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Corneal change accompanying orthokeratology. Plastic or elastic? Results of a randomized controlled clinical trial. | Effects of orthokeratology on refractive error; visual acuity; and corneal curvature were monitored on two randomized comparison groups for 364 days of lens wear and 95 days of follow-up after lens wear was discontinued. Approximately 36% of the treatment group compared with 13% of the control group had 1 diopter or more change in refractive error; however; after lens wear was discontinued; there was substantial remission and differences between the groups were small. Although there was a positive correlati | 6360111 | [
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Corneal change accompanying orthokeratology. Plastic or elastic? Results of a randomized controlled clinical trial. | on between the amount of change during lens wear and the persistence of change after discontinuation; neither the magnitude of persistence nor differences between groups were clinically important. The lack of persistence indicates that the cornea is either highly elastic or has some other memory mechanism. We conclude that orthokeratology produces modest reductions in myopia; however; the effect will not persist without continued lens wear and therefore is of limited clinical value in permanently reducing m | 6360111 | [
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Corneal change accompanying orthokeratology. Plastic or elastic? Results of a randomized controlled clinical trial. | yopia. | 6360111 | [
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Descemet's membrane in penetrating keratoplasties of the human eye. | The response of Descemet's membrane in penetrating keratoplasty wounds was studied by light microscopy in 23 human eyes. Although only parts of the grafted areas could be sectioned in each eye; 15 splittings; full-thickness flanges; or fragmentations of Descemet's membrane were found in ten specimens. Seven of these abnormalities resulted in incarceration of Descemet's membrane into the wound. Malapposition of the wound edges was a causative factor in two additional eyes. Six of these incarcerations produce | 6360112 | [
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Descemet's membrane in penetrating keratoplasties of the human eye. | d a visible stromal wound defect. Exacting surgical apposition of the stromal edges of the graft and recipient cornea can therefore be expected to reduce; but not eliminate; these healing abnormalities. Great care must be taken in cutting the graft and removing the recipient tissue to avoid stripping the tough Descemet's membrane from the overlying softer stroma with dull instruments or overlapping cuts. | 6360112 | [
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Epididymal functions and their hormonal regulation. | The epididymis is a complex organ which maintains a specific intraluminal environment thought to be important for effecting sperm maturation in proximal regions and sperm storage in distal regions of the duct. The composition of the internal milieu is achieved both by transport between blood and lumen (and vice versa) and by synthesis and secretion into the lumen. Several low-molecular weight organic molecules achieve high concentration in the epididymal lumen; but their functions in the events of sperm mat | 6360113 | [
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Epididymal functions and their hormonal regulation. | uration and storage still remain unclear. Metabolic processes occurring within epididymal tissue and the absorptive and secretory activity of the epididymal epithelium are regulated by androgens. The synthesis of some; but not all; secretory proteins is also androgen-dependent. In addition to androgens; other hormones and local testicular factors may influence epididymal function. There is now increasing evidence that epididymal-specific and androgen-dependent secretory proteins play a fundamental role in m | 6360113 | [
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Growth and gliotic response of astrocytes in vitro. | We investigated the development of astrocytes in mechanically dissociated primary cultures of foetal and neonatal rat brain grown in different culture media using immunofluorescence and antiserum to glial fibrillary acidic protein (GFAP). GFAP-positive cells developed at a time corresponding to the 16th day of embryonic development and initially grew more slowly in Dulbecco's modified Eagle's Medium (DMEM) than in Medium 199 (M199) or Modified Eagle's Medium (MEM); but as the cultures matured a greater prop | 6360114 | [
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Growth and gliotic response of astrocytes in vitro. | ortion of GFAP-positive cells was obtained in DMEM; resulting in relatively pure populations of GFAP-positive cells after 1-2 months in vitro. GFAP-positive cells comprised process-bearing and fibroblastoid cell types and cells with intermediate morphology. Cultures in DMEM were also characterized by the appearance after 8-18 days in vitro of thick long bands of glial processes surrounding cyst-like spaces. The morphological change may represent a gliotic reaction to necrosis in vitro. | 6360114 | [
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An introduction to clinical decision analysis: bone marrow transplantation for aplastic anemia. | Clinical decision analysis is a systematic and rigorously logical approach to medical decision making under conditions of uncertainty. The structuring of a clinical problem in a decision analytic framework enables the clinician to focus on one aspect of a complex decision at a time without losing sight of the whole. The concepts of clinical decision analysis are explained and the technique is applied to compare bone marrow transplantation with supportive care for the treatment of a patient with severe aplas | 6360115 | [
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An introduction to clinical decision analysis: bone marrow transplantation for aplastic anemia. | tic anemia. The advantages and disadvantages of clinical decision analysis are discussed. The technique is intended to complement; but not replace; clinical judgement. | 6360115 | [
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Aspiration pneumonia in a wild platypus Ornithorhynchus anatinus. | nan | 6360119 | [
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Torsade de pointes and asystole in a child with complete heart block and prolonged QT interval. | Three-year-old girl with eight month history of convulsive syncope due to complete heart block; prolonged QT interval and paroxysms of ventricular tachycardia is described. An episode of torsade de pointes tachycardia followed by asystole was documented. The symptoms and electrocardiographic abnormalities were controlled by pacemaker therapy. The subset of "congenital heart block" patients with prolonged QT interval is at high risk of sudden death and should be regarded as a previously unrecognized form of | 6360118 | [
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Torsade de pointes and asystole in a child with complete heart block and prolonged QT interval. | Romano-Ward syndrome. | 6360118 | [
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Continuous ambulatory peritoneal dialysis (CAPD): an established treatment for endstage renal failure. | This paper is a study of 117 patients with endstage renal failure; treated by continuous ambulatory peritoneal dialysis (CAPD) over periods of 1-56 months. The study has shown CAPD to be an effective form of dialysis with a number of advantages over intermittent peritoneal dialysis and hemodialysis (better control of salt and water status; hypertension and anemia; steady state biochemistry and greater ease of self-dialysis). Peritoneal clearance and ultrafiltration have remained adequate in all but a few pa | 6360116 | [
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Continuous ambulatory peritoneal dialysis (CAPD): an established treatment for endstage renal failure. | tients. Hypoproteinemia; poor nutrition; obesity and abdominal herniae have been problems in a small percentage of patients. Hyperlipidemia has developed in half the patients but improved with diet. Peritonitis remains the major barrier to the more widespread use of CAPD; although its incidence can be considerably reduced by use of better connectors; bacterial filters and choice of patients. | 6360116 | [
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Diabetic autonomic neuropathy in children: immediate heart-rate response to standing. | The immediate heart-rate response to standing was used to investigate possible autonomic neuropathy in a group of 38 insulin-dependent diabetic patients in the paediatric age group. The controls and most of the diabetics showed the characteristic response to standing of a rapid increase in heart-rate and a fall to the resting rate within 15 seconds. Some diabetics showed a flat response or a prolonged gradual increase of heart-rate on standing. The usual method of describing the change in heart rate that oc | 6360117 | [
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Diabetic autonomic neuropathy in children: immediate heart-rate response to standing. | curs in response to standing is to compare the R-R intervals at the 30th and 15th beats (30:15 ratio). This is considered less appropriate in children because of the wider variability in heart rate due to sinus arrhythmia. An alternative ratio; the "maximum R-R/minimum R-R ratio" is proposed. This ratio is calculated by dividing the length of the maximum R-R interval (which occurred after the minimum R-R interval during the 30 second recording) by the length of the minimum R-R interval. Compared to 38 index | 6360117 | [
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Diabetic autonomic neuropathy in children: immediate heart-rate response to standing. | -selected age and sex matched controls; the diabetics showed a significantly lower mean "maximum R-R/minimum R-R ratio (P = 0.033). Six of the diabetics had results lower than the lowest control result. This test gives a prevalence rate of changes suggestive of diabetic autonomic neuropathy of approximately 15% in this age group. | 6360117 | [
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What's the thing to do when you're feeling depressed? A cross-cultural replication. | nan | 6360123 | [
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What makes depressed people feel worse? A cross-cultural replication. | nan | 6360124 | [
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Evaluation of the heterophil/lymphocyte ratio as a measure of stress in chickens. | The number of lymphocytes in chicken blood samples decreased and the number of heterophils increased in response to stressors and to increasing levels of corticosterone in the chicken feed. The ratio of heterophils to lymphocytes was less variable than the number of heterophil or lymphocyte cells; and the range of values for this ratio was greater than the range of values for heterophils and lymphocytes among control and experimental groups. The heterophil/lymphocyte ratio appears to be a more reliable indi | 6360120 | [
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Evaluation of the heterophil/lymphocyte ratio as a measure of stress in chickens. | cator of levels of corticosterone in the feed and to social stress than were the plasma corticosteroid levels. | 6360120 | [
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Neuroticism and the recall of positive and negative personality information. | nan | 6360126 | [
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Treatment of adolescent agoraphobics: effects on parent-adolescent relations. | nan | 6360127 | [
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The effects of flooding on reducing snake fear in rhesus monkeys: 6-month follow-up and further flooding. | nan | 6360128 | [
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Self-reinforcement: appealing misnomer or effective mechanism? | nan | 6360131 | [
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A comparative outcome study of behavioral social-skills training, interpersonal-problem-solving and non-directive control treatments with child psychiatric outpatients. | nan | 6360130 | [
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A preliminary report on the nature of interactions in a mental-handicap institution. | nan | 6360134 | [
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Nucleotype and cell size in vertebrates: a review. | The relationships between genome size and various cell morphometric parameters have been assayed in 357 species of Vertebrates; in order to verify the existence and significance of the so-called "nucleotypic effect" in this subphylum. The results obtained clearly manifest a significant relationship between the increase in genome size and that in nuclear volume; nuclear surface; cell volume and cell surface. A precise correlation is also observed between the increase in DNA content and the decrease in the su | 6360135 | [
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Nucleotype and cell size in vertebrates: a review. | rface/volume ratios of the nucleus and the cell. Other parameters; such as the nucleoplasmic index and DNA concentration; though showing a slight increase with increasing genome size; have values rather homogeneous in each Vertebrate group. These results have allowed some interesting speculations on various problems; for example; the mechanisms through which genome size can influence the cell size; the influence of the DNA content and cell morphometric parameters on functional level of the cell and the orga | 6360135 | [
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Nucleotype and cell size in vertebrates: a review. | nism; the importance of the nucleotypic effect in the adaptation to the environment of the various Vertebrate groups. From this study it seems possible to make the following conclusions: 1) in Vertebrates; genome size would exert a real nucleotypic influence on cell size; 2) genome sizes and cell morphometric parameters seem to be involved in the regulation of cell metabolism; 3) the regulation of some morphometric parameters depends strictly and automatically on the DNA amount or on other morphometric para | 6360135 | [
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Nucleotype and cell size in vertebrates: a review. | meters. The regulation of others; instead; depends on the interaction of different factors; which do not always act synergically; 4) the nucleotypic effect seems to have different distribution and importance in Anamniotes and Amniotes. | 6360135 | [
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Cell subpopulations in the paracortical area of the normal human lymphnode defined by monoclonal antibodies. | The cell subpopulations of normal human lymphnode were studied by immunohistochemical and immunoelectronmicroscopy techniques carried out by using monoclonal antibodies. OKT3 positive cells were demonstrated overwhelming in comparison to both OKT4 positive cells and OKT8 positive cells; furthermore la positive cells; dendritic in shape; were observed. Our results; compared to literature data; confirm that paracortex of limphnode is a T-dependent area. Moreover a possible role of the la positive dendritic ce | 6360136 | [
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Cell subpopulations in the paracortical area of the normal human lymphnode defined by monoclonal antibodies. | lls in the immunological education of T helper-inducer lymphocytes was hypotised. Finally; our immunoelectronmicroscopic findings prove that OKT4 positive cells and OKT8 positive cells show different ultrastructural patterns. | 6360136 | [
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Haemopoietic tissue in Perinereis cultrifera Grübe: cytological identification and observations after treatment with lead. | A perivascular tissue made up of two different types of cells (one with a slightly basophilic cytoplasm and many Fe+++ ions; the other with haemopigments) has been demonstrated in Perinereis cultrifera Grübe by means of histochemical and cytochemical techniques. These two types of cells probably cooperate in the production of the respiratory pigment. Treatment with lead nitrate (0.02 ppm) for up to a maximum of five days caused a progressive cytological alteration with inhibition of haemoglobin synthesis. | 6360138 | [
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Impermeability of newt cerebral and pial capillaries to exogenous peroxidase. A light and electron microscope study. | The permeability of cerebral vessels to exogenous peroxidase was studied in the newt. The reaction product was found only inside the cerebral or pial blood vessels. Electron microscope investigations revealed the presence of reaction product along the luminal area of vessels and in some parts of the intercellular spaces at the level of tight junctions joining endothelial cells. On the basis of the ultrastructural peroxidase localization; the presence of a brain-blood barrier in Triturus is discussed. | 6360137 | [
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Cytochemical identification of the leukocytes of torpedoes (Torpedo marmorata Risso and Torpedo ocellata Rafinisque). | The authors subjected peripheral blood smears of Torpedoes to cytochemical analysis of lipids; protein; neutral and acid polysaccahrides and of some enzymatic activities; i.e. adenosine triphosphatase (ATP-ase); acid and alkaline phosphatase; aliesterase and peroxidase. It was found that neutrophilic granulocytes are intensely PAS and aliesterase positive and weakly ATP-ase positive. Eosinophilic granulocytes show the presence of neutral polysaccharides in the matrix (which is PAS positive) and strong ATP-a | 6360139 | [
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Cytochemical identification of the leukocytes of torpedoes (Torpedo marmorata Risso and Torpedo ocellata Rafinisque). | se and acid phosphatase activities in the granules. Lymphocytes sometimes contain weakly PAS and aliesterase positive granules. Monocytes show some small PAS positive granules and weak acid phosphatase and aliesterase activities. Thrombocytes contain some peripheral granules which are PAS positive and slightly ATP-ase positive. There are no transitional forms between the various cellular types. The results confirm the classification of leukocytes of Torpedoes into neutrophilic granulocytes; eosinophilic gra | 6360139 | [
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Cytochemical identification of the leukocytes of torpedoes (Torpedo marmorata Risso and Torpedo ocellata Rafinisque). | nulocytes; lymphocytes; monocytes and thrombocytes and contribute some informations about the histoenzymatic content of Elasmobranch leukocytes. | 6360139 | [
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Interactions between coronary occlusion and the renin-angiotensin system in the dog. | Studies were carried out in 39 barbiturate-anesthetized dogs to determine whether the renin-angiotensin system is important in control of hemodynamics and coronary flow during myocardial ischemia. Plasma renin activity (PRA) was 2.2 +/- 0.4 ng x ml-l x hr-1 immediately before coronary artery occlusion (CAO) and increased to 3.8 +/- 0.5 (p less than .005) 15 minutes after CAO. In nephrectomized dogs; PRA was 0.76 +/- 0.14 ng x ml-l x hr-1 two hours after nephrectomy and remained unchanged after CAO. In contr | 6360140 | [
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Interactions between coronary occlusion and the renin-angiotensin system in the dog. | ast; hemodynamic changes following CAO were similar between nephrectomized and intact dogs: mean arterial pressure fell from 126 +/- 4 pre CAO to 116 +/- 4 mm Hg post CAO (p less than 0.005) in nephrectomized dogs and from 130 +/- 11 to 120 +/- 11 mm Hg (p less than 0.005) in intact dogs. Left atrial pressure rose from 5.4 +/- 0.9 pre CAO to 7.7 +/- 0.9 mm Hg (p less than 0.005) post CAO in nephrectomized from 5.4 +/- 0.9 pre CAO to 7.7 +/- 0.9 mm Hg (p less than 0.005) post CAO in nephrectomized dogs and 6 | 6360140 | [
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Interactions between coronary occlusion and the renin-angiotensin system in the dog. | .3 +/- 1.3 to 9.0 +/- 1.8 mm Hg (p less than 0.005) in intact dogs. Heart rate remained unchanged in both groups. In sham-operated dogs without CAO; neither the angiotensin II blocker Saralasin nor the converting enzyme inhibitor Captopril had significant effects on systemic (SVR) and coronary (CVR) vascular resistances. In contrast; in dogs with CAO; these drugs reduced CVR from 1.28 +/- 0.13 mm Hg x ml-1 x min x 100 g heart weight (resistance units = RU) to 0.85 +/- 0.08 RU (p less than 0.05) (Saralasin) | 6360140 | [
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Interactions between coronary occlusion and the renin-angiotensin system in the dog. | 15 minutes after treatment and from 1.17 +/- 0.09 to 0.88 +/- 0.08 RU (p less than 0.025); (Captopril) respectively. However; only Captopril reduced SVR; from 10.7 +/- 1.13 to 8.2 +/- 0.8 RU (p less than 0.025). Both Captopril and Saralasin induced a significant increase in collateral blood flow. Nephrectomy; two hours prior to CAO; significant increase in collateral blood flow. Nephrectomy; two hours prior to CAO; significantly reduced the effect of Captopril on CVR and collateral blood flow while the effe | 6360140 | [
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Interactions between coronary occlusion and the renin-angiotensin system in the dog. | ct on SVR persisted. Thus the reduction in CVR appears to be an effect of inhibition of the renin-angiotensin system; this system participates in control of CVR during CAO and may limit coronary collateral blood flow. | 6360140 | [
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Benzyl p-guanidinothiobenzoate hydrochloride, a new active-site titrant for trypsin and trypsin-like enzymes. | Benzyl p-guanidinothiobenzoate hydrochloride was synthesized and demonstrated to be useful for active-site titration of bovine trypsin; bovine thrombin; human lung tryptase; bovine activated protein C; human Factor XIIa fragment and bovine Factor Xa beta. The titration is based on rapid formation of a stable acyl-enzyme with a stoichiometric release of benzyl thiol. Thiol production is measured quantitatively by including 4;4'-dithiodipyridine in the reaction mixture and measuring the increase in absorbance | 6360155 | [
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Benzyl p-guanidinothiobenzoate hydrochloride, a new active-site titrant for trypsin and trypsin-like enzymes. | at 324 nm. Ellman's reagent has also been successfully employed; allowing measurement at 410 nm. Unlike p-nitrophenyl p'-guanidinobenzoate; the thioester titrant reacts slowly with chymotrypsin A alpha thus eliminating interference by this enzyme in most titrations. Advantages of this reagent as a titrant include: flexibility in detection of the released thiol; selectivity between trypsin and chymotrypsin-like enzymes; minimal pH-dependence of the epsilon of the absorbing species; relative stability of the | 6360155 | [
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Benzyl p-guanidinothiobenzoate hydrochloride, a new active-site titrant for trypsin and trypsin-like enzymes. | reagent under titration conditions; and high epsilon at pH 7.2 with either 4;4'-dithiodipyridine or Ellman's reagent. The reagent should prove useful as an alternative to p-nitrophenyl p'-guanidinobenzoate hydrochloride for the determination of active-site concentrations of the enzymes employed; as well as of other related enzymes. | 6360155 | [
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The effect of membrane composition and alcohols on the insulin-sensitive reconstituted monosaccharide-transport system of rat adipocyte plasma membranes. | The monosaccharide transporter from the plasma membranes of rat adipocytes and insulin-stimulated adipocytes has been reconstituted in sonicated liposomes. The stereospecific D-glucose uptake by liposomes made from a range of phospholipids and incorporating fatty acids has been investigated. D-Glucose uptake is correlated with an increase in lipid fluidity as a consequence of the addition of fluidizing fatty acids; changes in phospholipid acyl chain length and temperature. Benzyl alcohol and ethyl alcohol; | 6360157 | [
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The effect of membrane composition and alcohols on the insulin-sensitive reconstituted monosaccharide-transport system of rat adipocyte plasma membranes. | which are generally considered to increase bilayer fluidity; decrease stereo-specific D-glucose uptake in both whole adipocytes and reconstituted liposomes. It is suggested that; although these alcohols may affect D-glucose transport by lipid-mediated fluidity changes; they also interact directly with the transporter resulting in inhibition of transport. | 6360157 | [
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Identification of cell surface dipeptidylpeptidase IV in human fibroblasts. | An antigen with dipeptidylpeptidase IV activity was identified at the surface of normal human fibroblasts. Hydrophobic interaction electrophoresis in phenyl-Sepharose revealed that the enzyme contained a hydrophobic domain; while lactoperoxidase-catalysed iodination with 125I of living cells indicated that the protein was located at the cell surface. Crossed immunoelectrophoresis with specific antibodies of acid-extracted or papain-treated cells showed a shift of the dipeptidylpeptidase IV peak to a faster | 6360158 | [
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Identification of cell surface dipeptidylpeptidase IV in human fibroblasts. | mobility. The molecular properties of the fibroblast enzyme were clearly different from those described for dipeptidylpeptidase IV from other tissues and species. Fibroblast dipeptidylpeptidase IV contained two different disulphide-linked subunits; of apparent Mr values 125000 and 135000 (denatured and reduced). In gel filtration; an Mr of about 400000 was observed for the unreduced molecule. The enzymic properties of fibroblast dipeptidylpeptidase IV were very similar to those of the well-characterized pig | 6360158 | [
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Identification of cell surface dipeptidylpeptidase IV in human fibroblasts. | kidney enzyme. Activity towards glycyl-L-prolyl-beta-naphthylamide was inhibited 50% by 0.023 mM-di-isopropylphosphorofluoridate. L-Alanyl-L-alanyl-beta-naphthylamide was hydrolysed ten times more slowly than glycyl-L-prolyl-beta-naphthylamide. | 6360158 | [
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Studies on human gastric mucosal zymogen granules and their zymogens. | Intact human gastric mucosal zymogen granules (ZG) were detected in specimens from surgical resections of one patient with gastric adenocarcinoma and two with benign gastric ulcer. Both large ZG with unilaminar membranes and smaller ZG with trilaminar membranes were identified by electron microscopy. The zymogens in the ZG and in mucosal extracts were separated by gel electrophoresis. Slow-moving Protease (SMP) was seen in the whole mucosal extracts but was absent from ZG. One specimen of pyloric mucosa sho | 6360160 | [
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Studies on human gastric mucosal zymogen granules and their zymogens. | wed a striking absence of ZG. Despite the absence of ZG; pyloric mucosa showed Pepsinogens 2-5 (constituents of PG I) as well as Pepsinogens 6 and 7 (constituents of PG II) and SMP. | 6360160 | [
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Assay of glyoxalase I in blood. | Glyoxalase I (S-lactoyl-glutathione methylglyoxal-lyase (isomerizing); EC 4.4.1.5) was assayed using alcoholic; acidic 2;4-dinitrophenylhydrazine to follow the disappearance of methylglyoxal over time; with the absorbance of formed methylglyoxal bis-hydrazone measured at 432 nm. Erythrocyte glyoxalase I activities were found to be 64; 41; and 18 mumole of S-lactoyl glutathione formed min-1 X ml-1 of red blood cells in rat; human; and rabbit blood and 174 mumole X min-1 X mg-1 of protein for yeast. The Km va | 6360161 | [
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Assay of glyoxalase I in blood. | lues found in millimolar hemimercaptal were about 0.5. Glyoxalase I activity can be determined in crude tissue preparations without interference from biological materials. | 6360161 | [
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Characterization of succinate dehydrogenase and alpha-glycerophosphate dehydrogenase in pancreatic islets. | Succinate dehydrogenase activities in homogenates of rat and ob/ob mouse pancreatic islets were only 13% of the activities in homogenates of liver and were also several times lower than in homogenates of pancreatic acinar tissue. This indicates that the content of mitochondria in pancreatic islet cells is very low. The very low activity of succinate dehydrogenase is in agreement with the low mitochondrial volume in the cytoplasmic ground substance of pancreatic islet cells as observed in morphometric studie | 6360162 | [
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Characterization of succinate dehydrogenase and alpha-glycerophosphate dehydrogenase in pancreatic islets. | s. This may represent the poor equipment of pancreatic islet cells with electron transport chains and thus provide a regulatory role for the generation of reducing equivalents and chemical energy for the regulation of insulin secretion. The activities of succinate dehydrogenase in tissue homogenates of pancreatic islets; pancreatic acinar tissue; and liver were significantly inhibited by malonate and diazoxide but not by glucose; mannoheptulose; streptozotocin; or verapamil. Tolbutamide inhibited only pancr | 6360162 | [
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Characterization of succinate dehydrogenase and alpha-glycerophosphate dehydrogenase in pancreatic islets. | eatic islet succinate dehydrogenase significantly; providing evidence for a different behavior of pancreatic islet cell mitochondria. Therefore diazoxide and tolbutamide may affect pancreatic islet function through their effects on succinate dehydrogenase activity. The activities of alpha-glycerophosphate dehydrogenase in homogenates of pancreatic islets and liver from rats and ob/ob mice were in the same range; while activities in homogenates of pancreatic acinar tissue were lower. None of the test agents | 6360162 | [
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Characterization of succinate dehydrogenase and alpha-glycerophosphate dehydrogenase in pancreatic islets. | affected alpha-glycerophosphate dehydrogenase activity. Thus the results provide no support for the recent contention that alpha-glycerophosphate dehydrogenase activity may be critical for the regulation of insulin secretion. | 6360162 | [
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Proteolytic activities in plasma membrane preparations from rat liver. 1. Preparation of rat liver plasma membranes and solubilization of membrane bound proteases. | Plasma membranes (PM) were prepared by discontinuous density gradient centrifugation of crude nuclear fractions from 6 rat livers. These "nuclear" PM (PM-n) were 15-fold enriched in plasma membrane marker enzymes and contained an endopeptidase activity degrading azocasein at pH 7. To get larger amounts of plasma membranes; microsomal fractions obtained in large scale subcellular fractionations were subjected to continuous gradient zonal centrifugation. These "microsomal" PM (PM-m) were 22-fold enriched in 5 | 6360163 | [
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Proteolytic activities in plasma membrane preparations from rat liver. 1. Preparation of rat liver plasma membranes and solubilization of membrane bound proteases. | '-nucleotidase; however; the separation of PM from endocellular membranes was not complete. PM-m showed endopeptidase activity degrading azocasein at pH 5.4 faster than at pH 7.5 and exopeptidases degrading Ala-Pro-pNA and Ala-pNA at pH 7.6. The latter two activities were distributed over the gradient similar to PM marker enzymes and can be solubilized by detergent and proteinase treatment. Therefore; dipeptidyl-aminopeptidase IV and Ala-aminopeptidase are intrinsic plasma membrane enzymes and can be used a | 6360163 | [
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Proteolytic activities in plasma membrane preparations from rat liver. 1. Preparation of rat liver plasma membranes and solubilization of membrane bound proteases. | s additional markers for rat liver plasma membranes. The efficiency and selectivity to solubilize plasma membrane bound endopeptidase; DPP IV and aminopeptidase activities are compared. | 6360163 | [
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The aggregation of isolated human platelets in the presence of lipoproteins and prostacyclin. | Addition of prostacyclin (PGI2) temporarily inhibits platelet aggregation and permits the isolation of platelets free from plasma proteins; which have the same sensitivity as those in plasma [Moncada; Radomski & Vargas (1982) Br. J. Pharmacol. 75; 165P]. By using a modification of this technique we have established that platelets isolated from normal subjects aggregate more readily in response to ADP and adrenaline when physiological concentrations of low-density lipoproteins (LDL) are present. At high LDL | 6360159 | [
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The aggregation of isolated human platelets in the presence of lipoproteins and prostacyclin. | concentrations spontaneous aggregation occurs. High-density lipoproteins (HDL) and very-low-density lipoproteins (VLDL) had no effect on agonist-induced platelet aggregation at normal concentrations; but HDL sensitized at higher concentrations. These effects by lipoproteins are not accompanied by changes in platelet lipid content. Cyclohexanedione treatment of LDL to modify apolipoproteins appeared to abolish the sensitization effect; indicating that binding to receptors was essential for the effects of LDL | 6360159 | [
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The aggregation of isolated human platelets in the presence of lipoproteins and prostacyclin. | . LDL; but not HDL; overcame the inhibitory effect of PGI2 on platelet aggregation; except at very high concentrations of PGI2. PGI2 raised the cyclic AMP content of isolated platelets; but LDL only partially prevented this rise. These results suggest that LDL may have a greater role in platelet aggregation than previously recognized and may also regulate effects of PGI2. These findings may be of relevance to an understanding of cardiovascular diseases. | 6360159 | [
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Proteolytic activities in plasma membrane preparations from rat liver. 2. Partial purification and characterization of membrane bound endopeptidases, dipeptidyl-aminopeptidase IV and aminopeptidase. | Plasma membranes (PM) were prepared from nuclear and microsomal fractions of rat liver as described in the preceding paper. Four different proteolytic activities were studied and found to be solubilized from PM fractions after detergent treatment: endopeptidase activity at neutral and acid pH; dipeptidyl-aminopeptidase IV; and (alanine-)aminopeptidase. Both PM preparations contain a serine-endopeptidase with optimal activity against azocasein at pH 7.6. After solubilization of PM derived from microsomal fra | 6360164 | [
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Proteolytic activities in plasma membrane preparations from rat liver. 2. Partial purification and characterization of membrane bound endopeptidases, dipeptidyl-aminopeptidase IV and aminopeptidase. | ctions (PM-m) and gel filtration this activity shows an apparent molecular mass of 220 +/- 20 kD. This membrane proteinase is different from other known serine proteinases of liver cells because of its large molecular mass and an activating effect of 1;10-phenanthroline. PM derived from microsomal fractions contain additional endopeptidase with a pH optimum of 5.2 that could be inhibited by 4-chloromercuribenzoate; leupeptin and Z-Phe-Ala-diazomethylketone. Using detergent solubilization and gel filtration | 6360164 | [
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Proteolytic activities in plasma membrane preparations from rat liver. 2. Partial purification and characterization of membrane bound endopeptidases, dipeptidyl-aminopeptidase IV and aminopeptidase. | this acid endopeptidase activity in PM-m can be resolved into three peaks with apparent molecular masses (detergent forms) of 180 +/- 10; 80 +/- 10 and 35 +/- 10 kD; the latter may be cathepsin L. In addition to the endopeptidases; PM-m were shown to contain also aminopeptidases degrading Ala-Pro-pNA and Ala-pNA. The former activity; dipeptidyl-aminopeptidase IV (DPP IV); has features similar to DPP IV from other microvillus membranes (inhibition by DIFP and PMSF; pH optimum at 7.7). The Ala-pNA degrading a | 6360164 | [
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Proteolytic activities in plasma membrane preparations from rat liver. 2. Partial purification and characterization of membrane bound endopeptidases, dipeptidyl-aminopeptidase IV and aminopeptidase. | minopeptidase is inhibited by chelating agents and some bivalent heavy metal ions; but is activated by Co++-ions. Both enzymes apparently were eluted as monomers (molecular mass 180-190 kD) after gel filtration in detergent containing buffers. | 6360164 | [
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[Comparative estimation of monocompartmental methods of analyzing 99m-Tc-diethylenetriamine pentaacetic acid clearance by declining plasma levels with special reference to long term studies]. | Plasma slope clearance estimation with 99m-Tc-DTPA corresponds to total clearance determination. When activity is measured directly in cumulative serum samples; determination of the plasma slope clearance is suitable for practical applications only if simplified analytic procedures are used. Monocompartmental analytic procedure that take the late slope of the disappearance curve into account are particularly useful for GFR estimation. The A3k3-slope-clearance analysis method is a monocompartmental procedure | 6360165 | [
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[Comparative estimation of monocompartmental methods of analyzing 99m-Tc-diethylenetriamine pentaacetic acid clearance by declining plasma levels with special reference to long term studies]. | for determining slope clearance that is based on activity measurements between the 100th (120th) and 240th minute after injection. Compared with other monocompartmental procedures; the A3k3-clearance method shows a highly significant hyperbolic correlation with serum creatinine; a highly significant linear correlation with inulin total clearance; is age dependent and yields reproducible results. The sensitivity (frequency of correct function results in the case of disturbed glomerular function) of the A3k3 | 6360165 | [
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[Comparative estimation of monocompartmental methods of analyzing 99m-Tc-diethylenetriamine pentaacetic acid clearance by declining plasma levels with special reference to long term studies]. | -slope-clearance method with 99m-Tc-DTPA is particularly good. The practically important predictability of pathological A3k3-clearance results (frequency of correct results when the kidney function is truly reduced) is on the average 85%. The mean efficiency of the method is 88% (65-97% at the 95% confidence-level). | 6360165 | [
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[Demonstration of circulating immune complexes using a C1q-solid phase enzyme immunoassay]. | An enzyme-immunoassay for the quantitative determination of circulating immune complexes in human serum is described. The technique uses alkaline phosphatase-conjugated anti-human IgG to detect immune complexes bound to solid phase C1q. This assay is sensitive detecting 4 ng/ml to 60 micrograms/ml aggregated IgG. First data from normal individuals and patients with systemic lupus erythematosus show that this method is specific and sensitive for detection of circulating immune complexes. | 6360166 | [
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Peptide hydrolase activities in seedlings and hormone-treated cotyledons of pumpkin (Cucurbita pepo). | Enzymes hydrolyzing Gly-Ala-; Met-Met- and Pro-4-phenylazo-phenylamides; and N-benzoyl-L-arginine-4-nitroanilide have been identified in germinating seeds and cotyledons of pumpkin (Cucurbita pepo). The enzyme activities per cotyledon increase markedly during the germination process; but the proportion of enhancement depends on the type of enzyme species. The increase in enzyme activities is due to de novo synthesis as shown by cycloheximide treatment and is influenced by phytohormones (cytokinins and absci | 6360167 | [
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Peptide hydrolase activities in seedlings and hormone-treated cotyledons of pumpkin (Cucurbita pepo). | ssic acid). In isolated cotyledons exogenous cytokinin (benzyladenine) obviously can replace the effect of the embryo as the source of endogenous hormone. Abscissic acid counteracts the cytokinin effect. It is suggested that aminopeptidases have a biological function in reserve protein degradation of the cotyledons during seed germination. Our results do not support the assumption that the embryonic axis of the growing seedling serves as a "sink" of proteolytic products resulting in an activation of peptide | 6360167 | [
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Peptide hydrolase activities in seedlings and hormone-treated cotyledons of pumpkin (Cucurbita pepo). | hydrolases in the cotyledons; but rather de novo synthesis of these enzymes seems to be controlled by substances (phytohormones) originating from the embryo. | 6360167 | [
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