fix

cas9on.py

@@ -224,12 +224,14 @@ def create_bigwig(df, bigwig_path):
     bw = pyBigWig.open(bigwig_path, "w")
     bw.addHeader(header)
 
-    # Create a list of tuples with (chromosome, start, end, value)
-    entries = [(row['Chr'], row['Start Pos'], row['End Pos'], row['Prediction'])
-               for _, row in df.iterrows()]
+    # Create separate lists for chromosomes, starts, ends, and values
+    chromosomes = df['Chr'].tolist()
+    starts = df['Start Pos'].tolist()
+    ends = df['End Pos'].tolist()
+    values = df['Prediction'].astype(float).tolist()
 
     # Add entries to the BigWig file
-    bw.addEntries(entries)
+    bw.addEntries(chromosomes, starts, ends=ends, values=values)
 
     # Close the BigWig file
     bw.close()