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--- |
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size_categories: |
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- 1M<n<10M |
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pretty_name: CF/MS Homo sapiens Elution Profile PPI Dataset |
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tags: |
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- biology |
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- chemistry |
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dataset_summary: >- |
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Processed data from several *Homo sapiens* protein co-fractionation mass |
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spectrometry (CF/MS) experiments, as well as positive/negative protein-protein |
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interaction (PPI) labels for each pair. Collated, maintained by the Drew Lab at |
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University of Illinois at Chicago. |
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citation_bibtex: |
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- >- |
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@article{Connelly2018, title = {Analysis of Human Nuclear Protein Complexes by |
|
Quantitative Mass Spectrometry Profiling}, volume = {18}, ISSN = {1615-9861}, |
|
url = {http://dx.doi.org/10.1002/pmic.201700427}, DOI = |
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{10.1002/pmic.201700427}, number = {11}, journal = {PROTEOMICS}, publisher = |
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{Wiley}, author = {Connelly, Katelyn E. and Hedrick, Victoria and Paschoal |
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Sobreira, Tiago Jose and Dykhuizen, Emily C. and Aryal, Uma K.}, year = |
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{2018}, month = may} |
|
- >- |
|
@article{Kirkwood2013, title = {Characterization of Native Protein Complexes |
|
and Protein Isoform Variation Using Size-fractionation-based Quantitative |
|
Proteomics}, volume = {12}, ISSN = {1535-9476}, url = |
|
{http://dx.doi.org/10.1074/mcp.M113.032367}, DOI = {10.1074/mcp.m113.032367}, |
|
number = {12}, journal = {Molecular & Cellular Proteomics}, publisher = |
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{Elsevier BV}, author = {Kirkwood, Kathryn J. and Ahmad, Yasmeen and |
|
Larance, Mark and Lamond, Angus I.}, year = {2013}, month = dec, pages = |
|
{3851–3873}} |
|
- >- |
|
@article{Larance2016, title = {Global Membrane Protein Interactome Analysis |
|
using In vivo Crosslinking and Mass Spectrometry-based Protein Correlation |
|
Profiling}, volume = {15}, ISSN = {1535-9476}, url = |
|
{http://dx.doi.org/10.1074/mcp.O115.055467}, DOI = {10.1074/mcp.o115.055467}, |
|
number = {7}, journal = {Molecular & Cellular Proteomics}, publisher = |
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{Elsevier BV}, author = {Larance, Mark and Kirkwood, Kathryn J. and Tinti, |
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Michele and Brenes Murillo, Alejandro and Ferguson, Michael A.J. and |
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Lamond, Angus I.}, year = {2016}, month = jul, pages = {2476–2490}} |
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- >- |
|
@article{Mallam2019, title = {Systematic Discovery of Endogenous Human |
|
Ribonucleoprotein Complexes}, volume = {29}, ISSN = {2211-1247}, url = |
|
{http://dx.doi.org/10.1016/j.celrep.2019.09.060}, DOI = |
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{10.1016/j.celrep.2019.09.060}, number = {5}, journal = {Cell Reports}, |
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publisher = {Elsevier BV}, author = {Mallam, Anna L. and Sae-Lee, Wisath and |
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Schaub, Jeffrey M. and Tu, Fan and Battenhouse, Anna and Jang, Yu Jin and |
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Kim, Jonghwan and Wallingford, John B. and Finkelstein, Ilya J. and |
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Marcotte, Edward M. and Drew, Kevin}, year = {2019}, month = oct, pages = |
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{1351--1368.e5}} |
|
- >- |
|
@article{Moutaoufik2019, title = {Rewiring of the Human Mitochondrial |
|
Interactome during Neuronal Reprogramming Reveals Regulators of the |
|
Respirasome and Neurogenesis}, volume = {19}, ISSN = {2589-0042}, url = |
|
{http://dx.doi.org/10.1016/j.isci.2019.08.057}, DOI = |
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{10.1016/j.isci.2019.08.057}, journal = {iScience}, publisher = {Elsevier BV}, |
|
author = {Moutaoufik, Mohamed Taha and Malty, Ramy and Amin, Shahreen and |
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Zhang, Qingzhou and Phanse, Sadhna and Gagarinova, Alla and Zilocchi, Mara |
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and Hoell, Larissa and Minic, Zoran and Gagarinova, Maria and Aoki, |
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Hiroyuki and Stockwell, Jocelyn and Jessulat, Matthew and Goebels, Florian |
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and Broderick, Kirsten and Scott, Nichollas E. and Vlasblom, James and |
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Musso, Gabriel and Prasad, Bhanu and Lamantea, Eleonora and Garavaglia, |
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Barbara and Rajput, Alex and Murayama, Kei and Okazaki, Yasushi and |
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Foster, Leonard J. and Bader, Gary D. and Cayabyab, Francisco S. and Babu, |
|
Mohan}, year = {2019}, month = sep, pages = {1114–1132}} |
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- >- |
|
@article{Wan2015, title = {Panorama of ancient metazoan macromolecular |
|
complexes}, volume = {525}, ISSN = {1476-4687}, url = |
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{http://dx.doi.org/10.1038/nature14877}, DOI = {10.1038/nature14877}, number = |
|
{7569}, journal = {Nature}, publisher = {Springer Science and Business Media |
|
LLC}, author = {Wan, Cuihong and Borgeson, Blake and Phanse, Sadhna and |
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Tu, Fan and Drew, Kevin and Clark, Greg and Xiong, Xuejian and Kagan, |
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Olga and Kwan, Julian and Bezginov, Alexandr and Chessman, Kyle and Pal, |
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Swati and Cromar, Graham and Papoulas, Ophelia and Ni, Zuyao and Boutz, |
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Daniel R. and Stoilova, Snejana and Havugimana, Pierre C. and Guo, Xinghua |
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and Malty, Ramy H. and Sarov, Mihail and Greenblatt, Jack and Babu, Mohan |
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and Derry, W. Brent and R. Tillier, Elisabeth and Wallingford, John B. and |
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Parkinson, John and Marcotte, Edward M. and Emili, Andrew}, year = {2015}, |
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month = sep, pages = {339–344}} |
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citation_apa: |
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- >- |
|
Connelly, K. E., Hedrick, V., Paschoal Sobreira, T. J., Dykhuizen, E. C., & |
|
Aryal, U. K. (2018). Analysis of Human Nuclear Protein Complexes by |
|
Quantitative Mass Spectrometry Profiling. Proteomics, 18(11), e1700427. |
|
https://doi.org/10.1002/pmic.201700427 |
|
- Kirkwood, K. J., Ahmad, Y., Larance, M., & Lamond, A. I. (2013). Characterization of native protein complexes and protein isoform variation using size-fractionation-based quantitative proteomics. Molecular & cellular proteomics: MCP, 12(12), 3851–3873. https://doi.org/10.1074/mcp.M113.032367 |
|
- Larance, M., Kirkwood, K. J., Tinti, M., Brenes Murillo, A., Ferguson, M. A., & Lamond, A. I. (2016). Global Membrane Protein Interactome Analysis using In vivo Crosslinking and Mass Spectrometry-based Protein Correlation Profiling. Molecular & cellular proteomics: MCP, 15(7), 2476–2490. https://doi.org/10.1074/mcp.O115.055467 |
|
- >- |
|
Mallam, A. L., Sae-Lee, W., Schaub, J. M., Tu, F., Battenhouse, A., Jang, Y. |
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J., Kim, J., Wallingford, J. B., Finkelstein, I. J., Marcotte, E. M., & Drew, |
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K. (2019). Systematic Discovery of Endogenous Human Ribonucleoprotein |
|
Complexes. Cell reports, 29(5), 1351–1368.e5. |
|
https://doi.org/10.1016/j.celrep.2019.09.060 |
|
- >- |
|
Moutaoufik, M. T., Malty, R., Amin, S., Zhang, Q., Phanse, S., Gagarinova, A., |
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Zilocchi, M., Hoell, L., Minic, Z., Gagarinova, M., Aoki, H., Stockwell, J., |
|
Jessulat, M., Goebels, F., Broderick, K., Scott, N. E., Vlasblom, J., Musso, |
|
G., Prasad, B., Lamantea, E., … Babu, M. (2019). Rewiring of the Human |
|
Mitochondrial Interactome during Neuronal Reprogramming Reveals Regulators of |
|
the Respirasome and Neurogenesis. iScience, 19, 1114–1132. |
|
https://doi.org/10.1016/j.isci.2019.08.057 |
|
- >- |
|
Wan, C., Borgeson, B., Phanse, S., Tu, F., Drew, K., Clark, G., Xiong, X., |
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Kagan, O., Kwan, J., Bezginov, A., Chessman, K., Pal, S., Cromar, G., |
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Papoulas, O., Ni, Z., Boutz, D. R., Stoilova, S., Havugimana, P. C., Guo, X., |
|
Malty, R. H., … Emili, A. (2015). Panorama of ancient metazoan macromolecular |
|
complexes. Nature, 525(7569), 339–344. https://doi.org/10.1038/nature14877 |
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--- |
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# CF/MS Elution Profile PPI Dataset |
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Proteins are the functional basis of life, but it is often their interactions with other proteins which gives rise to said functions. Therefore, we are often interested in whether two proteins participate in the same *protein complex*, or if they **'co-complex'**. Co-fractionation mass spectrometry (CF/MS) is a high-throughput method for determining whether proteins form complexes. If they do, both proteins will typically separate out into the same fractions, or **'co-elute'**, during column chromatography experiments. As a result, their abundances will be *highly correlated* across all the fractions measured. CF/MS leverages this fact to identify new protein complexes by attempting to statistically correlate the elution profiles of groups of proteins. Typically, we use Pearson correlation coefficient to determine correlation between protein pairs. While this often works quite well, Pearson is a linear function. Current research is exploring whether there are non-linear, higher-order signals between these elution profiles that might have better predictive power than Pearson. As deep learning models excel at estimating non-linear relationships in data, the goal of this dataset is to act as training data for such models, especially **Siamese networks**. |
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|
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Includes processed data from several *Homo sapiens* protein **co-fractionation mass spectrometry (CF/MS)** experiments, as well as positive/negative protein-protein interaction (PPI) labels for each pair. |
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|
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Collated, maintained by **Drew Lab at University of Illinois at Chicago** |
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|
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[Drew Lab webpage](https://ksdrew.github.io/) |
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|
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## File formats |
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- **The .elut file**: A **.elut** file is a TSV-like format containing raw count data from a chromatographic fractionation experiment. Each row in a **.elut** file shows the abundance of a single protein across the collected fractions (columns). Generally speaking, these fractions are collected over time. However, different chromatographic columns can separate proteins along different axes. For example, *Size-eclusion chromatography (SEC)* will mostly separate proteins into fractions according to their *size*; *Ion-exchange chromatography (IEX)* will separate them into fractions according to their *charge*. Each file in this dataset comes from one of these two column separation methods and is named accordingly ('...xx_SEC_xx...' / '...xx_IEX_xx...'. We refer to a given protein's (row's) count data across all fractions (columns) as that protein's **elution trace**. To summarize: |
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- A given row contains count data for a specific protein |
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- A row's *first column* contains its associated **protein ID** |
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- A row's *subsequent columns* contain that protein's count data from the fractionation experiment |
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- **Note: The user may notice that the first row in a .elut file is one column longer than subsequent rows. This is because the first row contains row names (protein IDs), and the first column contains column names (fraction IDs). Therefore, cell 'A0' is empty.** |
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## File structure |
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- The **.elut** files each contain a collection elution traces for proteins from a given CF/MS experiment. These can be paired to make sample data. A complete list of data sources can be found at the bottom of this README |
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- The **.txt** files contain line-wise specification of protein complexes used to generate positive/negative labels. These can be used to direct the pairing of elution traces into data points. |
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- **intact_complex_merge_20230309.train_ppis.txt**: List of positive PPIs for training data |
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- **intact_complex_merge_20230309.test_ppis.txt**: List of positive PPIs for testing data |
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- **intact_complex_merge_20230309.neg_train_ppis.txt**: List of negative PPIs for training data |
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- **intact_complex_merge_20230309.neg_test_ppis.txt**: List of negative PPIs for testing data |
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- **intact_complex_merge_20230309.train.txt** Line-wise list of protein complexes |
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### List of publications/experiments from which this dataset was assembled: |
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Connelly, K. E., Hedrick, V., Paschoal Sobreira, T. J., Dykhuizen, E. C., & Aryal, U. K. (2018). Analysis of Human Nuclear Protein Complexes by Quantitative Mass Spectrometry Profiling. Proteomics, 18(11), e1700427. https://doi.org/10.1002/pmic.201700427 |
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- T98G_glioblastoma_multiforme_cells_SEC_Conelly_2018_Bio1.elut |
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- T98G_glioblastoma_multiforme_cells_SEC_Conelly_2018_Bio2.elut |
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Kirkwood, K. J., Ahmad, Y., Larance, M., & Lamond, A. I. (2013). Characterization of native protein complexes and protein isoform variation using size-fractionation-based quantitative proteomics. Molecular & cellular proteomics : MCP, 12(12), 3851–3873. https://doi.org/10.1074/mcp.M113.032367 |
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- U2OS_cells_SEC_Kirkwood_2013_rep1.elut |
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- U2OS_cells_SEC_Kirkwood_2013_rep2.elut |
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- U2OS_cells_SEC_Kirkwood_2013_rep3.elut |
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Larance, M., Kirkwood, K. J., Tinti, M., Brenes Murillo, A., Ferguson, M. A., & Lamond, A. I. (2016). Global Membrane Protein Interactome Analysis using In vivo Crosslinking and Mass Spectrometry-based Protein Correlation Profiling. Molecular & cellular proteomics : MCP, 15(7), 2476–2490. https://doi.org/10.1074/mcp.O115.055467 |
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- U2OS_cells_SEC_Larance_2016_PT3281S1.elut |
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- U2OS_cells_SEC_Larance_2016_PT3441S1.elut |
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- U2OS_cells_SEC_Larance_2016_PT3442S1.elut |
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- U2OS_cells_SEC_Larance_2016_PT3701S1.elut |
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- U2OS_cells_SEC_Larance_2016_PTSS3801.elut |
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- U2OS_cells_SEC_Larance_2016_PTSS3802.elut |
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Mallam, A. L., Sae-Lee, W., Schaub, J. M., Tu, F., Battenhouse, A., Jang, Y. J., Kim, J., Wallingford, J. B., Finkelstein, I. J., Marcotte, E. M., & Drew, K. (2019). Systematic Discovery of Endogenous Human Ribonucleoprotein Complexes. Cell reports, 29(5), 1351–1368.e5. https://doi.org/10.1016/j.celrep.2019.09.060 |
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- HEK_293_T_cells_SEC_Mallam_2019_C1.elut |
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- HEK_293_T_cells_SEC_Mallam_2019_C2.elut |
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Moutaoufik, M. T., Malty, R., Amin, S., Zhang, Q., Phanse, S., Gagarinova, A., Zilocchi, M., Hoell, L., Minic, Z., Gagarinova, M., Aoki, H., Stockwell, J., Jessulat, M., Goebels, F., Broderick, K., Scott, N. E., Vlasblom, J., Musso, G., Prasad, B., Lamantea, E., … Babu, M. (2019). Rewiring of the Human Mitochondrial Interactome during Neuronal Reprogramming Reveals Regulators of the Respirasome and Neurogenesis. iScience, 19, 1114–1132. https://doi.org/10.1016/j.isci.2019.08.057 |
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- NTera2_embryonal_carcinoma_stem_cells_IEX_Moutaoufik_2019_2_R1.elut |
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- NTera2_embryonal_carcinoma_stem_cells_IEX_Moutaoufik_2019_2_R2.elut |
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- NTera2_embryonal_carcinoma_stem_cells_IEX_Moutaoufik_2019_R1.elut |
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- NTera2_embryonal_carcinoma_stem_cells_IEX_Moutaoufik_2019_R2.elut |
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- NTera2_embryonal_carcinoma_stem_cells_SEC_Moutaoufik_2019_2_R1.elut |
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- NTera2_embryonal_carcinoma_stem_cells_SEC_Moutaoufik_2019_2_R2.elut |
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- NTera2_embryonal_carcinoma_stem_cells_SEC_Moutaoufik_2019_R1.elut |
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- NTera2_embryonal_carcinoma_stem_cells_SEC_Moutaoufik_2019_R2.elut |
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Wan, C., Borgeson, B., Phanse, S., Tu, F., Drew, K., Clark, G., Xiong, X., Kagan, O., Kwan, J., Bezginov, A., Chessman, K., Pal, S., Cromar, G., Papoulas, O., Ni, Z., Boutz, D. R., Stoilova, S., Havugimana, P. C., Guo, X., Malty, R. H., … Emili, A. (2015). Panorama of ancient metazoan macromolecular complexes. Nature, 525(7569), 339–344. https://doi.org/10.1038/nature14877 |
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- CB660_neural_stem_cell_IEX_Wan_2015.elut |
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- G166_glioma_stem_cell_IEX_Wan_2015_Hs_HCW_2.elut |
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- G166_glioma_stem_cell_IEX_Wan_2015_Hs_HCW_3.elut |
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- IEX_Wan_2015_Hs_HCW_4.elut |
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- IEX_Wan_2015_Hs_HCW_5.elut |
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- IEX_Wan_2015_Hs_HCW_6.elut |
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- IEX_Wan_2015_Hs_HCW_7.elut |
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- IEX_Wan_2015_Hs_HCW_8.elut |
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- IEX_Wan_2015_Hs_HCW_9.elut |
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- IEX_Wan_2015_Hs_IEX_1.elut |
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- IEX_Wan_2015_Hs_IEX_2.elut |
