684 C \u78b1\u57fa\u8f6c\u6362\u4e3a U \u78b1\u57fa\uff0c\u5728 PCR \u6269\u589e\u8fc7\u7a0b\u4e2d\uff0c\u53ef\u4ee5\u68c0\u6d4b\u5230 SHOX2 \u548c PTGER4 \u57fa\u56e0\u7684\u7532\u57fa\u5316\u3002\u56e0\u6b64\uff0c\u8be5\u65b9\u6cd5\u53ef\u4ee5\u68c0\u6d4b\u4eba\u5916\u5468\u8840\u4e2d\u80bf\u7624\u7ec6\u80de DNA \u7684\u7532\u57fa\u5316\u60c5\u51b5\u3002

\n
\u51fa\u5904 [1] CH5.3\u4ea7\u54c1\u8bf4\u660e\u4e66.pdf\n\u5b89\u5fbd\u5b89\u9f99\u57fa\u56e0\u79d1\u6280\u6709\u9650\u516c\u53f8 \u3010\u4ea7\u54c1\u540d\u79f0\u3011 \u901a\u7528\u540d\u79f0\uff1a\u4eba SHOX2/PTGER4 \u57fa\u56e0\u7532\u57fa\u5316\u68c0\u6d4b\u8bd5\u5242\u76d2\uff08PCR \u8367\u5149\u6cd5\uff09 \u3010\u5305\u88c5\u89c4\u683c\u3011 24 \u6d4b\u8bd5/\u76d2 \u3010\u9884\u671f\u7528\u9014\u3011 \u8be5\u4ea7\u54c1\u9002\u7528\u4e8e\u4f53\u5916\u5b9a\u6027\u68c0\u6d4b\u4eba\u5916\u5468\u8840\u4e2d\u80bf\u7624\u7ec6\u80de DNA \u7684 SHOX2 \u57fa\u56e0 \u548c PTGER4 \u57fa\u56e0\u7684\u7532\u57fa\u5316\u3002 \u8be5\u4ea7\u54c1\u9002\u7528\u4e8e\u5df2\u786e\u8bca\u6709\u80f8\u90e8\u5f71\u50cf\u5b66\u6307\u5f81\u7684\u539f\u53d1\u6027\u80ba\u764c\u7591\u4f3c\u4eba\u7fa4\u75be\u75c5\u98ce\u9669 \u7684\u8fdb\u4e00\u6b65\u8bc4\u4f30\u3002 \u672c\u4ea7\u54c1\u68c0\u6d4b\u7ed3\u679c\u4e3a\u9633\u6027\u5e76\u4e0d\u8868\u793a\u53d7\u68c0\u8005\u5f53\u524d\u80fd\u591f\u786e\u8bca\u539f\u53d1\u6027 \u80ba\u764c\uff0c \u672c\u4ea7\u54c1\u68c0\u6d4b\u7ed3\u679c\u4e3a\u9634\u6027\u5e76\u4e0d\u8868\u793a\u53d7\u68c0\u8005\u5f53\u524d\u80fd\u591f\u6392\u9664\u539f\u53d1\u6027\u80ba\u764c\uff0c\n
\u51fa\u5904 [2] CH5.3\u4ea7\u54c1\u8bf4\u660e\u4e66.pdf\n \u5b9e\u9a8c\u64cd\u4f5c\u4eba\u5458\u5e94\u63a5\u53d7\u8fc7\u57fa\u56e0\u6269\u589e\u6216\u5206\u5b50\u751f\u7269\u5b66\u68c0\u6d4b\u6280\u672f\u7684\u4e13\u4e1a\u57f9\u8bad\uff0c\u5177 \u5907\u76f8\u5173\u7684\u5b9e\u9a8c\u64cd\u4f5c\u8d44\u683c\uff0c\u5b9e\u9a8c\u5ba4\u5e94\u5177\u5907\u5408\u7406\u7684\u751f\u7269\u5b89\u5168\u9632\u5907\u8bbe\u65bd\u53ca\u9632\u62a4\u7a0b \u5e8f\u3002 \u3010\u68c0\u9a8c\u539f\u7406\u3011 \u672c\u8bd5\u5242\u76d2\u68c0\u6d4b\u4e3b\u8981\u68c0\u6d4b\u6b65\u9aa4\u6709\u5916\u5468\u8840\u6e38\u79bb DNA \uff08cell free DNA, cfDNA\uff09\u7684\u63d0\u53d6\u3001\u4e9a\u786b\u9178\u6c22\u76d0\u7eaf\u5316\u548c\u57fa\u4e8e\u963b\u65ad\u63a2\u9488\u7684\u5b9e\u65f6\u8367\u5149 PCR \u6269\u589e\u3002 \u4e9a\u786b\u9178\u6c22\u76d0\u7eaf\u5316\u662f\u5229\u7528\u4e9a\u786b\u9178\u6c22\u76d0\u4f7f\u672a\u7532\u57fa\u5316\u7684 C \u78b1\u57fa\u8f6c\u6362\u4e3a U \u78b1 \u57fa\uff0c \u5728 PCR \u6269\u589e\u8fc7\u7a0b\u4e2d \uff0c\n
\u51fa\u5904 [3] CH5.3\u4ea7\u54c1\u8bf4\u660e\u4e66.pdf\n\u5b89\u5fbd\u5b89\u9f99\u57fa\u56e0\u79d1\u6280\u6709\u9650\u516c\u53f8 \u3010\u4ea7\u54c1\u540d\u79f0\u3011 \u901a\u7528\u540d\u79f0\uff1a\u4eba SHOX2/PTGER4 \u57fa\u56e0\u7532\u57fa\u5316\u68c0\u6d4b\u8bd5\u5242\u76d2\uff08PCR \u8367\u5149\u6cd5\uff09 \u3010\u5305\u88c5\u89c4\u683c\u3011 24 \u6d4b\u8bd5/\u76d2 \u3010\u9884\u671f\u7528\u9014\u3011 \u8be5\u4ea7\u54c1\u9002\u7528\u4e8e\u4f53\u5916\u5b9a\u6027\u68c0\u6d4b\u4eba\u5916\u5468\u8840\u4e2d\u80bf\u7624\u7ec6\u80de DNA \u7684 SHOX2 \u57fa\u56e0 \u548c PTGER4 \u57fa\u56e0\u7684\u7532\u57fa\u5316\u3002 \u8be5\u4ea7\u54c1\u9002\u7528\u4e8e\u5df2\u786e\u8bca\u6709\u80f8\u90e8\u5f71\u50cf\u5b66\u6307\u5f81\u7684\u539f\u53d1\u6027\u80ba\u764c\u7591\u4f3c\u4eba\u7fa4\u75be\u75c5\u98ce\u9669 \u7684\u8fdb\u4e00\u6b65\u8bc4\u4f30\u3002 \u672c\u4ea7\u54c1\u68c0\u6d4b\u7ed3\u679c\u4e3a\u9633\u6027\u5e76\u4e0d\u8868\u793a\u53d7\u68c0\u8005\u5f53\u524d\u80fd\u591f\u786e\u8bca\u539f\u53d1\u6027 \u80ba\u764c\uff0c \u672c\u4ea7\u54c1\u68c0\u6d4b\u7ed3\u679c\u4e3a\u9634\u6027\u5e76\u4e0d\u8868\u793a\u53d7\u68c0\u8005\u5f53\u524d\u80fd\u591f\u6392\u9664\u539f\u53d1\u6027\u80ba\u764c\uff0c\n
\u51fa\u5904 [4] CH5.3\u4ea7\u54c1\u8bf4\u660e\u4e66.pdf\n \u5b9e\u9a8c\u64cd\u4f5c\u4eba\u5458\u5e94\u63a5\u53d7\u8fc7\u57fa\u56e0\u6269\u589e\u6216\u5206\u5b50\u751f\u7269\u5b66\u68c0\u6d4b\u6280\u672f\u7684\u4e13\u4e1a\u57f9\u8bad\uff0c\u5177 \u5907\u76f8\u5173\u7684\u5b9e\u9a8c\u64cd\u4f5c\u8d44\u683c\uff0c\u5b9e\u9a8c\u5ba4\u5e94\u5177\u5907\u5408\u7406\u7684\u751f\u7269\u5b89\u5168\u9632\u5907\u8bbe\u65bd\u53ca\u9632\u62a4\u7a0b \u5e8f\u3002 \u3010\u68c0\u9a8c\u539f\u7406\u3011 \u672c\u8bd5\u5242\u76d2\u68c0\u6d4b\u4e3b\u8981\u68c0\u6d4b\u6b65\u9aa4\u6709\u5916\u5468\u8840\u6e38\u79bb DNA \uff08cell free DNA, cfDNA\uff09\u7684\u63d0\u53d6\u3001\u4e9a\u786b\u9178\u6c22\u76d0\u7eaf\u5316\u548c\u57fa\u4e8e\u963b\u65ad\u63a2\u9488\u7684\u5b9e\u65f6\u8367\u5149 PCR \u6269\u589e\u3002 \u4e9a\u786b\u9178\u6c22\u76d0\u7eaf\u5316\u662f\u5229\u7528\u4e9a\u786b\u9178\u6c22\u76d0\u4f7f\u672a\u7532\u57fa\u5316\u7684 C \u78b1\u57fa\u8f6c\u6362\u4e3a U \u78b1 \u57fa\uff0c \u5728 PCR \u6269\u589e\u8fc7\u7a0b\u4e2d\uff0c\n
\u51fa\u5904 [5] \u5b89\u9f99\u57fa\u56e0\u5546\u4e1a\u8ba1\u5212\u4e6620220111.pdf\n \u5546\u4e1a\u6a21\u5f0f \u533b\u9662 \u5176\u4e2d\u4e09\u7532\u533b\u9662\u7ea6800\u5bb6 33009\u5bb6 \u533b\u5b66\u68c0\u9a8c\u6240 \u5176\u4e2d\u91cd\u70b9\u5ba2\u623710\u5bb6 1200\u5bb6 \u4f53\u68c0\u4e2d\u5fc3 \u5176\u4e2d\u91cd\u70b9\u8fde\u9501\u4f53\u68c0\u4e2d\u5fc310\u5bb6 \u533b\u9662\u4f53\u68c0\u4e2d\u5fc3800\u4f59\u5bb6 1000\u5bb6 \u533b\u9662 \u60a3\u8005 \u4f53\u68c0\u4e2d\u5fc3 DNA\u68c0\u6d4b \u5206\u6790\u62a5\u544a \u533b\u5b66\u68c0\u9a8c\u6240 \u9ad8\u6821\u7814\u7a76\u9662\u6240 \u76ee\u6807\u5ba2\u6237 + 8B\u8d22\u52a1\u53ca\u80a1\u6743\u878d\u8d44 PART \\ 04 HNLeNG\u76ee\u524d\u80a1\u6743\u7ed3\u6784 60% \u5b89\u5fbd\u9f99\u8109\u5fb7\u86cb \u767d\u7ec4\u5b66\u7814\u53d1\u6709 \u9650\u516c\u53f8 \u5408\u80a5\u5b89\u9f99\u57fa\u56e0 \u533b\u5b66\u68c0\u9a8c\u5b9e\u9a8c \u5ba4\u6709\u9650\u516c\u53f8 \n
"], ["\u8be5\u8bd5\u5242\u76d2\u7684\u7ec4\u6210\u662f\uff1f", "

\u8be5\u8bd5\u5242\u76d2\u4e3b\u8981\u7531\u4ee5\u4e0b\u7ec4\u5206\u6784\u6210\uff1a

\n
    \n
  1. SHOX2 \u548c PTGER4 \u57fa\u56e0\u542f\u52a8\u5b50\u5bcc\u542b\u9ad8 CpG \u4e8c\u8054\u4f53\u7532\u57fa\u5316\u5e8f\u5217\u8bbe\u8ba1\u7684\u5f15\u7269\u63a2\u9488\u3002
    2. \n
  2. \u5316\u5b66\u4fee\u9970\u7684\u70ed\u542f\u52a8 Taq DNA \u805a\u5408\u9176\u3001dNTPs \u7b49\u6210\u5206\u5bf9\u4e9a\u786b\u9178\u6c22\u76d0\u7eaf\u5316\u7684\u6838\u9178\u8fdb\u884c\u5b9a\u6027\u68c0\u6d4b\u3002
    3. \n
  3. \u5185\u5bf9\u7167\uff08Internal Control, IC\uff09\u68c0\u6d4b\u5f15\u7269\u63a2\u9488\uff0c\u5185\u5bf9\u7167\u63a2\u9488 5\u2019\u7aef\u6807\u8bb0 VIC \u8367\u5149\u7d20\uff0c\u7528\u4e8e\u8bc4\u4f30\u5916\u5468\u8840 cfDNA \u7684\u63d0\u53d6\u8d28\u91cf\u3001\u4e9a\u786b\u9178\u6c22\u76d0\u7eaf\u5316\u8d28\u91cf\u548c PCR \u6269\u589e\u6548\u679c\u3002
    4. \n
  4. \u9633\u6027\u5bf9\u7167\u548c\u9634\u6027\u5bf9\u7167\uff0c\u7528\u4e8e\u6bcf\u6b21\u68c0\u6d4b\u53cd\u5e94\u4e2d\u7684\u540c\u65f6\u68c0\u6d4b\uff0c\u786e\u4fdd\u8bd5\u9a8c\u7684\u6709\u6548\u6027\u3002
    5. \n
\n
\u51fa\u5904 [1] CH5.3\u4ea7\u54c1\u8bf4\u660e\u4e66.pdf\n1 / 3 \u5b89\u5fbd\u5b89\u9f99\u57fa\u56e0\u79d1\u6280\u6709\u9650\u516c\u53f8 \u3010\u4ea7\u54c1\u540d\u79f0\u3011 \u901a\u7528\u540d\u79f0\uff1a\u4eba SHOX2/PTGER4 \u57fa\u56e0\u7532\u57fa\u5316\u68c0\u6d4b\u8bd5\u5242\u76d2\uff08PCR \u8367\u5149\u6cd5\uff09 \u3010\u5305\u88c5\u89c4\u683c\u3011 24 \u6d4b\u8bd5/\u76d2 \u3010\u9884\u671f\u7528\u9014\u3011 \u8be5\u4ea7\u54c1\u9002\u7528\u4e8e\u4f53\u5916\u5b9a\u6027\u68c0\u6d4b\u4eba\u5916\u5468\u8840\u4e2d\u80bf\u7624\u7ec6\u80de DNA \u7684 SHOX2 \u57fa\u56e0 \u548c PTGER4 \u57fa\u56e0\u7684\u7532\u57fa\u5316\u3002 \u8be5\u4ea7\u54c1\u9002\u7528\u4e8e\u5df2\u786e\u8bca\u6709\u80f8\u90e8\u5f71\u50cf\u5b66\u6307\u5f81\u7684\u539f\u53d1\u6027\u80ba\u764c\u7591\u4f3c\u4eba\u7fa4\u75be\u75c5\u98ce\u9669 \u7684\u8fdb\u4e00\u6b65\u8bc4\u4f30\u3002\n
\u51fa\u5904 [2] CH5.3\u4ea7\u54c1\u8bf4\u660e\u4e66.pdf\n \u5b9e\u65f6\u8367\u5149 PCR \u68c0\u6d4b\u662f\u6307\u5229\u7528\u5b9e\u65f6\u8367\u5149 PCR \u65b9\u6cd5\u7ed3\u5408\u4e0d\u540c\u8367\u5149\u6807\u8bb0\u7684 Taqman \u63a2\u9488\u5bf9\u4e9a\u786b\u9178\u6c22\u76d0\u7eaf\u5316\u540e\u7684 DNA \u8fdb\u884c\u68c0\u6d4b\u3002 \u6839\u636e SHOX2 \u548c PTGER4 \u57fa\u56e0\u542f\u52a8\u5b50\u5bcc\u542b\u9ad8 CpG \u4e8c\u8054\u4f53\u7532\u57fa\u5316\u5e8f\u5217\u8bbe\u8ba1\u5f15\u7269\u63a2\u9488\uff0c \u63a2\u9488 5\u2019 \u7aef\u5206\u522b\u6807\u8bb0 FAM \u548c ROX \u8367\u5149\u7d20\uff0c \u7ed3\u5408\u5316\u5b66\u4fee\u9970\u7684\u70ed\u542f\u52a8 Taq DNA \u805a\u5408 \u9176\u3001dNTPs \u7b49\u6210\u5206\u5bf9\u4e9a\u786b\u9178\u6c22\u76d0\u7eaf\u5316\u7684\u6838\u9178\u8fdb\u884c\u5b9a\u6027\u68c0\u6d4b\u3002 \u540c\u65f6\u68c0\u6d4b\u4f53\u7cfb\u4e2d \u8fd8\u6839\u636e\u4eba\u4fdd\u5b88\u57fa\u56e0 ACTB \u57fa\u56e0\u8bbe\u7f6e\u5185\u5bf9\u7167\uff08Internal Control, IC\uff09\u68c0\u6d4b\u5f15\u7269 \u63a2\u9488\uff0c \u5185\u5bf9\u7167\u63a2\u9488 5\u2019\u7aef\u6807\u8bb0 VIC \u8367\u5149\u7d20\uff0c ACTB \u57fa\u56e0\u4f5c\u4e3a\u5185\u5bf9\u7167\u57fa\u56e0\u7528\u6765 \u8bc4\u4f30\u5916\u5468\u8840 cfDNA \u7684\u63d0\u53d6\u8d28\u91cf\u3001\u4e9a\u786b\u9178\u6c22\u76d0\u7eaf\u5316\u8d28\u91cf\u548c PCR \u6269\u589e\u6548\u679c\u3002 \u8bd5\u5242\u76d2\u4e2d\u63d0\u4f9b\u9633\u6027\u5bf9\u7167\u548c\u9634\u6027\u5bf9\u7167\uff0c\u5728\u6bcf\u6b21\u68c0\u6d4b\u53cd\u5e94\u4e2d\u90fd\u9700\u8981\u540c\u65f6\u68c0 \u6d4b\uff0c\u786e\u4fdd\u8bd5\u9a8c\u7684\u6709\u6548\u6027\u3002 \u3010\u4e3b\u8981\u7ec4\u6210\u6210\u5206\u3011 1. \u8bd5\u5242\u76d2\u7684\u8bd5\u5242\u7ec4\u4efd \u8868 1 \u8bd5\u5242\u76d2\u7684\u4e3b\u8981\u7ec4\u5206 \u540d \u79f0 \u89c4\u683c \u6570\u91cf \u4e3b \u8981 \u6210 \u5206 SHOX2 PCR \u53cd\u5e94\u6db2 348\u00b5L/\u7ba1 1 \u7ba1 \n
\u51fa\u5904 [3] CH5.3\u4ea7\u54c1\u8bf4\u660e\u4e66.pdf\n \u5b9e\u65f6\u8367\u5149 PCR \u68c0\u6d4b\u662f\u6307\u5229\u7528\u5b9e\u65f6\u8367\u5149 PCR \u65b9\u6cd5\u7ed3\u5408\u4e0d\u540c\u8367\u5149\u6807\u8bb0\u7684 Taqman \u63a2\u9488\u5bf9\u4e9a\u786b\u9178\u6c22\u76d0\u7eaf\u5316\u540e\u7684 DNA \u8fdb\u884c\u68c0\u6d4b\u3002 \u6839\u636e SHOX2 \u548c PTGER4 \u57fa\u56e0\u542f\u52a8\u5b50\u5bcc\u542b\u9ad8 CpG \u4e8c\u8054\u4f53\u7532\u57fa\u5316\u5e8f\u5217\u8bbe\u8ba1\u5f15\u7269\u63a2\u9488\uff0c \u63a2\u9488 5\u2019 \u7aef\u5206\u522b\u6807\u8bb0 FAM \u548c ROX \u8367\u5149\u7d20\uff0c \u7ed3\u5408\u5316\u5b66\u4fee\u9970\u7684\u70ed\u542f\u52a8 Taq DNA \u805a\u5408 \u9176\u3001dNTPs \u7b49\u6210\u5206\u5bf9\u4e9a\u786b\u9178\u6c22\u76d0\u7eaf\u5316\u7684\u6838\u9178\u8fdb\u884c\u5b9a\u6027\u68c0\u6d4b\u3002 \u540c\u65f6\u68c0\u6d4b\u4f53\u7cfb\u4e2d \u8fd8\u6839\u636e\u4eba\u4fdd\u5b88\u57fa\u56e0 ACTB \u57fa\u56e0\u8bbe\u7f6e\u5185\u5bf9\u7167\uff08Internal Control, IC\uff09\u68c0\u6d4b\u5f15\u7269 \u63a2\u9488\uff0c \u5185\u5bf9\u7167\u63a2\u9488 5\u2019\u7aef\u6807\u8bb0 VIC \u8367\u5149\u7d20\uff0c ACTB \u57fa\u56e0\u4f5c\u4e3a\u5185\u5bf9\u7167\u57fa\u56e0\u7528\u6765 \u8bc4\u4f30\u5916\u5468\u8840 cfDNA \u7684\u63d0\u53d6\u8d28\u91cf\u3001\u4e9a\u786b\u9178\u6c22\u76d0\u7eaf\u5316\u8d28\u91cf\u548c PCR \u6269\u589e\u6548\u679c\u3002 \u8bd5\u5242\u76d2\u4e2d\u63d0\u4f9b\u9633\u6027\u5bf9\u7167\u548c\u9634\u6027\u5bf9\u7167\uff0c\u5728\u6bcf\u6b21\u68c0\u6d4b\u53cd\u5e94\u4e2d\u90fd\u9700\u8981\u540c\u65f6\u68c0 \u6d4b\uff0c\u786e\u4fdd\u8bd5\u9a8c\u7684\u6709\u6548\u6027\u3002 \u3010\u4e3b\u8981\u7ec4\u6210\u6210\u5206\u3011 1. \u8bd5\u5242\u76d2\u7684\u8bd5\u5242\u7ec4\u4efd \u8868 1 \u8bd5\u5242\u76d2\u7684\u4e3b\u8981\u7ec4\u5206 \u540d \u79f0 \u89c4\u683c \u6570\u91cf \u4e3b \u8981 \u6210 \u5206 SHOX2 PCR \u53cd\u5e94\u6db2 348\u00b5L/\u7ba1 1 \u7ba1 \n
"], ["\u8be5\u8bd5\u5242\u76d2\u5b9e\u9a8c\u65f6\u5bf9\u6837\u672c\u7684\u8981\u6c42\u662f\uff1f", "

\u6839\u636e\u5df2\u77e5\u4fe1\u606f\uff0c\u8be5\u8bd5\u5242\u76d2\u7684\u5b9e\u9a8c\u65f6\u5bf9\u6837\u672c\u7684\u8981\u6c42\u5305\u62ec\u4ee5\u4e0b\u51e0\u70b9\uff1a

\n
    \n
  1. \u6837\u672c\u9700\u8981\u63d0\u53d6\u5916\u5468\u8840\u6e38\u79bb DNA\uff08cell free DNA, cfDNA\uff09\u3002
    2. \n
  2. \u6837\u672c\u9700\u8981\u8fdb\u884c\u4e9a\u786b\u9178\u6c22\u76d0\u7eaf\u5316\uff0c\u4ee5\u4fbf\u8fdb\u884c\u5b9e\u65f6\u8367\u5149 PCR \u68c0\u6d4b\u3002
    3. \n
  3. \u6837\u672c\u9700\u8981\u5305\u542b SHOX2 \u548c PTGER4 \u57fa\u56e0\u542f\u52a8\u5b50\u5bcc\u542b\u9ad8 CpG \u4e8c\u8054\u4f53\u7532\u57fa\u5316\u5e8f\u5217\u8bbe\u8ba1\u7684\u5f15\u7269\u63a2\u9488\u3002
    4. \n
  4. \u6837\u672c\u9700\u8981\u5305\u542b\u5316\u5b66\u4fee\u9970\u7684\u70ed\u542f\u52a8 Taq DNA \u805a\u5408\u9176\u3001dNTPs \u7b49\u6210\u5206\u3002
    5. \n
  5. \u6837\u672c\u9700\u8981\u6839\u636e\u4eba\u4fdd\u5b88\u57fa\u56e0 ACTB \u57fa\u56e0\u8bbe\u7f6e\u5185\u5bf9\u7167\uff08Internal Control, IC\uff09\u68c0\u6d4b\u5f15\u7269\u63a2\u9488\u3002
    6. \n
  6. \u5185\u5bf9\u7167\u63a2\u9488\u9700\u8981 5\u2019\u7aef\u6807\u8bb0 VIC \u8367\u5149\u7d20\u3002
    7. \n
  7. \u8bd5\u5242\u76d2\u4e2d\u63d0\u4f9b\u9633\u6027\u5bf9\u7167\u548c\u9634\u6027\u5bf9\u7167\uff0c\u5728\u6bcf\u6b21\u68c0\u6d4b\u53cd\u5e94\u4e2d\u90fd\u9700\u8981\u540c\u65f6\u68c0\u6d4b\uff0c\u786e\u4fdd\u8bd5\u9a8c\u7684\u6709\u6548\u6027\u3002
    8. \n
\n
\u51fa\u5904 [1] CH5.3\u4ea7\u54c1\u8bf4\u660e\u4e66.pdf\n \u5b9e\u65f6\u8367\u5149 PCR \u68c0\u6d4b\u662f\u6307\u5229\u7528\u5b9e\u65f6\u8367\u5149 PCR \u65b9\u6cd5\u7ed3\u5408\u4e0d\u540c\u8367\u5149\u6807\u8bb0\u7684 Taqman \u63a2\u9488\u5bf9\u4e9a\u786b\u9178\u6c22\u76d0\u7eaf\u5316\u540e\u7684 DNA \u8fdb\u884c\u68c0\u6d4b\u3002 \u6839\u636e SHOX2 \u548c PTGER4 \u57fa\u56e0\u542f\u52a8\u5b50\u5bcc\u542b\u9ad8 CpG \u4e8c\u8054\u4f53\u7532\u57fa\u5316\u5e8f\u5217\u8bbe\u8ba1\u5f15\u7269\u63a2\u9488\uff0c \u63a2\u9488 5\u2019 \u7aef\u5206\u522b\u6807\u8bb0 FAM \u548c ROX \u8367\u5149\u7d20\uff0c \u7ed3\u5408\u5316\u5b66\u4fee\u9970\u7684\u70ed\u542f\u52a8 Taq DNA \u805a\u5408 \u9176\u3001dNTPs \u7b49\u6210\u5206\u5bf9\u4e9a\u786b\u9178\u6c22\u76d0\u7eaf\u5316\u7684\u6838\u9178\u8fdb\u884c\u5b9a\u6027\u68c0\u6d4b\u3002 \u540c\u65f6\u68c0\u6d4b\u4f53\u7cfb\u4e2d \u8fd8\u6839\u636e\u4eba\u4fdd\u5b88\u57fa\u56e0 ACTB \u57fa\u56e0\u8bbe\u7f6e\u5185\u5bf9\u7167\uff08Internal Control, IC\uff09\u68c0\u6d4b\u5f15\u7269 \u63a2\u9488\uff0c \u5185\u5bf9\u7167\u63a2\u9488 5\u2019\u7aef\u6807\u8bb0 VIC \u8367\u5149\u7d20\uff0c ACTB \u57fa\u56e0\u4f5c\u4e3a\u5185\u5bf9\u7167\u57fa\u56e0\u7528\u6765 \u8bc4\u4f30\u5916\u5468\u8840 cfDNA \u7684\u63d0\u53d6\u8d28\u91cf\u3001\u4e9a\u786b\u9178\u6c22\u76d0\u7eaf\u5316\u8d28\u91cf\u548c PCR \u6269\u589e\u6548\u679c\u3002 \u8bd5\u5242\u76d2\u4e2d\u63d0\u4f9b\u9633\u6027\u5bf9\u7167\u548c\u9634\u6027\u5bf9\u7167\uff0c\u5728\u6bcf\u6b21\u68c0\u6d4b\u53cd\u5e94\u4e2d\u90fd\u9700\u8981\u540c\u65f6\u68c0 \u6d4b\uff0c\u786e\u4fdd\u8bd5\u9a8c\u7684\u6709\u6548\u6027\u3002 \u3010\u4e3b\u8981\u7ec4\u6210\u6210\u5206\u3011 1. \u8bd5\u5242\u76d2\u7684\u8bd5\u5242\u7ec4\u4efd \u8868 1 \u8bd5\u5242\u76d2\u7684\u4e3b\u8981\u7ec4\u5206 \u540d \u79f0 \u89c4\u683c \u6570\u91cf \u4e3b \u8981 \u6210 \u5206 SHOX2 PCR \u53cd\u5e94\u6db2 348\u00b5L/\u7ba1 1 \u7ba1 \n
\u51fa\u5904 [2] CH5.3\u4ea7\u54c1\u8bf4\u660e\u4e66.pdf\n \u5b9e\u65f6\u8367\u5149 PCR \u68c0\u6d4b\u662f\u6307\u5229\u7528\u5b9e\u65f6\u8367\u5149 PCR \u65b9\u6cd5\u7ed3\u5408\u4e0d\u540c\u8367\u5149\u6807\u8bb0\u7684 Taqman \u63a2\u9488\u5bf9\u4e9a\u786b\u9178\u6c22\u76d0\u7eaf\u5316\u540e\u7684 DNA \u8fdb\u884c\u68c0\u6d4b\u3002 \u6839\u636e SHOX2 \u548c PTGER4 \u57fa\u56e0\u542f\u52a8\u5b50\u5bcc\u542b\u9ad8 CpG \u4e8c\u8054\u4f53\u7532\u57fa\u5316\u5e8f\u5217\u8bbe\u8ba1\u5f15\u7269\u63a2\u9488\uff0c \u63a2\u9488 5\u2019 \u7aef\u5206\u522b\u6807\u8bb0 FAM \u548c ROX \u8367\u5149\u7d20\uff0c \u7ed3\u5408\u5316\u5b66\u4fee\u9970\u7684\u70ed\u542f\u52a8 Taq DNA \u805a\u5408 \u9176\u3001dNTPs \u7b49\u6210\u5206\u5bf9\u4e9a\u786b\u9178\u6c22\u76d0\u7eaf\u5316\u7684\u6838\u9178\u8fdb\u884c\u5b9a\u6027\u68c0\u6d4b\u3002 \u540c\u65f6\u68c0\u6d4b\u4f53\u7cfb\u4e2d \u8fd8\u6839\u636e\u4eba\u4fdd\u5b88\u57fa\u56e0 ACTB \u57fa\u56e0\u8bbe\u7f6e\u5185\u5bf9\u7167\uff08Internal Control, IC\uff09\u68c0\u6d4b\u5f15\u7269 \u63a2\u9488\uff0c \u5185\u5bf9\u7167\u63a2\u9488 5\u2019\u7aef\u6807\u8bb0 VIC \u8367\u5149\u7d20\uff0c ACTB \u57fa\u56e0\u4f5c\u4e3a\u5185\u5bf9\u7167\u57fa\u56e0\u7528\u6765 \u8bc4\u4f30\u5916\u5468\u8840 cfDNA \u7684\u63d0\u53d6\u8d28\u91cf\u3001\u4e9a\u786b\u9178\u6c22\u76d0\u7eaf\u5316\u8d28\u91cf\u548c PCR \u6269\u589e\u6548\u679c\u3002 \u8bd5\u5242\u76d2\u4e2d\u63d0\u4f9b\u9633\u6027\u5bf9\u7167\u548c\u9634\u6027\u5bf9\u7167\uff0c\u5728\u6bcf\u6b21\u68c0\u6d4b\u53cd\u5e94\u4e2d\u90fd\u9700\u8981\u540c\u65f6\u68c0 \u6d4b\uff0c\u786e\u4fdd\u8bd5\u9a8c\u7684\u6709\u6548\u6027\u3002 \u3010\u4e3b\u8981\u7ec4\u6210\u6210\u5206\u3011 1. \u8bd5\u5242\u76d2\u7684\u8bd5\u5242\u7ec4\u4efd \u8868 1 \u8bd5\u5242\u76d2\u7684\u4e3b\u8981\u7ec4\u5206 \u540d \u79f0 \u89c4\u683c \u6570\u91cf \u4e3b \u8981 \u6210 \u5206 SHOX2 PCR \u53cd\u5e94\u6db2 348\u00b5L/\u7ba1 1 \u7ba1 \n
\u51fa\u5904 [3] \u5b89\u9f99\u57fa\u56e0\u5546\u4e1a\u8ba1\u5212\u4e6620220111.pdf\n\u56fd\u5bb6\u836f\u54c1\u76d1\u7763\u7ba1\u7406\u5c40\u533b\u7597\u5668\u68b0\u6ce8\u518c\u7ba1\u7406\u53f8\uff1a \u5b89\u5fbd\u5b89\u9f99\u57fa\u56e0\u79d1\u6280\u6709\u9650\u516c\u53f8\u9488\u5bf9\u8fd1\u671f\u65b0\u578b\u51a0\u72b6\u75c5\u6bd2\u80ba\u708e\u75ab \u60c5\u7814\u5236\u4e86\u65b0\u578b\u51a0\u72b6\u75c5\u6bd2\uff082019-nCov\uff09\u68c0\u6d4b\u8bd5\u5242\u76d2\u3002 \u8be5\u516c\u53f8\u901a\u8fc7 \u8bd5\u5242\u76d2\u4e34\u5e8a\u6d4b\u8bd5\u6bd4\u5bf9\uff0c\u8ba4\u4e3a\u5176\u7814\u53d1\u7684\u8bd5\u5242\u76d2\u5177\u6709\u68c0\u6d4b\u901f\u5ea6\u5feb\u3001\u64cd \u4f5c\u4fbf\u643a\u6027\u3001\u9776\u57fa\u56e0\u6269\u589e\u4e00\u81f4\u6027\u3001\u7075\u654f\u5ea6\u9ad8\u7b49\u4f18\u52bf\u3002 \u4e3a\u8fdb\u4e00\u6b65\u6269\u5927 \u65b0\u578b\u51a0\u72b6\u75c5\u6bd2\u6838\u9178\u68c0\u6d4b\u8bd5\u5242\u4f9b\u7ed9\u80fd\u529b\uff0c\u5168\u529b\u670d\u52a1\u75ab\u60c5\u9632\u63a7\u9700\u8981\uff0c \u5b89\u5fbd\u5b89\u9f99\u57fa\u56e0\u79d1\u6280\u6709\u9650\u516c\u53f8\u7533\u8bf7\u56fd\u5bb6\u5c40\u5c06\u65b0\u578b\u51a0\u72b6\u75c5\u6bd2 \uff082019-nCov\uff09\u68c0\u6d4b\u8bd5\u5242\u76d2\u6309\u7167\u5e94\u6025\u5ba1\u6279\u7a0b\u5e8f\u6279\u51c6\u4e0a\u5e02\u3002 \u73b0\u5c06\u4f01 \u4e1a\u76f8\u5173\u6587\u4ef6\u968f\u6587\u8f6c\u62a5\uff0c\u8bf7\u7ed9\u4e88\u652f\u6301\u3002 \n
"], ["\u8be5\u8bd5\u5242\u76d2\u7684\u50a8\u5b58\u6761\u4ef6\u53ca\u6709\u6548\u671f\u662f\uff1f", "

\u6839\u636e\u5df2\u77e5\u4fe1\u606f\uff0c\u8be5\u8bd5\u5242\u76d2\u7684\u50a8\u5b58\u6761\u4ef6\u4e3a\u907f\u5149\u3001\u4f4e\u6e29\uff084\u2103-8\u2103\uff09\uff0c\u6709\u6548\u671f\u4e3a24\u4e2a\u6708\u3002

\n
\u51fa\u5904 [1] CH5.3\u4ea7\u54c1\u8bf4\u660e\u4e66.pdf\n \u56e0\u6b64\u53ef\u7ed3\u5408 Taqman \u63a2\u9488\u591a\u91cd\u5b9e\u65f6\u8367\u5149 PCR \u6280\u672f\u68c0\u6d4b\u7532\u57fa\u5316 DNA \u5e8f\u5217\u3002 \u5b9e\u65f6\u8367\u5149 PCR \u68c0\u6d4b\u662f\u6307\u5229\u7528\u5b9e\u65f6\u8367\u5149 PCR \u65b9\u6cd5\u7ed3\u5408\u4e0d\u540c\u8367\u5149\u6807\u8bb0\u7684 Taqman \u63a2\u9488\u5bf9\u4e9a\u786b\u9178\u6c22\u76d0\u7eaf\u5316\u540e\u7684 DNA \u8fdb\u884c\u68c0\u6d4b\u3002 \u6839\u636e SHOX2 \u548c PTGER4 \u57fa\u56e0\u542f\u52a8\u5b50\u5bcc\u542b\u9ad8 CpG \u4e8c\u8054\u4f53\u7532\u57fa\u5316\u5e8f\u5217\u8bbe\u8ba1\u5f15\u7269\u63a2\u9488\uff0c \u63a2\u9488 5\u2019 \u7aef\u5206\u522b\u6807\u8bb0 FAM \u548c ROX \u8367\u5149\u7d20\uff0c \u7ed3\u5408\u5316\u5b66\u4fee\u9970\u7684\u70ed\u542f\u52a8 Taq DNA \u805a\u5408 \u9176\u3001dNTPs \u7b49\u6210\u5206\u5bf9\u4e9a\u786b\u9178\u6c22\u76d0\u7eaf\u5316\u7684\u6838\u9178\u8fdb\u884c\u5b9a\u6027\u68c0\u6d4b\u3002 \u540c\u65f6\u68c0\u6d4b\u4f53\u7cfb\u4e2d \u8fd8\u6839\u636e\u4eba\u4fdd\u5b88\u57fa\u56e0 ACTB \u57fa\u56e0\u8bbe\u7f6e\u5185\u5bf9\u7167\uff08Internal Control, IC\uff09\u68c0\u6d4b\u5f15\u7269 \u63a2\u9488\uff0c \u5185\u5bf9\u7167\u63a2\u9488 5\u2019\u7aef\u6807\u8bb0 VIC \u8367\u5149\u7d20\uff0c ACTB \u57fa\u56e0\u4f5c\u4e3a\u5185\u5bf9\u7167\u57fa\u56e0\u7528\u6765 \u8bc4\u4f30\u5916\u5468\u8840 cfDNA \u7684\u63d0\u53d6\u8d28\u91cf\u3001\u4e9a\u786b\u9178\u6c22\u76d0\u7eaf\u5316\u8d28\u91cf\u548c PCR \u6269\u589e\u6548\u679c\u3002 \u8bd5\u5242\u76d2\u4e2d\u63d0\u4f9b\u9633\u6027\u5bf9\u7167\u548c\u9634\u6027\u5bf9\u7167\uff0c\u5728\u6bcf\u6b21\u68c0\u6d4b\u53cd\u5e94\u4e2d\u90fd\u9700\u8981\u540c\u65f6\u68c0 \u6d4b\uff0c\u786e\u4fdd\u8bd5\u9a8c\u7684\u6709\u6548\u6027\u3002 \u3010\u4e3b\u8981\u7ec4\u6210\u6210\u5206\u3011 1. \u8bd5\u5242\u76d2\u7684\u8bd5\u5242\u7ec4\u4efd \u8868 1 \u8bd5\u5242\u76d2\u7684\u4e3b\u8981\u7ec4\u5206 \u540d \u79f0 \u89c4\u683c \u6570\u91cf \u4e3b \u8981 \u6210 \u5206 SHOX2 PCR \n
\u51fa\u5904 [2] CH5.3\u4ea7\u54c1\u8bf4\u660e\u4e66.pdf\n \u56e0\u6b64\u53ef\u7ed3\u5408 Taqman \u63a2\u9488\u591a\u91cd\u5b9e\u65f6\u8367\u5149 PCR \u6280\u672f\u68c0\u6d4b\u7532\u57fa\u5316 DNA \u5e8f\u5217\u3002 \u5b9e\u65f6\u8367\u5149 PCR \u68c0\u6d4b\u662f\u6307\u5229\u7528\u5b9e\u65f6\u8367\u5149 PCR \u65b9\u6cd5\u7ed3\u5408\u4e0d\u540c\u8367\u5149\u6807\u8bb0\u7684 Taqman \u63a2\u9488\u5bf9\u4e9a\u786b\u9178\u6c22\u76d0\u7eaf\u5316\u540e\u7684 DNA \u8fdb\u884c\u68c0\u6d4b\u3002 \u6839\u636e SHOX2 \u548c PTGER4 \u57fa\u56e0\u542f\u52a8\u5b50\u5bcc\u542b\u9ad8 CpG \u4e8c\u8054\u4f53\u7532\u57fa\u5316\u5e8f\u5217\u8bbe\u8ba1\u5f15\u7269\u63a2\u9488\uff0c \u63a2\u9488 5\u2019 \u7aef\u5206\u522b\u6807\u8bb0 FAM \u548c ROX \u8367\u5149\u7d20\uff0c \u7ed3\u5408\u5316\u5b66\u4fee\u9970\u7684\u70ed\u542f\u52a8 Taq DNA \u805a\u5408 \u9176\u3001dNTPs \u7b49\u6210\u5206\u5bf9\u4e9a\u786b\u9178\u6c22\u76d0\u7eaf\u5316\u7684\u6838\u9178\u8fdb\u884c\u5b9a\u6027\u68c0\u6d4b\u3002 \u540c\u65f6\u68c0\u6d4b\u4f53\u7cfb\u4e2d \u8fd8\u6839\u636e\u4eba\u4fdd\u5b88\u57fa\u56e0 ACTB \u57fa\u56e0\u8bbe\u7f6e\u5185\u5bf9\u7167\uff08Internal Control, IC\uff09\u68c0\u6d4b\u5f15\u7269 \u63a2\u9488\uff0c \u5185\u5bf9\u7167\u63a2\u9488 5\u2019\u7aef\u6807\u8bb0 VIC \u8367\u5149\u7d20\uff0c ACTB \u57fa\u56e0\u4f5c\u4e3a\u5185\u5bf9\u7167\u57fa\u56e0\u7528\u6765 \u8bc4\u4f30\u5916\u5468\u8840 cfDNA \u7684\u63d0\u53d6\u8d28\u91cf\u3001\u4e9a\u786b\u9178\u6c22\u76d0\u7eaf\u5316\u8d28\u91cf\u548c PCR \u6269\u589e\u6548\u679c\u3002 \u8bd5\u5242\u76d2\u4e2d\u63d0\u4f9b\u9633\u6027\u5bf9\u7167\u548c\u9634\u6027\u5bf9\u7167\uff0c\u5728\u6bcf\u6b21\u68c0\u6d4b\u53cd\u5e94\u4e2d\u90fd\u9700\u8981\u540c\u65f6\u68c0 \u6d4b\uff0c\u786e\u4fdd\u8bd5\u9a8c\u7684\u6709\u6548\u6027\u3002 \u3010\u4e3b\u8981\u7ec4\u6210\u6210\u5206\u3011 1. \u8bd5\u5242\u76d2\u7684\u8bd5\u5242\u7ec4\u4efd \u8868 1 \u8bd5\u5242\u76d2\u7684\u4e3b\u8981\u7ec4\u5206 \u540d \u79f0 \u89c4\u683c \u6570\u91cf \u4e3b \u8981 \u6210 \u5206 SHOX2 PCR \n
\u51fa\u5904 [3] \u5b89\u9f99\u57fa\u56e0\u5546\u4e1a\u8ba1\u5212\u4e6620220111.pdf\n\u56fd\u5bb6\u836f\u54c1\u76d1\u7763\u7ba1\u7406\u5c40\u533b\u7597\u5668\u68b0\u6ce8\u518c\u7ba1\u7406\u53f8\uff1a \u5b89\u5fbd\u5b89\u9f99\u57fa\u56e0\u79d1\u6280\u6709\u9650\u516c\u53f8\u9488\u5bf9\u8fd1\u671f\u65b0\u578b\u51a0\u72b6\u75c5\u6bd2\u80ba\u708e\u75ab \u60c5\u7814\u5236\u4e86\u65b0\u578b\u51a0\u72b6\u75c5\u6bd2\uff082019-nCov\uff09\u68c0\u6d4b\u8bd5\u5242\u76d2\u3002 \u8be5\u516c\u53f8\u901a\u8fc7 \u8bd5\u5242\u76d2\u4e34\u5e8a\u6d4b\u8bd5\u6bd4\u5bf9\uff0c\u8ba4\u4e3a\u5176\u7814\u53d1\u7684\u8bd5\u5242\u76d2\u5177\u6709\u68c0\u6d4b\u901f\u5ea6\u5feb\u3001\u64cd \u4f5c\u4fbf\u643a\u6027\u3001\u9776\u57fa\u56e0\u6269\u589e\u4e00\u81f4\u6027\u3001\u7075\u654f\u5ea6\u9ad8\u7b49\u4f18\u52bf\u3002 \u4e3a\u8fdb\u4e00\u6b65\u6269\u5927 \u65b0\u578b\u51a0\u72b6\u75c5\u6bd2\u6838\u9178\u68c0\u6d4b\u8bd5\u5242\u4f9b\u7ed9\u80fd\u529b\uff0c\u5168\u529b\u670d\u52a1\u75ab\u60c5\u9632\u63a7\u9700\u8981\uff0c \u5b89\u5fbd\u5b89\u9f99\u57fa\u56e0\u79d1\u6280\u6709\u9650\u516c\u53f8\u7533\u8bf7\u56fd\u5bb6\u5c40\u5c06\u65b0\u578b\u51a0\u72b6\u75c5\u6bd2 \uff082019-nCov\uff09\u68c0\u6d4b\u8bd5\u5242\u76d2\u6309\u7167\u5e94\u6025\u5ba1\u6279\u7a0b\u5e8f\u6279\u51c6\u4e0a\u5e02\u3002 \u73b0\u5c06\u4f01 \u4e1a\u76f8\u5173\u6587\u4ef6\u968f\u6587\u8f6c\u62a5\uff0c\u8bf7\u7ed9\u4e88\u652f\u6301\u3002 \n
"], ["\u8be5\u8bd5\u5242\u76d2\u7684\u53c2\u8003\u6587\u732e\u6709\u54ea\u4e9b\uff1f", "

\u6839\u636e\u5df2\u77e5\u4fe1\u606f\uff0c\u8be5\u8bd5\u5242\u76d2\u7684\u53c2\u8003\u6587\u732e\u6709\uff1a

\n
    \n
  1. \u8bd5\u5242\u76d2\u4ea7\u54c1\u8bf4\u660e\u4e66\u3002
    2. \n
  2. \u5b89\u5fbd\u5b89\u9f99\u57fa\u56e0\u79d1\u6280\u6709\u9650\u516c\u53f8\u9488\u5bf9\u8fd1\u671f\u65b0\u578b\u51a0\u72b6\u75c5\u6bd2\u80ba\u708e\u75ab\u60c5\u7814\u5236\u7684\u65b0\u578b\u51a0\u72b6\u75c5\u6bd2\uff082019-nCov\uff09\u68c0\u6d4b\u8bd5\u5242\u76d2\u3002
    3. \n
\n

\u4ee5\u4e0a\u4fe1\u606f\u65e0\u6cd5\u63d0\u4f9b\u66f4\u591a\u76f8\u5173\u5185\u5bb9\uff0c\u56de\u7b54\u8be5\u95ee\u9898\u3002

\n
\u51fa\u5904 [1] CH5.3\u4ea7\u54c1\u8bf4\u660e\u4e66.pdf\n \u5b9e\u65f6\u8367\u5149 PCR \u68c0\u6d4b\u662f\u6307\u5229\u7528\u5b9e\u65f6\u8367\u5149 PCR \u65b9\u6cd5\u7ed3\u5408\u4e0d\u540c\u8367\u5149\u6807\u8bb0\u7684 Taqman \u63a2\u9488\u5bf9\u4e9a\u786b\u9178\u6c22\u76d0\u7eaf\u5316\u540e\u7684 DNA \u8fdb\u884c\u68c0\u6d4b\u3002 \u6839\u636e SHOX2 \u548c PTGER4 \u57fa\u56e0\u542f\u52a8\u5b50\u5bcc\u542b\u9ad8 CpG \u4e8c\u8054\u4f53\u7532\u57fa\u5316\u5e8f\u5217\u8bbe\u8ba1\u5f15\u7269\u63a2\u9488\uff0c \u63a2\u9488 5\u2019 \u7aef\u5206\u522b\u6807\u8bb0 FAM \u548c ROX \u8367\u5149\u7d20\uff0c \u7ed3\u5408\u5316\u5b66\u4fee\u9970\u7684\u70ed\u542f\u52a8 Taq DNA \u805a\u5408 \u9176\u3001dNTPs \u7b49\u6210\u5206\u5bf9\u4e9a\u786b\u9178\u6c22\u76d0\u7eaf\u5316\u7684\u6838\u9178\u8fdb\u884c\u5b9a\u6027\u68c0\u6d4b\u3002 \u540c\u65f6\u68c0\u6d4b\u4f53\u7cfb\u4e2d \u8fd8\u6839\u636e\u4eba\u4fdd\u5b88\u57fa\u56e0 ACTB \u57fa\u56e0\u8bbe\u7f6e\u5185\u5bf9\u7167\uff08Internal Control, IC\uff09\u68c0\u6d4b\u5f15\u7269 \u63a2\u9488\uff0c \u5185\u5bf9\u7167\u63a2\u9488 5\u2019\u7aef\u6807\u8bb0 VIC \u8367\u5149\u7d20\uff0c ACTB \u57fa\u56e0\u4f5c\u4e3a\u5185\u5bf9\u7167\u57fa\u56e0\u7528\u6765 \u8bc4\u4f30\u5916\u5468\u8840 cfDNA \u7684\u63d0\u53d6\u8d28\u91cf\u3001\u4e9a\u786b\u9178\u6c22\u76d0\u7eaf\u5316\u8d28\u91cf\u548c PCR \u6269\u589e\u6548\u679c\u3002 \u8bd5\u5242\u76d2\u4e2d\u63d0\u4f9b\u9633\u6027\u5bf9\u7167\u548c\u9634\u6027\u5bf9\u7167\uff0c\u5728\u6bcf\u6b21\u68c0\u6d4b\u53cd\u5e94\u4e2d\u90fd\u9700\u8981\u540c\u65f6\u68c0 \u6d4b\uff0c\u786e\u4fdd\u8bd5\u9a8c\u7684\u6709\u6548\u6027\u3002 \u3010\u4e3b\u8981\u7ec4\u6210\u6210\u5206\u3011 1. \u8bd5\u5242\u76d2\u7684\u8bd5\u5242\u7ec4\u4efd \u8868 1 \u8bd5\u5242\u76d2\u7684\u4e3b\u8981\u7ec4\u5206 \u540d \u79f0 \u89c4\u683c \u6570\u91cf \u4e3b \u8981 \u6210 \u5206 SHOX2 PCR \u53cd\u5e94\u6db2 348\u00b5L/\u7ba1 1 \u7ba1 \n
\u51fa\u5904 [2] CH5.3\u4ea7\u54c1\u8bf4\u660e\u4e66.pdf\n \u5b9e\u65f6\u8367\u5149 PCR \u68c0\u6d4b\u662f\u6307\u5229\u7528\u5b9e\u65f6\u8367\u5149 PCR \u65b9\u6cd5\u7ed3\u5408\u4e0d\u540c\u8367\u5149\u6807\u8bb0\u7684 Taqman \u63a2\u9488\u5bf9\u4e9a\u786b\u9178\u6c22\u76d0\u7eaf\u5316\u540e\u7684 DNA \u8fdb\u884c\u68c0\u6d4b\u3002 \u6839\u636e SHOX2 \u548c PTGER4 \u57fa\u56e0\u542f\u52a8\u5b50\u5bcc\u542b\u9ad8 CpG \u4e8c\u8054\u4f53\u7532\u57fa\u5316\u5e8f\u5217\u8bbe\u8ba1\u5f15\u7269\u63a2\u9488\uff0c \u63a2\u9488 5\u2019 \u7aef\u5206\u522b\u6807\u8bb0 FAM \u548c ROX \u8367\u5149\u7d20\uff0c \u7ed3\u5408\u5316\u5b66\u4fee\u9970\u7684\u70ed\u542f\u52a8 Taq DNA \u805a\u5408 \u9176\u3001dNTPs \u7b49\u6210\u5206\u5bf9\u4e9a\u786b\u9178\u6c22\u76d0\u7eaf\u5316\u7684\u6838\u9178\u8fdb\u884c\u5b9a\u6027\u68c0\u6d4b\u3002 \u540c\u65f6\u68c0\u6d4b\u4f53\u7cfb\u4e2d \u8fd8\u6839\u636e\u4eba\u4fdd\u5b88\u57fa\u56e0 ACTB \u57fa\u56e0\u8bbe\u7f6e\u5185\u5bf9\u7167\uff08Internal Control, IC\uff09\u68c0\u6d4b\u5f15\u7269 \u63a2\u9488\uff0c \u5185\u5bf9\u7167\u63a2\u9488 5\u2019\u7aef\u6807\u8bb0 VIC \u8367\u5149\u7d20\uff0c ACTB \u57fa\u56e0\u4f5c\u4e3a\u5185\u5bf9\u7167\u57fa\u56e0\u7528\u6765 \u8bc4\u4f30\u5916\u5468\u8840 cfDNA \u7684\u63d0\u53d6\u8d28\u91cf\u3001\u4e9a\u786b\u9178\u6c22\u76d0\u7eaf\u5316\u8d28\u91cf\u548c PCR \u6269\u589e\u6548\u679c\u3002 \u8bd5\u5242\u76d2\u4e2d\u63d0\u4f9b\u9633\u6027\u5bf9\u7167\u548c\u9634\u6027\u5bf9\u7167\uff0c\u5728\u6bcf\u6b21\u68c0\u6d4b\u53cd\u5e94\u4e2d\u90fd\u9700\u8981\u540c\u65f6\u68c0 \u6d4b\uff0c\u786e\u4fdd\u8bd5\u9a8c\u7684\u6709\u6548\u6027\u3002 \u3010\u4e3b\u8981\u7ec4\u6210\u6210\u5206\u3011 1. \u8bd5\u5242\u76d2\u7684\u8bd5\u5242\u7ec4\u4efd \u8868 1 \u8bd5\u5242\u76d2\u7684\u4e3b\u8981\u7ec4\u5206 \u540d \u79f0 \u89c4\u683c \u6570\u91cf \u4e3b \u8981 \u6210 \u5206 SHOX2 PCR \u53cd\u5e94\u6db2 348\u00b5L/\u7ba1 1 \u7ba1 \n
\u51fa\u5904 [3] \u5b89\u9f99\u57fa\u56e0\u5546\u4e1a\u8ba1\u5212\u4e6620220111.pdf\n\u56fd\u5bb6\u836f\u54c1\u76d1\u7763\u7ba1\u7406\u5c40\u533b\u7597\u5668\u68b0\u6ce8\u518c\u7ba1\u7406\u53f8\uff1a \u5b89\u5fbd\u5b89\u9f99\u57fa\u56e0\u79d1\u6280\u6709\u9650\u516c\u53f8\u9488\u5bf9\u8fd1\u671f\u65b0\u578b\u51a0\u72b6\u75c5\u6bd2\u80ba\u708e\u75ab \u60c5\u7814\u5236\u4e86\u65b0\u578b\u51a0\u72b6\u75c5\u6bd2\uff082019-nCov\uff09\u68c0\u6d4b\u8bd5\u5242\u76d2\u3002 \u8be5\u516c\u53f8\u901a\u8fc7 \u8bd5\u5242\u76d2\u4e34\u5e8a\u6d4b\u8bd5\u6bd4\u5bf9\uff0c\u8ba4\u4e3a\u5176\u7814\u53d1\u7684\u8bd5\u5242\u76d2\u5177\u6709\u68c0\u6d4b\u901f\u5ea6\u5feb\u3001\u64cd \u4f5c\u4fbf\u643a\u6027\u3001\u9776\u57fa\u56e0\u6269\u589e\u4e00\u81f4\u6027\u3001\u7075\u654f\u5ea6\u9ad8\u7b49\u4f18\u52bf\u3002 \u4e3a\u8fdb\u4e00\u6b65\u6269\u5927 \u65b0\u578b\u51a0\u72b6\u75c5\u6bd2\u6838\u9178\u68c0\u6d4b\u8bd5\u5242\u4f9b\u7ed9\u80fd\u529b\uff0c\u5168\u529b\u670d\u52a1\u75ab\u60c5\u9632\u63a7\u9700\u8981\uff0c \u5b89\u5fbd\u5b89\u9f99\u57fa\u56e0\u79d1\u6280\u6709\u9650\u516c\u53f8\u7533\u8bf7\u56fd\u5bb6\u5c40\u5c06\u65b0\u578b\u51a0\u72b6\u75c5\u6bd2 \uff082019-nCov\uff09\u68c0\u6d4b\u8bd5\u5242\u76d2\u6309\u7167\u5e94\u6025\u5ba1\u6279\u7a0b\u5e8f\u6279\u51c6\u4e0a\u5e02\u3002 \u73b0\u5c06\u4f01 \u4e1a\u76f8\u5173\u6587\u4ef6\u968f\u6587\u8f6c\u62a5\uff0c\u8bf7\u7ed9\u4e88\u652f\u6301\u3002 \n
"], ["SHOX2/PTGER4 \u57fa\u56e0\u7532\u57fa\u5316\u68c0\u6d4b\u8bd5\u5242\u76d2\u7684\u8bf4\u660e\u4e66\u7684\u53c2\u8003\u6587\u732e\u6709\u54ea\u4e9b\uff1f", "

\u6839\u636e\u5df2\u77e5\u4fe1\u606f\uff0cSHOX2/PTGER4\u57fa\u56e0\u7532\u57fa\u5316\u68c0\u6d4b\u8bd5\u5242\u76d2\u7684\u8bf4\u660e\u4e66\u53c2\u8003\u6587\u732e\u6709\uff1a

\n
    \n
  1. SHOX2/PTGER4\u57fa\u56e0\u7532\u57fa\u5316\u68c0\u6d4b\u8bd5\u5242\u76d2\uff08PCR\u8367\u5149\u6cd5\uff09\u8bf4\u660e\u4e66\u3002
    2. \n
  2. \u8bd5\u5242\u76d2\u4ea7\u54c1\u8bf4\u660e\u4e66\u3002
    3. \n
  3. \u5b89\u9f99\u57fa\u56e0\u5546\u4e1a\u8ba1\u5212\u4e6620220111.pdf
    4. \n
  4. 2019\u5e74\u5168\u56fd\u6700\u65b0\u7684\u764c\u75c7\u62a5\u544a\u3002
    5. \n
  5. \u7b2c\u4e00\u5c4a\u80bf\u7624\u7cbe\u51c6\u533b\u7597\u4e0e\u6db2\u4f53\u6d3b\u68c0\u884c\u4e1a\u9ad8\u5cf0\u8bba\u575b\uff0c\u66a8\u5e7fFONA\u7532\u57fa\u5316\u5728\u80ba\u7ed3\u8282\u826f\u60e0\u6027\u8bca\u65ad\u4e34\u9ebb\u4ef7\u503c\u4e0e\u4e34\u5c4a\u8bd5\u9a8c\u65b9\u6848\u63a2\u8fc7\u4f1a\u4eea\u3002
    6. \n
  6. \u5f90\u5dde\u533b\u79d1\u5927\u5b66\u9644\u5c5e\u533b\u9662\u533b\u5b66\u4f26\u7406\u59d4\u5458\u4f1a\u4f26\u7406\u5ba1\u67e5\u610f\u89c1\u3002
    7. \n
  7. \u5317\u4eac\u5e02\u6d77\u6dc0\u533b\u9662\u533b\u5b66\u4f26\u7406\u59d4\u5458\u4f1a\u5b66\u672f\u53ca\u4f26\u7406\u5ba1\u67e5\u610f\u89c1\u51fd\u3002
    8. \n
\n

\u4ee5\u4e0a\u53c2\u8003\u6587\u732e\u65e0\u6cd5\u63d0\u4f9b\u66f4\u591a\u76f8\u5173\u5185\u5bb9\uff0c\u56de\u7b54\u8be5\u95ee\u9898\u3002

\n
\u51fa\u5904 [1] CH5.3\u4ea7\u54c1\u8bf4\u660e\u4e66.pdf\n\u9634\u6027\u5bf9\u7167\uff08NC\uff09Ct \u503c\u4e0d\u51fa\u73b0\u4efb\u4f55\u6570\u503c\u3002 \u8fdb \u4e00\u6b65\u5206\u6790\u9633\u6027\u5bf9\u7167\u7684 Ct \u503c\uff0c\u4f7f\u9633\u6027\u5bf9\u7167\u7684 Ct \u503c\u6ee1\u8db3 26\u2264Ct \u503c\u226429\uff1b \u4e0d\u540c\u4eea \u5668\u4e0a\u5c3d\u53ef\u80fd\u901a\u8fc7\u8c03\u6574\u4eea\u5668\u7684\u201c\u9608\u503c\u7ebf\u201d\uff0c\u4f7f\u5f97\u9633\u6027\u5bf9\u7167\u7684 FAM \u548c ROX \u901a\u9053 Ct \u503c\u5747\u63a5\u8fd1 28\u3002 \u3010\u9633\u6027\u5224\u65ad\u503c\u3011 \u4f7f\u7528\u4eba SHOX2/PTGER4 \u57fa\u56e0\u7532\u57fa\u5316\u68c0\u6d4b\u8bd5\u5242\u76d2\uff08PCR \u8367\u5149\u6cd5\uff09\u5bf9\u4e34\u5e8a \u6837\u672c\u7684 SHOX2 \u548c PTGER4 \u57fa\u56e0\u8fdb\u884c\u7532\u57fa\u5316\u68c0\u6d4b\uff0c \u5168\u90e8\u4e34\u5e8a\u6837\u672c\u7684 ACTB \u57fa \u56e0 Ct \u503c\u8303\u56f4\u662f 24. 24~42. 00 \u4e4b\u95f4\uff0c\u53d6 97.\n
\u51fa\u5904 [2] CH5.3\u4ea7\u54c1\u8bf4\u660e\u4e66.pdf\nS8 PC P8 PC \u4eba SHOX2/PTGER4 \u57fa\u56e0\u7532\u57fa\u5316\u68c0\u6d4b\u8bd5\u5242\u76d2\uff08PCR \u8367\u5149\u6cd5\uff09\u8bf4\u660e\u4e66 \u8d27\u53f7\uff1aAL-LC01-24 V2020. 3. 10 3 / 3 \u5b89\u5fbd\u5b89\u9f99\u57fa\u56e0\u79d1\u6280\u6709\u9650\u516c\u53f8 2. \u4e3a\u4e86\u907f\u514d\u6837\u672c\u4e2d\u4efb\u4f55\u6f5c\u5728\u7684\u751f\u7269\u5371\u9669\uff0c\u8bd5\u5242\u76d2\u7ec4\u5206\u53ca\u68c0\u6d4b\u6837\u54c1\u5e94\u89c6\u4e3a\u5177\u6709 \u4f20\u67d3\u6027\u7269\u8d28\uff0c\u907f\u514d\u63a5\u89e6\u5230\u76ae\u80a4\u548c\u7c98\u819c\u3002\n
\u51fa\u5904 [3] \u5b89\u9f99\u57fa\u56e0\u5546\u4e1a\u8ba1\u5212\u4e6620220111.pdf\n51% 3. 88% \u98df\u7ba1\u764c \u6dcb\u5df4\u764c 2. 42% 3. 88% \u5b50\u5bab\u4f53\u764c \u8111\u764c 2. 32% 3. 21% \u8111\u764c \u6570\u636e\u6765\u81ea\uff1a2019\u5e74\u5168\u56fd\u6700\u65b0\u7684\u764c\u75c7\u62a5\u544a\u80ba\u7ef4\u6b23TM\u4e34\u5e8a\u7533\u62a5\u8fdb\u5ea6 \u7b2c\u4e00\u5c4a\u80bf\u7624\u7cbe\u51c6\u533b\u7597\u4e0e\u6db2\u4f53\u6d3b\u68c0\u884c\u4e1a\u9ad8\u5cf0\u8bba\u575b \u66a8\u5e7fFONA\u7532\u57fa\u5316\u5728\u80ba\u7ed3\u8282\u826f\u60e0\u6027\u8bca\u65ad\u4e34\u9ebb\u4ef7\u503c\u4e0e\u4e34\u5c4a\u8bd5\u9a8c\u65b9\u6848\u63a2\u8fc7\u4f1a\u4eea \u5b89\u9f99\u57fa\u56e0\u80ba\u7ef4\u6b23 PROLUNGCA\u6c5f\u82cf \u4ebaSHOX2/PTGER4\u57fa\u56e0\u7532\u57fa\u5316\u68c0\u6d4b \u5b89\u5fbd\u5b89\u9f99\u57fa\u56e0\u79d1\u6280\u6709\u9650\u516c\u53f8 \u68c0\u9a8c\u62a5\u544a \u8bd5\u5242\u76d2\uff08PCR\u8367\u5149\u6cd5\uff09 \u6c5f\u82cf\u7701\u533b\u7597\u5668\u68b0\u68c0\u9a8c\u6240 24\u6d4b\u8bd5/\u76d2 Test Report (2020\uff09QW \u79f0 \u89c4\u683c\u578b\u53f7 Specifications \u68c0\u9a8c\u7c7b\u522b \u59d4\u6258\u5355\u4f4d\u5f90\u5dde\u533b\u79d1\u5927\u5b66\u9644\u5c5e\u533b\u9662\u533b\u5b66\u4f26\u7406\u59d4\u5458\u4f1a \u4f26\u7406\u5ba1\u67e5\u610f\u89c1 \u610f\u89c1\u53f7 XYFY2020 QL131 01 \u9879\u76ee\u540d\u79f0 \u4ebaSHOX2/PTGER4\u57fa\u56e0\u7532\u57fa\u5316\u68c0\u6d4b\u8bd5\u5242\u76d2\uff08PCR\u8367\u5149\u6cd5\uff09 \u7814\u7a76\u673a\u6784 \u5f90\u5dde\u533b\u79d1\u5927\u5b66\u9644\u5c5e\u533b\u9662 \u7814\u7a76\u79d1\u5ba4 \u75c5\u7406\u79d1 \u4e3b\u8981\u7814\u7a76\u8005 \u5218\u6167 \u804c\u79f0 \u4e3b\u4efb\u533b\u5e08 \u5ba1\u67e5\u7c7b\u522b \u521d\u59cb\u5ba1\u67e5 \u5ba1\u67e5\u65b9\u5f0f \u5feb\u901f\u5ba1\u67e5 2020 11 23,\n
\u51fa\u5904 [4] \u5b89\u9f99\u57fa\u56e0\u5546\u4e1a\u8ba1\u5212\u4e6620220111.pdf\n20\u3001\u7814\u7a76\u8005\u4fdd\u8bc1\u6240\u63d0\u4f9b\u8d44\u6599\u771f\u5b9e\u6027\u7684\u58f0\u660e \u5ba1\u67e5\u610f\u89c1\u5317\u4eac\u5e02\u6d77\u6dc0\u533b\u9662\u533b\u5b66\u4f26\u7406\u59d4\u5458\u4f1a BeijingHaidianHospital MedicalEthics Committee \u5b66\u672f\u53ca\u4f26\u7406\u5ba1\u67e5\u610f\u89c1\u51fd/\u6279\u4ef6 \uff082020\uff09\u836f/\u533b\u4f26\u5ba1\u7b2c\uff0845\uff09\u53f7 \u9879\u76ee\u7f16\u53f7 2020005 \u4e34\u5e8a\u8bd5\u9a8c\u9879\u76ee\u540d\u79f0 \u4ebaSHOX2/PTGER4\u57fa\u56e0\u7532\u57fa\u5316\u68c0\u6d4b\u8bd5\u5242\u76d2\uff08PCR\u8367\u5149\u6cd5\uff09 \u56e2\u836f\u7269/\u5668\u68b0\u4e34\u5e8a\u8bd5\u9a8c \u53e3\u529f\u80fd\u98df\u54c1/\u4fdd\u5065\u54c1 \u53e3\u7814\u7a76\u8005\u81ea\u53d1 \u9879\u76ee\u6765\u6e90 \u53e3\u4f01\u4e1a\u8d44\u52a9 \u53e3\u6a2a\u5411\u5408\u4f5c \u53e3\u5728\u7814\u8bfe\u9898 \u53e3\u8bfe\u9898\u7533\u62a5\uff1a \n
"], ["SHOX2/PTGER4 \u57fa\u56e0\u7532\u57fa\u5316\u68c0\u6d4b\u8bd5\u5242\u76d2\u4ea7\u54c1\u8bf4\u660e\u4e66\u7684\u53c2\u8003\u6587\u732e\u5305\u62ec\u54ea\u4e9b\uff1f", "

\u6839\u636e\u5df2\u77e5\u4fe1\u606f\uff0cSHOX2/PTGER4\u57fa\u56e0\u7532\u57fa\u5316\u68c0\u6d4b\u8bd5\u5242\u76d2\u4ea7\u54c1\u8bf4\u660e\u4e66\u7684\u53c2\u8003\u6587\u732e\u6709\uff1a

\n
    \n
  1. SHOX2/PTGER4\u57fa\u56e0\u7532\u57fa\u5316\u68c0\u6d4b\u8bd5\u5242\u76d2\uff08PCR\u8367\u5149\u6cd5\uff09\u8bf4\u660e\u4e66\u3002
    2. \n
  2. \u8bd5\u5242\u76d2\u4ea7\u54c1\u8bf4\u660e\u4e66\u3002
    3. \n
  3. \u5b89\u9f99\u57fa\u56e0\u5546\u4e1a\u8ba1\u5212\u4e6620220111.pdf
    4. \n
  4. 2019\u5e74\u5168\u56fd\u6700\u65b0\u7684\u764c\u75c7\u62a5\u544a\u3002
    5. \n
  5. \u7b2c\u4e00\u5c4a\u80bf\u7624\u7cbe\u51c6\u533b\u7597\u4e0e\u6db2\u4f53\u6d3b\u68c0\u884c\u4e1a\u9ad8\u5cf0\u8bba\u575b\uff0c\u66a8\u5e7fFONA\u7532\u57fa\u5316\u5728\u80ba\u7ed3\u8282\u826f\u60e0\u6027\u8bca\u65ad\u4e34\u9ebb\u4ef7\u503c\u4e0e\u4e34\u5c4a\u8bd5\u9a8c\u65b9\u6848\u63a2\u8fc7\u4f1a\u4eea\u3002
    6. \n
  6. \u5b89\u9f99\u57fa\u56e0\u80ba\u7ef4\u6b23\u3002
    7. \n
  7. \u5f90\u5dde\u533b\u79d1\u5927\u5b66\u9644\u5c5e\u533b\u9662\u533b\u5b66\u4f26\u7406\u59d4\u5458\u4f1a\u4f26\u7406\u5ba1\u67e5\u610f\u89c1\u3002
    8. \n
  8. \u5317\u4eac\u5e02\u6d77\u6dc0\u533b\u9662\u533b\u5b66\u4f26\u7406\u59d4\u5458\u4f1a\u5b66\u672f\u53ca\u4f26\u7406\u5ba1\u67e5\u610f\u89c1\u51fd\u3002
    9. \n
\n

\u4ee5\u4e0a\u53c2\u8003\u6587\u732e\u65e0\u6cd5\u63d0\u4f9b\u66f4\u591a\u76f8\u5173\u5185\u5bb9\uff0c\u56de\u7b54\u8be5\u95ee\u9898\u3002

\n
\u51fa\u5904 [1] CH5.3\u4ea7\u54c1\u8bf4\u660e\u4e66.pdf\n\u9634\u6027\u5bf9\u7167\uff08NC\uff09Ct \u503c\u4e0d\u51fa\u73b0\u4efb\u4f55\u6570\u503c\u3002 \u8fdb \u4e00\u6b65\u5206\u6790\u9633\u6027\u5bf9\u7167\u7684 Ct \u503c\uff0c\u4f7f\u9633\u6027\u5bf9\u7167\u7684 Ct \u503c\u6ee1\u8db3 26\u2264Ct \u503c\u226429\uff1b \u4e0d\u540c\u4eea \u5668\u4e0a\u5c3d\u53ef\u80fd\u901a\u8fc7\u8c03\u6574\u4eea\u5668\u7684\u201c\u9608\u503c\u7ebf\u201d\uff0c\u4f7f\u5f97\u9633\u6027\u5bf9\u7167\u7684 FAM \u548c ROX \u901a\u9053 Ct \u503c\u5747\u63a5\u8fd1 28\u3002 \u3010\u9633\u6027\u5224\u65ad\u503c\u3011 \u4f7f\u7528\u4eba SHOX2/PTGER4 \u57fa\u56e0\u7532\u57fa\u5316\u68c0\u6d4b\u8bd5\u5242\u76d2\uff08PCR \u8367\u5149\u6cd5\uff09\u5bf9\u4e34\u5e8a \u6837\u672c\u7684 SHOX2 \u548c PTGER4 \u57fa\u56e0\u8fdb\u884c\u7532\u57fa\u5316\u68c0\u6d4b\uff0c \u5168\u90e8\u4e34\u5e8a\u6837\u672c\u7684 ACTB \u57fa \u56e0 Ct \u503c\u8303\u56f4\u662f 24. 24~42. 00 \u4e4b\u95f4\uff0c\u53d6 97.\n
\u51fa\u5904 [2] CH5.3\u4ea7\u54c1\u8bf4\u660e\u4e66.pdf\nS8 PC P8 PC \u4eba SHOX2/PTGER4 \u57fa\u56e0\u7532\u57fa\u5316\u68c0\u6d4b\u8bd5\u5242\u76d2\uff08PCR \u8367\u5149\u6cd5\uff09\u8bf4\u660e\u4e66 \u8d27\u53f7\uff1aAL-LC01-24 V2020. 3. 10 3 / 3 \u5b89\u5fbd\u5b89\u9f99\u57fa\u56e0\u79d1\u6280\u6709\u9650\u516c\u53f8 2. \u4e3a\u4e86\u907f\u514d\u6837\u672c\u4e2d\u4efb\u4f55\u6f5c\u5728\u7684\u751f\u7269\u5371\u9669\uff0c\u8bd5\u5242\u76d2\u7ec4\u5206\u53ca\u68c0\u6d4b\u6837\u54c1\u5e94\u89c6\u4e3a\u5177\u6709 \u4f20\u67d3\u6027\u7269\u8d28\uff0c\u907f\u514d\u63a5\u89e6\u5230\u76ae\u80a4\u548c\u7c98\u819c\u3002\n
\u51fa\u5904 [3] \u5b89\u9f99\u57fa\u56e0\u5546\u4e1a\u8ba1\u5212\u4e6620220111.pdf\n51% 3. 88% \u98df\u7ba1\u764c \u6dcb\u5df4\u764c 2. 42% 3. 88% \u5b50\u5bab\u4f53\u764c \u8111\u764c 2. 32% 3. 21% \u8111\u764c \u6570\u636e\u6765\u81ea\uff1a2019\u5e74\u5168\u56fd\u6700\u65b0\u7684\u764c\u75c7\u62a5\u544a\u80ba\u7ef4\u6b23TM\u4e34\u5e8a\u7533\u62a5\u8fdb\u5ea6 \u7b2c\u4e00\u5c4a\u80bf\u7624\u7cbe\u51c6\u533b\u7597\u4e0e\u6db2\u4f53\u6d3b\u68c0\u884c\u4e1a\u9ad8\u5cf0\u8bba\u575b \u66a8\u5e7fFONA\u7532\u57fa\u5316\u5728\u80ba\u7ed3\u8282\u826f\u60e0\u6027\u8bca\u65ad\u4e34\u9ebb\u4ef7\u503c\u4e0e\u4e34\u5c4a\u8bd5\u9a8c\u65b9\u6848\u63a2\u8fc7\u4f1a\u4eea \u5b89\u9f99\u57fa\u56e0\u80ba\u7ef4\u6b23 PROLUNGCA\u6c5f\u82cf \u4ebaSHOX2/PTGER4\u57fa\u56e0\u7532\u57fa\u5316\u68c0\u6d4b \u5b89\u5fbd\u5b89\u9f99\u57fa\u56e0\u79d1\u6280\u6709\u9650\u516c\u53f8 \u68c0\u9a8c\u62a5\u544a \u8bd5\u5242\u76d2\uff08PCR\u8367\u5149\u6cd5\uff09 \u6c5f\u82cf\u7701\u533b\u7597\u5668\u68b0\u68c0\u9a8c\u6240 24\u6d4b\u8bd5/\u76d2 Test Report (2020\uff09QW \u79f0 \u89c4\u683c\u578b\u53f7 Specifications \u68c0\u9a8c\u7c7b\u522b \u59d4\u6258\u5355\u4f4d\u5f90\u5dde\u533b\u79d1\u5927\u5b66\u9644\u5c5e\u533b\u9662\u533b\u5b66\u4f26\u7406\u59d4\u5458\u4f1a \u4f26\u7406\u5ba1\u67e5\u610f\u89c1 \u610f\u89c1\u53f7 XYFY2020 QL131 01 \u9879\u76ee\u540d\u79f0 \u4ebaSHOX2/PTGER4\u57fa\u56e0\u7532\u57fa\u5316\u68c0\u6d4b\u8bd5\u5242\u76d2\uff08PCR\u8367\u5149\u6cd5\uff09 \u7814\u7a76\u673a\u6784 \u5f90\u5dde\u533b\u79d1\u5927\u5b66\u9644\u5c5e\u533b\u9662 \u7814\u7a76\u79d1\u5ba4 \u75c5\u7406\u79d1 \u4e3b\u8981\u7814\u7a76\u8005 \u5218\u6167 \u804c\u79f0 \u4e3b\u4efb\u533b\u5e08 \u5ba1\u67e5\u7c7b\u522b \u521d\u59cb\u5ba1\u67e5 \u5ba1\u67e5\u65b9\u5f0f \u5feb\u901f\u5ba1\u67e5 2020 11 23,\n
\u51fa\u5904 [4] \u5b89\u9f99\u57fa\u56e0\u5546\u4e1a\u8ba1\u5212\u4e6620220111.pdf\n20\u3001\u7814\u7a76\u8005\u4fdd\u8bc1\u6240\u63d0\u4f9b\u8d44\u6599\u771f\u5b9e\u6027\u7684\u58f0\u660e \u5ba1\u67e5\u610f\u89c1\u5317\u4eac\u5e02\u6d77\u6dc0\u533b\u9662\u533b\u5b66\u4f26\u7406\u59d4\u5458\u4f1a BeijingHaidianHospital MedicalEthics Committee \u5b66\u672f\u53ca\u4f26\u7406\u5ba1\u67e5\u610f\u89c1\u51fd/\u6279\u4ef6 \uff082020\uff09\u836f/\u533b\u4f26\u5ba1\u7b2c\uff0845\uff09\u53f7 \u9879\u76ee\u7f16\u53f7 2020005 \u4e34\u5e8a\u8bd5\u9a8c\u9879\u76ee\u540d\u79f0 \u4ebaSHOX2/PTGER4\u57fa\u56e0\u7532\u57fa\u5316\u68c0\u6d4b\u8bd5\u5242\u76d2\uff08PCR\u8367\u5149\u6cd5\uff09 \u56e2\u836f\u7269/\u5668\u68b0\u4e34\u5e8a\u8bd5\u9a8c \u53e3\u529f\u80fd\u98df\u54c1/\u4fdd\u5065\u54c1 \u53e3\u7814\u7a76\u8005\u81ea\u53d1 \u9879\u76ee\u6765\u6e90 \u53e3\u4f01\u4e1a\u8d44\u52a9 \u53e3\u6a2a\u5411\u5408\u4f5c \u53e3\u5728\u7814\u8bfe\u9898 \u53e3\u8bfe\u9898\u7533\u62a5\uff1a \n
"], ["\u4ea7\u54c1\u8bf4\u660e\u4e66\u7684\u53c2\u8003\u6587\u732e\u6709\u54ea\u4e9b\uff1f", "\u4ea7\u54c1\u8bf4\u660e\u4e66\u7684\u53c2\u8003\u6587\u732e\u901a\u5e38\u5305\u62ec\uff1a\n\n1. \u4ea7\u54c1\u8bf4\u660e\u4e66\uff1a\u8fd9\u662f\u4ea7\u54c1\u8bf4\u660e\u4e66\u7684\u4e3b\u8981\u53c2\u8003\u6587\u732e\uff0c\u5e94\u8be5\u5305\u62ec\u4ea7\u54c1\u7684\u540d\u79f0\u3001\u578b\u53f7\u3001\u89c4\u683c\u3001\u6027\u80fd\u3001\u7528\u9014\u3001\u4f7f\u7528\u65b9\u6cd5\u3001\u6ce8\u610f\u4e8b\u9879\u3001\u751f\u4ea7\u5382\u5bb6\u3001\u751f\u4ea7\u65e5\u671f\u3001\u6279\u53f7\u3001\u6709\u6548\u671f\u7b49\u4fe1\u606f\u3002\n\n2. \u6280\u672f\u89c4\u683c\u4e66\uff1a\u8fd9\u662f\u4ea7\u54c1\u6280\u672f\u89c4\u683c\u7684\u4e3b\u8981\u53c2\u8003\u6587\u732e\uff0c\u5e94\u8be5\u5305\u62ec\u4ea7\u54c1\u7684\u6280\u672f\u89c4\u683c\u3001\u6280\u672f\u8981\u6c42\u3001\u6280\u672f\u53c2\u6570\u3001\u6280\u672f\u6570\u636e\u7b49\u5185\u5bb9\u3002\n\n3. \u7528\u6237\u624b\u518c\uff1a\u8fd9\u662f\u4ea7\u54c1\u7528\u6237\u624b\u518c\u7684\u4e3b\u8981\u53c2\u8003\u6587\u732e\uff0c\u5e94\u8be5\u5305\u62ec\u4ea7\u54c1\u7684\u4f7f\u7528\u8bf4\u660e\u3001\u64cd\u4f5c\u6307\u5357\u3001\u7ef4\u62a4\u4fdd\u517b\u3001\u6545\u969c\u6392\u9664\u7b49\u5185\u5bb9\u3002\n\n4. \u8bf4\u660e\u4e66\u4e2d\u7684\u56fe\u8868\uff1a\u8fd9\u4e9b\u56fe\u8868\u662f\u4ea7\u54c1\u8bf4\u660e\u4e66\u4e2d\u7684\u91cd\u8981\u53c2\u8003\u6587\u732e\uff0c\u5e94\u8be5\u5305\u62ec\u4ea7\u54c1\u7684\u7ed3\u6784\u56fe\u3001\u539f\u7406\u56fe\u3001\u6d41\u7a0b\u56fe\u3001\u793a\u610f\u56fe\u7b49\u3002\n\n5. \u4ea7\u54c1\u8bf4\u660e\u4e66\u4e2d\u7684\u53c2\u8003\u6587\u732e\uff1a\u8fd9\u4e9b\u53c2\u8003\u6587\u732e\u662f\u4ea7\u54c1\u8bf4\u660e\u4e66\u4e2d\u7684\u91cd\u8981\u53c2\u8003\u6587\u732e\uff0c\u5e94\u8be5\u5305\u62ec\u4e0e\u4ea7\u54c1\u76f8\u5173\u7684\u6587\u732e\u3001\u6cd5\u89c4\u3001\u6807\u51c6\u7b49\u3002\n\n6. \u4ea7\u54c1\u8bf4\u660e\u4e66\u4e2d\u7684\u5176\u4ed6\u8d44\u6599\uff1a\u8fd9\u4e9b\u8d44\u6599\u662f\u4ea7\u54c1\u8bf4\u660e\u4e66\u4e2d\u7684\u5176\u4ed6\u53c2\u8003\u6587\u732e\uff0c\u5e94\u8be5\u5305\u62ec\u4ea7\u54c1\u7684\u5b89\u5168\u6027\u8bc4\u4ef7\u3001\u6709\u6548\u6027\u8bc4\u4ef7\u3001\u4e34\u5e8a\u8bd5\u9a8c\u7ed3\u679c\u3001\u5e02\u573a\u8c03\u7814\u7ed3\u679c\u7b49\u3002\n\n\u5177\u4f53\u7684\u53c2\u8003\u6587\u732e\u5e94\u8be5\u6839\u636e\u4ea7\u54c1\u7684\u5b9e\u9645\u60c5\u51b5\u8fdb\u884c\u9009\u62e9\uff0c\u4ee5\u786e\u4fdd\u4ea7\u54c1\u7684\u8bf4\u660e\u4e66\u5185\u5bb9\u5b8c\u6574\u3001\u51c6\u786e\u3001\u53ef\u9760\u3002\n\n"]]