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sequence | title embedding
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Suction sclerotherapy for the treatment of esophageal varices. | nan | 8979066 | [
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Staging of non-Hodgkin's gastric lymphoma with endosonography-guided fine-needle aspiration biopsy and flow cytometry. | nan | 8979069 | [
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Gastrointestinal Endoscopy: roar lion, roar. | nan | 8979075 | [
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Is your sphincterotomy really safe--and necessary? | nan | 8979074 | [
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Eradication of Helicobacter pylori as the first step in the treatment of peptic stenosis. | nan | 8979076 | [
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Esophageal dilation in the sitting, sedated patient is safe and effective. | nan | 8979080 | [
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Use of cricoid pressure to facilitate gastric insufflation and stop hiccoughs during gastroscopy. | nan | 8979082 | [
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Identification of a SPH element in the distal region of a human U6 small nuclear RNA gene promoter and characterization of the SPH binding factor in HeLa cell extracts. | Vertebrate small nuclear RNA (snRNA) gene promoters contain a distal; enhancer-like region that is composed of an octamer motif adjacent to at least one other element. Here we show that a human U6 snRNA distal region contains a SPH motif previously found in several chicken snRNA gene enhancers and the 5'-flanking region of vertebrate selenocysteine tRNA genes. SPH binding factor (SBF) was detected in either chicken or HeLa cell extracts that could bind SPH elements in a species-independent manner. Both huma | 8979085 | [
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Identification of a SPH element in the distal region of a human U6 small nuclear RNA gene promoter and characterization of the SPH binding factor in HeLa cell extracts. | n and chicken SBF required divalent cation to bind effectively to DNA. DNase I footprinting experiments indicated that human SBF specifically protected the human U6 SPH element. Furthermore; a SBF polypeptide of approximately 85 kDa was detected in both HeLa and chicken extracts following ultraviolet light-mediated cross-linking to human U6 or chicken U4 SPH elements. A part of the human U6 SPH element was quite sensitive to mutation; as demonstrated by both specific protein binding and transcription assays | 8979085 | [
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Identification of a SPH element in the distal region of a human U6 small nuclear RNA gene promoter and characterization of the SPH binding factor in HeLa cell extracts. | . From these data it is apparent that the distal regions of some RNA polymerase III- and RNA polymerase II-transcribed small RNA promoters are virtually identical in composition; and their mechanisms of transcriptional activation are possibly quite similar. | 8979085 | [
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Analysis of the negative transcriptional regulatory element in the angiotensin-converting enzyme gene. | We have characterized the sequence requirements and the protein binding properties of the previously identified transcriptional negative element present in the rabbit angiotensin-converting enzyme (ACE) gene. DNase footprinting experiments revealed that within the negative element (-715 to -610) several regions interact with proteins present in the nuclear extracts of ACE-expressing and -nonexpressing cell lines. Transfection analysis using the heterologous beta-actin promoter and mutated negative elements | 8979086 | [
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Analysis of the negative transcriptional regulatory element in the angiotensin-converting enzyme gene. | demonstrated that the SP1 site; the collagen-silencer-like sequence; and the inverted repeat elements are dispensable for their functioning. Deletion of the region between -692 to -668; however; completely eliminated the activity of the negative element; and mutation of the synapsin-silencer-like sequence present within this region vastly reduced it. This region (-692 to -668) by itself; when present in two copies; could effectively repress the activity of the beta-actin promoter. The same point mutations i | 8979086 | [
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Analysis of the negative transcriptional regulatory element in the angiotensin-converting enzyme gene. | n the silencer element that destroyed its action on the beta-actin promoter greatly increased the transcriptional efficiency of the native ACE promoter. Electrophoretic mobility shift assay using the -692 to -668 ACE silencer sequence demonstrated the formation of a DNA/protein complex. UV cross-linking of the components of this complex revealed the presence of one prominent protein of approximately 21.5 kDa. This protein may be responsible for mediating the transcriptional-repressing activity of the ACE ne | 8979086 | [
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Analysis of the negative transcriptional regulatory element in the angiotensin-converting enzyme gene. | gative element. Homology between the ACE silencer and neuronal silencer consensus sequence; together with the promoter- and tissue-independent function of the the ACE silencer; suggests this element may bind a member of a large family of common negative regulatory transcription factors. | 8979086 | [
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The human aldehyde dehydrogenase 3 gene (ALDH3): identification of a new exon and diverse mRNA isoforms, and functional analysis of the promoter. | Expression of the human ALDH3 gene is regulated in a tissue-dependent constitutive as well as drug-inducible manner. We identified a new 5'-noncoding exon (exon 1) existing at about 3 kilobase pairs (kb) upstream from the first coding exon (exon 2) of the human ALDH3 gene. Analysis of ALDH3 mRNA revealed the existence of several isoforms with different 5' regions resulting from i) usage of multiple transcriptional initiation sites of the new exon 1; ii) usage of alternative splice acceptor sites at the 3' e | 8979087 | [
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The human aldehyde dehydrogenase 3 gene (ALDH3): identification of a new exon and diverse mRNA isoforms, and functional analysis of the promoter. | nd of the new intron 1; and iii) alternative splicing out of exon 2. Usage of alternative splice acceptor sites was only found in tissues expressing ALDH3 constitutively; but not in Hep G2 induced by 2;3;7;8-tetrachlorodibenzo-p-dioxin (TCDD). Nucleotide sequence analysis and chloramphenicol acetyltransferase (CAT) expression studies showed that a strong promoter region exists at nucleotide (nt) positions -216 to +54 of the gene. Repression activities were found upstream of the -216/+54 region. Several puta | 8979087 | [
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The human aldehyde dehydrogenase 3 gene (ALDH3): identification of a new exon and diverse mRNA isoforms, and functional analysis of the promoter. | tive drug-inducible elements exist in the regulatory region. A possible regulatory mechanism for tissue-specific constitutive and inducible expression of the human ALDH3 gene is discussed. | 8979087 | [
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Vaginal birth following unmonitored labor in patients with prior cesarean section. | Following a previous cesarean section; trial labour followed by spontaneous birth is currently popular but is still debatable. In an effort to assess the risks of unmonitored labor; the outcomes of 165 patients with previous cesarean section who delayed coming to hospital were reviewed. Seventy-one patients were allowed to continue labor and 62 achieved successful vaginal delivery; a success rate of 87.3%. Sixty-one of 71 patients had an unknown uterine scar type prior to birth and; of these; 57 were delive | 8979091 | [
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Vaginal birth following unmonitored labor in patients with prior cesarean section. | red vaginally. The scar separation rate of this group was found to be 3.5% and the overall scar separation rate in our patients was 3.6%. Other than scar separation and febrile morbidity; no maternal morbidity or mortality was observed. The vast majority (98.4%) of infants delivered vaginally had 5-min Apgar scores of 7 or greater. We suggest that increasing the use of trial labor in patients with prior cesarean section; even in the presence of an unknown scar; may reduce the number of patients laboring in | 8979091 | [
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Plasma endothelin-1 levels during asphyxia in the fetal goat. | The changes in fetal carotid arterial plasma levels of endothelin-1; catecholamines; PO2; PCO2 and pH were measured after intermittent repetitive umbilical cord occlusion in late gestation pregnant goats (n = 9). Endothelin-1 levels increased to 3.58 +/- 0.28 pg/ml immediately after the onset of fetal hypoxia; a level significantly higher than the respective values in the control period (p < 0.05). The elevation of fetal plasma endothelin-1 levels correlated inversely with carotid arterial PO2 (r = -0.41; p | 8979090 | [
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Plasma endothelin-1 levels during asphyxia in the fetal goat. | < 0.05) and pH (r = - 0.43; p < 0.05). The results suggest that increased endothelin-1 levels may contribute to the maintenance of the fetal circulation during fetal asphyxia. | 8979090 | [
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A base substitution in the amino acid acceptor stem of tRNA(Lys) causes both misacylation and altered decoding. | In 1984; our laboratory reported the characterization of the first misacylated tRNA missense suppressor; a mutant Escherichia coli lysine tRNA with a C70 to U base change in the amino acid acceptor stem. We suggested then that the suppressor tRNA; though still acylated to a large extent with lysine; is partially misacylated with alanine. The results reported in this article demonstrate that is the case both in vitro and in vivo. For the in vitro studies; the mutant tRNA species was isolated from the appropr | 8979088 | [
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A base substitution in the amino acid acceptor stem of tRNA(Lys) causes both misacylation and altered decoding. | iate RPC-5 column fractions and shown to be acylatable with both lysine and alanine. For the in vivo demonstration; use was made of a temperature-sensitive alaS mutation; which results in decreasing acylation with Ala as the temperature is increased; resulting ultimately in lethality at 42 degrees C. The alaSts mutation was also used to demonstrate that the ability of the same missense suppressor; lysT(U70); to suppress a trpA frameshift mutation is not affected by the Ala-acylation deficiency. We conclude | 8979088 | [
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A base substitution in the amino acid acceptor stem of tRNA(Lys) causes both misacylation and altered decoding. | that the misacylation and altered decoding are two independent effects of the C70 to U mutation in tRNA(Lys). The influence of an alteration in the acceptor stem; which is in contact with the large (50S) ribosomal subunit; on decoding; which involves contact between the anticodon region of tRNA and the small (30S) ribosomal subunit; may occur intramolecularly; through the tRNA molecule. Alternatively; the U70 effect may be accomplished intermolecularly; for example; it may alter the interaction of tRNA with | 8979088 | [
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A base substitution in the amino acid acceptor stem of tRNA(Lys) causes both misacylation and altered decoding. | ribosomal RNA in the 50S subunit; which may then influence further interactions between the two subunits and between the 30S subunit and the anticodon region of the tRNA. Preliminary evidence suggesting some form of the latter explanation is presented. The influence of a single nucleotide on both tRNA identity and decoding may be related to the coevolution of tRNAs; aminoacyl-tRNA synthetases; and ribosomes. | 8979088 | [
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Thyroid function in early normal pregnancy: transient suppression of thyroid-stimulating hormone and stimulation of triiodothyronine. | In order to determine the effect of gestation on thyroid function in healthy subjects; we have prospectively evaluated thyroid function in pregnant individuals undergoing termination of pregnancy; and repeated the tests 2-3 months later. Venous blood was tested for human chorionic gonadotropin (hCG); thyroid-stimulating hormone (TSH); free thyroxine (FT4) and total triiodothyronine (TT3). Early pregnancy thyroid function tests showed a significant decrease (p < 0.001) in TSH and a significant increase (p < | 8979092 | [
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Thyroid function in early normal pregnancy: transient suppression of thyroid-stimulating hormone and stimulation of triiodothyronine. | 0.001) in TT3 as compared to the nonpregnant state; FT4; however; did not change significantly. In 8 (11.2%) pregnant subjects; TT3 levels were above the normal range for nonpregnant controls. Elevated thyroid function in early pregnancy is transient; and does not usually warrant antithyroid treatment. Thus; any conclusion regarding thyroid function in early pregnancy should be based on pregnant controls rather than general population controls. | 8979092 | [
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Effect of antihypertensive therapy in preeclampsia on levels of serum interleukin-4. | We investigated the effects of antihypertensive therapy on serum levels of interleukin-4 using InterTest-4 ELISA in 25 preeclamptic women; 14 were placed on treatment; while 11 were not. Comparison was made with 14 non-preeclamptic pregnant controls. Interleukin-4 levels were significantly lower in the controls than in the treated preeclamptic women (p < 0.001): 68.6 +/- 10.9 versus 106.1 +/- 13.2; 64.7 +/- 9.6 versus 119.8 +/- 11.4; 90.2 +/- 9.1 versus 124.3 +/- 15.7; and 65.6 +/- 3.6 versus 109.7 +/- 11.4 | 8979093 | [
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Effect of antihypertensive therapy in preeclampsia on levels of serum interleukin-4. | pg/ml; during antepartum; early intrapartum; late intrapartum and postpartum periods; respectively. No significant differences were detected between the therapy and no therapy subgroups (p > 0.05). This shows that antihypertensive therapy has no effect on interleukin-4 levels. | 8979093 | [
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Vertebral bone mineral density in postpartum women. | The purpose of this study was to measure the bone mineral density of the lumbar spine (L2-L4) in healthy postpartum women following term delivery; and to evaluate factors associated with bone mineral density. Using dual energy X-ray absorptiometry; the bone mineral density of the lumbar spine was determined during 1 year in 123 healthy postpartum Japanese women following term delivery. The mean bone mineral density of the lumbar spine was 1.007 +/- 0.108 g/cm2 (range 0.731-1.252 g/cm2). Thus; there were wid | 8979094 | [
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Vertebral bone mineral density in postpartum women. | e inter-individual differences. Also; this result was in agreement with the peak bone mass previously reported for other Japanese women. Multiple regression analysis and linear regression analysis showed that weight and body mass index were each significantly correlated with bone mineral density of the lumbar spine. Age; parity; height; and baby's birth weight were not contributory. | 8979094 | [
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TATA box and Sp1 sites mediate the activation of c-myc promoter P1 by immunoglobulin kappa enhancers. | In Burkitt's lymphoma (BL) cells the proto-oncogene c-myc is transcriptionally activated by chromosomal translocation to the immunoglobulin (Ig) gene loci. This activation is characterized by preferential transcription from the c-myc promoter P1 and accomplished by juxtaposed Ig enhancer elements. To identify promoter elements required for enhancer-activated P1 transcription; we studied the activation of c-myc reporter gene constructs by the Ig kappa intron and 3' enhancers. Deletion analysis defined the co | 8979089 | [
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TATA box and Sp1 sites mediate the activation of c-myc promoter P1 by immunoglobulin kappa enhancers. | re promoter with a TATA box and two adjacent GC/GT boxes upstream sufficient for basal and enhancer-activated transcription. Gel retardation assays revealed Sp1's binding affinity to the GC/GT box proximal to the TATA box to be higher than to the distal one. This difference correlated well with the resulting levels of transcription mediated by Sp1 in contransfection experiments in BL and Sp1-deficient SL2 cells. Sp3 also bound to the core promoter in vitro; but failed to transactivate in vivo. Mutation of t | 8979089 | [
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TATA box and Sp1 sites mediate the activation of c-myc promoter P1 by immunoglobulin kappa enhancers. | he distal Sp1 site moderately affected basal transcription concomitant with a modest decrease in enhancer stimulation. Mutation of the proximal Sp1 site almost entirely abolished basal as well as enhanced transcription. A considerable level of basal transcription was maintained upon mutation of the TATA box; whereas enhancer-activated transcription largely was abolished. Stable transfection of the BL cell line Raji with constructs containing core promoter mutations confirmed that the proximal Sp1 site and t | 8979089 | [
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TATA box and Sp1 sites mediate the activation of c-myc promoter P1 by immunoglobulin kappa enhancers. | he TATA box are essential for the activation of promoter P1 by the Ig kappa enhancers. | 8979089 | [
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Raised circulating levels of interleukin-6 in women with an intrauterine contraceptive device. | The aim of this study was to determine whether the presence of an intrauterine contraceptive device (IUCD) causes a systemic elevation of interleukin-6 (IL-6). The control group comprised 29 women with normal cycles and who were not using any form of contraception and the study group comprised 74 women who had had an IUCD in place for 10-24 months. Of these; 45 had intermenstrual bleeding. Systemic IL-6 was undetectable (minimum detection limit of the assay is 5 pg/ml) in the control but was raised in 20% o | 8979095 | [
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Raised circulating levels of interleukin-6 in women with an intrauterine contraceptive device. | f the women fitted with an IUCD (p < 0.01). There was no difference between IL-6 levels in IUCD users who had intermenstrual bleeding and those who did not. These results suggest that the effects of chronic IUCD usage may not be limited to the endometrium.A case-control study indicated that IUD use causes a systemic elevation of interleukin-6 (IL-6) comparable to that observed in acute and chronic inflammatory conditions. The study group was comprised of 74 women who had had a Copper T-380A device in place | 8979095 | [
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Raised circulating levels of interleukin-6 in women with an intrauterine contraceptive device. | for 10-24 months; 45 of these women reported IUD-related intermenstrual bleeding. 29 women with normal cycles who were not using any form of contraception served as controls. IL-6 levels were assessed through immunoradiometric assay. All 29 controls had undetectable circulating IL-6 levels; while 15 (20%) of cases had raised systemic levels of this cytokine. There were no differences in circulating IL-6 levels between cases who did and did not experience intermenstrual bleeding; nor was there a correla | 8979095 | [
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Raised circulating levels of interleukin-6 in women with an intrauterine contraceptive device. | tion between IL-6 levels and the day of the cycle on which a blood sample was randomly collected. It is unclear whether the circulating IL-6 observed in this study is an "overspill" from locally produced IL-6 or indicative of a more generalized systemic immunoactivation. Recommended are studies that monitor local and systemic production of IL-6 in IUD users in conjunction with other parameters of an inflammatory response; including C-reactive protein. | 8979095 | [
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Selection of criteria for the treatment of ovarian cysts on the bases of ultrasound and cytology. | The major objective of the present study was to determine whether leakage of ovarian cyst cells occurs when the cysts are punctured inside a container with saline. Other subjectives were to determine the sensitivity and specificity of cytology in the differentiation between malignant and benign cysts by comparing cytology data with histology data; and also to determine whether ultrasound can provide consistent evidence for predicting whether the cyst is benign or malignant; thus permitting the selection of | 8979096 | [
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Selection of criteria for the treatment of ovarian cysts on the bases of ultrasound and cytology. | patients with benign cysts for puncture rather than surgical exeresis. A cytologic study was carried out on the contents of 62 ovarian cysts diagnosed by ultrasound and clinical examination; and excised from 51 patients submitted to exploratory laparotomy. The cyst was excised and immersed in a container with saline outside the surgical field. The cyst was punctured and its contents aspirated through the wall of the container. Five samples were obtained for cytology: (1) presurgical abdominal wash; (2) sali | 8979096 | [
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Selection of criteria for the treatment of ovarian cysts on the bases of ultrasound and cytology. | ne before cyst puncture; (3) fluid from the punctured cyst; (4) saline after cyst puncture; and (5) postoperative abdominal wash. The materials obtained were submitted to centrifugation and slides of the precipitate were prepared for cytology. A total of 248 slides were studied. Slides of cysts with good cellularity were stained with silver to study the nucleolar organizer regions. Analysis using Kappa statistics showed excellent agreement between the cytologic and histologic diagnoses (p < 0.001). The sens | 8979096 | [
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Selection of criteria for the treatment of ovarian cysts on the bases of ultrasound and cytology. | itivity of the cytologic study of the cyst was 75% and the specificity 100%; with a positive predictive value of 100%; a negative predictive value of 92%; and 93% accuracy. The present results led us to conclude that cell leakage into physiological saline occurred after puncture and that cytologic study of cysts valuable in the diagnosis of benign and malignant processes. | 8979096 | [
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Laparoscopically assisted vaginal hysterectomy as an alternative to abdominal hysterectomy. A pilot study. | Laparoscopically assisted vaginal hysterectomy (LAVH) allows for conversion of some abdominal hysterectomies to a vaginal route. LAVH may be particularly useful when adnexectomy is indicated. In this study the authors evaluate the results obtained in a series of 21 patients prospectively selected for LAVH. The mean operative time was 96 min (range 58-155); with an estimated blood loss of 218 ml (range 50-510). The mean uterine weight was 320 g (range 105-610). Intraoperative complications were limited to on | 8979097 | [
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Laparoscopically assisted vaginal hysterectomy as an alternative to abdominal hysterectomy. A pilot study. | e case of bladder injury which occurred during the vaginal phase of the procedure. No patient required heterologous blood transfusion. No postoperative complications were observed. The average length of hospitalisation was 4.4 days (range 2-9). The authors conclude that LAVH allows for conversion of a significant number of abdominal hysterectomies to a vaginal route and that with respect to the former it is definitely less costly since it allows for earlier hospital discharge and resumal of a normal lifesty | 8979097 | [
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Electro-oviductogram: a study of the electromechanical activity of the canine oviduct. | The purpose of this communication was to study the electromechanical activity of the oviduct aiming at the characterization of a normal 'electro-oviductogram' (EOG). The oviduct of 14 bitches was exposed and 3 monopolar silver-silver chloride electrodes were sutured to the duct serosa. The oviduct pressure was also measured. Direction of propagation of the electric activity was assessed by duct myotomies. Slow waves or pacesetter potentials (PPs) were recorded from the 3 electrodes of each dog. They were id | 8979098 | [
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Electro-oviductogram: a study of the electromechanical activity of the canine oviduct. | entical from the 3 electrodes; had a regular rhythm and were reproducible. The PPs were followed by bursts of action potentials (APs) which were associated with increased oviduct pressure. PPs and APs were recorded from electrodes proximal but not distal to the oviduct myotomy; indicating that they were propagated from the fimbrial end of the oviduct towards the uterus. A 'pacemaker' initiating these waves might exist at this fimbrial end. The increased oviduct pressure associated with APs might point to a | 8979098 | [
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Electro-oviductogram: a study of the electromechanical activity of the canine oviduct. | contractile activity that may be responsible for initiating the oviduct peristaltic activity. In conclusion; the current study could characterize an EOG for the normal canine oviduct. It is suggested that; after further studies; the EOG may be included as an investigative tool in the diagnosis of oviduct disorders. | 8979098 | [
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The association of age and sexual history with the severity of the histological findings in women with cervical intraepithelial neoplasia. | We examined the demographic and behavioral data of 203 patients with histological diagnosis of cervical intraepithelial neoplasia (CIN) to assess putative predictors of the most severe alterations (CIN grade III). The variables significantly associated with the histological diagnosis of CIN III were time since first intercourse (odds ratio 5.33; 95% CI 1.80-16.15); time since menarche (odds ratio 4.62; 95% CI 1.39-16.05) and age (odds ratio 2.48; 95% CI 1.32-4.67). After multivariate analysis; the only inde | 8979099 | [
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The association of age and sexual history with the severity of the histological findings in women with cervical intraepithelial neoplasia. | pendent variable still associated with CIN III was the time since first intercourse (odds ratio 6.11; p < 0.05). It is concluded that; in patients with recently diagnosed CIN; the time since first intercourse-rather than age; age at first intercourse or lifetime number of sex partners-is the information best associated with the severity of the disease. | 8979099 | [
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MIB 1 immunostaining in cervical intraepithelial neoplasia: prognostic significance in mild and moderate lesions. | MIB 1 is a new monoclonal antibody which recognizes nuclei of proliferating cells throughout the cell cycle except during the G0 and early G1 phases. In the present study we analyzed the MIB 1 immunostaining as an index of cellular proliferation in cervical intraepithelial neoplasia (CIN) and microinvasive carcinoma; with the aim to identify a relationship with the degree of dysplastic lesion and the risk of neoplastic progression. A correlation between the MIB 1 index and human papillomavirus (HPV) DNA pre | 8979100 | [
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MIB 1 immunostaining in cervical intraepithelial neoplasia: prognostic significance in mild and moderate lesions. | sence was also investigatedCervical bioptic samples were consecutively obtained from 86 women who attended our Colposcopic Service from January 1993 to June 1994; because of abnormal pap smears suspicious for cervical dysplasia and/or HPV infection. On histologic evaluation; 41 women had CIN; 23 cervical condyloma; and 22 squamous metaplasia. Ten patients with microinvasive squamous cervical carcinoma; matched for age and demographic characteristics; were selected from our series of cervical carcinomas and | 8979100 | [
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MIB 1 immunostaining in cervical intraepithelial neoplasia: prognostic significance in mild and moderate lesions. | immunohistochemically analyzed. The expression of primary tumor cellular proliferation was immunohistochemically evaluated by monoclonal MIB 1 antibody (Immunotech; Marseille Cedex; France) on microwave oven-processed formalin-fixed paraffin-embedded tissue. Positive staining was expressed as the percentage of positive cells per 10(3) counted dysplastic cells for each case.A progressive significant increase in positive MIB 1 immunostaining was observed from squamous metaplasia to microinvasive carcinoma thr | 8979100 | [
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MIB 1 immunostaining in cervical intraepithelial neoplasia: prognostic significance in mild and moderate lesions. | oughout the CIN lesions (p < 0.001). Considering only CINs; the MIB 1 index showed a significant increase with respect to CIN degrees (p < 0.001); no correlation was found between MIB 1 immunostaining and HPV infection; and lesion size. By analyzing the MIB 1 index with respect to CIN outcome in mild and moderate dysplasias; regressive lesions had lower values of MIB 1 immunostaining; while persistent and progressive lesions presented significantly higher positivity (p < 0.001).Our data demonstrated: (1) th | 8979100 | [
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] |
MIB 1 immunostaining in cervical intraepithelial neoplasia: prognostic significance in mild and moderate lesions. | at positive MIB 1 immunostaining increased progressively from squamous metaplasia to CIN and microinvasive carcinoma; suggesting that neoplastic transformation is associated with a dysfunctional proliferation of cervical epithelium; (2) that there was a significant correlation between the MIB 1 index and CIN degree but not with respect to HPV DNA presence; and (3) that MIB 1 immunostaining might be useful for a clinical evaluation of mild and moderate dysplastic lesions. However; a much larger study needs t | 8979100 | [
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MIB 1 immunostaining in cervical intraepithelial neoplasia: prognostic significance in mild and moderate lesions. | o be done over a longer period of time to truly determine the value of the technique in prognostically predicting which lesions might or might not regress. | 8979100 | [
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Serum levels of cytokeratin 19 fragments in cervical cancer. | Serum levels of a cytokeratin 19 fragment; Cyfra 21-1; were measured using an immunoradiometric assay in 33 women before initial treatment and in 8 women with recurrent tumor. The serum level of Cyfra 21-1 was significantly increased in women with tumors of advanced stage (FIGO) and those with recurrence. The incidence of positivity for Cyfra 21-1 tended to increase with tumor spread. Compared with squamous cell carcinoma (SCC) antigen; the sensitivity of Cyfra 21-1 was comparable to that of SCC antigen. Th | 8979101 | [
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Serum levels of cytokeratin 19 fragments in cervical cancer. | e serum level of Cyfra 21-1 and that of SCC antigen showed a positive correlation. While the use of the serum Cyfra 21-1 level may be limited in cervical cancer by its relatively low sensitivity; a combination assay of Cyfra 21-1 and SCC antigen may be useful in the diagnosis and follow-up of patients with cervical squamous cell carcinoma. | 8979101 | [
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Placental isoferritin patterns during first-trimester intrauterine gestation. | In this study; serum placental ferritin (PLF) levels were measured among 33 pregnant women with normal; singleton; ongoing first-trimester gestations and compared with 22 nonbleeding; missed and 14 incomplete abortions. Diagnoses were based on clinical; sonographic; endocrinologic and histopathologic criteria. At admission and before uterine evacuation of the pregnancy products; venous blood was collected from the groups for PLF determination. The mean +/- SD serum PLF levels were 18 +/- 14; 21 +/- 37; and | 8979102 | [
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Placental isoferritin patterns during first-trimester intrauterine gestation. | 3.9 +/- 6.2 U/ml in normal gestations; missed and incomplete abortions; respectively. Significant differences in PLF levels were found among normal pregnancies and incomplete abortions (p < 0.05); whereas borderline significance was found in the missed abortion group (p = 0.53). No correlation was found between serum beta hCG and PLF for the various pregnancy groups. Low PLF levels in pathologic gestation may reflect abnormal trophoblastic activity. Whether these play a causal role or are the casual outcome | 8979102 | [
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Placental isoferritin patterns during first-trimester intrauterine gestation. | of pathologic gestation should be the subject of further evaluation. | 8979102 | [
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Spontaneous perforated pyometra presenting as pneumoperitoneum. | Spontaneous perforated pyometra presenting as pneumoperitoneum is extremely rare.An 80-year-old Japanese female with spontaneous perforating pyometra presenting as pneumoperitoneum is reported. The patient came to our institute with severe abdominal pain. Routine abdominal examination showed muscular defense; and plain chest roentgenograms revealed infradiaphragmatic free gas. Subsequent computed tomography also demonstrated pneumoperitoneum. Laparotomy was performed on the basis of a tentative diagnosis of | 8979103 | [
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Spontaneous perforated pyometra presenting as pneumoperitoneum. | perforation of the gastrointestinal tract but revealed a perforated pyometra. A simple hysterectomy was performed. The histological diagnosis of the surgical specimen was acute endometritis without neoplasm. The present report is the third case of spontaneous perforated pyometra with pneumoperitoneum to date.Although uterine disease presenting as pneumoperitoneum is rare in elderly patients with an acute abdomen; the possibility of a perforated pyometra should be considered in the differential diagnosis. | 8979103 | [
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Mechanisms, markers and management of hypercoagulable states. | Information on hypercoagulability can be achieved through the measurement of peptides that are generated when zymogens are activated; of the active enzymes; or of the complexes that form in the circulation when enzymes are inactivated by their plasmatic inhibitors. These assay methods have dramatically improved our understanding of the mechanisms which regulate coagulation in health and disease. However; their choice and use as predictors of thrombotic events and to monitor anticoagulant treatment still nee | 8979106 | [
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Urethral caruncle: an unusual presentation of ovarian tumour. | Secondary female urethral cancer presenting as urethral caruncle is extremely rare. We report a primary ovarian adenocarcinoma in a 78-year-old female patient presented initially as a urethral caruncle. This case represents the first reported case in the world literature. The clinical features; investigations and pertinent literature are reviewed. | 8979105 | [
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A case of Pendred's syndrome presenting with amenorrhea. | Pendred's syndrome is manifested by congenital sensorineural deafness in association with familial goiter due to defective organic binding of iodine in the thyroid gland. The majority of patients with Pendred's syndrome are euthyroid. We report on an unusual case of a patient with Pendred's syndrome presenting with amenorrhea and late-onset hypothyroidism. | 8979104 | [
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] |
Molecular basis for protein C hereditary deficiency. | The clinical presentation of hereditary protein C deficiency is highly variable. Homozygosity and compound heterozygosity have been linked to severe thrombotic complications early in the life. Heterozygous patients have a moderate form of the disease with deep venous thrombosis during adulthood. In the French population; we found 53 different mutations in 90 families. The amount of the protein C produced by the mutant allele as well as the genetic status partly account for the variable clinical expression. | 8979107 | [
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] |
Molecular basis for protein C hereditary deficiency. | Other gene may also be involved: Arg 506 to Gln factor V mutation shows a frequency of 10 to 20% in symptomatic protein C deficient patients. Some protein C gene mutations are associated with a non functional circulating protein; most of them are located in the GLA domain and in the serine protease domain. The biochemical characterization of a few of theses variants has shown the important role of some amino acids on the activation and the mechanism of action of protein C. | 8979107 | [
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Consequences of transcellular biosynthesis of leukotriene C4 on organ function. | Formation of eicosanoids is a special mode of cell communication whereby production of eicosanoids by mixed cell populations differs from that expected from each individual cell. Transcellular biosynthesis of leukotriene C4 occurs via transfer of the reactive intermediate leukotriene A4 from neutrophils to vicinal acceptor cells devoid of 5-lipoxygenase activity such as platelets or vascular cells. Evidence for the in vivo relevance of transcellular eicosanoid metabolism results from experiments using the i | 8979109 | [
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Consequences of transcellular biosynthesis of leukotriene C4 on organ function. | solated beating rabbit heart perfused with activated neutrophils. The resultant leukotriene C4 synthesis is timely related to the pressor response of the coronary arteries and inflammatory damage of the heart by edema formation and neutrophil infiltration into the organ. Blockade of leukotriene C4 synthesis by 5-lipoxygenase inhibitors or leukotriene C4 actions by respective receptor antagonists facilitated significant protective effects. Further confirmation of the potential role of LTC4 in myocardial isch | 8979109 | [
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Consequences of transcellular biosynthesis of leukotriene C4 on organ function. | emia comes from in vivo studies in the rabbit. | 8979109 | [
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Injury models of the vascular endothelium: apoptosis and loss of thromboresistance induced by a viral protein. | Endothelial injury caused by viruses usually involves viral replication or transformation. We report a novel mechanism of endothelial damage by a toxic viral protein. We have isolated a new retrovirus from hemangiosarcomas which appeared among layer hens. The isolated avian hemangiosarcoma virus (AHV) is capable of inducing hemangiomas in hens in-vivo and causes a cytopathic effect (CPE) and loss of thromboresistance in cultured bovine aortic endothelial cells (BAEC). These effects do not require viral repl | 8979110 | [
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Injury models of the vascular endothelium: apoptosis and loss of thromboresistance induced by a viral protein. | ication and can be induced by purified AHV envelop glycoprotein (gp85). AHV causes CPE in BAEC through a typical programmed cell death (apoptosis). Quiescent G0/G1-BAEC are much more sensitive to AHV induced apoptosis than actively dividing cells. These experiments demonstrate the capacity of viral proteins to affect the integrity and functionality of vascular endothelial cells. | 8979110 | [
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Platelet-polymorphonuclear leukocyte functional interactions: role of adhesive molecules. | nan | 8979108 | [
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Dietary fatty acids in athero-thrombogenesis: influence of palm oil ingestion. | Dietary experiments; performed in metabolic wards; gave rise to predictive regression equations relating changes of plasma cholesterol concentration to the intake of fatty acids of the diet. It has been established that polyunsaturated fatty acids diminish and most saturated fatty acids increase plasma cholesterol concentration. This information led to expect that dietary use of palm oil may induce an unfavorable plasma lipoprotein profile. This has not been the case as shown in various dietary experiments. | 8979111 | [
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Dietary fatty acids in athero-thrombogenesis: influence of palm oil ingestion. | The reasons for this discrepancy is discussed. The influence of palm oil enriched diets on prothrombotic variables show that platelets are not affected in their function during prolonged dietary intervention. It is important to continue research on the effects of palm oil based diet on plasma fibrinogen; factor VII. There is still discordant information in this field. | 8979111 | [
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Which experimental model to choose to study arterial thrombosis and evaluate potentially useful therapeutics? | The use of experimental models of arterial thrombosis both in vivo and ex vivo in animals and ex vivo in humans is an obligatory step for the understanding of mechanisms involved in thrombogenesis as well as in the evaluation of anti-thrombotic therapeutics. Arterial thrombogenesis is a complex phenomenon which involves multiple systems; mechanisms and parameters. Therefore studies of thrombogenesis from a pathological as well as a therapeutic point are necessary for understanding this problem in its entire | 8979112 | [
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Which experimental model to choose to study arterial thrombosis and evaluate potentially useful therapeutics? | ty. For these reasons; it is necessary to use models as representative as possible of the human pathological condition. Besides these theoretical requirements; practical needs have also to be fulfilled (accessibility of the models; adaptation to the type of the technique to different animal model and/or of the size of the animal to the amount of molecule available; cost...) which necessarily lead to some compromises. In this review we have tried to underline the criteria for the choice; characteristics; adv | 8979112 | [
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Which experimental model to choose to study arterial thrombosis and evaluate potentially useful therapeutics? | antages and disadvantages of the major models commonly accepted and used; in such a form that the reader who may not be an expert in the field would be led either to a choice of a particular model for a specific purpose or to appreciate a paper or a report based on an experimental model of arterial thrombosis. In vitro models of arterial thrombosis are so far removed from reality and due to their nature can generate so much artifacts that we have omitted their discussion from this paper. | 8979112 | [
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Modulating platelet function with selective thrombin inhibitors. | In addition to its pivotal role in blood coagulation; thrombin is one of the most important agonists for platelet recruitment and aggregation. Thrombin inhibitors impede thrombin-induced platelet aggregation but have no effect on aggregation induced by other agonists. A review is presented of selective thrombin inhibitors now in clinical investigation; some of which are also orally active. | 8979113 | [
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New thoughts on strategies for modulating platelet function through the inhibition of surface receptors. | Platelets play a key role in arterial thrombosis. Inhibition of platelet function forms an essential part of anti-thrombotic therapy. Although aspirin is a cost-effective drug which offers considerable scope in the prevention of arterial thrombosis; it only inhibits part of the platelet activation process. Much recent progress has concerned the inhibition of surface receptors of platelets. Ticlopidine and clopidogrel specifically interfere with the ADP-induced activation pathway. Inhibitors of GP IIb-IIIa c | 8979114 | [
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New thoughts on strategies for modulating platelet function through the inhibition of surface receptors. | omplexes; including c7E3; block the final step of the platelet aggregation process as used by all physiologic agonists and are proving powerful therapeutic agents. This review looks at these and future advances in the construction of therapeutic strategies aimed at combatting arterial thrombosis. | 8979114 | [
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Analysis of the kinetics of factor X activation by tissue factor-factor VIIA. | In this paper we consider the assumptions that apply to the classical Michaelis-Menten enzyme kinetics and examine their applicability to a two-dimensional catalytic system. While we specifically address the kinetics of factor X activation catalyzed by a complex of tissue factor and factor VIIa; these concepts are applicable to any system in which both enzyme and substrate are membrane-bound. We emphasize the fact that the membranes become crowded with substrate and product molecules; thus rendering the ass | 8979116 | [
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Analysis of the kinetics of factor X activation by tissue factor-factor VIIA. | umption of "sparseness" inappropriate. Data from computer simulations are considered as well as preliminary laboratory experiments. | 8979116 | [
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