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Translocation, t(17;22)(q22;q13), in dermatofibrosarcoma protuberans: a new tumor-associated chromosome rearrangement. | A translocation; t(17;22)(q22;q13); was identified in two cases of dermatofibrosarcoma protuberans (DP). They bring to four the number of DP cases characterized by an identical t(17;22)(q22;q13); which can be considered as a new tumor-associated chromosome rearrangement. To date; this translocation has been found only in DP and its juvenile form; giant-cell fibroblastoma. This finding has two major consequences. First; it casts light on the development and significance in DP of ring chromosomes which consis | 8978765 | [
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Translocation, t(17;22)(q22;q13), in dermatofibrosarcoma protuberans: a new tumor-associated chromosome rearrangement. | tently harbor sequences derived from chromosomes 17 and 22. Second; the identification of this new chromosome marker; and eventually of the underlying molecular rearrangement; should help to classify DP; a soft-tissue tumor of still uncertain cell origin. In addition; it could be used to differentiate DP from truly benign or malignant entities; in order that this tumor of intermediate malignancy could be adequately managed. | 8978765 | [
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Colcemid increases the frequency of chromosome abnormalities in human sperm. | The effect of Colcemid on the frequency and type of chromosomal abnormalities in human sperm was investigated. Using the human sperm and zona-free hamster egg fusion technique; penetrated eggs were cultured in the presence or absence of Colcemid. We used two different times of Colcemid treatment: standard Colcemid treatment (Colcemid-5 h) or long Colcemid treatment (Colcemid-17 h). Each Colcemid series had its own control series without Colcemid; thus ensuring that Colcemid was the only significant variable | 8978764 | [
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Colcemid increases the frequency of chromosome abnormalities in human sperm. | . A total of 771 sperm karyotypes from one normal donor was analyzed: 286 in the Colcemid-5 h series; 262 in the Colcemid-17 h series; and 223 in the two control series. In both Colcemid series there was a significant increase in the frequencies of hypohaploidy vs. hyperhaploidy (9.4% and 7.3% vs. 2.4% and 1.1%; for the Colcemid-5 h and Colcemid-17 h series; respectively); in contrast to those obtained in the control series; in which the frequencies of hypohaploidy and hyperhaploidy were close to the 1:1 re | 8978764 | [
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Colcemid increases the frequency of chromosome abnormalities in human sperm. | lationship (4.9% vs. 4.0%) expected from nondisjunction. There was a significant increase in the frequency of structural abnormalities in both Colcemid series (16.1% and 14.5% for the Colcemid-5 h and Colcemid-17 h series; respectively) compared to the control series (6.3%). These results suggest that Colcemid significantly increases the frequency of hypohaploidy and unstable structural aberrations in human sperm. | 8978764 | [
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Assignment of the human beta-microseminoprotein gene (MSMB) to chromosome 10q11.2. | Beta-microseminoprotein (MSP) is one of the major proteins secreted by the prostate; and its biological role in tumorigenesis of the prostate has been postulated. We assigned the human MSP gene (MSMB) to 10q11.2 with fluorescence in situ hybridization using a phage clone that has an MSP gene insert. Our mapping data shows that the gene is outside the previously identified LOH-regions (10p and 10q24-->qter) in prostate cancer cells and indicates that MSMB can be ruled out as a candidate for a tumor suppresso | 8978767 | [
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Deletion of the SRY region on the Y chromosome detected in bovine gonadal hypoplasia (XY female) by PCR. | In 6 sterile heifers which were female in appearance and in genital organs; all tissues derived from blood; skin; spleen and kidney had a single 60;XY type of metaphase plate. Although the SRY gene was detected by PCR in normal bull; it was not detected in normal cow or in 3 60;XY cases studied. | 8978769 | [
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Assignment of connexin 26 (GJB2) and 46 (GJA3) genes to human chromosome 13q11-->q12 and mouse chromosome 14D1-E1 by in situ hybridization. | nan | 8978770 | [
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Mapping of the metalloproteinase gene matrilysin (MMP7) to human chromosome 11q21-->q22. | The matrix metalloproteinase; matrilysin; is thought to play an important role in the early steps of tumor progression. We determined the chromosome location of the matrilysin gene (MMP7) by Southern and PCR analysis of two different panels of somatic cell hybrids and in situ hybridization of metaphase chromosomes. Matrilysin maps to the region; 11q21-->q22; adding MMP7 to the cluster of matrix metalloproteinase genes that have already mapped to this region. | 8978768 | [
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Reassignment of MYCL1 to human chromosome 1p34.3 by fluorescence in situ hybridization. | MYCL1 has been previously mapped to 1p32. This position was in contradiction with our mapping data of 1p deletions in neuroblastoma cell lines. Using FISH on high resolution R-banded chromosomes with a YAC clone for MYCL1 we were able to reassign the gene to subband 1p34.3. This new map position is of importance for the establishment of an integrated map for chromosome 1 and the search for disease genes in this region. | 8978772 | [
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Cloning and mapping of a human novel cDNA (NHP2L1) that encodes a protein highly homologous to yeast nuclear protein NHP2. | We have isolated a novel human cDNA that encodes a protein highly homologous to NHP2; a nuclear protein of yeast; that is thought to have an essential physiological function for cell viability. The 1;493-bp cDNA sequence includes an open reading frame of 384 bp. This gene (NHP2L1) was expressed in all human tissues examined; and was localized to chromosome hand 12q24.3 by fluorescence in situ hybridization. | 8978773 | [
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Correction of the spontaneous and DEB-induced chromosomal aberrations in Fanconi anemia cells of the FA(C) complementation group by the FACC gene. | Fanconi anemia (FA) cells are hypersensitive to the cytotoxic and clastogenic effects of DNA cross-linking agents. Four complementation groups have been identified to date. The gene (FACC) that corrects the hypersensitivity of one of them; FA(C); has been cloned. In the present study; both the increased spontaneous and diepoxybutane (DEB)-induced chromosomal instability in FA(C) lymphoblastoid cells were corrected by transfection of FACC. | 8978774 | [
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Close linkage between RNR and GPD genes on the tammar wallaby (Macropus eugenii) X chromosome. | No recombination was detected between two X-linked loci; RNR (Xp) and GPD (Xq); among 69 backcross progeny of two distantly related tammar wallaby (Macropus eugenii) subspecies. RNR loci are not dosage compensated; whereas the GPD locus is subject to the paternal X chromosome inactivation system that characterises female marsupials. The border of the region controlled by the marsupial X chromosome inactivation system has therefore been shown to lie between RNR and GPD. | 8978775 | [
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Metaphase and interphase mapping by FISH: improvement of chromosome banding and signal resolution in interphase nuclei by means of iterative deconvolution. | FISH images obtained with conventional epifluorescence microscopes are always blurred by glare and out of focus light emissions. In order to restore high contrast images; a procedure based on a modelling of the optical system in the microscope was developed and used for the processing of images acquired with a cooled CCD camera mounted on a fluorescence microscope. This procedure was tested on images of both mouse and human chromosomes stained with DAP1 and on images of interphase nuclei hybridized with pai | 8978776 | [
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Metaphase and interphase mapping by FISH: improvement of chromosome banding and signal resolution in interphase nuclei by means of iterative deconvolution. | rs of cosmid probes. This method improves the definition and the sharpness of the DAPI G-banding and thus facilitates and speeds up the identification of chromosomes. When performed on images of interphase cell nuclei; this procedure allows the discrimination of fluorescent signals which appear partially overlapping on raw images. This significant improvement of spatial resolution is of particular interest for ordering sets of probes on DNA fibers. | 8978776 | [
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Regional mapping of the human platelet-activating factor receptor gene (PTAFR) to 1p35-->p34.3 by fluorescence in situ hybridization. | The human platelet-activating factor cell-surface receptor (PTAFR) is a G protein-coupled receptor thought to contribute to many atopic and inflammatory diseases and; perhaps; to the growth of some neoplasms. Exploring the possibility that the PTAFR might be involved in the genetic predisposition to any disease requires knowledge of its chromosomal localization. In this paper we have used a 20-kb human genomic fragment containing the coding sequence of the cloned PTAFR to determine the regional chromosomal | 8978777 | [
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Regional mapping of the human platelet-activating factor receptor gene (PTAFR) to 1p35-->p34.3 by fluorescence in situ hybridization. | localization of the gene. Using fluorescence in situ hybridization; the localization of the human PTAFR gene was mapped to 1p35-->p34.3. | 8978777 | [
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Cloning, expression, and mapping of CKAPI, which encodes a putative cytoskeleton-associated protein containing a CAP-GLY domain. | From a human fetal-brain cDNA library we isolated a novel cDNA clone encoding a protein containing a CAP-GLY domain that is highly conserved among several cytoskeleton-associated proteins. The CAP-GLY domain is thought to be essential for their association with microtubules. The cDNA; designated CKAPI (for cytoskeleton-associated protein I; glycine motif) contained an open reading frame of 579 nucleotides encoding 193 amino acids. Northern-blot analysis revealed expression of three transcripts; 1.0; 3.4; an | 8978778 | [
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Cloning, expression, and mapping of CKAPI, which encodes a putative cytoskeleton-associated protein containing a CAP-GLY domain. | d 4.6 kb in size; in all tissues examined. The 1.0-kb transcript was significantly higher in brain and heart than in other tissues. This gene was mapped by FISH to chromosome bands 19q13.11-->q13.12. | 8978778 | [
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Synteny conservation between parts of human chromosome 4q and bovine and ovine chromosomes 6. | Booroola Merino sheep are characterized by a high ovulation rate attributable to the presence of the FecB allele of the FEC gene. This gene; which has been assigned to sheep chromosome 6; is linked to the gene for EGF; which in man is located on the long arm of chromosome 4 (HSA 4q). To increase the number of known markers on sheep chromosome 6; we used comparative mapping data from sheep; man; and cattle. In our study; we show a synteny between EGF and the genes PDHA2 and FGF5 (from HSA 4q) and microsatell | 8978779 | [
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Synteny conservation between parts of human chromosome 4q and bovine and ovine chromosomes 6. | ites ILSTS018; ILSTS090; and ILSTS093 (from bovine chromosome 6) in sheep. We also show that the conservation between HSA 4q and sheep chromosome 6 is disrupted between EGF and FGF2. | 8978779 | [
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A complex satellite DNA polymorphism flanking the human ryanodine receptor gene (RYR1). | We describe a new highly polymorphic DNA marker flanking the human ryanodine receptor gene (RYR1) at chromosome band 19q13.1. The marker is composed of a 25bp minisatellite sequence; a compound microsatellite (AC)(AT); and an oligo-T stretch. STS mapping of previously published markers from 19q13.1 helped to integrate the genetic and physical maps of this region. Together with D19S422; the new polymorphism forms a pair of markers closely flanking either side of the RYR1 gene which may be useful for linkage | 8978780 | [
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A complex satellite DNA polymorphism flanking the human ryanodine receptor gene (RYR1). | studies in families susceptible to malignant hyperthermia and central core disease. | 8978780 | [
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Assignment of the human GATA4 gene to 8p23.1-->p22 using fluorescence in situ hybridization analysis. | Using fluorescence in situ hybridization; the human GATA4 gene; a member of the zinc-finger protein family recognizing the consensus GATA motif; was mapped to 8p23.1-->p22. This result; in conjunction with gene mapping data on other GATA-DNA binding proteins; suggests that genes encoding GATA-DNA binding proteins are dispersed throughout the genome; rather than clustered within a single locus. | 8978781 | [
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Effects of 5-azacytidine on the centromeric region of human fibroblasts studied by CREST staining and in situ hybridization on cytokinesis-blocked cells. | Several interphase methodologies have been applied in this study to investigate whether chemically induced undercondensation of the pericentromeric region of human fibroblast chromosomes promotes structural or numerical aberrations at the cell cycles following treatment. To achieve this aim; the effects of the hypomethylating agent 5-azacytidine (5- azaC) were studied on cytokinesis-blocked binucleated fibroblasts. This approach allowed the distribution of whole chromosomes or chromosome fragments in the da | 8978782 | [
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Effects of 5-azacytidine on the centromeric region of human fibroblasts studied by CREST staining and in situ hybridization on cytokinesis-blocked cells. | ughter nuclei and micronuclei of treated cells to be followed; since the daughter nuclei of a single mitosis are maintained in one cytoplasm by treatment with the actin inhibitor cytochalasin B. Antikinetochore staining and in situ hybridization with an alpha-satellite probe capable of detecting all human centromeres on 5-azaC-induced micronuclei in binucleated fibroblasts indicated that the predominant effect of the chemical is to induce micronuclei lacking centromeres; suggestive of induced chromosome abe | 8978782 | [
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Effects of 5-azacytidine on the centromeric region of human fibroblasts studied by CREST staining and in situ hybridization on cytokinesis-blocked cells. | rrations. Double in situ hybridization with alphoid and classical satellite DNA probes specific for chromosome 1 on binucleated fibroblasts was used to discriminate induced aneuploidy from breakage effects in the target area of hybridization. The results showed that undercondensation of the pericentromeric heterochromatin of human fibroblast chromosomes by treatment with 5-azaC produces structural chromosome aberrations involving the classical satellite DNA; whereas there was no evidence that the chemical i | 8978782 | [
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Effects of 5-azacytidine on the centromeric region of human fibroblasts studied by CREST staining and in situ hybridization on cytokinesis-blocked cells. | nduced chromosomal aneuploidy. | 8978782 | [
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Ordering of polymorphic markers in the chromosome region 3p21. | Starting from five markers; with a well-defined order from distal to proximal 3p21; nine other markers could be inserted in this 3p21 map. Five were precisely mapped genetically. The other markers were ordered by FISH and/or deletion hybrid mapping. The overall 3p21 order from distal to proximal is as follows: D3S1298-D3S1260-(D3S966; D3S1448 (= D3S1449)-D3S1029-D3S32-D3S643-D3F15S2 -D3S2968-D3S1235-D3S1289-D3S1447-D3S1295. | 8978783 | [
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The ETV6, CDKN1B and D12S178 loci are involved in a segment commonly deleted in various 12p aberration in different hematological malignancies. | Structural rearrangements including deletions of the short arm of chromosome 12 are frequent cytogenetic findings in various hematologic malignant disorders. Using FISH with a panel of DNA probes we detected loss of a common region of 12p in 22 patients with different hematologic disorders. Nine of them were characterized cytogenetically by a del(12p); seven by unbalanced translocations; and in the remaining cases the loss of the 12p region was masked by translocations and insertions; adding extra material | 8978784 | [
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The ETV6, CDKN1B and D12S178 loci are involved in a segment commonly deleted in various 12p aberration in different hematological malignancies. | to the short arm of chromosome 12. The smallest commonly deleted region found in all cases analyzed included ETV6; the gene for p27kipl (CDKN1B); and the D12S178 marker. | 8978784 | [
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Cloning, expression and chromosomal mapping of a novel cyclophilin-related gene (PPIL1) from human fetal brain. | We isolated a human cDNA clone encoding a novel protein homologous to cyclophilins; specific cellular targets of cyclosporin A; which are conserved in species ranging from human to prokaryotes. This cDNA; designated hCyPX; contained an open reading frame of 498 nucleotides encoding 166 amino acids. Computer analysis indicated that its predicted amino acid sequence had 41.6%; 40.4%; and 39.2% homology to those of human; bovine; and Drosophila cyclophilins; respectively. Northern blot analysis indicated ubiqu | 8978786 | [
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Cloning, expression and chromosomal mapping of a novel cyclophilin-related gene (PPIL1) from human fetal brain. | itous expression in adult human tissues; but most abundant expression in heart. Fluorescence in situ hybridization to human metaphase chromosomes localized this gene (PPIL1; peptidylprolyl isomerase [cyclophilin]-like 1) to chromosome bands 2p23.3-->p23.1. | 8978786 | [
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Calcineurin A alpha (PPP3CA), calcineurin A beta (PPP3CB) and calcineurin B (PPP3R1) are located on human chromosomes 4, 10q21-->q22 and 2p16-->p15 respectively. | Calcineurin (also called protein phosphatase-2B) is a calmodulin-regulated protein phosphatase which plays an important role in signal transduction. The enzyme is a heterodimer of a 58-59 kDa calmodulin-binding catalytic subunit (calcineurin A) and a small (i.e. 19 kDa) Ca(2+)-binding regulatory subunit (calcineurin B). The highly conserved calcineurin B is encoded by a single gene in all tissues except testes; whereas there are three isoforms of calcineurin A (alpha; beta and gamma) encoded by genes on thr | 8978785 | [
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Calcineurin A alpha (PPP3CA), calcineurin A beta (PPP3CB) and calcineurin B (PPP3R1) are located on human chromosomes 4, 10q21-->q22 and 2p16-->p15 respectively. | ee different chromosomes. This enzyme can play a critical role in transcriptional regulation and growth control in T lymphocytes by a mechanism believed to involve dephosphorylation of the nuclear factor NF-AT which is essential for transcription of the interleukin-2 gene. To better evaluate the potential role of the calcineurin genes in human genetic disorders; we have studied their chromosome locations. Calcineurin B (PPP3R1) is located on human chromosome 2p16-->p15 and calcineurin A beta (PPP3CB; previo | 8978785 | [
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Calcineurin A alpha (PPP3CA), calcineurin A beta (PPP3CB) and calcineurin B (PPP3R1) are located on human chromosomes 4, 10q21-->q22 and 2p16-->p15 respectively. | us gene symbol CALNB) is present on 10q21-->q22. We confirm the localization of calcineurin A alpha (PPP3CA; previous gene symbol CALNA) to chromosome 4 without regional localization. | 8978785 | [
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SOX20, a new member of the SOX gene family, is located on chromosome 17p13. | SOX genes share a high sequence identity with the HMG box present in the testis determining gene SRY. We have identified a HMG box-like sequence motif on six contiguous cosmids; which cross-hybridize to a SOX9 cDNA probe. A data base search revealed a high similarity of the deduced amino acid sequence to the human SOX12 and the murine Sox16 HMG domains. The cosmids were assigned to chromosome 17p13 by FISH analysis. | 8978787 | [
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Assignment of the human gene (GLCLR) that encodes the regulatory subunit of gamma-glutamylcysteine synthetase to chromosome 1p21. | We recently assigned the human gene (GLCLC) that encodes the catalytic subunit of gamma-glutamylcysteine synthetase (glutamate-cysteine ligase; E.C. 6.3.2.2) to human chromosome 6p12. Here we specify the chromosomal sublocalization of the human gene (GLCLR) that encodes the regulatory subunit of E.C. 6.3.2.2 to chromosome 1p21. | 8978789 | [
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[Allogeneic peripheral blood stem cell transplantation]. | Allogeneic peripheral blood stem cell transplantation (allo-PBSCT) has been increasingly used as an alternative to allogeneic bone marrow transplantation (allo-BMT). In comparison with allo-BMT; preliminary results indicate that rapid hematopoietic engraftment can be obtained; and there is no increase in the incidence and severity of acute GVHD after allo-PBSCT. Furthermore; general anesthesia is not required to collect a sufficient number of PBSCT; which are usually mobilized by G-CSF administration. There | 8978790 | [
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[Allogeneic peripheral blood stem cell transplantation]. | fore; allo-BMT will be replaced by allo-PBSCT in near future. | 8978790 | [
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[CMF or CAF combination chemotherapy for breast cancer]. | Cyclophosphamide is one of the alkylating agents and is a masked from compound. Combination chemotherapy with CMF or CAF has been commonly used as a first-line chemotherapy for recurrent and advanced breast cancer. The clinical efficacy of CMF chemotherapy (CPA + MTX + 5-FU) was evaluated on advanced and recurrent breast cancer. It is considered to be one of the most useful treatments. CAF chemotherapy (CPA + ADM + 5-FU) is also thought to be effective against breast cancers. | 8978791 | [
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[Effective clinical use of cyclophosphamide in hematological malignancies]. | Cyclophosphamide (CPA) is the most widely used alkylating agent in the treatment of hematological malignancies. We review current clinical potential of high-dose CPA administration in this field. The efficacy of dose intensification of CPA in CHOP regimen for patients with aggressive lymphoma is being investigated in some clinical trials. High-dose CPA administration and G-CSF infusion effectively mobilize high levels of peripheral blood stem cell suitable for autografting. High-dose regimens of CPA alone o | 8978792 | [
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[Effective clinical use of cyclophosphamide in hematological malignancies]. | r in combination with other agents also have been used to treat various hematological malignancies in conjunction with stem cell transplantation (SCT). A variety of adverse effects have been observed with the high-dose CPA used in preparative regimens for SCT. Hemorrhagic cystitis and cardiac toxicity are common and dose-limiting toxic effect of high-dose CPA administration. | 8978792 | [
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[Methotrexate in gynecologic oncology]. | Methotrexate produced the first remission in leukemia and the first cure of a solid tumor; choriocarcinoma. Methotrexate tightly binds to dihydrofolate reductase (DHFR); blocking the reduction of dihydrofolate to tetrahydrofolic acid; the active form of folic acid. Methotrexate also directly inhibits the folate-dependent enzymes of de novo purine and thymidylate synthesis. Resistance to methotrexate may develop as a result of elevated DHFR activity or defective transport of methotrexate into malignant cells | 8978793 | [
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[Methotrexate in gynecologic oncology]. | . Increased DHFR enzyme levels may also result from amplification of the DHFR gene; which is now clinically significant in selected patients. Methotrexate is an active drug in the first-line treatment of gestational trophoblastic disease (GTD) and in metastatic squamous cell carcinoma of the cervix. Since the introduction of methotrexate chemotherapy for malignant GTD; most hospitals have reported almost 100% cure rates for patients with nonmetastatic disease using single-agent regimens. Patients with low-r | 8978793 | [
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[Methotrexate in gynecologic oncology]. | isk metastatic disease have been treated with methotrexate and folinic acid and over 50% complete remission rates have been reported. Patients with metastatic GTD who had one or more high-risk factors benefited from initial multiagent chemotherapy; rather than waiting for acquisition of drug-resistance to single-agent therapy to start multiagent treatment. Using multiagent combination chemotherapy such as MAC (methotrexate; actinomycin D; cyclophosphamide) or EMA-CO (etoposide; methotrexate; actinomycin D a | 8978793 | [
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[Methotrexate in gynecologic oncology]. | nd cyclophosphamide; vincristine); most investigators have reported remission in approximately 60 to 80% of patients with high-risk metastatic GTD. Although the role of chemotherapy in carcinoma of the cervix has been limited for several reasons; trial of combination chemotherapy including methotrexate has been reported. However; it is still impossible to draw definite conclusions as to whether methotrexate combined with another clearly active drug may yield a superior response rate and survival. | 8978793 | [
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[Methotrexate-leucovorin rescue therapy: pharmacological characteristics and clinical effective use]. | An antifolate first introduced into the clinic about half decades ago; Methotrexate is effective against a variety of human cancers; when administered alone or in combination with leucovorin rescue at wide-ranging doses and by many different schedules. We know much more about MTX and its molecular and cellular pharmacology and mechanisms of anti-cancer action in light of current knowledge of the biochemistry of folate. This understanding has stimulated novel approaches to the clinical application of the dru | 8978794 | [
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[Methotrexate-leucovorin rescue therapy: pharmacological characteristics and clinical effective use]. | g and has greatly enhanced its therapeutic efficacy. | 8978794 | [
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[Sequential methotrexate and 5-fluorouracil]. | There are two purported biochemical mechanisms to explain the sequence-dependent synergy between methotrexate (MTX) and 5-fluorouracil (5-FU). One hypothesis is to increase incorporation of FU-TP into RNA and another is more sustained inhibition of DNA synthesis. Clinical studies on MTX.5-FU; significantly higher response rates and longer survival have been reported in gastric and colorectal cancer; especially high dose MTX.5-FU and adriamycin (FAMTX) in gastric cancer. An optimal schedule for treatment has | 8978795 | [
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[Sequential methotrexate and 5-fluorouracil]. | not yet been found. Reviewing data from the literature; a one-3 hour interval between the two drugs in gastric cancer and 3-8 hours in colorectal cancer seems to obtain better results. Sequential MTX.5-FU seems to be a very promising combination. | 8978795 | [
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[Adriamycin--breast cancer]. | The significance of doxorubicin (ADR) was reviewed in the treatment of advanced as well as early breast cancer. The history of ADR treatment; mainly as a combination of CAF; or FAC; has revealed that ADR is still the key drug for breast cancer; the response rate being 40-60%. A multivariate analysis in 182 advanced breast cancer cases showed that the predictive factor was the dominant site of metastasis; and performance status (PS). Overall survival was determined with the age; the dominant site of metastas | 8978796 | [
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[Adriamycin--breast cancer]. | is; and PS. Recently the combination chemotherapy including ADR was evaluated to be equivalent to CMF type chemotherapy in the adjuvant settings. | 8978796 | [
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[Characteristic and effective usage of adriamycin]. | The utility of adriamycin (doxorubicin); an anti-cancer antibiotic; for the treatment of transitional cell carcinoma of the bladder has been appreciated. Adriamycin is still used intravesically for superficial bladder cancer to avoid implantation of floating cancer cells after transurethral resection; to decrease the incidence of recurrence and treat an unresected part of the tumor. On the other hand; cisplatin-based multi-drug chemotherapies including adriamycin have proven effective in treating locally an | 8978797 | [
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[Characteristic and effective usage of adriamycin]. | d systemically advanced bladder cancer. | 8978797 | [
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[Etoposide in the treatment of small-cell lung cancer]. | Etoposide is one of the most active agents for small-cell lung cancer. It is usually administered intravenously over 3 to 5 consecutive days in combination with cisplatin; carboplatin; cyclophosphamide; or adriamycin. Recently; chronic daily administration of low dose oral etoposide and very high dose intravenous administration with autologous blood stem cell transplantation have been tried to improve treatment outcome. However; the standard dose; schedule; or route of etoposide administration have not been | 8978798 | [
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[Etoposide in the treatment of small-cell lung cancer]. | established. In this paper; the clinical efficacy and adverse effects of etoposide in the treatment of small-cell lung cancer are reviewed. | 8978798 | [
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[Etoposide (VP-16) in gynecologic malignancy]. | Clinical application of etoposide (VP-16) for gynecologic malignancy has been investigated in trophoblastic disease; ovarian; endometrial and cervical cancer. Etoposide has proved to be one of the most effective agents for trophoblastic disease. It is widely used in single or multiple drug regimens in chemotherapy of the disease. Furthermore; this drug is observed to be active in the combination of BLM with CDDP for germ cell tumor of the ovary. It is now playing an important role in chemotherapy of this tu | 8978799 | [
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[Etoposide (VP-16) in gynecologic malignancy]. | mor. | 8978799 | [
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[The role of etoposide therapy in urogenital cancer]. | Testicular cancer is recognized as a model of curable cancer by chemotherapy; and etoposide is the one of the most important drugs in its treatment. Etoposide has been used with cisplatin and bleomycin as a first-line combination chemotherapy since the early 1980s. It is now the key drug in the setting of high-dose chemotherapy for refractory cases. Oral; low-dose etoposide may provide effective palliation in some patients refractory to possible curative salvage therapy. Oral etoposide may also play a role | 8978800 | [
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[The role of etoposide therapy in urogenital cancer]. | in the maintenance therapy adjunctive to salvage therapy. Nevertheless; etoposide has failed to show definite therapeutic efficacy in the treatment of renal cell carcinoma and bladder cancer. The combination therapy of oral etoposide and estramustine; however; showed promising results for the treatment of hormone-refractory prostate cancer. Further investigations are required for this new treatment strategy for prostatic cancer. | 8978800 | [
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[Etoposide 21 therapy for malignant lymphoma]. | Oral administration of etoposide 50 mg daily for 21 days was studied in 83 patients with malignant lymphoma who were not indicated for intensive treatment. Dose escalation to 75 mg was allowed for previously untreated cases and those with body surface over 1.5 m2. The majority of patients were: median age 69; outpatient; previously treated; with clinical stage III; IV. Overall response rate was 52.5%; including 10 CR. Response rates for each background factor were: age > or = 65; 63.8%* < 65; 36.4%; PS 0; 5 | 8978801 | [
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[Etoposide 21 therapy for malignant lymphoma]. | 6.5%; 1; 53.9%; 2 approximately 3; 25.0%; outpatient 65.9%; inpatient 24.0%. Clinical stage I; II; 57.1%; III; IV; 50.0%. Dosage 50 mg 49.3%; 75 mg 85.7%. Previously untreated; 81.8%; pretreated; 47.8%*(*p < 0.05).moderate side effects such as loss of appetite; nausea and leukocytopenia were observed. Those were tolerable and recovery was achieved by reduction of dose or discontinuation. Intensive treatment is the most popular therapy for malignant lymphoma; however; there are certain patients not indicated | 8978801 | [
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[Etoposide 21 therapy for malignant lymphoma]. | for such intensive treatment. For such patients this etoposide 21 therapy can offer a new way of palliative therapy to allow them to remain at home while maintaining a high quality of life. | 8978801 | [
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[Bropirimine (U-54461S) phase I clinical studies]. | Bropirimine (U-54461S); an oral interferon (IFN) inducer that has also a direct antiproliferative activity; is a novel antitumor agent. To investigate the safety and pharmacokinetics of bropirimine tablets and to measure IFN concentrations in patients with cancer; Phase I studies were conducted. In single dose study (0.25 to 3g) and multiple-dose study with one-day dosing (1 or 2g; every one or two hours; three times a day); bropirimine treatment was well tolerated by the patients with cancer. In multiple-d | 8978802 | [
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[Bropirimine (U-54461S) phase I clinical studies]. | ose study with consecutive days dosing (1 or 2g; every 2 hours; three times a day for 3 or 5 consecutive days); a regimen with a dose of 1g orally administered every two hours; three times a day for three consecutive days was considered to be tolerable to cancer patients. Adverse drug reactions frequently observed were generalized malaise; fever; nausea/vomiting; anorexia; headache/dull headache; and tachycardia. Abnormalities in laboratory tests frequently observed were leukemia; neutropenia; thrombocytope | 8978802 | [
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[Bropirimine (U-54461S) phase I clinical studies]. | nia; and elevation in GOT/GPT. IFN was not induced in any patients in the single dose study. It was; however; induced in 3 of 16 cases (18.8%) in the one-day dosing study and in 6 of 7 cases (85.7%) in the consecutive days dosing study. As to clinical antitumor efficacy; a decrease in size of the tumor lesions and improvement in subjective/objective symptoms were noted in two cases in the one-day dosing study. With these findings; the regimen with the dose of 1g orally administered every two hours; three ti | 8978802 | [
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[Bropirimine (U-54461S) phase I clinical studies]. | mes a day for three consecutive days with a four-day drug-free interval per week was recommended for early phase II studies. | 8978802 | [
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[Administration of oral etoposide for one year as adjuvant chemotherapy for non-small cell lung cancer-side effect]. | The possibility of using etoposide 25 mg daily as adjuvant chemotherapy for non-small cell lung cancer was discussed. Initially; 73 patients who had undergone resection participated in this trial; 49 of them were eligible; 24 including a patient over 70 years of age (8) took the drug for 1 year; 22 patients resigned from the study due to side effects; and a further 3 patients died during the trial. The main side effects observed were gastro-intestinal trouble (13) and pneumonia (4). The side effects of this | 8978805 | [
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[Administration of oral etoposide for one year as adjuvant chemotherapy for non-small cell lung cancer-side effect]. | drug appeared early in the treatment phase. During the first month of treatment; 9 patients discontinued the drug due to side effects; and 10 more did so within 4 months. To achieve optimal conditions for the trial study of low-dose etoposide it was found important to select patients under 70 years of age and to control side effects of the drug on the gastro-intestinal tract from the beginning. | 8978805 | [
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[Chemotherapy for peritoneal dissemination in gastric cancer under ureteral catheterization]. | The treatment of peritoneal dissemination of gastric cancer is mainly chemotherapy; but it use is often limited by ileus; hydronephrosis and jaundice. We employed a ureteral catheter for 6 patients with hydronephrosis due to peritoneal dissemination. Chemotherapy (CDDP + ADM + 5-FU or MTX + 5-FU) was administered in 5 patients. After ureteral catheterization; renal function was kept within normal ranges; so chemotherapy was performed safely. One of five patients became CR and the effect of the treatment was | 8978803 | [
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[Chemotherapy for peritoneal dissemination in gastric cancer under ureteral catheterization]. | satisfactory (PR: 1; NC: 2). Thus; chemotherapy after ureteral catheterization may be effective for patients with peritoneal dissemination and hydronephrosis. | 8978803 | [
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[Significance of 5'-deoxy-5-fluorouridine (5'-DFUR) administration before surgery for advanced gastric and colonic cancers--activity of pyrimidine nucleoside phosphorylase (PyNPase) and serum immunosuppressive acidic protein (IAP)]. | We administered preoperatively 5'-deoxy-5-fluorouridine (5'-DFUR) for treatment of advanced gastric and colonic cancers; and measured pyrimidine nucleoside phosphorylase (PyNPase) in the excised tumor sample and serum immunosuppressive acidic protein (serum IAP); which is an index of the immunity of host-bearing cancer; while studying its direct antitumor effect and improved immunity. Patients with 24 advanced gastric cancers and 36 colonic cancers were randomly divided into a preoperatively administered gr | 8978804 | [
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[Significance of 5'-deoxy-5-fluorouridine (5'-DFUR) administration before surgery for advanced gastric and colonic cancers--activity of pyrimidine nucleoside phosphorylase (PyNPase) and serum immunosuppressive acidic protein (IAP)]. | oup and a non-administered group. In the preoperatively administered group; 5'-DFUR (1;200 mg/day) was orally administered on preoperative days 7 approximately 14. After collecting samples (about 0.5 g) from adjacent normal tissues with tumor within 30 minutes after extirpation of tumor and freezing those less than -20 degrees C; the PyNPase level was measured as soon as possible. Moreever; serum IAP levels at pre-administration in the administered group and on admission in the non-administered group were m | 8978804 | [
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[Significance of 5'-deoxy-5-fluorouridine (5'-DFUR) administration before surgery for advanced gastric and colonic cancers--activity of pyrimidine nucleoside phosphorylase (PyNPase) and serum immunosuppressive acidic protein (IAP)]. | easured. Those in the administered group were measured again on the operative day. No decreasing tendency of PyNPase was generally found in cases with gastric colonic cancers; and no significant difference in stage-II was not either. However; a decreasing tendency in tumor activity was found by pre-administration. Moreover; there was significant improvement in the serum IAP level in cases with gastric and colonic cancers by pre-operative administration of 5'-DFUR. This tendency was also found in advanced co | 8978804 | [
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[Significance of 5'-deoxy-5-fluorouridine (5'-DFUR) administration before surgery for advanced gastric and colonic cancers--activity of pyrimidine nucleoside phosphorylase (PyNPase) and serum immunosuppressive acidic protein (IAP)]. | lonic cancer with Dukes-C by Dukes's classification. In conclusion; it was suggested that the pre-operative administration of 5'-DFUR for treatment of advanced gastric and colonic cancers has a favorable influence for prognosis because the tumor region was retarded by the high PyNPase activity according to the severity of tumors and elevating tendency of the immune response in host. | 8978804 | [
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[Three cases of resected primary mediastinal yolk sac tumor following six courses of bleomycin, etoposide and cisplatin (BEP) combination chemotherapy]. | We experienced three cases of primary mediastinal yolk sac tumor which were resected after 6 courses of BEP chemotherapy with G-CSF support. All cases had high levels of AFP. CT scan revealed an anterior mediastinal tumor infiltrating the surrounding tissue in all cases; and multiple pulmonary nodules in one case. The serum AFP level decreased markedly; but did not return to normal after the chemotherapy was completed. The markedly decreased mediastinal tumor masses were removed with en-bloc resection of th | 8978807 | [
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[Three cases of resected primary mediastinal yolk sac tumor following six courses of bleomycin, etoposide and cisplatin (BEP) combination chemotherapy]. | e lung and pericardium. Viable tumor cells were not present in the resected tumors. These three cases remain free of disease at present; 3 years; 9 months and 5 months after operation. | 8978807 | [
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[Effect of FUT-187, oral serine protease inhibitor, on inflammation in the gastric remnant]. | Excessive enterogastric reflux following partial gastrectomy is believed to be responsible for the cause of inflammation in the gastric remnant. We examined the effect of FUT-187; a synthetic serine protease inhibitor; on symptoms and endoscopic findings in 33 patients who were diagnosed endoscopically as postgastrectomy gastritis. Patients took 50 mg FUT-187 orally after each meal and at bedtime for 8 weeks. Before treatment; 30 patients (91%) suffered from several symptoms including regurgitation and/or b | 8978806 | [
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[Effect of FUT-187, oral serine protease inhibitor, on inflammation in the gastric remnant]. | itter taste in the mouth (49%); epigastric pain (42%) and nausea (36%). From endoscopic observation; erythema was detected in 32 patients; edema in 23 patients and erosion and/or ulcer in 9 patients. After treatment the global improvement rating for subjective symptoms was 76.7% (23/30) and the improvement of endoscopic findings was 63.6% (21/33). Diarrhea was observed in one patient but could be easily controlled by discontinuation of the drug. Our results suggest that FUT-187 can be a useful drug for the | 8978806 | [
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[Effect of FUT-187, oral serine protease inhibitor, on inflammation in the gastric remnant]. | treatment of postgastrectomy gastritis with its efficacy and safety. | 8978806 | [
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[Two cases of relapse or refractory germ cell tumor who had been treated with combination chemotherapy of cisplatin and carboplatin]. | We tried a combination chemotherapy with cisplatin (CDDP) and carboplatin (CBDCA) (CDDP/CBDCA regimen) as salvage therapy for 2 cases with recurrent or refractory Germ Cell Tumor (GCT). Case 1 was a 29-year-old man with 2nd relapsed embryonal carcinoma and seminoma originating from testis. Case 2 was a 23-year-old man with primary refractory embryonal carcinoma and yolk sac tumor originating from mediastinum. CDDP and CBDCA were administered at the dose of 120 mg/m2 and 350 mg/m2 on day 1; and vinblastin wa | 8978809 | [
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[Two cases of relapse or refractory germ cell tumor who had been treated with combination chemotherapy of cisplatin and carboplatin]. | s administered at the dose of 10 mg/body on day 2. In one of two cases; a complete response was obtained. Non-hematologic toxicity of CDDP/CBDCA regimen was tolerable. It is suggested that this combination chemotherapy is useful for GCT recurrence. | 8978809 | [
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[Antitumor effect of TNP-470 against tongue carcinoma model by intratumor administration]. | nan | 8978810 | [
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[A case of recurrent squamous cell carcinoma of cervix successfully treated with BOMP]. | Combination chemotherapy consisting of bleomycin; vincristine; mitomycin C and cisplatin (BOMP) was applied to a case (57 years old) with recurrence of squamous cell carcinoma of the cervix. The patient with FIGO stage IIIb cervical carcinoma had been treated with radiotherapy seven years ago. She was affected by malignant pericardial effusion; and was treated with direct intrapericardial administration of cisplatin (20 mg). After instillation; ultrasonography and computed tomography showed the wall thicken | 8978808 | [
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[A case of recurrent squamous cell carcinoma of cervix successfully treated with BOMP]. | ing of colon and marked ascites. Barium enema showed the circumferential narrowing and serrations of colon; and it was diagnosed to be carcinomatous peritonitis. She was treated with BOMP consisting of BLM (5 mg; i.m.; days 1 - 5); VCR (1 mg; i.v.; day 5'); MMC (8 mg; i.v.; day 5); and CDDP (70 mg; d.i.v.; day 5). After three courses of BOMP therapy; she achieved a complete response with few toxic effects and did not require special care. She has been free of disease for six months and her performance statu | 8978808 | [
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[A case of recurrent squamous cell carcinoma of cervix successfully treated with BOMP]. | s is 0. This encouraging result warrants the use of this combination chemotherapy regimen in other patients with recurrent squamous cell carcinoma of the cervix. | 8978808 | [
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[A study of combination chemotherapy using, 5-FU and CDDP in patients with advanced non-small cell lung cancer]. | nan | 8978811 | [
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[Antimetastatic effects of UFT on murine liver metastasis model using L5178YHM lymphoma]. | nan | 8978812 | [
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Three-dimensional reconstruction of painted human interphase chromosomes: active and inactive X chromosome territories have similar volumes but differ in shape and surface structure. | This study provides a three-dimensional (3D) analysis of differences between the 3D morphology of active and inactive human X interphase chromosomes (Xa and Xi territories). Chromosome territories were painted in formaldehyde-fixed; three-dimensionally intact human diploid female amniotic fluid cell nuclei (46; XX) with X-specific whole chromosome compositive probes. The colocalization of a 4;6-diamidino-2-phenylindole dihydrochloride-stained Barr body with one of the two painted X territories allowed the u | 8978813 | [
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Three-dimensional reconstruction of painted human interphase chromosomes: active and inactive X chromosome territories have similar volumes but differ in shape and surface structure. | nequivocal discrimination of the inactive X from its active counterpart. Light optical serial sections were obtained with a confocal laser scanning microscope. 3D-reconstructed Xa territories revealed a flatter shape and exhibited a larger and more irregular surface when compared to the apparently smoother surface and rounder shape of Xi territories. The relationship between territory surface and volume was quantified by the determination of a dimensionless roundness factor (RF). RF and surface area measure | 8978813 | [
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Three-dimensional reconstruction of painted human interphase chromosomes: active and inactive X chromosome territories have similar volumes but differ in shape and surface structure. | ments showed a highly significant difference between Xa and Xi territories (P < 0.001) in contrast to volume differences (P > 0.1). For comparison with an autosome of similar DNA content; chromosome 7 territories were additionally painted. The 3D morphology of the chromosome 7 territories was similar to the Xa territory but differed strongly from the Xi territory with respect to RF and surface area (P < 0.001). | 8978813 | [
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A novel ubiquitin-like modification modulates the partitioning of the Ran-GTPase-activating protein RanGAP1 between the cytosol and the nuclear pore complex. | Ran is a nuclear Ras-like GTPase that is required for the bidirectional transport of proteins and ribnucleoproteins across the nuclear pore complex (NPC). A key regulator of the Ran GTP/GDP cycle is the 70-kD Ran-GTPase-activating protein RanGAP1. Here; we report the identification and localization of a novel form of RanGAP1. Using peptide sequence analysis and specific mAbs; RanGAP1 was found to be modified by conjugation to a ubiquitin-like protein. Immunoblot analysis and immunolocalization by light and | 8978815 | [
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A novel ubiquitin-like modification modulates the partitioning of the Ran-GTPase-activating protein RanGAP1 between the cytosol and the nuclear pore complex. | EM demonstrated that the 70-kD unmodified from of RanGAP1 is exclusively cytoplasmic; whereas the 90-kD modified form of RanGAP1 is associated with the cytoplasmic fibers of the NPC. The modified form of RanGAP1 also appeared to associated with the mitotic spindle apparatus during mitosis. These findings have specific implications for Ran function and broad implications for protein regulation by ubiquitin-like modifications. Moreover; the variety and function of ubiquitin-like protein modifications in the c | 8978815 | [
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A novel ubiquitin-like modification modulates the partitioning of the Ran-GTPase-activating protein RanGAP1 between the cytosol and the nuclear pore complex. | ell may be more diverse than previously realized. | 8978815 | [
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Lamin proteolysis facilitates nuclear events during apoptosis. | Expression of the adenovirus E1A oncogene stimulates both cell proliferation and p53-dependent apoptosis in rodent cells. p53 implements apoptosis in all or in part through transcriptional activation of bax; the product of which promotes cell death. The adenovirus E1B 19K product is homologous in sequence and in function to Bcl-2; both of which bind to and inhibit the activity of Bax and thereby suppress apoptosis. The E1B 19K protein also interacts with the nuclear lamins; but the role of this interaction | 8978814 | [
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Lamin proteolysis facilitates nuclear events during apoptosis. | in the regulation of apoptosis is not known. Lamins are; however; substrates for members of the interleukin-1 beta-converting enzyme (ICE) family of cysteine proteases that are activated during apoptosis and function downstream of Bcl-2 in the cell death pathway. lamins are degraded during E1A-induced p53-dependent apoptosis. Lamin A and C are cleaved into 47- and 37-kD fragments; respectively; and the site of proteolysis is mapped to a conserved aspartic acid residue at position 230. The cleavage of lamins | 8978814 | [
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