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Renal replacement therapy in acute renal failure. | The choices regarding renal replacement therapy in acute renal failure have become more complex. The underlying prognosis of the patient should be considered when making the choice of a dialysis treatment. The attending nephrologist must also have a working knowledge of the potential advantages of various dialyzers and continuous renal replacement therapies to maximize the potential for recovery of patients with acute renal failure. | 8978993 | [
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Hemodialysis vascular access: emerging concepts. | Vascular access complications remain a leading cause of morbidity and hospitalization in patients on chronic maintenance hemodialysis. Although arteriovenous fistulas remain the dialysis access of choice; an increasing percentage of patients are dialyzed using synthetic grafts or permanent catheters. The most frequent vascular access complications are the development of stenosis and of thrombosis. In recent years; increasing attention is being paid to screening techniques designed to detect vascular access | 8978994 | [
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Hemodialysis vascular access: emerging concepts. | stenosis prior to thrombosis. Aggressive screening techniques coupled with interventional procedures such as angioplasty may reduce the incidence of graft thrombosis. Once thrombosis has occurred; mechanical or pharmacomechanical thrombolysis in conjunction with angioplasty may be an effective alternative to surgical thrombectomy. | 8978994 | [
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Adequacy of peritoneal dialysis. | Landmark studies relevant to the adequacy of peritoneal dialysis have recently been published. The Canada/USA peritoneal dialysis study has shown a relationship between clearance indices and patient survival rate; but the study is confounded by residual renal function. Registry data show conflicting results concerning survival rates on peritoneal dialysis and haemodialysis. The complicated pathogenesis of malnutrition in dialysis patients is now becoming further appreciated. | 8978995 | [
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Hepatitis C in dialysis and transplantation. | The identification of the hepatitis C virus as the major cause of liver disease both in dialysis patients and in transplant patients has focused attention on the epidemiology and the impact of continuing infection in these patient groups. The present understanding of transmission; the extent of liver injury; the influence on outcome measures and treatment has grown substantially in a relatively short time. | 8978996 | [
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Update on the status of pancreas-kidney transplantation. | Patient and graft survival rates after combined kidney and pancreas transplantation continue to improve. Percutaneous and cystoscopic pancreas biopsies are safe and aid in the diagnosis of acute rejection. Primary enteric drainage of pancreatic exocrine secretions is being re-evaluated to avoid the morbidity associated with bladder drainage. The use of FK506 might reduce the number of acute rejection episodes with no diabetogenic side effects. More studies have been presented which demonstrate that pancreas | 8978997 | [
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Update on the status of pancreas-kidney transplantation. | transplantation might improve diabetic microangiopathy and polyneuropathy and protect the transplanted kidney from diabetic nephropathy. | 8978997 | [
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Mycophenolate mofetil in kidney transplantation. | Mycophenolate mofetil is a new immunosuppressive agent recently released for clinical use; in conjunction with steroids and cyclosporine; for the prevention of acute rejection in renal transplant patients. It inhibits the enzyme inosine monophosphate dehydrogenase; which is essential for de-novo synthesis of guanine nucleotides. The result is lymphocyte-selective arrest of cell division; with little effect on other tissues. In clinical trials in renal transplant; its efficacy and safety have been establishe | 8978998 | [
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Mycophenolate mofetil in kidney transplantation. | d: mycophenolate mofetil reduces acute rejection episodes by approximately 50%; with the principal toxicity being gastrointestinal; and perhaps some increase in cytomegalovirus infection. Overall; mycophenolate mofetil (plus cyclosporine and steroids) has a toxicity profile similar to that of azathioprine but with considerably increased efficacy. Despite its cost; mycophenolate mofetil is likely to be used in many renal transplant centres to reduce acute rejection; especially in the first year. Future trial | 8978998 | [
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Mycophenolate mofetil in kidney transplantation. | s are necessary to establish its long-term role in renal transplantation and its usefulness in the transplantation of other organs. | 8978998 | [
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Xenotransplantation. | Xenotransplantation of pig organs into humans represents an attractive solution to the worldwide organ shortage. The survival of pig organs in primates has so far primarily been hampered by hyperacute rejection of the xenograft. The immunological mechanisms which underlie hyperacute rejection have recently been clarified. These important elucidations; together with the introduction of genetic engineering in the field of transplantation; have led to the production of genetically manipulated animals with orga | 8978999 | [
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Xenotransplantation. | ns that are now not susceptible to the immunological factors responsible for hyperacute rejection. In this article the recent advances in the understanding of hyperacute rejection are reviewed and the latest biomolecular approaches to overcome hyperacute rejection are described. | 8978999 | [
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Measuring the adequacy of peritoneal dialysis: is there a link with nutrition and outcome? | Adequacy of peritoneal dialysis is predictive of outcome but the absolute evidence for setting optimal levels or targets is still lacking. The link between adequacy and nutrition is also tenuous and appears to be based on a mathematical relationship. The ability of standard continuous ambulatory peritoneal dialysis regimes to provide adequate dialysis may be limited in anuria and in large individuals. | 8979001 | [
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Measuring the adequacy of hemodialysis access. | Vascular access morbidity is still an important problem. The risk of thrombosis increases in prosthetic grafts as the access flow decreases below 600 ml/min; whereas native arteriovenous accesses can maintain patency at much lower flows; in some cases flows below those needed to deliver adequate dialysis. Intra-access pressures are also dissipated differently in the two access types. These differences between native and prosthetic grafts determine the utility of the monitoring techniques that are useful for | 8979002 | [
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Measuring the adequacy of hemodialysis access. | detecting stenosis and assessing function in the two access types. Modern non-urea methods for measuring access recirculation are therefore useful in native but not in graft hemoaccesses. Direct or indirect intra-access pressure measurements and direct flow measurements are most useful in grafts to detect and then correct lesion before thrombosis. | 8979002 | [
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Screening for cardiovascular disease in dialysis patients. | The burden of cardiac disease in dialysis patients is high; resulting from cardiomyopathy and from coronary artery disease. Asymptomatic dialysis patients; patients with heart failure; patients with symptomatic ischaemic heart disease; and patients being evaluated for renal transplantation all require investigation to determine the presence or the extent of heart disease. Echocardiography is very useful for screening for cardiomyopathy; but should be undertaken when patients are euvolaemic. Non-invasive tes | 8979003 | [
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Screening for cardiovascular disease in dialysis patients. | ts for coronary artery disease; particularly exercise-based tests; are less sensitive and less specific in dialysis patients than in the general population. Dobutamine stress echocardiography may be more sensitive in patients with renal failure than other investigations. Patients with abnormal screening tests or symptomatic ischaemic heart disease should undergo coronary angiography and subsequent revascularization procedures if indicated. | 8979003 | [
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Radiographic evaluation of the renal vasculature. | Renal artery stenosis is an important cause of hypertension and progressive renal insufficiency. Additionally; there is increasing concern that renovascular disease is a significant; but previously-unrecognized; cause of end-stage renal disease in certain subsets of patients. Advances in revascularization techniques offer a greater opportunity for blood pressure control and for the restoration or preservation of renal function. Accurate imaging of the renal vasculature; however; is essential for the proper | 8979004 | [
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Radiographic evaluation of the renal vasculature. | selection of those individuals who might best benefit from such intervention. Although conventional or digital subtraction arteriography remains the gold standard diagnostic test; significant advances in non-invasive imaging techniques now offer the clinician several options for the accurate diagnosis of hemodynamically significant renovascular disease. These techniques include captopril renography; duplex ultrasonography; magnetic resonance angiography; and spiral/fast computed tomography. In this review; | 8979004 | [
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Radiographic evaluation of the renal vasculature. | the advantages and limitations of these imaging techniques are compared and contrasted with an emphasis on their usefulness in screening for renovascular disease. Also reviewed are recent applications of these techniques for measurement of renal function; predicting outcome of revascularization; and the clinical monitoring of patients with renovascular disease managed either medically or by revascularization. | 8979004 | [
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The role of angiotensin I-converting enzyme gene polymorphism in renal disease. | Some renal diseases (e.g. diabetic nephropathy and IgA glomerulonephritis) cluster within families; consistent with a strong genetic component for the development or progression of these diseases; or both. In this context it is attractive to examine the insertion/deletion polymorphism of the angiotensin I-converting enzyme. This polymorphism determines the concentration of angiotensin I-converting enzyme not only in serum; but also in tissues and thereby presumably the locally available concentration of ang | 8979005 | [
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The role of angiotensin I-converting enzyme gene polymorphism in renal disease. | iotensin II. Several studies have examined whether this polymorphism is associated with the development of diabetic nephropathy; but most of these failed to show such an association. Studies in patients suffering from IgA glomerulonephritis or other renal diseases; including diabetic nephropathy; demonstrated that the insertion/ deletion polymorphism plays a role in the progression of renal diseases and in the response to treatment with angiotensin I-converting enzyme inhibitor. | 8979005 | [
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Folic acid to prevent neural tube defects: scientific advances and public health issues. | Folic acid prevents some neural tube defects by correcting abnormal homocysteine metabolism. A defective gene coding for a homocysteine-related enzyme has been shown in people with neural tube defects. An extensive educational campaign will be required to alert women to the need to take 0.4 mg/day of folic acid during their childbearing years. | 8979009 | [
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Medications in pregnancy. | Chronic medical problems and the various medications available to treat them represent an enormous volume of information for the practicing obstetrician. The agents commonly encountered are reviewed here and current literature is explored; where available; including the literature on folates in pregnancy. | 8979010 | [
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Adverse consequences of prenatal illicit drug exposure. | Our appreciation of the impact on health of illicit drug use is growing. Once considered a maternal risk; prenatal drug exposure may target fetal neurobehavior; affecting attention and learning as the child grows into adulthood. Cocaine; opiates; marijuana; and amphetamines have each been scrutinized for adverse actions on placental transport; fetal behavior states; newborn withdrawal; and childhood learning and attentive skills. Neurotransmitter analysis in the animal model after prenatal drug exposure now | 8979011 | [
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Adverse consequences of prenatal illicit drug exposure. | provides biological support for these clinical findings. The increasing prevalence of drug use by pregnant women; the effect of illicit drug use on transmission of the human immunodeficiency virus; and the maternal and fetal consequences of illicit drug exposure make illicit drug use in pregnancy a central challenge in maternal-fetal medicine and a need-to-know field in general obstetrics. | 8979011 | [
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Prediction of prematurity. | Preterm birth is the leading cause of perinatal morbidity and mortality. A poor understanding of the underlying pathophysiology of spontaneous preterm labor and preterm premature rupture of membranes has limited our ability to identify those women at highest risk for spontaneous preterm birth. There is increasing evidence that inflammation of the upper genital tract may play a major role in the pathogenesis of preterm labor and preterm premature rupture of membranes. Newer markers of infection and inflammat | 8979013 | [
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Prediction of prematurity. | ion (e.g. bacterial vaginosis; fetal fibronectin; interleukin-6) may make earlier diagnosis possible and may direct potential therapeutic interventions. A better understanding and more accurate diagnosis of well known risk factors (e.g. cervical dilatation) may also improve treatment options. Additionally; combinations of older risk factors and newer; more sensitive diagnostic methods may greatly increase our ability to predict preterm birth and to identify women who might benefit most from directed interve | 8979013 | [
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Congenital malformations. | Obstetricians' epidemiological knowledge and their awareness of associated risk factors are essential for the primary prevention of birth defects. Screening and early diagnosis of congenital malformations is an expanding field in general obstetrics. Cardiac defects are the most frequent type of congenital malformation; and early detection is possible. | 8979012 | [
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Infection and prematurity: evidence-based approaches. | The real cost savings arising from effectively preventing or treating preterm birth ascribable to prevalent reproductive tract infections are demonstrated in the lives of children saved from premature death and personal; biologic and economic impairment. Three meta-analyses demonstrate neonatal and maternal benefits from adjunctive antibiotic treatment of mothers suffering preterm premature rupture of membranes. The effects of adjunctive antibiotic treatment of women with preterm labor are inconsistent; but | 8979014 | [
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Infection and prematurity: evidence-based approaches. | single-agent regimens using erythromycin and clindamycin are effective and provide coverage for group B streptococcus. Providers and payers now have evidence-based; clinically proven and cost-saving opportunities to prevent important numbers of preterm births; as well as preventing individual children suffering from being 'born too soon'. | 8979014 | [
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Pregnancy hypertension and uterine artery Doppler ultrasound. | The application of uterine artery Doppler velocimetry as a screening test for pre-eclampsia and other adverse pregnancy complications is still considered a research tool by most obstetricians. Increasing use of colour Doppler ultrasound has allowed a better understanding of pathophysiological changes in the uterine circulation. This in turn has refocused attention on the potential use of uterine artery Doppler velocimetry as part of our antenatal screening strategy. This review summarizes the most recent wo | 8979015 | [
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Pre-term pre-labour amniorrhexis. | In pregnancies complicated by pre-term pre-labour aminorrhexis; there is a risk of intra-uterine infection; which is associated with maternal and perinatal mortality and morbidity. The causes of neonatal death in pregnancies with aminorrhexis are prematurity; pulmonary hypoplasia and sepsis. In the management of pregnancies with pre-term pre-labour amniorrhexis; it is essential to distinguish between those with and without intra-uterine infection. If there is no infection at presentation; it is unlikely tha | 8979016 | [
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Pre-term pre-labour amniorrhexis. | t this will develop; and in such cases there is no benefit from hospitalization; bed rest; prophylactic tocolytics or antibiotics. The group with evidence of intra-uterine infection go into spontaneous labour within a few days of amniorrhexis; in this group; the main determinant for the appropriate management is the gestational age at amniorrhexis. | 8979016 | [
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Recurrent second trimester miscarriage. | Understanding the aetiology of a second trimester miscarriage allows the obstetrician to assess the recurrence risk in the next pregnancy and guides future management. Causes include anatomical abnormalities; cervical incompetence; genital tract infection; multiple pregnancy; auto-immune factors; vaginal bleeding; and fetal abnormalities. Transvaginal ultrasound of the cervix in pregnancy may have a role in the diagnosis of cervical incompetence. There is an association between genital tract infection and s | 8979017 | [
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Thromboembolism in pregnancy: an overview. | Thromboembolism is a major cause of maternal mortality. Recent advances in the management of thromboembolism in pregnancy include the discovery of a new; inherited thrombophilia; an improved understanding of the indications for thromboprophylaxis; and the increased use of low-molecular-weight heparin instead of unfractionated heparin. | 8979019 | [
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Human immunodeficiency virus and pregnancy. | There are now proven ways to reduce the transmission of human immunodeficiency virus from infected mothers to their offspring; including the use of zidovudine during pregnancy and labour; and avoidance of breast-feeding. Therefore; screening programmes should be extended to enable at-risk mothers to benefit from these advances. | 8979018 | [
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CD34 progenitor cell subset analyses in normal human bone marrow and marrow harvested after intermediate-dose chemotherapy. | Previous attempts to characterize harvested marrow and peripheral blood stem cell (PBSC) in order to predict time to and quality of engraftment post autologous bone marrow transplant (autoBMT) have included use of in vitro colony forming unit (CFU) assays. These assays are hampered by interlaboratory variability and are not uniformly predictive. CD34 quantification by flow cytometric technique has also been used to assess the quality of harvested marrow and PBSC. However; a lack of standardization has hampe | 8979021 | [
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CD34 progenitor cell subset analyses in normal human bone marrow and marrow harvested after intermediate-dose chemotherapy. | red direct comparison of published reports. We sought to characterize these early lineage-committed CD34+ progenitor cells from non-ficolled harvested marrow with six progenitor cell (PC) panels containing CD34 antibody plus two additional early lineage markers; using multiparameter flow cytometry. The specific gating technique including simultaneous CD34-PE vs. side scatter and forward vs. side scatter; was verified using morphologic analyses of sorted CD34+ cells. An ungated file was initially acquired to | 8979021 | [
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CD34 progenitor cell subset analyses in normal human bone marrow and marrow harvested after intermediate-dose chemotherapy. | assess total CD34+ content. A second file using a CD34 threshold was then acquired to resolve lineage-committed subsets. The % CD34+ cells as well as cells/microliter of bone marrow was calculated using cell counts at the time of marrow harvest. Bone marrow (mean total cell dose = 3.8 x 10(5)/kg); obtained from 42 normal donors for allogeneic transplantation was first analyzed. CD34+ cells comprised a mean 1.3% of non-ficolled marrow; with 328 CD34+ cells/microliter; and mean CD34+ cells collected was 4.8 | 8979021 | [
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CD34 progenitor cell subset analyses in normal human bone marrow and marrow harvested after intermediate-dose chemotherapy. | x 10(6)/kg. While no significant differences in total cells harvested nor proportion of CD34+ cells was found; a significant decrease in CD34 cells/microliter (= 233; P = .0012) was found in cancer patients. The percentage of CD19+ and CD38+ progenitor cells was significantly increased; while CD5+ and CD71+ cells were decreased. The proportions of all other early lineage-committed CD34 subsets were not different. Measurement of lineage-committed CD34 progenitor cells is a useful technique to characterize ha | 8979021 | [
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CD34 progenitor cell subset analyses in normal human bone marrow and marrow harvested after intermediate-dose chemotherapy. | rvested marrow and PBSC; and may be applied to predict time and quality of engraftment post ablative conditioning regimens. | 8979021 | [
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Flow cytometric analysis of kappa and lambda light chain expression in evaluation of specimens for B-cell neoplasia. | Analysis of light chain expression is one of the most important determinations in flow cytometric immunophenotyping of patient specimens. Numerous technical factors; such as antibody choice and cytophilic antibody artifact; impact a laboratory's ability to perform this test. There have been conflicting reports concerning the efficacy of polyclonal versus monoclonal antibodies; as well as methods of circumventing cytophilic antibodies; indicating that a consensus has not been reached on optimal methods for l | 8979022 | [
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Flow cytometric analysis of kappa and lambda light chain expression in evaluation of specimens for B-cell neoplasia. | ight chain determination. The authors have investigated methods for light chain analysis in 104 normal donors and 366 patient specimens; comparing different anti-light chain antibodies as well as strategies for analysis of specimens with low numbers of monoclonal B cells; admixed polyclonal B cells; or cytophilic antibodies. The patient specimens were either part of the initial diagnostic evaluation of patients with suspected lymphoma; or were performed for staging or assessment of treatment of patients wit | 8979022 | [
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Flow cytometric analysis of kappa and lambda light chain expression in evaluation of specimens for B-cell neoplasia. | h known B-cell neoplasia. No monoclonality was detected in control specimens; and there was no significant difference in staining with monoclonal verses polyclonal anti-light chain antibodies. In addition; cytophilic antibody did not obscure results in normal controls. Monoclonality was detected in 106 patient specimens; with 89 showing gross involvement with a predominant monoclonal B-cell process. However; in 43% of the grossly monoclonal specimens; there was failure to detect monoclonality with at least | 8979022 | [
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Flow cytometric analysis of kappa and lambda light chain expression in evaluation of specimens for B-cell neoplasia. | one light chain antibody set; with 8% of these cases showing failure with two anti-light chain sets. This indicates the importance of antibody choice in light chain analysis. Cytophilic antibody artifact in monoclonal specimens was easily overcome by appropriate antibody combinations; obviating the need for cytophilic antibody-shedding by incubation at 37 degrees C in fetal calf serum. In 27 patient specimens with low numbers of B cells or admixed polyclonal B cells; a clonal search based on FSC and CD19 or | 8979022 | [
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Flow cytometric analysis of kappa and lambda light chain expression in evaluation of specimens for B-cell neoplasia. | CD20 expression was performed. In 17 of the 27 cases (63%); a small monoclonal population was detected among admixed polyclonal B cells. The authors conclude that multiple strategies are necessary in flow cytometric analysis for B-cell monoclonality. | 8979022 | [
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Quantification of CD1a, HLA-DR, and HLA class I expression on viable human Langerhans cells and keratinocytes. | In order to determine precisely the cellular density of surface molecules that are critical for antigen presentation in human epidermis; we utilized a quantitative immunofluorescence indirect assay and performed flow cytometric analysis of human epidermal cell (EC) suspensions. We first demonstrated that Tricolor-labeled streptavidin coupled to Cy-5 (SA-TC) was a reliable marker for non viable EC and that SA-TC+ EC accounted for the frequent nonspecific background of fluorescence due to isotype controls bin | 8979024 | [
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Quantification of CD1a, HLA-DR, and HLA class I expression on viable human Langerhans cells and keratinocytes. | ding; although Langerhans cells (LC) and Keratinocytes (Kc) express Fc receptors for IgG on their surfaces. These results indicate that quantification of cell surface antigens on human EC requires the concomitant use of a marker of viability. Multicolor flow cytometric analysis allowed us to quantify CD1 molecules and major histocompatibility complex (MHC) antigens on viable human LC and Kc. Our results demonstrated a weak expression of MHC class I molecules on viable LC (163 +/- 19 x 10(3) molecules/cell) | 8979024 | [
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Quantification of CD1a, HLA-DR, and HLA class I expression on viable human Langerhans cells and keratinocytes. | compared to viable Kc (785 +/- 110 x 10(3) molecules/cell). Mean antigen density of HLA-DR and CD1a molecules on viable LC were 579 +/- 82 x 10(3) molecules/cell and 1600 +/- 133 x 10(3) molecules/cell; respectively. Quantitative flow cytometry of viable EC may be proposed to evaluate the number of membrane antigens whose level of expression is related to cellular maturation or activation that occurs in skin diseases. | 8979024 | [
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DNA ploidy and protein content in bronchioloalveolar carcinoma multi-variable flow cytometry. | Multi-variable flow cytometry studies were done on paraffin-embedded surgical specimens from 97 patients with bronchiolalveolar carcinoma (BAC) and on 10 normal lung tissue specimens to assess the value of nuclear protein content besides DNA ploidy. Tumor tissues were stained for DNA content with propidium iodide and for protein content with fluorescein isothiocyanate. DNA ploidy measurements were successful in 87 of the 97 specimens. Twenty-four (28%) specimens were DNA diploid; and 63 (72%) were DNA nondi | 8979023 | [
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DNA ploidy and protein content in bronchioloalveolar carcinoma multi-variable flow cytometry. | ploid (DNA tetraploid or DNA aneuploid). There was no significant difference in survival between patients with DNA diploid and with DNA nondiploid tumors (P = 0.69). The protein histogram pattern was bimodal in 26/87 (30%) of patients; these had significantly shorter survival than patients with either normal or right-shift protein histogram patterns (61/87) (P = 0.0033). The nuclear protein measurement was an independent prognostic indicator when corrected for tumor grade and tumor size in a Cox model analy | 8979023 | [
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DNA ploidy and protein content in bronchioloalveolar carcinoma multi-variable flow cytometry. | sis (P = 0.04). The nuclear protein measurement correlated with nuclear size as determined by nuclear volume measurements. The combination of DNA ploidy; protein; and cell size measurements by flow cytometry provide a useful biologic basis for the variable prognosis seen with BAC tumors. | 8979023 | [
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Long lasting immunological effects of ethanol after withdrawal. | The aim of the present study was to analyze on chronic alcoholic patients the effect of ethanol (EtOH) withdrawal on the immune system through the investigation of the distribution of PB lymphoid subsets; using multiple-stainings with monoclonal antibodies and flow cytometry. For this purpose a group of 20 patients with active alcoholism without liver disease; negative for hepatitis virus; and without malnutrition was analyzed and followed for 9 months after alcohol consumption had been discontinued. Twenty | 8979026 | [
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Long lasting immunological effects of ethanol after withdrawal. | -five age- and sex-matched healthy volunteers were included in the study. The following panel of monoclonal antibodies combinations (FITC/PE/PerCP or PE-Cy5) was used: TCR alpha beta/CD3/HLA DR; CD25/CD56/CD3; TCR gamma delta/CD3/HLA DR; CD45RA/CD45R0/CD4; CD3/CD8; CD19/CD5; and CD3/CD11c. Analysis was performed on at least 1;500 events/tube at flow cytometry using the Lysys II software program. During the alcohol intake period; the most striking findings were a significant (P < 0.05) expansion of the CD8+ | 8979026 | [
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Long lasting immunological effects of ethanol after withdrawal. | T-lymphocyte subset; which coexpresses the activation associated antigens HLA DR and CD11c; as well as a significant increase in both NK-cells (CD3-/CD56+) and the T-cell subset with NK activity coexpressing CD3 and CD56 (P < 0.05 and P < 0.01; respectively). In addition; a decrease in the CD5+ B-cells (P < 0.05); associated with reduced serum gamma-globulin levels; was also observed. During alcohol withdrawal; a rapid decrease towards normal values of activated CD8+/HLA DR+ and CD11c+ T-lymphocytes was obs | 8979026 | [
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Long lasting immunological effects of ethanol after withdrawal. | erved as well as a normalization of CD19+/CD5+ B-cells and gamma-globulin serum levels; these changes might be directly related to EtOH suppression. Surprisingly; however; new immunological imbalances emerged in spite of the absence of alcohol intake. Thus; a progressive and significant expansion (P < 0.05) of CD4+ T-cells associated with an increased expression of the CD25 activation-related antigen and a preferential use of the CD45R0 isoform by CD4+ T-cells were observed. In parallel; there was an even m | 8979026 | [
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Long lasting immunological effects of ethanol after withdrawal. | ore evident increase (P < 0.01) in the number of PB NK-cells. Our results show that EtOH consumption induces changes in the immune system; its effects persisting or even becoming more evident after suppression of EtOH intake for a 9 month period. | 8979026 | [
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Quantitative determination of surface antibody binding capacities of immune subsets present in peripheral blood of healthy adult donors. | The objective of this study was to quantitate the antibody binding capacity (ABC) of CD3; CD4; CD8; CD16; and CD19 on lymphocytes and CD4 on monocytes from healthy adult donors. Peripheral blood was collected over three consecutive days and repeated in the same format two weeks later for comparison to initial measurements. Immune subsets were labeled by direct single or two-color staining in whole blood followed by lysis of erythrocytes. Fluorescence intensity measurements were made by carefully calibrating | 8979025 | [
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Quantitative determination of surface antibody binding capacities of immune subsets present in peripheral blood of healthy adult donors. | the flow cytometer and then measuring the intensity of monoclonal antibody staining on labeled cells and on Quantum Simply Cellular Microbeads. The effect of paraformaldehyde fixation on intensity measurements and coefficient of variation of thirty replicates for each phenotype were also studied. We found a small change in calculated ABC following overnight fixation with a greater change following 48 h of fixation prior to flow cytometric analysis. We found excellent precision could be achieved for measuri | 8979025 | [
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Quantitative determination of surface antibody binding capacities of immune subsets present in peripheral blood of healthy adult donors. | ng the ABC of most markers with some improvement desirable for expression of CD4 on monocytes and CD16+ lymphocytes. Between donors we found a high-low range of CD3+ = 134;349-45;905; CD4+ (lymphocytes) = 54;174-36;106; CD4+ (monocytes) = 9;246-3094; CD8+ = 268;868-190;622; CD3+CD8+ = 269;858-212;024; CD16+ = 38;307-336; and CD19+ = 25;252-11;689. For the total donor group; the observations at week 1 and week 2 were not significantly different (alpha = .05) for any of the immunophenotypes we studied. The da | 8979025 | [
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Quantitative determination of surface antibody binding capacities of immune subsets present in peripheral blood of healthy adult donors. | ta presented here continue to show that it is possible to perform quantitative intensity measurements of immune subsets when performing immunophenotyping studies. | 8979025 | [
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Flow cytometric HLA-B27 typing using CD3 gating and molecules of equivalent soluble fluorochrome (MESF) quantitation. | The determination of HLA-B27 (B27) status is important in the diagnosis of ankylosing spondylitis; Reiter's disease; and other arthropathies. Flow cytometric (FCM) typing of B27 is a relatively new method which allows for rapid turnaround time and low cost. However; different leukocyte populations may manifest significant variation in staining intensity with B27 antibodies. Therefore; gating utilizing only light scatter properties of cells may lead to high readings in some B27-negative samples. Fluorescence | 8979028 | [
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Flow cytometric HLA-B27 typing using CD3 gating and molecules of equivalent soluble fluorochrome (MESF) quantitation. | gating on CD3+ cells were postulated as a means to eliminate these anomalies. Furthermore; quantitative FCM measurements; as afforded through use of molecules of equivalent soluble fluorochrome (MESF) units; can minimize day-to-day and instrument-to-instrument variabilities in fluorescence measurements. We compared CD3 gating to light scatter gating and MESF analysis on 123 specimens in a 4-month period and found: 1) CD3 gating gave the lowest nonspecific B27 antibody binding among B27-negative subjects; 2 | 8979028 | [
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Flow cytometric HLA-B27 typing using CD3 gating and molecules of equivalent soluble fluorochrome (MESF) quantitation. | ) there was no significant difference in MESF values between CD3 gating and light scatter gating of B27-positive samples; 3) there was a wide range of B27 antibody binding fluorescence intensities among B27-positive subjects; 4) identification of patients with low B27 expression may require the use of CD3 gating in order to avoid costly confirmation testing; 5) fluorescent standard beads are stable for routine use in a clinical flow cytometry laboratory. | 8979028 | [
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Association between flow cytometric S-phase fraction and apoptotic rate in breast cancer. | Tumor heterogeneity may adversely affect the flow cytometric measurement of S-phase fraction (SPF) in breast cancer specimens; and 10-20% of breast cancer specimens are not evaluable by flow cytometry due to technical factors such as debris; high coefficients of variation; poor specimen quality; or small sample size. Therefore; we performed this study on 207 specimens of breast cancer in order to determine if the apoptotic rate (AR) could serve as a useful adjunct to flow cytometric SPF measurements in brea | 8979027 | [
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Association between flow cytometric S-phase fraction and apoptotic rate in breast cancer. | st cancers. The average AR in each specimen was determined by microscopic examination of tumor tissue that was specifically stained for apoptotic bodies by a commercially available TUNEL (Tdt-mediated dUTP digoxigenin nick end labelling) assay kit. The mean AR (4.5 +/- 3.0; n = 37) in the high SPF (> 10%) group was significantly (P < 0.01) higher than the mean AR (1.3 +/- 1.2; n = 72) in the low SPF (< 6%) group. Although the distributions of AR values in the two groups had substantial overlap; AR values gr | 8979027 | [
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Association between flow cytometric S-phase fraction and apoptotic rate in breast cancer. | eater than 5.5 per high power field (h.p.f.) were not observed in the low SPF cases but were present in 13 out of 37 cases with a high SPF. Simple linear regression analyses relating SPF to the mean AR in 57 DNA diploid cases and 41 DNA aneuploid cases yielded a minimal correlation (r2 = 0.21) between the two parameters only in the DNA aneuploid group. We conclude that an elevated AR has an association with high SPF in breast cancers; but the association is too weak to permit the general use of AR as a pred | 8979027 | [
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Association between flow cytometric S-phase fraction and apoptotic rate in breast cancer. | ictor of SPF. Our study also identified a subset of breast cancers with both a high SPF (> 10%) and a high AR (> 5.5/h.p.f.) that may warrant further investigation to determine its clinical significance. | 8979027 | [
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DNA-ploidy studies on cytological preparations from breast cancers by image analysis: comparison with Feulgen staining performed in destained Papanicolaou slides. | Image cytometric analysis of DNA ploidy status of 40 fresh primary breast carcinomas was performed in a comparative study on cytologic preparations stained directly by the Feulgen method and smears from the same tumors Feulgen-stained after Papanicolaou staining. There was a 0.82 overall concordance in diploid and aneuploid tumor classification and a good correlation (r = 0.736; P < 0.001) between the DNA indices determined by the two methods. Discordances are probably due to tumor heterogeneity. This study | 8979029 | [
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DNA-ploidy studies on cytological preparations from breast cancers by image analysis: comparison with Feulgen staining performed in destained Papanicolaou slides. | demonstrates that image cytometric DNA analysis on previously routine-stained cytologic preparations is feasible and reliable for pretreatment DNA analysis and suggests potential applications in retrospective studies with cytologic material from archives. | 8979029 | [
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Measurement of T-cell CD69 expression: a rapid and efficient means to assess mitogen- or antigen-induced proliferative capacity in normals. | We have analyzed the expression of the activation antigen CD69 on normal human T cells by flow cytometry following stimulation with mitogens and recall antigen. These data were compared to parallel studies assessing the proliferative response using the 3H-thymidine (3H-TdR) incorporation assay. Three different mitogens (PHA; ConA; and CD2/CD2R) induced maximal expression of CD69 at 24 h; which remained stable throughout the 72 h culture period. The mitogen-stimulated cells initiated DNA synthesis as determi | 8979031 | [
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Measurement of T-cell CD69 expression: a rapid and efficient means to assess mitogen- or antigen-induced proliferative capacity in normals. | ned by the 3H-TdR assay (72 h) while nonstimulated cells failed to upregulate CD69 or incorporate 3H-TdR. We next compared T cell CD69 expression (n = 12) following stimulation with either CD2/CD2R (5 micrograms/ml) or the recall antigen; tetanus toxoid (1:1500). Cell proliferation was determined by the 3H-TdR assay at 72 h (CD2/CD2R) or 120 h (tetanus toxoid). Evaluation of CD69 expression at 6 h predicted CD2/CD2R but not tetanus responder status as defined by 3H-TdR incorporation. However; when 4 known t | 8979031 | [
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Measurement of T-cell CD69 expression: a rapid and efficient means to assess mitogen- or antigen-induced proliferative capacity in normals. | etanus responders (3H-TdR) were evaluated over time; it was found that at 48 h the fluorescence intensity (of CD69) on tetanus-stimulated CD3+ cells increased markedly compared with nonstimulated cells (range of increase 43-850%). One individual whose cells failed to respond to tetanus toxoid (3H-TdR and CD69) did respond normally to CD2/CD2R. These observations suggest that flow cytometric evaluation of T cell CD69 expression following mitogen (6 h) or antigen (48 h) stimulation may provide an accurate scr | 8979031 | [
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Measurement of T-cell CD69 expression: a rapid and efficient means to assess mitogen- or antigen-induced proliferative capacity in normals. | een of T-cell responsiveness in normals. | 8979031 | [
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Automated assessment of numerical chromosomal aberrations in paraffin embedded prostate tumor cells stained by in situ hybridization. | We investigated the feasibility of automated counting of in situ hybridization signals (ISH) in interphase cells isolated from paraffin embedded prostate tissue. In total; 34 specimens from 7 patients with prostate cancer were stained with probes specific for the centromeric regions of chromosomes Y; 1; 7; 8; 10; and 15; using an immunoperoxidase based technique suitable for bright-field microscopy. Enumeration of the number of ISH spots of 500 nuclei per specimen was performed (1) using an automatic system | 8979030 | [
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Automated assessment of numerical chromosomal aberrations in paraffin embedded prostate tumor cells stained by in situ hybridization. | developed without any human intervention and (2) using the same system; but including verification of the counts based on visual inspection of the stored images. As reference from each specimen; 200 cell nuclei were evaluated manually; using conventional microscopy. A typical analysis procedure (including user verification) took 35 min. The difference (root mean error) between the automated counting and the counting after visual interaction was relatively small (15%). The percentage of cells with incorrect | 8979030 | [
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Automated assessment of numerical chromosomal aberrations in paraffin embedded prostate tumor cells stained by in situ hybridization. | counts by automated analysis was 20.2%; a number that could easily be improved by user interaction. Detection of cells with aneusomy proved to be more sensitive compared to the routine manual counting; in cases where aberrant frequencies were low. Automated counting of samples with low frequencies (< 10%) resulted in a higher frequency of aberrant cells in 9 of 11 cases; probably due to the fact that an unbiased cell selection is guaranteed. Automated assessment of ISH signals is considered useful for the | 8979030 | [
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Automated assessment of numerical chromosomal aberrations in paraffin embedded prostate tumor cells stained by in situ hybridization. | evaluation of chromosomal aberrations in prostate tumor cells; provided that the counts are visually confirmed. | 8979030 | [
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Enhanced flow cytometric method for counting very low numbers of white cells in platelet products. | The increased demand for leukocyte-reduced platelet products has prompted actions both by blood suppliers and device manufacturers to develop effective quality assurance methods and improved devices for leukocyte-reduced platelet preparation. White cell (WBC) counting methods capable of counting WBCs substantially below 1;000 WBC/mL enhance these activities. The purpose of this study was to enhance and validate a method capable of counting 5-10 WBC/mL in platelets.We added marker chicken red blood cells (cR | 8979032 | [
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Enhanced flow cytometric method for counting very low numbers of white cells in platelet products. | BC) at a known concentration to an aliquot of platelet concentrate containing propidium iodide stain solution (PI). A modified BD FACScan was used to analyze this mixture. The ratio of cRBC and WBC events observed was used with the cRBC concentration to calculate the WBC concentration. Validation was performed by analyzing standard curves prepared with leukocyte-depleted platelet diluent. Methods of preparing leukocyte-reduced platelets by apheresis and filtration were evaluated using this method.The method | 8979032 | [
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Enhanced flow cytometric method for counting very low numbers of white cells in platelet products. | was linear from 5;000 WBC/mL to 10 WBC/mL (r2 = 0.983). Residual WBCs in the platelet diluent hampered validation below 10 WBC/mL. Repeatability (CV) was 44% at 8 WBC/mL and 6.5% at 1;000 WBC/mL. The FACScan required frequent mixing of the sample and flushing with cleaning agents during acquisition.This method has good linearity and reproducibility from 10-5;000 WBC/mL for platelets. It is suited for research and development work; and may be a useful adjunct method for quality assurance in the blood center | 8979032 | [
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Enhanced flow cytometric method for counting very low numbers of white cells in platelet products. | . | 8979032 | [
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Preliminary observations on the development of auditory sensitivity in infants with Down syndrome. | Hearing loss commonly is associated with Down syndrome; but little is known about the development of auditory sensitivity in individuals with Down syndrome. This study had two objectives: 1) to determine whether an observer-based procedure can be used to assess the behavioral sensitivity of infants with Down syndrome; and 2) to provide preliminary information about the early course of hearing development among infants with Down syndrome.Behavioral measures of sensitivity were made in 16 infants with Down sy | 8979034 | [
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Preliminary observations on the development of auditory sensitivity in infants with Down syndrome. | ndrome using an observer-based procedure. Ten of the infants were followed longitudinally between 2 and 12 mo of age. All infants passed a screening auditory brain stem response at 20 dB nHL during the study and completed screening tympanometry at each test session. The infants detected a 4000 Hz tone at levels ranging from 25 to 50 dB SPL; psychometric functions and thresholds for the tone were obtained.Infants with Down syndrome completed 80% of the test sessions they began; a rate similar to that seen in | 8979034 | [
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Preliminary observations on the development of auditory sensitivity in infants with Down syndrome. | normally developing infants in the same type of study. Performance improved with increasing stimulus level; as one would expect; and thresholds were obtained from 15 of 16 infants for at least one age. The performance of infants with Down syndrome generally improved with age. The sensitivity of 2- to 3-mo-olds was poorer than that of older infants; but little if any improvement in sensitivity occurred between 4 and 12 mo. This pattern is similar to that seen in normally developing infants. Thresholds of in | 8979034 | [
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Preliminary observations on the development of auditory sensitivity in infants with Down syndrome. | fants with Down syndrome were 10 to 25 dB higher than those reported for normally developing infants. The psychometric functions of infants with Down syndrome were shallower than the psychometric functions of normally developing infants; and the slope of the psychometric functions did not change with age. Infants with Down syndrome achieved only 75 to 80% correct at any of the levels tested; 5 to 10% poorer than reported for other infants. These characteristics of the psychometric functions of the infants w | 8979034 | [
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Preliminary observations on the development of auditory sensitivity in infants with Down syndrome. | ith Down syndrome suggest that they are inattentive during testing more often than are normally developing infants.An observer-based procedure can be used to obtain reasonable thresholds from infants with Down syndrome who are as young as 2 mo of age. Both sensory and nonsensory factors could contribute to the threshold elevation seen in infants with Down syndrome. At least on preliminary examination; the course of auditory sensitivity development of infants with Down syndrome is qualitatively similar to th | 8979034 | [
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Apoptosis of cells in aged samples as detected by the ProCOUNT reagent. | An unusual population of high side scatter; low nucleic acid dye binding; dim CD45 cells was found in aged blood samples stained with the ProCOUNT reagent. Cell surface staining showed that these cells have the surface phenotype of neutrophils. However; they have decreased expression of several surface antigens; bind annexin V; and stain more dimly than normal neutrophils with LDS-751. These characteristics indicate that the cells have become apoptotic. The decreased expression of the CD45 antigen on apopto | 8979033 | [
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Apoptosis of cells in aged samples as detected by the ProCOUNT reagent. | tic neutrophils could have an impact on some methods for enumerating CD34-positive progenitor cells. Absolute CD34-positive cell counts are frequently obtained by multiplying CD34-positive cells as a percentage of the total cells by the white blood cell count from a hematologyanalyzer. Cells staining dimly with CD45 may not pass a flow cytometer threshold set on this parameter but appear to be detected by a hematology instrument thresholding on cell size. Thus the white blood cell counts from the two source | 8979033 | [
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Apoptosis of cells in aged samples as detected by the ProCOUNT reagent. | s may not be identical; introducing error into the calculated absolute CD34 count. Absolute counts of CD34 cells based on simultaneous acquisition of a counting bead are not affected by the presence of this population; but purity estimates can be affected if the possible presence of these cells is not considered in aged samples. | 8979033 | [
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